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A nitrogen-heterocyclic organic polymer monolithic material and its preparation and application

A monolithic material, nitrogen heterocycle technology, applied in the field of biomedical materials, can solve the problems of technical stability and reusability, no small molecule ligand investigation, increase the complexity of the preparation process, etc., and achieve a simple and fast preparation strategy. , low cost, low price effect

Active Publication Date: 2021-11-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether it is HLAC or HCIC technology, they also have the following defects: 1. They usually use agarose as a matrix, so they may have poor mass transfer and mechanical strength, which will affect the enrichment and purification effect[Journal ofproteome research, 2006,5(5):1186-1194.]; 2. The preparation of chromatographic materials usually undergoes complex steps such as matrix activation and ligand grafting, which not only increases the complexity of the preparation process but also affects the stability and stability of the technology. Reusability brings difficulties [JournalofChromatographyA, 1369(2014) 116–124]; 3. Due to the lack of specificity will bring some non-specific adsorption, so few ligands can be successfully used in complex biological samples Enrichment and purification of monoclonal antibody drugs
But so far there is still no research to investigate the small molecular ligands of individual nitrogen-containing heterocycles

Method used

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  • A nitrogen-heterocyclic organic polymer monolithic material and its preparation and application
  • A nitrogen-heterocyclic organic polymer monolithic material and its preparation and application
  • A nitrogen-heterocyclic organic polymer monolithic material and its preparation and application

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Effect test

Embodiment 1

[0048] The functional monomer 1-vinylimidazole (VIM), cross-linking agent EDMA, porogen (composed of n-propanol, n-decyl alcohol and deionized water) and initiator AIBN were formulated into a polymerization reaction mixed solution according to an optimized ratio, and ultrasonically After dissolving and degassing, it was poured into a 100 μm capillary tube pretreated with γ-MAPs, and reacted in a 60°C water bath for 1.5 hours; the unreacted solution was washed away with methanol to obtain a PVIM organic polymer monolithic chromatographic column. Wherein the mass ratio of monomer VIM and porogen is 22:78, the mass ratio of monomer VIM and crosslinking agent EDMA is 50:50, and the mass ratio of porogen n-propanol, n-decyl alcohol and deionized water is 52.9 :11.8:35.3, the quality of initiator AIBN is 1% of monomer VIM.

[0049] The scanning electron microscope (SEM) and the energy distribution surface scanning analysis diagram (Elemental mapping) of the internal morphology of th...

Embodiment 2

[0051] The functional monomer 1-vinylimidazole (VIM), cross-linking agent EDMA, porogen (composed of n-propanol, n-decyl alcohol and deionized water) and initiator AIBN were formulated into a polymerization reaction mixed solution according to an optimized ratio, and ultrasonically After dissolving and degassing, pour it into a 200 μL sealed pipette tip, and react in a water bath at 60°C for 1.5 hours; after the reaction is completed, unseal the tip of the pipette, connect the tip of the pipette to the syringe, and rinse it off with methanol The unreacted solution was obtained as a PVIM organic polymer monolithic chromatographic column. Wherein the mass ratio of monomer VIM and porogen is 22:78, the mass ratio of monomer VIM and crosslinking agent EDMA is 50:50, and the mass ratio of porogen n-propanol, n-decyl alcohol and deionized water is 52.9 :11.8:35.3, the quality of initiator AIBN is 1% of monomer VIM.

Embodiment 3

[0053] The functional monomer 1-vinylimidazole (VIM), cross-linking agent EDMA, porogen (composed of n-propanol, n-decyl alcohol and deionized water) and initiator AIBN were formulated into a polymerization reaction mixed solution according to an optimized ratio, and ultrasonically After dissolving and degassing, pour it into a 200 μL sealed pipette tip, and react in a water bath at 60°C for 1.5 hours; after the reaction is completed, unseal the tip of the pipette, connect the tip of the pipette to the syringe, and rinse it off with methanol The unreacted solution was obtained as a PVIM organic polymer monolithic chromatographic column. Wherein the mass ratio of monomer VIM and porogen is 27:73, the mass ratio of monomer VIM and crosslinking agent EDMA is 50:50, and the mass ratio of porogen n-propanol, n-decyl alcohol and deionized water is 52.9 :11.8:35.3, the quality of initiator AIBN is 1% of monomer VIM.

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Abstract

The invention belongs to the field of biomedical materials, and discloses a nitrogen-heterocyclic organic polymer integral material as well as its preparation and application. The preparation method is as follows: mixing nitrogen-heterocyclic monomer compounds, cross-linking agents, porogens and initiators, then ultrasonically dissolving, degassing, and pouring them into a container; polymerization reaction to obtain the nitrogen-heterocyclic organic polymer integral material. The nitrogen-heterocyclic organic polymer integral material obtained in the present invention can be used for capturing monoclonal antibody drugs in complex biological samples. It has the advantages of simple and rapid preparation strategy, low cost, good permeability, stable physical and chemical properties, long service life, less non-specific adsorption, mild elution conditions and will not damage the conformation and activity of antibody proteins.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a nitrogen-heterocyclic organic polymer integral material and its preparation and application. Background technique [0002] Due to its excellent efficacy, monoclonal antibody drugs have become one of the fastest-growing biological drugs for the treatment of various cancers, infectious diseases, and autoimmune diseases [Nature Reviews Immunology,2010,10(5):317.] . According to statistics, the global monoclonal antibody market reached US$135 billion in 2018, and is expected to reach US$212 billion by 2022. Therefore, the market value is huge and the economic benefits are very considerable [MonoclonalAntibodies (MAbS) Global Market Report 2020]. The sharply increasing demand for monoclonal antibody drugs in the biopharmaceutical market has driven the increase of drug titers in upstream production, which has also added a heavy burden to the current downstream purific...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F226/06C08F222/14C08J9/28C08L39/04B01J20/26B01J20/30C07K16/06C07K16/00C07K1/14
CPCB01J20/267C07K1/14C07K16/00C07K16/065C08F226/06C08J9/28C08J2339/04
Inventor 王启钦江正瑾雷雨田刘潇徐蓉蓉沈媛
Owner JINAN UNIVERSITY
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