158 results about "Cellular imaging" patented technology

According to the cell fixing liquid, phenol oxidase is used for catalyzing phenol to be oxidized into a quinone structure, the quinone structure reacts with protein amido to form light covalent immobilization, cell immobilization is achieved, a stable hydration environment and membrane protection are provided through the ionic liquid solvent choline dihydrogen phosphate, a phosphate-free buffer system is provided, and cell immobilization is achieved. Therefore, a novel cell fixing liquid is obtained, the cell structure integrity required by cell imaging analysis is effectively reserved, meanwhile, cell staining fluorescence signal interference is avoided, and therefore the accuracy of cell counting and cell imaging analysis is ensured.

Devices, systems, and methods are provided for in vivo fluorescence imaging. Disclosed herein is an implantable miniature fluorescence imager on a chip having a custom imaging array with angle selective gratings, fiber optics, or microcollimators for image deblurring, and optical filters that can be tuned to image fluorescence from multiple fluorophores simultaneously. Power is supplied by an on-chip power source or transmitted to the chip from an external transducer such as an ultrasound transducer, electromagnetic transducer, inductive transducer, or radiofrequency transducer. Wireless communication may be provided by electromagnetic or ultrasound links to the device. The function of a fluorescence microscope is provided in a millimeter-scale device that can be readily implanted in tissue and used to image fluorescently labeled cells in vivo. The small size of the fluorescence imager makes possible sustained in vivo imaging with real-time monitoring of multiple cell types within Shifting the dynamic diseased tissue or a tumor.

The invention discloses a vesicle transportation path prediction system based on artificial intelligence, and relates to the technical field of vesicle transportation, which comprises the following steps of: accurately extracting a complete time sequence movement track of a single vesicle before and after disturbance by virtue of fluorescence living cell imaging under a disturbance experiment condition in combination with a target detection model YOLOv8 and a depth correlation tracking algorithm DeepSORT; the problem that perturbation conditions are insufficiently perceived is solved, through a multi-modal data matrix, the model has the time sequence perceiving capacity for changes of a local space structure where vesicles are located, so that the sensitivity and modeling capacity of the model for track variation in a complex environment are remarkably improved, deep fusion of multi-modal features is achieved by building a double-branch fusion model, and the accuracy of the model is improved. The problem that an existing prediction model cannot actively recognize and correct prediction deviation is effectively solved, and the stability and the actual usability of the system are improved.

According to the invention, a collaborative deformable mirror, a two-dimensional galvanometer scanning system and a high-precision three-dimensional displacement sample table are designed, and rapid tracking and spot scanning imaging of a freely moving sample are completed. And under the condition of single cell analysis resolution, the tracking range is large, and the imaging speed is high. As the galvanometer controls the deflection angle through the driving voltage, the propagation path of the imaging light path can be efficiently adjusted in real time. Therefore, the tracking response speed based on the galvanometer scanning system is obviously superior to that of a traditional mechanical displacement compensation mode. The displacement is compensated through the sample table, and the tracking range is expanded. The displacement compensation process adopts a sample motion trail pre-judgment algorithm, is carried out in real time and is small in compensation amount, and rapid response of the system is ensured; displacement compensation and galvanometer tracking work cooperatively, the tracking speed is optimized, and spot scanning imaging is completed at the same time. Axial scanning is completed by modulating excitation light wave front through a deformable mirror. The system is integrated with a light stimulation module, wavefront phase modulation is carried out on stimulation light by utilizing a spatial light modulator, single-point or multi-point focusing is realized, single or multiple neurons can be accurately controlled, and the system has important significance on research of neuroscience.

A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

The invention discloses a thiophene site specific modified TAE material as well as a preparation method and application thereof, and relates to the technical field of organic optical functional materials. A main body structure of the TAE material specifically modified by thiophene sites is tetraarylethene, thiophene substitution exists on the tetraarylethene, and R groups are introduced to the tail end of thiophene, or the TAE material specifically modified by the thiophene sites is obtained. The invention also provides an application of the TAE material or the photochemical sensor specifically modified by the thiophene site in photochromism or mechanochromism or cell imaging or safe ink or anti-counterfeiting. According to the invention, through conjugate coupling of a thiophene ring and an ethylene bridged benzene ring, a diarylethene functional unit with light-induced isomerization capability is formed, and the aggregation-induced emission (AIE) performance of the material is remarkably improved while the light response activity is realized. The synergistic effect endows the material with revolutionary potential in the fields of light-operated bimodal patterning technology and multi-dimensional anti-counterfeiting application.

The invention discloses a difunctional near-infrared fluorescent probe as well as a synthesis method and application thereof. The structural formula of the fluorescent probe is shown as a formula (I). The fluorescent probe DTPD provided by the invention can be used for fluorescent quantitative detection of hypochlorous acid and viscosity, and the fluorescent probe can realize detection of hypochlorous acid with high selectivity in an aqueous solution and is not interfered by other coexisting substances. Besides, the near-infrared fluorescent probe provided by the invention has relatively strong organism penetrability in a near-infrared region, can also reduce the interference of autofluorescence of organisms, and has a good cell imaging effect. And # imgabs0 #.

The invention relates to the technical field of cell manipulation and analysis, and relates to an automatic single cell continuous capturing and screening system and a use method thereof. Comprising a bias liquid storage module, a cell injection module, a micro-fluidic chip, a cell capturing module, a cell screening module and an imaging system, the bias liquid storage module, the cell injection module, the cell screening module and the cell capturing module are connected with the micro-fluidic chip; the bias liquid storage module is used for generating bias flow; the cell injection module is used for providing cells; the micro-fluidic chip is used for capturing and screening cells; the cell capturing module is used for capturing cells; the cell screening module is used for screening cells; and the imaging system is used for acquiring captured cell images and performing visual analysis. Micro-fluidic pipeline double-bias-flow stable cell flow sample introduction is designed, a high-speed electromagnetic valve is matched to realize rapid control of a gas path on cell capturing and screening channel fluid, and a high-speed camera is combined to analyze cell positions in different areas in real time, so that automatic single cell continuous capturing and screening are realized.

The present disclosure is generally directed to anchoring bridge nucleic acid probes in a polymer matrix, which are configured to hybridize to cellular nucleic acid, embedding in a polymer matrix, clearing cellular components including the cellular nucleic acid, contacting the anchored bridge probes with primary probes that hybridize with the bridge probes and imaging. The disclosure describes methods for imaging cellular RNA in an RNase insensitive method.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

Methods and processes disclosed herein generally involve training a machine learning model to identify a condition active in changing a cellular phenotype. In particular, cells having the cellular phenotype can be cultured in wells having a reference condition that changes the cellular phenotype, along with a background compound to provide a reference plurality, and cells having the cellular phenotype can be cultured in wells having a background compound to provide a non-reference plurality. A staining and imaging assay can be performed on the reference and non-reference pluralities after incubation, and the images are applied to a machine learning model to classify the results into activity data, to identify a "hit" activity plurality as corresponding to the reference plurality, and a "non-hit" activity plurality as corresponding to the non-reference plurality. The machine learning model can be used in a process to identify a test condition predicted to be active in changing a cell line phenotype.

Facet-based DNA polyhedral nanostructures with single strands of DNA for each polyhedron face. The DNA-based polyhedral nanostructures may be configured for targeted drug delivery and cell imaging for therapeutic, diagnostic and analytical diagnostic applications. The DNA polyhedral nanostructures adopt platonic icosahedral and dodecahedral configurations resembling the natural shapes of viral nucleocapsid proteins. The DNA polyhedral nanostructures address multiple technical challenges in the biomedical and biotechnological fields, including reducing the cost and complexity associated with conventional DNA origami systems, enhancing the sensitivity of diagnostic platforms, improving tracer delivery and signal clarity in PET and MRI, providing structural stability for nanostructure and payload integrity, and improving the resolution of Cryo-EM imaging of small molecules.

1. Name of the product in this design: Integrated instrument for in situ cell culture and dynamic imaging. 2. Purpose of this design: for in situ cell culture, and to simultaneously perform real-time dynamic imaging of cells from the bottom. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention belongs to the technical field of cytobiology and optical imaging, and particularly relates to a protein point recognition and analysis method and device based on real-time cell imaging. The protein point recognition method based on real-time cell imaging comprises the following steps: S11, acquiring a cell fluorescence image, performing denoising processing on the cell fluorescence image by adopting a non-local mean denoising algorithm, and removing background nonuniformity by using morphological opening operation to obtain a preprocessed image; and S12, dynamically calculating a threshold value of the preprocessed image based on local image intensity, carrying out binaryzation to obtain an image with a background and a foreground, carrying out morphological operation on the image to remove noise and artifacts, and carrying out image region segmentation on the image to obtain an image with a plurality of protein points. According to the method, a non-local mean denoising algorithm and morphological background correction are combined, so that the quality of the low-signal-to-noise-ratio cell fluorescence image is remarkably improved.

The present invention provides a D-D-π-A type organic photosensitizer and a synthesis method and application thereof, belonging to the field of photosensitizer and photodynamic therapy application of tumors. The present invention uses triphenylamine and carbazole as electron donors, malononitrile as an electron acceptor, and thiophene as an electron π bridge to synthesize an AIE type photosensitizer with mitochondrial targeting function through a series of simple organic reactions. The AIE type photosensitizer can be used in the synergistic photodynamic and ferroptosis treatment of tumors. The photosensitizer can realize tumor cell imaging and target mitochondria, and can selectively exert photodynamic and ferroptosis effects on tumor cells. It has good therapeutic effect and biosafety, and has strong application prospects in non-invasive tumor treatment.

The application discloses a cell cutting method and device, electronic equipment and computer program product, wherein the method comprises: acquiring a plurality of first files; each of the plurality of first files is used for a cell cutting system to cut a group of cells in a plurality of groups of cells imaged in a first image; the group of cells comprises one or more cells; the first image is determined based on an image output after a cell imaging system performs cell imaging processing on a target tissue; based on the plurality of first files, a second file is generated; the second file contains first data for describing a cutting path of each cell in a plurality of groups of cells corresponding to the plurality of first files; and the second file is output to the cell cutting system, so that the cell cutting system cuts a plurality of groups of cells imaged in the first image based on the second file.

This invention relates to the field of organic small molecule fluorescent probe technology, specifically to a neuron-specific dual-targeting fluorescent probe targeting the AChR of cholinergic neurons and its applications. The fluorescent probe, whose structure follows general formula I, is characterized by its dual-targeting structure, making it particularly suitable for the identification and labeling of cholinergic neurons. Furthermore, this fluorescent probe exhibits significant advantages in multiple application scenarios, such as cell imaging, protein and organelle labeling, tumor-specific recognition, and photodynamic therapy, with its potential application value in neuroimaging being particularly prominent. This fluorescent probe is expected to advance the development of neurobiology, disease diagnosis, and treatment methods, and has promising application prospects.

The invention discloses a cell cutting method and device, electronic equipment and a computer program product, and the method comprises the steps: obtaining a plurality of first files; each first file in the plurality of first files is used for the cell cutting system to cut one group of cells in a plurality of groups of cells imaged in the first image; the group of cells comprises one or more cells; the first image is determined based on an image output after a cell imaging system performs cell imaging processing on a target tissue; generating a second file based on the plurality of first files; the second file comprises first data used for describing a cutting path of each cell in a plurality of groups of cells corresponding to the plurality of first files; and outputting the second file to a cell cutting system, so that the cell cutting system cuts the plurality of groups of cells imaged in the first image based on the second file.

The invention discloses a targeted KRAS G12C environment-sensitive fluorescent probe as well as a preparation method and application thereof, the fluorescent probe is a compound shown in the following formula or pharmaceutically acceptable salt thereof, and the structural formula is shown in the specification. The invention also specifically discloses a preparation process of the targeted KRAS G12C environment-sensitive fluorescent probe and application of the targeted KRAS G12C environment-sensitive fluorescent probe in preparation of a living cell imaging detection preparation. According to the invention, a known KRAS G12C covalent inhibitor structure is reasonably modified according to a molecular docking mode of the inhibitor and KRAS G12C, and an environment-sensitive fluorescent labeling group is introduced to form the environment-sensitive fluorescent probe with a switch mechanism, so that the environment-sensitive fluorescent probe has the advantages of simplicity and convenience in operation, no need of washing, no need of paraformaldehyde fixation of cells and the like; the invention aims to realize the intuitive visualization of KRAS G12C in living cells / in vivo, and assists in the research and visual application of targeted therapy.

The application discloses a phosphorescent on-off type biological orthogonal iridium complex probe and a preparation and application thereof, and belongs to the technical field of organic photoelectric materials. A benz[h]quinoline-5,6-dione derivative is used as a C^N ligand, the C^N ligand is subjected to a coordination reaction with an iridium trichloride trihydrate to obtain a cyclometalated iridium chloro-bridged dimer, and then the cyclometalated iridium chloro-bridged dimer is subjected to a reaction with an N^N ligand to obtain the iridium complex probe. After the probe is reacted with a biological orthogonal unit, angelica lactone, the excited state energy level of the complex is changed, the photophysical property of the complex is influenced, the phosphorescence is turned on from nothing, and the effect of phosphorescence on-off is achieved. The phosphorescence on-off characteristic makes the probe before and after the reaction with the angelica lactone present obvious luminescent signal ratios. The characteristic can maximally reduce background signal interference, is convenient for cell imaging and biomolecular recognition, and can provide a new train of thought for developing a novel phosphorescent on-off type biological orthogonal material. The application has a good application prospect in the field of biological marking and imaging.

The invention discloses an anti-aging three-dimensional high-uniformity dermis evaluation model based on image analysis, and belongs to the technical field of biological medicine and cosmetic efficacy evaluation. The method comprises the following steps: mixing a prepared fibroblast suspension and a neutralized collagen solution at 0-5 DEG C, inoculating the mixture into a 24-pore plate in a 100-200L / pore manner, standing in an incubator at 37 DEG C for 20-40 minutes to form collagen gel, carrying out suspension culture to obtain a 3D dermal model, and detecting the shrinkage rate of the model and evaluating the anti-aging effect in combination with a high-content cell imaging system. The method overcomes the defect that a traditional method is large in error, an efficient and accurate evaluation system is provided for anti-aging drug screening, and the method has application value in cosmetic effect detection and skin aging mechanism research.

The invention provides a cell imaging adjusting device and a cell imaging device.The cell imaging adjusting device comprises a focusing assembly, a collecting assembly and a light source assembly, the collecting assembly is used for shooting cell imaging in a sample to be detected, and the collecting assembly comprises a collecting body and a lens installed on the collecting body; the connecting piece is connected between the acquisition body and the focusing assembly; the light source assembly is opposite to one end of the lens away from the connecting piece; a mounting space for mounting a to-be-tested sample is arranged between the light source assembly and the lens; and the focusing assembly is used for driving the acquisition assembly to move. The focusing assembly drives the lens of the collecting assembly to be far away from or close to the sample to be detected, focusing operation during cell imaging counting is completed according to needs, and the cell imaging counting device is simple in structure, small in size, low in cost and easy and convenient to operate.

1. The name of the design product: cell imaging analyzer. 2. The use of the design product: for imaging analysis of cells. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The present invention discloses a three-dimensional laser post-ionization-secondary neutron particle mass spectrometer, which includes a vacuum chamber, a sample introduction system, a primary ion emission system, a three-dimensional laser post-ionization system, an ion optical system, a time-of-flight mass spectrometer, an observation system and a computer control system. By using a tunable laser, the present invention can achieve selective ionization of substances by adjusting the wavelength, frequency and energy value of the laser to make the energy of the laser photons resonate with the electronic energy level of the target molecule. This method has extremely high element, nuclide and molecular selectivity, can provide the sensitivity of the instrument, and can be applied to single-cell imaging analysis and testing of lipids, metabolites, small molecule drugs with molecular weights less than 1000 Da and some elements, solve the problems of small molecule metabolite testing in spatial metabolomics and ultra-high spatial resolution imaging. When not using laser post-ionization, it can be used alone as SIMS.

The invention provides a single-component boron-nitrogen-doped dual-emission carbon quantum dot and a preparation method and application thereof.The carbon quantum dot is synthesized through a triaminophenylboronic acid one-step hydrothermal method, 370 nm and 420 nm dual-emission peaks are generated at the same time under 320 nm excitation, the average particle size ranges from 2.0 nm to 2.3 nm, the boron doping amount ranges from 8% to 12%, and the nitrogen doping amount ranges from 2.5% to 4.0%. The carbon quantum dot as a ratiometric fluorescent probe can simultaneously detect Fe < 3 + > and Hg < 2 + >, has detection limits of 0.069 [mu] M and 0.1524 [mu] M respectively, has linear response in a pH range of 8.0-13.0, and is successfully used for cell imaging. The problems that a traditional multi-component probe is complex in preparation and poor in stability are solved, multifunctional integrated detection is achieved, and the probe has wide application prospects in the fields of environmental monitoring and biomedicine.

This invention discloses a method for studying the mechanism by which the mucoprotein channel 1 (TRPML1) regulates the outflow of zinc ions from lysosomes and induces changes in mitochondrial ATP. This method uses 13 nm AuNPs as loading materials to construct a logic-gated sensor for pH and zinc ion regulation within lysosomes and for TRPML1 mRNA in the cytoplasm. It also uses 5 nm AuNPs as loading materials to construct a sensor targeting mitochondrial ATP for cell imaging. This method enables the detection of mRNA and zinc ions in the cytoplasm, lysosomes, and mitochondria. 2+ ATP-associated ATP sensors, Zn 2+ Three sensors—a sensor, an mRNA sensor, and an X-ray sensor—are used simultaneously to image cells, aiming to answer questions about the mechanisms of ATP changes in mitochondria under the regulation of zinc ion efflux from lysosomes by TRPML1.

The application provides a novel ratio near-infrared fluorescent probe for detecting sulfur dioxide, a preparation method and application of the fluorescent probe, the fluorescent probe is Cou-Oxo, the preparation method comprises the following steps: dissolving a compound Cou-CN and a compound 3 in toluene, adding piperidine to catalyze a reaction, refluxing at 114-116 DEG C under a nitrogen atmosphere for 4.8-5.2 hours, and after the reaction is completed, the fluorescent probe Cou-Oxo is obtained through purification.The ratio fluorescent probe has good light emitting characteristics, can effectively detect sulfur dioxide derivatives, has the potential of quantitative detection, and the lower limit of detection is 0.942 muM.The ratio fluorescent probe has a fast response to the detection of sulfur dioxide derivatives, has good light emitting characteristics and stability, and can detect HSO3 ‑ in a physiological pH range of 7-9; the ratio fluorescent probe has lower biological toxicity and is suitable for cell imaging.

This invention provides a benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe for lipid droplets and lysosomes, its preparation method, and its application, relating to the field of fluorescent probes. The preparation method of the benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe includes the following steps: a primary reaction and a secondary reaction. The benzothiadiazole-based dual-target viscosity-sensitive fluorescent probe of this invention can simultaneously target and monitor two organelles (lipid droplets and lysosomes), and exhibits low cytotoxicity and accurate cell localization in HeLa cell imaging experiments; furthermore, it achieves both good polarity response and effective response to intracellular viscosity, demonstrating excellent response performance.

Provided are a cell imaging device and a cell imaging method that can shorten the time period of taking images of cells in a liquid sample, compared with conventional techniques. This cell imaging device 100 introduces a urine sample containing cells into an internal space 11 of a sample cell 10, moves in a second direction at least one of the sample cell 10 and an objective lens 52 while at least one of the sample cell 10 and the objective lens 52 is moved in a first direction, the second direction being different from the first direction, and takes, at a plurality of imaging positions, images of cells contained in the urine sample by means of an imaging unit 50.

The invention discloses an automatic focusing module and an automatic focusing method for high-flux living cell imaging. The automatic focusing module comprises a base; a Z-axis moving mechanism is arranged on the base; a converter fixing seat is arranged on the Z-axis moving mechanism; an electric converter is arranged on the converter fixing seat; the electric converter is provided with a laser automatic focusing sensor and an objective lens. The laser automatic focusing sensor and the objective lens are integrated on the electric converter, when the laser automatic focusing sensor detects the distance of a specimen, the laser automatic focusing sensor does not need to move along the X-axis and the Y-axis, the position of the objective lens only needs to be switched through the electric converter, the Z-axis moving mechanism drives the objective lens to move to a proper position along the Z-axis, and then photographing and imaging can be completed. Errors caused by repeated displacement can be effectively avoided, the imaging effect is improved, the working efficiency can be improved, and the working time can be shortened.