552 results about "Trimer" patented technology

This disclosure relates to dimeric immunotherapeutics that comprise two IgGs that are crosslinked with a disulfide bond. The two IgGs may be chimeras of two different heavy chains, in which one heavy chain includes a cysteine mutation that forms the disulfide bond, and the other heavy chain lacks the cysteine mutation. The presence of a cysteine mutation in only one of the heavy chains of an IgG avoids two disulfide bonds between the two IgGs, which increases the accessible orientations between the two crosslinked IgGs, and also avoids the formation of trimers and higher-order oligomers.

The invention provides an anti-aging flame-retardant epoxy resin coating and a preparation process thereof, and belongs to the technical field of coating preparation. The preparation process comprises the following steps: preparing the flame-retardant additive; preparing a coated modified black phosphorus nanosheet; preparing modified hollow glass beads; preparing the epoxy resin coating. The preparation method comprises the following steps: dissolving 3, 4-dihydroxy benzaldehyde and 2-amino-4-methylphenol in absolute ethyl alcohol, carrying out a heating reflux reaction, carrying out dehydration condensation on an aldehyde group in the 3, 4-dihydroxy benzaldehyde and an amino group in the 2-amino-4-methylphenol to generate an imine bond-containing intermediate, respectively dissolving the intermediate and phosphonitrilic chloride trimer, and carrying out a reaction to obtain the phosphonitrilic chloride trimer. The preparation method comprises the following steps: firstly, adding phosphonitrilic chloride into an intermediate, then mixing for reaction, enabling phenolic hydroxyl groups in the intermediate and phosphonitrilic chloride trimer to be subjected to nucleophilic substitution reaction to generate the flame-retardant additive containing phosphorus and a Schiff base structure, and the flame-retardant additive has relatively good compatibility with an epoxy resin matrix, is not easy to separate out and can effectively improve the flame-retardant property of the epoxy resin coating.

The invention relates to the field of cables, and discloses a modified polyvinyl chloride composite material for cables and a preparation method thereof.The composite material comprises low-density polyethylene, polyvinyl chloride, ethylene-vinyl acetate copolymer, nitrile rubber, modified graphene oxide, modified nano calcium carbonate and auxiliaries; the modified graphene oxide is prepared by reacting acylating chlorination of graphene oxide with an anti-aging derivative, the anti-aging derivative is prepared by reducing a benzoxazole-containing intermediate generated by a reaction of 2-amino-4-bromophenol and 4-cyanobenzaldehyde to obtain an aminated benzoxazole-containing intermediate, then the aminated benzoxazole-containing intermediate reacts with p-phenylenediamine, and then the product reacts with 3-(3, 4-dichlorophenyl)-1, 2, 3-triazole-3-one to obtain the anti-aging graphene oxide. 2, 5-di-tert-butyl-4-hydroxyphenyl) propionyl chloride is subjected to a reaction to prepare the product. And the modified nano calcium carbonate is prepared by reacting phosphonitrilic chloride trimer with an amino silane coupling agent and 2-amino benzoxazole-5-borate and then grafting on the surface of the nano calcium carbonate. The composite material prepared by the invention has excellent flame retardance, heat resistance and long-acting oxidation resistance.

The invention discloses an antistatic polyester fiber and a preparation method thereof, and relates to the field of fibers. When the antistatic polyester fiber is prepared, N, N-dimethylethanolamine reacts with allyl chloride, and then dichloro phthalocyanine silicon is grafted to obtain modified silicon phthalocyanine; polymerizing terephthalic acid, itaconic acid and ethylene glycol, and reacting with fluorescein isothiocyanate and modified silicon phthalocyanine in sequence to obtain modified polyester chips; the preparation method comprises the following steps: reacting phosphonitrilic chloride trimer with 3-hydroxy-5-nitropyridazine, and reducing to obtain a flame retardant; and carrying out melt spinning on the modified polyester chips and a flame retardant, and reacting to obtain the antistatic polyester fiber. The antistatic polyester fiber prepared by the invention has excellent antistatic property, antibacterial property, flame retardance and mechanical property.

The invention discloses a novel coronavirus neutralizing antibody, a detection kit and application of the novel coronavirus neutralizing antibody. The amino acid sequence of a heavy chain variable region of the neutralizing antibody is shown as SEQ ID No.1, and the amino acid sequence of a light chain variable region of the neutralizing antibody is shown as SEQ ID No.2. The antibody with mature affinity is screened through bioinformatics analysis of a single B cell, the antibody screening process is optimized by combining single cell RNA sequencing, VDJ rearrangement analysis and somatic cell hypermutation research, blindness of a traditional method is avoided, and the accuracy and effectiveness of antibody screening are improved. According to the neutralizing antibody GR12 provided by the invention, a heavy chain variable region and a light chain variable region of the neutralizing antibody GR12 can be specifically combined with an RBD structural domain of the SARS-CoV-2 and an S-Trimer structural domain of an Omicro variant, so that a broad-spectrum neutralizing effect on the SARS-CoV-2 virus and the variant thereof is realized. The binding activity of the antibody GR12 to S-Trimer and RBD under 2-fold and 300-fold dilution conditions is obviously superior to that of other antibodies, which indicates that the antibody GR12 has high affinity and dilution stability and is suitable for clinical large-dose administration.

The invention discloses a CD40L-IL-2 fusion protein, preparation thereof and application of the CD40L-IL-2 fusion protein in preparation of drugs for treating tumors. The fusion protein comprises a CD40L trimer or a variant thereof, and a cytokine IL-2 or a variant thereof; the CD40L trimer comprises three CD40L monomers which are connected with one another. The fusion protein provided by the invention can synergistically activate APC-dependent antigen presentation and T cell-mediated immune killing to form closed-loop anti-tumor immune response of antigen presentation-immune initiation-effect amplification, and an effective strategy is provided for tumor treatment.

The invention discloses a novel coronavirus neutralizing antibody, a detection kit and application of the novel coronavirus neutralizing antibody. The amino acid sequence of a heavy chain variable region of the neutralizing antibody is shown as SEQ ID No.1, and the amino acid sequence of a light chain variable region of the neutralizing antibody is shown as SEQ ID No.2. The antibody with mature affinity is screened through bioinformatics analysis of a single B cell, the antibody screening process is optimized by combining single cell RNA sequencing, VDJ rearrangement analysis and somatic cell hypermutation research, blindness of a traditional method is avoided, and the accuracy and effectiveness of antibody screening are improved. According to the neutralizing antibody GR75 provided by the invention, a heavy chain variable region and a light chain variable region of the neutralizing antibody GR75 can be specifically combined with an RBD structural domain of the SARS-CoV-2 and an S-Trimer structural domain of an Omicro variant, so that a broad-spectrum neutralizing effect on the SARS-CoV-2 virus and the variant thereof is realized. The binding activity of the antibody GR75 to S-Trimer and RBD under 2-fold and 300-fold dilution conditions is obviously superior to that of other antibodies, which indicates that the antibody GR75 has high affinity and dilution stability and is suitable for clinical large-dose administration.

The invention discloses a novel coronavirus neutralizing antibody GR46, a detection kit and application of the novel coronavirus neutralizing antibody GR46. The amino acid sequence of a heavy chain variable region of the neutralizing antibody is shown as SEQ ID No.1, and the amino acid sequence of a light chain variable region of the neutralizing antibody is shown as SEQ ID No.2. The antibody with mature affinity is screened through bioinformatics analysis of a single B cell, the antibody screening process is optimized by combining single cell RNA sequencing, VDJ rearrangement analysis and somatic cell hypermutation research, blindness of a traditional method is avoided, and the accuracy and effectiveness of antibody screening are improved. According to the neutralizing antibody GR46 provided by the invention, a heavy chain variable region and a light chain variable region of the neutralizing antibody GR46 can be specifically combined with an RBD structural domain of the SARS-CoV-2 and an S-Trimer structural domain of an Omicro variant, so that a broad-spectrum neutralizing effect on the SARS-CoV-2 virus and the variant thereof is realized. The binding activity of the antibody GR46 to S-Trimer and RBD under 2-time and 300-time dilution conditions is obviously superior to that of other antibodies, which indicates that the antibody GR46 has high affinity and dilution stability and is suitable for clinical large-dose administration.

The invention belongs to the field of veterinary drugs, and relates to an avian infectious bronchitis virus antigen recombinant protein S-Trimer and a subunit vaccine thereof. The invention provides an avian infectious bronchitis virus antigen recombinant protein S-Trimer. The amino acid sequence of the antigen recombinant protein S-Trimer is as shown in SEQ ID No. 4. The invention provides an infectious bronchitis virus antigen. The infectious bronchitis virus antigen is of a trimer structure of the recombinant protein S-Trimer. The invention provides a chicken infectious bronchitis virus subunit vaccine. The subunit vaccine comprises a pharmaceutically acceptable carrier and an immune dose of the chicken infectious bronchitis virus antigen. The vaccine is high in safety, good in immunogenicity and stable in batches, and can provide complete protection for attacking the infectious bronchitis virus.

The invention discloses an antigen fusion protein and application thereof in preparation of a live vaccine for preventing infectious bursal disease. Specifically disclosed are antigen fusion proteins comprising a VP2 protein, a trimer tag, and a C3d protein. The invention also discloses recombinant Eimeria heap constructed by using the antigen fusion protein and application of the recombinant Eimeria heap in preparation of IBDV live vaccines. The oocyst of the recombinant Eimeria heap can effectively induce humoral immune response aiming at IBDV as a vaccine active ingredient, the level of an induced antibody is obviously higher than that of an insect strain expressed by a single VP2 antigen, viruses can be more effectively neutralized, the immune protection efficacy is improved, the safety is high, and the oocyst has the potential of serving as a live vector genetic engineering vaccine. The IBDV live vaccine can be orally taken through drinking water or feed, vaccination is simple, large-scale immunization of chicken flocks can be achieved, and the IBDV live vaccine is economical and efficient and has a wide prospect of being applied to prevention and control of the IBDV of the chicken flocks.

The invention discloses an indoor flame-retardant insulated flexible wire and a preparation method thereof, belongs to the technical field of flexible wires, and is used for solving the technical problem that the insulating property and flame-retardant property of an indoor flexible wire in the prior art need to be further improved. The cable comprises a wire layer formed by twisting a plurality of copper wires, an insulating layer and an outer sheath layer, a modified aminating agent taking a siloxane chain segment as a main body is prepared, polyvinyl chloride is aminated and hybridized with a space chain segment of the polyvinyl chloride at the same time, after phosphorus-hydrogen addition and surface modification, the hybridized polyvinyl chloride is obtained, and through sodium hypophosphite and 1, 2, 4-trimethyl-1, 3-pentanediol, the flame retardant is added to the polyvinyl chloride, so that the cable is formed. The preparation method comprises the following steps: carrying out phosphorus-hydrogen addition on 2, 3-dihydroxystyrene to obtain a modified cross-linking agent, forming a spherical structure by the modified cross-linking agent and phosphonitrilic chloride trimer under triethylamine and ultrasonic conditions, carrying out surface modification to obtain flame-retardant microspheres, and carrying out melt extrusion on the modified cross-linking agent and phosphonitrilic chloride trimer as main materials and auxiliary materials of an outer sheath layer to finally prepare the flame-retardant insulated flexible wire.

The invention relates to the technical field of polyurethane compositions, in particular to a chlorotrifluoropropene-based high-temperature-resistant polyurethane composition and a preparation process thereof, and the chlorotrifluoropropene-based high-temperature-resistant polyurethane composition comprises the following raw materials: hydroxylated chlorotrifluoropropene, modified hexamethylene diisocyanate tripolymer, phthalic anhydride polyester polyol, nano silicon carbide whiskers and polyimide microspheres. A fluorine element is introduced into hydroxylated chlorotrifluoropropene, so that the thermal decomposition temperature of the material is remarkably increased, and thermal movement and oxidative degradation of a molecular chain at high temperature are inhibited; meanwhile, a rigid benzene ring structure of bisphenol A dihydroxyethyl ether improves the regularity of a molecular chain and reduces high-temperature softening deformation. The modified hexamethylene diisocyanate tripolymer forms a stable siloxane cross-linked network, so that the thermal stability of the material is further enhanced; in addition, an isocyanate group reacts with hydroxyl to generate a high-density carbamate crosslinking point, so that the regularity of a hard segment is enhanced, and the thermal stability of the material is improved.

The invention discloses a novel plasmid vector capable of increasing CHO foreign protein expression quantity and construction and application thereof, and belongs to the technical field of gene engineering. The plasmid vector pCHO6GS belongs to a brand-new plasmid system, and the expression quantity of a common monoclonal antibody and recombinant protein (such as H5HA trimer) difficult to express in CHO can be increased by at least more than one time, so that the expression quantity of foreign protein is increased beyond expectation through the novel plasmid vector; and the method also plays an extremely important role in reducing cost and improving efficiency of various protein products taking CHO as a cell factory.

To provide a heat transfer fluid having a relatively high boiling point.SOLUTION: The present disclosure provides a composition for a heat transfer fluid, which contains hexafluoropropene trimers having specific three structures, (I), (II), and (III), and in which a compound having the structure (I) is contained in an amount of 10 mass% or more and less than 85 mass% relative to the total amount of the hexafluoropropene trimers having the three structures.SELECTED DRAWING: None

The invention discloses a reconstructed respiratory syncytial virus F protein trimer, relates to the technical field of genetic engineering vaccines, and designs an RSV F protein trimer, a monomer of the trimer does not contain a p27 sequence, and a furin enzyme digestion peptide fragment aa110-aa136 is deleted; the F2 structural domain and the F1 structural domain are connected through a GS connexon; the invention also designs point mutation combinations, and the point mutation combinations can form an inter-chain disulfide bond, so that covalent connection of the trimer is realized, and the problem of depolymerization of the trimer is fundamentally solved; the trimer is stabilized in a pre-F state, a post-F sample structure does not exist, and an ELISA result shows that a neutralizing epitope is completely reserved; the trimer antigen structure is challenged by adopting a thermal acceleration experiment method, and a result shows that the purity of the trimer antigen reaches 95% or above after the trimer antigen is placed at 37 DEG C for 28 days, and the trimer antigen is very good in stability; when the trimer is used for preparing the vaccine, the standard required by laws and regulations can be met without freeze-drying.

The present invention relates to the technical field of biomedicine. Specifically provided are a respiratory syncytial virus (RSV) Pre-F recombinant protein nanoparticle vaccine and a preparation method therefor. By performing amino acid mutation on an RSV Pre-F protein, and performing fusion expression on the mutated Pre-F protein and ferritin particles in eukaryotic cells, PreF-Ferritin fusion protein nanoparticles, in which eight Pre-F protein trimers are densely displayed on the surface of each ferritin particle, are obtained. By stabilizing and exposing antigen epitopes required to be displayed and disrupting or masking unrequired antigen epitopes, the PreF-Ferritin fusion protein nanoparticles effectively improve the immunogenicity and the production stability of the antigen. Experiments show that: when the PreF-Ferritin fusion protein is injected into mice, high-titer protective sera can be acquired, and the mice sera can yield relatively high neutralizing titers against RSV.

The invention discloses a star-shaped tripolymer type non-fullerene acceptor material as well as preparation and application thereof. The star-shaped tripolymer type non-fullerene acceptor material has a structure as shown in the following formula: # imgabs0 #. According to the invention, a star-shaped trimer type oligomer material connected with a quinoxaline development center with a derivative site in the middle is adopted, so that the defect that the PCE of the current oligomer type acceptor material is influenced due to oligomerization based on a halogen substitution site of tail-end isatin is overcome; compared with a traditional small molecule material (oligomer type acceptor material), the material has higher open-circuit voltage (Voc), the photoelectric conversion efficiency is excellent, the illumination stability is good, and when the material is applied to a solar cell, the photoelectric conversion efficiency and stability of the cell can be remarkably improved.

The present invention relates to combination therapies employing tumor targeted anti-CEA / CD3 bispecific antibodies and / or agents blocking PD-L1 / PD-1 interaction in combination with 4-1BB (CD137) agonists, in particular 4-1BBL trimer containing antigen binding molecules that also target FAP, the use of these combination therapies for the treatment of cancer and methods of using the combination therapies.

The present invention relates to a conjugate for inhibiting viral replication, a preparation method therefor, and use thereof. The conjugate comprises a dimer or trimer of a portion that inhibits influenza virus neuraminidase (e.g. zanamivir, peramivir, oseltamivir, or an analog thereof), the dimer or trimer being conjugated to an Fc domain monomer, an Fc domain, an Fc-binding peptide, an albumin, or an albumin-binding peptide. The conjugate can be used to treat viral infections (e.g., influenza virus infections).

The invention relates to the technical field of adhesives, and provides a polyurethane adhesive and a preparation method and a use method thereof.The polyurethane adhesive comprises a first component and a second component, the first component is prepared from a polyurethane prepolymer, isocyanate, pigment filler and a thixotropic agent; the second component is prepared from the following raw materials: polyol, a water removal agent, a pigment filler, a first catalyst and a second catalyst; the polyurethane prepolymer is obtained through reaction of a low-activity isocyanate monomer and a chain extender, the low-activity isocyanate monomer is selected from at least one of isophorone diisocyanate, hexamethylene diisocyanate, dicyclohexylmethane diisocyanate and a hexamethylene diisocyanate tripolymer, and the chain extender is selected from at least one of isophorone diisocyanate, hexamethylene diisocyanate, dicyclohexylmethane diisocyanate and hexamethylene diisocyanate tripolymer. The first catalyst is selected from at least one of an organic tin catalyst and an organic bismuth catalyst, and the second catalyst is selected from at least one of amine catalysts.

A process for preparing middle distillates from an olefinic feedstock, bya) oligomerization fed with the olefinic feedstock, a first recycle and a second recycle, and operated in the presence of at least one oligomerization catalyst, to produce a reaction effluent containing dimers, trimers and oligomers;b) fractionating the reaction effluent into:a light fraction containing at least part of the unconverted olefinic feedstock;an intermediate fraction containing at least part of the dimers and trimers; anda heavy fraction containing the oligomers;c) recycling by the preparation of a first recycle containing at least part of the light fraction; and a second recycle containing at least part of the intermediate fraction; and transfer of the first recycle and the second recycle to step a);d) hydrogenating at least part of the heavy fraction.

The invention discloses a polyurethane repairing material for underground engineering concrete, and relates to the technical field of engineering leakage treatment. A component A is a polyether polyol system containing a silane coupling agent and active inorganic powder, a component B is an emulsion of a modified MDI prepolymer-aliphatic isocyanurate tripolymer and an alkaline activator, the two components are instantaneously converged in a three-cavity grouting gun and a constant-temperature air three-runner according to the mass ratio of 0.8-1.2: 1, gel is formed in the environment of-10 DEG C to 50 DEG C for 90 s, and water is stopped in 10 min. The micro-foaming expansion rate is 2-4 times, geopolymers are synchronously generated, organic-inorganic dual-curing interlocking is achieved, the closed porosity is larger than or equal to 95%, and the compression strength is gt; the permeability coefficient is less than or equal to 10-m / s. Silane chemical bridging enables the bonding strength to be higher than the splitting strength of the base material.

The invention discloses an RSV pre-F tripolymer protein combination and application thereof in preparation of bivalent RSV vaccines, and relates to the technical field of human vaccines. The vaccine is prepared by mixing an RSV-A subtype pre-F tripolymer protein and an RSV-B subtype pre-F tripolymer protein in equal mass, and matching with an aluminum hydroxide adjuvant with the concentration of 0.35 mg / 0.5 mL. A p27 fragment and a C-terminal esterification region of each of the two F proteins are deleted, the two F proteins are connected with F2 / F1 through GSGSGS, inter-chain disulfide bonds are introduced to S146C / N460C and A149C / Y458C sites respectively, intra-chain disulfide bonds are introduced to S155C / S290C sites respectively, and stable pre-F trimers are spontaneously assembled after secretion expression of CHO cells; the vaccine does not need to be freeze-dried, still keeps high purity and neutralizing epitope integrity after being stored at 37 DEG C for 28 days, can obviously induce neutralizing antibody and T cell immunity, and can be used for preventing various RSV-A / B infections.

The present disclosure relates to compositions and methods relating to interferon agonists and their engineered polypeptides. The engineered polypeptides have specificity to receptors in immune systems mediated by Type I interferon (IFN). The present disclosure also relates to methods and system for identifying surrogate agonists for cell surface receptors including dimeric and trimeric receptors.

The invention belongs to the technical field of coatings, and particularly relates to a two-component flexible road marking coating and a preparation method thereof. The two-component flexible road marking coating comprises a component A and a component B in a mass ratio of (2.5-3): 1, wherein the component A comprises 5-10 parts of bisphenol epoxy resin with the epoxy value of 0.4-0.55, 40-50 parts of polymer dihydric alcohol, 0.1-0.3 part of a catalyst, 10-15 parts of pigment, 15-20 parts of filler, 5-10 parts of an anti-skid material, 25-35 parts of internally mixed glass beads, 0.5-1 part of a dispersing agent, 0.5-1 part of a defoaming agent, 3-5 parts of a plasticizer and 30-60 parts of a solvent; the component B is a curing agent composed of modified isocyanate and an isocyanate tripolymer according to the mass ratio of (5-8): 1; the modified isocyanate is a compound which is prepared by carrying out hydrosilylation reaction on unsaturated isocyanate and siloxane with a silicon atom number of 2-4 and a hydrogen-containing terminal, and two ends of the modified isocyanate are isocyanato; the coating has excellent brittle fracture resistance, water resistance and wear resistance at low temperature.

Provided herein are self-assembling collagen-like peptide (CLP) comprising a plurality of amino acid trimer repeats having the amino acid sequence POG, wherein P is a proline, O is an hydroxyproline or a cysteine, and G is a glycine or a valine. Also provided herein are compositions, scaffolds, photoactivatable hydrogel precursors and hydrogels comprising the CLP and a multi-arm polyethylene glycol (PEG) polymer. Methods of preparing photoactivatable hydrogel precursors and hydrogels are also described. Methods of treating a wound, of treating a corneal disease, of treating a myocardial infarct, of promoting cell growth and of treating cellular degeneration using the compositions, scaffolds, photoactivatable hydrogel precursors and / or hydrogels are also described. Also provided is a device for delivering a peptide-based material. The device comprises an on / off button or switch for initiating and stopping delivery of the peptide-based material in the subject; a chamber for containing the peptide-based material; an aperture through which the peptide-based material exits the chamber, wherein the aperture is adapted for receiving a nozzle adapter; a camera port for securing a camera thereto; and a digital control display.

The invention relates to the technical field of coatings, in particular to a bio-based polyurethane waterproof coating and a preparation method thereof. The coating is prepared from organic fluorine-silicon cross-linked castor oil, dibutyltin dilaurate, an HDI tripolymer, polytetrahydrofuran ether glycol, 1, 4-butanediol, a filler, a plasticizer and an ultraviolet light absorber, and the organic fluorine-silicon cross-linked castor oil is obtained by silanization modification of methyl ricinoleate and hydrolysis cross-linking of fluorine-containing silane. The preparation method is simple, and the material has excellent performance, is suitable for the fields of building waterproofing, weather-resistant coatings and the like, and has wide application potential and market value.

The invention relates to the technical field of veterinary biological products, and particularly provides a porcine epidemic diarrhea virus S protein and related products. The amino acid sequence of the porcine epidemic diarrhea virus S protein fragment is as shown in SEQ ID NO.4. The trimer S protein is constructed by fusing a trimer tag at the C terminal of a protective antigen in a truncated form of the porcine epidemic diarrhea virus S protein. After expression and purification, immunogenicity is judged through animal evaluation, and it is verified that the vaccine prepared from the trimer S protein has the advantages of being high in safety, good in immunogenicity, stable in batches, capable of inducing piglets to generate good immunoreaction and the like, and porcine epidemic diarrhea can be effectively treated or prevented.

The present application provides a vaccine comprising: 1) a peptide oligomer consisting of 2-8 copies of a bacteriophage T4 fibrin C-terminal polypeptide and one or more antigen proteins; or 2) a coding nucleic acid of item 1) wherein 2-8 copies of the bacteriophage T4 fibrin C-terminal polypeptides in the peptide oligomer are directly linked in series through chemical bonds or each of the bacteriophage T4 fibrin C-terminal polypeptides is linked to each other through a peptide linker, and the peptide oligomer and the antigen protein are directly connected through a chemical bond or connected through a peptide linker. In the bi-T4-virus antigen vaccine platform provided by the invention, the antigen can be subjected to multi-component change, that is, the antigen can form a trimer, so that the use flexibility of the vaccine platform is enhanced, the application range of the vaccine platform is widened, and the bi-T4 structure can enhance the formation of trimer protein and enhance the immune aging.

The invention provides an isocyanate curing agent composition and a preparation method thereof, and relates to the technical field of curing agents. The isocyanate curing agent composition is composed of an IPDI (isophorone diisocyanate) tripolymer prepolymer and a diisocyanate prepolymer, the weight content of NCO in the isocyanate curing agent composition is 5-10%, and the weight ratio of the IPDI tripolymer prepolymer to the diisocyanate prepolymer is (0.5-7): 1. The isocyanate curing agent composition provided by the invention has a relatively long pot life, and can improve the mechanical strength and durability of aspartic ester and other resins.