21 results about "Cellular imaging" patented technology

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

This invention discloses a method for studying the mechanism by which the mucoprotein channel 1 (TRPML1) regulates the outflow of zinc ions from lysosomes and induces changes in mitochondrial ATP. This method uses 13 nm AuNPs as loading materials to construct a logic-gated sensor for pH and zinc ion regulation within lysosomes and for TRPML1 mRNA in the cytoplasm. It also uses 5 nm AuNPs as loading materials to construct a sensor targeting mitochondrial ATP for cell imaging. This method enables the detection of mRNA and zinc ions in the cytoplasm, lysosomes, and mitochondria. 2+ ATP-associated ATP sensors, Zn 2+ Three sensors—a sensor, an mRNA sensor, and an X-ray sensor—are used simultaneously to image cells, aiming to answer questions about the mechanisms of ATP changes in mitochondria under the regulation of zinc ion efflux from lysosomes by TRPML1.

Provided are a cell imaging device and a cell imaging method that can shorten the time period of taking images of cells in a liquid sample, compared with conventional techniques. This cell imaging device 100 introduces a urine sample containing cells into an internal space 11 of a sample cell 10, moves in a second direction at least one of the sample cell 10 and an objective lens 52 while at least one of the sample cell 10 and the objective lens 52 is moved in a first direction, the second direction being different from the first direction, and takes, at a plurality of imaging positions, images of cells contained in the urine sample by means of an imaging unit 50.

The present application relates to a kind of small molecule probes and its preparation method and application, the chemical structure of the probe is as shown in the following formula: Wherein, R1 is 1-propyl sulfonic acid or 1-butyl sulfonic acid;R2 independently selected from trifluoromethyl or sulfur pentafluoride group, the substitution position of R2 is the ortho, para or meta of hydroxyl on benzene ring;Preparation method is by the condensation reaction of salicylaldehyde containing fluorine substituent group and indoline, directly introduce containing fluorine substituent group into the spiro-pyran molecule structure with pH response, prepared with 19 F MRI and fluorescence imaging pH-responsive bimodal imaging small molecule probe.Compared with the prior art, the small molecule probe of the present application exhibits significantly different 19 F NMR chemical shift and fluorescence intensity, high-resolution 19 F MRI imaging and fluorescence imaging, greatly improve the sensitivity and accuracy of imaging, has important application prospect in the field of biosensing, cell imaging, medical diagnosis etc.

The present application relates to a tunable annular diaphragm cell imaging system and a dye-free deep learning identification method, relating to the technical field of optical imaging and image processing, comprising an annular diaphragm illumination module; the present application establishes a quantitative mapping relationship between the outer diameter of the annular diaphragm parameter and the image quality index, and adaptively finds the best bright field and dark field illumination conditions for different cell types, densities and overall profiles and internal structures, so as to obtain an original image with sharp edges and distinct internal structures without using any dye, the improved deep learning segmentation model has a two-way attention gate module, which can capture spatial details and texture semantic information important for cell recognition in parallel and differentially, realize high-accuracy dye-free cell dead or alive automatic identification, make the segmentation result more consistent with the biophysical characteristics of cells, improve the segmentation accuracy and recognition accuracy, and automatically distinguish the state of cells with complex background without fluorescent labeling.

This invention belongs to the field of fluorescence analysis technology and relates to the application of a sulfur quantum dot fluorescent probe in tryptophan detection. This invention utilizes sulfur quantum dot probes to explore exogenous tryptophan cell imaging technology, studies the role of ultraviolet light in cell imaging, and enhances the fluorescence signal intensity of the sulfur quantum dot fluorescent probe by ultraviolet light irradiation, effectively achieving in vitro quantitative detection of tryptophan and highly sensitive, highly selective in situ detection in living cells. This invention can be used for the detection of tryptophan in vitro and in living cells, and has promising clinical application prospects in the biomedical field.

This application discloses a cell imaging device and workstation, relating to the field of biodetection technology, to alleviate the problem of operators accidentally inserting or removing samples during operation of cell imaging devices in related technologies. The cell imaging device includes a frame, a sample mounting device, a light source device, an imaging device, and a shielding component. The sample mounting device is mounted on the frame and includes a sample mounting port; the light source device is mounted on the frame and provides illumination light to the sample on the sample mounting device; the imaging device is mounted on the frame and located to one side of the sample mounting device; the shielding component is movably mounted on the frame; when the shielding component is in a first position, the sample can be mounted onto the sample mounting device through the sample mounting port; when the shielding component is in a second position, the shielding component blocks the sample mounting path corresponding to the sample mounting port. Therefore, this application has the advantages of improving the sample protection and application reliability of the cell imaging device.

A sample monitoring structure includes fluorescently-labeled hydrogel that is size-responsive to changes in one or more properties of aqueous liquid medium contacted with the sample monitoring structure. An intensity of a fluorescent emission from the sample monitoring structure changes with changes in the size of the fluorescently-labeled hydrogel. The sample monitoring structure is advantageously used in automated processing and analytical evaluation of large numbers of fluid samples over extended periods of time analytical evaluation systems, including flow cytometry systems, live cell imaging and analysis systems and parallel bioreactor system. The sample monitoring structure may be in a form of sample monitoring beads for addition to liquid media of fluid samples subjected to analytical evaluation.

1. Name of the product in this design: Intelligent Live Cell Imaging Analyzer. 2. Purpose of this design: This design is used for imaging observation and real-time image acquisition of live cells. 3. The key design features of this product are its top view. 4. The image or photograph that best illustrates the design's key features: a top view.

The present disclosure relates to imaging systems and methods of use thereof for imaging with simplified structured illumination microscopy (SIM). The present methods may be simpler and less costly to build and maintain that existing SIM systems. The imaging methods may include oscillating a portion of the imaging system to scan an illumination pattern across a field of view of the sample.

The present application relates to the technical field of live cell super-resolution optical imaging, and particularly relates to a live cell adaptive illumination imaging method and system. The method comprises the following steps: building a live cell adaptive illumination imaging system, including an illumination imaging module, an image sensing module, a decision control module and an imaging acquisition module; performing initialization and calibration; through image sensing feature extraction and photochemical kinetics model calculation, establishing an illumination-imaging-light damage coupling model, and extracting a seven-dimensional state vector of the cell; executing deep reinforcement learning reasoning, and outputting an optimal illumination control instruction; driving the high-speed stripe generation unit and the nanometer precision phase control unit in the illumination imaging module to work cooperatively, completing structured light illumination and image acquisition; performing super-resolution image reconstruction and iterative optimization output, and completing long-term low-light toxicity live cell imaging. The advantage lies in realizing real-time closed-loop feedback of illumination parameters, significantly improving photon utilization, reducing light toxicity, and improving response speed and long-term stable imaging.

The present disclosure provides a fluorescently-traceable amino acid derivative and a preparation method and use thereof, and belongs to the technical field of biomedicine. In the present disclosure, an amino acid skeleton of the amino acid derivative is modified mainly by a fluorescently-traceable functional group. 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) has low polarity and strong fluorescence, and can be used to modify an N-terminal of the amino acid skeleton to conduct subcellular imaging. Moreover, the NBD-Cl has a relatively small volume, lacks reaction orthogonality, and causes little interference with biochemical reactions of the organism itself. The results of examples show that the fluorescently-traceable amino acid derivative has a desirable biological activity and can be fluorescently traced in vivo and in vitro.

This invention belongs to the field of fluorescent carbon quantum dot technology, specifically relating to a dual-color, dual-targeting carbon quantum dot, its preparation method, and its applications. The carbon quantum dots used in this invention have abundant surface functional groups, making them easily modifiable. Ion modification can alter their surface functional groups, transforming them from a single-targeting method targeting mitochondria to a dual-color, dual-targeting method targeting both mitochondria and lysosomes, resulting in excellent cell imaging and enabling the observation of interactions between different organelles. The dual-color, dual-targeting carbon quantum dots provided by this invention eliminate the need for simultaneous staining with two fluorescent probes with good restaining properties, simplifying multi-probe staining operations and reducing factors affecting cells. The dual-color, dual-targeting method provided by this invention exhibits accurate localization, strong photostability, and maintains good stability before and after ion modification. The dual-color, dual-targeting carbon quantum dots provided by this invention have low toxicity to live cells, making them suitable for long-term live-cell imaging.

Anti-transthyretin (TTR) antibodies, corresponding polynucleotides, and expression vectors, as well as compositions (e.g., pharmaceutical compositions) containing anti-TTR antibodies as drugs and related manufactured articles are provided herein. Methods for treating or preventing transthyretin-mediated amyloidosis (ATTR) in subjects requiring such treatment or prevention using the pharmaceutical compositions described herein are also provided herein. Additionally, methods for verifying, identifying, and screening amyloid-depleting drugs using high-resolution live cell imaging are provided herein, as well as methods for producing pharmaceutical compositions of amyloid-depleting drugs and kits suitable for use in said methods.

This invention discloses a pH-responsive rosin-based Schiff base AIE surfactant, its preparation method, and its applications. The molecular structure of the pH-responsive rosin-based Schiff base AIE surfactant is as follows: Rosin is used as a raw material, and maleic amylopectin is obtained through D-A addition. Then, imidization and condensation reactions are performed to obtain the pH-responsive rosin-based Schiff base AIE surfactant. This surfactant exhibits good AIE performance and biocompatibility. In the solid state, this surfactant displays photochromic properties, while in aqueous solution, it exhibits good pH responsiveness. It can be used for cell imaging, anti-counterfeiting, and information encryption, expanding the range of rosin-based surfactants. This surfactant can also be used to prepare pH-responsive AIE supramolecular hydrogels. The hydrogels prepared from this surfactant possess excellent mechanical properties, temperature resistance, pH responsiveness, shear recovery, and fluorescence. Furthermore, they are injectable and malleable, and can be applied in anti-counterfeiting materials, biomimetic materials, tissue engineering, and other fields.

This document provides anti-thyroxine transporter (TTR) antibodies, corresponding polynucleotides and expression vectors, and compositions (e.g., pharmaceutical compositions) containing anti-TTR antibodies as drugs, and related articles. This document also provides methods for treating or preventing thyroxine transporter-mediated amyloidosis (ATTR) in subjects of need using pharmaceutical compositions. Additionally, this document provides methods for validating, identifying, and screening amyloid depletion drugs using high-resolution live-cell imaging, methods for generating pharmaceutical compositions of amyloid depletion drugs, and kits suitable for said methods.

Provided are methods and devices for assessing biological particles for use in cell immunotherapy. By utilizing a microfluidic chip device together with optical force measurement and cell imaging, the methods enable comprehensive assessment and characterization of biological particles with regard to morphology, motility, binding affinities, and susceptibility to external forces, including but not limited to, chemical, biochemical, biological, physical and temperature influences. The methods enable the selection and production of biological particles, such as engineered T-cells, for use in immunotherapy and biomanufacturing.

The present invention relates to a bioreactor for culturing samples and allowing simultaneous live-cell imaging of the samples, a gas exchange unit for allowing gas exchange of the culture medium during cell or tissue culture, thereby allowing the bioreactor to be kept suitable for long-term storage outside a culture chamber, a system comprising the bioreactor and the gas exchange unit, and a method for culturing samples.

A plasmonic nanostructure includes a dielectric substrate, a periodic array of dielectric pillars on the dielectric substrate; and, on each dielectric pillar of the periodic array, a respective conductive layer. Each dielectric pillar of the periodic array of dielectric pillars is between the respective conductive layer and the dielectric substrate. A method for imaging a cell includes reflecting an optical beam off the plasmonic nanostructure. The plasmonic nanostructure has biological cells adhered thereto. The method also includes collecting the reflected optical beam with an optical detector.

The present application relates to the technical field of cell imaging, and particularly relates to a method for observing the surface structure of the nucleus of a cell. The method comprises nuclear-cytoplasmic separation and observation of the nucleus. The nuclear-cytoplasmic separation comprises cell pretreatment and centrifugal separation of cytoplasm and nucleus. The cell pretreatment comprises placing the cell in a culture medium comprising nocodazole and cytochalasin B for 1.5-3 hours; the culture medium comprises, in terms of weight parts, 0.1-0.5 parts of nocodazole and 2-10 parts of cytochalasin B. The present application provides a nuclear-cytoplasmic separation method for treating cells with nocodazole and cytochalasin B, which can quickly obtain the nucleus while improving the problems of nucleus shrinkage and collapse, and completely preserving the structure of the nucleus, which has important value in the field of observing the surface ultrastructure of the nucleus.

The application discloses a sample analysis system, and relates to the technical field of sample analysis, and comprises the following steps: in response to a cell imaging graph query request, obtaining a target cell imaging graph to be queried according to the cell imaging graph query request, and displaying the target cell imaging graph, wherein the target cell imaging graph is a panoramic field of view graph or a segmented field of view graph; if an inputted segmentation instruction is received in the displayed target cell imaging graph, determining a corresponding target formed component according to the segmentation instruction; and updating the component type of the target formed component in the target cell imaging graph to a segmentation type specified by the segmentation instruction. The application realizes the support of segmentation when the cell imaging graph is queried, thereby improving the accuracy of the sample analysis result.