10 results about "Secondary cell wall" patented technology

The invention discloses an oenothera biennis lodging-resistant quality-improving yield-increasing functional fertilizer and preparation and use methods thereof, and belongs to the technical field of agricultural cultivation, the fertilizer comprises an active component A and an active component B. The active component A comprises one or more of methyl jasmonate, boric acid, monopotassium phosphate, gibberellin, water-soluble silicon, MgSO4, calcium chloride and melatonin, and the active component B comprises one or more of methyl jasmonate, boric acid, monopotassium phosphate, gibberellin, water-soluble silicon, MgSO4, calcium chloride and melatonin. The active ingredient B is choline chloride; the content of the active component A accounts for 0.5-15.5% of the total weight of the fertilizer, the biological yield, the economic yield and the oil content of the evening primrose are inspected, and when the active component A is single-component methyl jasmonate, the effect is good; when the active component A is composed of multiple components including jasmonic acid methyl ester, the substances provide nutrient elements for the cell wall of the evening primrose, stabilize the cell membrane structure and play a synergistic promotion role in secondary cell wall lignification, so that the lodging-resistant and disease-resistant characteristics of the evening primrose are improved.

The application discloses a gene for regulating stone cells of pear fruits PbTCP 13 The CDS sequence of the gene is shown as SEQ ID NO. 1, and the protein sequence encoded by the gene is shown as SEQ ID NO. 2. The gene is constructed into an overexpression vector, and the overexpression vector is injected into 'Dangshan Quli' pear fruits, so that the stone cell content and the lignin content of the injection part are significantly lower than those of a part injected with an empty control; the gene is introduced into Arabidopsis thaliana to obtain PbTCP13 transgenic Arabidopsis thaliana, and the secondary cell wall of the vessel cell of the transgenic Arabidopsis thaliana is significantly thinned, and the lignin accumulation in the stem is significantly reduced. The gene provides a new gene resource for fruit quality breeding, and is an important candidate gene for future gene engineering improvement of fruit quality breeding.

The invention discloses a gene PbTCP13 for regulating and controlling stone cells of pear fruits, a CDS (Coding Sequence) of the gene is shown as SEQ ID NO.1, and a coded protein sequence is shown as SEQ ID NO.2. The invention further discloses a gene PbTCP13 for regulating and controlling the stone cells of the pear fruits. When an overexpression vector constructed by the gene is injected into a Dangshan pear fruit, the content of stone cells and lignin is remarkably reduced compared with that of injection of a no-load control part; an overexpression vector constructed by the gene is introduced into arabidopsis thaliana, the secondary cell wall of conduit cells of the obtained PbTCP13 transgenic arabidopsis thaliana is obviously thinned, and the accumulation of lignin in stems is obviously reduced. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.

The invention discloses a gene PbMYB20 for regulating and controlling synthesis of pear fruit lignin, the CDS sequence of the gene is as shown in SEQ ID NO.1, and the sequence of a coded protein is as shown in SEQ ID NO.2. When an overexpression vector constructed by the gene is injected into a Dangshan pear fruit, the content of stone cells and lignin is remarkably increased compared with that of injection of a no-load control part; when a silent vector constructed by the gene is injected into a Dangshan pear fruit, the stone cell content and the lignin content are obviously reduced compared with those of a no-load control part; an overexpression vector constructed by the gene is introduced into arabidopsis thaliana, the secondary cell wall of conduit cells of the obtained PbMYB20 transgenic arabidopsis thaliana is obviously thickened, and the accumulation of lignin in stems is obviously increased. The discovery of the gene provides a new gene resource for fruit quality breeding, and the gene is an important candidate gene for improving fruit quality breeding in future genetic engineering.

The present application relates to the technical field of genetic engineering, and particularly relates to application of mitochondrial GPAT1 gene and mitochondrial GPAT2 gene in regulating plant secondary cell wall thickness. The present application firstly finds that the mitochondrial GPAT1 gene and the mitochondrial GPAT2 gene have the effect of regulating the plant secondary cell wall thickness, and the effect is embodied in that the mitochondrial GPAT1 gene and the mitochondrial GPAT2 gene regulate the formation of the secondary cell wall by negatively regulating the lignin and cellulose biosynthesis related genes. The results of the specific embodiments of the present application show that when the mitochondrial GPAT1 gene and the mitochondrial GPAT2 gene are deleted, the lignin and cellulose synthesis genes are up-regulated, a large amount of lignin and cellulose is accumulated in the cell wall of the Arabidopsis thaliana double mutant gpat1gpat2 seedling, the cell wall becomes thicker and harder, and the cotyledon texture is harder.

The invention provides a poplar DNA methyltransferase CMT3, a coding gene and application of the poplar DNA methyltransferase CMT3 in cultivation of improved forest plants, and belongs to the technical field of molecular breeding. The invention provides a poplar DNA methyltransferase CMT3. The amino acid sequence of the poplar DNA methyltransferase CMT3 is as shown in SEQ ID NO: 1. The poplar DNA methyltransferase CMT3 can improve the wood quality of plants through negative regulation and control, and can inhibit the plant height and ground diameter of the plants through negative regulation and control. According to the embodiment of the invention, the quality difference between a PagCMT3 gene knockout strain and wild type wood is compared, and the result shows that the knockout of the PagCMT3 gene can promote the expansion of cells and the thickening of secondary cell walls and change the property of the wood, so that an improved forest variety with thickened secondary cell walls and enlarged cells is obtained, and the quality of the wood is improved. The method is of great significance to genetic engineering breeding of woody plants.

The present application relates to a kind of gene PagBES1 for regulating poplar wood yield and wood fiber length and its application, belong to plant genetic engineering technical field;Its nucleotide sequence is as shown in sequence 3 in sequence table, its amino acid sequence is as shown in sequence 4 in sequence table.The present application is transferred into poplar PagBES1 gene, compared with wild type, the height of transgenic poplar of PagBES1 activation expression is significantly improved, ground stem is significantly thickened, the cell layer of stem vascular tissue is significantly increased, and wood fiber length and width are significantly increased, secondary cell wall is significantly deposited thickening, and the phenotype of transgenic poplar of PagBES1 inhibition expression is contrary to this, it is described that PagBES1 gene is the key regulation gene for regulating poplar wood formation, has important application value in the field of forest genetic engineering and clone forestry.

The invention provides a poplar chromatin remodeling factor DDM1, a coding gene and application of the poplar chromatin remodeling factor DDM1 in cultivation of improved forest plants, and belongs to the technical field of molecular breeding. The invention provides a populus argentea chromatin remodeling factor DDM1. The amino acid sequence of the populus argentea chromatin remodeling factor DDM1 is shown as SEQ ID NO: 1. The poplar chromatin remodeling factor DDM1 can improve the plant wood quality through negative regulation and control, and can inhibit the plant height and ground diameter through negative regulation and control. According to the application disclosed by the invention, the quality difference between a PagDDM1 gene knockout strain and wild type wood is compared, and the result shows that the knockout of the PagDDM1 gene can promote the expansion of cells and the thickening of secondary cell walls and change the property of the wood, so that an improved forest variety with thickened secondary cell walls and enlarged cells is obtained, and the quality of the wood is improved. The method is of great significance to genetic engineering breeding of woody plants.

The invention discloses a CbuPLT3a gene derived from catalpa bungei and an application of the CbuPLT3a gene in inhibition of synthesis of secondary cell walls of poplar trees. The nucleotide sequence of the CbuPLT3a gene is as shown in SEQ ID NO: 2. According to the invention, through an agrobacterium tumefaciens mediated genetic transformation method, the CbuPLT3a gene is introduced into the poplar '84K' (Popullus alba * Popullus glabullosa), so that an overexpression transgenic plant is obtained. Phenotypic analysis results show that compared with a wild type, the CbuPLT3a overexpression plant has the advantages that the thickness of a secondary cell wall is obviously reduced, the width of xylem is narrowed, and meanwhile, the plant height and the internode length of the plant are obviously reduced. The invention proves that CbuPLT3a is a gene capable of inhibiting synthesis of poplar secondary walls, and a new gene resource and a new technical strategy are provided for molecular design and breeding of forest wood quality.

The invention discloses a rice CEF7 gene and application thereof in controlling mechanical strength of stalks, grain size and salt tolerance, and relates to the technical field of agricultural biology and genetic engineering, and the CDS sequence of the CEF7 gene is as shown in SEQ ID No.1. The invention also provides an encoding protein, a recombinant construct and a recombinant host cell of the gene CEF7, and the expression quantity of the gene CEF7 is improved by using various means, so that the mechanical strength of rice stalks, the length-width ratio of grains, the appearance quality of rice and the salt tolerance of rice plants are improved. The method has the beneficial effects that by increasing the expression quantity of the CEF7 gene, the mechanical strength of rice stalks, the length-width ratio of grains, the appearance quality of rice and the salt tolerance of rice plants can be improved. Gene resources are provided for cultivation of new lodging-resistant, high-quality and stress-resistant rice varieties, and meanwhile, the method has guiding significance in research on molecular mechanisms of discussing rice secondary cell wall biosynthesis, grain size formation and rice salt tolerance formation.