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Preparation and application of composite TiO*copolymer microspheres

A technology of copolymers and microspheres, applied in other chemical processes, organic chemistry, chemical instruments and methods, etc., to achieve good biocompatibility, good hydrophilicity, and simple preparation process

Inactive Publication Date: 2009-09-16
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic copolymers have been widely used as matrices in fixed bed chromatography, but rarely reported as matrices for expanded bed adsorbents.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Add 4g of gelatin, 21g of sodium chloride and 500g of deionized water into a 1L three-necked flask equipped with a stirrer, condenser and thermometer, and heat up to 60°C and stir for half an hour;

[0026] 2) 30g of surface-modified TiO 2 Add to the mixed solution of 100g glycidyl methacrylate, 64g divinylbenzene, 1.4g benzoyl peroxide, 44.2g polyethylene glycol 400 and 18g butyl acetate, stir to obtain a stable dispersed organic phase solution;

[0027] 3) Slowly add the organic phase solution obtained in step 2) into the aqueous phase obtained in step 1), adjust the stirring speed to 350rpm, keep the speed constant, suspend and disperse at 60°C for 45min, raise the temperature to 90°C, keep it warm for 9h, and solidify The white microspheres were obtained, filtered, washed with hot water 5 times, extracted with acetone to remove the porogen, and vacuum-dried to a constant weight to obtain copolymer microspheres;

[0028] 4) Weigh 100 g of the copolymer microspher...

Embodiment 2

[0033] 1) Add 4g of gelatin, 21g of sodium chloride and 500g of deionized water into a 1L three-necked flask equipped with a stirrer, condenser and thermometer, and heat up to 60°C and stir for half an hour;

[0034] 2) 50g of surface-modified TiO 2 Add the mixed solution of 100g glycidyl methacrylate, 55g divinylbenzene, 1.4g benzoyl peroxide, 90.2g polyethylene glycol 400 and 36.5g butyl acetate, and stir to obtain a stable dispersed organic phase solution;

[0035] 3) Slowly add the organic phase solution obtained in step 2) into the aqueous phase obtained in step 1), adjust the stirring speed to 480rpm, keep the speed constant, suspend and disperse at 60°C for 45 minutes, raise the temperature to 90°C, keep it warm for 9h, and solidify The white microspheres were obtained, filtered, washed with hot water 5 times, extracted with acetone to remove the porogen, and vacuum-dried to a constant weight to obtain copolymer microspheres;

[0036] 4) Weigh 80g of copolymer microsph...

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Abstract

The invention relates to a method for preparing composite TiO2 copolymer microspheres and application of the composite TiO2 copolymer microspheres in natural product separation and purification. According to the method, an oil phase containing surface-modified TiO2, benzoyl peroxide serving as an initiator, GMA serving as a monomer, DVB serving as a crosslinking agent and the oil phase of a pore forming agent is added into a composite aqueous phase containing gelatin and sodium chloride, and the mixture is heated to cure to form microspheres; and beta-CD is directly immobilized on the microspheres serving as a immobilization matrix in the presence of sodium hydride to form the composite TiO2 copolymer microspheres. The copolymer microspheres can be used as an expanded bed adsorption medium to separate isoflavones from soybean waste molasses and purify the isoflavones. The method has the advantages of simple preparation process and easy control and amplification. The prepared TiO2 copolymer microspheres have the advantages of good medium sphericity, mild elution conditions, high biocompatibility, excellent hydrophilicity and wide application prospect in the natural product separation and purification.

Description

technical field [0001] The present invention specifically relates to a composite TiO 2 Preparation of copolymer microspheres and its application in separation and purification of natural products (soybean isoflavones). Background technique [0002] Expanded Bed Adsorption (EBA) technology is a new protein separation and purification technology developed in the 1990s, which can directly capture target products from fermentation broth, cell culture fluid or homogenate, and collect solid-liquid separation. , Concentration and preliminary purification in one operating unit, which reduces operating steps, increases product yield, reduces purification costs and capital investment, and is known as the first new unit operation that has appeared in recent years. The operating characteristics of the expanded bed are between the fluidized bed and the fixed bed. The adsorbent is suspended in the bed by the countercurrent movement of the liquid. At the same time, the density and particl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/10C08F2/44C08F4/34C08K3/22C08J9/04C07D311/36B01J20/285
Inventor 袁其朋肖正发宋航彬
Owner BEIJING UNIV OF CHEM TECH
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