207 results about "Cellular imaging" patented technology

The invention discloses an image processing method and device, electronic equipment, a storage medium and a computer program product, and the method comprises the steps: obtaining a first image and a second image; based on a first mapping relation, the second image is converted into a first file, and the first mapping relation represents a coordinate mapping relation of corresponding pixels between an output image of the cell imaging system and a display interface of the cell cutting system; the first mapping relation is determined on the basis of the position of a preset positioning graph in the output image and the position of the preset positioning graph on the display interface, and points determined on the basis of the cell contour in the second image are described in the first file; and outputting the first image and the first file to a cell cutting system, so that the cell cutting system cuts an imaged cell in the first image based on the first file.

According to the cell fixing liquid, phenol oxidase is used for catalyzing phenol to be oxidized into a quinone structure, the quinone structure reacts with protein amido to form light covalent immobilization, cell immobilization is achieved, a stable hydration environment and membrane protection are provided through the ionic liquid solvent choline dihydrogen phosphate, a phosphate-free buffer system is provided, and cell immobilization is achieved. Therefore, a novel cell fixing liquid is obtained, the cell structure integrity required by cell imaging analysis is effectively reserved, meanwhile, cell staining fluorescence signal interference is avoided, and therefore the accuracy of cell counting and cell imaging analysis is ensured.

Described herein are techniques for modulating the treatment of cell cultures having cells corresponding to different cell categories. Some techniques may be used with a cell imaging and incubation system that includes an imaging sensor configured to obtain an image of the culture and an incubator configured to incubate the culture. Adjustment of processing of the culture may be based on processing of an image of the culture obtained by an imaging sensor of the system. The processing may include segmenting an image of the culture into a plurality of image segments by assigning individual pixels of the image to corresponding cell categories. According to some embodiments, the techniques include determining an amount of the culture corresponding to a particular cell category based on the image segments. The amount of the culture corresponding to the particular cell category may provide information for modulation of the treatment of the culture.

The invention discloses a preparation method of photoresponse COFs coated CeO2 nano-enzyme, and relates to the field of nano-materials. According to the invention, FePc, TAPP and 2, 5-dihydroxy-1, 4-phthalaldehyde are used for creating a core-shell material CeO2 (at) FePC / por-COF of photoresponse peroxidase based on COFs (Covalent Organic Frameworks), and the core-shell material CeO2 (at) FePC / por-COF is used for preparing the photoresponse peroxidase based on COFs. According to the invention, the highly ordered structural characteristic of the COFs material is utilized, so that the problem of fluorescence quenching caused by aggregation of traditional micromolecule photosensitizers (such as porphyrin, phthalocyanine and the like) in the use process is effectively solved. According to the present invention, the photoresponsive peroxidase activity is significant, and the chromogenic substrate of tetramethyl benzidine (TMB) can be effectively catalyzed; fePc is integrated into a COFs structure, so that the photoresponse activity of the COFs under near-infrared 808 nm is enhanced, and the COFs have excellent reactive oxygen species (ROS) generation capacity, and have great potential in the aspects of photodynamic therapy, antibacterial treatment, cell imaging and the like in biomedical application.

The invention belongs to the technical field of single cell imaging and analysis, and provides an unmarked single cell two-dimensional light scattering imaging modal amplification method and system. The method comprises the following steps: acquiring a two-dimensional light scattering image of an unmarked single cell, and preprocessing the two-dimensional light scattering image; according to the proposed fusion condition adversarial loss LcGAN, L1 distance loss LL1 and a new imaging modal amplification model of perception loss # imgabs0 # based on a VGG network, generating a corresponding single-cell bright field image and a fluorescence image by using the processed light scattering image, and realizing amplification of a single-cell multi-modal image; multi-modal fusion and classification identification are carried out on the single cell two-dimensional light scattering image, the corresponding bright field image and the fluorescence image, so that the cancer single cell analysis accuracy is improved; the method can generate the corresponding single-cell bright field image and fluorescence image according to the unmarked single-cell two-dimensional light scattering image, is especially suitable for diagnosis and classification of cancer cells, and facilitates promotion of biomedical research and clinical application.

Devices, systems, and methods are provided for in vivo fluorescence imaging. Disclosed herein is an implantable miniature fluorescence imager on a chip having a custom imaging array with angle selective gratings, fiber optics, or microcollimators for image deblurring, and optical filters that can be tuned to image fluorescence from multiple fluorophores simultaneously. Power is supplied by an on-chip power source or transmitted to the chip from an external transducer such as an ultrasound transducer, electromagnetic transducer, inductive transducer, or radiofrequency transducer. Wireless communication may be provided by electromagnetic or ultrasound links to the device. The function of a fluorescence microscope is provided in a millimeter-scale device that can be readily implanted in tissue and used to image fluorescently labeled cells in vivo. The small size of the fluorescence imager makes possible sustained in vivo imaging with real-time monitoring of multiple cell types within Shifting the dynamic diseased tissue or a tumor.

The invention discloses a vesicle transportation path prediction system based on artificial intelligence, and relates to the technical field of vesicle transportation, which comprises the following steps of: accurately extracting a complete time sequence movement track of a single vesicle before and after disturbance by virtue of fluorescence living cell imaging under a disturbance experiment condition in combination with a target detection model YOLOv8 and a depth correlation tracking algorithm DeepSORT; the problem that perturbation conditions are insufficiently perceived is solved, through a multi-modal data matrix, the model has the time sequence perceiving capacity for changes of a local space structure where vesicles are located, so that the sensitivity and modeling capacity of the model for track variation in a complex environment are remarkably improved, deep fusion of multi-modal features is achieved by building a double-branch fusion model, and the accuracy of the model is improved. The problem that an existing prediction model cannot actively recognize and correct prediction deviation is effectively solved, and the stability and the actual usability of the system are improved.

The invention relates to the technical field of fluorescence imaging, in particular to a derivative of triphenylamine and thiazole salt, a fluorescent probe as well as a preparation method and application of the fluorescent probe. The fluorescent probe disclosed by the invention is a derivative of triphenylamine and thiazole salt. The structure of the fluorescent probe contains a positive charge, and the internal environment of the mitochondria is a negative charge. When the fluorescent probe provided by the invention is used in cell imaging, the fluorescent probe can penetrate through a cell membrane to enter living cells and is adsorbed on mitochondria under the attraction of negative charges in the mitochondria. In the state, after the fluorescent probe is excited to generate fluorescence reaction based on a specific light field, fluorescence imaging of the cells can be realized, so that the internal change process of mitochondria can be observed through real-time fluorescence imaging. The fluorescent probe disclosed by the invention has relatively small cytotoxicity and relatively good photobleaching resistance, can specifically target mitochondria and can realize long-time fluorescence imaging, so that the fluorescent probe disclosed by the invention has relatively good advantages in cell imaging.

According to the invention, a collaborative deformable mirror, a two-dimensional galvanometer scanning system and a high-precision three-dimensional displacement sample table are designed, and rapid tracking and spot scanning imaging of a freely moving sample are completed. And under the condition of single cell analysis resolution, the tracking range is large, and the imaging speed is high. As the galvanometer controls the deflection angle through the driving voltage, the propagation path of the imaging light path can be efficiently adjusted in real time. Therefore, the tracking response speed based on the galvanometer scanning system is obviously superior to that of a traditional mechanical displacement compensation mode. The displacement is compensated through the sample table, and the tracking range is expanded. The displacement compensation process adopts a sample motion trail pre-judgment algorithm, is carried out in real time and is small in compensation amount, and rapid response of the system is ensured; displacement compensation and galvanometer tracking work cooperatively, the tracking speed is optimized, and spot scanning imaging is completed at the same time. Axial scanning is completed by modulating excitation light wave front through a deformable mirror. The system is integrated with a light stimulation module, wavefront phase modulation is carried out on stimulation light by utilizing a spatial light modulator, single-point or multi-point focusing is realized, single or multiple neurons can be accurately controlled, and the system has important significance on research of neuroscience.

A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

The invention discloses a thiophene site specific modified TAE material as well as a preparation method and application thereof, and relates to the technical field of organic optical functional materials. A main body structure of the TAE material specifically modified by thiophene sites is tetraarylethene, thiophene substitution exists on the tetraarylethene, and R groups are introduced to the tail end of thiophene, or the TAE material specifically modified by the thiophene sites is obtained. The invention also provides an application of the TAE material or the photochemical sensor specifically modified by the thiophene site in photochromism or mechanochromism or cell imaging or safe ink or anti-counterfeiting. According to the invention, through conjugate coupling of a thiophene ring and an ethylene bridged benzene ring, a diarylethene functional unit with light-induced isomerization capability is formed, and the aggregation-induced emission (AIE) performance of the material is remarkably improved while the light response activity is realized. The synergistic effect endows the material with revolutionary potential in the fields of light-operated bimodal patterning technology and multi-dimensional anti-counterfeiting application.

The utility model discloses a miniature cell culture chamber suitable for ultrahigh-flux living cell imaging. The miniature cell culture chamber comprises a culture box with an opening at the front end, a culture plate is connected in the culture box in a sliding manner; a plurality of imaging grooves are formed in the culture plate; an adapter for placing a culture vessel is arranged at the top of the culture plate; the adapters and the imaging grooves are the same in number and are arranged in a one-to-one correspondence mode. A CO2 inlet and an N2 inlet are formed in the top of the incubator; an air filter, a heating module and a humidifier are arranged in the incubator; a lamp panel is arranged at the top of the incubator; lED lamps are distributed at the bottom of the lamp panel in an array mode. A transparent imaging plate is arranged at the bottom of the incubator. Through cooperation of the culture plate, the imaging groove, the imaging plate, the adapter, the LED lamp and other components, the living cell ultra-high-flux imaging device can achieve living cell ultra-high-flux imaging, is convenient to operate, high in space utilization rate and small in internal space, and can guarantee uniformity of internal gas, temperature and humidity.

The invention discloses a difunctional near-infrared fluorescent probe as well as a synthesis method and application thereof. The structural formula of the fluorescent probe is shown as a formula (I). The fluorescent probe DTPD provided by the invention can be used for fluorescent quantitative detection of hypochlorous acid and viscosity, and the fluorescent probe can realize detection of hypochlorous acid with high selectivity in an aqueous solution and is not interfered by other coexisting substances. Besides, the near-infrared fluorescent probe provided by the invention has relatively strong organism penetrability in a near-infrared region, can also reduce the interference of autofluorescence of organisms, and has a good cell imaging effect. And # imgabs0 #.

The invention discloses a monoclonal cell for expressing HPV (human papilloma virus) 18 type E6 protein and application of the monoclonal cell in construction of an HPV tumor animal model, and relates to the technical field of HPV tumor animal models. A monoclonal cell which can stably express target protein and is good in tumor formation effect is obtained through a limited dilution method and single cell imaging sorting identification, and after an animal model is inoculated with the medicine or vaccine composition, the medicine or vaccine composition has an obvious inhibition effect on tumors; an animal model can be induced to generate high-level humoral immunity, and high-level specific CD4 + T cell and CD8 + T cell immune response can be generated. Compared with a TC-1 cell, the monoclonal cell provided by the invention has more remarkable tumorigenicity and can better mediate the growth of tumors in animals. The monoclonal cell obtained by screening has a good application prospect in screening a pharmaceutical composition or a vaccine composition for preventing and / or treating HPV, and can be used for evaluating a therapeutic vaccine for targeting HPV18.

A computer-implemented method for identifying bladder cancer cells includes: receiving a microscopic image of a specimen imaging a plurality of cells; analyzing a quality of the microscopic image; classifying the cells imaged on the microscopic image to indicate potentially cancerous cells; analyzing the whole microscopic image to determine an overall probability that the microscopic image comes from a potentially cancerogenous patient, based on distribution of the potentially cancerogenous cells; presenting, on a single screen of a graphical user interface of a computer system: an overall image representing box and a plurality of cell identification boxes; receiving on said single screen an expert input; repeating the step of analyzing; and generating a final report that identifies an overall probability that the microscopic image comes from a potentially cancerogenous patient.

The invention relates to the technical field of cell manipulation and analysis, and relates to an automatic single cell continuous capturing and screening system and a use method thereof. Comprising a bias liquid storage module, a cell injection module, a micro-fluidic chip, a cell capturing module, a cell screening module and an imaging system, the bias liquid storage module, the cell injection module, the cell screening module and the cell capturing module are connected with the micro-fluidic chip; the bias liquid storage module is used for generating bias flow; the cell injection module is used for providing cells; the micro-fluidic chip is used for capturing and screening cells; the cell capturing module is used for capturing cells; the cell screening module is used for screening cells; and the imaging system is used for acquiring captured cell images and performing visual analysis. Micro-fluidic pipeline double-bias-flow stable cell flow sample introduction is designed, a high-speed electromagnetic valve is matched to realize rapid control of a gas path on cell capturing and screening channel fluid, and a high-speed camera is combined to analyze cell positions in different areas in real time, so that automatic single cell continuous capturing and screening are realized.

The invention discloses an optical fiber micro-beam probe and system capable of achieving synchronous imaging of cell morphology and mechanical characteristics, the optical fiber micro-beam probe comprises a single-mode optical fiber, a Bragg grating, a supporting column, a micro-beam and a nanometer needle tip, the single-mode optical fiber comprises a fiber core and a wrapping layer outside the fiber core, and the Bragg grating is located in the fiber core of the single-mode optical fiber; the first end of the supporting column is fixed to the second end face of the single-mode optical fiber, one end of the micro-beam is fixed to the second end of the supporting column, and the nanometer needle tip is located on the surface of the other end of the micro-beam. The metal film is introduced to enhance the reflectivity of the micro-beam, and the nano needle tip is introduced to improve the cell morphology and mechanical imaging resolution. An optical fiber FPI sensitive external weak force is formed on the end face of an optical fiber by adopting a micro-beam probe, resonance of the micro-beam probe is excited by light driving, the resonance is influenced by the weak attractive force on the surface of a cell, the change of information such as resonant frequency and amplitude is monitored, and synchronous imaging of the morphology and the mechanical property of the living cell is realized. Miniaturization, integration and high efficiency of an imaging system are facilitated, and a new method is provided for cell imaging in the fields of biomedicine and the like.

The present disclosure is generally directed to anchoring bridge nucleic acid probes in a polymer matrix, which are configured to hybridize to cellular nucleic acid, embedding in a polymer matrix, clearing cellular components including the cellular nucleic acid, contacting the anchored bridge probes with primary probes that hybridize with the bridge probes and imaging. The disclosure describes methods for imaging cellular RNA in an RNase insensitive method.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

The invention belongs to the field of molecular fluorescent probes, and particularly relates to a near-infrared frequency up-conversion fluorescent probe, a benzopyranium salt compound as well as a preparation method and application of the benzopyranium salt compound. According to the invention, benzopyranonium salt compounds derived from natural materials such as anthocyanin are used as a parent body, an electron-donating group is introduced as a donor, and then the small organic molecule with a D-pi-A structure is synthesized through a Knoevenagel condensation reaction, and the molecule has the effect of exciting and positioning mitochondria by down-conversion of 650 nm, and also has the effect of reducing mitochondria by down-conversion of 650 nm. And the fluorescent probe also has the characteristic of exciting up-conversion fluorescence targeting mitochondria at 760 nm, so that the fluorescent probe can be used as a fluorescent probe in cell imaging. The material has stronger penetrating power of biological tissues during fluorescence imaging, so that the material has a better fluorescence imaging effect. The scheme of the invention also provides a related process capable of stably synthesizing the fluorescent probe and the benzopyranium salt compound serving as a core raw material of the fluorescent probe, so that the fluorescent probe has higher practical value.

Methods and processes disclosed herein generally involve training a machine learning model to identify a condition active in changing a cellular phenotype. In particular, cells having the cellular phenotype can be cultured in wells having a reference condition that changes the cellular phenotype, along with a background compound to provide a reference plurality, and cells having the cellular phenotype can be cultured in wells having a background compound to provide a non-reference plurality. A staining and imaging assay can be performed on the reference and non-reference pluralities after incubation, and the images are applied to a machine learning model to classify the results into activity data, to identify a "hit" activity plurality as corresponding to the reference plurality, and a "non-hit" activity plurality as corresponding to the non-reference plurality. The machine learning model can be used in a process to identify a test condition predicted to be active in changing a cell line phenotype.

The invention provides a peripheral blood cell morphological analysis system and a heart failure degree judgment method, and the method comprises the steps: taking the peripheral blood cell morphological analysis system as the basis, obtaining the fluorescence signal, intensity, phase, bright field and dark field images of red blood cells, building the relation between the heart failure degree and red multi-dimensional features, and determining the heart failure degree of the red blood cells; and constructing, training and optimizing an erythrocyte morphological feature extraction network model based on machine learning. On the basis, a red blood cell characteristic image feature extraction network model with a fluorescence label is adopted to analyze a red blood cell image obtained by detecting a peripheral blood sample by a multi-parameter flow cytometry imaging system, the correlation between the characteristics of red blood cells in the peripheral blood sample and the heart failure degree is analyzed, and the heart failure degree is judged quickly and precisely.

The invention provides a novel borate tricyclic hemicyanine dye and a synthesis method thereof, and the dye has excellent fluorescence performance and is suitable for biological detection and medical imaging. Two synthesis routes are provided, 5-formylfuran-2-boric acid is used as a raw material, and the target dye is efficiently synthesized through different chemical reaction routes. According to the route I, hemicyanine and indole quaternary ammonium salt are condensed to form a hemicyanine intermediate, and the hemicyanine intermediate reacts with o-aminophenol or a derivative thereof; the route II is firstly condensed with aniline or a derivative thereof to form an imine intermediate, and then a final product is synthesized through a substitution reaction. The synthesis steps are simple, conditions are mild, and large-scale production is facilitated. The synthesized dye has good water solubility and biocompatibility, is adjustable in fluorescence characteristic, and is suitable for various biomedical applications. The embodiment and attached drawings show the synthesis process, the fluorescence property and the application thereof in cell imaging in detail, and show the important value in scientific research and practical application.

The purpose of the present invention is to provide a method for preparing resveratrol carbon dots with good biocompatibility and its application, which belongs to the technical field of nanocomposite materials. Citric acid and resveratrol are dissolved in deionized water, reacted at 180°C for 18h, and after the reaction is complete, the temperature is gradually reduced at different temperatures, and finally cooled to room temperature. The generated carbon dot solution is placed in a dialysis bag for dialysis, centrifuged, filtered through a filter membrane, and freeze-dried to finally obtain resveratrol carbon dots. Studies have shown that the CDs have good biocompatibility in cells and have good performance in cell fluorescence imaging. This achievement has guiding significance for the preparation of composite nanomaterials and cell imaging. In addition, the preparation process of the present invention is simple, low in toxicity, low in cost, and strong in applicability.

Facet-based DNA polyhedral nanostructures with single strands of DNA for each polyhedron face. The DNA-based polyhedral nanostructures may be configured for targeted drug delivery and cell imaging for therapeutic, diagnostic and analytical diagnostic applications. The DNA polyhedral nanostructures adopt platonic icosahedral and dodecahedral configurations resembling the natural shapes of viral nucleocapsid proteins. The DNA polyhedral nanostructures address multiple technical challenges in the biomedical and biotechnological fields, including reducing the cost and complexity associated with conventional DNA origami systems, enhancing the sensitivity of diagnostic platforms, improving tracer delivery and signal clarity in PET and MRI, providing structural stability for nanostructure and payload integrity, and improving the resolution of Cryo-EM imaging of small molecules.

1. Name of the product in this design: Integrated instrument for in situ cell culture and dynamic imaging. 2. Purpose of this design: for in situ cell culture, and to simultaneously perform real-time dynamic imaging of cells from the bottom. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention belongs to the technical field of cytobiology and optical imaging, and particularly relates to a protein point recognition and analysis method and device based on real-time cell imaging. The protein point recognition method based on real-time cell imaging comprises the following steps: S11, acquiring a cell fluorescence image, performing denoising processing on the cell fluorescence image by adopting a non-local mean denoising algorithm, and removing background nonuniformity by using morphological opening operation to obtain a preprocessed image; and S12, dynamically calculating a threshold value of the preprocessed image based on local image intensity, carrying out binaryzation to obtain an image with a background and a foreground, carrying out morphological operation on the image to remove noise and artifacts, and carrying out image region segmentation on the image to obtain an image with a plurality of protein points. According to the method, a non-local mean denoising algorithm and morphological background correction are combined, so that the quality of the low-signal-to-noise-ratio cell fluorescence image is remarkably improved.

The present invention provides a D-D-π-A type organic photosensitizer and a synthesis method and application thereof, belonging to the field of photosensitizer and photodynamic therapy application of tumors. The present invention uses triphenylamine and carbazole as electron donors, malononitrile as an electron acceptor, and thiophene as an electron π bridge to synthesize an AIE type photosensitizer with mitochondrial targeting function through a series of simple organic reactions. The AIE type photosensitizer can be used in the synergistic photodynamic and ferroptosis treatment of tumors. The photosensitizer can realize tumor cell imaging and target mitochondria, and can selectively exert photodynamic and ferroptosis effects on tumor cells. It has good therapeutic effect and biosafety, and has strong application prospects in non-invasive tumor treatment.

The application discloses a cell cutting method and device, electronic equipment and computer program product, wherein the method comprises: acquiring a plurality of first files; each of the plurality of first files is used for a cell cutting system to cut a group of cells in a plurality of groups of cells imaged in a first image; the group of cells comprises one or more cells; the first image is determined based on an image output after a cell imaging system performs cell imaging processing on a target tissue; based on the plurality of first files, a second file is generated; the second file contains first data for describing a cutting path of each cell in a plurality of groups of cells corresponding to the plurality of first files; and the second file is output to the cell cutting system, so that the cell cutting system cuts a plurality of groups of cells imaged in the first image based on the second file.

This invention relates to the field of organic small molecule fluorescent probe technology, specifically to a neuron-specific dual-targeting fluorescent probe targeting the AChR of cholinergic neurons and its applications. The fluorescent probe, whose structure follows general formula I, is characterized by its dual-targeting structure, making it particularly suitable for the identification and labeling of cholinergic neurons. Furthermore, this fluorescent probe exhibits significant advantages in multiple application scenarios, such as cell imaging, protein and organelle labeling, tumor-specific recognition, and photodynamic therapy, with its potential application value in neuroimaging being particularly prominent. This fluorescent probe is expected to advance the development of neurobiology, disease diagnosis, and treatment methods, and has promising application prospects.