43 results about "Raman microscope" patented technology

A strain measurement apparatus (100) for measuring mechanical strain in a sample (1) comprises a sample holder device (10) being arranged for accommodating the sample (1) to be investigated, an indenter device (20) including an indenter tip (21) and an actuator stage (22) carrying the indenter tip (21), wherein the actuator stage (22) is arranged for an application of a localized mechanical load along a load axis z1 via the indenter tip (21) at an indentation zone (2) of the sample (1) accommodated by the sample holder device (10), when the sample holder device (10) is in a load application position, a confocal Raman microscopy device (30) having an imaging axis z2 and being arranged for collecting at least one Raman spectrum in the indentation zone (2) of the sample (1), and a calculation device (40) being arranged for calculating at least one strain parameter based on the at least one Raman spectrum, wherein the sample holder device (10), the indenter device (20) and the confocal Raman microscopy device (30) are arranged such that the confocal Raman microscopy device (30) is capable of collecting the at least one Raman spectrum, while the sample holder device (10) is in the load application position, and the indenter device (20) and the confocal Raman microscopy device (30) are arranged such that the load axis z1 of the actuator stage (22) and the imaging axis z2 of the confocal Raman microscopy device (30) coincide. Furthermore, a strain measurement method for measuring mechanical strain in a sample (1) is described.

The invention belongs to the technical field of biology, and discloses a micro-fluidic chip for in-situ single-cell non-label imaging of an upright Raman microscope. Comprising a quartz substrate subjected to surface treatment, a detachable cell chamber, a glass sheet provided with a through hole and a gravity flow channel control system, the cell chamber is used for cell culture before in-situ cell imaging, after cell culture is completed, the cell chamber is taken off, the glass sheet is bonded with the quartz substrate, the glass sheet is used for packaging a chip, and the gravity flow channel control system is used for controlling the gravity flow channel. The gravity flow channel control system is connected with the through hole and is used for guiding fluid to flow in the chip; the micro-fluidic chip can effectively package living cells and is compatible with a forward Raman microscope, so that in-situ unlabeled Raman imaging of single cells under the microscope is realized, experimental operation is simplified, more efficient cell treatment and real-time monitoring capabilities are provided, imaging stability and accuracy are improved, and the micro-fluidic chip is suitable for large-scale popularization and application. The method is of great significance to application of drug screening, biochemical research and the like.

The invention belongs to the technical field of optical microscopic imaging, and particularly relates to a miniature coherent Raman microscope and an imaging system. The coherent Raman microscope comprises a coherent Raman excitation light path and a coherent Raman signal collection light path, the coherent Raman excitation light path sequentially comprises an excitation optical fiber, an excitation optical fiber collimating lens, a micro-electro-mechanical scanning galvanometer, a scanning lens group, a dichroscope and a finite far conjugate objective lens; the coherent Raman signal collection light path sequentially comprises a finite far conjugate objective lens, a dichroscope, a collection optical fiber collimating lens and a collection optical fiber. According to the invention, a micro-electro-mechanical galvanometer and a voice coil motor are used for scanning a sample; according to the light path design, the light path structure is greatly simplified, the collection efficiency of the signal light is improved, and good imaging quality is ensured. The miniature coherent Raman microscopic imaging system overcomes the problem that unmarked in-situ imaging cannot be achieved due to the fact that a traditional coherent Raman microscope is too large in size, meanwhile, the imaging quality equivalent to that of the traditional coherent Raman microscope is kept, and the miniature coherent Raman microscopic imaging system has wide application prospects.

The invention discloses a spiral chiral gold nanorod as well as a preparation method and application thereof, and the method comprises the following steps: (1) sequentially adding hexadecyl trimethyl ammonium bromide, cysteine and p-aminothiophenol into an anisotropic gold nanorod solution, uniformly mixing, and adsorbing; and (2) silver nitrate, chloroauric acid and ascorbic acid are added for a reaction, centrifugal dispersion is performed after the reaction is finished, and the spiral chiral gold nanorod is obtained. Dispersing the spiral chiral gold nanorod in an aqueous solution, adding phenylalanine enantiomer, dropwise adding on a clean glass slide after incubation is finished, and performing surface enhanced Raman spectroscopy test by using a Raman microscope after air drying. According to the invention, the broadband coverage of chiral response from visible light to near ultraviolet region is realized by accurately regulating and controlling the arrangement of interface molecules and the morphology of the nano structure.

The invention provides a method for in-situ detection of damp-heat aging of an underfill and a detection system. The method comprises the following steps: (1) providing an underfill sample; (2) placing the bottom filling glue sample on a temperature and humidity control sample table, wherein the temperature and humidity control sample table is combined with a confocal microscopic Raman spectrometer; (3) adjusting the parameters of the temperature and humidity control sample table, and adjusting the parameters of the confocal microscopic Raman spectrometer; (4) performing in-situ Raman spectrum characterization on a certain site of the initial bottom filling glue sample under the conditions set in the step (3), then performing damp-heat aging on the bottom filling glue sample, and performing in-situ Raman spectrum characterization on the same test site of the bottom filling glue sample at regular intervals, and analyzing the change of the characteristic peak according to the obtained Raman spectrum information so as to determine the damp-heat aging mechanism of the underfill sample. The invention provides a method for in-situ detection of real-time chemical bond change information of underfill in a damp-heat aging process.

An automated portable Raman microscope combines the simplicity, ruggedness, and selectivity of Raman spectroscopy with the sensitivity of single-particle identification. It includes a visible camera, controllable illumination, and a moving optical head that together acquire images of the sample at different focal planes and under different lighting conditions. An internal processor automatically identifies particles or other targets in these images for Raman analysis, e.g., shifted excitation difference Raman spectroscopy. The moving optical head scans the Raman pump beam across the sample to different targets. A built-in user interface displays the results of the Raman analysis superimposed on visual images of the sample. The Raman microscope's simplicity, reliability, and low maintenance make it easy to use for a wide variety of applications, including analyzing and identifying particles and residues, linking chemical and biometric evidence, and forensics analysis.

The invention provides a method and a device for measuring the transverse spatial resolution of a confocal Raman microscope, and relates to the technical field of optical measurement and micro-spectrum. The method comprises the following steps: positioning a linear edge area on a single-layer graphene sample by using a confocal Raman microscope, and preparing the single-layer graphene sample on a silicon oxide substrate; determining a linear scanning path and a scanning step length from the exposed area of the substrate to the sample area in the positioned area of the linear edge along the direction vertical to the linear edge by using a confocal Raman microscope; in the moving process of the single-layer graphene sample, determining a plurality of scanning points on the linear scanning path based on the scanning step length, and collecting a Raman spectrum signal of each scanning point; and determining the transverse spatial resolution of the confocal Raman microscope based on the Raman spectrum of each scanning point. By adopting the method provided by the invention, the accuracy of the transverse spatial resolution of the confocal Raman microscope can be improved.

A sampling device comprising: a body defining a sample receiving space; a sample collection port in communication with the sample receiving space; a sample support surface positioned within the sample receiving space; and an optical alignment and detection system, wherein the sample collection port is configured to receive a sample from a stream of material and permit delivery of the sample to the sample support surface within the sample receiving space, and wherein the confocal Raman microscope is configured to measure properties of the sample when the sample is supported on the sample support surface.

The application provides a method and device for measuring lateral spatial resolution of a confocal Raman microscope, and relates to the field of optical measurement and microscopic spectroscopy technology. The method comprises the following steps: positioning a region of a straight edge on a single-layer graphene sample by using a confocal Raman microscope, wherein the single-layer graphene sample is prepared on a silicon oxide substrate; determining a straight scanning path and a scanning step in a direction perpendicular to the straight edge from a bare region of the substrate to a sample region in the region of the straight edge which has been positioned by using the confocal Raman microscope; determining a plurality of scanning points on the straight scanning path based on the scanning step in the process of moving the single-layer graphene sample, and collecting Raman spectrum signals of the scanning points; and measuring the lateral spatial resolution of the confocal Raman microscope based on the Raman spectrum of each scanning point. By using the method provided in the application, the accuracy of the lateral spatial resolution of the confocal Raman microscope can be improved.

The invention relates to a spectrometer (10) comprising an aperture (13) configured to receive a source light beam, a first optical system configured to receive the source light beam and form a collimated light beam directed to a diffraction grating, the diffraction grating configured to receive the collimated light beam and form a diffracted light beam, a second optical system configured to form an image of the diffracted light beam on an image sensor. According to the invention, the image sensor is a CMOS sensor comprising pixels (34) arranged in N rows, the pixel rows being oriented in a direction inclined by an angle alpha relative to a spectral diffraction direction of an image of the diffracted beam, where alpha is less than 10 degrees, N is at least 3, and each pixel having a height and a width defining a pixel aspect ratio greater than 2.

This invention discloses a hardware-software co-processing method for real-time spectral signal processing in Raman microscopy, belonging to the field of spectral signal processing technology. The method includes: establishing strict temporal constraints between physical displacement and cloud inference, and initiating asynchronous communication using the physical gaps in stage movement; simultaneously, deploying a deep learning model (F2P) pre-trained with a self-supervised masking strategy in the cloud. This pre-trained model utilizes the structural correlation between the Raman broadband background and narrowband characteristic peaks to directly perform real-time forward inference on the input single-frame noisy spectrum, achieving millisecond-level high-fidelity reconstruction of a single frame without any complex parameter tuning or the need for a clean control spectrum. This invention completely eliminates the physical lag in multi-frame processing and the general technical bias of supervised learning algorithms, achieving true "synchronization between acquisition and display," and significantly improving the real-time processing performance and cross-device automated deployment capabilities of in-situ Raman imaging.

The invention discloses a miniature Raman microscope with high spatial-temporal resolution. The miniature Raman microscope comprises a light source part, a micro-electro-mechanical scanning subsystem, a high-speed signal acquisition and storage subsystem and a drive control and data processing subsystem. The light source part adopts a double-optical-comb pulse light source; the micro-electro-mechanical scanning subsystem comprises a multimode optical fiber, a micro-electro-mechanical system and a gradient refractive index lens; the high-speed signal acquisition and storage subsystem comprises a short-pass filter LFP, a high-speed photoelectric detector APD and a high-speed data acquisition card; and the driving control and data processing subsystem comprises a driving control module and a data processing module and is used for controlling the light source part, the micro-electro-mechanical scanning subsystem and the high-speed signal acquisition and storage subsystem and processing the obtained data. The invention further provides a building method and application of the miniature high-temporal-spatial-resolution Raman microscope.

A method for determining if a tissue is ganglionic is provided. The method comprises a) generating at least one hyperspectral Raman image of a tissue from a region of interest in a tissue suspected to contain ganglion cells that was optionally identified by a method comprising autofluorescence (AF) and Second Harmonic generation (SHG) imaging of the tissue; and b) analyzing any of the images for one or more of the following: i) optical excitation; ii) chemical information or emission spectra; or iii) AF, SHG, and / or Raman signatures, wherein the analysis provides indicators that the region of interest is either ganglionic or non-ganglionic. A system for analysis of in vivo tissue or ex vivo tissue samples including a multiphoton autofluorescence microscope, a Second Harmonic Generation microscope, and a hyperspectral Raman microscope in operative communication is also provided.

The invention provides a coherent anti-Stokes Raman microscope system, a driving method thereof and a microscopic device. The coherent anti-Stokes Raman microscope system comprises a femtosecond laser front-end module, a dual-wavelength module, a pulse broadening amplification module, a time delay module, a spectrum broadening filtering module, a double frequency generation module, a dispersion compensation module and a microscope module. According to the coherent anti-Stokes Raman microscope system driven by self-phase modulation spectrum selection, a self-phase modulation spectrum selection (SESS) method is adopted to convert the pulse wavelength of 1550nm to 1600nm, a second harmonic generation process is realized through a frequency doubling crystal, the pulse wavelength of 1600nm is converted to 800nm, coherent anti-Stokes Raman microscopic imaging is realized, and the coherent anti-Stokes Raman microscope system driven by self-phase modulation spectrum selection has the advantages that the coherent anti-Stokes Raman microscope system is simple in structure and convenient to operate. Therefore, selective imaging of various biological tissue components is realized. Compared with other wavelength conversion methods, the device is simple, the conversion efficiency is high, the wavelength is tunable, and the expansibility is high.

The invention discloses a microscope cross-scale multi-modal correlation analysis method and a microscope cross-scale multi-modal correlation analysis system, and belongs to the technical field of microscopic imaging. According to the method, one, two or three micron-scale positioning holes are prepared or pasted on a sample carrier to serve as reference marks; the coordinates of the positioning holes are obtained under any two kinds of microscopic imaging or analysis equipment including a scanning electron microscope, an optical microscope and a Raman microscope, a coordinate conversion model between different microscopic systems is established, and accurate positioning and correlation analysis of the same sample area under the different microscopic systems are achieved; the system for realizing the method comprises a sample carrier, a positioning hole preparation module, a coordinate acquisition module and a coordinate conversion module. According to the method, the technical problem that in the prior art, accurate correlation analysis of the same microcell is difficult to carry out among different microtechnologies is solved, accurate correspondence of cross-scale multi-modal microscopic analysis is achieved, the positioning accuracy is better than 10 micrometers, and the method is suitable for comprehensive analysis and research in the fields of material science, biology, botany and the like.

This invention relates to a method for detecting indophenol sulfate based on surface-enhanced Raman spectroscopy. A liquid containing the indophenol sulfate to be detected is mixed with an oxidizing reagent to specifically oxidize the indophenol sulfate to indigo, yielding a mixed solution. This mixed solution is then mixed with gold nanoparticles, and the spectra of the mixed solution are acquired using a confocal Raman microscope. The acquired Raman spectra are baseline-removed to obtain the signal intensity at the target characteristic peak. Based on standard curves for different concentrations of indophenol sulfate and the signal intensity of the sample, the concentration of indophenol sulfate in the test sample is determined. This invention provides clear detection signals, accurate resolution, and a simple operation, enabling rapid and accurate detection of indophenol sulfate concentration in samples (such as serum). It is expected to promote research on the biological mechanisms of indophenol sulfate and its large-scale clinical application.

An anode for a lithium secondary battery and a lithium secondary battery including the same are provided. The anode for a lithium secondary battery includes an anode current collector, and an anode active material layer formed on a surface of the anode current collector. A Raman R1 value represented by ID / IG and measured on a surface of the anode active material layer is greater than 0.2 and 0.5 or less. The Raman R1 value is measured from a Raman spectrum at a laser focus level of 100% using In Via Raman Microscope from Renishaw as a Raman spectrometer.

The strain measurement device 100 for measuring mechanical strains in a sample 1 comprises a sample holder device 10 arranged to accommodate the sample 1 to be investigated, an indenter device 20 comprising an indenter tip 21 and an actuator stage 22 supporting the indenter tip 21, the actuator stage 22 being adapted to apply a load axis z via the indenter tip 21 in an indentation zone 2 of the sample 1 accommodated by the sample holder device 10 when the sample holder device 10 is in a load application position. 1 an indenter device 20 arranged to apply a localized mechanical load along an imaging axis z 2 a confocal Raman microscope device 30 arranged to collect at least one Raman spectrum in an indentation zone 2 of the sample 1, and a calculation device 40 arranged to calculate at least one strain parameter based on the at least one Raman spectrum, the sample holder device 10, the indenter device 20 and the confocal Raman microscope device 30 being arranged such that the confocal Raman microscope device 30 is able to collect the at least one Raman spectrum while the sample holder device 10 is in a load application position, the indenter device 20 and the confocal Raman microscope device 30 being aligned with a load axis z of the actuator stage 22. 1 and the imaging axis z of the confocal Raman microscope device 30. 2 Furthermore, a strain measurement method for measuring the mechanical strain in the sample 1 will be described.

1. The name of the product of this design: Spectrometer (ATR8100 Raman microscope with screen). 2. Purpose of this design product: used for micro Raman spectrometer. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: Stereoscopic diagram 1.

To provide a negative electrode for a lithium secondary battery having improved capacity characteristics and stability, and a lithium secondary battery including the same.SOLUTION: A negative electrode for a lithium secondary battery and a lithium secondary battery including the same are provided. The negative electrode for the lithium secondary battery includes a negative electrode current collector and a negative electrode active material layer formed on a surface of the negative electrode current collector. A Raman R1 value defined as ID / IG measured on a surface of the negative electrode active material layer is from 0.2 to 0.5. The Raman R1 value is measured from a Raman spectrum measured at a laser focus level of 100% using an inVia Raman microscope manufactured by Renishaw as a Raman spectroscope.SELECTED DRAWING: Figure 3

The invention relates to the technical field of analytical chemical instruments and equipment, in particular to an electrochemical Raman microscope. The electrochemical Raman microscope comprises a light path assembly, a microscopic assembly, a laser assembly, a main control board assembly, a Raman spectrometer assembly and an electrochemical analysis assembly, a microreactor for accommodating a sample to be detected is arranged below the microscopic assembly, and the microreactor is used for collecting image information of the sample to be detected; the laser assembly irradiates a to-be-tested sample with laser through the light path assembly; the Raman spectrometer assembly collects a Raman signal of the sample to be detected through the light path assembly; the electrochemical analysis assembly is electrically connected with the microreactor and is used for acquiring an electrochemical signal of the sample to be detected; and the main control board assembly is electrically connected with each assembly so as to control the acquisition of the electrochemical signal and the Raman signal at the same moment. According to the electrochemical Raman microscope provided by the invention, the Raman spectrometer, the electrochemical workstation and the microscopic light path are highly integrated, the synchronous time delay is effectively eliminated, and the real-time performance is improved, so that higher experimental requirements are met.

The invention discloses a method for quantitatively characterizing donor and acceptor distribution of an active layer of an organic photovoltaic cell, and the method comprises the steps: scanning the active layer of the organic photovoltaic cell through a Raman microscope, extracting a gray intensity value, and obtaining a Raman Mapping gray-scale map; defining a threshold value for the Raman Mapping grey-scale map, comparing the grey-scale intensity value with the threshold value, distinguishing the comparison result into different colors which respectively represent a region in which a donor material is dominated, a region in which an acceptor material is dominated and a region in which the donor and the acceptor are blended, and synthesizing a color image; and converting the regions with different colors into numerical information, and counting the number of pixel points in different regions, thereby realizing quantitative characterization of donor and acceptor distribution of the active layer of the organic photovoltaic cell. According to the method, quantitative analysis on the distribution of the donor and the acceptor materials in the organic photovoltaic cell is realized, the interaction of the donor and the acceptor materials in the active layer is conveniently and deeply understood, and a scientific basis is provided for improving the performance of the organic photovoltaic cell.

A sampling device comprising a body defining a sample receiving space, a sample collection port in communication with the sample receiving space, a sample support surface positioned within the sample receiving space, and an optical alignment and detection system, wherein the sample collection port is configured to receive a sample from a flow of material and enable delivery of the sample to the sample support surface within the sample receiving space, and a confocal Raman microscope is configured to measure properties of the sample when the sample is supported on the sample support surface.

The invention relates to a method for measuring the gas-liquid ratio of a fluid inclusion, which comprises the following steps: sample preparation: cutting a rock sample into temperature measuring sheets, polishing, and positioning a target fluid inclusion; sound tweezers are locked, wherein a standing wave sound field is generated by combining high-frequency sound waves with a piezoelectric transducer array and a holographic acoustic lens or phased array technology, and bubbles in the inclusion are locked; raman spectrum verification: before and after the acoustic tweezers are locked, scanning the inclusion by adopting a Raman spectrum technology, and verifying that the sound field operation does not cause phase change or component loss by comparing peak position offset; performing Raman three-dimensional CT scanning and deformation correction: performing layer-by-layer scanning on the inclusion by utilizing a Raman microscope, constructing three-dimensional component distribution information of the inclusion, analyzing and simulating the influence of sound radiation pressure on the bubble form through a finite element, establishing a relation model of bubble deformation and sound field intensity, and correcting an initial volume calculation result; and gas-liquid ratio calculation: calculating a gas phase volume, a liquid phase volume and a sub-mineral volume in combination with the corrected three-dimensional model.

A battery cell, a battery device, and an electric device. The battery cell comprises a positive electrode sheet, a negative electrode sheet, and an electrolyte; the lithium ion conductivity of the electrolyte is greater than or equal to 10 mS / cm; the negative electrode sheet comprises a negative electrode current collector and a negative electrode film layer disposed on at least one side of the negative electrode current collector; the negative electrode film layer comprises a negative electrode active material; the negative electrode active material comprises a core portion and a coating layer at least partially coating the surface of the core portion; the core portion comprises graphite; and in an R value cumulative distribution curve of the negative electrode active material obtained in a surface scanning mode of a laser confocal Raman microscope / spectrometer, the concentration (R90-R10) / R50 of R values is less than or equal to 2.3.

The present disclosure reveals the method for analysis of microplastics as a contaminant in food products based on nitric acid (HNO3)-based acidic, oxidative digestion of food samples at 65-69% w / w HNO3 in ultrasonic bath at 20 kHz, followed by dilution with demineralized water to the final concentration of 8-10 mol / L HNO3 and subsequent digestion at 100-110 °C during 30-100 minutes under microwave (MW) irradiation. The said method provides fast, chemoselective, and effective degradation of the food matrix, preserving the contained microplastic particles. The latter is analyzed by Raman microscopy (RM) which results in the qualitative identification of present microplastics as well as their quantitative composition and dimensions within the tested food sample. The method according to the present disclosure is used for qualitative and quantitative determination of microplastic particles as a contaminant in food products.

A sampling device comprising: a body defining a sample receiving space; a sample collection port in communication with the sample receiving space; a sample support surface positioned within the sample receiving space; and an optical alignment and detection system, wherein the sample collection port is configured to receive a sample from a stream of material and permit delivery of the sample to the sample support surface within the sample receiving space, and wherein the confocal Raman microscope is configured to measure properties of the sample when the sample is supported on the sample support surface.

The invention discloses a device and a method for collaborative analysis of a microstructure and a chemical structure. The device comprises an AFM system, a hollow motion extension mechanism, an XY plane double-axis electric displacement table and an inverted fluorescence Raman microscope, the hollowed-out movement extending mechanism is provided with a hollowed-out part used for containing a sample, and the hollowed-out movement extending mechanism is connected with the table top of the XY plane double-axis electric displacement table; the AFM system is arranged above the hollowed-out movement extension mechanism and is used for measuring the surface topography structure information and the friction performance of the target sample; and the inverted fluorescent Raman microscope is arranged below the hollow movement extension mechanism and is used for representing the chemical components of the regional sample. According to the integrated AFM and inverted fluorescent Raman microscope provided by the invention, local information of chemical components, forms, phases and molecular vibration of the material can be in one-to-one correspondence with the microscopic physical structure and size distribution of the material, and information unification of the microscopic physical structure morphology and the internal chemical component structure of the same area of the tested material is realized.

The presented embodiments relate to an advanced imaging method that integrates light-sheet Raman microscopy with photon-sparse imaging technology. This combination allows for the effective identification of targets in low-light conditions, even when light levels are below one photon per pixel. The method utilizes an accelerating Airy-beam light-sheet, which significantly expands the imaging area—by more than tenfold-compared to traditional approaches. This enhancement, in combination with single, or sparse photon detection facilitates Raman imaging at speeds comparable to video rates. Additionally, the technique incorporates photon super-localization, which boosts magnification capabilities without compromising the field of view or image resolution. An exemplary model of this integrated imaging system has been successfully applied to live-cell imaging, revealing improved photostability and providing insights into the impact of cellular noise on metabolic processes.

An anode for a lithium secondary battery and a lithium secondary battery including the same are provided. The anode for a lithium secondary battery includes an anode current collector, and an anode active material layer formed on a surface of the anode current collector. A Raman R1 value represented by ID / IG and measured on a surface of the anode active material layer is greater than about 0.2 and about 0.5 or less. The Raman R1 value is measured from a Raman spectrum at a laser focus level of 100% using InVia Raman Microscope from Renishaw as a Raman spectrometer.