387 results about "Genipin" patented technology

A method for treating vulnerable plaques of a patient, comprising: providing a biodegradable stent comprising a first supporting zone made of a first biodegradable material, wherein the supporting zone comprises at least a portion of continuous circumference of the stent; and a second therapeutic zone made of a second biodegradable material, wherein the therapeutic zone comprises at least one bioactive agent; delivering the biodegradable stent to the vulnerable plaques; orienting the therapeutic zone at about the luminal surface of the vulnerable plaque; and releasing the at least one bioactive agent for treating the vulnerable plaques.

A method for promoting autogenous ingrowth of damaged or diseased tissue selected from a group consisting of ligaments, tendons, muscle and cartilage, the method comprising a step of surgically repairing the damaged or diseased tissue by attachment of a tissue graft, wherein the tissue graft is formed from a segment of connective tissue protein after an acellularization process, the segment being crosslinked with genipin, its analog or derivatives. The genipin-fixed acellular tissue provides a microenvironment for tissue regeneration adapted for use as a biological implant device due to its low cytotoxicity.

The present invention relates to novel genipin derivatives which have an excellent liver protection activity with little cytotoxicity, and these compounds are so stable in vivo that they do not induce any side effects.

A method of preparing colored products from edible materials comprises processing Genipa americana fruit juice, which contains genipin, genipin derivatives, or pre-genipin compounds, with other edible juices or extracts which contain nitrogenous compounds such as amino acids, polypeptides, or proteins. The generated natural colored products have excellent stability and can be used in a broad range of applications including beverages, foodstuffs, drugs, dietary supplements, cosmetics, personal care stuffs, and animal feeds.

A method for treating a target tissue of a patient comprising, in combination, mixing a drug with a solidifiable biological material, chemically treating the drug with the biological material with a crosslinking agent, loading the solidifiable drug-containing biological material onto a medical device, solidifying the drug-containing biological material; and delivering the medical device to the target tissue for treating the tissue.

A method for treating vulnerable plaques of a patient, comprising: providing a biodegradable stent comprising a first supporting zone made of a first biodegradable material, wherein the supporting zone comprises at least a portion of continuous circumference of the stent; and a second therapeutic zone made of a second biodegradable material, wherein the therapeutic zone comprises at least one bioactive agent; delivering the biodegradable stent to the vulnerable plaques; orienting the therapeutic zone at about the luminal surface of the vulnerable plaque; and releasing the at least one bioactive agent for treating the vulnerable plaques.

The invention relates to food pachaging material, in particular edible packing film prepared from soybean protein isolate and glutin and its preparing method and use. The packing film is composed mainly of soybean protein isolate and glutin. Film solution for preparing the film is obtained by mixing two solutions of soybean protein isolate and glutin. The film solution contains soybean protein isolate, glutin, and further adding auxiliary haulages for improving film property, such as glycerin, crosslinking agnet (genipin,transglutaminase or alum), alkaline matter(sodium carbonate, sodium hydroxide).The film appearance is similar to plastic film, can be solved in 30-100deg.C water, and has certain nutritive value. The invention makes the best of advantage of glutin and soybean protein isolate, and obtains edible packing film with definite mechanical stength and low cost.

The invention discloses nanoparticles for encapsulating polyphenol active substances and a preparation method thereof, which belong to the technical field of high-polymer chemisty and functional foods. In the method, protein-polysaccharide nanoparticles for encapsulating polyphenol active substances through a Maillard reaction of proteins and polysaccharides and self-assembly of polyphenol and a protein-covalence conjugate. The nanoparticles have excellent properties of high encapsulating efficiency on polyphenol active substances, high loading amount, high protection activity and the like. The nanoparticles are cross-linked by using crosslinking agents such as glutaraldehyde, genipin and the like, so that the stability and slow-release performance of the nanoparticles can be further improved. A preparation process is performed in a full-water-phase environment, organic reagents, surfactants and other toxic reagents are not used, and raw materials of a carrier belong to natural macromolecules, so that the nanoparticles have excellent biocompatibility and biodegradability, and are accordant with the requirement of green chemistry.

The invention discloses a method of using acellular bovine pericardia fixed with genipin as a surgical-repair material to fix an abdominal wall defect.

A pharmaceutical microsphere comprises a bioactive agent and a biological carrier that encapsulates the bioactive agent, wherein the biological carrier is crosslinked with a crosslinking agent.

The invention belongs to the biotechnology field and relates to a preparation method of genipin. The method comprises a preparation process of immobilized enzyme, a transformation process of gardenoside and a separation and purification process of genipin and is characterized by fermenting the strains for producing beta-glucosidase, collecting the fermentation liquor after reaching the enzyme producing peak, co-immobilizing enzyme and cells or enzyme and hyphae by combining embedding with crosslinking to prepare the immobilized enzyme, utilizing a packed-bed or stirred reactor to carry out catalyzed hydrolysis on the gardenoside and obtaining genipin after purification. The invention dispenses with separation and purification of the fermentation liquor, the immobilized enzyme obtained by preparation has high activity still keeping over 50% after operation for 960h and produces less pollution to the environment, genipin has high yield and the method has obvious advantages compared with other methods.

The invention provides a production method of a natural blue pigment, which comprises genipin prepared by reaction of beta-glucosidase catalyzing gargenoside, and natural gardenia blue pigment prepared by reaction between the genipin and amino acid in a proper condition. The production method is characterized in that metalloenzyme accelerator is added in the reaction of beta-glucosidase catalyzing gargenoside. Because the metalloenzyme accelerator is added in the reaction, the speed of enzymatic reaction can be accelerated and the reaction time is shortened, so that the production cost is saved, which is helpful for industrialized production. Meanwhile, metal ions added are all the trace elements that the human body needs, so the prepared pigment not only does not have the potential harm that the artificial pigment has, but oppositely has good health function, therefore the pigment can be applied in various fields like food industry.

The invention discloses genipin cross-linked biogel as well as a preparation method and application thereof. The genipin cross-linked biogel comprises the following raw materials in percentage by weight: 0.05wt%-0.2wt% of genipin, 1wt%-10wt% of matrix and the balance of water. By carrying out crosslinking by virtue of optimized genipin concentration, the obtained biogel has the properties of low toxicity, good water-absorbing property and mechanical property and the like. The biogel can be directly used for protecting and isolating the surfaces of various wounds, bedsores and ulcers, can be used as a dressing carrier to load antibacterial drugs, paregorics, growth factors and the like, and can be applied to the treatment of wound infection, wound analgesia or promotion of wound healing and the like. According to the genipin cross-linked biogel, the matrix, a crosslinking agent and the components are all derived from natural organisms, so that the biogel is safe and non-toxic and can be widely applied to the treatment and repair of clinical acute and chronic wounds.

A method for preparing a porous-three-dimensional scaffold good for tissue engineering is described. Sericin forms a three-dimensional scaffold with PVA after freeze-drying having glycerin as a plasticizer and genipin as natural crosslinking agent to help making a strong and stable matrix. Adding glycerin into scaffold gives good uniformity and porosity. Smaller pore sizes and better uniformity are obtained as the concentration of genipin in the scaffold increases. Glycerin retains a high moisture content to allow the presence of water molecule in the matrix structure. Adding genipin results in a higher degree of crosslinking within the scaffold. Crosslinking using genipin is most beneficial in preparing scaffold possesses the best biological and physical properties for wound healing. The present invention describes method for preparing crosslinked matrix whose composition can be appropriately tuned to obtain matrix with desirable characteristics for biological applications.

Novel compositions comprising genipin for cross-linking polymer fibers, are provided. In aspects of the invention the compositions further comprise a solvent system, wherein said solvent system comprises alcohol solvent and water. The genipin-based compositions are useful in methods for promoting the stabilization of fibers in an aqueous environment, and in tissue engineering. The novel genipin-based composition is also useful in methods of treating dermatological conditions.

The process of preparing gardenia yellow pigment includes the following steps: 1. simultaneous water extracting and enzymolysis to obtain extracted solution; 2. adding calcium salt into the extracted solution to clarify, centrifuging and ultrafiltering to obtain filtrate; and 3. macroporous resin adsorption of the filtrate, three-section eluting chromatography, concentrating, and drying to obtain gardenia yellow pigment with high color number and low jasminodin content. The process is simple and suitable for use in industrial production and can eliminate jasminodin chlorogenic acid effectively, and the gardenia yellow pigment product has A238 / A440 not higher than 0.4, A325 / A440 not higher than 0.35, color number of about 400 and stable quality.

The invention discloses a plant starch-chitosan composite packaging film and a preparation method thereof, and relates to the technical field of packaging films. The plant starch-chitosan composite packaging film comprises raw materials in parts by mass as follows: 90-100 parts of plant starch, 50-60 parts of chitosan, 25-30 parts of rhizoma coptidis, 20-25 parts of rosemary, 15-20 parts of konjak powder, 15-18 parts of sodium alginate, 12-15 parts of polyvinyl alcohol, 12-15 parts of epoxidized soybean oil, 10-12 parts of low-methoxy pectin, 8-10 parts of calcium citrate, 8-10 parts of curdlan, 6-9 parts of edible gelatine, 5-8 parts of genipin, 4-6 parts of polyglycerol fatty acid ester, 3-5 parts of edible essence and 1,000-2,000 parts of water. According to the plant starch-chitosan composite packaging film and the preparation method thereof, the plant starch and the chitosan are taken as main raw materials, and the production cost is reduced; various coalescing agents with edible safety and an extracting solution of a Chinese herbal medicine rhizoma coptidis and rosemary are added, so that the prepared packaging film has better physical and mechanical property, barrier property and anti-oxidation function and can be used for packaging various kinds of food.

The invention discloses a method for preparing biomimetic artificial bone materials for biodegradable tissue engineering. The method comprises the following steps: adding a hydroxyapatite suspension to a human-like collagen solution; adding Genipin for crosslinking; performing vacuum freeze-drying to obtain human-like collagen / hydroxyapatite powder; adding the human-like collagen / hydroxyapatite powder to a chitosan solution; using an NaOH solution to adjust the pH of the solution to 7.0; repeatedly adding the human-like collagen solution and the chitosan solution; adding a Genipin solution for crosslinking; freeze-drying a mixed solution; inoculating the obtained material with BMSCs of which the cell density is 5*104 cells / cm2; performing culture for 1 to 10 days; using an induction medium to culture BMSCs cells so as to induce the BMSCs cells to differentiate towards an osteogenesis direction; and forming bone repair composite materials with bone inducing activity. The artificial bone materials prepared by the method are excellent in bone inducing activity and immune compatibility, thereby thoroughly putting an end to inevitable viral hidden trouble of animal collagen scaffold materials and greatly improving use safety.

The invention discloses a preparation method of a rice mate rich in crocin and geniposide, particularly relates to a preparation method of a rice mate rich in crocin and geniposide by utilization of a subcritical extraction technology, and belongs to the field of medicinal food and healthcare food. The preparation method includes three steps, namely, degreasing, desolventizing and cooking. More than 99.9% of liposoluble constituents in materials can be extracted by the subcritical extraction technology (with the oil content of dregs after the degreasing step being lower than 0.1%). The product can be directly used or be further processed as a golden rice mate to prepare jasmine golden rice, and the like. In addition, the biological activity of functional components in the extract is guaranteed and the product is liable to store. Effective components which are the crocin and the geniposide are preserved in the dregs can be further widely applied. Comprehensive utilization of nutritions in the "dregs" after fat is extracted is guaranteed. The method has a wide application scope.

The present invention relates to medicine technology, and is especially the preparation process of preparing high purity jasminodin and genipin-1-beta-D-gentiobioside through separating and purifying cape jasmine fruit. The preparation process includes soaking or refluxing cape jasmine fruit with water or water solution of ethanol to obtain coarse extract; eluting in macroporous adsorbing resin first with great amount of water and then with low concentration ethanol solution; decompression concentrating to obtain the macroporous adsorbing resin extract of cape jasmine fruit; and final high speed liquid phase chromatographic and preparing liquid phase chromatographic separation to purify and obtain high purity jasminodin and genipin-1-beta-D-gentiobioside. The preparation process of the present invention has high separation efficiency, high product purity and simple operation, and is suitable for industrial production.

The invention discloses autofluorescence bone repairing magnetic sustained-release microspheres and a preparation method thereof. The method comprises the steps of synthesizing nano Fe3O4 by a solvothermal method; uniformly dispersing nano Fe3O4 into a sodium alginate solution while combining with a traditional Chinese medicine-icariin with proliferation and differentiation effects on osteoblast; enabling the mixture to pass through a microcapsule forming device, and dropwise adding the mixture into a gel bath to form primary microspheres; soaking the primary microspheres in a chitosan solution to form a polyelectrolyte semipermeable membrane by use of the charge complementation feature of chitosan and sodium alginate; crosslinking the composite microspheres with genipin to obtain the autofluorescence bone repairing magnetic sustained-release microspheres. According to the autofluorescence bone repairing magnetic sustained-release microspheres and preparation method thereof disclosed by the invention, the requirements of an implanted bone repairing material on cytotoxicity are taken into full consideration, the chitosan and sodium alginate with excellent biocompatibility and degradability are selected as raw materials, and the microspheres with different particle sizes can be prepared by adjusting the voltage according to the needs of the bone lesion.

The invention provides a preparation method for a nano polypyrrole chitin nerve conduit. The preparation method comprises the following steps: dropwise adding a pyrrole monomer and an initiator into an emulsifier aqueous solution for reaction, adding acetone for stopping the reaction, washing away the initiator and an emulsifier, and separating out precipitates-nano polypyrrole by centrifugation; dissolving nano polypyrrole and chitosan in acetic acid separately to prepare a nano polypyrrole and acetic acid suspension liquid and a chitosan and acetic acid suspension liquid, mixing and stirring to obtain a mixed suspension liquid, injecting the mixed suspension liquid into a mould, performing freeze-drying forming to obtain semi-solid gel, performing neutralization forming in a sodium hydroxide aqueous solution, and drying to obtain a nano polypyrrole chitosan nerve conduit; and soaking the nano polypyrrole chitosan nerve conduit in an acetic anhydride and methanol solution for reaction, soaking a reaction product in a sodium hydroxide solution, performing crosslinking by using genipin, flushing a crosslinked product with double distilled water, and performing dehydrating and drying to obtain the nano polypyrrole chitin nerve conduit. The nano polypyrrole chitin nerve conduit prepared with the method has good conductivity and controllable degradability.

The invention relates to a medical material for surgery, in particular to a chitosan polymer capable of being used as a medical bioengineering scaffold and a carrier carrying active growth factors and a preparation method thereof. In the preparation method, the raw materials comprise natural chitosan powder, gelatin powder, genipin solution and glycerol-2-disodium phosphate powder; the preparation method comprises a preparation stage of the raw materials before synthesis and a synthesis stage; and in the synthesis stage of the chitosan polymer, the natural chitosan is subjected to chemical cross-linking and ion cross-linking with the genipin and the glycerol-2-disodium phosphate respectively, and the gelatinous chitosan polymer is obtained. The method is simple in operation, the adopted materials are natural and nontoxic and are conveniently obtained, and the chitosan polymer produced by using the method has good stability and can be used as the medical bioengineering scaffold and thecarrier carrying the active growth factors.

The present invention relates to a drug-loaded biodegradable stent and methods for treating vulnerable plaques of a patient comprising a plurality of layers or zones, each layer or zone comprising its own specific biodegradation rate and its specific drug loading characteristics. In one embodiment, the layers and zones are configured and arranged, in combination, radially, circumferentially and longitudinally.

The invention discloses a gelatin porous material prepared by using a high inner phase emulsion as a template and a preparation method of the gelatin porous material. A gelatin solution and an organic solvent are stirred and mixed at a high speed to form an oil-in-water type (O / W) high inner phase emulsion, and a continuous phase is crosslinked by using genipin to form a three-dimensional network-state structure, and the three-dimensional network-state structure is frozen and dried to prepare the porous material with a pore diameter of 10-100 mu m and a porosity of more than 70 percent. The gelatin porous material prepared by adopting the preparation method is easily formed, is good in biocompatibility, and can be widely applied to a tissue engineering material.

The invention relates to a production method of a highly stable and biodegradable double-network oil-water separation net membrane. A copper net with the surface having a nanosheet rough structure after oxidation treatment is adopted as a substrate support material, natural polyose substances sodium alginate and chitosan having the advantages of high hydrophilicity, good salt resistance and biodegradability are used as main body glue-forming materials, and an inner layer chitosan network is partially crosslinked by a natural biological cross-linking agent genipin to make the inner layer chitosan network fixed on the copper net substrate and keep the flexibility of the network structure of the chitosan; and an outer sodium alginate network is physically cross-linked and fixed by adopting divalent calcium ions, and the inner layer and the outer layer are self-assembled by electrostatic force and hydrogen bonds to form a double-layer network structure. The oil-water separation net membrane with the special double-network structure has the advantages of high oil-water separation efficiency, good resistance to oil stains, acids, alkalis and high-salt environments, high stability and good durability; and additionally, a coating layer formed by the net membrane is green and environmentally friendly, and is completely degradable.

The present invention relates to a method for treating abnormal polyglutamine-mediated diseases comprising: administering a pharmaceutical composition comprising one or more selected from the group consisting of a Gardenia jasminoides extract, geniposide and crocin.

The invention relates to a method used for preparing high-purity gardenoside and crocin from gardenia jasminoides ellis, and belongs to the technical field of food and medicine. The method comprises following steps: a, gardenia jasminoides ellis is collected, and is extracted at a temperature of 40 to 70 DEG C, an obtained extracted solution is cooled to room temperature, active carbon or diatomite is added, and an obtained mixture is filtered and concentrated so as to obtain an extractum; b, the extractum is dissolved in water, pH value is adjusted to 7.8 to 9, an obtained solution is processes by using a MCI gel chromatographic column, is allowed to stand, and is subjected to elution with water, an obtained mixed material is subjected to elution with an ethanol solution with a mass percent concentration of 5 to 30% so as to obtain an eluent containing gardenoside, and an obtained product is subjected to elution with an ethanol solution with a mass percent concentration of 60 to 90% so as to obtain an eluent containing crocin; c, the eluent containing gardenoside is concentrated, is dissolved in ethyl acetate or absolute ethyl alcohol, and is subjected to crystallization so as to obtain high-purity gardenoside; the eluent containing crocin is concentrated and dried so as to obtain high-purity crocin. Beneficial effect of the method is that: purity of gardenoside and purity of crocin are both high.

The invention provides a preparation method of nanoparticles with a photothermal effect and an application thereof. The nanoparticles are prepared by the following steps: positively charged molecules such as chitosan and the like undergo a polymerization reaction triggered by the use of genipin in a reversed-phase microemulsion; the surface is PEG-modified to enhance biocompatibility; and finally, a photothermal conversion material is supported under the positive and negative charge effect. The obtained nanoparticles have remarkable photothermal effect, can be applied in photo-thermal therapy of tumor cells, and can kill tumor cells by strong photo-thermal killing capability and inhibit tumor growth.

The invention discloses a preparation method of an antimicrobial and anti-degradable small instestinal mucous accellular substrate. The preparation method comprises the following steps of 1) carrying out crosslinking on soaking genipin and small instestinal mucous accellular substrate, and then rinsing and drying; 2) transferring the small instestinal mucous accellular substrate which is crosslinked with genipin in the step 1) into a dopamine solution to be soaked, and then rinsing and drying; and 3) transferring the small instestinal mucous accellular substrate processed in the step 2) into a silver nitrate solution to be soaked, and then rinsing and drying. The invention also discloses application of the antimicrobial and anti-degradable small instestinal mucous accellular substrate in tissue repair of a high stress part. According to the preparation method, the small instestinal mucous accellular substrate is modified in antimicrobial and anti-degradable performances; the small instestinal mucous accellular substrate prepared by using the method has the antimicrobial and anti-degradable performances superior to those of a natural small instestinal mucous accellular substrate and also remains high biocompatibility, and thus the small instestinal mucous accellular substrate can be applied to the issue repair of the high-stress part.