56 results about "Expanded bed adsorption" patented technology

The present invention provides an extracorporeal adsorption method for removing harmful substances from blood in a way that is practicable in everyday clinical practice and applicable for the timely intervention to present the development of sepsis. Said extracorporeal adsorption method being effected by an adsorption column assembly where the adsorption column assembly comprising a column and an adsorption medium in the form of particles. The sedimented volume of said particles being at the most 80% of the volume of the column.

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin/inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin/inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantages of large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.

The invention belongs to a coupling device and a coupling technology for in situ extraction of succinic acid by an expanded bed and fermentation and separation of the succinic acid, in particular relates to a technology for producing the succinic acid by the fermentation method, a technology for separating the succinic acid by the expanded bed and a fermentation and separation coupling process. The device is to couple a chromatography column of the expanded bed and a succinic acid bioreactor, so as to extract the succinic acid in situ. The device utilizes the characteristic of the chromatography column of the expanded bed that microbial zymotic liquid provided with solid materials is allowed to directly enter the chromatography column, avoids the blockage problem of a chromatography column of a fixed bed due to direct coupling of the fixed bed and the bioreactor in the prior art, saves an additional membrane separator between the bioreactor and the chromatography column of the expanded bed, realizes direct coupling of the chromatography column of the expanded bed and the bioreactor, and performs in situ extraction of target substances and does not cause blockage of the chromatography column of the expanded bed. Moreover, a coupling system for in situ extraction of the succinic acid by the expanded bed and the fermentation and the separation of the succinic acid is established to meet the requirements of sterilization and pollution-free operation.

The present invention provides industrial scale expanded bed adsorption process for fractionation and isolation of bio-molecules from fluids, preferably proteins from milk and whey, in a cost-effective manner. This is accomplished by operating the expanded bed column at high temperatures of at last 40° C., combined with applying flow rates greater than 1.500 cm/hour.

The invention provides a polyamine-modified chitosan base expanded bed adsorbing medium and a preparation method of the polyamine-modified chitosan base expanded bed adsorbing medium, and relates to an adsorbing medium and a preparation method of the adsorbing medium. The matrix is a chitosan/stainless steel powder composite micro-ball, and the genin is induced phenyl by activating benzaldehyde and the re-coupled polyethylene polyamine by activating epichlorohydrin. The preparation method comprises the following steps of: adopting a reversed phase suspension crosslinking method to prepare the chitosan/stainless steel powder composite micro-ball which is taken as a blank matrix; and adopting a method that two genins are designed in a dividing way to firstly react chitosan unit molecule C2 site amino in the micro-ball matrix with benzaldehyde, build the hydrophobic interacted genin, activate matrix chitosan unit molecule C6 site hydroxyl by epichlorohydrin, and couple polyethylene polyamine, so as to obtain the expanded bed adsorbing medium which takes the phenyl and the polyethylene polyamine as the genins. The expanded bed adsorbing medium provided by the invention has the ion exchange genin and the hydrophobic genin, and the absorbing and separating efficiency and the selectivity of target matter in feed liquid can be improved, so that the biological substance such as the protein and the like can be separated and purified on a large scale.

The invention relates to a method for preparing composite TiO2 copolymer microspheres and application of the composite TiO2 copolymer microspheres in natural product separation and purification. According to the method, an oil phase containing surface-modified TiO2, benzoyl peroxide serving as an initiator, GMA serving as a monomer, DVB serving as a crosslinking agent and the oil phase of a pore forming agent is added into a composite aqueous phase containing gelatin and sodium chloride, and the mixture is heated to cure to form microspheres; and beta-CD is directly immobilized on the microspheres serving as a immobilization matrix in the presence of sodium hydride to form the composite TiO2 copolymer microspheres. The copolymer microspheres can be used as an expanded bed adsorption medium to separate isoflavones from soybean waste molasses and purify the isoflavones. The method has the advantages of simple preparation process and easy control and amplification. The prepared TiO2 copolymer microspheres have the advantages of good medium sphericity, mild elution conditions, high biocompatibility, excellent hydrophilicity and wide application prospect in the natural product separation and purification.

The invention discloses an expanded bed adsorption matrix and a preparation method thereof. The preparation method comprises the following steps: 1) dissolving acrylamide, a crosslinking agent and a water-soluble gelatinized starch colloid liquid in deionized water; 2) adding inorganic weighting agent particles into the mixed solution, and uniformly dispersing; 3) adding an initiator into the uniformly dispersed solution, adding the solution into a dispersing-agent-dissolved continuous phase (oil phase) to constitute a reversed phase suspension disperse system; 4) heating the reversed phase suspension disperse system to carry out polymerization reaction; 5) after the reaction is finished, removing the continuous phase, cleaning, and adding amylase to remove the water-soluble starch, thereby obtaining composite microspheres; and 6) filtering the composite microspheres, washing, and carrying out wet screening to finally obtain the expanded bed matrix using polyacrylamide as the base material. The expanded bed matrix has the advantages of favorable sphericity, higher density, larger pore size, reasonable particle size distribution, favorable mechanical strength, favorable biocompatibility, stable properties, low cost and the like.

The invention relates to a composite GMA (Glycidyl Methacrylate)/ZrO2 copolymer microsphere as well as preparation and application thereof. In the microsphere, the grain size is 120 to 400mu m, the core component is ZrO2, and the shell components are GMA and DVB (Divinylbenzene); and the microsphere contains 0.5 to 14 percent by mass of ZrO2 and 50 to 70 percent by mass of GMA and the balance of DVB. The preparation method of the composite GMA/ZrO2 copolymer microsphere comprises the following steps of: mixing a polymerization phase containing GMA, DVB, surface-modified ZrO2, BPO (Benzoyl Peroxide), toluene and n-heptane with a dispersed-phase aqueous solution containing gelatin and sodium chloride, and heating and solidifying to form a microsphere; then immobilizing Beta-CD (Cyclodextrin) under the existence of NaH, KI and Bu4NBr by taking the microsphere as a vector to obtain the composite GMA/ZrO2 copolymer microsphere. The microsphere has regular sphere, high density, acid-base resistance and good hydrophilcity and can be applied to separating and purifying granatum polyphenol from a granatum extract as an expanded bed adsorption matrix.

The present invention provides the process of purifying hematoglobin. Expanding bed adsorption technology is adopted, and through material feeding from bottom to top and chromatographic medium expanding, cell fragment, hybrid protein and other impurities are made to pass through the bed layer and hematoglobin is adsorbed by the medium, so as to realize direct purification of hematoglobin from disintegrated human or animal blood cell liquid. Without centrifugation or filtering to eliminating segment, the product reach electrophoresis pure level of hematoglobin in short time, high yield and less loss in product activity. The present invention is suitable for industrial production of natural hemotoglobin.

A separation column for expanded bed adsorption comprises a column tube (2), a base (15) carrying an inlet rotor structure (6) for pumping in process liquid, and a top cap (3). The top cap (3) is conical in form, and has a peripheral flange 31 by which it is rigidly fixed to the top edge flange (22) of the column tube (2). The angle of the convergent interior surface (35) of the conical top cap (3) may be between 10 and 25°. A vortex-inhibitor component (8) projects down below the outlet structure (4) at the top of the cap, projecting into the operating space (15) of the column to inhibit rotation of liquid in the column interior. An expanded bed adsorption process is done with upward flow of liquid in the column through a bed of media particles.

Chromatographic column system, in particular a chromatographic column system of the expanded bed adsorption column type comprises a column connected to a fluid inlet and a fluid outlet and dimensioned to comprise an expandable filter bed between said fluid inlet and said fluid outlet. A top collector is adapted for collecting substance filtered by said filter bed and top collector actuator is provided for actuation of the top collector. A top stratum position detector is constructed and adapted to detect a top stratum of the filter bed when in expanded state; and a controller is provided communicatively coupled to said top stratum position detector and said top collector actuator, for moving the top collector as a function of a detected top stratum position. A clean and automated way is provided to optimize the collection of filtered substance.

The invention provides a method for preparing fibrinogen, which comprises the following steps: 1) by using an agarose tungsten carbide-DEAE (diethylaminoethanol) ion exchange medium as a column chromatography filler, preparing an expanded bed action chromatographic column; 2) passing plasma through the chromatographic column in the step 1) to perform primary purification, and collecting a fibrinogen initial product; 3) carrying out acid precipitation on the fibrinogen initial product obtained in the step 2) to remove impurities; 4) removing viruses from the impurity-removed fibrinogen obtained in the step 3); and 5) further purifying the virus-removed fibrinogen in the step 4) by using MacroCap Q column chromatography to obtain the target fibrinogen solution. The method has the advantages of higher technical continuity and higher degree of automation, greatly reduces the residual chemical substances, and enhances the safety and quality of the product.

The invention discloses a constraint bed adsorption separation technology. The constraint bed adsorption separation technology is mainly characterized in that an adsorbent is constrained on a fixed framework by utilizing an external force, gaps among a column body, the fixed framework, the adsorbent or a 'constraint assembly' constrained by an 'aggregate' allow solid particles to pass through, and as long as the flow velocity of fluid does not exceed a 'constraint force', the adsorbent can keep an 'initial constraint state' and is regularly arranged in a column of a constraint bed respectively according to three modes, namely hard constraint, soft constraint and aggregate constraint. The constraint bed adsorption separation technology can be used for directly extracting target ingredients from particle materials just like expanded bed adsorption to obtain a separation effect similar to that of a fixed bed, and also avoids the special conditions such as the adsorbent with certain density and particle size distribution, a fluid distributor system and specific flow velocity of the fluid necessary for an expanded bed; an expansion process is very convenient, low in cost and convenient to operate, and the constraint bed adsorption separation technology can be used for a material separation process, and especially can be used for a reaction process.

The method for producing recombinant human epidermal growth factor by using genetically-engineered colibacillus includes the following steps: engineering bacterium fermentation: activating stored colibacillus by means of culture medium, fermenting main medium and introducing the human epidermal growth factor into fermentation cutlure liquor; expanding bed adsorption; centrifugalizing fermentationculture liquor, adding supernatant fluid into the expanding bed to make adsorption and collecting elution peak containing human epidermal growth factor; gel separation; adding collected eluent into gel chromatographic column, separating and collecting human epidermal growth factor elution peak; freezing, sublimating and drying; freezing collected liquor at -40 deg.C, sublimating and drying so as to obtain the invented product.

The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspension from step (iii) to an expanded bed adsorption process obtaining the purified protein product.

The invention relates to a method of producing gentamicin B with gentamicin B fermentation liquid. The method includes the steps of: 1) regulating pH value of the gentamicin B fermentation liquid to 3-5 with phosphoric acid, fully stirring the fermentation liquid, adding CaO to regulate the pH of the fermentation liquid to 5-6, fully stirring the fermentation liquid, successively adding a heavy metal precipitant and a co-filtering agent to treat the fermentation liquid, and performing plate-and-frame filtration and chloroform layering to obtain a gentamicin B water solution; 2) performing ceramic membrane filtration, expanded bed adsorption, crystallization and drying to produce a gentamicin B pure product. The method achieves stable and high-effective production of the gentamicin B, increases extraction yield and effective content of the gentamicin B, is simple in processes and low in production cost, reduces environmental pollution index and reduces environmental protection treatmentcost.

The invention discloses a method for adsorbing and separating human serum albumin by expanded bed adsorption based on a mixed mode, which can directly separate and recombine human serum albumin from a yeast fermentation solution, and belongs to the protein separation technology in the biochemical field. The method comprises the following steps: (1) preprocessing a fermentation solution; obtaining the yeast fermentation solution containing the human serum albumin, adding sodium caprylate, and heating and deactivating protease; (2) performing expanded bed adsorption, directly separating the yeast fermentation solution by using an expanded bed filled with a mixed-mode adsorption agent, and collecting elution peaks; and (3) desalting and drying: desalting a collected solution, freeze drying to obtain the human serum albumin with the purity greater than 95 percent. The invention is characterized by developing a novel separation process, so that the high-purity recombined human serum albumin can be directly separated from the yeast fermentation solution. A critical point of the invention lies in adopting the expanded bed adsorption agent based on the mixed mode, cells do not need to be removed from the yeast fermentation solution, the ion strength does not need to be adjusted, the elution condition is moderate, and the method has the characteristics of simple process, high separation efficiency and high yield.