29 results about "Collagen scaffold" patented technology

A biologic matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized, suspension-dried mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more of its outer surfaces. The pores are created by forcing a gas through the vascular pathways of the mammalian organ or tissue while it is in a suspended position. This enables the gas to inflate the vascular pathways in all directions without restriction. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 10 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 15 times larger or more than the non-porous scaffold of equivalent outer dimensions.

The invention provides a double-layer collagen scaffold repair membrane as well as a preparation method and application thereof, and the preparation method comprises the following steps: extracting collagen from animal fur, and emulsifying to obtain a collagen emulsion; adding a cross-linking agent into the collagen emulsion, and emulsifying to obtain a cross-linked protein emulsion; pouring the cross-linked protein emulsion into a mold, and freeze-drying to obtain a cross-linked protein layer; and pouring the collagen emulsion on the cross-linked protein layer, and freeze-drying to obtain the double-layer collagen scaffold repair membrane. The repairing membrane is excellent in structure, the cross-linked protein layer provides mechanical strength and supporting performance, the collagen layer can be rapidly dissolved and attached to the contact surface, and oxygen exchange and metabolite discharge of the wound surface are facilitated; the collagen is high in purity, low in antigenicity and high in safety, any toxic and harmful chemical reagents and preservatives are not added, allergy or inflammatory reaction is avoided, and the collagen has good application prospects in the fields of medical treatment, medical beauty, beauty, skin care and the like.

The invention discloses a high-strength self-lubricating three-layer nested medicine-carrying hollow cervical cerclage band with an auxiliary miscarriage prevention function and a preparation method of the cerclage band, and belongs to the technical field of medical instruments. Degradable polyester monofilaments are woven into a hollow round rope, and the hollow round rope and a medical curved needle are integrated; then carrying out polydopamine-polyethylene glycol double-layer modification on a copper-based metal organic framework (Cu-MOF), and loading active molecules; then mixing the drug-loaded Cu-MOF with collagen natural polymer sol, and uniformly filling the gel into the inner cavity of the round rope and forming a three-dimensional bracket through synchronous drawing type micro-perfusion and light-enzyme synergistic cross-linking; then constructing a transient hydrated lubricating layer on the surface; and finally carrying out ethylene oxide final sterilization to obtain a finished product. The cervical cerclage band device provided by the invention simultaneously provides degradable mechanical support, collagen scaffold induction and long-acting slow release regulation, has a low-friction implantation characteristic, and can be well used for preventing or treating middle and late abortion and premature delivery caused by cervical insufficiency.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.

The application aims to realize self-adhesive fixation of cartilage repair scaffolds, and provides a collagen scaffold preparation method with controllable removal of terminal peptides, comprising the following steps: S1, cleaning and shearing collagen raw materials to obtain granular collagen materials; S2, dehydrating, centrifuging and dispersing the granular collagen materials to obtain dispersed collagen materials; S3, performing enzymatic treatment on the dispersed collagen materials to obtain a collagen solution; and S4, preparing a collagen scaffold by using the collagen solution, wherein the removal rate of terminal peptides in the collagen solution is controlled at 60%-80% in step S3, which comprises: adding water, glacial acetic acid and pepsin into the dispersed collagen materials according to the mass ratio of dispersed collagen materials:water:glacial acetic acid:pepsin=1:(5-25):(0.3-1.0):(0.05-1.5); keeping at a temperature of 4-8 DEG C for 48 h-120 h, and stirring several times during the keeping period; and performing low-temperature gradient dialysis under a temperature condition of 4-8 DEG C to remove residual enzyme solution, thereby obtaining the collagen solution. The application further provides the collagen scaffold prepared by the method and application thereof.

A sterilized PLA-collagen scaffold comprising animal collagen and a method for preparing a sterilized scaffold for medical use, the method comprising the steps of:i) Loading animal collagen to a fiber mesh containing fibers of polylactide polymer or copolymer (commonly denoted PLA) to obtain a PLA-collagen scaffold,ii) Drying the PLA-collagen scaffold obtained from step i),iii) Sterilizing the PLA-collagen scaffold obtained from the drying step ii) to obtain the sterilized scaffold.The sterilized scaffold obtained has improved biomechanical and physical properties compared with an unsterilized scaffold.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.

The application provides a preparation method of a mineralized collagen scaffold medical material prepared by a polymer-induced liquid precursor, and phosphine polyethyleneimine and polyacrylic acid are used in a high-concentration calcium phosphate solution to prepare a polymer-induced liquid precursor; collagen fiber scaffolds are immersed in the polymer-induced liquid precursor to be mineralized to obtain the mineralized collagen scaffold medical material; the polymer-induced liquid precursor comprises the following components in percentage by mass: 1-3% of phosphine polyethyleneimine, 4-6% of polyacrylic acid, 0.5-1% of calcium chloride, 2.5-3% of disodium hydrogen phosphate and the balance of water. The PILP in the application can penetrate into the inside of the collagen scaffold to be mineralized, the mineralization effect of the collagen scaffold is enhanced, the biocompatibility and osteogenic activity of the collagen scaffold are improved, and the application prospect of the PILP in the field of bone repair materials is wide.

A multispectral photonic imaging system and associated methods are disclosed for classifying wound tissue and generating customized therapeutic wound dressings. The system employs wavelength-specific optical sources and integrated image sensors to capture multispectral reflectance data from a wound, enabling computational classification of distinct tissue types such as granulation, slough, and necrosis. A three-dimensional (3D) digital model of the wound is constructed using spatial data from depth sensors. The classified 3D wound model facilitates automated design of patient-specific dressings, featuring heterogeneous regions tailored with therapeutic compounds like antimicrobial agents, analgesics, collagen scaffolds, and hydrogel matrices precisely aligned to corresponding tissue classifications. Additional imaging modalities, including thermal and ultrasound sensors, can be integrated to enhance classification accuracy and inform targeted therapeutic placement, providing a comprehensive and personalized approach to wound management for medical and veterinary applications.

A method for producing a curved or tubular collagen scaffold, comprising the steps of: a) providing a substrate; b) cleaning the surface of the substrate; c) functionalizing the surface of the substrate by silanization using a silane or by coating with a metal or carbon; d) depositing one or more layers of a composition comprising collagen onto the substrate; e) inducing self-assembly by adjusting the pH to form one or more layers of nanofibers comprising collagen; f) inducing coiling by fixing the one or more layers of nanofibers to form a curved or tubular nanofiber protein layer comprising collagen;g) Separating the curved or tubular nanofiber protein layer and the substrate, thereby obtaining the curved tubular collagen scaffold, and wherein step f) comprises a further step in which the fixed nanofiber protein layer comprising collagen is allowed to dry.;

The invention provides a culture container of a tissue engineering cartilage scaffold, comprising: a first container having a first opening and a first chamber; the first cover body is mounted on the first container and used for covering the first opening; the second container is used for being placed in the first cavity, the second container is provided with a second opening and a second cavity, the second cavity is used for placing a tissue engineering cartilage scaffold, and the bottom of the second container has a hydrophobic and breathable function, so that a gas medium can enter the second cavity through the bottom of the second container; the second cover body is mounted on the second container and used for covering the second opening, and the second cover body is connected with the first cover body; the jacking and pressing part is mounted on the second cover body, is positioned on one side, deviating from the first cover body, of the second cover body, and is used for limiting the displacement distance of the tissue engineering cartilage scaffold in the second container towards the second opening direction; the technical problem that the collagen scaffold needs to be repeatedly clamped in an existing culture mode is solved, and the collagen scaffold is prevented from floating or adhering to the wall in the culture and transportation process.

The application discloses a kind of fibroblast growth factor 2 and mesenchymal cell co-loaded freeze-dried collagen support and its preparation method and application, belong to the technical field of biomedical materials, by adding sulfated polysaccharide to collagen solution, it is formed freeze-dried collagen support containing sulfated polysaccharide by vacuum freeze-drying, it is combined with fibroblast growth factor 2 incubation, obtain the freeze-dried collagen support of loading fibroblast growth factor 2, again mesenchymal cell is inoculated in the freeze-dried collagen support of loading fibroblast growth factor 2, and the freeze-dried collagen support of fibroblast growth factor 2 and mesenchymal cell co-loading is prepared.The support obtained by the application can efficiently combine fibroblast growth factor 2, support the adhesion growth of mesenchymal cell simultaneously, promote mesenchymal cell to migrate to the inside of support, realize the uniform distribution of cell in support, and can promote angiogenesis, provide new and efficient carrier for cell transplantation treatment.

The application relates to the field of tissue engineering and regenerative medicine, and particularly discloses a cell-extracellular matrix complex for hair follicle regeneration and a preparation method thereof. The complex comprises autologous hair follicle stem cells, a human scalp-derived acellular ECM scaffold, time-sequentially added growth factors and an anti-scarring component. In preparation, the autologous hair follicle stem cells are first induced by BMP4 and TGF-beta 1 in cooperation, then inoculated into the human scalp-derived acellular ECM scaffold, and the complex is constructed under dynamic culture conditions, and VEGF is added to promote vascularization. The complex can efficiently form a structure-intact hair papilla-like tissue, has good biocompatibility, low immunogenicity and anti-scarring characteristics, and is significantly superior to a traditional collagen scaffold system. The application provides a new regeneration treatment strategy with clear clinical transformation prospects for scaleable preparation for androgenetic alopecia and other hair follicle deficiency diseases.

The invention discloses a composite material loaded with carbonic anhydrase III. The catalytic activity of the CAR3 is obviously lower than that of other CA subtypes, and analysis finds that the CAR3 has an obvious high expression level and specific distribution characteristics in osteoblasts, so that the composite material prepared by loading the CAR3 with the I-type collagen scaffold can be used for preparing osteoblasts. The composite material loaded with carbonic anhydrase III has a functional structure with good scaffold performance and degradation characteristics, when the composite material is transplanted to a bone defect area, CAR3 can modify collagenous fibers, nucleation sites are formed, crystallization in the collagenous fibers is driven, an ideal repairing environment is achieved, and the composite material is used for repairing bone defects. The composite material loaded with the carbonic anhydrase III, disclosed by the invention, has important significance on repairing bone defects.

The invention relates to a preparation method of a collagen scaffold with a gradient structure and loaded with bioactive components, which comprises the following steps: firstly, fully soaking half of a pure collagen scaffold in a sodium alginate solution, then putting half of the pure collagen scaffold in a calcium chloride solution, and carrying out cross-linking reaction until half of the pure collagen scaffold carries calcium alginate; the collagen scaffold with the calcium alginate is obtained; then completely soaking the collagen scaffold with the calcium alginate in a solution containing a bioactive component, and fully soaking under a low temperature condition, so that the bioactive component is loaded on the other half of the pure collagen scaffold to obtain a primary scaffold; finally, the primary scaffold is placed in a citric acid solution and fully soaked to remove calcium alginate, washing and drying are conducted, and the collagen scaffold with the gradient structure and loaded with the bioactive components is obtained.The collagen scaffold prepared through the method is of a gradual integrated gradient structure without obvious layering in the middle, has good mechanical performance and is good in repairing effect.

The invention relates to the technical field of biology, in particular to a compound polysaccharide-fish skin source collagen scaffold as well as a preparation method and application thereof. According to the preparation method, tilapia skin is taken as a raw material, collagen is extracted and prepared under the condition that the temperature is lower than the denaturation temperature of the collagen, then astragalus polysaccharide and angelica polysaccharide are selected as drugs carried by the collagen scaffold, and the fish-derived collagen scaffold with biological activity is constructed and used for loading bone marrow mesenchymal stem cells (BMSCs). The oxidative stress injury of the BMSCs can be effectively reduced, the proliferation of the BMSCs can be promoted, the diabetic ulcer wound surface can be repaired, and the wound surface healing can be promoted. The stem cell scaffold with biological activity is constructed by utilizing the traditional Chinese medicines astragalus polysaccharide and angelica polysaccharide for the first time, and the advantages of the traditional Chinese medicines are exerted; meanwhile, the collagen adopted by the invention is derived from fish skin instead of mammals, so that religious barriers, mammalian infectious diseases and the like are avoided.

The application provides a preparation method of a collagen in-situ mineralized scaffold simulating a bone matrix, S1: extraction of type I collagen fibers; S2: preparation of a biomimetic mineralization solution: carboxymethyl chitosan (CMC) is placed in deionized water, after stirring and dissolving, K2HPO4 is added, CaCl2.2H2O is added drop by drop, and is fully dissolved and mixed to form a carboxymethyl chitosan / amorphous calcium phosphate biomimetic mineralization solution (CMC / ACP); S3: preparation of a biomimetic collagen scaffold composed of a simulated mineralized bone matrix: the acid-recombined collagen extracted in step S1 is fully mixed with hyaluronic acid (HA) and is placed in a dialysis bag, an external PBS buffer is used to gradually increase the pH value, after self-assembly for a period of time, the CMC / ACP biomimetic mineralization solution prepared in step S2 is added in the dialysis bag and is fully mixed, after mineralization for a period of time, the content of the dialysis bag is centrifuged to remove supernatant, and is repeatedly washed with deionized water, finally, after centrifugation, the precipitate is frozen overnight, and the biomimetic scaffold is finally obtained through freeze-drying. The application provides the preparation method of the collagen in-situ mineralized scaffold simulating the bone matrix.

The invention discloses a preparation method of a bone repair material, and belongs to the technical field of biomedical materials. According to the invention, Solidworks is adopted to construct a scaffold model, a 3D printing technology is utilized to prepare a sodium alginate / chitosan / mineralized collagen scaffold, and a bone repair material with good osteogenesis performance is formed; the mass ratio of the sodium alginate to the chitosan to the mineralized collagen in the material is 1: (0.2-0.6): 0.2. The stent is prepared by using a 3D printing technology, and meanwhile, different chitosan concentrations are selected, so that a series of stents with different properties are obtained; researches find that when the concentration of chitosan in the printing ink is 0.01-0.03 g / mL, the sodium alginate / chitosan / mineralized collagen printing ink forms a stable scaffold in an acidic crosslinking environment; the mechanical property of the stent is in a trend of increasing along with the increase of the concentration of the chitosan; the lower the chitosan concentration of the stent is, the faster the collagen is released in an environment with the pH value of 7.2-7.5, and the larger the release amount is.

The present invention discloses a preparation method and application of a bionic multi-stage spherical mineralized collagen scaffold. The collagen mineralization area of ​​the prepared mineralized collagen scaffold presents a specific distribution, and a bionic multi-stage spherical structure appears, which can highly simulate the mineralization pattern of the mineralization front of the organism. The mineralized collagen scaffold is formed by inducing the mineralization of type I collagen within collagen fibers by a specific mineralization system prepared in vitro, and presents a spherical structural unit after multi-stage assembly. The above-mentioned specific mineralization system is a calcium-phosphate precursor system stabilized by egg yolk phosphoprotein. The bionic multi-stage spherical mineralized collagen scaffold material of the present invention has excellent bone induction properties and unique mechanical properties, and is non-cytotoxic, and can be used as a bone tissue engineering scaffold material to promote bone defect repair.

The present invention discloses a method for preparing a collagen scaffold and its application, relating to the field of biomedical materials technology. This method utilizes bovine fascia as raw material and utilizes the coordinated parameters of various steps to produce a porous, transparent collagen scaffold material suitable for tumor cell adhesion and migration. The collagen material prepared by this method significantly improves gel strength and can more effectively reveal the pathological characteristics of tumors in vivo. The method does not require the addition of traditional chemical crosslinkers, significantly reducing the risks of solvent residue, cytotoxicity, active denaturation, and calcification.

Shape-stabilized collagen scaffolds and methods of obtaining such scaffolds are disclosed. Stroma can be harvested, for example, from human or porcine corneal stroma and shaped during excision or in a separate step after excision. Following shaping (and preferably decellularization), the excised stroma portion is subject to pressure, force or vacuum to reduce fluid content and then irradiated or otherwise treated to induce crosslinking of collagen chains or fibrils. In one embodiment, the scaffold can be compacted by removing some or all of the water from the scaffold, and rehydrating the scaffold in a controlled manner (e.g., in a mold or other confining space) such that the scaffold takes a desired compacted shape; and then crosslinking at least a portion of the scaffold to mechanically strengthen it and inhibit subsequent swelling. Various sources of energy can be employed to induce crosslinking of collagen including, for example, ultraviolet (UV) radiation. The scaffolds can also be selectively densified or patterned. The invention is particularly useful in forming stable lenticules of enhanced stiffness and sufficient optical clarity for intracorneal implantation in additive ocular surgery.

The invention discloses a mesenchymal stem cell exosome-loaded PRP collagen artificial skin scaffold and a method, and relates to the technical field of tissue engineering in biomedical engineering. Based on the synergistic effect of the three-dimensional reticular porous structure of the composite matrix layer and the function regulation and control layer, the mechanical property and the biological function are doubly improved, the elastic modulus of the composite matrix layer reaches 463 + / -0.252 kPa, the ultimate tensile strength is 220.6 + / -3.725 kPa, and is remarkably higher than that of a traditional collagen scaffold (smaller than 400 kPa), stress distribution is more uniform, and the composite matrix layer has a good application prospect. Finite element analysis shows that the shear stress peak value is reduced to 0.5 MPa or below, so that the risk of wound edge cracking is reduced; through the synergistic effect of the slow release mechanism of the exosome in the function regulation and control layer and the sodium hyaluronate-chitosan composite system, the effective regulation and control of the wound surface microenvironment are realized.

The invention relates to the technical field of biomedical engineering and tissue regeneration, and particularly discloses a functionalized collagen scaffold based on 3D printing and application of the functionalized collagen scaffold in achilles tendon repair and heterotopic ossification prevention. And integrating the CD26 + tendon stem cell exosome which is pretreated by the BMP inhibitor and modified by the collagen binding peptide to form the 3D printing scaffold. According to the invention, the composite scaffold is prepared by integrating a bifunctional strategy of BMP signal inhibition and collagen targeted slow release and combining a 3D printing technology, so that the problems of insufficient regeneration effect and high incidence of heterotopic ossification (HO) in the existing tendon repair technology are obviously solved.

The invention discloses a bone repair material obtained by taking margarine scale collagen as a biomineralization template and a preparation method of the bone repair material. The bone repair material is obtained by biomineralization treatment of a margarine scale collagen scaffold obtained by removing minerals from margarine scales with a Brini spine structure. The bone repair material obtained by taking the scale of the margarya scales as the collagen scaffold has adjustable mechanical properties, the bone repair materials with different mineralization degrees and the stacking thickness of the bone repair materials can be selected according to the use scene of a bone defect area so as to meet different mechanical property requirements, and the bone repair material has excellent biocompatibility; as a bone repair material, the material has a good application prospect.

The application provides a preparation method of a mineralized collagen scaffold material with a biomimetic structure, and belongs to the field of biomedical engineering.The preparation method comprises the following steps: preparing a polyacrylic acid-stabilized amorphous calcium phosphate solution, wherein the molar ratio of Ca element to P element in the amorphous calcium phosphate solution is 1.67:1; preparing a collagen solution containing norepinephrine; performing isoelectric focusing treatment on the collagen solution under a constant voltage to form a dense film, then stretching the dense film to produce a deformation of 120% to 200%, and obtaining a collagen film with a biomimetic structure; soaking the collagen film in the amorphous calcium phosphate solution, and then performing freeze-drying to obtain a norepinephrine-mineralized collagen scaffold material.The mineralized collagen scaffold material prepared by the method has excellent biocompatibility, osteogenic induction and sufficient mechanical strength, can effectively promote bone tissue defect repair, and has a good application prospect in the field of bone tissue engineering.The application further provides a mineralized collagen scaffold material with a biomimetic structure and an application thereof.

This invention discloses a method for preparing a collagen scaffold based on dynamic temperature control, and the collagen scaffold itself, relating to the field of biomedical materials technology. The method includes: preparing a collagen slurry; injecting the collagen slurry into a mold with insulated sidewalls and a heat-conducting bottom; implementing dynamic temperature-controlled directional freezing through pre-cooling and isothermal nucleation, cold source pre-cooling, linear continuous cooling freezing, and low-temperature shaping; followed by freeze-drying and post-processing to obtain the collagen scaffold. This invention avoids random, explosive nucleation by forming uniform, fine ice crystal nuclei near the freezing point through pre-cooling and isothermal nucleation; cold source pre-cooling and isothermal stabilization avoids unsteady temperature fields caused by cold source temperature fluctuations; and linear continuous cooling precisely controls the unidirectional growth rate of ice crystals, synergistically achieving a high degree of orientation of collagen fibers and a uniform distribution of axial pore size in the scaffold. The resulting collagen scaffold possesses high orientation, small pore size fluctuations, and excellent mechanical properties and biocompatibility, and can be widely used in the repair of oriented tissues such as tendons, ligaments, and nerves.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.