80 results about "Divinyl sulfone" patented technology

Disclosed are highly resilient and cohesive gels formed by the cross-linking of hyaluronan or hylan, their salts or derivatives thereof, using divinyl sulfone (DVS) as the cross-linking agent. Also disclosed are viscoelastic fluids containing alkylsulfone groups covalently attached to the backbone of the polymer, formed by the mono-functionalization of the cross-linking monomer DVS with hyaluronan and / or hylan. Mechanical properties such as values of hardness and cohesiveness are specified by the rheological properties of the gels. Also disclosed are methods for the preparation of such products. They have use in many applications as injectable and / or implantable devices and as drug delivery systems.

The invention relates to a preparation technique of crosslinked hyaluronic acid gel preparation, characterized in that divinylsulphone reacts with hydroxyl group of hyaluronic acid to form ether linkage. The steps are that the raw material of hyaluronic acid being put into container and dissolving in alkali reagent; stirring to make it thoroughly dissolve; mixing the alkaline hyaluronic acid solution and divinylsulphone in container to form crosslinked hyaluronic acid gel clot; adding substance riched in hydroxyl groups into the reaction solution; extracting the crosslinked hyaluronic acid gel clot and putting it into agitator to stir; and then crosslinked hyaluronic acid gel becoming micro grained. The advantages thereof are that the gel preparation is suitable for injection in vivo, with long residence timen in humans and good filling effect.

The invention relates to a hyaluronic acid mixed gel containing amphiphilic microspheres for injection and a preparation method thereof. The microsphere material involves amphiphilic materials such as PLLA-PEG, PLGA, and PLGA-PEG. The molecular weight of PLLA or PLGA is 10,000-500,000, and that of PEG is 1,000-10,000. In PLGA, LA / GA=90 / 10-10 / 90. The average particle size of the microspheres is 1-200 μm, and the mass fraction in the gel is 1-50%; the gel component is hyaluronic acid, or hyaluronic acid cross-linked with divinyl sulfone or glycidyl ether, The molecular weight of hyaluronic acid is 100,000-3,000,000, and the mass fraction is 1-50%. The gel component can also be animal-derived collagen, chitosan, amino acid cellulose, sodium alginate, and the like. The microsphere mixed gel is prepared in a sodium chloride solution or a phosphate buffer solution with an osmotic pressure of 250-350 mOsm / L and a pH of 6.5-7.5. In the present invention, the amphiphilic microspheres are directly mixed with the hyaluronic acid gel, and the amphiphilic microspheres are easily and uniformly distributed in the gel, and after being injected into the human body, they are not easy to assemble into lumps in the water environment of the human body.

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantagesof large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.