96 results about "Cellular imaging" patented technology

According to the cell fixing liquid, phenol oxidase is used for catalyzing phenol to be oxidized into a quinone structure, the quinone structure reacts with protein amido to form light covalent immobilization, cell immobilization is achieved, a stable hydration environment and membrane protection are provided through the ionic liquid solvent choline dihydrogen phosphate, a phosphate-free buffer system is provided, and cell immobilization is achieved. Therefore, a novel cell fixing liquid is obtained, the cell structure integrity required by cell imaging analysis is effectively reserved, meanwhile, cell staining fluorescence signal interference is avoided, and therefore the accuracy of cell counting and cell imaging analysis is ensured.

A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

The invention relates to the technical field of cell manipulation and analysis, and relates to an automatic single cell continuous capturing and screening system and a use method thereof. Comprising a bias liquid storage module, a cell injection module, a micro-fluidic chip, a cell capturing module, a cell screening module and an imaging system, the bias liquid storage module, the cell injection module, the cell screening module and the cell capturing module are connected with the micro-fluidic chip; the bias liquid storage module is used for generating bias flow; the cell injection module is used for providing cells; the micro-fluidic chip is used for capturing and screening cells; the cell capturing module is used for capturing cells; the cell screening module is used for screening cells; and the imaging system is used for acquiring captured cell images and performing visual analysis. Micro-fluidic pipeline double-bias-flow stable cell flow sample introduction is designed, a high-speed electromagnetic valve is matched to realize rapid control of a gas path on cell capturing and screening channel fluid, and a high-speed camera is combined to analyze cell positions in different areas in real time, so that automatic single cell continuous capturing and screening are realized.

The present disclosure is generally directed to anchoring bridge nucleic acid probes in a polymer matrix, which are configured to hybridize to cellular nucleic acid, embedding in a polymer matrix, clearing cellular components including the cellular nucleic acid, contacting the anchored bridge probes with primary probes that hybridize with the bridge probes and imaging. The disclosure describes methods for imaging cellular RNA in an RNase insensitive method.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

1. Name of the product in this design: Integrated instrument for in situ cell culture and dynamic imaging. 2. Purpose of this design: for in situ cell culture, and to simultaneously perform real-time dynamic imaging of cells from the bottom. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application discloses a cell cutting method and device, electronic equipment and computer program product, wherein the method comprises: acquiring a plurality of first files; each of the plurality of first files is used for a cell cutting system to cut a group of cells in a plurality of groups of cells imaged in a first image; the group of cells comprises one or more cells; the first image is determined based on an image output after a cell imaging system performs cell imaging processing on a target tissue; based on the plurality of first files, a second file is generated; the second file contains first data for describing a cutting path of each cell in a plurality of groups of cells corresponding to the plurality of first files; and the second file is output to the cell cutting system, so that the cell cutting system cuts a plurality of groups of cells imaged in the first image based on the second file.

This invention relates to the field of organic small molecule fluorescent probe technology, specifically to a neuron-specific dual-targeting fluorescent probe targeting the AChR of cholinergic neurons and its applications. The fluorescent probe, whose structure follows general formula I, is characterized by its dual-targeting structure, making it particularly suitable for the identification and labeling of cholinergic neurons. Furthermore, this fluorescent probe exhibits significant advantages in multiple application scenarios, such as cell imaging, protein and organelle labeling, tumor-specific recognition, and photodynamic therapy, with its potential application value in neuroimaging being particularly prominent. This fluorescent probe is expected to advance the development of neurobiology, disease diagnosis, and treatment methods, and has promising application prospects.

The invention discloses a targeted KRAS G12C environment-sensitive fluorescent probe as well as a preparation method and application thereof, the fluorescent probe is a compound shown in the following formula or pharmaceutically acceptable salt thereof, and the structural formula is shown in the specification. The invention also specifically discloses a preparation process of the targeted KRAS G12C environment-sensitive fluorescent probe and application of the targeted KRAS G12C environment-sensitive fluorescent probe in preparation of a living cell imaging detection preparation. According to the invention, a known KRAS G12C covalent inhibitor structure is reasonably modified according to a molecular docking mode of the inhibitor and KRAS G12C, and an environment-sensitive fluorescent labeling group is introduced to form the environment-sensitive fluorescent probe with a switch mechanism, so that the environment-sensitive fluorescent probe has the advantages of simplicity and convenience in operation, no need of washing, no need of paraformaldehyde fixation of cells and the like; the invention aims to realize the intuitive visualization of KRAS G12C in living cells / in vivo, and assists in the research and visual application of targeted therapy.

The application discloses a phosphorescent on-off type biological orthogonal iridium complex probe and a preparation and application thereof, and belongs to the technical field of organic photoelectric materials. A benz[h]quinoline-5,6-dione derivative is used as a C^N ligand, the C^N ligand is subjected to a coordination reaction with an iridium trichloride trihydrate to obtain a cyclometalated iridium chloro-bridged dimer, and then the cyclometalated iridium chloro-bridged dimer is subjected to a reaction with an N^N ligand to obtain the iridium complex probe. After the probe is reacted with a biological orthogonal unit, angelica lactone, the excited state energy level of the complex is changed, the photophysical property of the complex is influenced, the phosphorescence is turned on from nothing, and the effect of phosphorescence on-off is achieved. The phosphorescence on-off characteristic makes the probe before and after the reaction with the angelica lactone present obvious luminescent signal ratios. The characteristic can maximally reduce background signal interference, is convenient for cell imaging and biomolecular recognition, and can provide a new train of thought for developing a novel phosphorescent on-off type biological orthogonal material. The application has a good application prospect in the field of biological marking and imaging.

The invention discloses an anti-aging three-dimensional high-uniformity dermis evaluation model based on image analysis, and belongs to the technical field of biological medicine and cosmetic efficacy evaluation. The method comprises the following steps: mixing a prepared fibroblast suspension and a neutralized collagen solution at 0-5 DEG C, inoculating the mixture into a 24-pore plate in a 100-200L / pore manner, standing in an incubator at 37 DEG C for 20-40 minutes to form collagen gel, carrying out suspension culture to obtain a 3D dermal model, and detecting the shrinkage rate of the model and evaluating the anti-aging effect in combination with a high-content cell imaging system. The method overcomes the defect that a traditional method is large in error, an efficient and accurate evaluation system is provided for anti-aging drug screening, and the method has application value in cosmetic effect detection and skin aging mechanism research.

The invention provides a single-component boron-nitrogen-doped dual-emission carbon quantum dot and a preparation method and application thereof.The carbon quantum dot is synthesized through a triaminophenylboronic acid one-step hydrothermal method, 370 nm and 420 nm dual-emission peaks are generated at the same time under 320 nm excitation, the average particle size ranges from 2.0 nm to 2.3 nm, the boron doping amount ranges from 8% to 12%, and the nitrogen doping amount ranges from 2.5% to 4.0%. The carbon quantum dot as a ratiometric fluorescent probe can simultaneously detect Fe < 3 + > and Hg < 2 + >, has detection limits of 0.069 [mu] M and 0.1524 [mu] M respectively, has linear response in a pH range of 8.0-13.0, and is successfully used for cell imaging. The problems that a traditional multi-component probe is complex in preparation and poor in stability are solved, multifunctional integrated detection is achieved, and the probe has wide application prospects in the fields of environmental monitoring and biomedicine.

This invention discloses a method for studying the mechanism by which the mucoprotein channel 1 (TRPML1) regulates the outflow of zinc ions from lysosomes and induces changes in mitochondrial ATP. This method uses 13 nm AuNPs as loading materials to construct a logic-gated sensor for pH and zinc ion regulation within lysosomes and for TRPML1 mRNA in the cytoplasm. It also uses 5 nm AuNPs as loading materials to construct a sensor targeting mitochondrial ATP for cell imaging. This method enables the detection of mRNA and zinc ions in the cytoplasm, lysosomes, and mitochondria. 2+ ATP-associated ATP sensors, Zn 2+ Three sensors—a sensor, an mRNA sensor, and an X-ray sensor—are used simultaneously to image cells, aiming to answer questions about the mechanisms of ATP changes in mitochondria under the regulation of zinc ion efflux from lysosomes by TRPML1.

The application provides a novel ratio near-infrared fluorescent probe for detecting sulfur dioxide, a preparation method and application of the fluorescent probe, the fluorescent probe is Cou-Oxo, the preparation method comprises the following steps: dissolving a compound Cou-CN and a compound 3 in toluene, adding piperidine to catalyze a reaction, refluxing at 114-116 DEG C under a nitrogen atmosphere for 4.8-5.2 hours, and after the reaction is completed, the fluorescent probe Cou-Oxo is obtained through purification.The ratio fluorescent probe has good light emitting characteristics, can effectively detect sulfur dioxide derivatives, has the potential of quantitative detection, and the lower limit of detection is 0.942 muM.The ratio fluorescent probe has a fast response to the detection of sulfur dioxide derivatives, has good light emitting characteristics and stability, and can detect HSO3 ‑ in a physiological pH range of 7-9; the ratio fluorescent probe has lower biological toxicity and is suitable for cell imaging.

This invention provides a benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe for lipid droplets and lysosomes, its preparation method, and its application, relating to the field of fluorescent probes. The preparation method of the benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe includes the following steps: a primary reaction and a secondary reaction. The benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe of this invention can simultaneously target and monitor two organelles (lipid droplets and lysosomes), and exhibits low cytotoxicity and accurate cell localization in HeLa cell imaging experiments; furthermore, it achieves both good polarity response and effective response to intracellular viscosity, demonstrating excellent response performance.

Provided are a cell imaging device and a cell imaging method that can shorten the time period of taking images of cells in a liquid sample, compared with conventional techniques. This cell imaging device 100 introduces a urine sample containing cells into an internal space 11 of a sample cell 10, moves in a second direction at least one of the sample cell 10 and an objective lens 52 while at least one of the sample cell 10 and the objective lens 52 is moved in a first direction, the second direction being different from the first direction, and takes, at a plurality of imaging positions, images of cells contained in the urine sample by means of an imaging unit 50.

The invention discloses an automatic focusing module and an automatic focusing method for high-flux living cell imaging. The automatic focusing module comprises a base; a Z-axis moving mechanism is arranged on the base; a converter fixing seat is arranged on the Z-axis moving mechanism; an electric converter is arranged on the converter fixing seat; the electric converter is provided with a laser automatic focusing sensor and an objective lens. The laser automatic focusing sensor and the objective lens are integrated on the electric converter, when the laser automatic focusing sensor detects the distance of a specimen, the laser automatic focusing sensor does not need to move along the X-axis and the Y-axis, the position of the objective lens only needs to be switched through the electric converter, the Z-axis moving mechanism drives the objective lens to move to a proper position along the Z-axis, and then photographing and imaging can be completed. Errors caused by repeated displacement can be effectively avoided, the imaging effect is improved, the working efficiency can be improved, and the working time can be shortened.

1. The name of the design product: dynamic cell imaging counting analyzer. 2. The use of the design product: for imaging and analyzing the count of cells. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention provides an AIE diagnosis and treatment agent and a preparation method and application thereof.The preparation method includes the steps that a bovine serum albumin solution and an AIE molecular solution are mixed and subjected to ultrasonic treatment, a drug molecular solution is added and stirred, and a copper ion solution is dripped to obtain a material ARC-coated BSA, the material ARC-coated BSA comprises a carrier, the carrier is bovine serum albumin, AIE molecules, drug molecules and copper ions are loaded on the carrier, and the drug molecules and the copper ions are loaded on the carrier. The fluorescent probe is applied to fluorescent quantitative detection of glutathione, cell imaging and distinguishing, in-vivo tumor localization tracing or photodynamic therapy instruments. According to the technical scheme, the in-vivo availability of AIE molecules can be improved, and development of diversified application of AIE materials is promoted.

The present application relates to a kind of small molecule probes and its preparation method and application, the chemical structure of the probe is as shown in the following formula: Wherein, R1 is 1-propyl sulfonic acid or 1-butyl sulfonic acid;R2 independently selected from trifluoromethyl or sulfur pentafluoride group, the substitution position of R2 is the ortho, para or meta of hydroxyl on benzene ring;Preparation method is by the condensation reaction of salicylaldehyde containing fluorine substituent group and indoline, directly introduce containing fluorine substituent group into the spiro-pyran molecule structure with pH response, prepared with 19 F MRI and fluorescence imaging pH-responsive bimodal imaging small molecule probe.Compared with the prior art, the small molecule probe of the present application exhibits significantly different 19 F NMR chemical shift and fluorescence intensity, high-resolution 19 F MRI imaging and fluorescence imaging, greatly improve the sensitivity and accuracy of imaging, has important application prospect in the field of biosensing, cell imaging, medical diagnosis etc.

The invention relates to a method for characterizing images of cell cultures in order to quantify cell parameters. The steps of the method comprise detecting at least one cell parameter or cell marker parameter in an image of cells in culture; constructing a mosaic of the image (squares or rectangles, also referred to as tiles); defining an image with smaller dimensions (also referred to as a density map) by integrating values of the cell parameters or cell marker parameters in each fraction of the mosaic; applying deep learning algorithms, which are implemented for example by a convolutional neural network, to the images generated in step c in order to determine a proximity score for the images of treated cells with respect to images of healthy cells or untreated diseased cells.

The present application relates to a tunable annular diaphragm cell imaging system and a dye-free deep learning identification method, relating to the technical field of optical imaging and image processing, comprising an annular diaphragm illumination module; the present application establishes a quantitative mapping relationship between the outer diameter of the annular diaphragm parameter and the image quality index, and adaptively finds the best bright field and dark field illumination conditions for different cell types, densities and overall profiles and internal structures, so as to obtain an original image with sharp edges and distinct internal structures without using any dye, the improved deep learning segmentation model has a two-way attention gate module, which can capture spatial details and texture semantic information important for cell recognition in parallel and differentially, realize high-accuracy dye-free cell dead or alive automatic identification, make the segmentation result more consistent with the biophysical characteristics of cells, improve the segmentation accuracy and recognition accuracy, and automatically distinguish the state of cells with complex background without fluorescent labeling.

The application belongs to the cross field of coordination chemistry and nanomaterials, and discloses a super-stable strong luminescent N-heterocyclic carbene-protected chiral heterometallic cluster and a preparation method thereof. 58.5 H 59 AuBr4ClCu4F6N8OP (abbreviated as: R / S -NHC py -AuCu4 ‑ Br) belongs to an orthorhombic system, and the space group is a chiral space group P 212121. Another chemical formula of the nanocluster is C 58 H 58 AuCu4F6I4N8P (abbreviated as: R / S -NHC py -AuCu4 ‑ I) belongs to an orthorhombic system, and the space group is a chiral space group I 41. The cluster has a structure that can be stable to 150 DEG C in air. R / S -NHC py -AuCu4 ‑ Br has strong orange luminescence at room temperature in air, and the luminescence quantum yield is 58%. The chiral structure and strong luminescence make it have strong chiral luminescence characteristics, and it can have good application prospects in the fields of holographic projection, information storage and cell imaging.

The invention provides a europium-doped carbon quantum dot fluorescent probe, a preparation method thereof, a tetracycline detection method and a cell imaging method, and belongs to the technical field of fluorescent probes. According to the preparation method, pineapple peel is taken as a carbon source, atomic-scale uniform doping of Eu < 3 + > is realized through a one-step hydrothermal method, ion leakage caused by physical adsorption is avoided, the stability of the probe is improved, and tedious synthesis steps are avoided. The europium-doped carbon quantum dot fluorescent probe has the advantages of high stability, high sensitivity and strong anti-interference performance, and can synchronously realize environmental water sample and cell imaging. The europium-doped carbon quantum dot fluorescent probe shows bimodal fluorescence at 440 nm and 618 nm, the tetracycline detection method is based on a self-calibration mechanism of F618 / F440 ratio signals of the europium-doped carbon quantum dot fluorescent probe, environmental interference is eliminated, and the detection sensitivity is improved. The problems that an existing tetracycline detection material is poor in stability, low in sensitivity and difficult to be compatible with living body imaging are solved.

The application provides an A,A'-dihydroxynaphthaldehyde hydrazone functionalized conjugated pillar[5]arene and a synthesis method thereof. First, a di-m-benzoic acid methyl ester functionalized conjugated pillar[5]arene and hydrazine hydrate are used as substrates to react in anhydrous ethanol solvent to obtain a di-m-benzoic acid hydrazine functionalized conjugated pillar[5]arene; then the di-m-benzoic acid hydrazine functionalized conjugated pillar[5]arene and 2-hydroxy-1-naphthaldehyde are used as substrates to react in anhydrous methanol to obtain the A,A'-dihydroxynaphthaldehyde hydrazone functionalized conjugated pillar[5]arene HGP5. The sensor molecule HGP5 in the application can detect Arg in a "colorimetric fluorescence" dual-channel mode, and can also detect Al 3+ in a super-sensitive mode under fluorescence. In addition, the chemical sensor has potential application in cell imaging, and can detect Al 3+ and Arg in cells. The application provides a new idea for developing a novel supramolecular fluorescent material for detecting Arg and Al 3+ in an aqueous solution.

This invention belongs to the field of fluorescence analysis technology and relates to the application of a sulfur quantum dot fluorescent probe in tryptophan detection. This invention utilizes sulfur quantum dot probes to explore exogenous tryptophan cell imaging technology, studies the role of ultraviolet light in cell imaging, and enhances the fluorescence signal intensity of the sulfur quantum dot fluorescent probe by ultraviolet light irradiation, effectively achieving in vitro quantitative detection of tryptophan and highly sensitive, highly selective in situ detection in living cells. This invention can be used for the detection of tryptophan in vitro and in living cells, and has promising clinical application prospects in the biomedical field.

The application provides a method for correlating gene expression and cell microphenotype based on machine learning, wherein the method comprises: obtaining cell imaging data and spatial transcriptome data, wherein the cell imaging data comprises a nucleus image of a target cell; performing single cell segmentation based on the nucleus image and the spatial transcriptome data by fine-tuning a large model in the field of vision to obtain a cell segmentation image of the target cell; determining a cell mask of the target cell based on the cell segmentation image; quantifying the cell mask to obtain a microphenotype of the target cell; determining gene expression of the target cell based on the cell mask of the target cell and the spatial transcriptome data; and performing correlation analysis on the microphenotype of the target cell and the gene expression of the target cell based on a preset machine learning model to obtain a correlation result of the microphenotype and the gene expression of the target cell.

The invention relates to the technical field of biology, and discloses a recovery liquid for improving recovery efficiency and vitality of organoids, which comprises a metal ion chelating agent, a dissociation agent and a cell protective agent. According to the recovery liquid, matrigel is mildly disintegrated through cooperation of the metal ion chelating agent and the dissociation agent, the organoid can be separated from the matrix within 30 minutes, and the recovery rate of the organoid reaches 90% or above and is far higher than that of a pure mechanical blowing method; the dissociation agent is used for replacing pancreatin and other strong digestive enzymes, and is matched with the cell protective agent, so that the survival rate of the recovered organoid is greater than or equal to 95%, the three-dimensional structure and the intercellular connection are kept complete, and the biological function is real; all the components are clear, the batch is stable, the operation is simple and convenient, the storage is convenient, the culture medium is suitable for passage, collection, downstream cell counting, molecular detection, immunofluorescence, living cell imaging and the like of various sources of organoids such as gastrointestinal tracts, livers and lungs, and the standardization and repeatability of organoid culture are remarkably improved.

This invention discloses a dual-emission silicon quantum dot, its preparation method, and its applications, belonging to the fields of chemistry and nanomaterials science. The present invention uses 3-aminopropyltrimethoxysilane and oreganoic acid as reaction precursors to prepare dual-emission fluorescent silicon quantum dots via a one-step hydrothermal method. Using the technical solution of this invention, dual-emission silicon quantum dots can be prepared in a single hydrothermal reaction, making the synthesis method simple, using readily available raw materials, and inexpensive. The dual-emission silicon quantum dots prepared by this invention exhibit good photostability, low cytotoxicity, and two fluorescence emission peaks, displaying distinct blue and green dual-channel fluorescence in MCF-7 cells, showing potential applications in cell imaging.

The application relates to a stem cell culture density optimization control method and system, and relates to the technical field of stem cell culture, and comprises the following steps: K culture boxes and P culture dishes are configured; when mesenchymal stem cells are cultured, the P culture dishes are randomly and equally distributed to the K culture boxes; P cell imaging sequences in a historical time window are monitored; P real-time cell proliferation rates and morphological characteristics are obtained through analysis; the P culture dishes are similar clustered based on the same; K similar culture dish sets and K cell proliferation rate averages are determined; the K similar culture dish sets are mapped and placed in the K culture boxes; and the K culture boxes are respectively optimized and adjusted in culture environment parameters based on the K cell proliferation rate averages, so that K adaptive environment parameters are obtained, and the K culture boxes in a future time window are controlled. The application solves the problems that in traditional stem cell culture, a unified culture environment is adopted, targeted culture is lacked, and the culture density control precision is low and the cell quality is unstable.