355 results about "Immunoassay" patented technology

The invention relates to a hybridoma cell strain capable of secreting a mefos monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. According to the invention, the hybridoma cell strain capable of secreting the fenofos monoclonal antibody is obtained through screening, the fenofos monoclonal antibody has high specificity and high sensitivity to the fenofos, IC50 to the fenofos is 0.231 ng / mL, and the fenofos monoclonal antibody has no cross reaction to structural analogues of the fenofos, such as triazophos, diazinon, phorate, acetyl phorate, methamidophos and the like; therefore, low-concentration isoprophos can be accurately detected.

The invention relates to a hybridoma cell strain capable of secreting dextromethorphan monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The monoclonal antibody secreted by the hybridoma cell strain disclosed by the invention has good sensitivity and specificity on dextromethorphan, wherein the IC50 value on the dextromethorphan is 0.162 ng / mL. The monoclonal antibody provided by the invention has no cross reaction on structural analogues of dextromethorphan, such as fentanyl, thilazine, papaverine, morphine and medetomidine, and has good specificity, so that the dextromethorphan with low concentration can be accurately detected.

The invention discloses a preparation method of Fe3O4 magnetic nanoparticles rich in carboxyl groups on the surface, which comprises the following steps: modifying Fe3O4 nanoparticles by using polyethylene glycol and silicon dioxide to enhance the dispersity and biocompatibility of the magnetic nanoparticles, modifying the surfaces of PEG-Fe3O4 coated SiO2 nanoparticles by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface, and modifying the magnetic nanoparticles rich in carboxyl groups on the surface by using a silane coupling agent containing silane groups and unsaturated double bonds to obtain the magnetic nanoparticles rich in carboxyl groups on the surface. Then octavinyl-POSS and double bonds on the surfaces of the nano particles are subjected to click modification to form an octahedral cage, 4-mercaptobenzoic acid and double bonds are further subjected to click modification, the surfaces of the nano particles are rich in carboxyl, and a polymer brush rich in carboxyl is constructed. Compared with a conventional streptavidin coupling method, a high-density polymer chain provides a large number of carboxyl active binding sites, so that the streptavidin coupling amount is increased, the antibody coupling rate is increased, and a solution is provided for applications such as high-sensitivity biological detection, efficient targeted drug delivery and stable immunoassay.

The invention discloses a biochemical immunodetection device and a biochemical immunodetection method for ursodesoxycholic acid reduzate, and belongs to the technical field of ursodesoxycholic acid reduzate detection. The ursodesoxycholic acid reduzate detection device comprises a detection box and a lifter at the bottom of the detection box, a dissolution preparation part and a centrifugal part are arranged in the detection box, and the dissolution preparation part can fully dissolve an ursodesoxycholic acid reduzate-containing medicament; the centrifugal part is used for carrying out precipitation separation on the dissolved medicament; the detector can accurately collect fluorescence signals of the immune complex; according to the ursodesoxycholic acid reduzate detection device, the integrated operation of detection test tube fixing, cover opening and transferring, medicament adding, cover closing and plug sealing and dissolving is achieved, the steps of automatic medicament filling, sealing and the like are achieved, the medicament preparation efficiency is greatly improved, efficient and accurate detection of ursodesoxycholic acid reduzate is further achieved, and the detection efficiency is improved. And complex and tedious manual operation is avoided, and the situation that the sample is easily polluted by manual operation is avoided.

The invention belongs to the field of immunodetection kits, and provides a P-Tau231 single-molecule immunodetection kit based on signal amplification and application of the P-Tau231 single-molecule immunodetection kit. The kit comprises a solid-phase carrier, a detection antibody-signal amplification probe conjugate and the like, wherein a total Tau non-phosphorylated region specific first antibody is fixed on the surface of the solid-phase carrier; the detection antibody-signal amplification probe conjugate is used for recognizing P-Tau231 phosphorylated 231 sites; quantitative detection is realized by adopting a sandwich immune sandwich mode and combining with a single-molecule detection platform. The detection limit of the kit reaches fg / mL to ag / mL, and the kit has the advantages of ultrahigh sensitivity, high specificity, simplicity and convenience in operation and low sample demand, can efficiently detect P-Tau231 in cerebrospinal fluid and plasma, and is suitable for preparation of early diagnosis products for Alzheimer's disease.

This invention belongs to the field of microcystin-LR detection technology, providing anti-microcystin-LR nanobodies and their applications. The anti-microcystin-LR nanobodies have amino acid sequences as shown in SEQ ID NO.1, SEQ ID NO.2, or SEQ ID NO.3. This invention uses phage display technology to screen for nanobodies with high affinity and high specificity for recognizing microcystin-LR, exhibiting advantages such as strong stability and ease of large-scale expression. Various detection tools can be designed based on anti-microcystin-LR, including test strips, immunoassay kits, immunoaffinity columns, biosensor detection chips, or detection probes. This provides novel antibody resources for rapid monitoring of microcystin-LR and lays a material foundation for developing low-cost, high-performance immunoassay technologies, which is of great significance for promoting the monitoring and control of microcystin-LR pollution.

To provide a method for solving problems related to the delivery of R2 polyhapten reagents, such as short delivery times or bubble collapse. [Solution] A novel and improved method for separating absorbance caused solely by the addition of assay reagents, and a novel and improved method for detecting and flagging problems, particularly those caused by the delivery of R2 polyhapten reagents, as well as apparatus and compositions for use herein, are provided.

A metal-resin composite that has a structure in which a plurality of metal particles are fixed to resin particles and, in the range of pH 3 to pH 10, the maximum value of the zeta potential is at least 5 mV and the minimum value is −5 mV or less. In this metal-resin composite, it is preferable that, in the range of pH 3 to pH 10, the difference between the maximum value and the minimum value of the zeta potential is at least 20 mV; and it is further preferable that the point of zero charge of the zeta potential is in the range of pH 3.5 to pH 9.0. This metal-resin composite can be used in immunoassays and in immunoassay reagents as a labeled substance by making antigens or antibodies adhere to the surface of the metal-resin composite.

This invention discloses a homogeneous microsphere based on singlet oxygen self-circulating aggregation-induced emission, its preparation, and a wash-free detection method, which improves the sensitivity and efficiency of wash-free luminescent oxygen channel immunoassay. This invention successfully prepared AIE receptor microspheres by embedding the photosensitizer AIER dye, ultra-bright AIEG dye, and singlet oxygen-responsive dye SO into carboxyl-modified polystyrene nanoparticles. Simultaneously, an antibody-1-labeled AIE receptor microsphere solution, an antigen-containing test solution, and biotinylated antibody-2 were added to a 96-well plate, followed by streptavidin-modified donor microspheres. After wash-free incubation, the fluorescence signal value was measured, and the concentration of antigen in the test solution was obtained according to a standard curve. This platform completes wash-free, highly sensitive detection within 5 minutes, innovatively combining the aggregation-induced emission characteristics of AIEgens and a singlet oxygen cycling strategy, providing an efficient and universal solution for wash-free diagnostics.

The application relates to a sensor for synchronously detecting enniatin and roquefortine based on a double-potential electrochemiluminescence immunoassay technology as well as a preparation method and a detection method thereof. The sensor comprises a base electrode, a sensing interface composed of a nanocomposite is modified on the surface of the base electrode, mixed coated antigens including ENNs-BSA and AOH-BSA fixed on the sensing interface, mixed probes including a cathode probe and an anode probe, the cathode probe is Zr-Por-MOF-ENNs Ab which is obtained by marking ENNs antibodies with a porphyrin zirconium metal organic framework, the anode probe is Ce-In-MOF@ABEI-AOH Ab which is obtained by marking AOH antibodies with ABEI loaded on a cerium-doped indium metal organic framework, and a detection solution containing a single co-reactant potassium persulfate. The application can realize one-time sample adding, double-potential scanning and synchronous quantification for two kinds of mycotoxins of enniatin and roquefortine.

This invention relates to a hybridoma cell line that secretes a Cy3.5 monoclonal antibody and its application, belonging to the field of immunoassay. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46741. The Cy3.5 monoclonal antibody secreted by this cell line exhibits excellent affinity and sensitivity to Cy3.5, with an IC50 of [missing value]. 50 Reaching 10 ng / mL, the IC50 for Cy5.5 50 An immunoassay product based on this hybridoma cell line, with a concentration of up to 50 ng / mL, can be used for the detection of Cy3.5 and its analogues, providing an efficient detection method and means for the residual detection of Cy3.5.

The invention belongs to the technical field of chemiluminescence immunoassay, and particularly relates to application of a luminescence retarder in improving development quality of solid-phase membrane immunodetection. According to the invention, when the concentration of the delay agent is fixed, the time of luminescence starting delay and the concentration of the enzyme catalyst are in nonlinear negative correlation, that is, the delay time is obviously increased along with the reduction of the enzyme concentration. The nonlinear negative correlation is suitable for luminescent systems of different reinforcing agents. Benefited from the enzyme concentration dependent luminescence delay effect, the chemiluminescence liquid with the delay effect is used for improving the development quality of solid-phase membrane immunodetection for the first time, noise and background signals are suppressed, and the chemiluminescence liquid has wide application value in the aspect of solid-phase membrane immunodetection.

This application provides an immunoassay analyzer, a magnetic separation device, and a method for cleaning the aspiration needle, belonging to the technical field of immunoassay equipment. The immunoassay analyzer includes a magnetic separation device, which includes a magnetic separation disk, a liquid injection device, an aspiration needle, and a controller. It also includes a separation disk heating module, or a separation disk heating module and a cleaning agent heating module. The magnetic separation device has a first mode and a second mode. In the first mode, it can clean the magnetic particles in the reaction cup. In the second mode, the controller can control the heating module to heat the aspiration needle cleaning agent to a first temperature. The aspiration needle cleaning agent has high activity and cleaning ability at the first temperature, and can efficiently clean the deposits attached to the inner wall of the aspiration needle.

In alternative embodiments, the invention provides vaccines, pharmaceutical compounds and formulations for diagnosing, preventing, treating or ameliorating Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections. In alternative embodiments, the invention provides compositions such as diagnostic tests, assays, immunoassays and test strips, and methods, for detecting or diagnosing the presence of a Streptococcal infection, e.g., Group A Streptococcus (GAS), Group C Streptococcus (GCS), or Group A Streptococcus (GGS), infections, or other pathogenic Streptococcus infections.

This invention relates to a hybridoma cell line secreting a monoclonal antibody against pendimethalin and its application, belonging to the field of immunoassay. The hybridoma cell line secreting the pendimethalin monoclonal antibody of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46744. The pendimethalin monoclonal antibody secreted by this hybridoma cell line is used for the analysis and detection of pendimethalin residues in food safety testing. The pendimethalin monoclonal antibody cell line obtained by this invention can be used for immunoassay detection, exhibiting good detection sensitivity and specificity (IC50) for pendimethalin. 50 The value was 1.87 ng / mL.

The application discloses a kind of areca alkaloid artificial hapten, artificial antigen and its preparation method and application, with norarecoline hydrochloride as the starting material for synthesizing artificial hapten.This areca alkaloid artificial hapten retains the characteristic structure of areca alkaloid to the greatest extent, and has active groups that can couple with carrier proteins, which can be used as antigenic determinants;Further, the areca alkaloid artificial antigen obtained can be used to immunize to obtain an anti-areca alkaloid artificial antibody with high affinity, high sensitivity and strong specificity.The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, and can be used for rapid and accurate immunodetection and immunoassay of areca alkaloid.

Described herein are methods and compositions for accurate detection of cancer using ultrasensitive immunoassays, e.g., digital ELISA, to detect open reading frame 1 protein (ORF1p), which is encoded by the LINE-1 retrotransposon, in biofluids.

This utility model relates to the field of immunoassay technology and discloses a shake-resistant fluorescent immunochromatographic reagent kit, including a placement box and a detection box. The detection box contains a test strip, a sample pad, and an absorption pad. The top of the detection box has a sample dispensing port and a display area. A support base is installed at the center of the top of the placement box, and a limiting seat is installed at the inner edge of the placement box. Both the top of the support base and the limiting seat have six sets of limiting grooves. This shake-resistant fluorescent immunochromatographic reagent kit can be stably placed using the placement box, and the test reagent can be tightly embedded in the limiting grooves for fixation. When adding the fluorescent immunochromatographic solution, excess liquid can fall into the placement box for collection and prevent leakage. The anti-slip pad at the bottom of the placement box improves stability, and the detection box embedded inside prevents shaking, thus achieving stable detection and solving the problem of poor stability.

This invention belongs to the field of immunoassay and biosensing technology, and discloses a method for preparing a ratiometric ECL biosensor based on Eu / Tb-Lu MOGs. Using Eu / Tb-Lu MOGs as the luminescent material and H3-tctpy and luminol as ligands, a single-luminescent material with dual ECL emission is synthesized. Lanthanide ions provide the cathode ECL emission, enhanced by the antenna effect and Tb→Eu energy transfer; the anode emits light from luminol using dissolved oxygen in water, requiring only the addition of K2S2O8 as a single co-reactant, resulting in fewer side reactions and a stable signal. The dual-potential ratiometric sensing utilizes the dual ECL signal for self-calibration, improving anti-interference and reliability; the single-luminescent dual-potential system achieves synchronous anode / cathode luminescence, simplifying the system and improving detection accuracy. By detecting changes in the ECL signal of different concentrations of analytes, the detection of microRNA-126 is achieved.

Provided herein is a methods for monitoring inflammatory disease activity in a subject comprising: (i) obtaining a sample from the subject; and (ii) measuring an autoantibody concentration in the sample using an immunoassay. Optionally, the inflammatory disease is rheumatoid arthritis (RA). Optionally, the autoantibody is an anti-citrullinated vimentin autoantibody (anti-Sa) or an anti-carbamylated protein autoantibody (anti-CarP). Optionally, the autoantibody concentration is correlated to a multi-disease biomarker activity (MBDA) score. Also provided herein are systems and kits for performing the method described herein.

The invention relates to a hybridoma cell strain secreting a rofecoxib monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. According to the invention, a hybridoma cell strain secreting the rofecoxib monoclonal antibody is obtained through screening by preparing a rofecoxib complete antigen and carrying out animal immune reaction and cell fusion steps, the hybridoma cell strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.46734. The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity, the IC50 value to rofecoxib is 20.0 ng / mL, the cross reaction rate to various rofecoxib functional analogues is smaller than 1%, the monoclonal antibody can be applied to preparation of rofecoxib detection products, and an efficient detection method and means are provided for detection of rofecoxib residues in food.

The invention discloses construction and application of a biosensor for triggering controlled release based on adjacent induced DNA strand displacement, and belongs to the technical fields of electrochemical luminescence, biological analysis and nano science. According to the invention, by integrating adjacent induced DNA strand displacement and controlled release strategies, the ultra-sensitive self-closing dual-mode biosensor taking the Au-Ag nanocluster as a luminous body is developed, and the ultra-sensitive self-closing dual-mode biosensor is used for trace detection of neuron-specific enolase. Due to the synergistic characteristic between the double metals, the Au-Ag nanocluster has high electrochemical luminescence (ECL) efficiency. Meanwhile, the reduction of S2O8 < 2-> is catalyzed by cyclic conversion between Fe < 2 + > / Mn < 3 + > and Fe < 3 + > / Mn < 4 + >, and a large amount of SO4 <-> is generated for ECL emission. The specific binding between the target antigen and the antibody can drive DNA strand displacement to open a blocked hole, so that the electrochemical probe falls off and the probe is quenched to release, the self-closing of an electrochemical signal and an ECL signal is realized, the detection sensitivity and accuracy of the sensor are effectively improved, and the sensor is suitable for immunoassay of various biomarkers.

The invention belongs to the technical field of preparation and rapid detection of composite materials, and particularly relates to a construction method and application of a high-sensitivity fluorescent probe for rapidly analyzing acrylamide in high-temperature processed food. According to the method, acrylic acid-N-succinimide ester (NAS) is grafted on carbon dots (CDs) and grows into a europium-based fluorescent metal organic framework (Eu-MOF) in situ, a ratio type fluorescent probe capable of being used for high-sensitivity detection is constructed, and compared with traditional technologies for detecting acrylamide in high-temperature processed food through liquid chromatography, enzyme-linked immunosorbent assay and the like, the ratio type fluorescent probe has the advantages that the ratio type fluorescent probe can be used for high-sensitivity detection; the method has the advantages of high sensitivity and high detection speed. The constructed ratio-type fluorescent probe can weaken the interference of external factors, has the advantage of strong anti-interference capability, and can improve the reliability of an analysis result; meanwhile, a visualization method is adopted, colorimetry is combined with a smart phone, and the method is easier to obtain by the public, so that the practical applicability is improved, and the application prospect is wide.

The present application relates to a kind of constant temperature shaking incubator for immunodetection kit and its shaking frequency adjusting method, belong to immunological analysis equipment technical field, including box, constant temperature module, shaking platform, drive mechanism and control system, still include: online temperature detection unit, set in the inside of box, for real-time acquisition kit current temperature signal;And optical detection unit, set in the above of shaking platform, for real-time monitoring the turbidity or light transmittance change of reaction liquid in kit, target temperature-time curve and target turbidity-time curve are built in control system, for receiving the feedback signal of online temperature detection unit and optical detection unit.In the present application, it solves the problem that traditional incubation equipment cannot dynamically adjust the shaking frequency according to the reaction process, leading to low reaction efficiency and large batch difference, significantly improves the sensitivity, specificity and result consistency of immunodetection.

The application relates to an immune analysis micro-fluidic chip, which comprises a chip body, the chip body defining an antibody reconstitution flow channel, a fixing area and a waste pool in sequence, the antibody reconstitution flow channel comprising first and second annular flow channels arranged alternately, the first and second annular flow channels being opposite in opening direction, the first annular flow channel having a circumferential angle of alpha between the two ends along the extension direction, the second annular flow channel having a circumferential angle of beta between the two ends along the extension direction, and alpha and beta satisfying 180 DEG < alpha <= 270 DEG and 180 DEG < beta <= 270 DEG. According to the immune analysis micro-fluidic chip, the reconstitution rate of fluorescent antibodies can be improved, and the combination rate of the fluorescent antibodies and antigens in samples can be improved.

The invention relates to a theophylline immunodetection reagent as well as a preparation method and a detection method thereof, in particular to a theophylline chemiluminescence immunodetection reagent as well as a preparation method and a detection method thereof, and the theophylline chemiluminescence immunodetection reagent comprises an anti-theophylline specific antibody and an indicating reagent for detecting an anti-theophylline specific antibody-theophylline conjugate, the anti-theophylline specific antibody is obtained by immunizing animals with theophylline immunogen. The theophylline immunogen has the beneficial effects that the theophylline immunogen has strong specificity and high immunogenicity, and the prepared anti-theophylline specific antibody has strong specificity and high titer; the chemiluminescence immunoassay reagent containing the anti-theophylline specific antibody can conveniently, rapidly and accurately determine the theophylline content in a sample, can simultaneously determine a plurality of samples on a full-automatic chemiluminescence immunoassay instrument, realizes high-flux rapid determination of theophylline, and has the advantages of high accuracy, strong specificity, high sensitivity and high sensitivity. And the accuracy and the detection efficiency are greatly improved.

The invention relates to an anti-ovarian antibody chemiluminiscence kit and a preparation method thereof. The kit comprises amino magnetic beads coated with anti-ovarian antibody recombinant protein, anti-human immune globulin labeled by alkaline phosphatase, an anti-ovarian antibody calibrator and a chemiluminiscence substrate. The method has the beneficial effects that the 7-day acceleration stability of the AOAb coated amino magnetic bead is improved to 95% or above, the signal-to-noise ratio is high, the correlation coefficient is greater than 0.999, the test repeatability CV is less than 3.5%, the reagent correlation and the magnetic bead stability can be better improved, and the test result is ensured to be accurate. By optimizing the amino magnetic bead cross-linking agent, 60 clinical samples are tested and compared with the result of a comparison manufacturer, the total coincidence rate is as high as 98%, and false positive can be effectively reduced. Meanwhile, a detection system is formed by matching with a Guilin superior chemiluminescence immunoassay analyzer IA-260, the testing amount per hour reaches 130 tests, and compared with a traditional colloidal gold immunochromatography method and a traditional enzyme-linked immunosorbent assay method, the detection speed is greatly increased, and the result optimization accuracy is greatly improved.

The utility model provides an immunodetection kit for drug-derived liver injury markers in blood, which relates to the technical field of medical detection and comprises a kit body, a drug mixing cavity is arranged on the left side of the upper portion of the kit body, a drug mixing box is fixedly connected in the drug mixing cavity, a detection display cavity is arranged on the right side of the upper portion of the kit body, and the drug mixing box is fixedly connected in the drug mixing cavity. The liver injury marker immunodetection kit comprises a kit body, a reagent mixing box is arranged in the kit body, a detection display cavity is arranged in the reagent mixing cavity, a rotary stirring pipe is rotationally connected in the reagent mixing box, a sealing plug is fixedly connected to the right end of the rotary stirring pipe, and an inter-cavity connecting hole is formed in a partition plate between the reagent mixing cavity and the detection display cavity in a penetrating mode. The buffer agent is contained in the medicament mixing box, the blood sampling cotton swab is hidden in the kit body, and a plurality of detection tools are combined into a whole, so that the use convenience of the kit can be improved, the tools can be prevented from being dispersed and lost, and the sanitary safety is guaranteed.

This invention provides, and in certain specific but non-limiting aspects relates to: assays that can be used to predict whether a given ISV will be subject to protein interference as described herein and / or give rise to an (aspecific) signal in such an assay (such as for example in an ADA immunoassay). Such predictive assays could for example be used to test whether a given ISV could have a tendency to give rise to such protein interference and / or such a signal; to select ISV's that are not or less prone to such protein interference or to giving such a signal; as an assay or test that can be used to test whether certain modification(s) to an ISV will (fully or partially) reduce its tendency to give rise to such interference or such a signal; and / or as an assay or test that can be used to guide modification or improvement of an ISV so as to reduce its tendency to give rise to such protein interference or signal; —methods for modifying and / or improving ISV's to as to remove or reduce their tendency to give rise to such protein interference or such a signal; —modifications that can be introduced into an ISV that remove or reduce its tendency to give rise to such protein interference or such a signal; ISV's that have been specifically selected (for example, using the assay(s) described herein) to have no or low(er) / reduced tendency to give rise to such protein interference or such a signal; modified and / or improved ISV's that have no or a low(er) / reduced tendency to give rise to such protein interference or such a signal.

1. Name of the product in this design: Interactive graphical user interface for displaying detection operations of a visual immunoassay system for electronic devices. 2. Purpose of this design: To demonstrate the data interaction of a visualized immune analysis system. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photo that best illustrates the design points: Interface change state diagram 1. 5. Purpose of the graphical user interface: This graphical interface is used to display interactive correlation data of the visualized immune analysis system. 6. Description of the changing states of the graphical user interface: The main view is the homepage, displaying the operation interface of the visual immunoassay system, including six function selection boxes: detection operation, detection selection, detection results, image window, device debugging, and parameter settings; Clicking the "detection operation" function selection box in the main view leads to the interface changing state diagram 1: This interface displays the relevant data for starting detection, single-step operation, reset OK, and single-step jogging; Clicking the "single-step operation" and "reset OK" function selection boxes in interface changing state diagram 1 leads to the interface changing state diagram 2; In interface changing state diagram 2, when both the "continuous operation" and "reset OK" function selection boxes are green, clicking "start detection" leads to the interface changing state 3: continuous detection state.