288 results about "Specific adsorption" patented technology

The invention provides a nylon 6 nanofiber virus-removing filtering membrane and a preparation method and application thereof.The nylon 6 nanofiber virus-removing filtering membrane comprises a base material and a nylon 6 nanofiber membrane compounded to the surface of the base material, the average pore size of the virus-removing filtering membrane is 20 nm, the virus-removing filtering membrane plays a role in blocking parvoviruses, the surface of the virus-removing filtering membrane is charged, and the surface of the virus-removing filtering membrane is not prone to being damaged. Proteins with the same charges on the surface can be prevented from adhering to the surface of the fiber. In the preparation process of the virus-removing filtering membrane, the nylon 6 nanofibers with a certain diameter range are selected and compounded on the surface of the base material in a stable dispersion state, the uniform microporous virus-removing filtering membrane is prepared, the effect that parvoviruses are blocked and protein molecules are not easily intercepted is achieved, the hydrophilicity of the nylon 6 nanofibers is relatively good, and the virus-removing filtering membrane has a good application prospect. The non-specific adsorption of the filtering membrane on the protein is reduced; the nylon 6 nanofiber virus-removing filtering membrane can be applied to the field of biological medicine products, and has important significance on separation and purification of biological preparations.

The invention discloses a novel herpes zoster vaccine composition and a preparation method, and belongs to the technical field of vaccines. The novel herpes zoster vaccine composition comprises gE protein and an adjuvant, the adjuvant is selected from at least one of an aluminum salt adjuvant, a saponin adjuvant, a TLR pathway agonist, an STING pathway agonist, an emulsion adjuvant and a liposome adjuvant. The invention also provides a preparation method of the composition. Compared with the prior art, the gE protein prepared by the method disclosed by the invention has the advantages that a protective matrix is jointly constructed by saccharides capable of forming a glassy state and a buffer system, and conformation is fixed through hydrogen bond replacement and vitrification in freezing and drying processes, so that interface-induced folding and aggregation are reduced; a small amount of surfactant weakens air-liquid and solid-liquid interfacial tension, terminal low-adsorption filtration reduces non-specific adsorption loss, and the monomer state and immunogenicity are maintained after redissolution.

The invention provides an electrochemical biosensor based on ultrasonic bidirectional driving and a detection method, and belongs to the technical field of biosensing. The sensor comprises a porous membrane and an ultrasonic bidirectional driving unit, and a probe capable of specifically capturing a target object is fixed in a pore channel of the porous membrane; the ultrasonic bidirectional driving unit is composed of a first ultrasonic transducer and a second ultrasonic transducer which are symmetrically arranged on the two sides of the porous membrane, and can synchronously execute reverse power gradient change to form alternately changing pressure difference on the two sides of the membrane so as to drive liquid to repeatedly penetrate through the porous membrane. The detection method comprises the steps of to-be-detected liquid injection and ultrasonic bidirectional driving, ultrasonic auxiliary cleaning and electrochemical detection. The problems of insufficient probe utilization rate, low mass transfer efficiency and non-specific adsorption of a traditional passive diffusion mechanism are solved by utilizing bidirectional ultrasonic active driving force, the detection sensitivity, speed and signal-to-noise ratio are remarkably improved, and the method is suitable for rapid and high-sensitivity quantitative analysis of various analytes.

The invention provides a high-concentration volatile organic compound shallow cooling adsorption and desorption method based on filling type adsorption, and belongs to the technical field of waste gas treatment. According to the method, the pore structure parameters of a model adsorption material with concentrated pore size distribution and the static adsorption isotherm and dynamic penetration curve of VOCs at different temperatures are tested; a linear relation equation of critical aperture and critical pressure is established through fitting, a correlation equation of the critical aperture, temperature and partial pressure is constructed, dynamic adsorption capacity is calculated, and the low-temperature strengthening method suitable for dynamic adsorption is determined by combining a shallow cold adsorption and desorption strategy. According to the method, a ternary matching relation equation among VOCs partial pressure, adsorbent aperture and adsorption temperature is constructed, the critical aperture under a specific adsorption condition is calculated, a low-temperature enhanced VOCs shallow cold adsorption and desorption technology is developed, the desorption difficulty is reduced while the pore volume utilization rate and the adsorption capacity are improved, and a support is provided for adsorbent type selection and adsorption technology optimization.

The invention provides a pepsinogen II determination kit and a quantitative determination method, the kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a luminous donor molecule labeled pepsinogen II first antibody and a reaction buffer solution; and the R2 reagent comprises a light-emitting receptor molecule labeled pepsinogen II second antibody and a reaction buffer solution. A double-antibody sandwich structure marked by a luminescent donor and a luminescent receptor respectively is adopted, binding and luminescence can be completed in a liquid phase, solid phase coating and subsequent multi-step washing are not needed, real homogeneous detection and one-step incubation are achieved, the operation process is simplified, the detection time is shortened, and a full-automatic platform is adapted; and an improved buffer system can inhibit non-specific adsorption and reduce heterotropism interference such as RF / HAMA, so that the detection accuracy and repeatability are improved.

The invention provides a synthesis method of adsorption resin for removing immune globulin, and relates to a high polymer material synthesis technology, polyethylene glycol (PEG) or sulfobetaine (SBMA) is grafted through RAFT polymerization, the thickness of a grafting layer is 50-150nm, the nonspecific adsorption capacity of albumin is less than 5ng / cm < 2 >, photosensitive groups (such as phenyl azide) and ultraviolet light (254nm) are introduced to trigger crosslinking, and the coating shedding rate is less than 0.5% / time. According to the present invention, the cyclic stability is improved by 2 times, the Protein A directional capture antibody is grafted on the surface of the acrylic resin grafted with the polyethylene glycol or sulfobetaine, the IgG adsorption capacity achieves 150 mg / g, the specificity is more than 95%, the polyethylene glycol (PEG) is grafted on the surface of the magnetic particle by using ATRP / RAFT controllable polymerization, the organic solvent use amount is reduced by more than 80%, and the product adsorption capacity achieves 150 mg / g.

The invention relates to the technical field of bioengineering, in particular to an exosome separation material with CLIKKPF as a ligand and a preparation method of the exosome separation material. A novel exosome affinity medium is developed by utilizing the Ca < 2 + > dependency recognition characteristic between CLIKKPF and PS, specific adsorption of the exosome is realized by virtue of a water-soluble polymer and a brush-like structure thereof in combination with polypeptide CLIKKPF, and meanwhile, rapid separation of the target exosome is realized by utilizing nano magnetic beads as supporting particles.

Disclosed in the present invention is a monolithic column material. Quaternary ammonium groups are grafted onto the inner surfaces of pores and channels of the monolithic column material. The quaternary ammonium group is present in the form of EP-OCH2CH(OH)CH-N+R1R2R3. The monolithic column prepared in the present invention is rich in hydroxyl, has excellent compatibility with an eluted analyte containing biomacromolecules, has excellent specific adsorption capacity for nucleic acid substances, and is suitable for enrichment and separation of DNA and / or RNA substances.

The invention relates to the technical field of soil remediation, and discloses a soil remediation material for targeted adsorption of perfluoroalkyl acid organic pollutants and a preparation method of the soil remediation material. The soil remediation material is prepared from the following raw materials: microorganisms, a porous carrier, cationic gel, anionic gel and ferrite, wherein the microorganisms have the capability of driving a nitric acid reduction-ferrous oxidation process. According to the soil remediation material provided by the invention, the microorganisms with the capability of driving the nitric acid reduction-ferrous oxidation process, the porous carrier, the cationic gel, the anionic gel and the ferrite are used as raw materials, and the mechanical strength change of the material in different application stages is dynamically regulated and controlled through the valence change of iron ions and the mineralization process of the microorganisms; according to the soil remediation material disclosed by the invention, the miscibility of the soil remediation material and soil and the specific adsorption of the medium-short-chain perfluoroalkyl acid are enhanced, and meanwhile, the conversion of pollutants from an extractable state to a non-extractable state can be promoted, so that the deep fixation of the pollutants is realized, and the secondary release risk after fixation is reduced.

The invention discloses preparation of phenolic chelating resin and application of the phenolic chelating resin in deep defluorination, belongs to the field of water treatment adsorption materials, and particularly relates to a resin adsorbent with a phenolic polycondensation framework, an amino phosphonic acid chelating functional group and a metal coordination center. The structural unit of the resin adsorbent is shown in the figure. The phenolic chelate resin is suitable for a deep fluorine removal process with complex wastewater components, and specific adsorption of F <-> is achieved by loading metal cations (M) such as aluminum, iron, zirconium, lanthanum and cerium. The phenolic chelate resin provided by the invention has a highly hydrophilic skeleton and a uniform mesoporous structure (2-50nm), has good dynamic performance, low affinity to organic matters, strong anti-pollution ability, high adsorption capacity and F-selectivity, and can effectively improve the process operation efficiency and reduce the resin regeneration cost.

A method for measuring the concentration of an analyte on the basis of a periodically variable external regulation force, and an oscillator system, which aim to solve the problem of non-specific adsorption in existing immunoassay techniques leading to reduced sensitivity and specificity in the measurement of analyte concentrations in samples. The measurement method comprises: obtaining a detection system, and setting a periodically variable external regulation force; binding both a capture probe and a detection probe with an analyte in a sample; making labeled particles, which are bound to the detection probe, perform periodic reciprocating motion under the action of the external regulation force; and by means of a monitoring and imaging apparatus, recording motion imaging signals of the labeled particles to select specifically bound molecules, and compiling statistics to calculate the proportion of the specifically bound molecules so as to obtain the analyte concentration. A specifically bound analyte can be accurately selected, thereby enhancing the capability and detection sensitivity of specific detection of analytes. The method has no complex elution processes, has a short detection response time, and exhibits high-sensitivity detection capability in complex environments.

The invention belongs to the technical field of biomedical detection, and particularly discloses a nucleic acid aptamer for specifically recognizing serum alpha fetoprotein and application of the nucleic acid aptamer. The nucleotide sequence of the nucleic acid aptamer comprises a sequence as shown in SEQ ID No.1, or has more than 90% of homology with the sequence as shown in SEQ ID No.1, or one or more nucleotides are deleted or added in the sequence as shown in SEQ ID No.1. The nucleic acid aptamer can realize specific efficient recognition of AFP protein, also shows extremely high selectivity in a complex serum sample environment, eliminates non-specific adsorption interference of homologous protein and other serum interference protein, greatly reduces the false positive rate, and improves the accuracy and specificity of clinical detection. And the material constructed by directionally functionalizing the aptamer on the surface of the magnetic nano-carrier has rapid magnetic response performance, separation and enrichment of target protein can be realized within seconds, and the clinical diagnosis time is greatly shortened.

The invention relates to the technical field of adsorption materials, in particular to modified adsorption resin for a high-impurity n-heptane raw material and a purification method of the modified adsorption resin. The resin is prepared from the following raw materials: polystyrene-divinylbenzene resin microspheres, chloromethyl ether, 1, 3, 5, 7-tetra (4-chloromethylphenyl) adamantane, 2-methylimidazole and the like. During preparation, polystyrene-divinylbenzene resin microspheres are subjected to chloromethylation to introduce active sites, adamantine crosslinking to construct rigid pore channels, and imidazolyl functionalization to introduce specific adsorption sites. During purification, dynamic adsorption is performed after pretreatment of two stages of adsorption columns, mild regeneration is performed by using an ethanol-acetone mixed solution, isoparaffin, aromatic hydrocarbon and oxygen-containing compounds can be synchronously removed, high-purity n-heptane is obtained, the resin regeneration performance is excellent, and the whole process is easy to industrialize.

This invention belongs to the field of biosensing and detection technology, specifically disclosing a Tau protein electrochemiluminescent aptamer sensor, its preparation method, and its application. The sensor uses a CsPbBr₃@PVP / Au composite material as the electrochemiluminescent substrate. A Tau protein-specific aptamer is immobilized on the substrate surface via Au-S bonds, and non-specific adsorption sites are blocked with 6-mercapto-1-hexanol. The CsPbBr₃@PVP / Au composite material is prepared by encapsulating CsPbBr₃ with PVP and then combining it with Au NPs. This invention utilizes the coordination passivation effect of PVP and CsPbBr₃ to significantly improve the aqueous stability and electrochemiluminescence efficiency of perovskite nanocrystals. Combined with the anchoring effect of AuNPs and the specific recognition effect of aptamers, it achieves ultrasensitive detection of Tau protein with a detection limit as low as 0.80 fg / mL. Furthermore, it exhibits excellent selectivity, stability, and detection accuracy in complex biological samples such as cerebrospinal fluid, providing a novel and efficient detection platform for the early diagnosis of Alzheimer's disease and showing promising clinical application prospects.

Disclosed is a monolithic column material, primary amine groups or secondary amine groups or tertiary amine groups being grafted on the internal surfaces of pores and channels of the monolithic column material. The primary amine groups, secondary amine groups or tertiary amine groups exist in the form of EP-OCH2CH(OH)CH-NR1R2. A monolithic column prepared by the present invention is rich in hydroxyl groups, has excellent compatibility with elution objects containing biological macromolecules, and also has excellent specific adsorption capacity for nucleic acid substances, making it suitable for enrichment and separation of DNA and / or RNA substances.

Provided are a test reagent that reduces non-specific adsorption and is capable of detecting a low-concentration target substance, and a detection method including using the test reagent. The test reagent is a test reagent for in vitro diagnosis, and comprises: a thiourea compound; and resin particles, wherein the resin particles each have a sulfur element.

The application discloses a kind of gallium indium selective separation adsorbents and preparation method thereof, belong to functional metallurgical polymer adsorption material technical field.Gallium, indium physical and chemical properties are extremely similar, conventional separation process is extremely difficult, current industrial separation completely relies on organic extraction process, there is long process, organic reagent cost is high, organic waste water pollution is serious, separation precision is low defect;Conventional commercial adsorption resin is poor to gallium, indium selectivity, separation coefficient is extremely low, cannot realize industrialization high-efficiency separation.The application is with macroporous polystyrene resin as matrix, by nitration, reduction, amination, chelation functional group grafting multi-step modification process, and high selectivity gallium indium separation special adsorbent is prepared.The adsorbent can preferentially specific adsorption of gallium ions in acidic system, and indium ions remain in solution, and the separation coefficient of gallium and indium is greater than or equal to 20, which can completely replace traditional extraction separation process, realize extraction-free, green, high-precision, low-cost gallium and indium high-efficiency separation, fill the industry extraction-free gallium and indium industrialization separation material technical blank.

The invention discloses a passive sampling device and method for specifically enriching quinolones and tetracyclines antibiotics, and relates to water body detection. Firstly, a hydrogel thin film prepared from ZIF-C as an adsorbent is adopted as a binding film, ZIF-C particles are uniformly dispersed on the surface of the binding film, specific adsorption of quinolones and tetracyclines antibiotics can be achieved, then the binding film, a diffusion film and a polyether sulfone filtering film are sequentially stacked to be assembled into an o-DGT device, o-DGT is put into a water body, and through diffusion and adsorption, the specific adsorption of the quinolones and tetracyclines antibiotics can be achieved. The method realizes the extraction and detection of quinolone and tetracycline antibiotics by the DGT technology.

The invention relates to the technical field of traditional Chinese medicinal material detection, and particularly discloses an alpha-asarone extracting solution detection method and system based on a biosensor, and the method sequentially comprises the following steps: mixing medicinal material powder to be detected with an extraction solvent according to a certain solid-to-liquid ratio, and extracting volatile oil by adopting an ultrasonic or reflux method to obtain a crude extracting solution; volatile oil concentrated mother liquor is obtained; obtaining a standard solution; a combined-state electrode is obtained; obtaining electrochemical response data; and converting the electrochemical response data into the content of alpha-asarone in the sample according to the standard curve, and evaluating the precision, the recovery rate and the regeneration performance to obtain a final detection result. Recognition sites prepared by adopting the molecularly imprinted polymer are complementarily paired with template molecules in steric configuration, steric hindrance and key functional groups to realize specific recognition of alpha-asarone, so that false signals caused by matrix interference and non-specific adsorption are remarkably reduced; the electrochemical measurement method is extremely sensitive to electron transfer generated by surface bonding.

The invention discloses construction and application of a biosensor based on an efficient antifouling interface and dual signal amplification, and belongs to the technical fields of optical / electric analytical chemistry, biosensing and material science. According to the invention, the electrode interface is modified with polyaniline doped with polytannic acid as an antifouling film, so that non-specific adsorption of interfering biomolecules in a complex medium is resisted; euTb-MOF is prepared as an efficient luminous body, Tb < 3 + > can transfer energy of Tb < 3 + > and ligand to Eu < 3 + >, and effective signal self-enhancement is realized; according to the EuTb-MOF, the reduction of S2O8 < 2-> is promoted by utilizing the reversible conversion of valence states of Co < 2 + > / Co < 3 + > and Fe < 2 + > / Fe < 3 + > and the synergistic catalysis of Ag NPs, so that the electrochemical luminescence signal of the EuTb-MOF is further improved. Based on the above strategy, the constructed biosensor has high detection sensitivity and precision and long service life, the linear range is 10 fM to 100 nM, and the detection limit is as low as 3.62 fM.

The invention relates to a method for removing halogenated alkyl toxic impurities in probenecid sodium, which is characterized by comprising the following steps of: 1, heating and hydrolyzing excessive halogenated alkyl under an alkaline condition to remove the halogenated alkyl toxic impurities; 2, organic solvent extraction and removal; 3, adsorption removal by a specific adsorption column; after the treatment by the method, the residue of haloalkane is reduced to be not detected from 15-100ppm, and the limit requirements of related genotoxic impurities in Chinese drug registration regulations and ICH guidance principles are completely met.

The application discloses an acridinium ester labeled complex, which comprises a body, and a labeling group labeled on the body, wherein the body is an antibody, and the labeling group is an acridinium ester group. A preparation method of the acridinium ester labeled complex comprises the following steps: sucking a labeling buffer into a centrifugal tube; sucking an antibody to be labeled into the centrifugal tube and mixing to generate a first mixed solution; adding an acridinium ester solution and mixing, and then performing reaction at 25 DEG C in the dark for 2 hours to generate a second reaction solution; adding a termination buffer and mixing, and then performing reaction at 25 DEG C in the dark for 30 minutes to generate a third reaction solution; performing desalting purification on the third reaction solution to generate a fourth mixed solution; and filtering the fourth mixed solution with a 0.22 mu m filter membrane to generate the acridinium ester labeled complex. According to the preparation method, the labeling efficiency of the acridinium ester and the antibody is higher, the reactivity is stronger, the non-specific adsorption is lower, the sensitivity is higher, the repeatability and stability are better, and the labeling method is simple, rapid and easy to repeat.

The invention relates to the technical field of N sugar chain detection, and particularly discloses an adsorption material for purifying an N sugar chain, a hydrophilic magnetic sphere containing the adsorption material and a kit. The adsorption material for purifying the N sugar chain, provided by the invention, comprises synthesized 1-fluorobetaine acyl chloride hydrochloride which is a hydrophilic molecule and can be connected to a magnetic microsphere through a bonding effect, so that a new hydrophilic magnetic sphere is obtained. The hydrophilic magnetic sphere combined with the hydrophilic molecule can increase the N sugar chain grabbing efficiency of the magnetic sphere and reduce non-specific adsorption to other molecules such as free dye. When being used for N-sugar spectrum detection of an antibody sample, the N-sugar spectrum detection kit is very good in repeatability, precision, linearity and accuracy indexes.

The application relates to the technical field of analysis and detection, in particular to a detection method for a strong-toxic new pollutant 6PPD-quinone, which comprises the following steps: adopting a magnetic molecular imprinting polymer to perform specific adsorption on 6PPD-quinone in a water sample to be detected, eluting, and obtaining a sample liquid rich in 6PPD-quinone; and then adopting a single-atom colorimetric detection method to detect the sample liquid. The method realizes the enrichment of the target 6PPD-quinone, simplifies the pretreatment steps, releases the hands of the detection personnel, and realizes the high-sensitivity and high-selectivity detection of the content of 6PPD-quinone by combining the single-atom colorimetric detection method, so that the method can break through the limitation of large instruments and provide a brand-new solution for realizing the on-site, rapid and low-cost monitoring of ultra-trace 6PPD-quinone in the environment.

The invention discloses a strong cation exchange chromatography medium as well as a preparation method and application thereof, and belongs to the technical field of biological separation and purification. The structural formula of the strong cation exchange chromatography medium is shown in the specification, the preparation method comprises the following steps: (1) synthesizing epoxy porous polymer microspheres; (2) carrying out an epoxy-sulfydryl click reaction with sulfydryl sulfonate; and (3) hydrolyzing the residual epoxy group to obtain the epoxy resin. The strong cation exchange chromatography medium has the advantages of high protein loading capacity, low non-specific adsorption, good mechanical property and the like, the preparation process is simple, special hydrophilic modification is not needed, and the process is simplified, the industrial production period is shortened and the production cost is reduced under the condition of meeting the conventional protein elution condition. And meanwhile, the high efficiency of the click reaction can meet the characteristics of high loading capacity, good biocompatibility and the like of the chromatography medium.

The invention provides a composite repairing agent capable of synchronously removing heavy metal cadmium and neonicotinoid pesticides and a preparation method and application of the composite repairing agent. The composite repairing agent comprises a functional fungicide and iron minerals, and the ratio of the dry weight of the iron minerals to the volume of a fungicide liquid of the functional fungicide is 1: (1-10); the functional microbial agent is a viable bacterium culture of dissimilatory iron reducing bacteria, the thallus concentration of the functional microbial agent is not lower than 1 * 10 < 8 > CFU / mL, and the iron mineral is Fe2O3.nH2O. According to the method, an iron reducing bacteria-ferrihydrite mixed system formed by in-situ active minerals is constructed, efficient treatment of combined pollution is achieved through an adsorption-conversion-degradation synergistic mechanism, and ferrihydrite is converted into magnetite in situ within 24-72 hours through iron reducing bacteria in the iron reducing bacteria-ferrihydrite; the new magnetite shows excellent dual functions, and the removal rate of Cd can be increased to gt by crystal lattices of the new magnetite through obligate adsorption and coprecipitation; meanwhile, structural ferrous iron is used as a strong reducing agent and can efficiently attack nitryl in imidacloprid molecules, and gt, gt, gt, gt, gt, gt and gt are achieved; the degradation rate is 90%.

The invention is applicable to the technical field of biological detection, and provides a method for detecting staphylococcus aureus based on combination of a screen-printed electrode and CRISPR / Cas13a, a probe containing a DNA / RNA hybrid chain is combined with a magnetic bead to form a simple composite structure, when the Cas13a recognizes 16sRNA of S. aureus, trans-cleavage activity can be immediately activated, the probe chain is rapidly cut off, the cut-off amount depends on the activated amount, and when the Cas13a recognizes 16sRNA of S. aureus, the Cas13a can be used for detecting staphylococcus aureus. And after transient magnetic separation, a result is observed on the screen-printed electrode. The method has ultrahigh specificity: the CRISPR / crRNA sequence ensures that staphylococcus aureus and other microorganisms can be accurately distinguished; the trans-cutting of Cas13a provides chemical amplification, and the magnetic separation purification greatly reduces background signals caused by non-specific adsorption, so that the signal-to-noise ratio and the sensitivity are remarkably improved.

The application discloses an online regeneration type iron ion specific adsorption removal device, which is applied to SCAL type surface condensation type intercooling circulating water treatment of a thermal power plant. The device comprises a bypass pipeline unit, double adsorption treatment units, a bypass backwashing unit, a regeneration liquid unit and the like. The double adsorption treatment units are connected in series between water chambers at the inlet and outlet of a condenser, and the main cycle does not need to be interrupted. The double adsorption treatment units are arranged in parallel and all adopt a three-stage adsorption tank series structure. A monitoring unit monitors iron content of inlet and outlet water, and a control system links all units to realize automatic control. The device removes iron ions efficiently through three-stage gradient adsorption and double-unit alternating online regeneration, synchronously realizes iron product resourceization and wastewater zero discharge, solves the pain points in the prior art, such as poor removal of weak magnetic fine iron, and the fact that adsorption medium cannot be regenerated online, and the like, and is stable in operation and high in automation degree.

The application provides a method for producing lubricating oil base oil through a combined process, which comprises the following steps: carrying out a hydrocracking reaction on raw oil, feeding the obtained hydrocracking tail oil into an adsorption-isomerization reaction zone, and treating the hydrocracking tail oil by contacting the hydrocracking tail oil with a catalyst to obtain a first stream; feeding the first stream into a hydrofinishing reaction zone to carry out a reaction, recycling the reaction effluent of the hydrofinishing reaction zone to the adsorption-isomerization reaction zone for treatment; when the refractive index (20 DEG C) of the first stream is 0.1-3% higher than the refractive index (20 DEG C) of the feed of the adsorption-isomerization reaction zone, stopping feeding the adsorption-isomerization reaction zone, and feeding hydrogen into the adsorption-isomerization reaction zone, so that an isomerization and condensation reduction reaction occurs under the action of the catalyst and the hydrogen; feeding the isomerization and condensation reduction reaction product into a separation system, and separating the isomerization and condensation reduction reaction product to obtain gas, light lubricating oil base oil and heavy lubricating oil base oil.

This invention belongs to the field of environmental microbiology and soil remediation technology, specifically relating to a composite microbial agent, its preparation method, and its application in the remediation of soils contaminated with heavy metals and organic matter. The composite microbial agent provided by this invention combines sulfur-modified biochar, a composite functional microbial community, and slow-release nutrients in a specific ratio. The sulfur-modified biochar contains sulfur-containing functional groups such as S=O and C-S, enabling specific adsorption and fixation of heavy metals. The composite functional microbial community consists of organic pollutant-degrading bacteria, plant growth-promoting bacteria, and heavy metal-converting bacteria in a specific ratio of viable bacteria, complementing each other to synergistically complete the degradation of organic matter and the conversion of heavy metals. The slow-release nutrient component provides long-term nutritional support. The preparation method of this composite microbial agent is simple and suitable for the remediation of heavy metal-organic matter contaminated soils in industrial, mining, and chemical sites, offering advantages such as high efficiency, long-term effectiveness, low cost, and environmental friendliness.