56 results about "Expanded bed adsorption" patented technology

The present invention provides an extracorporeal adsorption method for removing harmful substances from blood in a way that is practicable in everyday clinical practice and applicable for the timely intervention to present the development of sepsis. Said extracorporeal adsorption method being effected by an adsorption column assembly where the adsorption column assembly comprising a column and an adsorption medium in the form of particles. The sedimented volume of said particles being at the most 80% of the volume of the column.

The invention discloses an expanded bed adsorption medium for separating antibodies by taking mercapto ehtylpyridine and sulfonyl groups as petunidins, and a preparation method. In the composition of the medium, interstitial substance is cellulose / inorganic weighting-agent composite microspheres, and the petunidins are sulfonyl groups or mercapto ehtylpyridine. The cellulose / inorganic weighting-agent composite microspheres which are used as the interstitial substance are obtained by adopting an opposite-phase suspension thermal-reproduction method; dried activation interstitial substance, the mercapto ehtylpyridine and sodium hydroxide containing ammonium persulfate are mixed for coupling to obtain the expanded bed adsorption medium for separating the antibodies by taking the mercapto ehtylpyridine and the sulfonyl groups as the petunidins. The novel expanded bed adsorption medium developed in the invention enhances the selectivity of mercapto ehtylpyridine petunidin to the antibodies through bring in the sulfonyl groups, has good adsorption separation selectivity to the antibodies, and can be used for the large-scale preparation of the antibodies.

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantages of large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.

The invention belongs to a coupling device and a coupling technology for in situ extraction of succinic acid by an expanded bed and fermentation and separation of the succinic acid, in particular relates to a technology for producing the succinic acid by the fermentation method, a technology for separating the succinic acid by the expanded bed and a fermentation and separation coupling process. The device is to couple a chromatography column of the expanded bed and a succinic acid bioreactor, so as to extract the succinic acid in situ. The device utilizes the characteristic of the chromatography column of the expanded bed that microbial zymotic liquid provided with solid materials is allowed to directly enter the chromatography column, avoids the blockage problem of a chromatography column of a fixed bed due to direct coupling of the fixed bed and the bioreactor in the prior art, saves an additional membrane separator between the bioreactor and the chromatography column of the expanded bed, realizes direct coupling of the chromatography column of the expanded bed and the bioreactor, and performs in situ extraction of target substances and does not cause blockage of the chromatography column of the expanded bed. Moreover, a coupling system for in situ extraction of the succinic acid by the expanded bed and the fermentation and the separation of the succinic acid is established to meet the requirements of sterilization and pollution-free operation.

The present invention discloses the technology of preparing gel carrier microballoon with loaded inert grain. The preparation process includes preparing suspension, emulsifying and coating reaction, and curing and crosslinking process. The preparation process features that the glass bead is dispersed inside the water solution of agarose to prepare suspension; the suspension is then added into salad oil while adding emulsifier Span-80 and stirring for emulsification and coating reaction to lower temperature fast to form microballoon; and epoxy chloropropane is then added for crosslinking at alkaline condition and sodium borohydride is added for reduction to prepare dispersed medium with particle size 40-500 mincrons and agerose content 20-70 wt%. The dispersed medium exhibits certain density and particle size distribution and has high density in expanding bed, column efficiency, adsorption capacity and thus wide application.

The invention provides a method for purifying lactoferrin from milk serum by using an expanded bed adsorption technology. The method comprises the following steps of: (1) adding water into sweet whey powder for dissolving into a whey solution or adjusting the pH of commercially available liquid whey by using NaOH; (2) filling a cation exchanging chromatography medium into an expanded bed, making a phosphate buffer solution pass through a bed layer from bottom to top, and balancing the expanded bed; (3) introducing the solution obtained in the step (1) for adsorbing, eluting by using a buffer solution containing NaCl, and collecting an eluent; and (4) cleaning by using NaOH, and regenerating a medium by using a buffer solution. In the method, expanded bed adsorption is taken as a core separating method, the purity of lactoferrin obtained by one-step separation is up to 88-93 percent, the recovery ratio is 47-86 percent, a process is easy, convenient and efficient, the equipment investment is small, and the method is suitable for industrial production.

The present invention provides industrial scale expanded bed adsorption process for fractionation and isolation of bio-molecules from fluids, preferably proteins from milk and whey, in a cost-effective manner. This is accomplished by operating the expanded bed column at high temperatures of at last 40° C., combined with applying flow rates greater than 1.500 cm / hour.

The invention discloses a novel bioseparation material and a preparation method thereof. The preparation method comprises the following steps: processing hydrophilic gel microspheres in a sym-triazine activator as a matrix to prepare an activated matrix, and bonding the activated matrix with two micro-molecular ligands comprising 4-(2-hydroxyethyl)-pyridine and the like through a coupling process to obtain the medium having a high specificity and a good affinity selectivity. The separation medium is used for the industrialized large-scale antibody production through cooperating with affinity chromatography chromatogram or expanded bed adsorption, is highly cost-competitive, allows highly pure antibody products to be directly extracted from biological raw materials comprising blood, serum, blood plasma, blood plasma fractions, ascites, cell culturing solutions, cell homogenates, milk liquids, colostrum and the like only through one step, and realizes time saving and convenience.

The invention discloses a preparation method using Escherichia coli to express oligopeptide-1. The method comprises the following steps: 1, carrying out engineering bacteria fermentation: picking single colony from a preserved strain tube, inoculating a culture medium with the single colony, adding ampicillin, and carrying out shaking culture; 2, carrying out expanded bed adsorption: centrifuging a fermentation liquid by using a refrigerated centrifuge, removing the obtained precipitate, reserving the fermentation liquid, adding the fermentation liquid to an expanded bed, eluting, collecting a human oligopeptide-1-containing eluate at the elution peak, and preserving the eluate for later use; 3, carrying out gel separation: adding the eluate to a gel chromatography column through a chromatographic system, eluting with a buffer solution, and reserving the eluate obtained at an absorption peak; and 4, freeze-drying the eluate to obtain oligopeptide-1. The method has the advantages of simplicity, low cost and high yield. The prepared oligopeptide-1 can be used in drugs, healthcare foods and cosmetics as an active component or additive.

The invention provides a polyamine-modified chitosan base expanded bed adsorbing medium and a preparation method of the polyamine-modified chitosan base expanded bed adsorbing medium, and relates to an adsorbing medium and a preparation method of the adsorbing medium. The matrix is a chitosan / stainless steel powder composite micro-ball, and the genin is induced phenyl by activating benzaldehyde and the re-coupled polyethylene polyamine by activating epichlorohydrin. The preparation method comprises the following steps of: adopting a reversed phase suspension crosslinking method to prepare the chitosan / stainless steel powder composite micro-ball which is taken as a blank matrix; and adopting a method that two genins are designed in a dividing way to firstly react chitosan unit molecule C2 site amino in the micro-ball matrix with benzaldehyde, build the hydrophobic interacted genin, activate matrix chitosan unit molecule C6 site hydroxyl by epichlorohydrin, and couple polyethylene polyamine, so as to obtain the expanded bed adsorbing medium which takes the phenyl and the polyethylene polyamine as the genins. The expanded bed adsorbing medium provided by the invention has the ion exchange genin and the hydrophobic genin, and the absorbing and separating efficiency and the selectivity of target matter in feed liquid can be improved, so that the biological substance such as the protein and the like can be separated and purified on a large scale.

The invention relates to a method for preparing composite TiO2 copolymer microspheres and application of the composite TiO2 copolymer microspheres in natural product separation and purification. According to the method, an oil phase containing surface-modified TiO2, benzoyl peroxide serving as an initiator, GMA serving as a monomer, DVB serving as a crosslinking agent and the oil phase of a pore forming agent is added into a composite aqueous phase containing gelatin and sodium chloride, and the mixture is heated to cure to form microspheres; and beta-CD is directly immobilized on the microspheres serving as a immobilization matrix in the presence of sodium hydride to form the composite TiO2 copolymer microspheres. The copolymer microspheres can be used as an expanded bed adsorption medium to separate isoflavones from soybean waste molasses and purify the isoflavones. The method has the advantages of simple preparation process and easy control and amplification. The prepared TiO2 copolymer microspheres have the advantages of good medium sphericity, mild elution conditions, high biocompatibility, excellent hydrophilicity and wide application prospect in the natural product separation and purification.

The invention discloses an expanded bed adsorption matrix and a preparation method thereof. The preparation method comprises the following steps: 1) dissolving acrylamide, a crosslinking agent and a water-soluble gelatinized starch colloid liquid in deionized water; 2) adding inorganic weighting agent particles into the mixed solution, and uniformly dispersing; 3) adding an initiator into the uniformly dispersed solution, adding the solution into a dispersing-agent-dissolved continuous phase (oil phase) to constitute a reversed phase suspension disperse system; 4) heating the reversed phase suspension disperse system to carry out polymerization reaction; 5) after the reaction is finished, removing the continuous phase, cleaning, and adding amylase to remove the water-soluble starch, thereby obtaining composite microspheres; and 6) filtering the composite microspheres, washing, and carrying out wet screening to finally obtain the expanded bed matrix using polyacrylamide as the base material. The expanded bed matrix has the advantages of favorable sphericity, higher density, larger pore size, reasonable particle size distribution, favorable mechanical strength, favorable biocompatibility, stable properties, low cost and the like.

The invention relates to a composite GMA (Glycidyl Methacrylate) / ZrO2 copolymer microsphere as well as preparation and application thereof. In the microsphere, the grain size is 120 to 400mu m, the core component is ZrO2, and the shell components are GMA and DVB (Divinylbenzene); and the microsphere contains 0.5 to 14 percent by mass of ZrO2 and 50 to 70 percent by mass of GMA and the balance of DVB. The preparation method of the composite GMA / ZrO2 copolymer microsphere comprises the following steps of: mixing a polymerization phase containing GMA, DVB, surface-modified ZrO2, BPO (Benzoyl Peroxide), toluene and n-heptane with a dispersed-phase aqueous solution containing gelatin and sodium chloride, and heating and solidifying to form a microsphere; then immobilizing Beta-CD (Cyclodextrin) under the existence of NaH, KI and Bu4NBr by taking the microsphere as a vector to obtain the composite GMA / ZrO2 copolymer microsphere. The microsphere has regular sphere, high density, acid-base resistance and good hydrophilcity and can be applied to separating and purifying granatum polyphenol from a granatum extract as an expanded bed adsorption matrix.

The invention discloses a preparation method of human epidermal growth factor (EGF). The method comprises the following steps: fermenting with engineering bacteria, picking up a single colony from a preserved bacteria tube to inoculate in seed culture medium, adding Ampicillin, culturing with a shaker; performing expanded bed adsorption, using a refrigeration centrifuge to centrifuge the fermentation solution, discarding fermentation liquid to reserve precipitate, performing ultrasonic processing, centrifuging, adding the product in an expanded bed, eluting, collecting the elution peak with EGF, reserving the elution solution to store for standby; performing gel separation, using a chromatography system to add the elution solution in a gel chromatographic column, buffering the elution solution, reserving the absorption peak; and adopting the sublimation freezing-drying technology to obtain the finished product. The invention has the advantages of simple method, high product concentration, low operating cost, high yield and simple extraction technology.

The present invention provides the process of purifying hematoglobin. Expanding bed adsorption technology is adopted, and through material feeding from bottom to top and chromatographic medium expanding, cell fragment, hybrid protein and other impurities are made to pass through the bed layer and hematoglobin is adsorbed by the medium, so as to realize direct purification of hematoglobin from disintegrated human or animal blood cell liquid. Without centrifugation or filtering to eliminating segment, the product reach electrophoresis pure level of hematoglobin in short time, high yield and less loss in product activity. The present invention is suitable for industrial production of natural hemotoglobin.

The invention relates to a process for separation and purification of gene recombinant protein heme oxygenase, which comprises subjecting the expression recombination protoheme oxygenase-1 fermentation liquor to centrifugal collection of cells, ultrasonic wave fragmenting, expanded-bed ion exchange chromatography and gel filtration chromatography for purification preparation. Compared with the existing separation process, provided method has the advantages of substantially reduced time of separation process and increased yield of recombination human heme oxygenase-1.

A separation column for expanded bed adsorption comprises a column tube (2), a base (15) carrying an inlet rotor structure (6) for pumping in process liquid, and a top cap (3). The top cap (3) is conical in form, and has a peripheral flange 31 by which it is rigidly fixed to the top edge flange (22) of the column tube (2). The angle of the convergent interior surface (35) of the conical top cap (3) may be between 10 and 25°. A vortex-inhibitor component (8) projects down below the outlet structure (4) at the top of the cap, projecting into the operating space (15) of the column to inhibit rotation of liquid in the column interior. An expanded bed adsorption process is done with upward flow of liquid in the column through a bed of media particles.

Chromatographic column system, in particular a chromatographic column system of the expanded bed adsorption column type comprises a column connected to a fluid inlet and a fluid outlet and dimensioned to comprise an expandable filter bed between said fluid inlet and said fluid outlet. A top collector is adapted for collecting substance filtered by said filter bed and top collector actuator is provided for actuation of the top collector. A top stratum position detector is constructed and adapted to detect a top stratum of the filter bed when in expanded state; and a controller is provided communicatively coupled to said top stratum position detector and said top collector actuator, for moving the top collector as a function of a detected top stratum position. A clean and automated way is provided to optimize the collection of filtered substance.

The invention provides a method for preparing fibrinogen, which comprises the following steps: 1) by using an agarose tungsten carbide-DEAE (diethylaminoethanol) ion exchange medium as a column chromatography filler, preparing an expanded bed action chromatographic column; 2) passing plasma through the chromatographic column in the step 1) to perform primary purification, and collecting a fibrinogen initial product; 3) carrying out acid precipitation on the fibrinogen initial product obtained in the step 2) to remove impurities; 4) removing viruses from the impurity-removed fibrinogen obtained in the step 3); and 5) further purifying the virus-removed fibrinogen in the step 4) by using MacroCap Q column chromatography to obtain the target fibrinogen solution. The method has the advantages of higher technical continuity and higher degree of automation, greatly reduces the residual chemical substances, and enhances the safety and quality of the product.

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantagesof large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.

The invention discloses a constraint bed adsorption separation technology. The constraint bed adsorption separation technology is mainly characterized in that an adsorbent is constrained on a fixed framework by utilizing an external force, gaps among a column body, the fixed framework, the adsorbent or a 'constraint assembly' constrained by an 'aggregate' allow solid particles to pass through, and as long as the flow velocity of fluid does not exceed a 'constraint force', the adsorbent can keep an 'initial constraint state' and is regularly arranged in a column of a constraint bed respectively according to three modes, namely hard constraint, soft constraint and aggregate constraint. The constraint bed adsorption separation technology can be used for directly extracting target ingredients from particle materials just like expanded bed adsorption to obtain a separation effect similar to that of a fixed bed, and also avoids the special conditions such as the adsorbent with certain density and particle size distribution, a fluid distributor system and specific flow velocity of the fluid necessary for an expanded bed; an expansion process is very convenient, low in cost and convenient to operate, and the constraint bed adsorption separation technology can be used for a material separation process, and especially can be used for a reaction process.

The method for producing recombinant human epidermal growth factor by using genetically-engineered colibacillus includes the following steps: engineering bacterium fermentation: activating stored colibacillus by means of culture medium, fermenting main medium and introducing the human epidermal growth factor into fermentation cutlure liquor; expanding bed adsorption; centrifugalizing fermentationculture liquor, adding supernatant fluid into the expanding bed to make adsorption and collecting elution peak containing human epidermal growth factor; gel separation; adding collected eluent into gel chromatographic column, separating and collecting human epidermal growth factor elution peak; freezing, sublimating and drying; freezing collected liquor at -40 deg.C, sublimating and drying so as to obtain the invented product.

The invention discloses an expanded bed adsorption medium for separating antibodies by taking mercapto ehtylpyridine and sulfonyl groups as petunidins, and a preparation method. In the composition of the medium, interstitial substance is cellulose / inorganic weighting-agent composite microspheres, and the petunidins are sulfonyl groups or mercapto ehtylpyridine. The cellulose / inorganic weighting-agent composite microspheres which are used as the interstitial substance are obtained by adopting an opposite-phase suspension thermal-reproduction method; dried activation interstitial substance, themercapto ehtylpyridine and sodium hydroxide containing ammonium persulfate are mixed for coupling to obtain the expanded bed adsorption medium for separating the antibodies by taking the mercapto ehtylpyridine and the sulfonyl groups as the petunidins. The novel expanded bed adsorption medium developed in the invention enhances the selectivity of mercapto ehtylpyridine petunidin to the antibodiesthrough bring in the sulfonyl groups, has good adsorption separation selectivity to the antibodies, and can be used for the large-scale preparation of the antibodies.

The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspension from step (iii) to an expanded bed adsorption process obtaining the purified protein product.

The invention relates to a method of producing gentamicin B with gentamicin B fermentation liquid. The method includes the steps of: 1) regulating pH value of the gentamicin B fermentation liquid to 3-5 with phosphoric acid, fully stirring the fermentation liquid, adding CaO to regulate the pH of the fermentation liquid to 5-6, fully stirring the fermentation liquid, successively adding a heavy metal precipitant and a co-filtering agent to treat the fermentation liquid, and performing plate-and-frame filtration and chloroform layering to obtain a gentamicin B water solution; 2) performing ceramic membrane filtration, expanded bed adsorption, crystallization and drying to produce a gentamicin B pure product. The method achieves stable and high-effective production of the gentamicin B, increases extraction yield and effective content of the gentamicin B, is simple in processes and low in production cost, reduces environmental pollution index and reduces environmental protection treatmentcost.

A separation column for expanded bed adsorption comprises a column tube (2), a base (15) carrying an inlet rotor structure (6) for pumping in process liquid, and a top cap (3). The top cap (3) is conical in form, and has a peripheral flange 31 by which it is rigidly fixed to the top edge flange (22) of the column tube (2). The angle of the convergent interior surface (35) of the conical top cap (3) may be between 10 and 25°. A vortex-inhibitor component (8) projects down below the outlet structure (4) at the top of the cap, projecting into the operating space (15) of the column to inhibit rotation of liquid in the column interior. An expanded bed adsorption process is done with upward flow of liquid in the column through a bed of media particles.

The invention discloses a simple and fast method for improving the separation efficiency of a blood coagulation factor VIII from its analogs, and the purity and biological specific activity of the blood coagulation factor VIII. The method comprises the following steps: processing hydrophilic gel microspheres in an activator as a matrix to prepare an activated matrix, and bonding the activated matrix with a blood coagulation factor VIII specificity affinity small peptide ligand through a coupling process to realize the covalent connection between the matrix and the blood coagulation factor VIII specificity affinity small peptide ligand. The separation medium is used for the industrialized large-scale production of the blood coagulation factor VIII through cooperating with affinity chromatography chromatogram or expanded bed adsorption, is highly cost-competitive, allows highly pure blood coagulation factor VIII products to be directly extracted from biological raw materials comprising blood, serum, blood plasma, blood plasma fractions, cell culturing solutions, cell homogenates, gene engineering cell culturing solutions and the like only through one step, and realizes time saving and convenience.

The invention provides a polyamine-modified chitosan base expanded bed adsorbing medium and a preparation method of the polyamine-modified chitosan base expanded bed adsorbing medium, and relates to an adsorbing medium and a preparation method of the adsorbing medium. The matrix is a chitosan / stainless steel powder composite micro-ball, and the genin is induced phenyl by activating benzaldehyde and the re-coupled polyethylene polyamine by activating epichlorohydrin. The preparation method comprises the following steps of: adopting a reversed phase suspension crosslinking method to prepare the chitosan / stainless steel powder composite micro-ball which is taken as a blank matrix; and adopting a method that two genins are designed in a dividing way to firstly react chitosan unit molecule C2 site amino in the micro-ball matrix with benzaldehyde, build the hydrophobic interacted genin, activate matrix chitosan unit molecule C6 site hydroxyl by epichlorohydrin, and couple polyethylene polyamine, so as to obtain the expanded bed adsorbing medium which takes the phenyl and the polyethylene polyamine as the genins. The expanded bed adsorbing medium provided by the invention has the ion exchange genin and the hydrophobic genin, and the absorbing and separating efficiency and the selectivity of target matter in feed liquid can be improved, so that the biological substance such as the protein and the like can be separated and purified on a large scale.

The invention discloses a method for adsorbing and separating human serum albumin by expanded bed adsorption based on a mixed mode, which can directly separate and recombine human serum albumin from a yeast fermentation solution, and belongs to the protein separation technology in the biochemical field. The method comprises the following steps: (1) preprocessing a fermentation solution; obtaining the yeast fermentation solution containing the human serum albumin, adding sodium caprylate, and heating and deactivating protease; (2) performing expanded bed adsorption, directly separating the yeast fermentation solution by using an expanded bed filled with a mixed-mode adsorption agent, and collecting elution peaks; and (3) desalting and drying: desalting a collected solution, freeze drying to obtain the human serum albumin with the purity greater than 95 percent. The invention is characterized by developing a novel separation process, so that the high-purity recombined human serum albumin can be directly separated from the yeast fermentation solution. A critical point of the invention lies in adopting the expanded bed adsorption agent based on the mixed mode, cells do not need to be removed from the yeast fermentation solution, the ion strength does not need to be adjusted, the elution condition is moderate, and the method has the characteristics of simple process, high separation efficiency and high yield.