Thiophilic porous material, and preparation method and application thereof

A technology of porous materials and organic solvents, applied in the field of preparation of affinity sulfur-loving materials, can solve the problems of high preparation cost, inability to withstand high pressure, low mechanical strength, etc., and achieve simple preparation method, simple and convenient preparation process, and good repeatability Effect

Inactive Publication Date: 2016-05-04
ANHUI NORMAL UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] In view of the above-mentioned prior art, the purpose of the present invention is to overcome the poor repeatability of the affinity thiophilic material in the prior art, which cannot withstand high pressure, has low mechanical strength, high preparation cost, difficult storage, and cannot be reused many times, which cannot meet the requirements of Qualcomm. A large amount of antibody purification problem, so as to provide a simple preparation method, safe preparation process, good repeatability, and low price, greatly reduce production costs, high stability, and reusable, greatly reduce the cost of use, improve the scope of use Thiophilic porous material and preparation method thereof

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  • Thiophilic porous material, and preparation method and application thereof
  • Thiophilic porous material, and preparation method and application thereof
  • Thiophilic porous material, and preparation method and application thereof

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preparation example Construction

[0020] The invention provides a method for preparing a sulfur-loving porous material, wherein the preparation method comprises: in the presence of an initiator, divinyl sulfone, N,N-methylenebisacrylamide and a first organic solvent mixed to obtain a thiophilic porous material. Its preparation principle is as figure 1 shown.

[0021] The above design forms a cross-linked skeleton structure and interpenetrating channels through the copolymerization of divinyl sulfone and the cross-linking agent N,N-methylenebisacrylamide under the initiation of a free radical reaction initiator, so that in a certain In the presence of concentrated salt, it can specifically interact with compounds containing disulfide groups and antibodies, thereby achieving separation and purification. At the same time, through the above technical scheme, it is proposed for the first time to introduce sulfone groups into thiophilic porous materials by the method of double bond polymerization. Good repeatabil...

Embodiment 1

[0043] Dissolve 12.5mg of divinyl sulfone and 30.0mg of N,N-methylenebisacrylamide in 85.0μL of dimethyl sulfoxide and place in a centrifuge tube, then add 85.0μL of dodecanol and 1.0mg of azobis isobutyronitrile, then placed in a vortex oscillator for full dissolution, and then ultrasonically degassed for 20.0 min to obtain a uniformly mixed solution, then seal both ends of the centrifuge tube with silicone rubber, and place the sealed centrifuge tube Put it in a water bath at a temperature of 85.0°C for 10.0 hours to prepare the mixture M1; take out the prepared mixture M1 and cut it into small pieces, then take 3.0 g of the mixture M1 and put it in a Soxhlet extractor, add methanol, and place in The mixture M2 was obtained by extracting at 110.0°C for 24.0 hours; the mixture M2 was dried in a vacuum oven at a temperature of 100.0°C for 12.0 hours to obtain a sulfur-philic porous material A1.

Embodiment 2

[0045] 1) Rinse the empty capillary column with a diameter of 75.0 μm with 0.1mol / L NaOH solution for 30.0min, then rinse the capillary with deionized water for 30.0min, then rinse the capillary with 0.1mol / L HCl solution for 30.0.min, and then use Rinse the capillary with ion water until the pH value of the effluent liquid is 7.0, then rinse the capillary with methanol solution for 30.0min, and blow dry with nitrogen; then inject a mixture of methanol and 3-(methacryloyloxy)propyltrimethoxysilane into the capillary (Wherein, relative to 1.3 parts by weight of 3-(methacryloyloxy)propyltrimethoxysilane, the amount of methanol used is 1 part by weight) and then reacted at a temperature of 50.0°C for 12.0h; Rinse, and finally dry the above-mentioned pretreated capillary with nitrogen for use;

[0046] 2) Dissolve 12.5mg of divinylsulfone and 30.0mg of N,N-methylenebisacrylamide in 85.0μL of dimethyl sulfoxide, then add 85.0μL of dodecanol and 1.0mg of azobisisobutyronitrile, The...

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Abstract

The invention discloses a thiophilic porous material, and a preparation method and application thereof, wherein the preparation method comprises the following step of mixing divinyl sulfone, N,N-methylene-bisacrylamide and first organic solvent under the condition of existence of an initiator, so that the thiophilic porous material is prepared. According to the design, the divinyl sulfone and the crosslinking agent of N,N-methylene-bisacrylamide take a copolymerization reaction under the initiation effect of a free radical reaction initiator to form a crossed linked framework structure and mutually penetrated through hole passages, so that the specificity mutual action can be generated with antibodies and compounds containing disulfide base groups under the existence of certain concentration of salts. Meanwhile, through the technical scheme, the operation of introducing sulfuryl into the thiophilic porous material by a double-bond polymerization method is provided for the first time; meanwhile, a one-kettle method is used for preparing the thiophilic porous material, and the simplicity, convenience and high speed of the preparation process are realized; the preparation method is simple; the effect of good repeatability is achieved.

Description

technical field [0001] The invention relates to a preparation method of an affinity thiophile material, in particular to a preparation method of a thiophile porous material. Background technique [0002] Due to the rapid development of immunology, genetic engineering, and molecular biology, antibodies have been widely used in biotechnology, disease prevention, diagnosis and treatment, biopharmaceuticals, and medical care, and their demand has shown an exponential growth trend year by year. Clinical disease treatment and pharmacological research also put forward high requirements on the purity, cost, and activity of antibodies (see "Methods" 2012, No. 56, pp. 116-129). However, traditional antibody separation methods, such as ion exchange and hydrophobic chromatography, have low separation efficiency, complicated operation, long separation cycle, high production cost, and large loss of biological activity, making it difficult to achieve large-scale industrial application. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F228/02C08F222/38
CPCC08F228/02C08F222/38
Inventor 刘云春诸林
Owner ANHUI NORMAL UNIV
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