53 results about "Collagen scaffold" patented technology

Shape-stabilized collagen scaffolds and methods of obtaining such scaffolds are disclosed. Stroma can be harvested, for example, from human or porcine corneal stroma and shaped during excision or in a separate step after excision. Following shaping (and preferably decellularization), the excised stroma portion is subject to pressure, force or vacuum to reduce fluid content and then irradiated or otherwise treated to induce crosslinking of collagen chains or fibrils. In one embodiment, the scaffold can be compacted by removing some or all of the water from the scaffold, and rehydrating the scaffold in a controlled manner (e.g., in a mold or other confining space) such that the scaffold takes a desired compacted shape; and then crosslinking at least a portion of the scaffold to mechanically strengthen it and inhibit subsequent swelling. Various sources of energy can be employed to induce crosslinking of collagen including, for example, ultraviolet (UV) radiation. The scaffolds can also be selectively densified or patterned. The invention is particularly useful in forming stable lenticules of enhanced stiffness and sufficient optical clarity for intracorneal implantation in additive ocular surgery.

The invention provides a preparation method of a mineralized collagen scaffold material with a bionic structure, and belongs to the field of biomedical engineering.The preparation method comprises the steps that an amorphous calcium phosphate solution stabilized by polyacrylic acid is prepared, and the molar ratio of Ca to P in the amorphous calcium phosphate solution is 1.67: 1; preparing a collagen solution containing noradrenaline; carrying out isoelectric focusing treatment on the collagen solution under a constant voltage to form a dense membrane, and stretching the dense membrane to generate 120-200% deformation, so as to obtain a collagen membrane with a bionic structure; and soaking the collagen membrane in the amorphous calcium phosphate solution, and then performing freeze drying to obtain the noradrenaline-mineralized collagen scaffold material. The mineralized collagen scaffold material prepared by the method has excellent biocompatibility, osteogenesis inductivity and sufficient mechanical strength, can effectively promote bone tissue defect repair, and has a good application prospect in the field of bone tissue engineering. The invention further provides the mineralized collagen scaffold material with the bionic structure and application of the mineralized collagen scaffold material.

The invention relates to a collagen scaffold with a fixed-axis orientation structure and a preparation method and application thereof, and the preparation method comprises the following steps: (1) dropwise adding a soluble phosphate solution into a collagen-soluble calcium salt mixed solution, and adjusting the pH value with ammonia water; (2) injecting the obtained mixed solution into a mold of which the bottom is provided with a heat-conducting bottom plate; vacuumizing is conducted, heat insulation treatment is conducted on the part, except for the heat conduction bottom plate, of the mold, and then the mold is frozen and formed at the temperature ranging from-20 DEG C to-180 DEG C; and (3) freeze drying. According to the preparation method, heat insulation treatment is conducted on the part, except for a heat conduction bottom plate, of a mold, only the heat conduction bottom plate is left for heat exchange with the external environment, during freezing, low temperature is gradually transmitted upwards from the heat conduction bottom plate to form a temperature gradient, ice crystals of an oriented structure are formed, and then the ice crystals are removed through freeze drying. The interior of the scaffold can be provided with a structure which is similar to natural bones and is arranged in a Harvard system, so that osteoblast proliferation is facilitated, and good conditions are created for growth of cells and blood vessels.

The invention aims to realize self-adhesion fixation of a cartilage repair scaffold, and provides a collagen scaffold preparation method capable of controllably removing terminal peptide, which comprises the following steps: S1, cleaning and shearing a collagen raw material to obtain a granular collagen raw material; s2, dehydrating, centrifuging and dispersing the granular collagen material to obtain a dispersed collagen material; s3, performing enzymolysis treatment on the dispersed collagen material to obtain a collagen solution; step S4, preparing a collagen scaffold by adopting a collagen solution, in the step S3, the removal rate of terminal peptides in the collagen solution is controlled to be 60%-80%, and the step S3 comprises the following steps: adding water, glacial acetic acid and pepsase into the dispersed collagen material according to the mass ratio of the dispersed collagen material to the water to the glacial acetic acid to the pepsase being 1: (5-25): (0.3-1.0): (0.05-1.5); keeping the temperature at 4-8 DEG C for 48-120 hours, and stirring for multiple times during the keeping period; and performing low-temperature gradient dialysis at the temperature of 4-8 DEG C to remove enzyme solution residues, thereby obtaining the collagen solution. The invention also provides the collagen scaffold prepared by the method and application of the collagen scaffold.

A method of treating injured skeletal muscle tissue generally includes applying to injured skeletal muscle tissue a therapeutic composition that includes a purified exosome product (PEP) and a pharmaceutically acceptable carrier. In one or more embodiments, the method includes applying from 1×1011 PEP exosomes to 1×1013 PEP exosomes to the injured skeletal muscle. In one or more embodiments, the therapeutic composition further includes a supportive matrix such as a collagen scaffold, a tissue sealant, fibrin glue, or a hydrogel.

The invention discloses a method for screening an active matter for protecting skin cell telomeres, and relates to the technical field of biotechnology and cosmetic development, and the method is technically characterized by comprising the following steps: a) providing stem cells derived from an exosome which carries at least one microRNA for regulating and controlling a telomere protection factor CTC1; b) acting a candidate active matter on the stem cells, and separating to obtain a treated exosome; the technical effects are as follows: the combination of miR-150-5p and miR-181c is delivered through the exosome, the dual-miRNA coordinated regulation is realized, the limitation of the traditional single-target detection is broken through, the CD63 / CD81 protein on the membrane surface of the exosome can be specifically combined with the skin cell membrane, and the Franz diffusion experiment verifies that the micro-fluidic chip integrates the collagen scaffold and the dynamic shear force.

A biologic matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized, suspension-dried mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more of its outer surfaces. The pores are created by forcing a gas through the vascular pathways of the mammalian organ or tissue while it is in a suspended position. This enables the gas to inflate the vascular pathways in all directions without restriction. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 10 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 15 times larger or more than the non-porous scaffold of equivalent outer dimensions.

The invention provides a double-layer collagen scaffold repair membrane as well as a preparation method and application thereof, and the preparation method comprises the following steps: extracting collagen from animal fur, and emulsifying to obtain a collagen emulsion; adding a cross-linking agent into the collagen emulsion, and emulsifying to obtain a cross-linked protein emulsion; pouring the cross-linked protein emulsion into a mold, and freeze-drying to obtain a cross-linked protein layer; and pouring the collagen emulsion on the cross-linked protein layer, and freeze-drying to obtain the double-layer collagen scaffold repair membrane. The repairing membrane is excellent in structure, the cross-linked protein layer provides mechanical strength and supporting performance, the collagen layer can be rapidly dissolved and attached to the contact surface, and oxygen exchange and metabolite discharge of the wound surface are facilitated; the collagen is high in purity, low in antigenicity and high in safety, any toxic and harmful chemical reagents and preservatives are not added, allergy or inflammatory reaction is avoided, and the collagen has good application prospects in the fields of medical treatment, medical beauty, beauty, skin care and the like.

The invention discloses a high-strength self-lubricating three-layer nested medicine-carrying hollow cervical cerclage band with an auxiliary miscarriage prevention function and a preparation method of the cerclage band, and belongs to the technical field of medical instruments. Degradable polyester monofilaments are woven into a hollow round rope, and the hollow round rope and a medical curved needle are integrated; then carrying out polydopamine-polyethylene glycol double-layer modification on a copper-based metal organic framework (Cu-MOF), and loading active molecules; then mixing the drug-loaded Cu-MOF with collagen natural polymer sol, and uniformly filling the gel into the inner cavity of the round rope and forming a three-dimensional bracket through synchronous drawing type micro-perfusion and light-enzyme synergistic cross-linking; then constructing a transient hydrated lubricating layer on the surface; and finally carrying out ethylene oxide final sterilization to obtain a finished product. The cervical cerclage band device provided by the invention simultaneously provides degradable mechanical support, collagen scaffold induction and long-acting slow release regulation, has a low-friction implantation characteristic, and can be well used for preventing or treating middle and late abortion and premature delivery caused by cervical insufficiency.

A sterilized scaffold comprising loaded cells and a method for preparing a sterilized scaffold for medical use, the method comprising the steps of:i) Loading collagen to a fiber mesh containing fibers of polylactide polymer or copolymer (commonly denoted PLA) to obtain a PLA-collagen scaffold,ii) Drying the PLA-collagen scaffold obtained from step i),iii) Sterilizing the PLA-collagen scaffold obtained from the drying step ii) to obtain the sterilized scaffold, and optionallyiv) Loading cells such as pre-proliferated cells into the scaffold.The sterilized scaffold obtained has improved biomechanical properties compared with an unsterilized scaffold.

The invention relates to the field of bone tissue engineering and the field of biomedical materials, in particular to application of an ultrasonic response piezoelectric collagen scaffold. According to the invention, full-thickness pigskin is used as a raw material, hair and subcutaneous adipose tissue are removed, and then decellularization is carried out so as to obtain the membrane-like scaffold. The membrane-like scaffold retains a complete three-stage structure of natural collagen, which is a source of the natural collagen having a piezoelectric effect; meanwhile, a piezoelectric signal detection result shows that periodic piezoelectric signals appear on the membrane-shaped stent, and then the collagen stent provided by the invention is proved to have an intrinsic piezoelectric effect. Experimental results show that mesenchymal stem cells can be attached to the surface of the collagen scaffold and can promote repair and regeneration of bone tissues, and in addition, rat subcutaneous ectopic bone regeneration experiments prove that the collagen scaffold provided by the invention has a good bone regeneration promoting effect under ultrasonic stimulation.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.

The application aims to realize self-adhesive fixation of cartilage repair scaffolds, and provides a collagen scaffold preparation method with controllable removal of terminal peptides, comprising the following steps: S1, cleaning and shearing collagen raw materials to obtain granular collagen materials; S2, dehydrating, centrifuging and dispersing the granular collagen materials to obtain dispersed collagen materials; S3, performing enzymatic treatment on the dispersed collagen materials to obtain a collagen solution; and S4, preparing a collagen scaffold by using the collagen solution, wherein the removal rate of terminal peptides in the collagen solution is controlled at 60%-80% in step S3, which comprises: adding water, glacial acetic acid and pepsin into the dispersed collagen materials according to the mass ratio of dispersed collagen materials:water:glacial acetic acid:pepsin=1:(5-25):(0.3-1.0):(0.05-1.5); keeping at a temperature of 4-8 DEG C for 48 h-120 h, and stirring several times during the keeping period; and performing low-temperature gradient dialysis under a temperature condition of 4-8 DEG C to remove residual enzyme solution, thereby obtaining the collagen solution. The application further provides the collagen scaffold prepared by the method and application thereof.

A sterilized PLA-collagen scaffold comprising animal collagen and a method for preparing a sterilized scaffold for medical use, the method comprising the steps of:i) Loading animal collagen to a fiber mesh containing fibers of polylactide polymer or copolymer (commonly denoted PLA) to obtain a PLA-collagen scaffold,ii) Drying the PLA-collagen scaffold obtained from step i),iii) Sterilizing the PLA-collagen scaffold obtained from the drying step ii) to obtain the sterilized scaffold.The sterilized scaffold obtained has improved biomechanical and physical properties compared with an unsterilized scaffold.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.

The application provides a preparation method of a mineralized collagen scaffold medical material prepared by a polymer-induced liquid precursor, and phosphine polyethyleneimine and polyacrylic acid are used in a high-concentration calcium phosphate solution to prepare a polymer-induced liquid precursor; collagen fiber scaffolds are immersed in the polymer-induced liquid precursor to be mineralized to obtain the mineralized collagen scaffold medical material; the polymer-induced liquid precursor comprises the following components in percentage by mass: 1-3% of phosphine polyethyleneimine, 4-6% of polyacrylic acid, 0.5-1% of calcium chloride, 2.5-3% of disodium hydrogen phosphate and the balance of water. The PILP in the application can penetrate into the inside of the collagen scaffold to be mineralized, the mineralization effect of the collagen scaffold is enhanced, the biocompatibility and osteogenic activity of the collagen scaffold are improved, and the application prospect of the PILP in the field of bone repair materials is wide.

A multispectral photonic imaging system and associated methods are disclosed for classifying wound tissue and generating customized therapeutic wound dressings. The system employs wavelength-specific optical sources and integrated image sensors to capture multispectral reflectance data from a wound, enabling computational classification of distinct tissue types such as granulation, slough, and necrosis. A three-dimensional (3D) digital model of the wound is constructed using spatial data from depth sensors. The classified 3D wound model facilitates automated design of patient-specific dressings, featuring heterogeneous regions tailored with therapeutic compounds like antimicrobial agents, analgesics, collagen scaffolds, and hydrogel matrices precisely aligned to corresponding tissue classifications. Additional imaging modalities, including thermal and ultrasound sensors, can be integrated to enhance classification accuracy and inform targeted therapeutic placement, providing a comprehensive and personalized approach to wound management for medical and veterinary applications.

A method for producing a curved or tubular collagen scaffold, comprising the steps of: a) providing a substrate; b) cleaning the surface of the substrate; c) functionalizing the surface of the substrate by silanization using a silane or by coating with a metal or carbon; d) depositing one or more layers of a composition comprising collagen onto the substrate; e) inducing self-assembly by adjusting the pH to form one or more layers of nanofibers comprising collagen; f) inducing coiling by fixing the one or more layers of nanofibers to form a curved or tubular nanofiber protein layer comprising collagen;g) Separating the curved or tubular nanofiber protein layer and the substrate, thereby obtaining the curved tubular collagen scaffold, and wherein step f) comprises a further step in which the fixed nanofiber protein layer comprising collagen is allowed to dry.;

The invention provides a culture container of a tissue engineering cartilage scaffold, comprising: a first container having a first opening and a first chamber; the first cover body is mounted on the first container and used for covering the first opening; the second container is used for being placed in the first cavity, the second container is provided with a second opening and a second cavity, the second cavity is used for placing a tissue engineering cartilage scaffold, and the bottom of the second container has a hydrophobic and breathable function, so that a gas medium can enter the second cavity through the bottom of the second container; the second cover body is mounted on the second container and used for covering the second opening, and the second cover body is connected with the first cover body; the jacking and pressing part is mounted on the second cover body, is positioned on one side, deviating from the first cover body, of the second cover body, and is used for limiting the displacement distance of the tissue engineering cartilage scaffold in the second container towards the second opening direction; the technical problem that the collagen scaffold needs to be repeatedly clamped in an existing culture mode is solved, and the collagen scaffold is prevented from floating or adhering to the wall in the culture and transportation process.

A method for preparing a sterilized scaffold for medical use, the method comprising the steps of:i) Loading collagen to a fiber mesh containing fibers of polylactide polymer or copolymer (commonly denoted PLA) to obtain a PLA-collagen scaffold,ii) Drying the PLA-collagen scaffold obtained from step i),iii) Sterilizing the PLA-collagen scaffold obtained from the drying step ii) to obtain the sterilized scaffold.The sterilized scaffold obtained has improved biomechanical properties compared with an unsterilized scaffold.

The present invention discloses a preparation method and application of a carbonate mineralized collagen scaffold material. The preparation method is to first immerse the collagen gel in reagent A, the reaction temperature is 25-37°C, the reaction time is 1-24 hours, and after the reaction is completed, the excess A liquid on the surface of the collagen gel is removed, and the collagen gel is mixed with reagent B for reaction, the reaction temperature is 25-37°C, and the collagen gel is taken out and thoroughly rinsed, and dried to obtain a carbonate mineralized collagen scaffold material. Reagent A is a soluble carbonate and bicarbonate solution with a pH of 9.5-10, and reagent B is a polyelectrolyte-metal cation suspension. The carbonate mineralized collagen scaffold material prepared by this method has a structure in which the inside and outside of the collagen fiber are mineralized simultaneously, and has good biocompatibility and degradability, and slowly and continuously releases functional metal cations by forming insoluble carbonates in collagen to promote bone defect repair.

The application discloses a kind of fibroblast growth factor 2 and mesenchymal cell co-loaded freeze-dried collagen support and its preparation method and application, belong to the technical field of biomedical materials, by adding sulfated polysaccharide to collagen solution, it is formed freeze-dried collagen support containing sulfated polysaccharide by vacuum freeze-drying, it is combined with fibroblast growth factor 2 incubation, obtain the freeze-dried collagen support of loading fibroblast growth factor 2, again mesenchymal cell is inoculated in the freeze-dried collagen support of loading fibroblast growth factor 2, and the freeze-dried collagen support of fibroblast growth factor 2 and mesenchymal cell co-loading is prepared.The support obtained by the application can efficiently combine fibroblast growth factor 2, support the adhesion growth of mesenchymal cell simultaneously, promote mesenchymal cell to migrate to the inside of support, realize the uniform distribution of cell in support, and can promote angiogenesis, provide new and efficient carrier for cell transplantation treatment.

The application relates to the field of tissue engineering and regenerative medicine, and particularly discloses a cell-extracellular matrix complex for hair follicle regeneration and a preparation method thereof. The complex comprises autologous hair follicle stem cells, a human scalp-derived acellular ECM scaffold, time-sequentially added growth factors and an anti-scarring component. In preparation, the autologous hair follicle stem cells are first induced by BMP4 and TGF-beta 1 in cooperation, then inoculated into the human scalp-derived acellular ECM scaffold, and the complex is constructed under dynamic culture conditions, and VEGF is added to promote vascularization. The complex can efficiently form a structure-intact hair papilla-like tissue, has good biocompatibility, low immunogenicity and anti-scarring characteristics, and is significantly superior to a traditional collagen scaffold system. The application provides a new regeneration treatment strategy with clear clinical transformation prospects for scaleable preparation for androgenetic alopecia and other hair follicle deficiency diseases.

The invention discloses a composite material loaded with carbonic anhydrase III. The catalytic activity of the CAR3 is obviously lower than that of other CA subtypes, and analysis finds that the CAR3 has an obvious high expression level and specific distribution characteristics in osteoblasts, so that the composite material prepared by loading the CAR3 with the I-type collagen scaffold can be used for preparing osteoblasts. The composite material loaded with carbonic anhydrase III has a functional structure with good scaffold performance and degradation characteristics, when the composite material is transplanted to a bone defect area, CAR3 can modify collagenous fibers, nucleation sites are formed, crystallization in the collagenous fibers is driven, an ideal repairing environment is achieved, and the composite material is used for repairing bone defects. The composite material loaded with the carbonic anhydrase III, disclosed by the invention, has important significance on repairing bone defects.

The invention relates to a preparation method of a collagen scaffold with a gradient structure and loaded with bioactive components, which comprises the following steps: firstly, fully soaking half of a pure collagen scaffold in a sodium alginate solution, then putting half of the pure collagen scaffold in a calcium chloride solution, and carrying out cross-linking reaction until half of the pure collagen scaffold carries calcium alginate; the collagen scaffold with the calcium alginate is obtained; then completely soaking the collagen scaffold with the calcium alginate in a solution containing a bioactive component, and fully soaking under a low temperature condition, so that the bioactive component is loaded on the other half of the pure collagen scaffold to obtain a primary scaffold; finally, the primary scaffold is placed in a citric acid solution and fully soaked to remove calcium alginate, washing and drying are conducted, and the collagen scaffold with the gradient structure and loaded with the bioactive components is obtained.The collagen scaffold prepared through the method is of a gradual integrated gradient structure without obvious layering in the middle, has good mechanical performance and is good in repairing effect.

The invention relates to the technical field of biology, in particular to a compound polysaccharide-fish skin source collagen scaffold as well as a preparation method and application thereof. According to the preparation method, tilapia skin is taken as a raw material, collagen is extracted and prepared under the condition that the temperature is lower than the denaturation temperature of the collagen, then astragalus polysaccharide and angelica polysaccharide are selected as drugs carried by the collagen scaffold, and the fish-derived collagen scaffold with biological activity is constructed and used for loading bone marrow mesenchymal stem cells (BMSCs). The oxidative stress injury of the BMSCs can be effectively reduced, the proliferation of the BMSCs can be promoted, the diabetic ulcer wound surface can be repaired, and the wound surface healing can be promoted. The stem cell scaffold with biological activity is constructed by utilizing the traditional Chinese medicines astragalus polysaccharide and angelica polysaccharide for the first time, and the advantages of the traditional Chinese medicines are exerted; meanwhile, the collagen adopted by the invention is derived from fish skin instead of mammals, so that religious barriers, mammalian infectious diseases and the like are avoided.

The application provides a preparation method of a collagen in-situ mineralized scaffold simulating a bone matrix, S1: extraction of type I collagen fibers; S2: preparation of a biomimetic mineralization solution: carboxymethyl chitosan (CMC) is placed in deionized water, after stirring and dissolving, K2HPO4 is added, CaCl2.2H2O is added drop by drop, and is fully dissolved and mixed to form a carboxymethyl chitosan / amorphous calcium phosphate biomimetic mineralization solution (CMC / ACP); S3: preparation of a biomimetic collagen scaffold composed of a simulated mineralized bone matrix: the acid-recombined collagen extracted in step S1 is fully mixed with hyaluronic acid (HA) and is placed in a dialysis bag, an external PBS buffer is used to gradually increase the pH value, after self-assembly for a period of time, the CMC / ACP biomimetic mineralization solution prepared in step S2 is added in the dialysis bag and is fully mixed, after mineralization for a period of time, the content of the dialysis bag is centrifuged to remove supernatant, and is repeatedly washed with deionized water, finally, after centrifugation, the precipitate is frozen overnight, and the biomimetic scaffold is finally obtained through freeze-drying. The application provides the preparation method of the collagen in-situ mineralized scaffold simulating the bone matrix.

The invention discloses a preparation method of a bone repair material, and belongs to the technical field of biomedical materials. According to the invention, Solidworks is adopted to construct a scaffold model, a 3D printing technology is utilized to prepare a sodium alginate / chitosan / mineralized collagen scaffold, and a bone repair material with good osteogenesis performance is formed; the mass ratio of the sodium alginate to the chitosan to the mineralized collagen in the material is 1: (0.2-0.6): 0.2. The stent is prepared by using a 3D printing technology, and meanwhile, different chitosan concentrations are selected, so that a series of stents with different properties are obtained; researches find that when the concentration of chitosan in the printing ink is 0.01-0.03 g / mL, the sodium alginate / chitosan / mineralized collagen printing ink forms a stable scaffold in an acidic crosslinking environment; the mechanical property of the stent is in a trend of increasing along with the increase of the concentration of the chitosan; the lower the chitosan concentration of the stent is, the faster the collagen is released in an environment with the pH value of 7.2-7.5, and the larger the release amount is.

The present invention discloses the use of circEIF4B to prepare an agent for promoting healing of tooth extraction wounds in diabetic patients. The use of circEIF4B in the preparation of a preparation or medical device for improving delayed or impaired healing of tooth extraction wounds in diabetic patients. The use of bone marrow mesenchymal stem cells overexpressing circEIF4B in the preparation of a preparation for preserving tooth extraction sites in diabetic patients. BMSCs overexpressing circEIF4B are injected around the extraction socket or implanted into the extraction socket after incubation with a collagen scaffold, for preserving the extraction site of diabetic patients and promoting healing of the extraction socket.