5results about How to "Reduce incubation time" patented technology

The invention discloses a method for cultivating a heat-resistant broad-spectrum durable rice blast-resistant rice breeding material based on polygene polymerization. According to the method, a high-heat-resistance and high-dominance indica-japonica hybrid indica rice restorer line is selected as a recurrent parent, a breeding intermediate material for polymerizing complementary broad-spectrum rice blast resistance genes Pigm and Pi-jx is used as a donor parent, and polymerization of the heat-resistance and broad-spectrum lasting rice blast resistance genes Pigm and Pi-jx is realized through a method of combining molecular breeding with conventional breeding. Meanwhile, by selecting representative strains to carry out artificial inoculation identification of seedling blast and panicle blast, natural induction identification of a rice blast retransmission area and heat resistance identification, and by combining main agronomic trait evaluation in the whole growth period, a heat-resistant broad-spectrum durable rice blast-resistant breeding material is obtained. The breeding material bred by using the method disclosed by the invention has relatively strong heat resistance and broad-spectrum and lasting rice blast resistance, and the breeding process of heat-resistant and rice blast-resistant rice varieties is accelerated.

This invention provides an expression vector pHH40 and its application in the construction of engineered *C. truncatula* strains, belonging to the field of microbial engineering technology. pHH40, as an expression vector, can significantly improve the success rate of exogenous gene expression in *C. truncatula*. Furthermore, we used pHH40 to introduce the heat shock protein encoding gene lphsp into *C. truncatula*, and the resulting engineered *C. truncatula* strains can tolerate ethanol concentrations up to 14%, and can more quickly initiate and complete malolactic (MLF) fermentation in wine production, shortening the wine production cycle.

This invention discloses an integrated device and a method for rapid cultivation of aerobic granular sludge suitable for low-concentration domestic sewage, along with its application. The device comprises a batch reactor body integrating the following functional units: a full-area water distribution unit, including a main inlet pipe and distributed water distribution components; one end of the main inlet pipe is connected to the sewage source, and the other end extends to the bottom of the reactor cavity; the distributed water distribution components are connected to the main inlet pipe and equipped with multiple water distribution holes; a dual-zone aeration unit, including an air pump, a bottom aeration unit with a wide range of adjustable air volume, and a middle aeration unit with precise air volume control; a flow field optimization unit, located at the upper part of the reactor cavity and connected to the main inlet pipe; and a timing control unit, electrically connected to the full-area water distribution unit and the dual-zone aeration unit. The advantages of this invention are: by creating sedimentation selective pressure through dual-zone aeration to achieve sludge stratification, enhanced denitrification of heavy granular sludge, and removal of excess phosphorus from light flocculent sludge, it also has the advantages of shortening the cultivation cycle of aerobic granular sludge and highly efficient denitrification and phosphorus removal.

The application provides a chrysosporium inosporum breeding method and relates to the technical field of edible mushroom cultivation. The breeding method is as follows: the chrysosporium fruiting body is hung and cultured, and after the spores in the fruiting body are separated out, the spores are moved to a flat plate culture medium to culture the chrysosporium single colony, two chrysosporium single colonies are randomly picked and cross lines are drawn on the same clean flat plate culture medium to carry out hybridization culture, and the liquid strain of phanerochaete chrysosporium is simultaneously introduced as a mycelium growth auxiliary, is evenly smeared on the culture medium, and the ear is induced. The breeding method solves the technical problems of the existing cultivation method, such as complex operation, tedious steps, high requirement for equipment and long cultivation time, and realizes the technical effects of fast spore culture speed, simple operation, low equipment requirement and meeting the needs of factory production.

The application discloses a kind of in vitro directional induction lenticular lens organ by relying on stiffness controllable hydrogel and its construction method and application, technical scheme includes the following steps: (1) pluripotent stem cells are directionally induced and differentiated into primary ectoderm cell group;(2) prepare specific stiffness hydrogel and carry out surface cell adhesion treatment;(3) primary ectoderm cell is planted on the polyacrylamide hydrogel after washing and is induced into neural ectoderm cell group;(4) neural ectoderm cell is directionally differentiated into mature lenticular lens organ.The three-dimensional lenticular lens organ model obtained by the method has the top surface and the bottom surface of the spherical surface lenticular morphology, transparent and has capsule, lenticular lens epithelial cell, lenticular lens fiber cell and other structures, bottom surface is also completely wrapped by lenticular lens capsule.Compared with previous method, the formation of bottom surface complete capsule and spherical surface morphology is realized.