244 results about "Genipin" patented technology

The invention relates to the technical field of medical materials, and particularly discloses a biocompatible hydrogel microneedle nursing dressing and a preparation method thereof. A biocompatible hydrogel microneedle nursing dressing comprises a hydrogel substrate, microneedles regularly attached to the side, facing the skin, of the hydrogel substrate, and a breathable film layer attached to the side, back to the skin, of the hydrogel substrate. The micro-needle array tip area of the biocompatible hydrogel micro-needle nursing dressing provides rigid support through silk fibroin, it is ensured that a micro-needle can penetrate through the cuticle, the silk fibroin and hyaluronic acid are compounded through the middle-layer needle body transition area, the mechanical strength and the swelling property are balanced, the flexibility is improved through glycerinum, the needle body is prevented from being broken, and the biocompatibility is improved. The closed area of the top substrate adopts high-concentration hyaluronic acid to achieve rapid water absorption swelling and promote drug diffusion, polydopamine nanoparticles enhance antibacterial property and tissue adhesion, genipin crosslinking improves mechanical strength, and a sealing barrier is formed to prevent drug leakage.

A material for producing macromolecule air fibers, a preparation method, and an application thereof are provided. The material for producing macromolecule air fiber is prepared by preparation of doped antibacterial TiO2 / ZnO / ZrO2 ceramic powder, deposition of magnetic iron oxide, surface modification with tannic acid, addition of water-soluble chitosan solution, immersion in a spined TPEE air fiber membrane, uniform addition of Genipin solution, heating and reacting, membrane removal, washing, and drying. The TPEE air fiber membrane produced by the present disclosure has good mechanical properties, and modified ceramic powder is uniformly dispersed in the TPEE air fiber membrane, which plays a good role in antibacterial, environmental protection, immune regulation, magnetic therapy, far-infrared heating and other effects, rendering the material for producing macromolecule air fibers have better comprehensive performance and a good mattress support material with broad application prospects.

The invention belongs to the field of medical instrument coloring agents, and provides a natural anthocyanin endoscope coloring agent. According to the invention, the innovative design of combining a ternary color-stabilizing compound formed by anthocyanin, a co-chromophore and hydroxypropyl beta cyclodextrin with a chitosan sodium alginate polysaccharide system and a composite buffer system is adopted, and the viscosity is controlled to be 200-320 millisecond, the particle size of spray droplets is 40-60 microns, and the thickness of a thin film is 10-30 microns through specific molar ratio compatibility and a genipin crosslinking technology. Through accurate regulation and control, the excellent performance that a double-network thin layer is formed under the calcium ion environment that the absorbance retention rate at 520 nanometers is not lower than 90% and the elution rate is not lower than 90% under the irradiation of hundred thousand lux white light is achieved, the technical problems that a traditional endoscope coloring agent is poor in safety, low in stability and insufficient in mucous membrane contrast ratio are solved, and the wide clinical application value is achieved.

The invention discloses a preparation method of antibacterial and hemostatic medical sponge, and relates to the technical field of preparation of medical sponge. The preparation method of the antibacterial hemostatic medical sponge comprises the following steps: cleaning silkworm cocoons after removing impurities, and immersing the cleaned silkworm cocoons in a neutral protease solution to complete degumming so as to obtain fibroin fibers; the preparation method comprises the following steps: dissolving chitosan in an acetic acid solution, dissolving polyvinyl alcohol in deionized water, adding sodium alginate, adding a silk fibroin solution to form a composite solution, dropwise adding an AgNO3 solution, introducing CO2, maintaining the pressure, quickly releasing the pressure, foaming, injecting into a mold, carrying out gradient freezing to form a multistage-aperture sponge matrix, immersing in a genipin ethanol solution, and transferring into a 5% CaC 12 solution for soaking. The silk fibroin provides a biocompatible substrate and promotes cell adhesion, the sponge is constructed into a hierarchical pore structure with a compact surface and a loose interior, and liquid absorption and blood coagulation factor enrichment are accelerated.

The embodiment of the invention relates to the technical field of electrolytes, and provides a preparation method of a hydrogel electrolyte, the hydrogel electrolyte and a zinc ion battery, and the preparation method comprises the following steps: preparing a chitosan aqueous solution with a first preset concentration; the preparation method comprises the following steps: adding a certain amount of genipin into a chitosan aqueous solution, so that the genipin and chitosan in the chitosan aqueous solution are crosslinked at a first preset temperature; coating the cross-linked slurry on a substrate of a preset material, and drying and demolding at a second preset temperature to obtain a hydrogel electrolyte dry film; and soaking the hydrogel electrolyte dry film in a liquid electrolyte with a second preset concentration to absorb the liquid until the liquid is saturated, thereby obtaining the hydrogel electrolyte. Chitosan and genipin used in the embodiment of the invention are natural substances and are non-toxic and harmless, so that the prepared hydrogel electrolyte belongs to an environment-friendly electrolyte material, the problem that a cross-linking agent in a traditional electrolyte material is relatively high in toxicity is effectively avoided, and the problem of side reaction caused by using water as an electrolyte solvent is also avoided.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention discloses an antihypertensive material for subcutaneous implantation and a preparation method of the antihypertensive material, and belongs to the technical field of biomedical materials. The antihypertensive material comprises a carrier material and a drug mixture loaded on the carrier material according to a mass ratio of (10-15): (5-8), and the carrier material comprises the following raw materials: polylactic acid-glycolic acid copolymer, citric acid-chitosan ester, genipin and a silicon dioxide active material. The silicon dioxide active material is obtained by compounding mesoporous silicon dioxide nanoparticles and a collagen coating on the surface of the mesoporous silicon dioxide nanoparticles. According to the antihypertensive material disclosed by the invention, a degradable skeleton is provided by the polylactic acid-glycolic acid copolymer, citric acid-chitosan ester is used as a toughening agent and an antibacterial material, genipin is used as a cross-linking agent, and a mesoporous structure of a silicon dioxide active material is used for loading a medicine; the problems that an existing subcutaneous implant material is high in burst release rate, poor in mechanical property, insufficient in biocompatibility and the like are solved.

The invention relates to the technical field of biological medicine, and particularly provides visual hydrogel based on multiple response mechanisms as well as a preparation method and application of the visual hydrogel. The preparation method comprises the following steps: (1) preparing an inclusion compound from beta-cyclodextrin, hydroxypropyl-beta-cyclodextrin and anthocyanin; (2) respectively preparing a polyvinyl alcohol / rhein solution and a mixed solution of quaternary ammonium salt chitosan / hyaluronic acid / kappa-carrageenan; and (3) initiating dynamic crosslinking through phenylboronic acid, constructing a multi-network structure by cooperating with tannic acid, the clathrate compound and genipin, and performing gradient temperature control curing to form the hydrogel. The hydrogel has the pH / temperature / ROS response characteristic, the wound surface microenvironment state can be fed back in real time by means of anthocyanin color change, meanwhile, hyaluronic acid / polyvinyl alcohol can construct a 3D microchannel structure, ordered arrangement of collagen is promoted, wound surface healing is accelerated, and the wound surface healing effect is improved. Therefore, the problems that direct loading of anthocyanin is unstable, and a single response mechanism is difficult to adapt to a complicated microenvironment of a diabetic wound surface are solved.

The invention relates to the technical field of functional food and biological medicine, in particular to a modified pseudo-ginseng exosome and an intestinal conditioning gel composition and application thereof. According to the gel beverage, an exosome derived from panax notoginseng callus cultured in a laboratory is used as a core active component, is modified through phosphatidylserine-aminated pectin-genipin and is embedded into a hydrogel matrix composed of carboxymethyl chitosan and sodium alginate, so that the exosome is protected from being degraded in the stomach, and the activity of the exosome in the stomach is improved. The exosome can be effectively released under the intestinal pH and flora environment, so that the targeted delivery of the intestinal tract is realized, and the preparation method is suitable for improving the intestinal inflammation and maintaining the intestinal health. The invention can effectively solve the technical problems of uncontrollable raw material quality and safety, low oral delivery stability and active ingredient availability and poor medication compliance of the existing plant exosome.

The invention belongs to the technical field of water treatment materials, and particularly discloses chitosan-polyvinyl alcohol photo-thermal aerogel as well as a preparation method and application thereof. Chitosan, polyvinyl alcohol and genipin are cross-linked to prepare the chitosan-polyvinyl alcohol photo-thermal aerogel, and the chitosan-polyvinyl alcohol photo-thermal aerogel has the advantages of being simple in preparation method, low in cost, low in energy consumption, high in light absorption rate, high in evaporation rate, good in salt resistance and stability, capable of self-floating and the like; meanwhile, the hydrophilic advantages of the chitosan and the polyvinyl alcohol are combined, the hydrophilic performance is remarkably enhanced, the efficient evaporation performance is shown, the purpose of efficient evaporation can be achieved, and the chitosan / polyvinyl alcohol composite material has huge potential application prospects in the fields of seawater desalination, sewage treatment and the like.

A natural aldehyde-functionalized all-biomass aerogel and a preparation method thereof, the method comprises: (1) dispersing chitosan in a starch-like fiber solution and stirring to obtain a uniform mixed solution; (2) adding genipin as a crosslinking agent to the mixed solution obtained in step (1), heating and stirring for a period of time to obtain an aerogel precursor solution, and allowing the same to stand to undergo crosslinking to obtain a hydrogel; (3) freezing the hydrogel obtained in step (2), and then freeze-drying to obtain an aerogel; (4) soaking the aerogel obtained in step (3) in a natural aldehyde solution for graft modification, then removing and rinsing, and subsequently drying to obtain the aldehyde-functionalized aerogel.

The invention relates to the field of cable materials, and particularly discloses a high-strength fireproof cable and a preparation method thereof.The high-strength fireproof cable comprises a conductor, an insulating layer, a flame-retardant layer and an outer sheath; the outer sheath material comprises the following raw materials: modified polyamide-imide resin, linear low-density polyethylene, amino modified carbon fiber, a styrene-maleic anhydride copolymer and genipin; the preparation method comprises the following steps: firstly, heating and mixing the modified polyamide-imide resin, the amino modified carbon fiber and the genipin, and then adding other raw materials for melt blending granulation to prepare the outer sheath; and sequentially assembling the conductor, the insulating layer, the flame-retardant layer and the outer sheath to obtain the high-strength fireproof cable. The fireproof cable has the advantages that the mechanical strength of the fireproof cable is kept under the long-term high-temperature condition, and the mechanical strength and the thermal aging performance of the cable are improved.

The invention relates to a preparation method of an insect-proof lyocell fiber, and belongs to a functional fiber preparation method in the chemical field. The invention provides a bio-based microcell barrier packaging solution aiming at three major defects of easy breakage, non-environmental response release and toxicity of a chemical cross-linking agent in the existing microcapsule coating. The pH response type microcapsule is constructed through double-layer alternate self-assembly of chitosan and phytic acid, a compact network barrier is formed by electrostatic crosslinking of positive and negative charges, and the structural stability is enhanced by combining a natural cross-linking agent genipin; in the fiber loading stage, plasma pretreatment is adopted to increase the surface roughness, vacuum negative pressure drives microcapsule permeation, and steam setting promotes anchoring setting. Core innovations include an intelligent release mechanism, washability breakthrough, ecological safety and actually measured performance display. The method solves the problem that the lyocell fiber is difficult to decompose the permethrin in the NMMO solvent system, and has the advantages of efficient protection and environmental friendliness.

The invention discloses a biochar-reinforced hydrogel slow-release fertilizer and a preparation method thereof, and relates to the technical field of fertilizers. Comprising the following steps: S1, preparing sorghum straw biochar; s2, adding the sorghum straw biochar and a potassium nitrate and ammonium dihydrogen phosphate solution into a polymer monomer mixed solution to obtain a biochar gel mixed solution; s3, adding the genipin solution into the biochar gel mixed solution, stirring, performing ultrasonic treatment, freezing and unfreezing to obtain the biochar reinforced hydrogel slow-release fertilizer. Aiming at the technical defects of the hydrogel slow-release fertilizer, the performance of the hydrogel slow-release fertilizer is modified and enhanced by utilizing the biochar, and the biochar-enhanced hydrogel slow-release fertilizer is formed. The preparation method is simple in process and convenient to operate, the raw materials are cheap and easy to obtain, the cost is low, and the prepared biochar reinforced hydrogel slow-release fertilizer has the advantages of good slow-release performance, high water retention capacity and environmental stability, and is of great significance in popularization and application of the hydrogel slow-release fertilizer.

The invention provides a rutin-modified soybean protein isolate-pectin-genipin nano-particle carrier and a preparation method of the rutin-modified soybean protein isolate-pectin-genipin nano-particle carrier. The preparation method of the nanoparticle carrier comprises the following steps: preparing a soybean protein isolate solution, a soybean protein isolate solution after pH shift, an aqueous solution containing rutin-modified soybean protein isolate and an aqueous solution containing rutin-modified soybean protein isolate-pectin; the aqueous solution contains rutin-modified soy isolate protein-pectin-genipin, the concentration of the genipin is 0.05 mg / mL, and the rutin-modified soy isolate protein-pectin-genipin nano-particle carrier is prepared from the rutin-modified soy isolate protein-pectin-genipin nano-particle carrier. The invention further provides application of the rutin-modified soy isolate protein-pectin-genipin nanoparticle carrier. The rutin-modified soy isolate protein-pectin-genipin nanoparticle carrier is used for sustained release of gastric drugs and targeted release of drugs in small intestines. According to the preparation method disclosed by the invention, a rutin-modified soybean protein isolate-polysaccharide nanoparticle carrier with a shell-core structure can be formed, gastric acid erosion can be effectively resisted, and the rutin-modified soybean protein isolate-polysaccharide nanoparticle carrier is delivered to small intestines in a targeted manner.

The invention discloses a preparation method of gardenia blue pigment. The preparation method comprises the following steps: (1) precipitating and removing impurities by copper sulfate: precipitating and removing impurities from column liquid after gardenia yellow is extracted by copper sulfate to obtain supernate; (2) oxidizing and decoloring by potassium permanganate and removing excessive copper ions: performing neutral oxidation on the supernate by potassium permanganate to obtain decolored supernate; (3) nanofiltration concentration and spray drying: carrying out alkaline nanofiltration concentration and spray drying on the decolorized supernatant to obtain a geniposide solid crude product; (4) extracting geniposide with propyl alcohol and recrystallizing: extracting the crude product with ethyl acetate and recrystallizing to obtain a fine geniposide product; (5) ethyl acetate two-phase acid hydrolysis: carrying out ethyl acetate two-phase acid hydrolysis and recrystallization on the refined product to obtain a genipin pure product; and (6) preparing a gardenia blue pigment pure product: reacting the genipin pure product with sodium isoleucine to obtain the gardenia blue pigment. The method is simple and easy to implement, convenient to operate, outstanding in product characteristics, easy in equipment type selection and matching, low in equipment investment, high in product additional value, good in economic benefit and capable of being mechanized.

The invention discloses a tetravalent influenza virus subunit vaccine and a preparation method thereof, and relates to the technical field of biology, each dose of the tetravalent influenza virus subunit vaccine comprises two influenza A viruses and two influenza B viruses, the tetravalent influenza virus subunit vaccine is prepared by virus inoculation, virus multiplication culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, splitting and ultracentrifugation purification, mixing, filtration sterilization, split charging and packaging. Inactivation comprises the following steps: adding a cross-linked carboxymethyl glucan solution into the monovalent virus harvesting liquid, adding formaldehyde, and inactivating at 5 DEG C for 45 hours; then genipin is mixed into the virus liquid, the stirring speed is 200 rpm, the stirring time is 8 h under the conditions that the pH is 7.2 and the temperature is 25 DEG C, residues of free methanol can be further reduced, migration of small molecules is inhibited, and the long-term storage stability is guaranteed.

The invention relates to a dressing for promoting healing of diabetic wounds. Chitosan is dissolved in a hydrochloric acid solution at room temperature to obtain a chitosan solution; the preparation method comprises the following steps: dissolving loxoprofen in ethanol activated by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide / N-hydroxysuccinimide, dropwise adding into a chitosan solution, adding ethanol while stirring at a high speed by using a magnetic rod to form a transparent solution, and stirring at a low speed in the dark; air-drying the solution in a drying oven to form a solid film, and thoroughly washing the solid film in ethanol to obtain a chitosan-loxoprofen conjugate; the preparation method comprises the following steps: adding chitosan-loxoprofen into a phosphate buffer solution to form a chitosan-loxoprofen solution; dissolving genipin in a phosphate buffer solution to obtain a genipin solution, stirring and mixing the chitosan-loxoprofen solution and the genipin solution at a high speed, cross-linking, and freezing in a refrigerator to obtain the chitosan-loxoprofen / genipin sponge bracket. The dressing disclosed by the invention can effectively relieve chronic inflammation, remarkably improve skin tissue regeneration and stimulate angiogenesis.

The invention discloses a microbial soil conditioner and a preparation method thereof, and relates to the technical field of soil improvement. The method comprises the following steps: culturing heavy metal-resistant chlorella and aspergillus niger to obtain a mixed bacterial suspension, combining the mixed bacterial suspension with a composite chelating modifier prepared by reacting chitosan quaternary ammonium salt, ethylenediamine tetraacetic acid and epichlorohydrin, carrying out crosslinking by using genipin to obtain a functional microbial agent, and carrying out pyrolysis and acid oxidation on corn straws as a raw material to obtain the heavy metal-resistant chlorella modified functional microbial agent. The preparation method comprises the following steps: preparing a nano zero-valent iron-biochar composite material, reducing and loading ferrous ions through sodium borohydride, preparing the nano zero-valent iron-biochar composite material, mixing the nano zero-valent iron-biochar composite material with zeolite, bentonite and a silane coupling agent, carrying out spray drying to prepare a porous carrier substance, and finally stirring and mixing an organic fertilizer complex microbial inoculant, the functional microbial inoculant and the carrier substance, and aging to prepare the soil conditioner. The prepared conditioner has a good remediation effect on soil heavy metal pollution.

The invention relates to the technical field of 3D cell culture materials, in particular to a preparation method of a 3D cell culture material, which comprises the following steps: S1, raw material preparation: raw materials comprise sodium alginate, chitosan, polyvinyl alcohol and a phosphate buffer according to a mass ratio of 1: (0.6-0.8): (0.1-0.3): (1-3). According to the invention, the porous structure is also beneficial to enhancing the mechanical properties of the material, after the chitosan is pretreated, the molecular chain of the chitosan can be stretched, the compatibility with other components is better, the formation of a stable network structure is facilitated, and the gelatin and the genipin are subjected to a cross-linking reaction through ultraviolet irradiation to form a three-dimensional network structure; the cross-linked structure can improve the mechanical property of the material, so that the material has better stability and deformation resistance, and the use performance of the material is effectively improved.

The invention discloses double-network hydrogel based on chlorogenic acid as well as a preparation method and application of the double-network hydrogel, and belongs to the technical field of wound dressings. A first network of the double-network hydrogel based on the chlorogenic acid is formed by coordination of the chlorogenic acid and Zn < 2 + >, and CA and Zn < 2 + > can be quickly released in inflammation and proliferation stages, so that the effects of resisting bacteria, resisting oxidation, diminishing inflammation and generating blood vessels are achieved; the second network is formed by covalent crosslinking of heparin grafted gelatin and genipin, crosslinking of gelatin and genipin can enhance the mechanical strength, heparin is grafted into gelatin, bFGF can be stably added, the release time of bFGF at a wound part can be prolonged, and bFGF is slowly released in proliferation and remodeling stages, so that scars are prevented from being formed; in-vivo studies of MRSA infected diabetic mouse models and rabbit ear hyperplastic scar models show that the dual-network hydrogel can significantly promote healing of scar-free wounds. The programmed release function of the CZGF hydrogel plays a key role in accelerating chronic wound healing and preventing scar formation.

The invention relates to the technical field of medicines, in particular to a stable drug-loaded microcapsule and a preparation method thereof. According to the method, A-type gelatin and succinic anhydride modified B-type gelatin are adopted to synergistically construct a layered composite shell layer, genipin is added step by step to realize progressive crosslinking, and the interface structure and the controlled release performance are optimized. The microcapsule prepared by the invention has high encapsulation efficiency, controllable release and excellent storage stability, adopts an innovative multi-molecular-weight gelatin layered design and a step-by-step cross-linking strategy, and takes into account the dual requirements of strong encapsulation and slow release and controlled release, so that the vitamin B6 is prevented from being influenced by environmental degradation, and the efficacy and compliance of a preparation are improved. The invention provides a new technical scheme for development of controlled release preparations of water-soluble vitamins and related drugs.

The invention belongs to the field of polymer chemistry and biological medicine materials, and relates to application of a polymer polysaccharide slow-release carrier in medicinal and edible components and a preparation method of the polymer polysaccharide slow-release carrier. The carrier is composed of phytic acid-genipin cross-linked modified chitosan, tannic acid, sodium lactate, glycerin, vitamin E acetate, sodium alginate and deionized water. The phytic acid-genipin cross-linked modified chitosan is prepared by reacting chitosan with phytic acid and genipin, and has reversible cross-linking and polydentate coordination properties; the tannic acid and the modified chitosan can form a multi-point hydrogen bond and esterification network. The carrier is prepared through sol-gel forming or spray drying, is stable in structure, good in dispersity, high in drug loading capacity, stable in an acid environment and controllable in release under a neutral condition, remarkably improves the slow release performance and bioavailability of medicinal and edible components, and is suitable for functional food and drug delivery systems.

The invention relates to composite aerogel as well as a preparation method and application thereof, and belongs to the technical field of bio-based materials, the composite aerogel is prepared from the following components in parts by mass: 2-10 parts of aldehyde modified cellulose nanocrystals, 3-10 parts of a silk fibroin solution, 5-15 parts of a genipin ethanol solution and 0.2-0.5 part of zinc chloride; the preparation method of the composite aerogel comprises the following steps: (1) uniformly mixing aldehyde modified cellulose nanocrystals, a silk fibroin solution, a genipin ethanol solution and zinc chloride, and reacting to form hydrogel; (2) adding into a mold, and freezing at constant temperature for 4-8 hours; (3) replacing water with tert-butyl alcohol; and (4) drying at normal pressure for 24-48 hours to obtain the composite aerogel. The aerogel prepared by the invention has a hierarchical pore structure and an adjustable function, has high mechanical properties, specific surface area, porosity and degradation rate, and is suitable for the fields of biomedicine, environmental protection and energy.

The invention discloses an identification method of collagen. A reagent for identification is genipin. The method comprises the following identification steps: I, when the concentration of the collagen is higher than 0.05%, uniformly mixing the genipin solution with the collagen and / or the collagen product solution for reaction and color development, and directly comparing colors for identification; iI, when the concentration of the collagen is lower than 0.05%, after a collagen product solution is extracted, developing by using a genipin solution, and directly comparing colors for identification; and III, when the concentration of the collagen is lower than 0.05%, uniformly mixing the genipin solution and the collagen solution for reaction and color development, and directly comparing colors for identification. According to the identification method, the requirement for the range of the collagen content in collagen raw materials and products is wider, expensive equipment does not need to be used in the identification process, and the identification cost is greatly reduced; the operation process is convenient, the experiment period is short, the identification speed is high, the sensitivity is higher, the stability is good, and the collagen content identification efficiency is improved.

The invention discloses a preparation method and application of a collagen packaging film, and belongs to the technical field of bio-based food packaging materials.The method comprises the steps that animal-derived collagen is dissolved in an acetic acid solution with the pH ranging from 3.5 to 4.5 to form a solution with the concentration ranging from 3% to 5%; sequentially adding genipin accounting for 15-25% of the dry weight of the collagen and cellulose nanowhiskers accounting for 8-12% of the dry weight of the collagen, and carrying out a cross-linking reaction at 35-40 DEG C to construct a three-dimensional network structure; then soaking treatment is performed by adopting a 70-80% ethanol solution containing 0.5-1.5% of propyl gallate, and the surface hydrophobicity is improved through specific binding of phenolic hydroxyl groups and collagen hydrophobic regions; and finally, carrying out vacuum drying and 60-70% humidity environment balance treatment to obtain the degradable membrane material with high mechanical strength and moderate waterproofness. The preparation method is particularly suitable for fresh-keeping packaging of chilled meat products, and the shelf life of food can be effectively prolonged while the biodegradability of materials is kept.

The invention discloses a composition for treating intractable skin infection, sores and ulcers and inflammation as well as a preparation method and application of the composition, and belongs to the technical field of functional compositions. The composition is prepared from the following components in parts by mass: 1 to 5 parts of baicalin, 0.5 to 3 parts of antibacterial alkaloid, 2 to 6 parts of asiaticoside, 3 to 8 parts of salvianolic acid B, 1 to 4 parts of gallic acid, 5 to 10 parts of rhizoma bletillae polysaccharide, 3 to 10 parts of konjac glucomannan, 3 to 5 parts of chitosan, 8 to 20 parts of glycerol, 0.5 to 2 parts of genipin, 1 to 3 parts of calcium chloride, 1 to 3 parts of laurocapram and 40 to 80 parts of water. The antibacterial alkaloid comprises berberine or phellodendrine. Based on deep understanding of an intractable skin pathological mechanism, the components are scientifically matched, and the composition shows a remarkable synergistic effect in in-vitro antibacterial, anti-biofilm and anti-inflammatory experiments. The preparation process is simple, low in cost and suitable for industrial production.

The invention discloses a degradable electrospinning dressing, and belongs to the technical field of biological materials.The degradable electrospinning dressing is prepared by dispersing aminosilane modified FeO in a chitosan solution, adding a gelatin solution, stirring and mixing evenly, conducting gradient freezing under the action of a rotating magnetic field, conducting permeation with a PLGA solution, then conducting electrospinning, conducting crosslinking with a genipin solution, conducting washing and conducting drying. And sterilizing to obtain the degradable electrospinning dressing. According to the degradable electrospinning dressing prepared by the preparation method disclosed by the invention, an electrospinning auxiliary material is obtained through three-layer gradient electrospinning by modifying a Fe3O4 enhanced chitosan-gelatin composite base material with amino silane and combining magnetic field assistance and gradient freezing forming, a bionic fiber structure is constructed, wound exudation dynamic balance and long-acting antibiosis are realized, the optimal moisture balance of a wound part is kept, and the antibacterial property of the wound part is improved. And wound healing is effectively promoted.

The invention discloses polycaprolactone / acellular extracellular matrix composite hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological tissue engineering. The amino-modified polycaprolactone nanofiber and the fat acellular matrix are compounded in proportion for the first time, genipin is used as a cross-linking agent of the composite hydrogel, and the hydrogel of an injectable composite material is prepared. The crosslinking degree, the gelation time, the degradation performance, the swelling performance and the mechanical performance of the composite hydrogel with different proportions are studied, and the influence of the composite hydrogel on angiogenesis and fat regeneration in vivo is studied. According to the novel injectable composite hydrogel disclosed by the invention, the 1% PCL / DAT group composite hydrogel has good adipogenesis and vascularization effects, and a new thought is provided for subsequent soft tissue repair and regeneration research.

The invention belongs to the technical field of composite membrane preparation, and particularly relates to a salmon skin gelatin composite membrane with high mechanical performance and a preparation method thereof. Salmon skin gelatin, a chitosan solution, absolute ethyl alcohol, glycerin, sorbitol, montmorillonite, silicon dioxide, anhydrous sodium sulfite and a genipin acetic acid aqueous solution are used as raw materials, salmon skin gelatin protein and chitosan have good compatibility, protein molecules are fully stretched in the heat treatment denaturation process, the water absorption hydraulic radius of the protein molecules is increased, and the water absorption rate of the protein molecules is increased. The molecules interact with each other to form a compact space gel net structure; glycerol and sorbitol enhance the strength and toughness of the composite film; montmorillonite and silicon dioxide are beneficial to improving the mechanical strength of the composite film; in addition, the genipin acetic acid aqueous solution is added to modify free amino groups on salmon skin gelatin protein molecules, so that the interaction force among molecules is improved. Therefore, after the raw materials are specifically combined, synergistic interaction is achieved, and the mechanical performance of the salmon skin gelatin composite film is improved.