The invention provides a preparation method and application of a
solid-phase extraction filler and a
solid-phase extraction column for basic dyes in food. Among them, the
solid phase extraction fillers use functional ionic liquids 1-benzyl-3-methylimidazolium
hexafluorophosphate and 1-decyl-3-methylimidazolium
hexafluorophosphate, which are very similar in structure to basic dyes, as the main adsorption The
reagent has strong
specific adsorption capacity for various basic dyes, and does not adsorb impurities such as salts, sugars, pigments, oils, proteins, and starches in food matrices, and can effectively separate impurities and alkalis in the sample to be tested. Sex dyes. In addition,
silica gel is used to immobilize the functional
ionic liquid to form a loose solid-phase extraction filler, which can also be fully contacted with the extraction liquid of the sample to be tested without
extrusion. The sample liquid passes through the packing quickly, which improves the extraction efficiency and shortens the sample pretreatment time. The
solid phase extraction column made of the filler can be used for the rapid determination of the basic dye content in food in
high performance liquid chromatography, and the determination result is accurate and stable.