26 results about "Raman microscope" patented technology

A strain measurement apparatus (100) for measuring mechanical strain in a sample (1) comprises a sample holder device (10) being arranged for accommodating the sample (1) to be investigated, an indenter device (20) including an indenter tip (21) and an actuator stage (22) carrying the indenter tip (21), wherein the actuator stage (22) is arranged for an application of a localized mechanical load along a load axis z1 via the indenter tip (21) at an indentation zone (2) of the sample (1) accommodated by the sample holder device (10), when the sample holder device (10) is in a load application position, a confocal Raman microscopy device (30) having an imaging axis z2 and being arranged for collecting at least one Raman spectrum in the indentation zone (2) of the sample (1), and a calculation device (40) being arranged for calculating at least one strain parameter based on the at least one Raman spectrum, wherein the sample holder device (10), the indenter device (20) and the confocal Raman microscopy device (30) are arranged such that the confocal Raman microscopy device (30) is capable of collecting the at least one Raman spectrum, while the sample holder device (10) is in the load application position, and the indenter device (20) and the confocal Raman microscopy device (30) are arranged such that the load axis z1 of the actuator stage (22) and the imaging axis z2 of the confocal Raman microscopy device (30) coincide. Furthermore, a strain measurement method for measuring mechanical strain in a sample (1) is described.

The invention belongs to the technical field of optical microscopic imaging, and particularly relates to a miniature coherent Raman microscope and an imaging system. The coherent Raman microscope comprises a coherent Raman excitation light path and a coherent Raman signal collection light path, the coherent Raman excitation light path sequentially comprises an excitation optical fiber, an excitation optical fiber collimating lens, a micro-electro-mechanical scanning galvanometer, a scanning lens group, a dichroscope and a finite far conjugate objective lens; the coherent Raman signal collection light path sequentially comprises a finite far conjugate objective lens, a dichroscope, a collection optical fiber collimating lens and a collection optical fiber. According to the invention, a micro-electro-mechanical galvanometer and a voice coil motor are used for scanning a sample; according to the light path design, the light path structure is greatly simplified, the collection efficiency of the signal light is improved, and good imaging quality is ensured. The miniature coherent Raman microscopic imaging system overcomes the problem that unmarked in-situ imaging cannot be achieved due to the fact that a traditional coherent Raman microscope is too large in size, meanwhile, the imaging quality equivalent to that of the traditional coherent Raman microscope is kept, and the miniature coherent Raman microscopic imaging system has wide application prospects.

The invention discloses a spiral chiral gold nanorod as well as a preparation method and application thereof, and the method comprises the following steps: (1) sequentially adding hexadecyl trimethyl ammonium bromide, cysteine and p-aminothiophenol into an anisotropic gold nanorod solution, uniformly mixing, and adsorbing; and (2) silver nitrate, chloroauric acid and ascorbic acid are added for a reaction, centrifugal dispersion is performed after the reaction is finished, and the spiral chiral gold nanorod is obtained. Dispersing the spiral chiral gold nanorod in an aqueous solution, adding phenylalanine enantiomer, dropwise adding on a clean glass slide after incubation is finished, and performing surface enhanced Raman spectroscopy test by using a Raman microscope after air drying. According to the invention, the broadband coverage of chiral response from visible light to near ultraviolet region is realized by accurately regulating and controlling the arrangement of interface molecules and the morphology of the nano structure.

The invention provides a method for in-situ detection of damp-heat aging of an underfill and a detection system. The method comprises the following steps: (1) providing an underfill sample; (2) placing the bottom filling glue sample on a temperature and humidity control sample table, wherein the temperature and humidity control sample table is combined with a confocal microscopic Raman spectrometer; (3) adjusting the parameters of the temperature and humidity control sample table, and adjusting the parameters of the confocal microscopic Raman spectrometer; (4) performing in-situ Raman spectrum characterization on a certain site of the initial bottom filling glue sample under the conditions set in the step (3), then performing damp-heat aging on the bottom filling glue sample, and performing in-situ Raman spectrum characterization on the same test site of the bottom filling glue sample at regular intervals, and analyzing the change of the characteristic peak according to the obtained Raman spectrum information so as to determine the damp-heat aging mechanism of the underfill sample. The invention provides a method for in-situ detection of real-time chemical bond change information of underfill in a damp-heat aging process.

An automated portable Raman microscope combines the simplicity, ruggedness, and selectivity of Raman spectroscopy with the sensitivity of single-particle identification. It includes a visible camera, controllable illumination, and a moving optical head that together acquire images of the sample at different focal planes and under different lighting conditions. An internal processor automatically identifies particles or other targets in these images for Raman analysis, e.g., shifted excitation difference Raman spectroscopy. The moving optical head scans the Raman pump beam across the sample to different targets. A built-in user interface displays the results of the Raman analysis superimposed on visual images of the sample. The Raman microscope's simplicity, reliability, and low maintenance make it easy to use for a wide variety of applications, including analyzing and identifying particles and residues, linking chemical and biometric evidence, and forensics analysis.

The invention provides a method and a device for measuring the transverse spatial resolution of a confocal Raman microscope, and relates to the technical field of optical measurement and micro-spectrum. The method comprises the following steps: positioning a linear edge area on a single-layer graphene sample by using a confocal Raman microscope, and preparing the single-layer graphene sample on a silicon oxide substrate; determining a linear scanning path and a scanning step length from the exposed area of the substrate to the sample area in the positioned area of the linear edge along the direction vertical to the linear edge by using a confocal Raman microscope; in the moving process of the single-layer graphene sample, determining a plurality of scanning points on the linear scanning path based on the scanning step length, and collecting a Raman spectrum signal of each scanning point; and determining the transverse spatial resolution of the confocal Raman microscope based on the Raman spectrum of each scanning point. By adopting the method provided by the invention, the accuracy of the transverse spatial resolution of the confocal Raman microscope can be improved.

The application provides a method and device for measuring lateral spatial resolution of a confocal Raman microscope, and relates to the field of optical measurement and microscopic spectroscopy technology. The method comprises the following steps: positioning a region of a straight edge on a single-layer graphene sample by using a confocal Raman microscope, wherein the single-layer graphene sample is prepared on a silicon oxide substrate; determining a straight scanning path and a scanning step in a direction perpendicular to the straight edge from a bare region of the substrate to a sample region in the region of the straight edge which has been positioned by using the confocal Raman microscope; determining a plurality of scanning points on the straight scanning path based on the scanning step in the process of moving the single-layer graphene sample, and collecting Raman spectrum signals of the scanning points; and measuring the lateral spatial resolution of the confocal Raman microscope based on the Raman spectrum of each scanning point. By using the method provided in the application, the accuracy of the lateral spatial resolution of the confocal Raman microscope can be improved.

The invention relates to a spectrometer (10) comprising an aperture (13) configured to receive a source light beam, a first optical system configured to receive the source light beam and form a collimated light beam directed to a diffraction grating, the diffraction grating configured to receive the collimated light beam and form a diffracted light beam, a second optical system configured to form an image of the diffracted light beam on an image sensor. According to the invention, the image sensor is a CMOS sensor comprising pixels (34) arranged in N rows, the pixel rows being oriented in a direction inclined by an angle alpha relative to a spectral diffraction direction of an image of the diffracted beam, where alpha is less than 10 degrees, N is at least 3, and each pixel having a height and a width defining a pixel aspect ratio greater than 2.

This invention discloses a hardware-software co-processing method for real-time spectral signal processing in Raman microscopy, belonging to the field of spectral signal processing technology. The method includes: establishing strict temporal constraints between physical displacement and cloud inference, and initiating asynchronous communication using the physical gaps in stage movement; simultaneously, deploying a deep learning model (F2P) pre-trained with a self-supervised masking strategy in the cloud. This pre-trained model utilizes the structural correlation between the Raman broadband background and narrowband characteristic peaks to directly perform real-time forward inference on the input single-frame noisy spectrum, achieving millisecond-level high-fidelity reconstruction of a single frame without any complex parameter tuning or the need for a clean control spectrum. This invention completely eliminates the physical lag in multi-frame processing and the general technical bias of supervised learning algorithms, achieving true "synchronization between acquisition and display," and significantly improving the real-time processing performance and cross-device automated deployment capabilities of in-situ Raman imaging.

The invention discloses a microscope cross-scale multi-modal correlation analysis method and a microscope cross-scale multi-modal correlation analysis system, and belongs to the technical field of microscopic imaging. According to the method, one, two or three micron-scale positioning holes are prepared or pasted on a sample carrier to serve as reference marks; the coordinates of the positioning holes are obtained under any two kinds of microscopic imaging or analysis equipment including a scanning electron microscope, an optical microscope and a Raman microscope, a coordinate conversion model between different microscopic systems is established, and accurate positioning and correlation analysis of the same sample area under the different microscopic systems are achieved; the system for realizing the method comprises a sample carrier, a positioning hole preparation module, a coordinate acquisition module and a coordinate conversion module. According to the method, the technical problem that in the prior art, accurate correlation analysis of the same microcell is difficult to carry out among different microtechnologies is solved, accurate correspondence of cross-scale multi-modal microscopic analysis is achieved, the positioning accuracy is better than 10 micrometers, and the method is suitable for comprehensive analysis and research in the fields of material science, biology, botany and the like.

This invention relates to a method for detecting indophenol sulfate based on surface-enhanced Raman spectroscopy. A liquid containing the indophenol sulfate to be detected is mixed with an oxidizing reagent to specifically oxidize the indophenol sulfate to indigo, yielding a mixed solution. This mixed solution is then mixed with gold nanoparticles, and the spectra of the mixed solution are acquired using a confocal Raman microscope. The acquired Raman spectra are baseline-removed to obtain the signal intensity at the target characteristic peak. Based on standard curves for different concentrations of indophenol sulfate and the signal intensity of the sample, the concentration of indophenol sulfate in the test sample is determined. This invention provides clear detection signals, accurate resolution, and a simple operation, enabling rapid and accurate detection of indophenol sulfate concentration in samples (such as serum). It is expected to promote research on the biological mechanisms of indophenol sulfate and its large-scale clinical application.

The invention discloses a method for quantitatively characterizing donor and acceptor distribution of an active layer of an organic photovoltaic cell, and the method comprises the steps: scanning the active layer of the organic photovoltaic cell through a Raman microscope, extracting a gray intensity value, and obtaining a Raman Mapping gray-scale map; defining a threshold value for the Raman Mapping grey-scale map, comparing the grey-scale intensity value with the threshold value, distinguishing the comparison result into different colors which respectively represent a region in which a donor material is dominated, a region in which an acceptor material is dominated and a region in which the donor and the acceptor are blended, and synthesizing a color image; and converting the regions with different colors into numerical information, and counting the number of pixel points in different regions, thereby realizing quantitative characterization of donor and acceptor distribution of the active layer of the organic photovoltaic cell. According to the method, quantitative analysis on the distribution of the donor and the acceptor materials in the organic photovoltaic cell is realized, the interaction of the donor and the acceptor materials in the active layer is conveniently and deeply understood, and a scientific basis is provided for improving the performance of the organic photovoltaic cell.

A sampling device comprising a body defining a sample receiving space, a sample collection port in communication with the sample receiving space, a sample support surface positioned within the sample receiving space, and an optical alignment and detection system, wherein the sample collection port is configured to receive a sample from a flow of material and enable delivery of the sample to the sample support surface within the sample receiving space, and a confocal Raman microscope is configured to measure properties of the sample when the sample is supported on the sample support surface.

The invention relates to a method for measuring the gas-liquid ratio of a fluid inclusion, which comprises the following steps: sample preparation: cutting a rock sample into temperature measuring sheets, polishing, and positioning a target fluid inclusion; sound tweezers are locked, wherein a standing wave sound field is generated by combining high-frequency sound waves with a piezoelectric transducer array and a holographic acoustic lens or phased array technology, and bubbles in the inclusion are locked; raman spectrum verification: before and after the acoustic tweezers are locked, scanning the inclusion by adopting a Raman spectrum technology, and verifying that the sound field operation does not cause phase change or component loss by comparing peak position offset; performing Raman three-dimensional CT scanning and deformation correction: performing layer-by-layer scanning on the inclusion by utilizing a Raman microscope, constructing three-dimensional component distribution information of the inclusion, analyzing and simulating the influence of sound radiation pressure on the bubble form through a finite element, establishing a relation model of bubble deformation and sound field intensity, and correcting an initial volume calculation result; and gas-liquid ratio calculation: calculating a gas phase volume, a liquid phase volume and a sub-mineral volume in combination with the corrected three-dimensional model.

A battery cell, a battery device, and an electric device. The battery cell comprises a positive electrode sheet, a negative electrode sheet, and an electrolyte; the lithium ion conductivity of the electrolyte is greater than or equal to 10 mS / cm; the negative electrode sheet comprises a negative electrode current collector and a negative electrode film layer disposed on at least one side of the negative electrode current collector; the negative electrode film layer comprises a negative electrode active material; the negative electrode active material comprises a core portion and a coating layer at least partially coating the surface of the core portion; the core portion comprises graphite; and in an R value cumulative distribution curve of the negative electrode active material obtained in a surface scanning mode of a laser confocal Raman microscope / spectrometer, the concentration (R90-R10) / R50 of R values is less than or equal to 2.3.

A sampling device comprising: a body defining a sample receiving space; a sample collection port in communication with the sample receiving space; a sample support surface positioned within the sample receiving space; and an optical alignment and detection system, wherein the sample collection port is configured to receive a sample from a stream of material and permit delivery of the sample to the sample support surface within the sample receiving space, and wherein the confocal Raman microscope is configured to measure properties of the sample when the sample is supported on the sample support surface.

The presented embodiments relate to an advanced imaging method that integrates light-sheet Raman microscopy with photon-sparse imaging technology. This combination allows for the effective identification of targets in low-light conditions, even when light levels are below one photon per pixel. The method utilizes an accelerating Airy-beam light-sheet, which significantly expands the imaging area—by more than tenfold-compared to traditional approaches. This enhancement, in combination with single, or sparse photon detection facilitates Raman imaging at speeds comparable to video rates. Additionally, the technique incorporates photon super-localization, which boosts magnification capabilities without compromising the field of view or image resolution. An exemplary model of this integrated imaging system has been successfully applied to live-cell imaging, revealing improved photostability and providing insights into the impact of cellular noise on metabolic processes.

To deal with the situation where conducting a Raman microscope observation with an optical microscope requires the optical microscope to have a plurality of filter turrets and it is not therefore easy to conduct a Raman microscope observation with an ordinary fluorescent microscope and a special microscope is needed.SOLUTION: There is provided a spectral device with an image observation function, connectable to at least one camera port generally equipped to an optical microscope and being capable of conducting a spectral measurement of Raman scattered light.SELECTED DRAWING: Figure 7

This invention specifically relates to a method for determining the uniformity of acetylation reaction in modified starch granules. It employs the characteristic peak "intensity ratio method," using a confocal Raman microscope to acquire the Raman spectrum of individual starch granules. The Raman intensity ratio of the characteristic vibrational peak of the ester group (-C=O) to the internal standard peak of the starch skeleton represents the relative magnitude of the modification reaction in a single acetylated modified starch granule. Based on a large number of Raman intensity ratios, a frequency distribution histogram is plotted, and statistical parameters are used to quantify the uniformity of the reaction. Methodological validation results show that this method has good accuracy. This technology solves the technical problem that traditional methods cannot assess the uniformity of acetylation reaction between granules, providing a reliable means for quality control and process optimization of high-quality acetylated starch.

The present invention relates to an optical spectrometer (10) comprising: an aperture (13) configured to receive a light source beam; a first optical system configured to receive the light source beam and form a collimated light beam directed toward a diffraction grating; a diffraction grating configured to receive the collimated light beam and form a diffracted light beam; and a second optical system configured to form an image of the diffracted light beam on an image sensor. According to the present invention, the image sensor is a CMOS sensor comprising pixels (34) arranged in N rows oriented in a direction tilted by an angle alpha with respect to the spectral diffraction direction of the image of the diffracted light beam, where alpha is less than 10 degrees, N is at least 3, and each pixel has a height and width that define a pixel aspect ratio greater than 2.

This application discloses a negative electrode active material and a method for manufacturing the same, a negative electrode sheet, a lithium-ion battery, and a power consumption device. The lithium-ion battery includes one or more battery cells including a negative electrode sheet. The negative electrode sheet includes a negative electrode current collector and a negative electrode film layer located on at least one surface of the negative electrode current collector and including a negative electrode active material. The negative electrode active material includes a core including graphite and a coating layer located on at least a portion of the surface of the core and including a hard carbon. The negative electrode active material has an R50 of 0.15 to 0.40, which is the R value at which 50% of the cumulative distribution from the lower limit is obtained using a laser confocal Raman microscope in area scanning mode. The negative electrode active material provided herein enables batteries to combine high energy density with good dynamic and cycle characteristics.

This invention provides a Skin-Resolved Diffusion (SERS) Detection System for Deep-Resolved Drug Diffusion, comprising a SERS-active microneedle array and a confocal Raman microscope. The SERS-active microneedle array is used for in-situ drug detection at multiple depths in the skin. It consists of polymer microneedles of varying lengths, with noble metal core-shell nanostars uniformly immobilized on their surfaces. Each noble metal core-shell nanostar comprises a noble metal nanostar core modified with an internal standard reporter molecule and an outer noble metal shell. The confocal Raman microscope records the SERS signal generated after the microneedles penetrate the skin. A quantitative model is established by comparing the peak ratio of the target drug characteristic peak to the internal standard characteristic peak in the SERS signal to determine the drug concentration at different depths. This invention enables quantitative analysis of the diffusion behavior of topical drugs at different skin types and depths.

1. The name of the product under this design: Confocal Raman Microscope (CVRam HRS300). 2. Purpose of this design product: used for the outer shell of a microscopic confocal Raman instrument. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

Provided herein are methods of using a machine learning model, e.g., optimal transport, in analyzing responses of biological samples to drugs and drug combinations, as determined by infrared and / or Raman microscopy.

The invention belongs to the technical field of cell culture, and particularly relates to a Raman spectrum-based culture medium optimization method and a cell culture medium, the method comprises the following steps: (1) adding different amounts of effective components into a basic culture medium to prepare a plurality of cell culture mediums, the basic culture medium does not contain the effective components; (2) respectively culturing the cells by adopting the plurality of cell culture mediums to obtain different culture solutions containing the cells; and (3) respectively placing the different culture solutions containing the cells under a Raman microscope, and collecting Raman spectrums of the cells in the different culture solutions so as to obtain the optimized cell culture medium. According to the culture medium optimization method provided by the invention, the Raman spectrum is adopted for detecting cells in the culture medium for the first time so as to optimize the dosage of effective components in the cells, and the optimized culture medium can meet the requirements of the effective components for cell growth and cannot cause waste of the effective components.

To provide optical configurations for confocal structured illumination Raman (C-SIM Raman) microscopy systems.SOLUTION: One example includes: a light source configured to project a light; a first set of optical components fixedly aligned along a first optical path, where the first set of optical components includes a vortex phase plate and a quarter wave plate, where the first set of optical components is configured to receive the light; and a second set of optical components configured to be adjusted between a first operating position and a second operating position. When in the first operating position, the second set of optical components is aligned on the first optical path and is configured to expand, in conjunction with the first set of optical components, the light into an expanded light. When in the second operating position, the second set of optical components is not aligned on the first optical path.SELECTED DRAWING: Figure 3