18 results about "Chemical imaging" patented technology

The invention relates to the field of electrochemiluminescence imaging analysis, and particularly discloses a single-particle electrochemiluminescence imaging method by adjusting the thickness of a molecular self-assembly layer, which is used for solving the problems of serious non-specific luminescence of an electrode substrate, incapability of finely regulating an interface structure, poor stability and the like in single-particle electrochemiluminescence imaging. The method comprises the following steps: preparing an insulating molecule self-assembly layer with gradient thickness, optimizing a fixing process of gold nanoparticles, determining the position of a single particle, carrying out ECL imaging acquisition on the single particle, and optimizing the thickness of the molecule self-assembly layer. According to the method, the thickness of a molecular self-assembly layer is precisely regulated and controlled, a nano-particle fixing and ECL system is optimized, and a single-particle ECL imaging device is built, so that the signal-to-noise ratio of single-particle electrochemical imaging can be increased, and background interference caused by non-specific luminescence of an electrode substrate is effectively inhibited.

The invention discloses a CARS-LA-ICP-MS combined multimode analysis system. The CARS-LA-ICP-MS combined multimode analysis system comprises a fusion light path module, a mass spectrometry gas path module, a three-dimensional mobile station, a CARS signal acquisition system and a computer control system, the fusion light path module comprises a laser source, an optical parameter amplifier, a light pulse time delay system, three dichroscopes, a light beam adjusting system, a galvanometer, a movable objective lens and a camera; the mass spectrometry gas circuit module comprises a carrier gas supply device, a multimode analysis sample cell and a mass spectrometry device; the multimode analysis sample cell comprises an upper cover, a lower cover and a side wall shell. On the other hand, the invention discloses an analysis method which comprises initialization setting, CARS analysis, mass spectrum analysis task parameter setting, laser ablation mass spectrum analysis and data synthesis. Through coaxial optical path design and an intelligent algorithm, deep fusion of chemical imaging and elemental analysis is realized, and a brand new research tool is provided for the fields of life science, material science and the like.

The invention relates to a microplastic visualization method and device, electronic equipment, a storage medium and a program product. The method comprises the following steps: obtaining a Raman spectrum of a micro-plastic sample; based on the Raman spectrum, determining the peak top and the peak bottom of the characteristic peak of the micro-plastic in the micro-plastic sample; and based on difference information between the peak top and the peak bottom of the characteristic peak of the micro-plastic, performing chemical imaging extraction on the Raman spectrum to generate a chemical imaging image of the micro-plastic sample.

The invention discloses an in-situ pulp incubation method for click chemical imaging analysis of postharvest lignification of fruits. The in-situ pulp incubation method comprises the following steps: (1) preparing a high-concentration hydrogel solution and a low-concentration hydrogel solution; (2) preparing a CA-Alk working solution and a CA-Alk-containing hydrogel incubation solution; (3) cutting a wound on the fruit with a knife to expose pulp, soaking the wound of the fruit in a CA-Alk working solution for 24 hours, taking out the fruit, then coating the surface of the wound with a hydrogel incubation solution containing CA-Alk, and curing to form a first gel layer; and then coating the first gel layer with the high-concentration hydrogel liquid, and coating the first gel layer with a preservative film for fixation. According to the method, the integrity of pulp cells is almost not damaged when observed under an optical microscope, and the incubation time can last from original 1-2 days to 7 days or above. Meanwhile, due to in-situ incubation, the reaction on the lignin accumulation rule is more accurate.

An in-situ chemical imaging method and system applied to a soil drilosphere. The method comprises: S1, preparing an earthworm burrowing box and filling same with soil; S2, selecting appropriate earthworms and placing same in the soil for culturing, such that sufficient earthworm pores are generated in the soil; S3, sequentially providing a filter membrane (9), a DGT adsorption membrane (107) and a pH planar optode membrane (106) on a soil profile of a drilosphere which needs to be imaged, such that the three membranes can all be fully attached to the soil profile of the drilosphere, applying ultraviolet light under dark conditions, culturing for a period of time, and capturing a fluorescence signal emitted by the pH planar optode membrane (106), so as to obtain a pH fluorescence image; S4, performing color development on the DGT adsorption membrane (107); and S5, capturing a color-developed image of the DGT adsorption membrane (107) to obtain a DGT image, and performing picture processing on the DGT image and the pH fluorescence image to obtain spatial distribution images of available phosphorus and pH in the drilosphere. The problem of difficulty in in-situ detection of a biogeochemical cycling process driven by earthworm activities in soil is solved.

Systems and methods for sensing vibrational absorption induced photothermal effect via a visible light source. A Mid-infrared photothermal probe (MI-PTP, or MIP) approach achieves 10 mM detection sensitivity and sub-micron lateral spatial resolution. Such performance exceeds the diffraction limit of infrared microscopy and allows label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells can be visualized. MIP imaging technology may enable applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

To provide a chemical imaging device that can reduce the effort required of users when measuring the shape of chemicals, as well as a chemical shape measuring device and a chemical dispensing device equipped with the same. [Solution] The drug imaging device 7 comprises a drug holding unit 81, an illumination unit 82, an imaging unit 83, a housing 71, and a rotating support unit. The drug holding unit 81, the illumination unit 82, and the imaging unit 83 are housed in a light-shielding housing. The drug holding unit 81 is positioned between the illumination unit 82 and the imaging unit 83, has a light-transmitting portion that can hold drugs, and is rotatable by the rotating support unit.

A method for analysing a specimen in a microscope is provided. The method comprises: acquiring a series of compound image frames using an electron detector, a first X-ray detector, and a second X-ray detector, wherein the first X-ray detector is positioned between the specimen and an electron beam source from which a focused electron beam emerges towards the specimen, and is provided with a filter member interposed between the first X-ray detector and the specimen and adapted to reduce the incidence of electrons on the first X-ray detector, wherein the second X-ray detector is provided with a deflector arrangement configured to reduce the incidence of electrons on the second X-ray detector, and wherein acquiring a compound image frame comprises: causing the focused electron beam to traverse a region of the specimen; monitoring a set of resulting electrons emitted from a plurality of locations within the region of the specimen, using the electron detector, so as to obtain a first image frame, the first image frame comprising a plurality of pixels corresponding to, and having values derived from the monitored electrons emitted from, the plurality of locations; monitoring first and second sets of resulting X-rays emitted from the plurality of locations using the first X-ray detector and the second X-ray detector respectively, so as to obtain one or more second image frames, each comprising a plurality of pixels corresponding to the plurality of locations and having values derived, in accordance with a first criterion, from monitored X rays characteristic of a respective chemical element and emitted from the plurality of locations, wherein the set of electrons and the first and second sets of X-rays are emitted from the specimen substantially simultaneously; and combining the first image frame and the one or more second image frames so as to produce the compound image frame, such that the compound image frame provides data derived from monitored electrons and X-rays emitted from the plurality of locations within the region, and displaying the series of compound image frames on a visual display, wherein the visual display is updated to show each compound image frame in sequence. A system for analysing a specimen in a microscope is also provided.

The invention belongs to the field of micro-plastic pollutant detection, and particularly relates to a method for identifying micro-plastic in blood based on a laser direct infrared imaging system, and the method mainly comprises the following steps: digesting the blood by using 68% concentrated nitric acid; filtering the digested mixed solution through a filter membrane; rinsing the filter membrane; micro plastic particles are enriched; concentrating and transferring the micro plastic particles; dropwise adding a micro-plastic sample on the glass window and drying; a laser direct infrared chemical imaging system is used for identifying the micro-plastic particles in the blood and the apparent characteristics of the micro-plastic particles. The method disclosed by the invention not only can efficiently and simply extract and collect the micro-plastics in the blood, but also can quickly and accurately identify and analyze the types and apparent characteristics of the micro-plastics, effectively makes up the defects of high detection cost, low detection efficiency and the like of a traditional detection method, has a great application potential in detection of the micro-plastics in the blood of the living body, and has a wide application prospect. The important support is provided for comprehensively analyzing the health risk of the micro-plastic to the life organism.

Embodiments disclosed include methods and apparatus for Fluorescent Enhanced Photothermal Infrared (FE-PTIR) spectroscopy and chemical imaging, which enables high sensitivity and high spatial resolution measurements of IR absorption with simultaneous confocal fluorescence imaging. In various embodiments, the FE-PTIR technique utilizes combined / simultaneous OPTIR and fluorescence imaging that provides significant improvements and benefits compared to previous work by simultaneous detection of both IR absorption and confocal fluorescence using the same optical detector at the same time.

Methods and apparatus for spectroscopy from nanoscale to millimeter scale and imaging techniques, including atomic force microscopy, infrared spectroscopy, confocal microscopy, Raman spectroscopy, and mass spectroscopy. For infrared spectroscopy, the sample is illuminated with infrared light and the resulting sample deformation is read out with a focused UV / visible beam and / or AFM tip. The combination of the two techniques provides fast and large area measurement scans using the UV / visible light and high resolution measurements using the AFM tip. The methods and apparatus also include the ability to analyze the light reflected / scattered from the sample by a Raman spectrometer for supplemental analysis by Raman spectroscopy.

The invention relates to the technical field of pharmaceutical analysis, in particular to a crystal form detection method for Pinefibrate in Pinefibrate tablets, which comprises the following steps: acquiring a sample to be detected from the Pinefibrate tablets; detecting the sample to be detected by using a chemical imaging method based on a vibration spectrum to obtain spectral data; and comparing the spectral data with the spectral data of the known crystal form of Pinefibrate, and identifying the crystal form of Pinefibrate. The method for detecting the crystal form of the pterofibrate in the pterofibrate tablet has the beneficial effects that a Raman spectrometer is used for qualitatively testing the crystal form of a pterofibrate bulk drug in a preparation, complicated pretreatment on a sample is not needed, the risk of crystal transformation possibly caused by pretreatment is avoided, and the sample loading process of the sample is simplified. Meanwhile, the sample is directly scanned and imaged, the position of the pterofibrate bulk drug is accurately positioned, the interference of auxiliary materials is reduced, and the crystal form of pterofibrate in the preparation is rapidly measured.

The invention relates to the technical field of computer vision and environmental monitoring, and discloses a microplastic component rapid identification method based on multi-modal data driving, and the method comprises the steps: firstly obtaining a morphological sequence image and a chemical component map of microplastic, and constructing a standardized three-dimensional voxel data set through metadata alignment; then constructing an uncoupled training set and executing three-dimensional data enhancement based on spatial transformation; training a component identification network by using the enhanced data, and establishing a mapping relation between the micro-plastic three-dimensional morphological characteristics and chemical components; and finally, acquiring a morphological image of a sample to be detected, inputting the morphological image into the network, and outputting a three-dimensional segmentation mask with a chemical component code. According to the method, the deep relation between forms and components is excavated, so that the chemical components and three-dimensional distribution of the micro-plastics are quickly and accurately identified only according to low-cost morphological images, the problems of high cost and low speed of traditional chemical imaging are solved, and the method is suitable for efficient monitoring of environmental micro-plastic pollution.

The invention belongs to the technical field of multi-modal combined imaging, and discloses a multi-modal combined imaging method and platform for shale nano-pore three-dimensional reconstruction and connectivity quantitative evaluation, and aims to realize shale organic pore and inorganic pore structure three-dimensional and quantitative characterization based on FIB-SEM and X-CT. High-resolution imaging and chemical imaging technologies are used for revealing the occurrence state of kerogen in mineral particles or at the boundaries, the mineral surface catalyzes organic matter to generate hydrocarbon, and the pore evolution mechanism is regulated and controlled.

The application discloses a self-powered photoelectrochemical imaging system and a preparation method thereof, and belongs to the technical field of photoelectric detection and imaging. The imaging system comprises a flexible substrate, a liquid metal electrode array formed on the surface of the flexible substrate, a photoelectric detection unit array composed of a vanadium oxide photosensitive layer formed on part of the surface of the liquid metal electrode array and a natural gallium oxide counter electrode layer formed on the rest of the surface of the liquid metal electrode array, the vanadium oxide photosensitive layer and the gallium oxide counter electrode layer are arranged alternately and form a planar heterojunction, the planar heterojunction is used for realizing self-power supply of the system, a solid electrolyte layer is filled between each unit of the photoelectric detection unit array, and an encapsulation layer is wrapped outside each functional layer. High-definition imaging in the visible light region can be realized, and the application is suitable for high-precision detection scenes such as biomedical micro-imaging and precision industrial detection.

The application discloses a CARS-LA-ICP-MS combined multi-mode analysis system, which comprises a fusion optical path module, a mass spectrum analysis gas path module, a three-dimensional moving table, a CARS signal acquisition system and a computer control system; the fusion optical path module comprises a laser source, an optical parametric amplifier, an optical pulse time delay system, three dichroic mirrors, a light beam adjusting system, a galvanometer, a moving objective and a camera; the mass spectrum analysis gas path module comprises a carrier gas supply device, a multi-mode analysis sample pool and a mass spectrum analysis device; the multi-mode analysis sample pool comprises an upper cover, a lower cover and a side wall shell. The application discloses another aspect of an analysis method, which comprises initialization setting, CARS analysis, mass spectrum analysis task parameter setting, laser ablation mass spectrum analysis and data synthesis. Through coaxial optical path design and intelligent algorithm, the application realizes deep fusion of chemical imaging and element analysis, and provides a new research tool for the fields of life science, material science and the like.

The application relates to the technical field of computer vision and environment monitoring, and discloses a microplastic component rapid identification method based on multi-modal data driving, which comprises the following steps: firstly, acquiring morphological sequence images and chemical component maps of microplastics, and constructing a standardized three-dimensional voxel dataset through metadata alignment; then, constructing a non-coupling training set and performing three-dimensional data enhancement based on spatial transformation; training a component identification network by using the enhanced data, and establishing a mapping relationship between the three-dimensional morphological characteristics of the microplastics and the chemical components; finally, collecting morphological images of samples to be measured and inputting the images into the network, and outputting three-dimensional segmentation masks with chemical component codes. The application realizes rapid and accurate identification of the chemical components and three-dimensional distribution of microplastics only according to low-cost morphological images by mining the deep connection between the morphology and the components, solves the problems of high cost and slow speed of traditional chemical imaging, and is suitable for efficient monitoring of environmental microplastic pollution.

The invention relates to the technical field of intelligent imaging, and discloses a micro-area hyperspectral chemical imaging method and system based on artificial intelligence, a terminal and a storage medium. The method comprises the following steps: acquiring a three-dimensional hyperspectral data cube of a standard sample, and generating a region-of-interest mask through unsupervised clustering; extracting space-spectrum joint feature image blocks to construct a training data set; training a three-dimensional convolutional neural network model integrating residual connection and multiple attention mechanisms; pixel-level classification prediction is carried out on the standard sample to be detected in a sliding window mode; and generating a chemical substance spatial distribution diagram. The method can realize high-precision identification of various chemical substances, and has the advantages of high throughput, low cost, nondestructive detection and the like.