45 results about "Phyllostachys" patented technology

The invention belongs to the field of edible fungus culture, and particularly relates to a method for reducing disease occurrence and increasing the yield of dictyophora rubrovolvata. According to the method, before planting, a rotary cultivator is used for ploughing soil, wood vinegar is sprayed, temperature and humidity are controlled after hyphae are put on the soil, wood vinegar is sprayed in the growth stage of bamboo eggs, and temperature and humidity are controlled until harvesting, so that the occurrence of diseases is reduced, and the yield of dictyophora rubrovolvata is increased. The invention provides a method for reducing diseases and increasing the yield of dictyophora rubrovolvata through measures of improving the pH value of soil, preventing soil caking, stimulating rapid growth of hyphae, stimulating twisting of the hyphae, reducing diseases and insect pests and the like. By means of the method, use of pesticides in the dictyophora rubrovolvata planting process can be reduced, the yield of dictyophora rubrovolvata is increased, and dictyophora rubrovolvata diseases are reduced.

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.

The invention discloses a preparation method of a moso bamboo derived hard carbon material and application of the moso bamboo derived hard carbon material in a sodium ion battery, and belongs to the technical field of new energy materials. Moso bamboo powder serves as a precursor, acid pickling pretreatment is conducted, then pre-carbonization is conducted at the temperature of 300-800 DEG C, secondary carbonization is conducted at the temperature of 1400-1500 DEG C after acid pickling purification, and the moso bamboo derived hard carbon material is obtained. And micropore closing and surface repairing are induced. By strictly limiting the technological process of low-temperature pre-carbonization, acid pickling and impurity removal and high-temperature reconstruction and hole closing, the contradiction that the biomass hard carbon material is large in specific surface area, low in first efficiency and insufficient in platform capacity is successfully solved. When being used as the negative electrode of the sodium ion battery, the composite material has the advantages of high reversible specific capacity (gt, 308mAh / g), high platform capacity ratio (gt, 53%) and high first charge-discharge efficiency (gt; the energy density and the cycling stability of the whole battery are remarkably improved, and a new way is provided for the development of a low-cost and high-performance sodium ion battery negative electrode material.

The invention belongs to the technical field of forestry information, and particularly relates to a moso bamboo forest individual tree high-precision segmentation method based on unmanned aerial vehicle and foundation LiDAR fusion. The method aims at solving the problems that in the prior art, in a complex bamboo forest scene, canopy information is incomplete, under-forest terrain and bamboo pole structure obtaining is inaccurate due to the fact that a data source is single, and over-segmentation and under-segmentation generally exist due to the fact that a moso bamboo growth mechanism is not considered in a traditional algorithm. Through multi-platform LiDAR data collaborative acquisition, point cloud accurate fusion is realized by adopting a registration algorithm with a bamboo pole vertical structure as a constraint, and a canopy-bamboo pole-terrain integrated three-dimensional model is formed. A comprehensive clustering algorithm fusing tree crown geometric attributes and a canopy growth space competition model is innovatively provided, the moso bamboo growth competition relation is quantified into dynamic constraint, the individual tree boundary is intelligently defined, the over-segmentation and under-segmentation problems in the segmentation process are effectively solved, and the moso bamboo forest individual tree parameter extraction precision and ecological rationality are remarkably improved.

The invention discloses Erwinia phyllostachys ZX-13 and application thereof in antagonism of epidemic disease pathogenic bacteria of Eupin, and belongs to the technical field of microorganisms. The invention discloses a Erwinia phyllostachys ZX-13, and the preservation number of the Erwinia phyllostachys ZX-13 is GDMCC No: 66800. The Erwinia phyllostachys ZX-13 disclosed by the invention can be used for preventing and treating the epidemic disease of the elaphus japonicus caused by the cryptophyton pseudophyllanthus of the elaphus japonicus; the Erwinia rhizosphere ZX-13 is a biological control potential strain with good antagonism effect, high control effect and good environmental safety, and has good development and application prospects.

The present application relates to the technical field of plant breeding, and in particular to a mutation breeding method for Phyllostachys edulis based on ARTP. The mutation breeding method comprises: using atmospheric room temperature plasma to treat the seeds of Phyllostachys edulis, and the parameters include: radio frequency power of 200-400 W, radiation distance of 2-3 mm, and treatment time of 8-55 minutes. The present application studies a mutation breeding method suitable for Phyllostachys edulis, and through a specific degree of ARTP mutation of the seeds of Phyllostachys edulis, the number of tillers of the seedlings of Phyllostachys edulis can be increased and the yellowing rate of the seedling of Phyllostachys edulis can be reduced under the condition of ensuring a certain seedling rate. The mutation breeding method provided by the present application can be used for breeding Phyllostachys edulis varieties with excellent traits, and has important value in the field of Phyllostachys edulis breeding.

The invention discloses a phyllostachys edulis PeAKIN3 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys pubescens PeAKIN3 gene provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence contained in the polypeptide coded by the phyllostachys pubescens PeAKIN3 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing an overexpression vector of a phyllostachys edulis PeAKIN3 gene; the constructed overexpression vector of the phyllostachys edulis PeAKIN3 gene is transformed into arabidopsis thaliana; and culturing and screening to obtain the transgenic arabidopsis thaliana strain. The invention discloses the function of the PeAKIN3 gene for the first time, and compared with a control group, transgenic arabidopsis thaliana with overexpression of the phyllostachys edulis PeAKIN3 gene shows obvious late flower phenotype, the number of rosette leaves is increased, and the flowering time is delayed, which indicates that the PeAKIN3 can prolong the vegetative growth period of the plant and postpone the reproductive growth of the plant.

The application discloses a bamboo summer sleeping mat processing equipment and a processing technology, which comprises a cutting cylinder device, a granulator, a flower inserting and punching machine, a water grinding device, a dryer, a carbonization furnace and a waxing machine; the cutting cylinder device clamps the phyllostachys pubescens by two calibration plates of the transverse calibration assembly to avoid transverse displacement of the phyllostachys pubescens, and clamps the phyllostachys pubescens by the bottom table and the top table of the radial calibration assembly to avoid radial displacement of the phyllostachys pubescens, so that the phyllostachys pubescens cannot produce displacement in the horizontal and vertical directions when entering the feeding port, the visual CCD camera in the feeding port can accurately determine the position of the bamboo joint, automatic identification of the bamboo joint is realized, and automatic cutting of the bamboo cylinder is realized, so that manual cutting of the bamboo cylinder is not needed, the water grinding device adopts two horizontal cylinder bodies for water grinding work, and the two horizontal cylinder bodies are opposite to each other, so that the movement mode of the bamboo pieces and the diamond powder in the cylinder is more complex, and the grinding efficiency is improved; the wormwood water is used instead of the dyeing agent, so that the finished product has no peculiar smell.

The utility model discloses a filter structure for processing phyllostachys pubescens vinegar, which comprises a filter cylinder and a filter element, the filter element is a net-shaped cylinder with a hollow interior and an open top, a mounting seat is arranged in the filter cylinder, and the bottom of the mounting seat is connected with a driving device for driving the mounting seat to rotate. An annular sliding groove is formed in the inner side wall of the mounting groove, an annular pressing plate is arranged in the annular sliding groove, the top of the annular pressing plate and the top of the annular sliding groove are connected through an elastic piece, a transition groove is formed in the annular pressing plate, a communicating groove is formed in the position, opposite to the transition groove, of the top of the annular sliding groove, and a fixing groove is formed in the position, staggered from the transition groove, of the bottom of the annular sliding groove. The fixing seat is mounted at the bottom of the filter element, and the fixing block matched with the fixing groove is arranged on the side wall of the fixing seat, so that the filter element can be conveniently disassembled and assembled, a user can conveniently clean or replace the filter element when the filter element is blocked or damaged, and the practicability is high.

The application discloses a cutting device for Phyllostachys nidularis, and relates to the technical field of Phyllostachys nidularis cultivation.The device comprises a rack, a soil aggregate transformation mechanism, a cutting mechanism and a controller.The soil aggregate transformation mechanism comprises a flat ring which is driven to move up and down by a first driving mechanism, and a rotating plate which is vertically arranged under the flat ring and is driven to rotate by a second driving mechanism.The rotating plate is inserted into soil and rotated, and the second driving mechanism drives the rotating plate to extrude the soil in the vertical projection of the flat ring towards the inner side of the flat ring.The cutting mechanism is driven to move in an inclined direction with the front being lower and the rear being higher.The controller is electrically connected with the first driving mechanism, the second driving mechanism and the cutting mechanism.The device can improve the water retention of soil, does not affect the drainage, and keeps the cutting hole depth consistent.

The invention belongs to the technical field of plant breeding, and particularly relates to an SSR molecular marker for distinguishing moso bamboo variants and application of the SSR molecular marker. According to the invention, 209 phyllostachys pubescens variant genome SSR molecular markers are screened out. The 209 phyllostachys pubescens variant genome SSR molecular markers and the polymorphic primers based on the molecular markers can be applied to phyllostachys pubescens variant SSR molecular marker development and DNA fingerprint spectrum construction, SSR marker site difference analysis can be performed on phyllostachys pubescens variants, a theoretical basis is provided for phyllostachys pubescens resource protection, utilization and identification, and the application prospect is broad. Different variants of the moso bamboos can be distinguished, and the lower grades of the moso bamboos can be Besides, the SSR molecular marker has the characteristics of good stability, high polymorphism and strong universality, and the SSR marker site disclosed by the invention has the advantages of convenience, rapidness and good sharing property when being used for analyzing the moso bamboo variant fingerprint spectrum.

The application discloses a bamboo straw preparation Phyllostachys edulis breaking device and a method thereof, and relates to the technical field of Phyllostachys edulis breaking. The device comprises a fixing column, a first mounting seat is arranged on the side of the fixing column close to a first mounting plate, the first mounting seat is fixedly connected to the outer surface of the fixing column, the number of the first mounting seats is circumferentially arranged as several, the inner walls of the several first mounting seats are all rotationally connected with bent rods through rotating rods, the ends, away from the first mounting seats, of the several bent rods are all provided with second mounting seats, the inner walls of the second mounting seats are rotationally connected with the bent rods through rotating rods, the outer surfaces of the several bent rods are fixedly connected with fixing rings, and the fixing rings are arranged outside the fixing column. The inner walls of the bamboo tubes are uniformly extruded through the arrangement of the several bent rods, so that the bamboo tubes are fixed outside the fixing rings.

This invention relates to the field of honeycomb structure technology, specifically to a biomimetic reinforced chiral honeycomb structure. The structure comprises multiple periodically arranged chiral honeycomb basic units, each consisting of a ring and multiple ligaments connecting the rings. At least some of the ligaments have circular biomimetic reinforcing nodes, the diameter of which is larger than the thickness of the ligament and is integrally connected to it. This invention simulates the localized reinforcement structure of the enlarged bamboo nodes in *Phyllostachys edulis* by locally setting biomimetic reinforcing nodes in the ligaments, significantly improving the bending stiffness and load-bearing capacity of the ligaments with relatively low added mass, resulting in higher plateau stress in the structure. This invention has a simple structural form, is relatively easy to manufacture, and can be extended to other bending-dominant honeycomb structures.

The utility model discloses phyllostachys pubescens vinegar production filling equipment which comprises a conveying belt, a filling mechanism, a detection sensor and a correction mechanism, the conveying belt, the filling mechanism, the detection sensor and the correction mechanism are respectively and electrically connected with a controller, and the filling mechanism comprises a filling head arranged above a conveying bag. The detection sensor is used for detecting the filling bottle conveyed on the conveying belt, when the conveying belt conveys the filling bottle to the position below the filling head, the detection sensor detects the filling bottle, the correction mechanism corrects the bottle opening of the filling bottle to the position under the filling head, and the controller controls the filling head on the filling mechanism to fill phyllostachys pubescens vinegar into the filling bottle. The rollers are driven to rotate through the first driving assembly, meanwhile, the two sets of rollers move relatively through the second driving assembly, the position of a filling bottle is corrected, the filling bottle is fixed between the two sets of rollers, and therefore the bottle opening of the filling bottle can directly face a filling head, and the phenomenon that phyllostachys pubescens vinegar is scattered out of the filling bottle in the filling process can be avoided; therefore, the filling work of the phyllostachys pubescens vinegar can be

The utility model belongs to bamboo processing technical field especially relates to a kind of automatic quantitative green scraping equipment of phyllostachys pubescens, including base and the machine table of being installed on the upper end of base, the right side of machine table top is equipped with scraper table, the through slot is opened in the scraper table, the scraper table right side outer wall is equipped with green scraping subassembly, the left side of machine table is fixed with side seat, the linear actuator is installed in the side seat bottom end. Through the top block of being installed with transverse movement on machine table, the feeding amount and advancing speed of first guide slide bar can be accurately controlled by linear actuator, first guide slide bar drives top block to be able to carry out transverse movement along first slide, top block pushes phyllostachys pubescens on machine table, phyllostachys pubescens can pass through the through slot on scraper table, let scraper clean phyllostachys pubescens surface quantitatively, accurately control the thickness and depth of green scraping, effectively solve the problem of uneven thickness of manual operation.

The invention discloses antibacterial bamboo-based fiber paper as well as a preparation method and application thereof. The preparation method of the antibacterial bamboo-based fiber paper comprises the following steps: 1) mixing sagittaria trifolia chips and moso bamboo chips for biological fermentation, adding sodium hydroxide and anthraquinone, and cooking to obtain fermented bamboo pulp; (2) adding the bamboo nano cellulose into the fermented bamboo pulp, and pulping to obtain paper pulp; 3, the paper pulp is made into paper, a cationic guar gum solution is sprayed to the surface of the paper, directional dehydration is conducted, and the antibacterial bamboo-based fiber paper.The antibacterial bamboo-based fiber paper has the advantages of being excellent in antibacterial performance, high in strength, good in flexibility and the like, the preparation technology is simple, the equipment requirement is low, and the antibacterial bamboo-based fiber paper is suitable for large-scale industrial production and application.

The application discloses a Phyllostachys edulis PeSVP gene, a coding protein and application thereof, and belongs to the technical field of plant genetic engineering.The nucleotide sequence of the PeSVP gene disclosed by the application is shown as SEQ ID NO.1, and the amino acid sequence of the coding protein is shown as SEQ ID NO.2.The application constructs a PeSVP gene overexpression vector of Phyllostachys edulis, transforms the constructed PeSVP gene overexpression vector of Phyllostachys edulis into Arabidopsis thaliana, and cultivates, screens and obtains a transgenic Arabidopsis thaliana strain with delayed flowering time.The application discloses the function of the PeSVP gene for the first time, the transgenic Arabidopsis thaliana plant has delayed flowering time after the PeSVP gene is transformed into the Arabidopsis thaliana plant, shows an obvious late-flowering phenotype, and the number of rosette leaves is increased;the expression amount of a downstream regulation gene FT is significantly reduced.The application can be used in molecular breeding of plant flowering period regulation, and has important significance for cultivating new plant germplasm with higher biological yield.

The utility model discloses a bamboo ground flat cutting device for tending bamboo forest, which belongs to the technical field of equipment for tending bamboo forest, and comprises a holding component, a connecting rod, a flat cutting component and a power supply component, the holding component is arranged at one end of the connecting rod, the flat cutting component is arranged at one end of the connecting rod far away from the holding component, and the power supply component is connected with the flat cutting component; the tail end of the foot pressing piece is further provided with a foot kicking pad. A battery block of the power supply assembly adopts a detachable design; according to the bamboo forest tending machine, efficient equipment is provided for tending work of qiongzhuea bamboo, square bamboo and other bamboo forests, the core design concept of portability and simplicity in operation is adopted, the bamboo forest tending machine can adapt to topographic conditions of different gradients, bamboo can be accurately and flatly cut from the ground, meanwhile, the bamboo forest tending machine has multiple practical functions of an adjustable handle, and the diversified requirements for tending of the bamboo forests are met.

The present invention provides rice noodles having a low GI characteristic and a preparation method thereof, the rice noodles comprise rice flour, water and nanocrystallized thin-walled cell walls, the nanocrystallized thin-walled cell walls are extracted from tabasheer, are edible, have one-dimensional characteristics, have a lateral dimension of 100 nm or less and a longitudinal dimension of 1 [mu] m or more, and wherein the nanocrystallized thin-walled cell walls have a cross-sectional dimension of 100 nm or less and a longitudinal dimension of 1 [mu] m or more. The mass content of the nano thin-walled cell wall in the rice noodles is between 0.1% and 1.5%. The invention also provides application of the nanocrystallized thin-walled cell wall as a low-GI rice noodle modifier and the low-GI rice noodle modifier.

This invention discloses a method for simulating the wild cultivation of *Dictyophora indicum* using dead bamboo resources, comprising the following steps: digging trenches downwards in the soil layer between *Phyllostachys edulis* groves; placing a water collection layer at the bottom of the trench; laying a lower filling layer composed of chopped bamboo residue on top of the water collection layer; laying a middle filling layer composed of a mixture of chopped bamboo residue and humus on top of the lower filling layer; constructing a cultivation trough along the length of the trench on the middle filling layer for *Dictyophora indicum* cultivation; laying a layer of pre-prepared culture medium in the cultivation trough, then placing *Dictyophora indicum* spawn with multiple holes punched around its perimeter in the middle of the culture medium, and then evenly scattering the mycelium from inside the spawn evenly on the surface of the culture medium; covering the middle filling layer and culture medium with an upper filling layer composed of sandy loam; and finally covering the upper filling layer with a covering layer. This invention has high resource utilization, significant economic benefits, and obvious ecological benefits, achieving long-term moisture retention and nutrient supplementation, and enabling fruiting for many years.

The application discloses a kind of InDel marker fingerprint of wild Phyllostachys annamica strain, belong to red-to bamboo cane strain detection technical field, solve the problem that existing technical means cannot accurately identify wild red-to bamboo cane strain, the construction of the InDel marker fingerprint of the wild red-to bamboo cane is by genome DNA extraction, construct amplification system and amplify genome DNA, and the electrophoresis detection of amplification product, to obtain the InDel marker fingerprint of red-to bamboo cane strain, this fingerprint is used as standard atlas, the electrophoresis band in atlas is marked as 1; Lower band is marked as-1; Two bands are marked as 2;The InDel marker fingerprint of the wild red-to bamboo cane strain obtained by the above numbering step is-1, 2, 2, 2, -1, 1, -1, 1, 2, 2, subsequent unknown red-to bamboo cane strain is compared by constructing fingerprint, and the obtained fingerprint is compared with standard atlas.

The application discloses a kind of for non-destructive fast evaluation of bamboo physical mechanics property prediction model construction method, mainly including collecting the growth information of target bamboo and the positioning information of the sample site where it is located;The bamboo culm of target bamboo is determined for physical mechanics property, and the measured physical mechanics property data is obtained;Correlation analysis is carried out on growth information, positioning information and measured physical mechanics property data, and the index significantly related to physical mechanics property is selected as model input variable;Based on model input variable and measured physical mechanics property data, the prediction model for predicting the physical mechanics property of bamboo is constructed.The application introduces regional grouping effect and spatial autocorrelation structure when establishing the correlation model of bamboo culm growth character and bamboo physical mechanics property, so that the final prediction model can non-destructively determine the growth character of bamboo, quickly evaluate the physical performance of bamboo, and then greatly improve the grading utilization and high-value processing efficiency of different quality bamboo resources.

This invention relates to a method for preparing polysaccharides from *Phyllostachys edulis* root with antioxidant and neuroprotective activities, and is a method for preparing natural drugs. The invention involves reflux extraction of pulverized *Phyllostachys edulis* root with ethanol to remove small molecules, followed by extraction with hot water reflux to obtain crude polysaccharides. The crude polysaccharides are then purified through a series of separation and purification processes, including protein removal, depigmentation, and column chromatography, to obtain four new natural polysaccharides: PPLR-W, PPLR-1, PPLR-2, and PPLR-3. The natural polysaccharides prepared by this invention lay the foundation for the development of antioxidant-related drugs and clinical research on the prevention or treatment of neurodegenerative diseases. The four new *Phyllostachys edulis* root polysaccharides possess DPPH and ABTS free radical scavenging abilities and exhibit significant neuroprotective effects against hydrogen peroxide-induced SH-SY5Y cell damage models.

The invention provides a stachys sieboldii storage method, relates to the technical field of agricultural product storage, and solves the problems of cell damage, out-of-control of microorganisms and the like in the storage of stachys sieboldii through a synergistic strategy of pretreatment, impurity removal and drying in the shade, three-stage gradient cooling domestication, vitamin C + calcium chloride + chitosan improved soil burying, dynamic temperature and humidity regulation and control in the storage period and regular ventilation. According to the method, the cell rupture rate of the stachys sieboldii is reduced by 60%-65% compared with direct freezing, the moisture retention rate is 93.98%, the Vc degradation rate is 33.93%, the hardness retention rate is 89.61%, the rotting rate is only 1.22% when the stachys sieboldii is stored for 180 days, the shelf life is prolonged to 180 days or above, no chemical residues exist, and the method is suitable for large-scale storage and supports off-season sales and deep processing of the stachys sieboldii.

The invention discloses a moso bamboo PeMT2 gene as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the phyllostachys edulis PeMT2 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the encoding protein of the phyllostachys edulis PeMT2 gene is as shown in SEQ ID NO.2. The preparation method comprises the following steps: cloning a phyllostachys pubescens PeMT2 gene to a pCAMBIA1302 plasmid, so as to obtain a recombinant vector pCAMBIA1302-PeMT2; transforming the recombinant vector into arabidopsis thaliana, and culturing and screening to obtain a transgenic arabidopsis thaliana strain. The transgenic arabidopsis strain shows an obvious late flowering phenotype, the number of rosette leaves is increased, and the flowering time is delayed. The invention discloses the function of the PeMT2 gene for the first time, and also discloses the application of the PeMT2 gene in regulating the flowering time of plants and the quantity of rosette leaves.

The application provides a Zhejiang phoebe acid planting soil and a preparation method thereof, and belongs to the technical field of planting soil. The Zhejiang phoebe acid planting soil is composed of vinegar residue, coconut husk, fruit shell, acid-modified wet garbage, acid-modified garden waste, garden soil and fine-toothed acaulospora. The application also provides a preparation method of the Zhejiang phoebe acid planting soil, which comprises the preparation of acid-modified wet garbage, the preparation of acid-modified garden waste, the extraction of acaulospora spores, the mixing of vinegar residue, coconut husk, fruit shell, acid-modified wet garbage, acid-modified garden waste, garden soil and acaulospora spores according to the weight ratio, and the preparation of the Zhejiang phoebe acid planting soil. The Zhejiang phoebe acid planting soil provided by the application can increase the survival rate of Zhejiang phoebe to 98%, and provides a powerful guarantee for the introduction of urban acid plants and the biodiversity, and provides an effective way for the resource utilization of urban organic waste.

The traditional Chinese medicine composition comprises a coprinus comatus extract and a traditional Chinese medicine extract, and the traditional Chinese medicine extract is prepared from mulberry leaves, cyclocarya paliurus leaves, lotus leaves and corn stigma; according to the invention, coprinus comatus is subjected to a sectional crushing process, the particle size range of sporocarp crushing is strictly controlled, and 20-40% of 200-400-mesh coprinus comatus fine powder and 60-80% of an extract extracted from 5-20-mesh coprinus comatus are uniformly mixed in the mixing and drying steps; it is accidentally found that the whole extraction effect on the hypoglycemic effect is better than that of coarse powder with the particle size of 5-20 meshes and fine powder with the particle size of 200-400 meshes.

The invention discloses an EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker combination and application of the EST-SSR molecular marker combination in identification of polygonatum plants, and belongs to the technical field of plant molecular biology. 49 pairs of high-polymorphism EST-SSR marker primers are developed on the basis of rhizome transcriptome joint data of three medicinal polygonatum sibiricum species, namely polygonatum kingianum, polygonatum kingianum and polygonatum cyrtonema, and 9 pairs of core marker primers are screened from the 49 pairs of high-polymorphism EST-SSR marker primers. The invention further provides a kit containing the marker combination and an identification method, a fluorescent capillary electrophoresis detection technology is adopted, six sibling species of polygonatum kingianum, polygonatum kingianum, polygonatum cyrtonema, polygonatum kingianum, polygonatum kingianum and Hubei polygonatum kingianum can be accurately distinguished at a time, and intraspecific variation of different geographical populations of polygonatum kingianum can be effectively analyzed. The marker combination is high in universality, high in polymorphism, large in detection flux and accurate and reliable in result, and an efficient molecular tool is provided for polygonatum germplasm resource identification, genetic diversity analysis and molecular assisted breeding.

The invention belongs to the field of genetic engineering and plant genetic breeding, and particularly relates to application of MAC5A protein in regulation and control of plant root development. In arabidopsis thaliana, deletion of the MAC5A gene can significantly inhibit root development; the overexpression of the Arabidopsis MAC5A gene can obviously promote the growth of the plant root system. In order to systematically verify the biological function of MAC5A in regulating and controlling root development, homologous genes MAC5A of rice and moso bamboo are further cloned and are respectively overexpressed in arabidopsis thaliana and rice. Experimental results show that the genes can significantly promote root development of receptor plants. Meanwhile, the endogenous MAC5A gene of the rice is knocked out by utilizing a CRISPR / Cas9 technology, so that the root system of the rice is shortened. The results prove that MAC5A is a key factor for regulating and controlling plant root development. On the basis, an important gene resource is provided for breeding of new varieties of plants and genetic improvement of root system characters, and the application prospect is wide.

The invention provides a phyllostachys pubescens forest fertilization method based on a bacterial degradation small-molecule fish peptide fertilizer. The method comprises the following steps: preparing a bacterial degradation small-molecule fish peptide fertilizer solution by using the bacterial degradation small-molecule fish peptide fertilizer; new bamboos are reserved before fertilization, and the density and age proportion of the standing bamboos are regulated and controlled; a square ditch fertilization method is adopted for fertilization, fertilization is carried out for the first time in late April, a micromolecular fish peptide fertilizer solution is applied firstly, compound fertilizer is applied after 3-5 days, and the fertilization amount ratio of 1-degree bamboos to 2-degree bamboos to 3-degree bamboos is 1: 1: 1; the fertilizing amount ratio of the bacterium solution micromolecule fish peptide fertilizer to the compound fertilizer for preparing the bacterium solution micromolecule fish peptide fertilizer solution is 4: 15; second-time fertilization is conducted in the first ten days of July, the micromolecular fish peptide fertilizer solution is applied firstly, compound fertilizer is applied after 3-5 days, and the fertilization amount ratio of the first-degree bamboos to the second-degree bamboos to the third-degree bamboos is 7: 9: 9; the fertilizing amount ratio of the bacterial degradation small-molecule fish peptide fertilizer to the compound fertilizer for preparing the bacterial degradation small-molecule fish peptide fertilizer solution is 9: 15. The application amount of the compound fertilizer in phyllostachys edulis forest rhizome shoot production can be reduced, the yield and quality of rhizome shoots are improved, and the ecological environment of bamboo forest soil is improved.