78 results about "Divinyl sulfone" patented technology

This invention relates to the production of polyclonal and monoclonal antibodies to specific sites of rapamycin (Sirolimus). The reactivity of these poly and monoclonal antibodies make them particularly useful for immunoassays for therapeutic drug monitoring (TDM). These immunoassays or TDM kits may include polyclonal or monoclonal antibodies to specific sites of rapamycin. These kits may also include various combinations of polyclonal antibodies, polyclonal and monoclonal antibodies or a panel of monoclonal antibodies. Rapamycin conjugate immunogens are prepared for the immunization of a host animal to produce antibodies directed against specific regions of the rapamycin molecule. By determining the specific binding region of particular antibody, immunoassays which are capable of distinguishing between the parent molecule, active metabolites, inactive metabolites and other structurally similar immunosuppressant compounds are developed. The use of divinyl sulfone (DVS) as the linker arm molecule for forming rapamycin-protein conjugate immunogens is described. DVS-linked rapamycin-protein conjugates were found to elicit antibodies with greater specificity to the rapamycin molecule than succinate linked conjugates.

The invention relates to a mixed gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and a preparation method of the mixed gel. The polylactic acid microspheres have the molecular weight of 15000-120000 and the average particle size of 10-150mu m, and the mass fraction of the polylactic acid microspheres in the mixed gel is 5-25%; a salt solution of the cross-linked hyaluronic acid gel is obtained by swelling and balancing the cross-linked hyaluronic acid gel in a sodium chloride solution or a phosphate buffer solution with the osmotic pressure of 250-350mOsmol/L and the pH value of 6.5-7.5; the cross-linked hyaluronic acid gel is a hyaluronic acid gel cross-linked by divinyl sulfone or glycidyl ether. A mixed gel product of polylactic acid and cross-linked hyaluronic acid, obtained by directly mixing the polylactic acid microspheres with the cross-linked hyaluronic acid gel obtained by swelling and balancing in the salt solution is uniform, fine, long in partial retention time, good in plasticity and few in side effects, has an obvious effect on eliminating wrinkles, and has few operation steps and stable product quality.

Disclosed are highly resilient and cohesive gels formed by the cross-linking of hyaluronan or hylan, their salts or derivatives thereof, using divinyl sulfone (DVS) as the cross-linking agent. Also disclosed are viscoelastic fluids containing alkylsulfone groups covalently attached to the backbone of the polymer, formed by the mono-functionalization of the cross-linking monomer DVS with hyaluronan and/or hylan. Mechanical properties such as values of hardness and cohesiveness are specified by the rheological properties of the gels. Also disclosed are methods for the preparation of such products. They have use in many applications as injectable and/or implantable devices and as drug delivery systems.

The invention relates to injection amphiphilic-microsphere-containing hyaluronic acid mixed gel and a preparation method thereof. The microsphere material relates to amphiphilic materials of PLLA-PEG, PLGA, PLGA-PEG and the like. The molecular weight of PLLA or PLGA is 10,000-500,000, the molecular weight of PEG is 1,000-10,000, and in PLGA, LA/GA=90/10-10/90. The average grain diameter of the microsphere is 1-200 [mu[m, and the mass percent of the microsphere in the gel is 1-50%; the gel is made from hyaluronic acid, divinyl sulphone crosslinked hyaluronic acid, or glycidyl ether crosslinked hyaluronic acid, the molecular weight of the hyaluronic acid is 100,000-3,000,000, and the mass percent of the hyaluronic acid is 1-50%. The gel can be also made from animal-derived collagen, chitosan, amino acid cellulose and sodium alginate. The microsphere mixed gel is prepared in a sodium chloride solution or a phosphate buffered solution with the osmotic pressure of 250-350 mOsm/L and pH of 6.5-7.5. According to the invention, the amphiphilic microsphere is directly mixed with hyaluronic acid gel, can be uniformly distributed in the gel easily, and is unlikely to aggregate into blocks in the water environment of a human body after being injected.

The invention discloses a mixed model expanded adsorbent bed medium and a preparation method thereof. In the composition of medium, matrix is a composite microsphere with fibrin/inorganic weighting agent, and petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin/inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off activation matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the mixed model expanded adsorbent bed medium taking the mercapto benzimidazole and the sulfonyl group as the petunidin. The novel expanded bed adsorbing medium developed by the invention combines with a plurality of petunidin-protein interaction mechanisms such as hydrophobic interaction, charge induction action, thiophilic action, etc, to form mixed model adsorption, which has the advantages of large adsorption capacity, high selectivity, etc., thus being used for high efficient separation and purification of bioactivators such as antibody, etc.

The invention provides a crosslinking hyaluronic acid gel and its preparation method. The method is characterized in that divinylsulfone is combined with polyethylene glycol for generating a novel cross-linking agent at first, the novel cross-linking agent is reacted with a hyaluronic acid molecule for producing a crosslinking hyaluronic acid gel. The crosslinking sodium hyaluronate gel obtained in the invention has good biological compatibility and longer half life period, and the particle is small and uniform, and is suitable for beautifying shaping, tissue filling, bone articular lubrication or medicine sustained-release preparation and the like.

The invention relates to a preparation method of vinyl sulfone derivatives. The vinyl sulfone derivatives have the structure disclosed as General Formula I. According to the preparation method, compounds i react with divinyl sulfone in the presence of a catalyst, wherein R is selected from H or C1-C8 alkyl; n is a whole number which is greater than or equal to 1; and the catalyst is selected from triaryl phosphine compounds or nitrogen heterocycle compounds. Under the action of the selected catalyst, the reaction can quickly occur under room temperature conditions to obtain the products. The method has the advantages of mild conditions, high reaction speed, high conversion rate and the like. The prepared products have the advantages of hydrolysis resistance, alkali resistance, broad reaction spectrum and the like, and have very high application value.

The invention discloses a nanometer polymer blocking agent for a water-based drilling fluid and a preparation method thereof. The blocking agent contains a large amount of nano particles. The preparation method comprises the following steps that 1, piperazine and diethylenetriamine are dissolved in water or an organic solvent, divinyl sulphone is added, the mole ratio of the divinyl sulphone to the piperazine to the diethylenetriamine is 2:1:1-3:1:1, N2 is continuously led into the water or the organic solvent to perform reaction at the temperature of 40-60 DEG C for 12-24 hours; 2, methyl alcohol is adopted to achieve product precipitate and washing, and vacuum drying is performed for 12-24 hours to obtain the nanometer polymer blocking agent. The blocking agent can effectively prevent drilling fluid filtrate from invading a stratum so as to prevent well wall collapse and other accidents, can be resistant to the temperature of 250 DEG C and is especially suitable for nanometer blocking of an ultra-high-temperature shale stratum of an ultra-deep well. The preparation method is reliable in principle, simple and convenient to operate and lower in cost, raw material sources are abundant, industrial production is easily achieved, and the nanometer polymer blocking agent has wide market prospect.

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin/inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin/inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantages of large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.

A non-aqueous electrolyte secondary battery including a positive electrode containing a positive active material, a negative electrode containing a negative active material and a non-aqueous electrolyte, characterized in that lithium transition metal complex oxide A formed by allowing LiCoO₂ to contain at least both of Zr and Mg and lithium transition metal complex oxide B having a layered structure and containing at least both of Mn and Ni as transition metals are mixed and used as said positive active material, and vinylene carbonate and divinyl sulfone are contained in said non-aqueous electrolyte.

Non-immunogenic biocompatible macromolecular sheet composition are formed from a cellulosic membrane, a binding moiety having a plurality of functional groups, and a glycosaminoglycan (GAG). The binding moiety has the formula of R1-X-R2 wherein R1 and R2 are the same or different. The binding moiety, through functional groups binds the cellulosic membrane with the glycosaminoglycan. R1 is covalently bound to a carbon or an oxygen of the cellulosic membrane. R2 is covalently bound to a carbon, an oxygen, or a nitrogen of the glycosaminoglycan. The binding moiety can be bis-oxyrane, butanediol-diglycidyl ether (BDE), or divinyl sulfone. The cellulosic membrane can be a cellulosic membrane, partially acetylated cellulose and a copolymer of hydroxyethyl-methacrylate with methyl methacrylate, abbreviated as HEMA-MMA. The non-immunogenic biocompatible macromolecular sheet composition can be formed into a pouch to encapsulate cells, tissues, pharmaceuticals, or biological metabolic products. In addition, the non-immunogenic biocompatible macromolecular sheet composition can also be used as a skin graft or a skin substitute. Further, these non-immunogenic biocompatible macromolecular sheet composition can also be used as a surface to culture cells in vitro.

The invention discloses a preparation method of composite crosslinking sodium hyaluronate gel microspheres for facial injection. The preparation method comprises the following steps: (1) preparing sodium hyaluronate into 10-30 percent g/ml sodium hyaluronate alkali liquor gel, adding an oil phase containing an emulsifier, carrying out emulsification at a high speed by a shearing machine to form an emulsion liquid of sodium hyaluronate microsphere particles, fully mixing, regulating a pH value to be 8-11 by using an alkaline solution, and standing to remove bubbles; (2) dissolving polyvinyl alcohol in hot water for injection with concentration to be 10-20 percent (m/v); (3) uniformly mixing the emulsion liquid of the sodium hyaluronate microsphere particles obtained in the first step and divinyl sulphone as a crosslinking agent according to the ratio of 2:1, standing to remove bubbles, and centrifugally removing the oil phase; (4) adding a polyvinyl alcohol solution obtained in the second step in the microsphere particle gel obtained in the third step according to the ratio of (1:4)-(4:1), mixing, and removing bubbles; and (5) washing the microsphere particles obtained in the fourth step by a soluble organic solvent, removing the residual oil phase, and regulating a pH value by the alkaline solution to be neutral.

Portland cement clinker LCMs that include Portland cement clinker to mitigate or prevent lost circulation in a well are provided. A Portland cement clinker LCM may include Portland cement clinker, Portland cement, a carrier fluid, and an inorganic consolidation activator. Another Portland cement clinker LCM may include Portland cement clinker and a crosslinked fluid, such as a polyuronide crosslinked via calcium ions or a polysaccharide crosslinked via divinyl sulfone. Yet another Portland cement clinker LCM may include Portland cement clinker and polymer fibers or particulate glass. Methods of lost circulation control using a Portland cement clinker LCM are also provided.

The invention provides a lithium-ion battery electrolyte and a lithium-ion battery containing the same. The electrolyte comprises a solvent and a lithium salt electrolyte, further comprises an addition agent LiFOB and an alkylene unsaturated compound of which the boiling point is between 180 DEG C and 250 DEG C, wherein the alkylene unsaturated compound is one or more of divinyl sulfone and dimethyl sulphoxide. The electrolyte provided by the invention can effectively improve high-temperature storage performance and cycle performance of the lithium-ion battery prepared by using the electrolyte.

The invention discloses an active dye printing process which includes the steps of printing, drying, roll alkali treatment, steaming fixation, washing, soaping and washing. In the printing process, dyes in printing paste are divinyl sulfone active dyes or 'double-active-group active dyes comprising a vinyl sulfone active group and a chorotriazine active group'. In roll alkali treatment, mangle expression is 30-50%. Adopted fixation alkali liquor comprises 5-15% of sodium carbonate, 5-10% of sodium silicate, 0.5-2% of sodium hydroxide and the balance water. In the printing process, alkali agents and urea added into the printing paste can be omitted, roll alkali treatment and steaming fixation are performed after printing and drying, urea is omitted in the process, ammonia nitrogen emissioncan be greatly reduced, and sewage discharge can reach standards. Besides, dye hydrolysis can be reduced, the stability of the color paste is prolonged, steaming time is shortened, steaming efficiencyis improved, and product production cost is reduced.

The invention discloses a thiophilic porous material, and a preparation method and application thereof, wherein the preparation method comprises the following step of mixing divinyl sulfone, N,N-methylene-bisacrylamide and first organic solvent under the condition of existence of an initiator, so that the thiophilic porous material is prepared. According to the design, the divinyl sulfone and the crosslinking agent of N,N-methylene-bisacrylamide take a copolymerization reaction under the initiation effect of a free radical reaction initiator to form a crossed linked framework structure and mutually penetrated through hole passages, so that the specificity mutual action can be generated with antibodies and compounds containing disulfide base groups under the existence of certain concentration of salts. Meanwhile, through the technical scheme, the operation of introducing sulfuryl into the thiophilic porous material by a double-bond polymerization method is provided for the first time; meanwhile, a one-kettle method is used for preparing the thiophilic porous material, and the simplicity, convenience and high speed of the preparation process are realized; the preparation method is simple; the effect of good repeatability is achieved.

The invention provides schizophyllan modified sodium hyaluronate microsphere gel, a preparation method of the sodium hyaluronate microsphere gel, and application of the sodium hyaluronate microspheregel in cosmetics. The method comprises the following steps: adding dried sodium hyaluronate powder into a hyaluronidase solution, and performing enzymolysis to obtain a sodium hyaluronate small-molecule solution; slowly adding the sodium hyaluronate small-molecule solution into a mixed oil phase, slowly stirring, adding a mixed cross-linking agent of calcium chloride and divinyl sulfone, cross-linking and curing to obtain sodium hyaluronate microspheres; adding the sodium hyaluronate microspheres and pre-treated schizophyllan into an alkaline solution containing polyethylene glycol, uniformlystirring, regulating the pH value of the system to be neutral, adding a phosphate buffer solution, standing and aging, thereby obtaining the schizophyllan modified sodium hyaluronate microsphere gel.The schizophyllan modified sodium hyaluronate microsphere gel prepared by the method disclosed by the invention is small in particle size, easily penetrates into the deep part of skin, can form tiny gel with protein-water, achieves the effect of deeply moisturizing for a long time, and has excellent moisture retention property.

The invention discloses a vinyl sulfone-functionalized polysulfone membrane and a preparation method and application thereof. Vinyl sulfone groups are contained on the surface of the vinyl sulfone-functionalized polysulfone membrane, and the preparation method comprises the steps: dissolving the polysulfone particles in an organic solvent, performing chloromethylation, performing a reaction between the obtained material with diethanolamine so as to introduce hydroxyl groups, and performing a reaction between the introduced product with divinyl sulfone under catalytic conditions so as to introduce vinyl sulfone functional groups on the surface of the membrane. Through the prepared vinyl sulfone-functionalized polysulfone membrane, further coupling of functional molecules can be achieved, the substrate has a wide application range, the reaction conditions are mild, the operation is simple, and environmental protection is achieved; and the vinyl sulfone-functionalized polysulfone membraneis a broad-spectrum polysulfone membrane which is a surface-functionalized substrate and has great potential.

A preparation method of a cross-linked sodium hyaluronate gel is disclosed, which including: preparing an alkaline aqueous solution of hyaluronic acid: formulating a sodium hyaluronate alkali liquor with the concentration of 10-30% g/ml; and carrying out a cross-linking reaction: the cross-linking agent used in the cross-linking reaction being divinyl sulfone or 1,4-butanediol diglycidyl ether, the cross-linking reaction being carried out in an alkaline aqueous solution of hyaluronic acid, the reaction temperature of the cross-linking reaction being 20-40° C., the time of the cross-linking reaction being 4-8 h, and the like. The method of this invention has many advantages, such as easily available raw materials, mild reaction conditions, high cross-linking efficiency, simple process and post-treatment, and easy operation. The obtained cross-linked sodium hyaluronate has a three-dimensional network structure by the crosslinking reaction with good mechanical properties, and can be used as a good drug carrier and a tissue engineering scaffold material.