32results about How to "Mild elution conditions" patented technology

The invention relates to a method for separating immune globulin IgG from human serum. The method comprises the following steps: 1) diluting the human serum by a buffer solution, adjusting a pH to 6.0to 8.0 and filtering by a filter membrane to obtain a human serum sample; 2) feeding the human serum sample into a chromatographic column, washing by a balancing buffer solution, then eluting by a eluting buffer solution and collecting eluent to obtain the immune globulin IgG solution, the chromatographic column is filled with a combined type bionic chromatography medium, the combined type bionicchromatography medium is prepared from a chromatography matrix and combined type ligand, the chromatography matrix is a hydrophilic porous microsphere with hydroxyl, and a sequence of the combined type ligand is phenylalanine-tyrosine-glutamine-5-aminobenzimidazole; 3) performing utlrafiltration and concentration on the immune globulin IgG solution to obtain the immune globulin IgG. The method can improve an efficiency and a purity for separating the immune globulin IgG from blood.

The invention belongs to the protein chromatographic separation technology in the field of biochemical industry and discloses a mixed model chromatographic method for separating human serum albumin from yeast fermentation liquid. The method includes steps: 1) fermentation liquid pretreatment, to be more specific, removing yeast cells by centrifuging the yeast fermentation liquid which contains the human serum albumin, adding sodium caprylate, heating to remove impurity proteins and inactivating protease, performing centrifugal separation, and taking supernatant; 2) column chromatography, to be more specific, adopting a mixed mode medium with tryptophan as ligand, performing fixed bed chromatographic separation of the supernatant, and collecting elution peaks, wherein a sample loading pH value is 4.0-4.5, and an elution pH value is 7.0-9.0; 3) desalting and drying, desalting collected liquid, performing freeze drying to obtain the human serum albumin with purity larger than 95%. The mixed model chromatographic method is characterized in that by development of a novel separation process, the high-purity human serum albumin can be directly separated from the yeast fermentation liquid. By adoption of the mixed mode medium with tryptophan as the ligand, ion strength adjustment of the yeast fermentation liquid is avoided, adsorption under acid conditions and elution under neutral and weak alkali conditions are realized, and the method has advantages of mild elution conditions, simple process, high separation efficiency and high yield.