18 results about "Vitexin" patented technology

The invention provides a functional feed additive for relieving oxidative damage to intestinal tracts of piglets as well as a preparation method and application of the functional feed additive. The functional feed additive is prepared from the following components in parts by weight: 30 to 50 parts of plant extract, 0.5 to 2 parts of operculate cleistocalyx flower polyphenol-mesoporous silicon dioxide nanoparticles, 0.05 to 1 part of vitexin and 1 to 3 parts of sugarcane polyphenol. The product provided by the invention can relieve intestinal structure damage caused by oxidative stress of piglets and protect normal structures and functions of intestinal tracts; inflammatory factors are reduced, inflammatory response is improved, and intestinal health is maintained; the piglet diarrhea is reduced, and the production performance of piglets is improved.

This invention provides a method for increasing flavonoid production in longan embryogenic callus using exogenous quercetin or vitexin, belonging to the field of plant tissue culture. Key metabolites quercetin and vitexin were screened from extensive targeted metabolomics data of longan embryogenic callus treated with arginine and D-arginine. Quercetin or vitexin was added to the subculture medium of longan embryogenic callus, and the growth and flavonoid content of longan embryogenic callus at different treatment times were measured. The relative flavonoid production rate was calculated, thereby achieving the goal of high flavonoid production. The key metabolites quercetin and vitexin identified in this invention can significantly increase flavonoid production in longan embryogenic callus and have significant application prospects.

The invention discloses application of a CsC12RT1 gene in breeding of a new citrus variety, belongs to the technical field of gene engineering, and particularly relates to application of the CsC12RT1 gene in breeding of the new citrus variety, and the CDS sequence of the CsC12RT1 gene is as shown in SEQ ID NO.1. The invention further discloses application of the CsC12RT1 gene in breeding of the new citrus variety. According to the invention, through overexpression of the CsC12RT1 gene, the content of vicenin-2, nobiletin and / or tangeretin in citrus peel is increased, which prompts that the CsC12RT1 gene can be used as a candidate gene for breeding a new citrus variety with high content of vicenin-2, nobiletin and / or tangeretin, and a new functional citrus variety is cultivated by adopting a genetic engineering method.

The invention discloses a tetrastigma hemsleyanum C-glycosyl transferase and application thereof. The amino acid sequence of the C-glycosyl transferase comprises a sequence as shown in SEQ ID NO. 4. The key enzyme-glycosyltransferase (ThCGT) related to vitexin synthesis in radix tetrastigme is cloned for the first time, and it is verified through in-vitro experiments that the enzyme can efficiently catalyze apigenin C-8 site glycosylation reaction to generate vitexin. Furthermore, according to the invention, a tobacco transient expression system is utilized to confirm that the enzyme can also glycosylate the apigenin C-8 site in a plant body to generate vitexin. Besides, a tobacco stable expression system capable of efficiently generating vitexin is further constructed, and an efficient platform is provided for large-scale production of vitexin, so that the production cost of vitexin is expected to be reduced, the market supply is expected to be improved, and a basis is provided for wide application of vitexin in the fields of medicines, health care products, cosmetics and the like.

The invention discloses an application of hawthorn glycosyl transferase and related biological materials in biosynthesis of rhamnoside vitexin. The invention firstly discloses any one of the following proteins: A1) a protein consisting of an amino acid sequence as shown in a sequence 2; a2) a fusion protein obtained by connecting the N end or / and C end of the protein as shown in the sequence 2 with a protein tag. The invention further discloses the protein related biological material and application thereof. The key glycosyl transferase gene CpCGT for biosynthesis of rhamnovitexin is obtained from hawthorn for the first time, and it is proved that the protein CpCGT coded by the key glycosyl transferase gene CpCGT can catalyze 2-hydroxynaringenin to form a precursor compound 2-hydroxynaringenin-C-glucoside of rhamnovitexin. And the method has important significance on biosynthesis and heterologous production of rhamnoside vitexin.

This invention discloses the application of vitexin and its composition in products, wherein the vitexin and its composition can be used in the preparation of products with oil-controlling effects; the amount of vitexin added to the product is 1-10 μg / mL; the vitexin composition comprises vitexin and resveratrol, with a mass ratio of vitexin to resveratrol of 1:9-9:1; the amount of vitexin composition added to the product is 10 μg / mL. This invention has the advantage of being applicable to the preparation of products with oil-controlling effects.

The invention discloses a pesticide composition as well as a preparation method and application thereof, and relates to the technical field of nano pesticides. According to the invention, vitexin and gentiopicroside are used as synergists, and vitexin and gentiopicroside and a chlorantraniliprole-clothianidin compound are used as raw materials to prepare the pesticide composition. Through combination of the chemical pesticide preparation and the plant source synergist, synergistic interaction of multiple action targets and multiple action modes is achieved, efficient prevention and control of peanut underground pests are achieved, and meanwhile the drug resistance risk generated by using a single agent can be avoided. According to the pesticide composition with the microcapsule structure prepared by the invention, the stability of effective components can be improved; the slow release of the effective components can also be realized. In addition, vitexin and gentiopicroside are compounded to serve as the synergist, and the composition has a combined synergistic effect on improvement of prevention and control of underground pests.

This invention discloses a method for increasing the content of flavonoids and cyanidin in *Phyllostachys edulis*, belonging to the field of plant cultivation technology. This invention uses continuous irradiation of *Phyllostachys edulis* with light of specific wavelengths and intensities; light with wavelengths of 354-478 nm is the main wavelength inducing culm discoloration; when the blue light intensity exceeds 8 μmol / m², the irradiation is further enhanced. 2 At a certain temperature, the color and anthocyanin content of bamboo increased significantly. After light treatment, the contents of six flavonoids, including vitexin, isohypericin, narcissin, hyperoside, luteolinin, and luteolin, increased by 9.02, 8.79, 2.34, 8.5, 9.4, and 1.9 times, respectively; the content of cyanidin-3-O-rutin increased by 7.41 times.

The invention discloses a multi-index quality analysis method of dendrobium nobile and application of the multi-index quality analysis method. According to the invention, a high performance liquid chromatography (HPLC) method is adopted to establish a method for determining the content of Dendrobin A, xanthophyll, New Zealand vitexin and isophorone in stems and flowers of Dendrobium nobile; a method for determining the content of dendrobine is established by adopting a gas chromatography (GC) method, and a method for determining the content of large components including total polyphenols, total flavonoids, total anthocyanin, total polysaccharides and total alkaloids in stems and flowers of dendrobium nobile lindl is established by adopting an ultraviolet-visible (UV-Vis) spectrophotometric method. According to the quality standard disclosed by the invention, systematic and comprehensive quality control is carried out on the dendrobium nobile for the first time, and the quality standard not only has an important guiding significance on establishing the quality standard of the dendrobium nobile medicinal material red water in Guizhou, but also has an important guiding effect on research of a dendrobium nobile extraction process and development and quality control of related products.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for identifying species of fructus viticis and application of the method. When 1.5, the fructus viticis is single-leaf fructus viticis; when the ratio is gt; when 2.0, the fructus viticis is the three-leaf fructus viticis; when the ratio is between 1.5 and 2.0, it is determined that the sample is mixed or abnormal, the content determination method of the isoorientin and vitexin in the indexes is provided in detail, the precision, the stability and the repeatability of the method are good, and the average sample adding recovery rate is 80%-120%; through chemical verification, the identification accuracy reaches 100%, and the index has extremely high specificity on single-leaf fructus viticis; the ratio intervals of the pure single-leaf and three-leaf chastetree fruits are not overlapped, the critical value is clear, and cross misjudgment is avoided, so that the distinguishing degree of the identification index is extremely high, the ratio calculation result is stable, abnormal discrete data is avoided, and the objectivity and reliability of a verification conclusion are ensured.

The invention discloses a formula and a preparation method of a botanical insecticide. The mosquito-repellent liquid is prepared by mixing vitex negundo, radix stemonae, little pepper, orange peel essential oil, lavender laundry detergent, edible ethanol, maltodextrin and distilled water according to a specific weight percentage. The raw materials are all natural, the vitex negundo elements are selected from fruits, leaves and stems of vitex negundo, the radix stemonae is prepared by processing specific radix stemonae tuberous roots, the pungency degree of the small peppers reaches more than 3000 history Vills, and the orange peel essential oil is extracted from orange peel. The insecticide is natural and environment-friendly, can be quickly decomposed in the natural environment after being used, and reduces negative effects on the ecological environment. The components are diverse and efficient, various natural substances have a synergistic effect, and the insecticidal spectrum is expanded. The composition is high in safety, low in toxicity to human and livestock and less in pesticide residue. The preparation process is scientific, and stable product quality is guaranteed through fine treatment of raw materials, specific sequence mixing and precise control of stirring conditions. Part of the raw materials are wide in source, low in cost, convenient to use and high in cost performance, meet the green and environment-friendly agricultural development concept and have wide application prospects.

The invention discloses a preparation process of a heart-'an capsule and a fingerprint spectrum of the heart-'an capsule, and relates to the technical field of traditional Chinese medicine preparation processes, the preparation process of the heart-'an capsule comprises extraction and preparation, the extraction comprises the following steps: a, extraction and concentration; b, drying; c, crushing; d, total mixing; e, bagging the totally mixed hawthorn leaf extract, and sealing for later use; the preparation method comprises the following steps: S1, preparing materials; s2, performing granulation; s3, drying; s4, granulating; s5, total mixing; s6, filling is carried out; s7, inner packaging; and S8, outer packaging, wherein specification packaging, boxing, boxing, packaging and warehousing are conducted. According to the invention, the hawthorn leaf coarse powder is subjected to reflux extraction through 50% ethanol, and is prepared into granules for filling the capsules, so that the production process is stable, and the contents of rhamnoside vitexin, glucoside vitexin, vitexin, rutin, hyperoside and chlorogenic acid in each batch of produced heart-'an capsules are stable, therefore, the heart-'an capsules produced by the production process are stable in quality, and the quality of the heart-'an capsules is greatly improved. The medicine effect of the product can be ensured, and the product quality is improved.

The present application relates to the technical field of honey detection, and particularly relates to a vitexin, a preparation method thereof and a method for identifying vitex honey. The vitexin has a structure shown in formula 1: The present application finds a new compound, vitexin, in vitex honey through research on the vitex honey. The present application also finds that when the to-be-tested honey contains vitexin (p-hydroxybenzoic acid 1-O-gentio-bioside-5-hydroxy-3,7-diene-[2,6] cyclopentanopyranyl-3-methyl ester), or contains vitexin and new chlorogenic acid, 4-hydroxybenzoic acid, chlorogenic acid, caffeic acid, p-coumaric acid, red flower phaseolus acid, trans, trans-epiabscisic acid, cis, trans-epiabscisic acid, 5-methoxy pinocembrin, pinocembrin, kaempferol, pinocembrin, benzyl caffeate, kojic acid, galangin at the same time, the to-be-tested honey can be determined as vitex honey. The present application also provides a new method for constructing a vitex honey authenticity and quality evaluation system.

The invention discloses an HPLC characteristic chromatogram construction method of a pigeon pea leaf medicinal material, a concentrated solution, extract powder and a finished product, and belongs to the technical field of analysis and detection. The method specifically comprises the following steps: (1) preparing a reference substance solution; (2) preparing a test solution; (3) chromatographic conditions; and (4) determination. According to the method, a high performance liquid chromatography method is adopted, methanol-0. 1% formic acid is selected as a mobile phase for gradient elution, orientin, vitexin, pinocembrin monomethyl ether, pinocembrin, pigeon pea stilbene A and pigeon pea stilbene acid are used as reference substances, and the HPLC characteristic chromatograms of pigeon pea leaf medicinal materials, concentrated liquor, extract powder and finished products (the capsules for dredging collaterals and growing bones) are established. The method is good in precision, repeatability, stability and durability, the operability is high, analysis is convenient, stable and reliable, and the obtained high performance liquid fingerprint spectrum can effectively control the quality of pigeon pea leaf medicinal materials, concentrated solutions, extract powder and finished products.

The invention discloses a method for simultaneously determining the content of five flavone components in sabia parviflora. The method comprises the following steps: establishing an LC-MS (Liquid Chromatography-Mass Spectrometry)-based quantitative analysis of multi-components by single marker, simultaneously determining the contents of five flavonoid glycosides, namely rutin, isorhamnetin-3-O-neohesperidoside, kaempferol-3-O-rutinoside, vitexin-2 ''-O-rhamnoside and hesperidin, in stems and leaves of sabia parviflora, taking water (A)-acetonitrile (B) containing 0.1% formic acid as a mobile phase, and carrying out gradient elution; the method comprises the following steps: monitoring in an ESI positive and negative ion mode, detecting the wavelength of 254 nm, calculating relative correction factors of other four components by taking isorhamnetin-3-O-neohesperidoside as an internal standard, and measuring the content of the other four components. The method is simple, feasible, accurate and reliable, and the obtained result is not obviously different from that of an external standard method; the method not only can improve the detection efficiency, but also can reduce the cost, realizes content determination of multi-index components at different parts of sabia parviflora, and can be used for quality control of Miao medicine sabia parviflora.

The present application relates to the field of traditional Chinese medicine detection, in particular to a characteristic spectrum detection method and quality control method of a drug preparation of winter melon peel. The detection method (1) provided by the present application can select S-peak vitexin-2"-O-rhamnose glycoside as the internal reference peak in the fingerprint spectrum, and can determine five common characteristic peaks of the winter melon peel formula granules, and calculate the relative retention time of each common characteristic peak according to S-peak vitexin-2"-O-rhamnose glycoside. The detection method (2) selects S-peak uridine as the internal reference peak in the fingerprint spectrum, and can determine six common characteristic peaks of the winter melon peel formula granules, and calculates the relative retention time of each common characteristic peak according to S-peak uridine. The detection method (1) and / or the detection method (2) are beneficial to the comprehensive quality detection and overall quality control of the winter melon peel drug preparation, thereby helping to improve the safety and stability uniformity of the drug use.

The application discloses CsPGT1 Application of a gene in increasing content of flavonoid compounds in citrus, belongs to the field of agricultural biological genetic engineering technology, provides CsPGT1 Application of a gene in increasing content of flavonoid compounds in citrus, the application of the gene in increasing content of flavonoid compounds in citrus CsPGT1 The nucleotide sequence of the gene is shown as SEQ ID NO. 1. And a method for increasing flavonoid compounds in citrus pericarp by using the gene, comprising the following steps: CsPGT1 Cloning a citrus CsPGT1 Gene; constructing CsPGT1 Overexpression vector; transforming the overexpression vector into agrobacterium to obtain agrobacterium bacterial liquid; injecting into citrus fruits to obtain CsPGT1 Gene overexpression citrus fruits. The application provides a new selection for increasing flavonoid compounds in citrus pericarp, effectively increases the content of vitexin-2, naringin and / or miquiritin in citrus pericarp, which has great application value for cultivating functional new citrus varieties and accelerating the breeding process.

The invention relates to a method for preparing vitexin through solid-state fermentation of hawthorn leaves, and the method comprises the following steps: 1) activating bacillus pumilus by using a composite inducer to obtain an activated bacterial liquid; 2) taking hawthorn leaves as a raw material, and adding a composite inducer to prepare a solid-state fermentation culture medium; (3) inoculating the activated bacterial liquid into a solid-state fermentation culture medium, uniformly mixing, and performing fermentation treatment to obtain a fermentation product after fermentation is completed; according to the method, the compound inducer is used in multiple steps, the vitexin extraction technological process is simplified, the yield and the purity are improved, the preparation process is environmentally friendly, and the resource utilization rate is high.