473 results about "Trimer" patented technology

The invention discloses an antistatic polyester fiber and a preparation method thereof, and relates to the field of fibers. When the antistatic polyester fiber is prepared, N, N-dimethylethanolamine reacts with allyl chloride, and then dichloro phthalocyanine silicon is grafted to obtain modified silicon phthalocyanine; polymerizing terephthalic acid, itaconic acid and ethylene glycol, and reacting with fluorescein isothiocyanate and modified silicon phthalocyanine in sequence to obtain modified polyester chips; the preparation method comprises the following steps: reacting phosphonitrilic chloride trimer with 3-hydroxy-5-nitropyridazine, and reducing to obtain a flame retardant; and carrying out melt spinning on the modified polyester chips and a flame retardant, and reacting to obtain the antistatic polyester fiber. The antistatic polyester fiber prepared by the invention has excellent antistatic property, antibacterial property, flame retardance and mechanical property.

The invention discloses a CD40L-IL-2 fusion protein, preparation thereof and application of the CD40L-IL-2 fusion protein in preparation of drugs for treating tumors. The fusion protein comprises a CD40L trimer or a variant thereof, and a cytokine IL-2 or a variant thereof; the CD40L trimer comprises three CD40L monomers which are connected with one another. The fusion protein provided by the invention can synergistically activate APC-dependent antigen presentation and T cell-mediated immune killing to form closed-loop anti-tumor immune response of antigen presentation-immune initiation-effect amplification, and an effective strategy is provided for tumor treatment.

The invention discloses a novel coronavirus neutralizing antibody, a detection kit and application of the novel coronavirus neutralizing antibody. The amino acid sequence of a heavy chain variable region of the neutralizing antibody is shown as SEQ ID No.1, and the amino acid sequence of a light chain variable region of the neutralizing antibody is shown as SEQ ID No.2. The antibody with mature affinity is screened through bioinformatics analysis of a single B cell, the antibody screening process is optimized by combining single cell RNA sequencing, VDJ rearrangement analysis and somatic cell hypermutation research, blindness of a traditional method is avoided, and the accuracy and effectiveness of antibody screening are improved. According to the neutralizing antibody GR75 provided by the invention, a heavy chain variable region and a light chain variable region of the neutralizing antibody GR75 can be specifically combined with an RBD structural domain of the SARS-CoV-2 and an S-Trimer structural domain of an Omicro variant, so that a broad-spectrum neutralizing effect on the SARS-CoV-2 virus and the variant thereof is realized. The binding activity of the antibody GR75 to S-Trimer and RBD under 2-fold and 300-fold dilution conditions is obviously superior to that of other antibodies, which indicates that the antibody GR75 has high affinity and dilution stability and is suitable for clinical large-dose administration.

The invention discloses a novel coronavirus neutralizing antibody GR46, a detection kit and application of the novel coronavirus neutralizing antibody GR46. The amino acid sequence of a heavy chain variable region of the neutralizing antibody is shown as SEQ ID No.1, and the amino acid sequence of a light chain variable region of the neutralizing antibody is shown as SEQ ID No.2. The antibody with mature affinity is screened through bioinformatics analysis of a single B cell, the antibody screening process is optimized by combining single cell RNA sequencing, VDJ rearrangement analysis and somatic cell hypermutation research, blindness of a traditional method is avoided, and the accuracy and effectiveness of antibody screening are improved. According to the neutralizing antibody GR46 provided by the invention, a heavy chain variable region and a light chain variable region of the neutralizing antibody GR46 can be specifically combined with an RBD structural domain of the SARS-CoV-2 and an S-Trimer structural domain of an Omicro variant, so that a broad-spectrum neutralizing effect on the SARS-CoV-2 virus and the variant thereof is realized. The binding activity of the antibody GR46 to S-Trimer and RBD under 2-time and 300-time dilution conditions is obviously superior to that of other antibodies, which indicates that the antibody GR46 has high affinity and dilution stability and is suitable for clinical large-dose administration.

The invention belongs to the field of veterinary drugs, and relates to an avian infectious bronchitis virus antigen recombinant protein S-Trimer and a subunit vaccine thereof. The invention provides an avian infectious bronchitis virus antigen recombinant protein S-Trimer. The amino acid sequence of the antigen recombinant protein S-Trimer is as shown in SEQ ID No. 4. The invention provides an infectious bronchitis virus antigen. The infectious bronchitis virus antigen is of a trimer structure of the recombinant protein S-Trimer. The invention provides a chicken infectious bronchitis virus subunit vaccine. The subunit vaccine comprises a pharmaceutically acceptable carrier and an immune dose of the chicken infectious bronchitis virus antigen. The vaccine is high in safety, good in immunogenicity and stable in batches, and can provide complete protection for attacking the infectious bronchitis virus.

The invention discloses an antigen fusion protein and application thereof in preparation of a live vaccine for preventing infectious bursal disease. Specifically disclosed are antigen fusion proteins comprising a VP2 protein, a trimer tag, and a C3d protein. The invention also discloses recombinant Eimeria heap constructed by using the antigen fusion protein and application of the recombinant Eimeria heap in preparation of IBDV live vaccines. The oocyst of the recombinant Eimeria heap can effectively induce humoral immune response aiming at IBDV as a vaccine active ingredient, the level of an induced antibody is obviously higher than that of an insect strain expressed by a single VP2 antigen, viruses can be more effectively neutralized, the immune protection efficacy is improved, the safety is high, and the oocyst has the potential of serving as a live vector genetic engineering vaccine. The IBDV live vaccine can be orally taken through drinking water or feed, vaccination is simple, large-scale immunization of chicken flocks can be achieved, and the IBDV live vaccine is economical and efficient and has a wide prospect of being applied to prevention and control of the IBDV of the chicken flocks.

The invention discloses an indoor flame-retardant insulated flexible wire and a preparation method thereof, belongs to the technical field of flexible wires, and is used for solving the technical problem that the insulating property and flame-retardant property of an indoor flexible wire in the prior art need to be further improved. The cable comprises a wire layer formed by twisting a plurality of copper wires, an insulating layer and an outer sheath layer, a modified aminating agent taking a siloxane chain segment as a main body is prepared, polyvinyl chloride is aminated and hybridized with a space chain segment of the polyvinyl chloride at the same time, after phosphorus-hydrogen addition and surface modification, the hybridized polyvinyl chloride is obtained, and through sodium hypophosphite and 1, 2, 4-trimethyl-1, 3-pentanediol, the flame retardant is added to the polyvinyl chloride, so that the cable is formed. The preparation method comprises the following steps: carrying out phosphorus-hydrogen addition on 2, 3-dihydroxystyrene to obtain a modified cross-linking agent, forming a spherical structure by the modified cross-linking agent and phosphonitrilic chloride trimer under triethylamine and ultrasonic conditions, carrying out surface modification to obtain flame-retardant microspheres, and carrying out melt extrusion on the modified cross-linking agent and phosphonitrilic chloride trimer as main materials and auxiliary materials of an outer sheath layer to finally prepare the flame-retardant insulated flexible wire.

The invention discloses a novel plasmid vector capable of increasing CHO foreign protein expression quantity and construction and application thereof, and belongs to the technical field of gene engineering. The plasmid vector pCHO6GS belongs to a brand-new plasmid system, and the expression quantity of a common monoclonal antibody and recombinant protein (such as H5HA trimer) difficult to express in CHO can be increased by at least more than one time, so that the expression quantity of foreign protein is increased beyond expectation through the novel plasmid vector; and the method also plays an extremely important role in reducing cost and improving efficiency of various protein products taking CHO as a cell factory.

The present invention relates to the technical field of biomedicine. Specifically provided are a respiratory syncytial virus (RSV) Pre-F recombinant protein nanoparticle vaccine and a preparation method therefor. By performing amino acid mutation on an RSV Pre-F protein, and performing fusion expression on the mutated Pre-F protein and ferritin particles in eukaryotic cells, PreF-Ferritin fusion protein nanoparticles, in which eight Pre-F protein trimers are densely displayed on the surface of each ferritin particle, are obtained. By stabilizing and exposing antigen epitopes required to be displayed and disrupting or masking unrequired antigen epitopes, the PreF-Ferritin fusion protein nanoparticles effectively improve the immunogenicity and the production stability of the antigen. Experiments show that: when the PreF-Ferritin fusion protein is injected into mice, high-titer protective sera can be acquired, and the mice sera can yield relatively high neutralizing titers against RSV.

The present invention relates to combination therapies employing tumor targeted anti-CEA / CD3 bispecific antibodies and / or agents blocking PD-L1 / PD-1 interaction in combination with 4-1BB (CD137) agonists, in particular 4-1BBL trimer containing antigen binding molecules that also target FAP, the use of these combination therapies for the treatment of cancer and methods of using the combination therapies.

The invention relates to the technical field of adhesives, and provides a polyurethane adhesive and a preparation method and a use method thereof.The polyurethane adhesive comprises a first component and a second component, the first component is prepared from a polyurethane prepolymer, isocyanate, pigment filler and a thixotropic agent; the second component is prepared from the following raw materials: polyol, a water removal agent, a pigment filler, a first catalyst and a second catalyst; the polyurethane prepolymer is obtained through reaction of a low-activity isocyanate monomer and a chain extender, the low-activity isocyanate monomer is selected from at least one of isophorone diisocyanate, hexamethylene diisocyanate, dicyclohexylmethane diisocyanate and a hexamethylene diisocyanate tripolymer, and the chain extender is selected from at least one of isophorone diisocyanate, hexamethylene diisocyanate, dicyclohexylmethane diisocyanate and hexamethylene diisocyanate tripolymer. The first catalyst is selected from at least one of an organic tin catalyst and an organic bismuth catalyst, and the second catalyst is selected from at least one of amine catalysts.

The invention discloses a polyurethane repairing material for underground engineering concrete, and relates to the technical field of engineering leakage treatment. A component A is a polyether polyol system containing a silane coupling agent and active inorganic powder, a component B is an emulsion of a modified MDI prepolymer-aliphatic isocyanurate tripolymer and an alkaline activator, the two components are instantaneously converged in a three-cavity grouting gun and a constant-temperature air three-runner according to the mass ratio of 0.8-1.2: 1, gel is formed in the environment of-10 DEG C to 50 DEG C for 90 s, and water is stopped in 10 min. The micro-foaming expansion rate is 2-4 times, geopolymers are synchronously generated, organic-inorganic dual-curing interlocking is achieved, the closed porosity is larger than or equal to 95%, and the compression strength is gt; the permeability coefficient is less than or equal to 10-m / s. Silane chemical bridging enables the bonding strength to be higher than the splitting strength of the base material.

The invention discloses an RSV pre-F tripolymer protein combination and application thereof in preparation of bivalent RSV vaccines, and relates to the technical field of human vaccines. The vaccine is prepared by mixing an RSV-A subtype pre-F tripolymer protein and an RSV-B subtype pre-F tripolymer protein in equal mass, and matching with an aluminum hydroxide adjuvant with the concentration of 0.35 mg / 0.5 mL. A p27 fragment and a C-terminal esterification region of each of the two F proteins are deleted, the two F proteins are connected with F2 / F1 through GSGSGS, inter-chain disulfide bonds are introduced to S146C / N460C and A149C / Y458C sites respectively, intra-chain disulfide bonds are introduced to S155C / S290C sites respectively, and stable pre-F trimers are spontaneously assembled after secretion expression of CHO cells; the vaccine does not need to be freeze-dried, still keeps high purity and neutralizing epitope integrity after being stored at 37 DEG C for 28 days, can obviously induce neutralizing antibody and T cell immunity, and can be used for preventing various RSV-A / B infections.

The present disclosure relates to compositions and methods relating to interferon agonists and their engineered polypeptides. The engineered polypeptides have specificity to receptors in immune systems mediated by Type I interferon (IFN). The present disclosure also relates to methods and system for identifying surrogate agonists for cell surface receptors including dimeric and trimeric receptors.

The invention belongs to the technical field of coatings, and particularly relates to a two-component flexible road marking coating and a preparation method thereof. The two-component flexible road marking coating comprises a component A and a component B in a mass ratio of (2.5-3): 1, wherein the component A comprises 5-10 parts of bisphenol epoxy resin with the epoxy value of 0.4-0.55, 40-50 parts of polymer dihydric alcohol, 0.1-0.3 part of a catalyst, 10-15 parts of pigment, 15-20 parts of filler, 5-10 parts of an anti-skid material, 25-35 parts of internally mixed glass beads, 0.5-1 part of a dispersing agent, 0.5-1 part of a defoaming agent, 3-5 parts of a plasticizer and 30-60 parts of a solvent; the component B is a curing agent composed of modified isocyanate and an isocyanate tripolymer according to the mass ratio of (5-8): 1; the modified isocyanate is a compound which is prepared by carrying out hydrosilylation reaction on unsaturated isocyanate and siloxane with a silicon atom number of 2-4 and a hydrogen-containing terminal, and two ends of the modified isocyanate are isocyanato; the coating has excellent brittle fracture resistance, water resistance and wear resistance at low temperature.

The present application provides a vaccine comprising: 1) a peptide oligomer consisting of 2-8 copies of a bacteriophage T4 fibrin C-terminal polypeptide and one or more antigen proteins; or 2) a coding nucleic acid of item 1) wherein 2-8 copies of the bacteriophage T4 fibrin C-terminal polypeptides in the peptide oligomer are directly linked in series through chemical bonds or each of the bacteriophage T4 fibrin C-terminal polypeptides is linked to each other through a peptide linker, and the peptide oligomer and the antigen protein are directly connected through a chemical bond or connected through a peptide linker. In the bi-T4-virus antigen vaccine platform provided by the invention, the antigen can be subjected to multi-component change, that is, the antigen can form a trimer, so that the use flexibility of the vaccine platform is enhanced, the application range of the vaccine platform is widened, and the bi-T4 structure can enhance the formation of trimer protein and enhance the immune aging.

The invention provides an isocyanate curing agent composition and a preparation method thereof, and relates to the technical field of curing agents. The isocyanate curing agent composition is composed of an IPDI (isophorone diisocyanate) tripolymer prepolymer and a diisocyanate prepolymer, the weight content of NCO in the isocyanate curing agent composition is 5-10%, and the weight ratio of the IPDI tripolymer prepolymer to the diisocyanate prepolymer is (0.5-7): 1. The isocyanate curing agent composition provided by the invention has a relatively long pot life, and can improve the mechanical strength and durability of aspartic ester and other resins.

The invention relates to tumor-targeted trimeric polypeptides based on the homotrimerization region from collagen XV or XVIII and which contain domains capable of specifically binding to and activating receptors of the TNFR family of costimulatory receptors as well as to the uses thereof in the treatment of cancer.

The invention relates to germline-targeting designs, stabilization designs, and / or combinations thereof, of proteins designed with modified surfaces helpful for immunization regimens, other protein modifications and / or development of nanoparticles, methods of making and using the same, and to (a) germline-targeting priming or boosting / shepherding immunogens to initiate or guide maturation of VRC01-class responses (b) PCT64 / PG9-germline-targeting designs (c) BG18-germline-targeting designs or boosting / shepherding immunogens to initiate or guide maturation of BG18-like responses, and / or (d) trimer stabilization and presentation in a membrane-bound format.

The invention discloses a flame-retardant corrosion-resistant coating and a preparation method thereof, and relates to the technical field of fluorocarbon resin powder coatings. The flame-retardant corrosion-resistant coating comprises the following raw materials in parts by weight: 15-25 parts of fluorocarbon resin, 40-50 parts of epoxy resin, 3-5 parts of a curing agent, 5-7 parts of a reinforcing agent, 4-6 parts of a corrosion inhibitor, 6-8 parts of filler, 1-2 parts of a flatting agent and 0.5-1 part of benzoin. The preparation method comprises the following steps: reacting phosphonitrilic chloride trimer with p-hydroxybenzaldehyde to generate an intermediate 1, reacting the intermediate 1 with p-aminobenzoic acid to generate an intermediate 2, and reacting the intermediate 2 with 2-heptyl ethylene oxide to generate the reinforcing agent. The flame-retardant corrosion-resistant coating prepared by the invention has excellent mechanical properties, corrosion resistance, aging resistance and flame retardance.

A method and kit are provided for detecting anti-drug antibodies (ADAs) against self-assembling trimeric biologics, such as XPro1595, a pegylated variant of soluble human Tumor Necrosis Factor, in biological samples, particularly human serum. The method employs an Affinity Capture Elution-Protein A / G (ACE-AG) assay that overcomes non-specific reagent interactions inherent in conventional bridging and standard ACE assays due to the biologic's dynamic monomer exchange. The assay utilizes a two-plate system: a streptavidin-coated plate with biotinylated trimeric biologic captures ADAs, which are eluted, neutralized, and transferred to a protein A / G-coated plate for specific immunoglobulin capture and detection with a sulfo-tagged trimeric biologic via electrochemiluminescence. The kit includes pre-coated plates, labeled biologics, elution and neutralization solutions, assay buffer, confirmatory reagent, and instructions. The assay offers a robust, specific, and sensitive solution for immunogenicity testing of trimeric biologics.

The present application belongs to the field of veterinary drugs, and provides a recombinant protein S-Trimer of an infectious bronchitis virus antigen and a subunit vaccine therefor. Provided in the present application is a recombinant protein S-Trimer of an infectious bronchitis virus antigen, wherein the recombinant protein S-Trimer of the antigen has an amino acid sequence as set forth in SEQ ID No. 4. Also provided in the present application is an infectious bronchitis virus antigen. The infectious bronchitis virus antigen is of a trimer structure of the recombinant protein S-Trimer described above. The present application provides a subunit vaccine for an infectious bronchitis virus, wherein the subunit vaccine comprises a pharmaceutically acceptable carrier and an immunogenic amount of the infectious bronchitis virus antigen.

The application discloses an isocyanate polymerization catalyst and a preparation method thereof, and a preparation method of polyisocyanate, and a structural expression of the catalyst is as follows: the preparation method of the polyisocyanate is that, under an inert gas atmosphere, a polyisocyanate is used as a raw material to perform a self-polymerization reaction under the catalysis of the catalyst. The catalyst has a good catalysis effect, and the reaction temperature is stable, so that the catalyst is used for preparing an isocyanate trimer product with low viscosity and good storage stability.

The invention relates to a radionuclide labeled trimer probe 68Ga-TRAP-(FAPI) 3 targeting FAP and a preparation method thereof, Trap is used as a trivalent chelating platform, and three FAPI targeting units are covalently connected by clicking a chemical linking arm to form a trimer structure; the invention further relates to application of the 68Ga-TRAP-(FAPI) 3 probe in preparation of imaging agents or therapeutic drugs for diagnosing FAP positive diseases, experiments prove that the trimer probe is good in affinity and high in stability, and in cell experiments and animal experiments, the uptake rate of FAP positive cells is kept at a high level within 240 minutes; preclinical and preliminary clinical applications show that the tumor detection rate of the < 68 > Ga-TRAP-FAPI 3 in various cancer models is superior to that of < 18 > F-FDG, and compared with < 68 > Ga-FAPI-04, the < 68 > Ga-TRAP-FAPI 3 is clearer to display focuses (particularly tiny metastases), higher in uptake intensity and particularly outstanding in delayed imaging, and the < 68 > Ga-TRAP-FAPI 3 can be used for preparing a medicine for treating cancer. And a novel drug candidate with better performance is provided for precise diagnosis and staging of tumors and subsequent radionuclide targeted therapy.

The invention provides an FAPI trimer compound, a preparation method of the FAPI trimer compound, a PET developer based on the FAPI trimer compound and a preparation method of the developer. Amphipathic polyethylene glycol chains and trimeric structures in molecules of the FAPI trimer compound can improve in-vivo dynamic characteristics of the compound, and the in-vivo dynamic characteristics of the compound can be improved. The residence time in the tumor is prolonged; the PET imaging agent based on the FAPI trimer compound can improve the uptake and imaging effects of the PET imaging agent in tumors, and has good application prospects in PET imaging, preparation of drugs for diagnosing FAP-q expressed tumors and drugs for marking therapeutic nuclide (177Lu or 90Y) to treat the FAP-q expressed tumors in the future.

The invention relates to a base material of a self-lubricating sliding bearing as well as a preparation method and application of the base material. The base material comprises a component A, a component B and a component C, and the mass ratio of the component A to the component B to the component C is (0.4-0.8): (0.1-0.3): (0.1-0.3); wherein the component A is prepared from the following raw materials: vinyl cyclic siloxane, 1-thioglycerol and methacryloyl isocyanate; the component B is prepared from the following raw materials: a hexamethylene diisocyanate tripolymer and 2-hydroxy propyl methacrylate; and the component C is prepared from the following raw materials: a diphenylmethane diisocyanate tripolymer and 2-hydroxy propyl methacrylate. The compression strength and the compression modulus of the base material are both higher than those of commercially available resin, the bearing performance of the self-lubricating sliding bearing is greatly improved, meanwhile, the wear rate of the bearing is reduced through the high-crosslinking-degree resin, the gasket is more wear-resistant, and the service life of the bearing is prolonged.

The present invention discloses a kit for detecting African swine fever virus P30 antibodies and its application. The present invention obtains a recombinant African swine fever virus protein by serially connecting several regions with relatively strong immunogenicity in the amino acid sequence of the P30 protein, and then serially constructs a trimer of the recombinant protein. Foldon is then connected to the African swine fever virus protein serial trimer to construct a trimeric recombinant protein. Based on the trimeric recombinant protein, a kit for detecting African swine fever virus P30 antibodies is prepared. The kit has a higher sensitivity than many similar products on the market, with a sensitivity of up to 1:32,000, and is highly specific. Furthermore, the molecular weight of the trimeric recombinant protein has been increased by three times, further improving the stability and half-life of the kit. This is beneficial to the detection rate of African swine fever virus antibodies and has high application value. Furthermore, the kit is simple to prepare, has low production costs, and is easy to promote.

Herpesviridae glycoprotein B (gB) polypeptides comprising a modified ectodomain is stabilized in the prefusion state, enabling development of inhibitors and vaccines directed against these viral pathogens. Modifications to DI, DII, and DV subdomains are important to stabilization of gB in the prefusion state, and additional modifications result in a further improved stabilization of gB in the prefusion state. The gB can be derived from an Epstein Barr Virus (EBV), a Human Cytomegalovirus (CMV), a Human Herpesvirus 6 (HHV6), a Herpes Simplex Virus 1 (HSV1), or a Varicella Zoster Virus (VZV). Polypeptides, nucleic acid constructs, compositions, and methods of using same to elicit an immune response are described.

To provide a production method by which a fluoropolymer substantially free from dimers and trimers of a monomer constituting a polymer used in polymerization of a fluoromonomer can be produced.SOLUTION: A method for producing a fluoropolymer includes polymerizing a fluoromonomer in an aqueous medium in the presence of a polymer (1) to obtain a fluoropolymer, where the polymer (1) is a polymer of a monomer (1) represented by the general formula (1) defined by CF2=CF-R-CZ1Z2-A0 (where R is a linking group; Z1 and Z2 are each independently F or CF3; and A0 is an anionic group), in which the content of a dimer and a trimer of the monomer (1) is 1.0 mass% or less based on the polymer (1).SELECTED DRAWING: None