40results about How to "Reduce incubation time" patented technology

The invention relates to nutrient-rich red yeast rice bran and a preparation method thereof.The preparation method comprises the following steps that after rice bran is subjected to enzyme deactivation and sterilization treatment, the moisture is controlled to be 20%-30%; adding a composite enzymatic hydrolysate into the pretreated rice bran, uniformly stirring, and performing enzymolysis to obtain a semi-solid base material; the temperature of the semi-solid base material is controlled to be 36-40 DEG C, and the pH value is adjusted to be 5.0-5.5; inoculating monascus for culturing, turning over the yeast once every 10-12 hours, and culturing for 5-6 days to obtain a yeast material; spreading and culturing the koji material, and controlling the temperature of the koji material to be 30-35 DEG C; after the culture is completed, drying the yeast material until the moisture is below 10% to obtain the nutrient-rich red yeast rice bran; the monascus rice bran with high content of monacolin K can be obtained through the method, the technological operation is simple, the labor intensity of workers is reduced, the production period is short, and the method is suitable for large-scale application and has remarkable economic benefits and social benefits.

The invention relates to an easily-activated Ti-Fe-Y-Mn-Zr-Co-Zn-based hydrogen storage alloy and a preparation method thereof, belongs to the technical field of hydrogen storage alloy materials, and solves at least one of the problems of high activation difficulty, long activation period, poor hydrogen absorption and desorption dynamic performance and the like of a Ti-Fe-based hydrogen storage alloy in the prior art. The invention provides a Ti-Fe-Y-Mn-Zr-Co-Zn based hydrogen storage alloy easy to activate, the chemical formula of the hydrogen storage alloy is Ti 1.15, Fe 0.8, Y 0.03, Mn 0.35-x-y-z ZrxCoyZnz, x, y and z are atomic ratios, x is larger than or equal to 0.05 and smaller than or equal to 0.15, y is larger than or equal to 0.02 and smaller than or equal to 0.1, and z is larger than or equal to 0.02 and smaller than or equal to 0.1. The Ti-Fe-Y-Mn-Zr-Co-Zn based hydrogen storage alloy provided by the invention obtains good activation performance and hydrogen absorption and desorption performance by means of component design and preparation process improvement, can be activated at one time under the conditions of 30 DEG C and 3 MPa, and has the hydrogen storage capacity of more than or equal to 1.69 wt.%, the hydrogen absorption platform pressure of more than or equal to 0.39 MPa and the hydrogen desorption platform pressure of more than or equal to 0.3 MPa.

The invention discloses a near-infrared fluorescence sensor, a near-infrared fluorescence sensor array and a preparation method and application of the near-infrared fluorescence sensor array, and belongs to the technical field of biological medicine. The composite material comprises a single-walled carbon nanotube, a single-stranded DNA (deoxyribonucleic acid) and hydrogel, the single-stranded DNA is connected and modified on the surface of the single-walled carbon nanotube, and the single-walled carbon nanotube modified with the single-stranded DNA is fixed in the hydrogel. Bacterial metabolites enter the hydrogel and influence the structure of the single-stranded DNA, so that the fluorescence intensity of the single-walled carbon nanotube is influenced. A plurality of near-infrared fluorescence sensors form an array to quickly capture component change caused by growth of bacteria in an object to be detected, and respond to fluorescence signal change, so as to visualize whether the components change or not. Whether bacteria grow or not is judged through response information of the multiple sensors, so that the bacteria are rapidly recognized, and the method has the advantages of being high in sensitivity, short in response time and small in needed sample size.

The invention discloses an optimization method of a pea tissue culture regeneration system. The optimization method comprises the following steps: step 1, preparing pea aseptic seedlings; step 2, preparing an explant; step 3, inducing cluster buds; 4, carrying out bud elongation culture; step 5, rooting culture; and 6, domesticating and transplanting regenerated seedlings. By systematically optimizing the tissue culture conditions of the peas, an efficient regeneration system is established and is suitable for multiple pea varieties, and the problem of low regeneration efficiency in the prior art is solved. By means of the optimization method, researchers can obtain high-efficiency regenerated pea plants in a short time, the success rate and rooting efficiency of tissue culture regeneration are improved, meanwhile, the survival rate of regenerated seedlings is increased, and an important technical guarantee is provided for molecular breeding and gene modification of peas and other leguminous crops.

The invention belongs to the field of cell culture, and discloses an osteosarcoma organ culture medium and a gelatin planting method. The following components are added into the osteosarcoma organ-like culture medium: a B27 additive, an N-2 additive, a GlutaMAX additive, casein amino acid, N-acetylcysteine, nicotinamide, vitamin C, sodium pyruvate, human gastrin, Wnt3A, R-spondin 1 protein, Noggin protein, activin A protein, Forskolin, Y-27632, SB202190, CHIR99021, A83-01, h-EGF, FGF-10, HGF, VEGF A, IGF 1, IL-6, cyan-streptomycin and fetal calf serum. The osteosarcoma organoid culture medium provided by the invention can promote organoid cells to maintain relatively high multiplication capacity and maintain self-renewal, so that the amplification efficiency and the modeling success rate of the organoid are greatly improved.

The invention provides a rapid drug sensitivity detection kit for a breeding site, and belongs to the technical field of drug sensitivity detection. By preparing the penaeus vannamei polypeptide and adding the penaeus vannamei polypeptide into the casein tryptone liquid culture medium, the penaeus vannamei polypeptide has a remarkable promotion effect on the growth of common vibrio parahaemolyticus, vibrio harveyi and edwardsiella tarda in aquaculture, and the bacterial culture time can be shortened. The penaeus vannamei polypeptide is added into a drug sensitivity detection solution to be matched with a triphenyltetrazole chloride color developing agent and a drug sensitivity piece detection system, the prepared rapid drug sensitivity detection kit is used for drug sensitivity detection of clinical diseased prawn samples, and the drug sensitivity detection result can be judged only within 5 hours. The result judgment time is 4 hours earlier than that of a common drug sensitivity detection kit without the prawn polypeptide, and the problem of on-site rapid drug sensitivity detection of an aquaculture farm can be solved.

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

The tuberculosis infection T cell immune evaluation system comprises a microfluidic immune reactor, a pipettor, a detection antibody, a detection tracer SA-PolyHRP, a luminol chemiluminescence substrate, a chemiluminescence detection unit and a computer, the microfluidic immune reactor is coated with an interferon capture antibody, and the microfluidic immune reactor is coated with a chemiluminescence detection unit. The pipettor is used for sucking a blood sample, a detection antibody, a detection tracer SA-PolyHRP and a luminol chemiluminescent substrate into the microfluidic immune reactor; the chemiluminescence detection unit collects a chemiluminescence image signal and converts the chemiluminescence image signal into a quantitative value signal, and the quantitative value signal is converted into an equivalent standard interferon concentration through a standard curve so as to evaluate the T cell immune protection capability of the sample; according to the tuberculosis infection T cell immune evaluation system and evaluation method provided by the invention, through a high-sensitivity detection platform, the stimulation incubation in a trace blood sample can be realized, the incubation time can be shortened, and the clinical demand on the blood volume of a patient can be reduced, so that some special crowds can also perform IGRA detection, and the system and the method are rapid, flexible, stable and reliable.

The utility model discloses a device for preventing temperature and humidity leakage of an incubator, which relates to the technical field of incubator accessory equipment and comprises a case, a fixed plate is fixedly arranged on one side in the case, a heater is fixedly arranged on one side in the fixed plate, the other side of the fixed plate is symmetrically communicated with air inlet pipes, and fans are rotatably arranged in the air inlet pipes; one end of the air inlet pipe communicates with the fixing frame, and a drawer is slidably arranged in the fixing frame. A flitch is hinged to the upper end face of the fixing frame, locking pieces are symmetrically and rotationally connected to the front of the flitch, embedded blocks are fixedly connected to the tail ends of the locking pieces, and embedded holes are formed in the positions, corresponding to the embedded blocks, of the lower end face of the fixing frame. A gasket is mounted at the joint of the air inlet pipe and the fixed frame; the air inlet pipes symmetrically communicate with the sides, away from the heaters, of the fixing plates. The flitch is hinged to the upper end face of the fixing frame and close to the drawer. The problems that an existing partial device used for improving the air tightness of the incubator is complex in structure and inconvenient to operate are solved.

The present invention relates to the technical field of organoid culture, and discloses a method for culturing goose kidney organoids, wherein the method firstly takes goose embryos, separates primary kidney tissues, washes, crushes, digests, filters, and washes twice to obtain primary cells, then mixes the primary cells with matrix gel, coagulates, obtains a colloidal mixture, and then places the mixture in a culture medium containing goose serum and cultures it for at least 10 days to obtain goose kidney organoids; wherein, the method comprises: 4 The primary cells are mixed with 70 μL of matrix gel and solidified to obtain a colloidal mixture. Through the above design, the goose kidney organoid obtained in this application is a 3D cell culture system, which is highly similar to the tissue or organ derived from the body, can replicate the complex spatial morphology of differentiated tissues, and can show the interaction between cells and cells, and cells and matrix. In addition, the application also discloses a goose kidney organoid and its application.

The invention provides a preparation method of a bionic senescence brain-like organ for simulating the brain of the elderly, and the preparation method comprises the following steps: inducing human induced pluripotent stem cells into embryoid bodies, and inducing and differentiating the embryoid bodies EB into brain-like organs; the brain-like organ is placed in a culture medium containing bleomycin, the brain-like organ is induced to differentiate the senescence phenotype, and the bionic senescence brain-like organ with senescence characterization is prepared, so that the physiological condition of senescence brains of people is simulated. According to the preparation method of the senescence brain-like organ, the time required by the senescence of the brain-like organ in a natural state is greatly shortened, the preparation method is simple and easy to implement, the physiological condition of the brain of the elderly people can be simulated more truly, related basic research, disease modeling and drug screening are carried out, and the preparation method is suitable for popularization and application. The method has the advantages which cannot be achieved by traditional in-vitro culture and animal experiments, and is wide in application prospect.

The invention relates to the technical field of plant polyploidy breeding, and provides a method for inducing dandelion chromosome doubling by colchicine, which comprises the following steps: sowing and germinating to obtain seedlings, and coating plant growing points of the seedlings with a colchicine solution for induction treatment to obtain dandelion with doubled chromosomes. According to the technical scheme, the problems that traditional breeding of the alkaline land dandelions is difficult, novel breeding technologies are deficient, good varieties are few, biomass of some high-quality varieties is small, and industrial development of the alkaline land dandelions is affected in the prior art are solved.

The invention relates to a method for avoiding generation of silica gel in red mud leachate, which comprises the following steps: drying red mud, crushing, grinding and sieving, dissolving powdery red mud in water to prepare slurry, and standing and layering to obtain red mud concentrated slurry with a dealkalized lower layer; the method comprises the following steps: reselecting red mud concentrated slurry to obtain red mud concentrate and red mud tailings, further treating the red mud concentrate by adopting biological flocculation precipitation to obtain desiliconized red mud concentrate, carrying out chemical leaching or biological leaching on the desiliconized red mud concentrate, adding an H2O2 solution into a leaching system, standing or filtering a leaching solution after leaching is completed, and recycling the red mud concentrate and the red mud tailings. Valuable metal elements in the filtrate are recovered; and dissolving trace silicon remained in the desiliconized red mud concentrate, and performing secondary mineralization to form crystalline quartz precipitate. According to the method, silicon-containing minerals in the red mud are effectively removed before leaching of the red mud, silicon is removed from the source, silicon elements remaining in the red mud in the leaching process are dissolved and then secondarily mineralized into crystalline quartz to synchronously precipitate, and the negative influence of silica gel on leaching of the red mud can be inhibited or eliminated to the maximum extent.

The invention discloses a CHO (Chinese hamster ovary) cell and a suspension process method, which are characterized in that a stable high-expression cell strain is obtained by improving a target expression vector, and the protein expression quantity of 1g / L is realized. The improved CHO cell can be suitable for a WAVE wave bioreactor and serum-free culture, contamination is effectively prevented, interference of exogenous factors is reduced, the level of related impurities such as endotoxin in a cell expression product is reduced, the operation steps are simplified, the occupied culture space is reduced, meanwhile, the culture time is shortened, and the culture efficiency is improved. Therefore, the culture efficiency is improved, bacterial contamination is effectively prevented, the endotoxin level in the product is reduced, and the production cycle is greatly shortened.

The invention discloses a rapid seedless grape breeding method in the technical field of plant cultivation, which comprises the following steps: step 1, selecting male and female parents and hybridizing: selecting seedless excellent varieties as female parents and male parents respectively, performing artificial inflorescence emasculation on the female parents by using tweezers, bagging and marking, and performing artificial pollination; step 2, embryo rescue: shearing and taking back the hybridized clusters, washing the fruits with running water for 2-4 hours, transferring the fruits into a bottle for disinfection, stripping ovules, inoculating the taken ovules into an embryo development culture medium for dark culture, and after 8-10 weeks, cutting off the embryos and inoculating the embryos into an embryo germination culture medium for light culture; and step 3, transplanting and seedling hardening. The method is simple and easy to operate, through the design of the functional scissors, a sample is disinfected and coated with a culture solution while shearing is achieved, then the sample is disinfected, the activity of the sample is kept, and a good foundation is laid for subsequent seedless grape cultivation.

The invention discloses a method for cultivating a good camellia chrysantha strain through bare-root transplanting and grafting of a camellia adult tree. The method comprises the following operation steps: (1) stock preparation and bagging; (2) grafting; (3) management after grafting; (4) field planting and management. According to the method, the stock stump is excavated in a completely bare-root mode, the treatment method of stump root systems, branches and the like is easy to operate, transportation is convenient, labor is little, cost is low, and centralized grafting management is facilitated; the adopted pulp root treatment can provide nutrition for the rootstock, can also play a role in sterilization and insect killing under specific humidity, and can inhibit the possibility that the excavated rootstock suffers from diseases and pests as much as possible; according to the method, stock transplanting and scion grafting are conducted at the same time, the cultivation time of large-specification camellia nitidissima plants can be effectively shortened, the cultivation cost is reduced, and early yield and high yield of grafted trees are achieved.

The invention discloses a method for breeding large-size high-quality young crayfish in early spring. The method comprises the following steps: (1) selecting and cleaning a pond; (2) nutrient enrichment culture of adult shrimps; (3) environment regulation and control; (4) parent screening; and (5) breeding young prawns. According to the method, independently researched and developed pellet feed and hermetia illucens protein source fermented feed are utilized to strengthen parents, gonad development of the crayfish is forcibly promoted by utilizing the environment, and the crayfish parents are promoted to mate and lay eggs in advance; an independently researched and developed hermetia illucens protein source pellet feed is used for cultivating young crayfish and cultivating early spring large-size crayfish fry, and a foundation is laid for staggered breeding of crayfish; according to the method, large-scale supply of large-size high-quality seedlings in spring is achieved, the technical bottlenecks that the seedlings of the premna davidiana are difficult to obtain and adult shrimps are excessively concentrated to appear on the market in the current industry are solved, and healthy and sustainable development of the industry is promoted.

The invention discloses a cultivation device capable of shortening mushroom cultivation time, and relates to the technical field of mushroom planting.The cultivation device comprises a mushroom fruiting room and two placing frames and further comprises humidity adjusting mechanisms arranged on the two sides of the inner wall of the mushroom fruiting room; according to the cultivation device capable of shortening the mushroom cultivation time, the temperature and humidity of all areas in the mushroom fruiting room are detected in real time through a plurality of temperature and humidity sensors, the environment in the mushroom fruiting room is intelligently regulated and controlled according to the detection result of the sensors and the cultivation stage, and the atomization spray head is driven by the adjusting assembly to spray the mushrooms into the mushroom fruiting room. Meanwhile, an air outlet head is driven by a lead screw assembly and an electric push rod to uniformly heat and raise the temperature in the fruiting room, so that the difference of the environment where the mushroom sticks on the placement frame are located is small, the mushroom sticks are not easily influenced by external unstable weather, and the mushroom sticks are neat in fruiting and good in consistency; lentinus edodes cultivation time is shortened, and productivity is improved.

The invention provides a culture medium for rapidly culturing psychrophilic bacteria in cow's milk, the culture medium is prepared from deeply hydrolyzed lactoprotein, glucose, agar and ultra-high temperature sterilized milk, and the material ratio of the deeply hydrolyzed lactoprotein to the glucose to the agar to the ultra-high temperature sterilized milk to deionized water is (1.0-10.0 g): (0.2-3 g): 15.0 g: (50-500 ml): (950-500 ml). The invention also provides a preparation method of the culture medium for rapidly culturing psychrophilic bacteria in cow milk. The culture medium can effectively shorten the culture time and improve the detection and research efficiency in the aspect of rapidly culturing the psychrophilic bacteria in cow milk, the cultured microorganisms are basically the same as MPC and PCA common psychrophilic bacteria culture media in variety, and the culture medium has wide application prospects in the fields of food microorganism detection, dairy product quality control and the like.

This invention provides an expression vector pHH40 and its application in the construction of engineered *C. truncatula* strains, belonging to the field of microbial engineering technology. pHH40, as an expression vector, can significantly improve the success rate of exogenous gene expression in *C. truncatula*. Furthermore, we used pHH40 to introduce the heat shock protein encoding gene lphsp into *C. truncatula*, and the resulting engineered *C. truncatula* strains can tolerate ethanol concentrations up to 14%, and can more quickly initiate and complete malolactic (MLF) fermentation in wine production, shortening the wine production cycle.

The invention discloses a management system and management method compatible with a warehouse area and a material cabinet, and the system comprises a warehouse setting module, a purchase warehousing module, a warehousing inspection module, an in-process inventory module, a warehousing inventory module, a material delivery module, and a material return module. The purchasing and warehousing module is used for storing information of all purchased materials, the warehousing inspection module is used for storing inspection information of warehousing and feeding materials, the in-inspection inventory module is used for storing information of materials being detected, and the warehousing inventory module is used for storing information of warehousing materials. The material warehouse-out module is used for storing warehouse-out material information, and the material warehouse-out module is used for storing returned material information. The system has the advantages that compatible management of the storage locations or the material cabinets in the warehouse can be realized, the management efficiency is improved, and the management cost is reduced.

This invention discloses an integrated device and a method for rapid cultivation of aerobic granular sludge suitable for low-concentration domestic sewage, along with its application. The device comprises a batch reactor body integrating the following functional units: a full-area water distribution unit, including a main inlet pipe and distributed water distribution components; one end of the main inlet pipe is connected to the sewage source, and the other end extends to the bottom of the reactor cavity; the distributed water distribution components are connected to the main inlet pipe and equipped with multiple water distribution holes; a dual-zone aeration unit, including an air pump, a bottom aeration unit with a wide range of adjustable air volume, and a middle aeration unit with precise air volume control; a flow field optimization unit, located at the upper part of the reactor cavity and connected to the main inlet pipe; and a timing control unit, electrically connected to the full-area water distribution unit and the dual-zone aeration unit. The advantages of this invention are: by creating sedimentation selective pressure through dual-zone aeration to achieve sludge stratification, enhanced denitrification of heavy granular sludge, and removal of excess phosphorus from light flocculent sludge, it also has the advantages of shortening the cultivation cycle of aerobic granular sludge and highly efficient denitrification and phosphorus removal.

The application provides a chrysosporium inosporum breeding method and relates to the technical field of edible mushroom cultivation. The breeding method is as follows: the chrysosporium fruiting body is hung and cultured, and after the spores in the fruiting body are separated out, the spores are moved to a flat plate culture medium to culture the chrysosporium single colony, two chrysosporium single colonies are randomly picked and cross lines are drawn on the same clean flat plate culture medium to carry out hybridization culture, and the liquid strain of phanerochaete chrysosporium is simultaneously introduced as a mycelium growth auxiliary, is evenly smeared on the culture medium, and the ear is induced. The breeding method solves the technical problems of the existing cultivation method, such as complex operation, tedious steps, high requirement for equipment and long cultivation time, and realizes the technical effects of fast spore culture speed, simple operation, low equipment requirement and meeting the needs of factory production.

A method for rapidly enriching sulfur autotrophic denitrifying bacteria without inoculating activated sludge belongs to the field of deep denitrification of tail water of landfill leachate MBR. The present invention uses a sulfur autotrophic denitrification filter as a reactor, and a sulfur elemental granular filler as a place for microbial enrichment; the sulfur source required for the growth of sulfur autotrophic denitrifying bacteria is provided by the sulfur elemental granular filler; the water produced by the landfill leachate MBR is used as the influent, and sodium bicarbonate is used as the exogenous alkalinity to adjust the pH of the influent to 8-9; the water inlet mode adopts continuous flow, and the hydraulic retention time is set to 20h. After one week of continuous cultivation, the sulfur autotrophic microorganisms have been basically enriched, and the total nitrogen removal rate reaches 40%; the total nitrogen removal rate of the sulfur autotrophic denitrification filter in the third week can basically reach more than 92%. The present invention does not need to inoculate exogenous microorganisms, reduces the generation of miscellaneous bacteria during the cultivation process, and greatly shortens the acclimation time of sulfur autotrophic denitrifying bacteria, and the total nitrogen removal rate can reach more than 92%.

This invention belongs to the field of organic wastewater treatment technology. To address current issues such as carbon emission reduction and energy shortages, it provides a method for promoting lipid production and wastewater purification using nitrogen and phosphorus in wastewater from *Scenedesmus obliquus*. *Scenedesmus obliquus* is inoculated into artificial wastewater and cultured. While adding salicylic acid at a concentration of 20-40 mg / L, low-intensity ultrasound (200 W, 40 kHz frequency) is applied for 5-10 minutes. Cultivation continues until nitrogen and phosphorus in the wastewater are completely consumed. The nitrogen and phosphorus removal rate is measured, and the algae are collected to produce algal powder for oil extraction. Coupled with artificial wastewater, this method achieves wastewater resource recovery while saving costs. The nitrogen and phosphorus removal rate in the wastewater is as high as approximately 90%, the oil content of *Scenedesmus obliquus* is higher than 60%, and the oil yield is as high as 40 mg / L·d. ‑1 .

The invention discloses an application of a single base mutation of a bovine DDX58 gene in anti-viral infection, and belongs to the technical field of anti-viral infection prevention and treatment. The single base mutation of the bovine DDX58 gene provided by the invention can significantly affect the replication of RNA viruses and the expression of IFNBI. When the 858th amino acid of the DDX58 protein is asparagine, the expression of IFNBI is significantly increased, thereby inhibiting the replication of RNA viruses and improving the anti-viral ability; the site is highly linked to the anti-RNA virus infection ability; the single / base / amino acid mutation of the bovine DDX58 gene provided can be used as a linkage site for bovine resistance to RNA virus infection, and is used for the genotype breeding of anti-viral dairy cow varieties, thereby simplifying the breeding method and shortening the breeding time.

The invention discloses a cultivation method of a nitrogen-efficient rice variety, which comprises the following steps: selecting a breeding material 898 containing an OsTCP19DY gene and a breeding material Ganxiangxi rice No.1 containing an OsCERK1DY gene as donor parents, and respectively hybridizing the donor parents with coma parents as receptor parents; kASP molecular markers of the nitrogen efficient genes OsTCP19DY and OsCERK1DY are developed, designed and identified for molecular marker-assisted selection, and the two nitrogen efficient genes OsTCP19DY and OsCERK1DY are polymerized in a breeding material through continuous backcross, selfing and convergent hybridization; according to the breeding material HZ-OsTCP19DY / OsCERK1DY cultivated by using the cultivation method disclosed by the invention, the utilization efficiency and the yield of nitrogen elements under a low-nitrogen condition are remarkably improved.

The invention relates to the technical field of microorganisms and agriculture, in particular to a functional bacterium resource screening and raw material treatment method, which comprises the following steps: step 1, sample treatment: collecting a sample from a sampling environment, and pretreating to obtain a microbial suspension; step 2, separation and purification: carrying out five times of streak purification on the microbial suspension by adopting a streak separation method to obtain a purified strain; 3, primary screening of functional bacteria: further culturing the purified strains, and screening out bacterial colonies with large transparent circle diameters to obtain primary screening functional strains; step 4, re-screening the functional bacteria: re-screening the primarily screened functional bacteria to obtain re-screened functional bacteria; and step five, detecting functional bacteria: adding the functional bacteria subjected to secondary screening into a specific fermentation product to detect the activity of enzyme production of the functional bacteria. The functional bacterium resource screening method disclosed by the invention can be used for efficiently screening microbial strains with specific functions, and is beneficial to quickly positioning and obtaining valuable microbial resources.

The invention relates to a fairy shrimp shelling method which comprises the following steps: (1) pretreating fairy shrimps with water to obtain pretreated fairy shrimps; and (2) mixing the pretreated fairy shrimps with a shelling agent to obtain shelled fairy shrimps. According to the shelling method, egg shells and egg membranes of fairy shrimps can be fully removed, the hatching time of fairy shrimp eggs can be shortened, the fairy shrimp eggs cannot be damaged in the re-shelling process of the shelling agent, and the situation that the hatching rate and the survival rate of the fairy shrimp eggs are reduced is avoided.

The present invention provides a method for increasing cell growth and protein expression in in vitro cell culture using recombinant growth factors produced by genetically engineered yeast strains. A recombinant growth factor is introduced into a culture medium of a cell to be cultured, thereby increasing the growth rate or protein expression rate of the cultured cell. In one embodiment, recombinant growth factors are used that (i) genetically the same or similar species as the cultured cell and / or (ii) genetically the same genus as the cultured cell. In another embodiment, recombinant growth factors having engineered amino acid sequences are used to improve stability, biological activity, or other characteristics that promote cell growth and / or protein expression of the cultured cells.