32 results about "Chemical imaging" patented technology

The invention relates to the field of electrochemiluminescence imaging analysis, and particularly discloses a single-particle electrochemiluminescence imaging method by adjusting the thickness of a molecular self-assembly layer, which is used for solving the problems of serious non-specific luminescence of an electrode substrate, incapability of finely regulating an interface structure, poor stability and the like in single-particle electrochemiluminescence imaging. The method comprises the following steps: preparing an insulating molecule self-assembly layer with gradient thickness, optimizing a fixing process of gold nanoparticles, determining the position of a single particle, carrying out ECL imaging acquisition on the single particle, and optimizing the thickness of the molecule self-assembly layer. According to the method, the thickness of a molecular self-assembly layer is precisely regulated and controlled, a nano-particle fixing and ECL system is optimized, and a single-particle ECL imaging device is built, so that the signal-to-noise ratio of single-particle electrochemical imaging can be increased, and background interference caused by non-specific luminescence of an electrode substrate is effectively inhibited.

The invention discloses a CARS-LA-ICP-MS combined multimode analysis system. The CARS-LA-ICP-MS combined multimode analysis system comprises a fusion light path module, a mass spectrometry gas path module, a three-dimensional mobile station, a CARS signal acquisition system and a computer control system, the fusion light path module comprises a laser source, an optical parameter amplifier, a light pulse time delay system, three dichroscopes, a light beam adjusting system, a galvanometer, a movable objective lens and a camera; the mass spectrometry gas circuit module comprises a carrier gas supply device, a multimode analysis sample cell and a mass spectrometry device; the multimode analysis sample cell comprises an upper cover, a lower cover and a side wall shell. On the other hand, the invention discloses an analysis method which comprises initialization setting, CARS analysis, mass spectrum analysis task parameter setting, laser ablation mass spectrum analysis and data synthesis. Through coaxial optical path design and an intelligent algorithm, deep fusion of chemical imaging and elemental analysis is realized, and a brand new research tool is provided for the fields of life science, material science and the like.

The invention relates to a microplastic visualization method and device, electronic equipment, a storage medium and a program product. The method comprises the following steps: obtaining a Raman spectrum of a micro-plastic sample; based on the Raman spectrum, determining the peak top and the peak bottom of the characteristic peak of the micro-plastic in the micro-plastic sample; and based on difference information between the peak top and the peak bottom of the characteristic peak of the micro-plastic, performing chemical imaging extraction on the Raman spectrum to generate a chemical imaging image of the micro-plastic sample.

The invention discloses an in-situ pulp incubation method for click chemical imaging analysis of postharvest lignification of fruits. The in-situ pulp incubation method comprises the following steps: (1) preparing a high-concentration hydrogel solution and a low-concentration hydrogel solution; (2) preparing a CA-Alk working solution and a CA-Alk-containing hydrogel incubation solution; (3) cutting a wound on the fruit with a knife to expose pulp, soaking the wound of the fruit in a CA-Alk working solution for 24 hours, taking out the fruit, then coating the surface of the wound with a hydrogel incubation solution containing CA-Alk, and curing to form a first gel layer; and then coating the first gel layer with the high-concentration hydrogel liquid, and coating the first gel layer with a preservative film for fixation. According to the method, the integrity of pulp cells is almost not damaged when observed under an optical microscope, and the incubation time can last from original 1-2 days to 7 days or above. Meanwhile, due to in-situ incubation, the reaction on the lignin accumulation rule is more accurate.

An in-situ chemical imaging method and system applied to a soil drilosphere. The method comprises: S1, preparing an earthworm burrowing box and filling same with soil; S2, selecting appropriate earthworms and placing same in the soil for culturing, such that sufficient earthworm pores are generated in the soil; S3, sequentially providing a filter membrane (9), a DGT adsorption membrane (107) and a pH planar optode membrane (106) on a soil profile of a drilosphere which needs to be imaged, such that the three membranes can all be fully attached to the soil profile of the drilosphere, applying ultraviolet light under dark conditions, culturing for a period of time, and capturing a fluorescence signal emitted by the pH planar optode membrane (106), so as to obtain a pH fluorescence image; S4, performing color development on the DGT adsorption membrane (107); and S5, capturing a color-developed image of the DGT adsorption membrane (107) to obtain a DGT image, and performing picture processing on the DGT image and the pH fluorescence image to obtain spatial distribution images of available phosphorus and pH in the drilosphere. The problem of difficulty in in-situ detection of a biogeochemical cycling process driven by earthworm activities in soil is solved.

Systems and methods for sensing vibrational absorption induced photothermal effect via a visible light source. A Mid-infrared photothermal probe (MI-PTP, or MIP) approach achieves 10 mM detection sensitivity and sub-micron lateral spatial resolution. Such performance exceeds the diffraction limit of infrared microscopy and allows label-free three-dimensional chemical imaging of live cells and organisms. Distributions of endogenous lipid and exogenous drug inside single cells can be visualized. MIP imaging technology may enable applications from monitoring metabolic activities to high-resolution mapping of drug molecules in living systems, which are beyond the reach of current infrared microscopy.

To provide a chemical imaging device that can reduce the effort required of users when measuring the shape of chemicals, as well as a chemical shape measuring device and a chemical dispensing device equipped with the same. [Solution] The drug imaging device 7 comprises a drug holding unit 81, an illumination unit 82, an imaging unit 83, a housing 71, and a rotating support unit. The drug holding unit 81, the illumination unit 82, and the imaging unit 83 are housed in a light-shielding housing. The drug holding unit 81 is positioned between the illumination unit 82 and the imaging unit 83, has a light-transmitting portion that can hold drugs, and is rotatable by the rotating support unit.

The invention relates to the technical field of nutritional ingredient analysis, and discloses an ingredient metering system for low-protein nutrition balanced catering, and the system comprises a multi-modal data collection module which is used for obtaining the total mass, color and depth images of to-be-detected meals; the visual guidance spectrum analysis module is used for performing instance segmentation on the color image and guiding a spectrum probe to perform targeted scanning on each independent food entity so as to obtain spectrum data; the metabolic twinning and chemical imaging module is used for calculating and summarizing nutritional ingredients of each food entity by fusing spectrum and image information through a metabolic twinning algorithm model; and the individualized self-adaptive calibration module is used for acquiring the real physiological indexes of the user and updating the model parameters according to the metabolic response deviation so as to realize closed-loop calibration. According to the method, the visual guidance spectrum is combined with the metabolic twinborn model, and closed-loop calibration is carried out by utilizing the physiological indexes, so that accurate measurement and individualized analysis of the nutrition of cooked meals are realized.

An infrared (IR) imaging system for determining a concentration of a target species in an object is disclosed. The imaging system can include an optical system including a focal plane array (FPA) unit behind an optical window. The optical system can have components defining at least two optical channels thereof, said at least two optical channels being spatially and spectrally different from one another. Each of the at least two optical channels can be positioned to transfer IR radiation incident on the optical system towards the optical FPA. The system can include a processing unit containing a processor that can be configured to acquire multispectral optical data representing said target species from the IR radiation received at the optical FPA. One or more of the optical channels may be used in detecting objects on or near the optical window, to avoid false detections of said target species.

A method for analysing a specimen in a microscope is provided. The method comprises: acquiring a series of compound image frames using an electron detector, a first X-ray detector, and a second X-ray detector, wherein the first X-ray detector is positioned between the specimen and an electron beam source from which a focused electron beam emerges towards the specimen, and is provided with a filter member interposed between the first X-ray detector and the specimen and adapted to reduce the incidence of electrons on the first X-ray detector, wherein the second X-ray detector is provided with a deflector arrangement configured to reduce the incidence of electrons on the second X-ray detector, and wherein acquiring a compound image frame comprises: causing the focused electron beam to traverse a region of the specimen; monitoring a set of resulting electrons emitted from a plurality of locations within the region of the specimen, using the electron detector, so as to obtain a first image frame, the first image frame comprising a plurality of pixels corresponding to, and having values derived from the monitored electrons emitted from, the plurality of locations; monitoring first and second sets of resulting X-rays emitted from the plurality of locations using the first X-ray detector and the second X-ray detector respectively, so as to obtain one or more second image frames, each comprising a plurality of pixels corresponding to the plurality of locations and having values derived, in accordance with a first criterion, from monitored X rays characteristic of a respective chemical element and emitted from the plurality of locations, wherein the set of electrons and the first and second sets of X-rays are emitted from the specimen substantially simultaneously; and combining the first image frame and the one or more second image frames so as to produce the compound image frame, such that the compound image frame provides data derived from monitored electrons and X-rays emitted from the plurality of locations within the region, and displaying the series of compound image frames on a visual display, wherein the visual display is updated to show each compound image frame in sequence. A system for analysing a specimen in a microscope is also provided.

Provided herein are methods of using a machine learning model to analyze a combination of imaging data and sequencing data to characterize cellular phenotypes and evaluate perturbation effects of drugs and genetic perturbation effects.

In one embodiment, an infrared (IR) imaging system for determining a concentration of a target species in an object is disclosed. The imaging system can include an optical system including an optical focal plane array (FPA) unit. The optical system can have components defining at least two optical channels thereof, said at least two optical channels being spatially and spectrally different from one another. Each of the at least two optical channels can be positioned to transfer IR radiation incident on the optical system towards the optical FPA. The system can include a processing unit containing a processor that can be configured to acquire multispectral optical data representing said target species from the IR radiation received at the optical FPA. Said optical system and said processing unit can be contained together in a data acquisition and processing module configured to be worn or carried by a person.

The invention belongs to the field of micro-plastic pollutant detection, and particularly relates to a method for identifying micro-plastic in blood based on a laser direct infrared imaging system, and the method mainly comprises the following steps: digesting the blood by using 68% concentrated nitric acid; filtering the digested mixed solution through a filter membrane; rinsing the filter membrane; micro plastic particles are enriched; concentrating and transferring the micro plastic particles; dropwise adding a micro-plastic sample on the glass window and drying; a laser direct infrared chemical imaging system is used for identifying the micro-plastic particles in the blood and the apparent characteristics of the micro-plastic particles. The method disclosed by the invention not only can efficiently and simply extract and collect the micro-plastics in the blood, but also can quickly and accurately identify and analyze the types and apparent characteristics of the micro-plastics, effectively makes up the defects of high detection cost, low detection efficiency and the like of a traditional detection method, has a great application potential in detection of the micro-plastics in the blood of the living body, and has a wide application prospect. The important support is provided for comprehensively analyzing the health risk of the micro-plastic to the life organism.

Systems and methods for detecting photothermal effect in a sample are described herein. In these systems and methods, a pump source is configured to generate a pump pulse train, a probe source is configured to generate a probe pulse train and is synchronized with the pump pulse train, and a camera collects the resulting data. The camera is configured to collect a first signal corresponding to a hot frame, wherein the hot frame includes visible probe beam as modified by a pump beam and a second signal corresponding to a cold frame, wherein the cold frame includes visible probe beam that has not been modified by a pump beam. A processor can subtract the second signal from the first signal to detect the photothermal effect.

A method for infrared chemical imaging through nondegenerate two-photon absorption includes a step of providing pulsed or continuous wave radiation having pumping photons at near-infrared wavelength and providing pulsed or continuous wave radiation that having mid-infrared photons at a mid-infrared wavelength with peak intensities less than 50 W / cm2. The mid-infrared photons are directed onto a target sample. The method also includes a step of spatially and temporally overlapping the mid-infrared photons with the pumping photons. The mid-infrared photons and the pumping photons are directed onto a camera having an array or matrix of imaging devices. Characteristically, the sum of photon energy for each temporally and spatially overlapping mid-infrared photons and pumping photons is greater than or equal to the bandgap energy.

Embodiments disclosed include methods and apparatus for Fluorescent Enhanced Photothermal Infrared (FE-PTIR) spectroscopy and chemical imaging, which enables high sensitivity and high spatial resolution measurements of IR absorption with simultaneous confocal fluorescence imaging. In various embodiments, the FE-PTIR technique utilizes combined / simultaneous OPTIR and fluorescence imaging that provides significant improvements and benefits compared to previous work by simultaneous detection of both IR absorption and confocal fluorescence using the same optical detector at the same time.

The present invention provides a method for spectroscopic imaging by illuminating a sample with a short infrared pulse, directing one or more probe beams to the sample, and measuring light that is reflected, transmitted, or re-emitted inelastically, where a spectrum is collocated by varying the wavelength of the infrared pulse and an image is collected by moving the sample and probe beam relative to each other as the steps are repeated multiple times to create a multidimensional spectroscopic image of the sample. Also disclosed are the related methods for analyzing the data and the related system for spectroscopic imaging.

Methods and apparatus for spectroscopy from nanoscale to millimeter scale and imaging techniques, including atomic force microscopy, infrared spectroscopy, confocal microscopy, Raman spectroscopy, and mass spectroscopy. For infrared spectroscopy, the sample is illuminated with infrared light and the resulting sample deformation is read out with a focused UV / visible beam and / or AFM tip. The combination of the two techniques provides fast and large area measurement scans using the UV / visible light and high resolution measurements using the AFM tip. The methods and apparatus also include the ability to analyze the light reflected / scattered from the sample by a Raman spectrometer for supplemental analysis by Raman spectroscopy.

The invention relates to the technical field of pharmaceutical analysis, in particular to a crystal form detection method for Pinefibrate in Pinefibrate tablets, which comprises the following steps: acquiring a sample to be detected from the Pinefibrate tablets; detecting the sample to be detected by using a chemical imaging method based on a vibration spectrum to obtain spectral data; and comparing the spectral data with the spectral data of the known crystal form of Pinefibrate, and identifying the crystal form of Pinefibrate. The method for detecting the crystal form of the pterofibrate in the pterofibrate tablet has the beneficial effects that a Raman spectrometer is used for qualitatively testing the crystal form of a pterofibrate bulk drug in a preparation, complicated pretreatment on a sample is not needed, the risk of crystal transformation possibly caused by pretreatment is avoided, and the sample loading process of the sample is simplified. Meanwhile, the sample is directly scanned and imaged, the position of the pterofibrate bulk drug is accurately positioned, the interference of auxiliary materials is reduced, and the crystal form of pterofibrate in the preparation is rapidly measured.

An infrared (IR) imaging system for determining a concentration of a target species in an object is disclosed. The imaging system can include an optical system including a focal plane array (FPA) unit behind an optical window. The optical system can have components defining at least two optical channels thereof, said at least two optical channels being spatially and spectrally different from one another. Each of the at least two optical channels can be positioned to transfer IR radiation incident on the optical system towards the optical FPA. The system can include a processing unit containing a processor that can be configured to acquire multispectral optical data representing said target species from the IR radiation received at the optical FPA. One or more of the optical channels may be used in detecting objects on or near the optical window, to avoid false detections of said target species.

The invention relates to the technical field of computer vision and environmental monitoring, and discloses a microplastic component rapid identification method based on multi-modal data driving, and the method comprises the steps: firstly obtaining a morphological sequence image and a chemical component map of microplastic, and constructing a standardized three-dimensional voxel data set through metadata alignment; then constructing an uncoupled training set and executing three-dimensional data enhancement based on spatial transformation; training a component identification network by using the enhanced data, and establishing a mapping relation between the micro-plastic three-dimensional morphological characteristics and chemical components; and finally, acquiring a morphological image of a sample to be detected, inputting the morphological image into the network, and outputting a three-dimensional segmentation mask with a chemical component code. According to the method, the deep relation between forms and components is excavated, so that the chemical components and three-dimensional distribution of the micro-plastics are quickly and accurately identified only according to low-cost morphological images, the problems of high cost and low speed of traditional chemical imaging are solved, and the method is suitable for efficient monitoring of environmental micro-plastic pollution.

The invention relates to a single pulverized coal particle three-dimensional chemical imaging method which comprises the following steps: cutting a single pulverized coal particle to obtain pulverized coal slices and a slice sequence of the pulverized coal slices, and performing two-dimensional chemical imaging on each pulverized coal slice based on a microscopic confocal Raman spectrum analysis method to obtain a two-dimensional chemical imaging database of the single pulverized coal particle, and reconstructing a three-dimensional chemical model of the single pulverized coal particles according to the slice sequence and the two-dimensional chemical imaging database. According to the method and the device, the problem of low three-dimensional chemical imaging precision of the single pulverized coal particles is solved, the two-dimensional images of the slices are obtained by carrying out microscopic confocal Raman spectrum analysis on the slices, and the three-dimensional particle model is reconstructed according to the two-dimensional images of the slices and the sequence of the slices, so that the three-dimensional imaging of the solid particles under the high-precision micron scale is realized.

The invention belongs to the technical field of multi-modal combined imaging, and discloses a multi-modal combined imaging method and platform for shale nano-pore three-dimensional reconstruction and connectivity quantitative evaluation, and aims to realize shale organic pore and inorganic pore structure three-dimensional and quantitative characterization based on FIB-SEM and X-CT. High-resolution imaging and chemical imaging technologies are used for revealing the occurrence state of kerogen in mineral particles or at the boundaries, the mineral surface catalyzes organic matter to generate hydrocarbon, and the pore evolution mechanism is regulated and controlled.

The application discloses a self-powered photoelectrochemical imaging system and a preparation method thereof, and belongs to the technical field of photoelectric detection and imaging. The imaging system comprises a flexible substrate, a liquid metal electrode array formed on the surface of the flexible substrate, a photoelectric detection unit array composed of a vanadium oxide photosensitive layer formed on part of the surface of the liquid metal electrode array and a natural gallium oxide counter electrode layer formed on the rest of the surface of the liquid metal electrode array, the vanadium oxide photosensitive layer and the gallium oxide counter electrode layer are arranged alternately and form a planar heterojunction, the planar heterojunction is used for realizing self-power supply of the system, a solid electrolyte layer is filled between each unit of the photoelectric detection unit array, and an encapsulation layer is wrapped outside each functional layer. High-definition imaging in the visible light region can be realized, and the application is suitable for high-precision detection scenes such as biomedical micro-imaging and precision industrial detection.

The instant disclosure provides for medical imaging systems that may be used in conjunction with an intraoperative medical device, such as an endoscope. Generally, the disclosed medical imaging systems include an illumination source configured to generate illuminating photons for illuminating a biological sample. An optical signal modulator is configured to separate one or more of the illuminating photons and photons that have interacted with the biological sample into a first optical signal having first multi-passband wavelengths and a second optical signal having second multi-passband wavelengths. At least one detector is configured to detect one or more of the first optical signal and the second optical signal and generate at least one image data set. A processor is configured to analyze the at least one image data set. In some embodiments, the processor is configured to differentiate between structures of the biological sample, such as between an ureter and surrounding tissue.

The application discloses a CARS-LA-ICP-MS combined multi-mode analysis system, which comprises a fusion optical path module, a mass spectrum analysis gas path module, a three-dimensional moving table, a CARS signal acquisition system and a computer control system; the fusion optical path module comprises a laser source, an optical parametric amplifier, an optical pulse time delay system, three dichroic mirrors, a light beam adjusting system, a galvanometer, a moving objective and a camera; the mass spectrum analysis gas path module comprises a carrier gas supply device, a multi-mode analysis sample pool and a mass spectrum analysis device; the multi-mode analysis sample pool comprises an upper cover, a lower cover and a side wall shell. The application discloses another aspect of an analysis method, which comprises initialization setting, CARS analysis, mass spectrum analysis task parameter setting, laser ablation mass spectrum analysis and data synthesis. Through coaxial optical path design and intelligent algorithm, the application realizes deep fusion of chemical imaging and element analysis, and provides a new research tool for the fields of life science, material science and the like.

The present invention discloses an in-situ chemical imaging method and system for soil earthworm haptospheres, belonging to the field of agricultural experimental technology. The method comprises: S1, preparing an earthworm digging box and filling it with soil; S2, selecting suitable earthworms and placing them in the soil for cultivation, so that sufficient earthworm pores are generated in the soil; S3, sequentially placing a filter membrane, a DGT adsorption membrane, and a pH plane photoelectrode membrane on the soil profile of the earthworm haptosphere to be imaged, so that the three membranes can fully adhere to the soil profile of the earthworm haptosphere, applying ultraviolet light under light-proof conditions, and cultivating for a period of time; capturing the fluorescence signal emitted by the pH plane photoelectrode membrane to obtain a pH fluorescence image; S4, coloring the DGT adsorption membrane; S5, capturing the color image of the DGT adsorption membrane to obtain a DGT image, performing image processing on the DGT image and the pH fluorescence image, and obtaining a spatial distribution image of available phosphorus and pH in the earthworm haptosphere. The present invention can solve the problem of difficulty in in-situ detection of biogeochemical cycle processes driven by earthworm activity in soil.

The application relates to the technical field of computer vision and environment monitoring, and discloses a microplastic component rapid identification method based on multi-modal data driving, which comprises the following steps: firstly, acquiring morphological sequence images and chemical component maps of microplastics, and constructing a standardized three-dimensional voxel dataset through metadata alignment; then, constructing a non-coupling training set and performing three-dimensional data enhancement based on spatial transformation; training a component identification network by using the enhanced data, and establishing a mapping relationship between the three-dimensional morphological characteristics of the microplastics and the chemical components; finally, collecting morphological images of samples to be measured and inputting the images into the network, and outputting three-dimensional segmentation masks with chemical component codes. The application realizes rapid and accurate identification of the chemical components and three-dimensional distribution of microplastics only according to low-cost morphological images by mining the deep connection between the morphology and the components, solves the problems of high cost and slow speed of traditional chemical imaging, and is suitable for efficient monitoring of environmental microplastic pollution.

In one embodiment, an infrared (IR) imaging system for determining the concentration of a target substance in an object is disclosed. The imaging system may include an optical system that includes an optical focal plane array (FPA) unit. The optical system may have components that define at least two optical channels that are spatially and spectrally different from each other. Each of the at least two optical channels may be positioned to transmit IR radiation incident on the optical system toward the optical FPA. The system may include a processing unit that includes a processor that may be configured to acquire multispectral optical data representative of the target substance from the IR radiation received at the optical FPA. The optical system and the processing unit may be included together in a data acquisition and processing module that is configured to be worn or carried by a person.

The present invention belongs to the technical field of single-cell chemical imaging, and specifically is a stimulated Raman microscopy imaging device combined with optical tweezers. The device of the present invention includes a laser optical tweezers optical path, a Raman imaging optical path, and a microscope; the optical tweezers optical path couples the laser to the microscope objective through a dichroic mirror to achieve strong focusing and form a strong gradient force optical trap; the Raman optical path contains two pulsed lights that are synchronized in time domain and overlap in space. The two pulsed lasers are scanned by a scanning galvanometer and then coupled to the microscope by the optical path system to achieve two-dimensional scanning of the light beam on the sample; the optical tweezers use a position-sensitive detector to achieve real-time recording of the position of samples such as cells, and Raman imaging uses a photomultiplier tube or a photodetector after phase-locked amplification and output. The co-localized Raman light source can achieve chemical imaging of multiple components of the sample by scanning. The invented device can be used to perform Raman co-localization imaging of multiple chemical components in cells, and has important applications in the fields of cell component analysis and component distribution measurement.