1147results about How to "Reduce incubation time" patented technology

A film-forming method includes forming a tungsten film or a tungsten oxide film on an object to be processed, forming a seed layer on the tungsten film or the tungsten oxide film, and forming a silicon oxide film on the seed layer, wherein the seed layer formed on the tungsten film or the tungsten oxide film is formed by heating the object to be processed and supplying an aminosilane-based gas to a surface of the tungsten film or the tungsten oxide film.

The disclosed electrochemical screening and early diagnostic device for malignant tumor comprises: an immune detection chip (1) connected to a time-resolution multichannel potentiostat (2) that connects to a data process and display system (3) by an interface. Wherein, the chip (1) comprises eight working electrodes fixed different antigen films and Ag wire (a), Ag/AgCl reference electrode (b), a carbon-pair electrode (c), and an insulation film (d). This invention needs low cost, has intelligence and fit to fast detection.

The invention relates to a technology for high-density fermentation of Lactobacillus casei (Lactobacillus casei Zhang) with prebiotic efficacy, which comprises optimal controls on a proliferation culture medium and the condition of a fermentation process, and a technology for preparing high-vitality freeze-dried powder of the strain. With the technology, the freeze-dried powder of the Lactobacillus casei with more than 10<11> cfu per milliliter of viable bacteria can be obtained, and the freeze-dried powder can be directly applied to the preparation and the application of a probiotic hametsz and a probiotic preparation of the strain.

The invention discloses a biochip incubation reactor, comprising a reaction chamber, an upper cover plate and a lower cover plate that are symmetrically arranged in the reaction chamber. An inner wall of the reaction chamber is equipped with a boss which is molded in one body with the reaction chamber and has a hollow structure to be used as an incubation reaction chamber. A gap used for installing a biochip is arranged between a lower surface of the upper cover plate and the boss; and a gap used for installing a biochip is also arranged between an upper surface of the lower cover plate and the boss. The biochip incubation reactor of the present invention has a simple and novel structure, and is convenient for operation. During the reaction, solution volatilization can be avoided, and high strength oscillation can be realized, so as to increase reaction efficiency, reduce incubation time, and enhance experiment sensitivity, singularity and reappearance. Both the upper cover plate and the lower cover plate of the reactor can be pasted with biochips, so that incubation of double biochips can be completed simultaneously to save samples at least by half compared with a traditional method.

A method for producing superior edible fungi culture medium by using manioc waste and acid-sludge as raw materials, belonging to the technical field of comprehensive utilization of the manioc waste and the acid-sludge, and edible fungi cultivation. In the invention, the manioc waste and the acid-sludge, two common leftover bits and pieces in fermentation and food industries, are scientifically compounded to serve as edible fungi culture medium, and the invention can be used for the production of all edible fungi culture media. The invention, on the basis of reducing culture medium raw material cost by over 50%, can shorten the culture period by 10-20% and increase the yield by 20-30%. The method resolves the problems of current low utility ratio of the manioc waste and the acid-sludge, serious environment pollution and the like, converts waste into treasure, and has high social and economic benefits.

The invention discloses a culture medium for culturing fruiting bodies of Antrodia cinnamomea and a cultural method thereof. The culture medium comprises potato dextrose broth (PDB) with a concentration of 1-2%(w/v), glucose with a concentration of 2.8-3.6%(w/v), agar with a concentration of 2.2-4%(w/v) and a Chinese medicine composition with a concentration of 16.7-4.%(w/v). The cultural method comprises the following steps: preparing a solid culture medium according to the ratio of the ingredients, inoculating an Antrodia cinnamomea strain to the solid culture medium, putting the solid medium containing the Antrodia cinnamomea strain in a specific environment to culture for a certain period to generate the fruiting bodies of Antrodia cinnamomea, wherein the fruiting bodies of Antrodia cinnamomea obtained by the cultural method have same index components as wild Antrodia cinnamomea.

Photodynamic therapy to prevent secondary cataracts is effected using photosensitizers such as green porphyrins as photoactive agents to destroy remnant lens epithelial cells.

The invention discloses a method for fermentation-membrane separation combined production of long-chain dicarboxylic acid and especially relates to a method for high yield production of dodecanedioic acid (DC12). The method comprises introducing a Candida viswanathii culture seed liquid into a liquid fermentation medium mixed solution which contains 5-40% (v/v) of C10-C18 n-alkane and 95-60% (v/v) of saccharic multielement substrate as a growth carbon source and has pH of 5.0-8.5, carrying out culture on the mixed solution under conditions of a temperature of 24-40 DEG C and ventilatory capacity of 0.1-3.0vvm for 42-194h, starting a fermentation-membrane separation combined device, returning the cells separated by the combined device into a fermentation cylinder, feeding the permeate liquid separated by the combined device into an extraction unit, preparing long-chain dicarboxylic acid, simultaneously, supplying a medium into the fermentation cylinder and carrying out fermentation. In n-dodecane transformation production of DC12, the method has an acid yield of 250g/L and an acid production rate of more than 1.6g/h.L, improves microbial density in fermentation, shortens a fermentation period, improves dicarboxylic acid production intensity and returns the unused n-dodecane into the fermentation unit.

The invention discloses an overhead-layering rapid propagation method of camellia chrysantha seedlings and belongs to the technical field of camellia chrysantha propagation. The method is characterized by including the steps of (1) seed tree branch selection, (2) branch girdling, (3) medium treatment, (4) medium wrapping, (5) post-layering management and (6) plant shearing and planting. The overhead-layering rapid propagation method of the camellia chrysantha seedlings is easy and convenient to implement, short in seedling growing time, high in rate of survival and suitable for camellia chrysantha propagation.

The invention discloses a bio-enzyme and microorganism containing compound feed additive and a preparation process thereof, which solve the problems of high production cost and less viable count of beneficial microorganisms in the prior art. The bio-enzyme and microorganism containing compound feed additive is characterized in that the additive is prepared by compounding a viable organism preparation (prepared by carrying out liquid fermentation and low-temperature drying on bacillus subtilis and brewer's yeast) with an enzyme preparation (prepared by carrying out solid fermentation and drying on trichoderma reesei and Aspergillus niger); and the viable count of beneficial microorganisms in each gram of the product is greater than 8*10<9> cfu/g, the cellulase activity (refer to the quantity of cellulose degraded by each gram of the product per minute) is greater than 500 mg, the alpha-amylase activity (refer to the quantity of starch hydrolyzed by each gram of the product per minute) is greater than 3000 mg, and the protease activity (refer to the mount of an amino acid generated by caseins hydrolyzed by each gram of the product per minute) is greater than 400 mg. The bio-enzyme and microorganism containing compound feed additive disclosed by the invention has the advantages that: the feed utilization rate of livestock can be improved in a short term, the intestinal digestive flora of livestock is improved and balanced, the breeding cost is reduced, and the feed conversion rate and the animal production performance are improved; and meanwhile, the additive is simple in preparation process, low in production cost, and suitable for industrialized production.

A thin film forming method which forms a seed film and an impurity-containing silicon film on a surface of an object to be processed in a processing container configured to be vacuum exhaustible includes: performing a first step which forms the seed film by supplying a seed film raw material gas including at least any one of an aminosilane-based gas and a higher silane into the processing container; and performing a second step which forms the impurity-containing silicon film in an amorphous state by supplying a silane-based gas and an impurity-containing gas into the processing container.

The invention provides a word art potted landscape cultivation technique which comprises comprehensive word art, including a foreign language word art, a Chinese character word art and an Arabic numeral word art; a word art potted landscape is easily available from trees, shrubs, vines and perennial trailing herbaceous plants, and also can be obtained by a plurality of seedling growing methods including seed propagation, cutting propagation, mound layering, ring-cutting reproduction, root stump propagation and the like; the technology combines a potted landscape cultivation technique and a typeface calligraphic art and utilizes nursery stock growth characteristics; by adopting a technical method that the growing nursery stock is reversely bound along a typeface character die rail and the turning position is subjected to twisting or stem-cutting and bud-retaining, a novel word art potted landscape can be obtained by methods including cutting off the stems, grafting, pruning and modeling, and artificially decorating and the like.

The present invention relates to a method for producing clinically effective quantities of human adipose tissue-derived stromal cells for treating fistulas and a composition made with the same. The method of the present invention can efficiently produce clinically effective number of adipose tissue-derived stromal cells within a short period by improving conventional standard culturing methods. The adipose stem cells composition containing the adipose tissue-derived stromal cells obtained by the method of the present invention exhibit superior multipotency and immunomodulatory activity over those of a cell composition produced by conventional methods, and thus is more suitable for treating fistulas. The cell composition of the present invention has excellent clinical usages especially since immune response is suppressed in allogenic adipose-derived stem cells.

The invention relates to a submerged fermentation culture method of straw mushroom liquid strains and culture mediums thereof. The invention solves the technical defects of the prior art, such as long mother species propagation time, poor hypha quality, long fungus ball production time, different fungus ball sizes, higher production cost and the like. The method is used for the submerged fermentation culture of the straw mushroom liquid strains. The method comprises the following steps: 1. carrying out mother species culture in a special culture medium of straw mushroom mother species; 2. carrying out transition culture in a special culture medium of straw mushroom stocks; 3. homogenizing the stocks to obtain homogeneous succulent strains; 4. carrying out primary shaking culture and amplification in a shaking liquid culture medium; 5. carrying out secondary shaking culture and amplification; and 6. carrying out fermentation treatment in a submerged fermentation liquid culture medium to obtain the finished products of the straw mushroom liquid strains. The invention relates to the special culture medium of straw mushroom mother species, the special culture medium of straw mushroom stocks, the shaking liquid culture medium and the submerged fermentation liquid culture medium.

The invention discloses an aerobic granular sludge culture method suitable to low-concentration domestic sewage, belonging to the field of environmental protection and resource comprehensive-water pollution prevention and control technology. The method comprises two stages of material supplement operation and low-load operation and particularly includes the following steps of: firstly, inoculating flocculent active sludge to an aerobic granular sludge culture reactor, and then supplementing and adding an easily degradable carbon source to low-concentration domestic sewage to adjust inflow COD(Chemical Oxygen Demand) to be 600-800mg/L; culturing active sludge in an inflowing-aerating-precipitating-water draining sequential operation mode into semi-cultured or completely-cultured aerobic granular sludge; and then keeping culturing with the low-concentration domestic sewage; and finally carrying out low-load acclimation and culture on aerobic granules in an inflowing-aerating-precipitating-standing-water draining sequential operation mode. The sludge granules have large size and high removal rate up to 90% to COD in the low-concentration domestic sewage, as well as high ammonia and nitrogen removal rate of more than 93%.

A method for fabricating a capacitor includes: forming a storage node contact plug over a substrate; forming an insulation layer having an opening exposing a surface of the storage node contact plug over the storage contact plug; forming a conductive layer for a storage node over the insulation layer and the exposed surface of the storage node contact plug through two steps performed at different temperatures; performing an isolation process to isolate parts of the conductive layer; and sequentially forming a dielectric layer and a plate electrode over the isolated conductive layer.

The invention discloses a bacillus amyloliquefaciens D2WM as well as a preparation method and application of bacillus amyloliquefaciens D2WM. The bacillus amyloliquefaciens D2WM comprises the steps of A, bacillus separation and purification, namely collecting after inoculating a slope; B, preliminary screening of bacillus, namely sequentially selecting strains having antagonistic effects on Erwinia and camellia oleifera diseases by using a point connection method and a plate confrontation method; and C, secondary screening of bacillus, namely culturing the preliminarily-screened strains with the antagonistic effects in an LB liquid culture medium by using a cup-plate method, and screening the strains simultaneously having the antagonistic effects on main diseases of Erwinia and camellia oleifera through an antagonistic bacterium fermentation liquid and sterile filtrate. When being applied to preparation of drugs for treating or preventing soft rot of vegetables and camellia oleifera diseases, the bacillus amyloliquefaciens has a broad-spectrum antagonistic effect, high in growth speed, capable of efficiently antagonizing anthracnose of camellia oleifera, leaf spot diseases of camellia oleifera, black spot of camellia oleifera, canker of camellia oleifera and various diseases and remarkable in antibacterial effect, the culture time for general bacteria and fungi is greatly shortened, and the production cost is reduced.

A method and system is provided which uses cleavable linkers to detect an analyte in an immunoassay. The use of linkers in ELISA and similar immunoassay protocols allows for a reduction in wash steps, incubation time, and potential for user error. The linkers create an environment that allows for intramolecular binding kinetics for quickly binding an analyte to two antibodies. The system works with ELISA and other similar protocols, and one embodiment of the invention does not require the binding of antibodies to a solid support. The disclosure also provides a method of making the system of cleavable linkers for use in a variety of immunoassays.

The invention discloses a method for producing long-chain binary acid through fermentation separation coupling, in particular to a method of high-yield dodecanedioic acid (DC12). According to the technical scheme, the method includes the steps that candida viswanathii is cultivated into a seed solution which is led into 5-40% (v/v) n-alkane and 60-95% (v/v) liquid fermentation medium mixture, wherein the pH of the n-alkane ranges from 5.0 to 8.5, and 10 to 18 carbon atoms are contained in the n-alkane; a saccharic multielement substrate serves as a growth carbon source of the liquid fermentation medium mixture; mixing liquid is cultivated for 42 h to 194 h under the conditions that the temperature ranges from 24 DEG C to 40 DEG C, and the air passing amount ranges from 0.1 vvm to 3.0 vvm, a centrifugal fermentation separation coupling device or a plate frame filtering fermentation separation coupling device is started, cells passing through the separation coupling device are circulated back to a fermentation cylinder, and clear liquid passing through the separation coupling device enters an extraction link to prepare long-chain binary acid; and meanwhile the fermentation process is continuously carried out on a cultivation medium in a fermentation tank. The method is used for converting n-dodecane so as to produce the DC12, the produced acid reaches 240 g/L, and the acid production rate is larger than 1.5 g/h.L.

The invention provides a process for producing the oriental lily sterilized seed globule in the bottle, comprising: choosing explant, sterilizing, inducing culture and getting the sterilized stem tip, then breeding culture, subculture, and producing the sterilized seed globule in the bottle directly. The invention produces the sterilized seed globule in the bottle directly to avoid the problem of long period of ball forming and the accumulation of virus existing in the oriental lily, the seed globule can be planted in the soil when out of the bottle, and the survival rate is high. The breeding procedure of the sterilized seed globule is simplified by modifying and optimizing the whole producing procedure for the lily seed globule, and the breeding time of sterilizing the oriental lily is shortened greatly, the quality of seed globule is improved when the producing cost is decreased, which reinforces market competitive power of the domestic self-breeding seed globule and is suitable for the mass production of the oriental lily sterilized seed globule.