Construction and application of gold-magnetic nanoparticle-based medicament carrying platform
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A gold nanoparticle and magnetic nanoparticle technology, applied in the field of nanomaterials, can solve the problems of inability to exert anti-cancer effects, poor biocompatibility, low solubility, etc., and achieve good chemical inertness, biocompatibility and easy function Chemical, strong magnetic effect
Inactive Publication Date: 2010-06-02
JIANGNAN UNIV
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[0007] The current technical problem that needs to be solved urgently is to provide a biocompatible surface biofunctionalized core-shell nano-platform and its preparation method and application,
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Embodiment 1
[0055] (1) Co-precipitation method to synthesize magnetic nanoparticles
[0056] ① Weigh Fe separately 3+ , Fe 2+ Soluble in 80mL ultrapure water, Fe 3+ and Fe 2+ The total concentration of Fe is 0.08mol / L, Fe 3+ : Fe 2+ Molar ratio 1-1.2:2, 80°C, 400r / min stirring reaction for 10min;
[0057] ② Add 5mL of ammonia water drop by drop, and keep the pH at 10.0, and react for 30 minutes after the dropwise addition;
[0058] ③ Ultrasonic mixing, washing 2 to 3 times;
[0059] ④Magnetically separate the dry powder to obtain magnetic nanoparticles.
[0060] (2) SiO 2 Encapsulated Magnetic Nanoparticles
[0061] Using the inverse microemulsion method, in the water-in-oil environment, dissolve tetraethyl orthosilicate into silicon balls, and wrap the magnetic nanoparticles.
[0062] ①Add triraton and 7 mL of n-hexanol, mix and sonicate for about 20 minutes until oil-free filamentous liquid;
[0063] ②Sonicate 28mL cyclohexane for about 45min, pour it into a 100mL three-necke...
Embodiment 2
[0085] (1) Cell culture
[0086] After resuscitating the leukemia cells HL-60, in IMDM medium containing 20% fetal bovine serum, 37°C, 5% CO 2 The environment was cultivated until the cells entered the exponential growth phase (logarithmic phase).
[0087] (2) Characterization of multifunctional nanoplatforms
[0088] ①Magnetic Resonance Imaging (MRI) Characterization of Phagocytosis Multifunctional Nanoplatform
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Abstract
The invention relates to the construction and application of a gold-magnetic nanoparticle-based medicament carrying platform, which belong to the technical field of magnetic nanophase materials. The construction comprises the synthesis of magnetic nanoparticles, the synthesis of an Fe3O4-SiO2 nuclear shell structure, the amine surface modification of the Fe3O4-SiO2 nuclear shell structure, protonation and the electrostatic adherence of gold nanoparticles after the surface modification, the coupling of curcumin and the coupling of mercapto polyethylene glycol PEG-SH so as to prepare a multifunctional gold-magnetic nano-platform. The construction and the application have the following advantages that: the gold-magnetic nanoparticle-based medicament carrying platform is a magnetic carrier with a modified surface and has the characteristics of relatively stronger magnetism, good chemical inertness, biocompatibility, easy functionalization and the like, and can be prepared by a simple method; and the magnetic nanophase materials can be quickly separated with a magnetic field in a suspension system, are highly concentrated and act on tumor cells under the guidance of an external magnetic field, and can be applied in biological fields, such as bioseparation, medicament transportation, biological tags, cell imaging and developing and study on the separation of cells or other biomolecules.
Description
technical field [0001] The invention relates to the construction and application of a drug-loading platform based on gold magnetic nanoparticles. The invention belongs to the field of nanomaterials, and more specifically relates to nanomaterials used in drug delivery and tumor treatment in life sciences. Background technique [0002] Biofunctional materials are a hot field in materials science research. Since the 1980s, nanomaterials have attracted more attention from scientists due to their special physical and chemical properties, so that nanotechnology can be used to study and solve major problems in the field of biology. Problems have also become one of the frontier research areas. [0003] Due to its unique properties (no remanence), superparamagnetic nanoparticles have broad application prospects in biomedical fields such as bioseparation, immunoassay, and targeted drugs. However, its own chemical stability, colloidal stability, and poor biocompatibility are disadvant...
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