141 results about "Collagen scaffold" patented technology

The subject invention concerns non-degradable three dimensional porous collagen scaffolds and coatings. These scaffolds can be prepared around sensors for implantation into a body. A specific embodiment of the invention concerns implantable glucose sensors. Sensors comprising a collagen scaffold of the invention have improved biocompatibility by minimizing tissue reactions while stimulating angiogenesis. The subject invention also concerns methods for preparing collagen scaffolds of the invention. The subject invention also concerns sensors that have a collagen scaffold of the invention around the exterior of the sensor.

The present invention relates to a heparin-derivatized collagen matrix comprising a fragment of heparin covalently linked to a collagen scaffold, wherein the fragment of heparin has molecular weight of less than about 15 kDa, and at least one heparin-binding growth factor (HBGF) or heparin-binding adeno-associated virus (HB-AAV) or a combination thereof and methods for promoting bone growth, bone repair, cartilage repair, bone development, neo-angiogensis, wound healing, tissue engraftment and muscle tissue regeneration and / or tissue augmentation comprising administering a heparin-derivatized collagen matrix that includes at least one heparin-binding growth factor or heparin-binding adeno-associated virus or a combination thereof.

A collagen scaffold domain, including a collagenous or collagen-like domain, which directs self-trimerization is provided. The collagen scaffold domain can be fused to one or more heterologous domains, such as an antibody domain. Methods for generating and using the scaffold domains and fusion proteins are also provided.

A fiber reinforced basic collagen scaffold with neutralization capacity for delivery of protein based bioactives that have higher solubilities under acidic condition than at neutral pH.

The present invention relates to scaffolds composed of a protein backbone cross-linked by a synthetic polymer. Specifically, the present invention provides PEGylated-thiolated collagen scaffolds and PEGylated albumin scaffolds and methods of generating and using same for treating disorders requiring tissue engineering.

Provided are activated collagen scaffold materials as well as their special fused active restoration factors useful for promoting tissue repair, such as bone damage repair or nerve injury repair. The special fused active restoration factors are fusion proteins comprising a collagen-binding domain (CBD) at N- / C-terminus of cytokines, wherein the collagen-binding domain is a polypeptide consisting of 7-27 amino acid residues with a conservative sequence shown in SEQ IN NO:1 at N-terminus.

The invention relates to a decellularized matrix-source tissue engineering scaffold and a preparation method and an application thereof; the decellularized matrix-source tissue engineering scaffold takes a treated animal membrane material as a biological membrane base material, and the surface of the biological membrane base material is attached with a collagen loose layer. The preparation method of the decellularized matrix source tissue engineering scaffold comprises the process steps of degreasing, decellularizing, antigen removal, cross-linking fixation, collagen extraction, collagen compositing and the like. The flexible and smooth animal membrane material is used as the biological membrane base material, and the defects that a pure collagen scaffold has poor mechanical properties and too fast degradation rate are overcome; with cooperation of the collagen loose layer, a good microenvironment is provided for tissue regenerative repair and cell growth; after being implanted into a body, the decellularized matrix-source tissue engineering scaffold material is gradually degraded along with repairing of defect tissues, also has controllable degradation time, does not exist as a permanent foreign matter, and has no any residual toxicity.

The invention discloses a method for preparing biomimetic artificial bone materials for biodegradable tissue engineering. The method comprises the following steps: adding a hydroxyapatite suspension to a human-like collagen solution; adding Genipin for crosslinking; performing vacuum freeze-drying to obtain human-like collagen / hydroxyapatite powder; adding the human-like collagen / hydroxyapatite powder to a chitosan solution; using an NaOH solution to adjust the pH of the solution to 7.0; repeatedly adding the human-like collagen solution and the chitosan solution; adding a Genipin solution for crosslinking; freeze-drying a mixed solution; inoculating the obtained material with BMSCs of which the cell density is 5*104 cells / cm2; performing culture for 1 to 10 days; using an induction medium to culture BMSCs cells so as to induce the BMSCs cells to differentiate towards an osteogenesis direction; and forming bone repair composite materials with bone inducing activity. The artificial bone materials prepared by the method are excellent in bone inducing activity and immune compatibility, thereby thoroughly putting an end to inevitable viral hidden trouble of animal collagen scaffold materials and greatly improving use safety.

A method for producing collagen-based scaffolds with improved characteristics, which broadens the usage of collagen in tissue engineering and the products so produced are described. The method comprises reconstitution of three-dimensional collagen matrices from collagen monomer solution and crosslinking the matrix with a light source in the presence of a photosensitizing reagent. The crosslinked products can be in any shape and form and used in the dry or wet state, for applications including but not limited to tissue engineering and controlled drug delivery.

A putty form biomaterial composition made of a collagen scaffold and ceramic granule having improved flowable yet cohesive characteristics through the addition of, either individually or in some combination, polyhydroxy compounds, a liquid polyhydroxy compound ester, a liquid solution of solid polyhydroxy compound, a liquid solution of solid polyhydroxy compound ester to allow for use in surgical bone repair is presented. Specific polyhydroxy compounds, including polyethylene glycol polymers (PEG), PPO / PEO block co-polymers (i.e., a poloxamer NF grade), and the polysaccharides alginate and chitosan may be utilized.

The invention relates to a method for producing a multi-layer collagen scaffold. The method generally comprises the steps of: preparing a first suspension of collagen and freezing or lyophilising the suspension to provide a first layer; optionally preparing a further suspension of collagen and adding the further suspension onto the layer formed in the previous step to form a further layer, and freezing or lyophilising the layers, wherein when the layer formed in the previous step is formed by lyophilisation the lyophilised layer is re-hydrated prior to addition of the next layer; optionally, repeating the aforementioned step to form one or more further layers; and preparing a final suspension of collagen and pouring the final suspension onto the uppermost layer to form a final layer, and freeze-drying the layers to form the multilayer collagen composite scaffold.

The invention relates to a preparation method and application of collagen scaffold composite bone marrow-derived mesenchymal stem cells (BMSCs). The preparation method comprises the steps of preparing single cell suspension after trypsinizing the BMSCs, uniformly dropwise adding the single cell suspension to a collagen scaffold, putting the collagen scaffold into an incubator to be cultured, and adding an L-DMEM complete medium to continue culture, thus obtaining the collagen scaffold composite BMSCs. The preparation method has the advantages that the following defects are overcome: endometria are seriously injured as intrauterine adhesion is mechanically separated by adopting hysteroscopic surgery, intrauterine devices or anti-adhesion materials are put after the surgery and estrogens are given after the surgery to promote intima growth; the problem of intima scars can not be solved; functional intima repair can not be achieved; adhesion is very easy to happen again. As active ingredients for treating serious endometrium injury, the BMSCs are convenient to obtain, secrete growth factors to improve the local microenvironment and immunoregulation, have good biocompatibility, degradability and safety, promote scarred endometrium repair and increase the intima thickness and local blood vessel density.

Bioimplants and methods of making the bioimplants are provided. The bioimplants comprise biological tissues having conjugated thereto adjunct molecules. The biological tissues are sterilized with a chemical sterilizing agent, such as a water soluble carbodiimide. The processes of making the bioimplants include a process in which an adjunct molecule is conjugated to a biological tissue during the sterilization process.

The invention discloses a method for preparing a collagen material for repairing peripheral nerve injury. The method comprises the following step: co-incubating a ciliary neurotrophic factor with a collagen binding domain (CBD-CNTF), a basic fibroblast growth factor with a collagen binding domain (CBD-bFGF) and an orderly collagen material to obtain a collagen material for repairing the peripheral nerve injury. According to the preparation method, two factors CBD-CNTF and CBD-bFGF are simultaneously crosslinked to the orderly collagen scaffold material, and the action of the double-factor peripheral nerve injury repairing collagen material in long-cross-section nerve injury repairing can be evaluated by establishing an animal peripheral nerve-facial nerve long cross-section injury model. Results prove that compared with single factor CBD-CNTF or CBD-bFGF functional collagen scaffold material, the double-factor peripheral nerve injury repairing collagen material can be used for better promoting regeneration of facial nerves and restoration of functions, and has a more important realistic guiding magnificence to application of clinical peripheral nerve injury repairing in the future.

The present invention relates to a method for inducing the bionic calcification in collagen fibers through a polymer polyelectrolyte, and applications thereof. The method comprises: treating different types of collagen scaffolds in a calcium phosphate mineralized solution containing a cationic polyelectrolyte or anionic polyelectrolyte to obtain the bionic calcification collagen scaffold material having the inside-fiber mineralization characteristic, wherein the collagen scaffold is recombinant collagens type I having different sources, completely demineralized bone collagen, completely demineralized dentin collagen, or rat tail collagen, and the mineralization liquid is a calcium phosphate solution added with a cationic polyelectrolyte or anionic polyelectrolyte. According to the present invention, through the stabilizing effect of the polyelectrolyte on the calcium phosphate solution, the novel bionic calcified collagen scaffold material with characteristics of similar natural bone tissue structure, good mechanical property, good biocompatibility and suitable biodegradability is constructed.

The invention provides a preparation method of a bionic mineralized collagen scaffold. The preparation method comprises the following steps: 1), preparing a bionic mineralized liquid: adjusting the pHvalue of carboxymethyl chitosan-amorphous calcium phosphate (or amorphous strontium phosphate or amorphous strontium carbonate) nanocomposite liquid to be lower than an isoelectric point of CMC; 2),preparing the bionic mineralized collagen scaffold: mixing the bionic mineralized liquid and acid-dissolved type-I collagen, then putting into a dialysis bag, sealing, then putting into a PBS buffer liquid, self-assembling and mineralizing for 2-5 days, then adding an alkaline CMC / ACP (ASP or ASC) liquid into the dialysis bag, and changing the PBS buffer liquid; 1-3 days later, putting the dialysis bag into deionized water for dialyzing for 10-72h, changing the deionized water for several times, centrifuging the liquid in the bag to obtain collagen gel, and performing freeze drying to obtain the bionic mineralized collagen scaffold. By the preparation method, collagen self-assembly and mineralization are cooperatively performed, intra-collagen fiber mineralization is achieved at relativelyhigh efficiency, and osteogenic ability-promoting strontium is integrated into the collagen scaffold, so that the preparation method has a wide clinical application prospect.

A fabric for use in the human body composed of non-absorbable micro diameter threads contained in absorbable materials, such that over time the absorbable material dissolves or is absorbed by the body and the non-absorbable micro diameter threads cause the body to create a collagen scaffold transferring load from the absorbable material to the collagen scaffold. The fabric can be coated or impregnated with materials to reduce infection, provide tissue growth, reduce scar tissue or other medical purpose. Threads of non-absorbable material can be coated with absorbable material to create a fabric.

A method and means of providing stromal repair and improved refractive correction by creating corneal stromal collagen tissue with fibril diameter and spacing that duplicates the optical transmission and diffusion characteristics of natural corneal collagen. The repair method includes implanting the collagen scaffold during laser corneal ablation or other interlamellar surgery to improve visual acuity or to preclude the possibility of ectasia

A bioscaffold and method of manufacture is described. The bioscaffold comprised greater than 80% type I collagen fibers or bundles having a knitted structure providing tensile load strength. The method of manufacture comprises the steps of: (a) isolating collagen fibers or bundles; (b) incubating said fibers or bundles in a mixture of NaOH, alcohol, acetone, HC; and ascorbic acid; and (c) mechanical manipulation of said fibers or bundles to produce a knitted structure.

The invention discloses a method for preparing a nano hydroxyapatite / collagen scaffold with directionally arranged particles. The method takes polyvinylpyrrolidone as a template, a dispersant and a modifying agent simultaneously; the polyvinylpyrrolidone is fully mixed in an alkaline agent control system to form a sol system with the molar ratio of 1.6 to 1.7:1 between calcium and phosphorus and the content of 0.01 to 50 percent for the polyvinylpyrrolidone according to mass percent; the sol system is aged and washed, thus obtaining nano hydroxyapatite sol; then mixing and high-speed dispersion are conducted according to the proportion of 0.1 to 9:1 between the nano hydroxyapatite sol and a collagen solute according to mass ratio; and the mixture of the nano hydroxyapatite sol and the collagen solute is injected into a mould and refrigerated and dried, thus obtaining the nano hydroxyapatite / collagen scaffold. The hydroxyapatite particles in the scaffold are well dispersed in a collagenbasal body, form effective and stable linkage with collagens and are directionally arranged, thereby realizing a simulated structure of a natural bone from the view of the structure and the function.The method has simple technique, fast and convenient operation and easy promotion and application.

The invention provides a stem cell composite collagen scaffold kit for repairing endometrial damage and a preparation method thereof, which consists of human umbilical cord mesenchymal stem cells and collagen scaffolds. The preparation method comprises the steps of recovery of umbilical cord mesenchymal stem cells, passage of umbilical cord mesenchymal stem cells, and compound culture of umbilical cord mesenchymal stem cells and collagen scaffolds. The kit of the present invention uses collagen scaffolds for umbilical cord mesenchymal stem cells to repair the endometrium, and has the following advantages compared with traditional endometrial repair methods: the presence of collagen scaffolds can provide attachment sites for umbilical cord mesenchymal stem cells to position them In the damaged part of the intima, it is conducive to local tissue repair, improves the effectiveness of repair and reduces the risk of cell translocation; at the same time, the collagen scaffold can promote cell differentiation.

The invention discloses a nerve scaffold for nerve injury repairing and a preparing method and application thereof. The nerve scaffold comprises a collagen scaffold body, neural stem cells cultured on the collagen scaffold body and growth factors fixed to the collagen scaffold body through heparin. The growth factors have heparin binding domains. The preparing method includes the steps that a collagen solution is subjected to freeze drying before crosslinking, a collagen sponge obtained after freeze drying is soaked into a crosslinking solution to be crosslinked, the growth factors are fixed to the collagen scaffold body through the heparin, and the activity of the neural stem cells is maintained or propagation of the neural stem cells is promoted. According to the preparing method, materials are convenient to obtain, the steps are simple, complex steps including acid-base neutralization, dialysis and the like are avoided, the opportunity that collagen materials are in contact with harmful reagents is reduced, the safety of the obtained nerve scaffold is improved, the nerve scaffold can be molded to be in different shapes as required and can be used for filling nervous tissue losses and bridged nerve broken ends and guiding growth of nerves, nerve injury repairing and function recovery are promoted accordingly, and good stability is achieved.

The invention discloses a BMP loaded silk fibroin / collagen scaffold material. The material is prepared with collagen, BMP, silk fibroin and PLGA as raw materials; a preparation method comprises the steps of preparation of a loose-layer silk fibroin / collagen membrane and a dense-layer silk fibroin / collagen membrane, preparation of BMP loaded PLGA microspheres, composite of the silk fibroin / collagen membranes and the microspheres and the like. A scaffold is a tissue engineering scaffold having no toxic or side effect on bodies and having good biocompatibility and tissue repair capacity. Based on combination of respective advantages of good biological compatibility, biological degradation and the like of two different natural biological macromolecules of silk fibroin and collagen, the BMP is introduced to the scaffold, a directional controlled-release slow-release technology is adopted, the BMP is allowed to be released persistently, the problems of relatively short in-vivo half-life period of the BMP, growth factor directional introduction local defect repairing and the like are solved effectively, and full playing of functions are prolonged.

The invention provides a fibrous internal and external mineralized collagen scaffold and a preparation method thereof. The fibrous internal and external mineralized collagen scaffold material not only is approximate to natural osseous tissues on chemical composition but also simulates the generation process of natural osseous tissues on formation process as far as possible, so that a characteristic and periodic (D=67 nm) transverse position structure in collagen fibers. The novel biomimetic mineralized scaffold material is remarkably superior to a scaffold material prepared according to the traditional mineralizing method in in-vivo experiments (animal experiments), in-vitro experiments (cell proliferation and osteogenic differentiation) and mechanical property experiments, thereby providing a new probability for repair of clinical osseous tissue defects in future and having a wider application prospect.

The invention discloses a herniorrhaphy sheet and a preparation method thereof. The preparation method comprises the following steps of peeling sorting, degreasing and decellularization, cutting and irradiation sterilization. The preparation method of the herniorrhaphy sheet provided by the invention has the advantages that the use of strong acid, strong alkali and enzymes is avoided; a natural three-dimensional collagen scaffold fiber structure of the pig skin substrate can be remained, so that the herniorrhaphy sheet has good mechanical performance so as to achieve the effects of reinforcing or restoring defect tissues; the prepared herniorrhaphy sheet can completely remain the natural structure of the pig skin substrate; good tissue compatibility is achieved; no virus spreading hidden danger exists.

The invention discloses a breast patch and a preparation method thereof. The preparation method comprises the following steps of: paring and sorting; degreasing and decellularizing; cutting; irradiating and sterilizing. By adopting the preparation method of the breast patch provided by the invention, application of strong acid, strong alkali and enzyme can be avoided, the natural three-dimensional collagen scaffold fiber structure of porcine acellular dermal matrix can be maintained, and the breast patch has excellent mechanical performance to achieve the effect of strengthening or repairing defective tissues. The prepared breast patch has tidy edge and smooth curve, can meet basic requirement for medical appliance products, and guarantees biological safety.

The invention belongs to the field of cytobiology and specifically relates to an in vitro three-dimensional culture model for research on vasculogenic mimicry of glioma stem cells and an application thereof. Preparation of the in vitro three-dimensional culture model comprises the following steps: immersing a three-dimensional collagen scaffold in a DMEM culture medium for 6-24 h, taking out the three-dimensional collagen scaffold, removing superfluous DMEM adhered to the three-dimensional collagen scaffold to obtain a pretreated three-dimensional collagen scaffold, placing the pretreated three-dimensional collagen scaffold on a cell culture plate, culturing sphere-formed glioma stem cells in the pretreated three-dimensional collagen scaffold, standing at 37 DEG C for 2-6 h, adding an endothelial medium, and culturing at 37 DEG C for 2-4 days, so as to obtain the in vitro three-dimensional culture model for research on vasculogenic mimicry of glioma stem cells. Each operational process of the in vitro three-dimensional culture model is carried out at room temperature; various kinds of in situ staining, such as immunohistochemical staining and immunofluorescent staining, can be carried out; electron microscopic examination can be carried out; and RT-PCR and Western Blot detection can be carried out directly after digestion.