53 results about "Specific adsorption" patented technology

This invention belongs to the field of biosensing and detection technology, specifically disclosing a Tau protein electrochemiluminescent aptamer sensor, its preparation method, and its application. The sensor uses a CsPbBr₃@PVP / Au composite material as the electrochemiluminescent substrate. A Tau protein-specific aptamer is immobilized on the substrate surface via Au-S bonds, and non-specific adsorption sites are blocked with 6-mercapto-1-hexanol. The CsPbBr₃@PVP / Au composite material is prepared by encapsulating CsPbBr₃ with PVP and then combining it with Au NPs. This invention utilizes the coordination passivation effect of PVP and CsPbBr₃ to significantly improve the aqueous stability and electrochemiluminescence efficiency of perovskite nanocrystals. Combined with the anchoring effect of AuNPs and the specific recognition effect of aptamers, it achieves ultrasensitive detection of Tau protein with a detection limit as low as 0.80 fg / mL. Furthermore, it exhibits excellent selectivity, stability, and detection accuracy in complex biological samples such as cerebrospinal fluid, providing a novel and efficient detection platform for the early diagnosis of Alzheimer's disease and showing promising clinical application prospects.

The application discloses an acridinium ester labeled complex, which comprises a body, and a labeling group labeled on the body, wherein the body is an antibody, and the labeling group is an acridinium ester group. A preparation method of the acridinium ester labeled complex comprises the following steps: sucking a labeling buffer into a centrifugal tube; sucking an antibody to be labeled into the centrifugal tube and mixing to generate a first mixed solution; adding an acridinium ester solution and mixing, and then performing reaction at 25 DEG C in the dark for 2 hours to generate a second reaction solution; adding a termination buffer and mixing, and then performing reaction at 25 DEG C in the dark for 30 minutes to generate a third reaction solution; performing desalting purification on the third reaction solution to generate a fourth mixed solution; and filtering the fourth mixed solution with a 0.22 mu m filter membrane to generate the acridinium ester labeled complex. According to the preparation method, the labeling efficiency of the acridinium ester and the antibody is higher, the reactivity is stronger, the non-specific adsorption is lower, the sensitivity is higher, the repeatability and stability are better, and the labeling method is simple, rapid and easy to repeat.

This invention belongs to the field of environmental microbiology and soil remediation technology, specifically relating to a composite microbial agent, its preparation method, and its application in the remediation of soils contaminated with heavy metals and organic matter. The composite microbial agent provided by this invention combines sulfur-modified biochar, a composite functional microbial community, and slow-release nutrients in a specific ratio. The sulfur-modified biochar contains sulfur-containing functional groups such as S=O and C-S, enabling specific adsorption and fixation of heavy metals. The composite functional microbial community consists of organic pollutant-degrading bacteria, plant growth-promoting bacteria, and heavy metal-converting bacteria in a specific ratio of viable bacteria, complementing each other to synergistically complete the degradation of organic matter and the conversion of heavy metals. The slow-release nutrient component provides long-term nutritional support. The preparation method of this composite microbial agent is simple and suitable for the remediation of heavy metal-organic matter contaminated soils in industrial, mining, and chemical sites, offering advantages such as high efficiency, long-term effectiveness, low cost, and environmental friendliness.

This application discloses a chemiluminescence detection kit for acrylonitrile magnetic microparticles and its detection method. The kit includes a magnetic bead working solution and a luminescent label. The magnetic bead working solution includes acrylonitrile monoclonal antibody A and streptavidin. The luminescent label includes acrylonitrile monoclonal antibody B, modified nano-silica, anhydrous toluene, methoxy polyethylene glycol-succinimide ester, N,N-dimethylformamide, acrylonitrile succinimide ester, glutaraldehyde, and ethanolamine. This application uses modified nano-silica as a carrier and grafts a polyethylene glycol hydrophilic layer onto its surface, which amplifies the acrylonitrile ester molecule signal, reduces non-specific adsorption, and improves the sensitivity, specificity, and reliability of the detection.

The present application relates to the field of micro-nano magnetic material for in vitro detection, and particularly relates to a preparation method of high-loading streptavidin magnetic microspheres, the magnetic microspheres and application thereof; in the preparation process, first, streptavidin is reacted with a crosslinking agent to obtain polymerized streptavidin, and then the polymerized streptavidin is coupled on the surface of the magnetic microspheres to obtain streptavidin magnetic microspheres; wherein the structure of the crosslinking agent comprises a hydrophilic flexible chain. The present application has the effects of improving the loading and stability of streptavidin on the surface of the magnetic microspheres, reducing non-specific adsorption, and enhancing the sensitivity of detection.

Disclosed in the present invention are a wastewater adsorbent, and a preparation method therefor and the use thereof. The method comprises: mixing a carbon black powder and an ammonium salt solution, heating same for a hydrothermal reaction, followed by filtering, and washing the obtained filter residues with acid to obtain an ammonium-salt-modified carbon black; mixing and grinding a nickel-cobalt-manganese mixed salt and a sodium salt to obtain a mixture, mixing the mixture with an organic acid solution, evaporating same to remove water, subjecting same to a heating reaction in an inert atmosphere, and subjecting the reacted material to acid pickling to obtain a nickel-cobalt-manganese-sodium mixed salt; and mixing the nickel-cobalt-manganese-sodium mixed salt, the ammonium-salt-modified carbon black and a binding agent, and compacting, drying and heating same to obtain a multimetal-carbon-based adsorbent. The prepared multimetal-carbon-based adsorbent in the present invention has specific adsorption capacities for sodium, ammonium radicals and sulfate radicals; the carbon black powder serving as a substrate carbon material can adsorb multiple ions such as calcium, iron, manganese and cobalt all at the same time, such that diversified adsorption is achieved; in addition, the adsorbent can be reused after a desorption treatment and has a repeat adsorption capacity.

The application provides a Bola type surfactant-based blocking agent, which can be used for modifying various object surface materials, especially the surface of a carrier material for immune diagnosis, so that the non-specific adsorption of the carrier material is reduced, and the sensitivity and signal-to-noise ratio of a reagent are improved. Compared with traditional blocking agents, the Bola type surfactant-based blocking agent provided by the application not only has the excellent characteristics of traditional surfactant blocking agents, but also has increased repulsion to non-specific molecules and more compact blocking due to the rigid hairpin structure after combination.

This invention discloses a magnetic fluorescently imprinted nanoparticle, its preparation method, and its application. The nanoparticle comprises amino-functionalized magnetic nanoparticles and a fluorescently imprinted polymer layer coated on their surface, wherein the fluorescently imprinted polymer layer has an imprinted cavity; the imprinted molecule in the fluorescently imprinted polymer layer is a cTnI-specific C-terminal epitope peptide. Functional monomers are screened using computational chemistry, and nanomolecularly imprinted polymers (MIPs) are synthesized using epitope imprinting technology. Fluorescent monomers are introduced to impart autofluorescence response properties, forming a label-free detection system. These nanoparticles exhibit highly efficient adsorption and specific recognition of cTnI protein, with a detection sensitivity of 1 pg / mL (R²=0.98). They show extremely low non-specific adsorption to common interfering proteins and effectively resist interference from complex biological matrices, providing a new approach for rapid clinical cTnI detection.

This invention discloses a flotation separation method for removing talc from molybdenite using sodium alginate sulfate. The method involves preparing a raw slurry from talc-type molybdenite, controlling the pH of the slurry to be 6.0–10.0. Sodium alginate sulfate is added to the slurry and stirred. Then, an anisopolar collector is added and stirred to condition the slurry. A frother is then added to obtain a mixed slurry. Finally, the mixed slurry is subjected to aerated flotation. The collected frothy product is the molybdenum concentrate, and the product in the tank is the talc-containing tailings. This invention, by adding sodium alginate sulfate (SAS), which has high electronegativity and strong chelating ability, as a specific inhibitor to the flotation system, blocks the contact between the collector and talc. Simultaneously, its strong electrostatic repulsion effectively prevents non-specific adsorption on the negatively charged molybdenite surface. This invention significantly improves the grade and recovery rate of molybdenum concentrate and has extremely high industrial promotion and engineering application value.

The present application relates to the field of separation and purification, and discloses a method for extracting and purifying organic substances from a biological matrix sample, comprising the following steps: after adjusting the pH of the sample to 1-3, performing sample loading and adsorption; through magnetic force, transferring the adsorption material to perform cleaning; after cleaning, before elution, performing infrared or microwave heating on the adsorption material, aiming at evaporating residual solvent, and denaturing and coagulating co-adsorbed proteins to prevent them from being co-eluted, thereby significantly improving the purity of the final eluate; and finally, performing elution on the heated adsorption material. The present application combines special materials and innovative processes, realizes automatic sample pretreatment with high recovery rate and high purity, and can be applied to the extraction of oligonucleotides, peptides, small molecules and the like; and solves the problems of low recovery rate and poor reproducibility caused by non-specific adsorption of proteins in biological samples.

The application discloses a one-step monoclonal antibody drug electrochemical detection biosensor and a preparation method and application thereof. The application designs and screens an affinity specific DNA aptamer hairpin probe and uses the same as a specific capture unit, then uses a gold-sulfur bond to fix the DNA aptamer hairpin probe on a screen-printed electrode surface, meanwhile, uses a self-assembly interaction between a metal and a mercapto group to fix a blocking agent introduced on the screen-printed electrode surface, and blocks the excess electrode surface reaction sites, so that the one-step monoclonal antibody drug electrochemical detection biosensor is obtained. The one-step monoclonal antibody drug electrochemical detection biosensor has the advantages of simple operation, high sensitivity, less non-specific adsorption, high capture efficiency, stable physical and chemical properties, strong specificity and good selectivity, and can be used for capturing antibody drugs in complex biological samples.

This invention discloses a chemiluminescent immunoassay method, comprising the following steps: labeling disulfide-bonded biotin or desulfobiotin onto affinity ligand one to form complex one; labeling a luminescent compound onto affinity ligand two to form complex two; mixing the sample with a solid-phase carrier containing complex one, complex two, and streptavidin-coupled compound to form an immunosandwich complex; processing the immunosandwich complex to release the immunosandwich complex from the solid-phase carrier; separating the immunosandwich complex from the solid-phase carrier; detecting the total luminescent signal value of the separated immunosandwich complex; and calculating the concentration of the analyte based on the total luminescent signal value. This invention releases the immunosandwich complex from the solid-phase carrier, uses a magnetic field to attract the solid-phase carrier, and measures the supernatant of the released immunosandwich complex. Solid-phase carriers containing non-specific adsorption cannot enter the measurement chamber, completely eliminating the problem of non-specific adsorption on the surface of magnetic beads.

The application provides a kind of electrolyte of aqueous zinc ion battery and its preparation method and application, including medium viscosity (200~400 mPa.s) chitosan and L-ascorbic acid, wherein the mass ratio of the chitosan added to the volume of the solvent in the electrolyte is 0.4 g / L~3.2g / L.The electrolyte of the application forms a macromolecular complex through ion adsorption by introducing chitosan and L-ascorbic acid, and establishes a dynamic and dense protective interface on the surface of zinc negative electrode by using its specific adsorption group (such as enediol structure), thereby achieving synergistic effect from two aspects of solvation structure regulation and interface chemical modification, and ultimately improving the cycle life and electrochemical performance of aqueous zinc ion battery.

The present application relates to the field of functional polymer and electrochemistry, in particular to a fluorine-based conductive polymeric gel, its preparation method and application. The present application provides a fluorine-based conductive polymeric gel, which can be used for efficient and green treatment of lithium-containing waste liquid. The gel is prepared by in-situ polymerization of fluorine-containing ester compound and acrylamide as comonomers, combined with crosslinking agent and initiator in organic solvent. By introducing acidified carbon nanotubes and conductive polymer monomers as composite conductive agent, the gel is endowed with excellent conductivity, while retaining the specific adsorption capacity of fluorine-containing functional groups for lithium ions. The present application integrates the fluorine-containing functional groups with specific affinity for lithium ions and the high-conductivity composite conductive network at the molecular level. This integration enables the gel to controllably and reversibly adsorb and desorb lithium ions through electrochemical means, and the regeneration process does not require the use of hydrochloric acid eluent, thereby realizing environmentally friendly and efficient lithium recovery.

This invention discloses a method, product, and application for the separation of astatine-211 in an aqueous phase based on valence state regulation. The method includes: passing a solution of astatine-211 containing a strong oxidizing acid through a sulfur-containing solid support, causing specific adsorption of positively valence astatine-211; eluting with a water-soluble reducing agent to reduce astatine-211 to astatine anions, obtaining an aqueous astatine-211 eluent. The entire process is carried out in the aqueous phase, requiring no organic solvents and avoiding radionuclide volatilization loss due to heating and evaporation. This invention also provides an eluent composition obtained by this method, sodium astatide injection, astatine-211-labeled radiotargeting drugs and their preparation methods, as well as the application of this eluent composition in the preparation of drugs for treating NIS-positive diseases. This invention achieves highly selective separation and mild, rapid elution of astatine-211, and the product exhibits excellent biocompatibility and radiation stability, and can be used directly or after simple activation to prepare targeted α-therapy drugs.

The present application belongs to the field of environmental functional materials and soil improvement technology, and relates to a borate intercalated magnesium-iron bimetallic oxide composite material, a preparation method and application thereof. The material takes straw biochar as a carrier, adopts a coprecipitation method to grow borate intercalated magnesium-iron layered double hydroxide in situ on the surface of biochar, and is prepared by calcination. The material has specific adsorption performance on small-molecule organic carbon sources such as glucose and glutamic acid by using boron hydroxyl functional groups, and still maintains high capacity in a high-salt environment. After being applied to salinized soil, the composite material captures active small-molecule organic carbon by chemical adsorption, significantly improves the microbial biomass carbon content; the proliferation of microorganisms promotes the assimilation and conversion of organic carbon, and finally realizes the increase of total soil organic carbon storage. Through the synergistic mechanism of "chemical capture-microbial driving", the present application realizes the efficient and stable accumulation of organic carbon in salinized soil, and provides a new material and a new way for carbon sequestration and improvement.

This invention discloses a method for preparing an aqueous thermosensitive Cr(III) ion-imprinted polymer, belonging to the field of water pollution treatment technology. The method includes the following steps: (1) dissolving aminated multi-walled carbon nanotubes and potassium carbonate in N,N-dimethylformamide, and adding bromopropylene to obtain vinylated multi-walled carbon nanotubes; (2) dissolving CrCl3·6H2O, a functional monomer, and a thermosensitive monomer in a porous solvent composed of ethanol and water, adding vinylated multi-walled carbon nanotubes, ethylene glycol dimethacrylate, and azobisisobutyronitrile to obtain the Cr(III) ion-imprinted polymer. This invention introduces the thermosensitive functional monomer N-isopropylacrylamide, enabling the ion-imprinted polymer to respond to changes in ambient temperature and accurately identify and adsorb target ions in complex environments. The introduction of the thermosensitive functional monomer allows the polymer to form a hydrophilic layer, reducing non-specific adsorption caused by impurities such as humic acid, and improving the adsorption capacity and selectivity for target ions.

This invention discloses a biomimetic nanofluidic sensing membrane, its preparation method, and its applications. The biomimetic nanofluidic sensing membrane comprises a top supporting lipid bilayer membrane and a bottom anodic aluminum oxide membrane with a macroporous structure. A portion of the supporting lipid bilayer membrane corresponds to and communicates with some macropores in the anodic aluminum oxide membrane. An ion carrier is embedded in the supporting lipid bilayer membrane, and its surface is modified with cholesterol-modified aptamers and polyethylene glycol. This invention utilizes the lateral fluidity of the supporting lipid bilayer to achieve multivalent and efficient capture of aptamers. The natural antifouling properties of phospholipids and the steric hindrance of polyethylene glycol synergistically suppress non-specific adsorption, and the detection signal is amplified by the ion current rectification effect induced by the asymmetric structure. The detection limit for TNF-α is as low as 0.219 pM, and with machine learning, it can accurately distinguish six proteins. The preparation method is simple, and the detection sensitivity and specificity are high, showing broad application prospects in early disease diagnosis and high-throughput protein analysis.

This invention discloses a sulfated modified sodium alginate, its preparation method, and its applications. Urea is heated and melted to obtain urea liquid. Sodium alginate powder is added to the urea liquid to form a dispersion. The mixture is then cooled to 50-70°C, aminosulfonic acid is added, and a protective atmosphere is introduced at a flow rate of 50-200 mL / min. The temperature is then raised to 100-140°C, and the reaction yields sulfated modified sodium alginate. When this product is used as a flotation depressant for magnesium silicate gangues, it utilizes the specific bidentate chelating effect and strong hydration ability of the sulfate ester groups on the talc surface to form a dense molecular shielding film. Simultaneously, it effectively avoids non-specific adsorption on the surface of valuable metal minerals (such as molybdenite) through strong electrostatic repulsion. This invention not only significantly improves the flotation separation efficiency of valuable minerals but also features a simple, environmentally friendly synthesis process with broad industrial application prospects.

This invention discloses a rapid detection method for heavy metals in wastewater based on surface plasmon resonance, belonging to the fields of optical sensing and water quality testing. The invention employs an integrated optical sensing system, consisting of a light source module, a polarization control module, a sensor chip module, a flow control module, a photoelectric detection module, and a signal processing module. The sensor chip module adopts a partitioned heterogeneous functionalized structure, setting up a specific capture region, an inert reference region, and a physical adsorption characterization region. Non-specific adsorption and background interference are eliminated through multivariate differential compensation. The sensing interface constructs a three-layer gradient functionalized structure. This invention achieves trace detection of heavy metal ions in wastewater through a multi-channel differential compensation architecture, functionalized self-assembled monolayers, and a centroid-based signal extraction algorithm. It features strong anti-interference capability, high sensitivity, recyclability, and ease of rapid on-site detection.

This invention provides a method for separating and purifying uronic acid polysaccharides, belonging to the field of polysaccharide separation and purification technology. This invention utilizes the electrostatic adsorption of sodium ions in an aqueous sodium chloride solution around the carboxyl anions of uronic acid units, forming a charge shielding layer. Chloride ions in the aqueous sodium chloride solution are also adsorbed around the dextran gel via electrostatic interaction, effectively shielding the electrostatic attraction between the uronic acid units and the dextran gel, reducing the non-specific adsorption of uronic acid units and the dextran gel, making the uronic acid polysaccharides easy to separate and with high purity. Furthermore, sodium and chloride ions in the aqueous sodium chloride solution are strongly hydrated ions, preferentially binding to the hydroxyl groups on the dextran gel and the carboxyl groups on the uronic acid units, occupying hydrogen bond sites, disrupting the existing hydrogen bonds between the uronic acid units and the dextran gel, preventing the formation of new hydrogen bonds, reducing the adsorption of uronic acid units in the dextran gel, making the uronic acid polysaccharides easy to separate and with high purity.

This invention proposes a method for reducing cadmium in paddy fields using common soil microorganisms, belonging to the field of agricultural technology. Addressing the problems of high cost, secondary pollution, and soil structure damage associated with existing physicochemical cadmium reduction methods, this invention provides a bioremediation technology using Bacillus subtilis ATCC6633 as the functional strain. This method involves activating laboratory-preserved Bacillus subtilis ATCC6633 bacterial powder to prepare a microbial agent, which is then directly applied to the surface water of paddy fields. Utilizing the specific adsorption and conversion of cadmium ions by the strain, effective passivation and removal of cadmium from paddy fields are achieved. This invention uses common soil microorganisms, exhibiting strong environmental compatibility and eliminating the need for chemical agents, fundamentally avoiding the secondary pollution problems caused by traditional chemical precipitation methods. It is both eco-friendly and economically feasible, significantly improving rice quality and providing an effective green remediation approach for cadmium pollution in paddy fields.

The application relates to a silica gel chromatographic column, a filler and a preparation method and application of the filler, the filler is silica gel balls with bonded amino groups and quaternary ammonium groups, and the preparation method comprises the following steps: silica gel ball activation, amino group generation, epoxy group generation and quaternary ammonium group generation steps. The chromatographic column is used for detecting the content of betaine in medlar. Different from the prior art, the filler introduces quaternary ammonium groups with charge shielding effects through bonding of aminopropyl silane to the surface of silica gel, can shield the silica hydroxyl groups to a certain extent, overcomes the non-specific adsorption between the silica hydroxyl groups and betaine, and thus has excellent peak shape, higher column efficiency and stable peak area when used for betaine separation.

The application discloses a separation and purification method for preparing pericarpium citri reticulatae essential oil, and relates to the technical field of pericarpium citri reticulatae essential oil preparation.The system comprises the following steps: S1, filtering pericarpium citri reticulatae crude essential oil through microporous filtration to remove solid suspensions and obtain pretreated essential oil; the application first completes impurity removal, dewaxing and preliminary classification of terpenes through three-stage dynamic molecular distillation, reduces the interference of impurities on subsequent adsorption, then realizes specific adsorption of target components through a D-limonene template molecular imprinting column, and combines supercritical CO2 green desorption to realize accurate separation and directional enrichment of pericarpium citri reticulatae essential oil, effectively solves the problems of non-selectivity of traditional distillation, low adsorption efficiency and difficulty in separating structure-similar terpenes, significantly improves the purity of target components, completely removes wax, pigments and isomer impurities, retains the natural activity and aromatic quality of essential oil, the desorption process has no organic solvent residue, the process has strong adaptability, and can meet the differentiated needs of high-end food, medicine and daily chemicals.

This invention discloses a porous surface-passivated sample cell for optical microscopic observation of liquid-liquid phase separation morphology, its preparation method, and its application. The sample cell includes a glass substrate, multiple independent sample cell units disposed on the surface of the glass substrate, and an adhesive layer for fixing the sample cell units. Each sample cell unit is a ring-shaped structure with through holes, forming multiple independent sample receiving cavities together with the glass substrate. The glass substrate has a surface passivation layer at least in the sample contact area corresponding to the bottom of each sample receiving cavity. The surface passivation layer is a polyethylene glycol-based passivation layer. This invention can significantly reduce the non-specific adsorption of proteins, nucleic acids, and their complex droplets to the bottom surface of the glass, reducing droplet spreading, tearing, and morphological distortion, and more realistically characterizing the intrinsic morphology of liquid-liquid phase separation droplets. It also enables parallel microscopic observation of multiple sample conditions on the same glass substrate, and has the advantages of simple structure, low sample consumption, convenient preparation, and good compatibility with microscopic imaging. This sample cell has completed third-party testing and verification before April 16, 2026.

To provide a method for producing particles that can suppress non-specific adsorption without using BSA. [Solution] A method for producing particles, comprising: a first step of preparing an emulsion by mixing a radically polymerizable monomer, an organosilane compound having silicon atoms to which an alkoxy group is bonded and which is radically polymerizable, a radical polymerization initiator, a water-soluble polymer, and an aqueous medium; and a second step of adding a specific reactive compound after the first step.

The application belongs to the technical field of new materials, and discloses a catalyst applied to electrocatalytic reduction of nitrate to prepare ammonia and a preparation method thereof. The preparation method of the catalyst comprises the following steps: firstly, dissolving copper metal salt, organic acid, phosphomolybdic acid and organic ligand in an organic solvent to prepare a mixed solution; then, performing microwave synthesis on the mixed solution, and preparing a solid powder through separation and drying; finally, performing calcination on the solid powder in an air atmosphere to prepare the catalyst. The application adopts a microwave synthesis coupling air calcination process to prepare a PMo 12 / CuO composite catalyst, realizes in-situ synergistic loading of phosphomolybdic acid (PMo 12 ) and CuO active sites, and the prepared catalyst has Cu-O-Mo and phosphate synergistic active sites, which not only enhances the specific adsorption and activation capacity of the catalyst to nitrate, but also effectively inhibits the hydrogen evolution side reaction, and further improves the selectivity and yield of ammonia.

The application discloses a MOF / MIP / QCM sensor for in-situ detection of quinolone antibiotics, a preparation method and application, and belongs to the technical field of environmental antibiotic detection. The preparation method is as follows: 1) quinolone antibiotic template molecules and functional monomers are added to an organic solvent, and after being dissolved, MOF materials, a crosslinking agent and an initiator are sequentially added to obtain a prepolymerization solution; 2) the prepolymerization solution is subjected to a polymerization reaction under the protection of an inert gas, and after the template molecules are removed by washing, the MOF-based molecularly imprinted polymer is dried to obtain the MOF / MIP / QCM sensor. The sensor is connected with a quartz crystal microbalance detection device, and then placed in a water body to be detected; the specific adsorption of the sensor to quinolone antibiotics causes the characteristic of a decrease in the resonance frequency of the quartz crystal, and the in-situ rapid quantitative detection of quinolone antibiotics is realized by measuring the frequency change.