Method for preparing microspheric PGDT separating medium with two kinds of pore forms

A separation medium and microsphere technology, applied in chemical instruments and methods, ion exchange, ion exchange regeneration, etc., can solve the problems of irregular shape, easy to break, application limitations, etc., achieve weak non-specific adsorption, convenient operation, low cost effect

Inactive Publication Date: 2005-12-28
TIANJIN UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the two types of pore-type separation media prepared by in-situ polymerization are prone to breakage at high flow rates due to their irregular shapes, so their applications are strictly limited.

Method used

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  • Method for preparing microspheric PGDT separating medium with two kinds of pore forms
  • Method for preparing microspheric PGDT separating medium with two kinds of pore forms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Weigh 5.00 grams of glycidyl methacrylate (GMA), 1.70 grams of divinylbenzene (DVB), 1.46 grams of triallyl isocyanurate (TAIC), 1.84 grams of toluene, 1.33 grams of n-heptane, 0.12 Add 12.9 grams of calcium carbonate to a 50ml Erlenmeyer flask and mix evenly. Prepolymerize for 24 hours in a super constant temperature water bath at 40°C. In a three-necked flask equipped with a stirrer, a reflux condenser and a thermometer, after adding 1% polyvinyl alcohol solution, the reaction mixture was added. Under the protection of nitrogen, adjust the stirring speed. After the droplets are dispersed into an appropriate particle size, slowly raise the temperature to 65°C at a speed of 1°C / 3 minutes, react for 3 hours, then raise the temperature to 75°C at the same speed, and react for 1 hour. Finally, the temperature was raised to 85° C., and the reaction was carried out for 2 hours. Then the polymer microspheres were transferred into nylon sandbags and extracted with ethanol fo...

Embodiment 2

[0018] Weigh 5.00 grams of GMA, 1.01 grams of DVB, 1.05 grams of TAIC, 1.19 grams of toluene, 0.87 grams of n-heptane, and 0.12 grams of azobisisobutyronitrile into a 50ml Erlenmeyer flask, mix well, add 14 grams of calcium carbonate, mix uniform. According to the method in Example 1, 6.0 grams of PGDT two-type pore separation medium that can be used for protein adsorption in anion exchange mode was synthesized. The volume average particle size is 49.1μm, and its specific surface area is 28.7m 2 / g, the static adsorption capacity is 34.9mgBSA / g wet separation medium. Column packing by gravity settling method. At a flow rate of 180.0 cm / h, the dynamic adsorption capacity reached 13.4 mgBSA / ml column volume (21.3 mgBSA / g wet separation medium).

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Abstract

The invention discloses a preparation method of a microsphere PGDT separation medium with two types of pores. The method is to mix the monomer, cross-linking agent, liquid porogen, and initiator first, then add the solid porogen and mix evenly, and form microspheres through suspension polymerization, extract the microspheres with absolute ethanol, and pickle , and dry, two types of pore-type separation media can be obtained. It is characterized in that: the solid porogen is calcium carbonate, and the dosage accounts for 10-3% of the volume content of the reaction mixture; the volume ratio of the solid porogen to the liquid porogen is 0.5-1; the dosage of the porogen accounts for 3% of the volume content of the reaction mixture. 20-60%; the amount ratio of the two cross-linking agents is 0.5-2, and the ratio of the amount of cross-linking agent to the amount of monomer is 0.3-0.6; under normal pressure, the polymerization temperature is controlled at 65-85 between ℃. The separation medium prepared by the method can be used for the separation of biomacromolecules such as proteins after modification, has large adsorption capacity and mild elution conditions, and has good application prospects in large-scale preparative separation of biomacromolecules.

Description

technical field [0001] The present invention relates to a method for preparing PGDT with two types of holes by means of suspension polymerization, i.e. poly(glycidyl methacrylate-divinylbenzene-triallyl isocyanurate) microsphere separation medium, It belongs to the chromatography medium preparation technology used in the separation and purification process of biological macromolecules. Background technique [0002] In the separation and purification process of biological macromolecules, liquid chromatography is recognized as a very effective means. With the increasing demand for biological products, the performance requirements of liquid chromatography media are also getting higher and higher. Therefore, the preparation of chromatographic media with excellent performance has become an important topic in the research of bioseparation technology. [0003] In the past forty years, many membranes and macroporous polymer particles with pore sizes of 10...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/08B01J20/26B01J20/30C08G18/00C08L9/10
Inventor 孙彦施扬董晓燕白姝
Owner TIANJIN UNIV
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