Method for testing lignification degrees of plant cell walls

A measurement method and plant cell technology, which is applied in the field of confocal Raman microscopy to determine the degree of lignification of plant cell walls, can solve the problems of no research reports on the degree of lignification of plant cell walls under confocal microscopy, etc., to reduce the interference of fluorescence, reduce the Fluorescence interference, the effect of ensuring accuracy
CN102435594AInactive Publication Date: 2012-05-02BEIJING FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2012-05-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for testing lignification degrees of plant cell walls based on a confocal Raman microscopic technology. The method comprises the following steps of: testing Raman spectrums of the plant cell walls under the in-situ state by using a confocal Raman microscope to obtain Raman spectrum imaging graphs of lignin in the cell walls; and determining the content of the lignin in the cell walls according to Raman signal intensities, and determining the lignification degrees of different secondary cell walls and different form areas of fiber cell walls. The testing method is based on a pure spectrum method, samples are quickly prepared, chemical pollutants are not generated, and the information of the lignification degrees of the plant cell walls can be acquired under the in-situ state.
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Description

technical field

[0001] The invention relates to the application of Raman spectroscopy combined with confocal microscope technology in the study of lignification degree of plant cell walls, in particular to a method for measuring the lignification degree of plant cell walls by using a confocal Raman microscope with an excitation wavelength of 785nm in an in situ state. Background technique

[0002] Lignocellulosic raw materials are considered as fuel and chemical resources for sustainable recycling. Its cell wall is a hard layered structure similar to reinforced concrete composed of cellulose, hemicellulose and lignin. These three polymer macromolecules account for More than 90% of the total cell wall biomass. Among them, lignin is a complex and irregular polyphenolic polymer existing in vascular plants, which enhances the structural integrity of cell walls and also inhibits microbial corrosion of cell walls. The complexity of its structure and function has always been the b...

Claims

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