171 results about "Fluorescence sensing" patented technology

The invention relates to the technical field of fluorescence sensing, in particular to a preparation method and application of an Nd (III)-based hydrogen bond organic framework crystalline material. According to the Nd (III)-based hydrogen bond organic framework crystalline state material, Nd (III) serves as a central metal ion and is coordinated with 9 oxygen atoms, and adjacent Nd (III) are connected through H2 (4-CPCA) ligands to form a one-dimensional chain structure. One-dimensional chains are bridged through H2 (4-CPCA) ligands to form a two-dimensional double-layer planar structure, and two-dimensional surfaces are further stacked through the interaction of a large number of intermolecular hydrogen bonds to form a three-dimensional supramolecular structure. The Nd (III)-based hydrogen bond organic framework crystalline material has good fluorescent property and stability, and can be applied to the field of fluorescence sensing.

The invention discloses a method for distinguishing similar antibiotics by using a fluorescent sensing array based on YOLOv5 target recognition. The method comprises the following steps: S1, preparing GMP-Tb solid powder; s2, mixing the GMP-Tb solid powder with a rhodamine B solution in proportion to obtain a multi-proportion fluorescent probe; s3, mixing antibiotics with different concentrations with the multi-proportion fluorescent probe to obtain a fluorescent sensing array; s4, collecting color images in the fluorescent sensing array and dividing the color images into a training set and a test set; s5, constructing an OLOv5 optic nerve model, inputting the acquired color image into a YOLOv5 optic nerve model, and outputting a feature value of the acquired color image; s6, inputting the characteristic value of the color image into a classifier model for prediction classification to obtain a distinguishing result; and S7, inputting a color image formed by mixing the to-be-detected antibiotic and the multi-proportion fluorescent probe into the YOLOv5 optic nerve model to obtain a characteristic value of the color image, and then outputting a distinguishing result through the classifier model.

The present invention combines a fluorescence sensor with an array to provide a fluorescence sensing array detection platform and a detection method for multiple plasticizers. The fluorescence sensing array detection platform includes a B-doped graphene quantum dot solution using N,N-dimethylformamide as a solvent and a B-GQDs solution using cyclohexane as a solvent; the fluorescence intensity of the B-GQDs solution with DMF as the solvent at the fluorescence characteristic peak obtained under ultraviolet light excitation at a wavelength of 350 nm, and the fluorescence data at 391 nm, 413 nm, and 434 nm are used as array points 1, 2, and 3; the fluorescence intensity of the B-GQDs solution with CH as the solvent at the fluorescence characteristic peak obtained under ultraviolet light excitation at a wavelength of 350 nm, and the fluorescence data at 386 nm, 408 nm, and 430 nm are used as array points 4, 5, and 6; thus, a 3×2 fluorescence sensor array is constructed. The detection method using the fluorescence sensor array of the present invention has simple steps, and has satisfactory specificity, reproducibility, stability and practicability, and has application potential.

The invention provides a ratiometric fluorescence sensing system and a preparation method and application thereof, and the preparation method comprises the following steps: respectively preparing a silicon oxide-based aggregation-induced emission material and a copper-doped carbon dot material, and mixing the silicon oxide-based aggregation-induced emission material and the copper-doped carbon dot material with an ascorbic acid-2-phosphoric acid material to construct the ratiometric fluorescence sensing system. The fluorescent probe is applied to ferric ion detection, in-vitro detection of biomarkers or fluorescence imaging. The technical scheme provided by the invention has good stability, accuracy and practical application potential.

The invention relates to the technical field of fluorescence sensing, in particular to a praseodymium (III) metal organic framework crystalline material and a preparation method and application thereof. According to the praseodymium (III) metal organic framework crystalline state material, Pr (III) is used as a central metal ion and forms eight coordination with eight oxygen atoms of six (4-CPCA) 2-ligands. And adjacent Pr (III) are connected through 4-CPCA < 2-> bridging to form a one-dimensional annular chain. And the one-dimensional chains are further bridged and topologically connected through a (4-CPCA) 2-ligand to form a three-dimensional structure. The praseodymium (III) metal organic framework crystalline material has good fluorescent property and stability, and can be applied to the field of fluorescence sensing.

The invention discloses a preparation method and application of a dual-channel multifunctional multicolor fluorescent probe, and relates to the technical field of fluorescent sensing detection.The dual-channel multifunctional multicolor fluorescent probe is prepared through the following steps that pyromellitic acid and ZrCl4 solids are dispersed in deionized water, Zr-MOF is prepared through a hydrothermal method, pamoic acid and melamine are dispersed in deionized water, and the Zr-MOF is prepared through a hydrothermal method; the preparation method comprises the following steps: preparing green fluorescent carbon dots (CDs) by using a hydrothermal method, dispersing the CDs and Zr-MOF in deionized water according to a certain proportion, reacting, collecting a brown solid, washing, drying, dispersing the solid in ethanol, adjusting the pH to be alkaline, adding Eu (NO3) 3.6 H2O, reacting, filtering, and drying in vacuum to obtain a target probe. The probe can accurately distinguish Cu < 2 + > ions of tetracycline antibiotics (TCs) through two independent fluorescent channels, not only displays completely independent spectral response, but also has the advantages of self-calibration, high sensitivity, interference resistance, field visual detection and the like. The multifunctional fluorescent probe has important significance for improving detection efficiency, reducing cost and energy consumption, optimizing resource utilization and promoting green low-carbon economy.

The invention provides a method for screening a hyaluronidase (HAase) inhibitor, and belongs to the technical field of medicines. The method comprises the following steps: firstly, rapidly identifying a natural product with HAase inhibition activity by adopting a fluorescence sensing technology according to the principle that carbon quantum dots (CDs) are used as a fluorescence probe, hyaluronic acid (HA) modified gold nanoparticles are used as a quencher, and the fluorescence of the CDs can be quenched by the quencher; when HAase exists, HA can be enzymatically decomposed to release CDs, and fluorescence recovery is caused; when a screened sample has HAase inhibition activity, CDs fluorescence recovery can be prevented. And 2, capturing components in a sample capable of inhibiting HAase activity by virtue of an affinity chromatography technology, immobilizing HAase on a carrier material by virtue of a method, eluting by virtue of a solvent to obtain components capable of being combined with HAase, and identifying the components by virtue of a liquid chromatography-mass spectrometry technology. According to the method, the HAase inhibitor can be rapidly and accurately screened from natural products.

The present invention discloses a method for detecting vitamin B1 using fluorescence sensing using single-stranded DNA gold nanoclusters. Using gold nanoclusters synthesized using single-stranded A30-ssDNA as a template as fluorescent probes, this method enables label-free, rapid, highly sensitive, and highly specific quantitative detection of vitamin B1 content in complex systems. Specifically, gold nanoclusters synthesized using single-stranded A30-ssDNA as a template serve as fluorescent probes, specifically detecting vitamin B1 content in a solution through changes in fluorescence intensity. This method is simple, rapid, and possesses a wide linear range, a low limit of detection, good sensitivity, and specificity, and has promising application prospects in food, pharmaceuticals, and other areas.

The invention discloses a preparation method of a portable paper-based ratiometric fluorescence sensor APBA-CsPbBr3 at-UiO-66 / Ru, which comprises the following steps: 1, adding lead bromide and cesium bromide into N, N-dimethylformamide, then adding 5-bromovaleric acid and an oleylamine ligand, and stirring; 2, perfluorooctyltriethoxysilane is added, and a precursor solution is obtained; 3, adding zirconium chloride and terephthalic acid into N, N-dimethylformamide to obtain a homogeneous solution; 4, after heating, UiO-66 is obtained; 5, stirring the precursor solution, APBA, UiO-66 and tris (2, 2-dipyridyl) ruthenium chloride hexahydrate to obtain a composite material; and 6, dropwise adding the composite material solution into the filter paper. The APBA modified material is adopted as a recognition unit, the APBA modified material and the paper base construct a fluorescence sensing platform, visual quantitative analysis of oxytetracycline in water is achieved, and the device is convenient to carry, low in detection limit, high in anti-interference performance, easy and convenient to operate and suitable for on-site rapid detection of oxytetracycline.

The present application relates to a fluorescence sensing composite deep electrode, belonging to the field of medical devices, and comprising: a tube body, on the outer part of which several annular electrodes are sequentially arranged along the axial direction, each annular electrode is connected with an electrode wire, and one end of the tube body is a closed end; several optical fiber sensors are also arranged on the tube body, the optical fiber sensors include a detection end and a transmission end, and the detection end is used for detecting the fluorescence generated when a fluorescent indicator acts on metal ions during the neuron activity process; the ends of the electrode wires far away from the annular electrodes and the transmission ends of the optical fiber sensors are led out from the end of the tube body far away from the closed end. The fluorescence sensing composite deep electrode provided by the present application can collect and monitor the electroencephalogram signals, metal ion concentration and distribution in the monitoring area in real time, synchronously and in the same position in the fields of neurosurgery and cerebrospinal nerve function research, and improves the monitoring accuracy of nerve activities and the diagnosis accuracy and efficiency of the lesions of nervous system diseases.

The invention provides a glucose hydrogel optical fiber sensor and a preparation method thereof. The glucose hydrogel optical fiber sensor comprises an optical fiber and a glucose fluorescence sensing module fixedly carried on the end face of the optical fiber. Wherein the optical fiber comprises a double-layer hydrogel optical fiber which comprises an inner fiber core and an outer cladding. The glucose fluorescence sensing module is formed by fixing a glucose fluorescence probe containing a boric acid group and a terminal amino group through a covalent reaction between the amino group and an epoxy group in the hydrogel network. The glucose hydrogel optical fiber sensor has excellent performance, high sensitivity and good selectivity, and can realize stable and real-time detection of glucose concentration in a complex physiological environment. The sensor is flexible in structure and good in biocompatibility, does not depend on enzyme reaction, and avoids the problem of signal drift caused by enzyme inactivation; meanwhile, the sensor is good in repeatability, can be integrated with other optical sensing modules to realize multi-parameter synchronous monitoring, and is suitable for long-term dynamic monitoring application in vivo.

The invention discloses a fluorescence sensing system based on nitrogen-doped carbon dots and machine learning and application of the fluorescence sensing system in recognition of benzenediol isomers, and belongs to the technical field of analytical chemistry and artificial intelligence. According to the invention, the undoped carbon dots and the nitrogen-doped carbon dots of which the properties change in a gradient manner are utilized to form the probe library, the carbon dots in the probe library can perform collaborative recognition, and weak differences between analytes are accumulated and amplified. Although the response difference of each single probe in a probe library to two similar isomers is very small, the tiny differences can be accumulated and amplified in a high-dimensional response vector due to the gradient change of the properties of the probes; the microscopic chemical property difference, which is difficult to directly measure, between the isomers is converted into the macroscopic high-dimensional data mode difference which can be easily identified by a machine, so that the accurate identification and classification of the structural similar substances and the mixture thereof are realized.

The invention discloses an application of a copper nano-cluster with stable ascorbic acid in detection of p-nitrophenol, the copper nano-cluster with stable ascorbic acid is used as a fluorescent probe, and the detection of p-nitrophenol is realized through a fluorescent sensing method. Due to the quenching effect of p-NP, the fluorescence intensity of the system is obviously reduced in the presence of p-NP. The p-NP concentration can be quantitatively detected by comparing the fluorescence intensity of the copper nano-cluster and the fluorescence intensity of a quenching system. The method is simple, sensitive and high in selectivity, does not need complex fluorescent dye labeling, shows remarkable potential for p-NP detection, and has a good application prospect in the aspects of food, medicine and the like.

The invention discloses a targeted design and optimization method for an organic pollutant fluorescence sensing array and a signal acquisition device. The overall information of the array is obtained in a high-throughput manner in a fluorescence imaging mode; based on an inner filter effect sensing mechanism, the model selection of a sensing material and an optical device is carried out by taking the absorption spectrum characteristic of a target organic detection object as a starting point, and sensing optimization is carried out based on the controllability of weak interaction between the sensing material and the target detection object. The use process comprises two aspects of sample library construction and modeling and detection application. The method comprises the steps of sensing material selection, model selection of an excitation light source and a signal acquisition device, light path arrangement design and sensing performance optimization. The detection device disclosed by the invention has proper detection precision and portability, can replace a precise fluorescence spectrophotometer, and realizes low-cost, high-throughput and real-time field detection of a water body.

This invention provides a method for screening β-galactosidase inhibitors, belonging to the field of pharmaceutical technology. The first step of this method uses fluorescence sensing technology to rapidly identify natural products with β-Gal inhibitory activity. The principle is based on the fluorescence quenching property of p-nitrophenol (PNP) on sulfur quantum dots (SQDs); β-Gal catalyzes the hydrolysis of p-nitrophenyl-β-D-galactopyranoside (PNPG) to generate the specific product PNP; in the presence of β-Gal, PNPG can be enzymatically catalyzed to generate PNP, quenching the fluorescence of SQDs; when screened for β-Gal inhibitory activity, the fluorescence of SQDs can be restored. The second step uses affinity chromatography to capture components in samples with β-Gal inhibitory activity. This method involves immobilizing β-Gal on a support material, eluting with a solvent to obtain components that can bind to β-Gal, and then using liquid chromatography-mass spectrometry (LC-MS) to identify the components. This method can rapidly and accurately screen β-Gal inhibitors from natural products.

The present invention relates to a fiber optic fluorescence sensing detection system for food pathogens, comprising a sensing module and a detection module. The sensing module includes a fiber optic fluorescence sensing module (optical components and a fluorescence reaction module) and an environmental sensing module. The optical components include an optical fiber array, a filter, a collimating lens, and an image sensor. The fluorescence reaction module includes an excitation light source and a multi-channel fluorescence reaction tank. The excitation light source irradiates the solution in the centrifuge tube in the multi-channel fluorescence reaction tank to stimulate a fluorescence signal. The fiber optic array transmits the fluorescence signal. The filter selects the fluorescence signal. The collimating lens amplifies and focuses the fluorescence signal. The image sensor is used to image the fluorescence signal. The environmental sensing module includes several sensors that collect environmental data. The detection module obtains a fluorescence intensity image obtained by the image sensor and environmental data collected by the environmental sensing module, and performs pathogen detection on the solution of the food sample. The present invention can effectively detect food pathogens.

The invention provides a copper halide coordination material with pressure stimulation response luminescence performance and a preparation method and application thereof, and belongs to the technical field of luminescent materials. The organic phosphine ligand is constructed by the functional organic phosphine ligand containing the furan or thiophene skeleton and the copper halide through coordination, has excellent structural stability and light-emitting response performance, particularly shows a remarkable optical regulation behavior under a pressure stimulation condition, and is suitable for the fields of high-pressure fluorescence sensing or strain sensing devices and the like. Various novel ligands are provided, corresponding copper halide compounds are systematically constructed, and the structure-function relationship between the material structure and the high-pressure optical response of the material structure is established.

This invention discloses a coumarin-linked conjugated porous polymer, its preparation method, and its applications. The preparation method includes: subjecting a homogeneous mixture containing o-hydroxybenzaldehyde monomer, phenylacetonitrile monomer, solvent, and catalyst to a Knauven-Gail condensation reaction to obtain the coumarin-linked conjugated porous polymer. The coumarin used in this invention to prepare the coumarin-linked conjugated porous polymer is a highly planar molecule. Its full conjugation extends the absorption wavelength to the visible light region and enhances π-electron excitation, thus exhibiting strong photosensitivity. The resulting series of coumarin-linked highly conjugated nanoarray conjugated porous polymers possess high porosity and a fully conjugated structure, making them highly efficient for applications in photocatalysis, fluorescence sensing, and optoelectronic devices.

The invention relates to the field of medical detection, in particular to a fluorescent sensing composition, a fluorescent sensing film and application thereof. According to the invention, by screening a plurality of fluorescent indicators and combinations thereof, an indicator formula with simple components and low cost is finally obtained. Based on the formula, by optimizing the preparation process, the high-efficiency fluorescent sensing film resistant to damp-heat sterilization is developed. After a microorganism culture bottle prepared on the basis of the membrane is inoculated with Gram-negative bacilli, positive cocci and yeast fungi representative quality control strains, experiments of full-automatic microorganism culture system fluorescence curve determination, Gram staining, microorganism mass spectrum identification and the like prove that the fluorescence sensing membrane is excellent in detection performance. The fluorescent sensing film provided by the research is applied to clinical microorganism detection, can provide technical support for blood flow infection diagnosis, and is of great significance in suppressing antibiotic abuse and improving prognosis of blood flow infection patients.

The present invention is applicable to the fields of stimulus-responsive light-variable materials, catalysis, fluorescence sensing, biomolecule labeling, information processing and storage, and anti-counterfeiting technology, and provides the synthesis and application of a concentration-solvent-dependent platinum complex luminescent material. The preparation process of the present method is simple and easy to purify. The obtained ionic metal Pt(II) complex phosphorescent material with concentration- and solvent-dependent luminescence changes is stimuli-responsive to solvent and concentration.

The application discloses a tryptamine detection method based on triple emission MOFs and a smart phone. A rare earth metal organic framework (AP-Eu) is used as a luminescent matrix, and a fluorescent guest molecule fluorescein-5-isothiocyanate (FITC) is post-modified to obtain a ratio fluorescent material with triple emission characteristics. Competitive absorption of ligands and tryptamine, pH response of FITC and hydrogen bonding are used to realize fluorescence signal regulation, and then high-sensitivity and high-selectivity fluorescence recognition of tryptamine is realized. The method is further applied to quantitative detection of tryptamine in beer samples, visualization detection of foam and on-site rapid detection assisted by a mobile phone APP. The application provides a safe and efficient tryptamine detection and fluorescence sensing method, and significantly improves detection accuracy and on-site applicability.

The invention discloses a self-feedback intelligent electrochemical wastewater treatment platform and a construction method thereof. The platform modularly integrates an electrochemical water treatment unit, a terephthalic acid probe molecular fluorescence sensing unit, a signal acquisition data processing unit and an electro-hydraulic linkage online control unit. All the units work cooperatively, and the electrochemical water treatment unit generates strong oxidizing hydroxyl radical mineralized pollutants; the fluorescence sensing and data processing unit is used for converting. OH specificity into a fluorescence signal by utilizing probe molecules and mapping the fluorescence signal into. OH steady-state equivalent concentration in real time; and the online control unit implements a model prediction control strategy based on concentration feedback, dynamically adjusts the current density, the electrode potential and the wastewater flow, and realizes real-time adaptive matching of the interface mass transfer and the. OH generation rate. According to the invention, electrogenerated. OH on-line monitoring and dynamic closed-loop optimization of operation parameters are realized, deep mineralization, low energy consumption and long-term stable operation are realized, and the method is suitable for engineering and intelligent operation of electrochemical advanced oxidation treatment of industrial wastewater.

The invention discloses a fluorescent pigment compound as well as a preparation method and application thereof. The chemical structural formula of the fluorescent pigment compound is shown in the specification. Wherein n is an integer from 2 to 6, and R1 and R2 are respectively and independently selected from C1-C3 alkyl groups. By introducing 4-tert-butylphenyl and tert-butylsilane ether chains, the compound has excellent solubility, film-forming property, high fluorescence quantum yield (gt, 95%) and light stability (retention rate gt, 90% after ultraviolet irradiation for 500 hours). The preparation method is simple, is suitable for a solution processing technology, and can be widely applied to the fields of OLEDs, light conversion adhesive films, fluorescence sensing, biological imaging and the like.

The invention discloses a nitrogen-doped carbon dot and a rapid fluorescence detection method thereof for indoxacarb residue, and belongs to the field of biosensor technology and food quality detection. L-theanine and ethidene diamine are used as raw materials, the nitrogen-doped carbon dots are prepared through a hydrothermal method, the N-CDs have the advantages of being stable in fluorescence emission, good in salt tolerance and excellent in photobleaching resistance, and indoxacarb can specifically quench fluorescence through the static quenching effect. By optimizing N-CDs synthesis conditions and indoxacarb detection conditions, an indoxacarb fluorescence sensing detection system with high selectivity and high sensitivity is constructed. The detection method disclosed by the invention can be suitable for detecting indoxacarb residues in environmental water samples such as tap water and river water and agricultural product matrixes such as apples and tea leaves, the standard recovery rate is good, the relative standard deviation is low, on-site rapid screening of the indoxacarb residues can be realized, and a new technical means is provided for monitoring the quality safety of the environment and agricultural products.

The present invention discloses a luminescent molecule based on a tetrakis(4-pyridylphenyl)ethylene core structure, a long-wavelength AIE array sensor, and a preparation method and application thereof. The TPPESA array sensor designed by the present invention can accurately identify and detect glycosaminoglycan with a concentration as low as 1 μg / mL. In terms of detecting a glycosaminoglycan mixture (impurity), the array sensor of the present invention can detect contaminants with a minimum content of 2% in a sample. The detection operation is simple, fast, and efficient. It can realize the detection of a variety of saccharide compounds: the fluorescence sensing array constructed by the present invention can be used for the quantitative, accurate, and rapid detection of common 4 glycosaminoglycans in a water sample and a serum environment. In addition, when the fluorescence sensing array of the present invention is used for detecting saccharide compounds, no harmful by-products are generated and there is no secondary pollution.

The application discloses a preparation method of a two-dimensional supramolecular polymer based on cation-pi interaction co-assembly, and relates to the technical field of supramolecular polymer materials. + As a cationic unit, a monomer molecule 4,4',4''-(3,7,11-trimethyltriphenylene-2,6,10-triyl)tris(N-dodecylbenzamide) is synthesized as a pi unit, and then the cationic unit and the pi unit are co-assembled to form a two-dimensional supramolecular polymer based on cation-pi interaction co-assembly. The prepared two-dimensional supramolecular polymer has fluorescence color change characteristics and photocatalytic hydrogen evolution performance, further widens the force category of two-dimensional supramolecular materials, and provides a new strategy for the preparation of two-dimensional supramolecular materials and the application of the two-dimensional supramolecular materials in the fields of fluorescence sensing, fluorescence anti-counterfeiting and photocatalytic hydrogen evolution.

The invention relates to a metal oxide sheet with spherical convex particles and oxygen vacancies enriched on the surface, a preparation method and application. According to the sheet preparation method, metal oxide sheet xerogel is obtained through a heating bubbling method, and spherical convex particles and oxygen defects are enriched on the surface of the metal oxide sheet xerogel in combination with a staged annealing oxidation process. The diameter of the spherical convex particles on the surface of the thin sheet is 50-100 nm, the thickness of the thin sheet is 100-500 nm, the length and width size range of the thin sheet is not limited, and the thin sheet is suitable for various size specifications. The surface of the thin sheet is enriched with spherical convex particles and oxygen defects, so that adsorption sites with relatively high surface area / volume and high density can be provided, the surface electronic structure is improved, the electron transmission rate is accelerated, the sensing performance is enhanced, and the sensing stability is improved. The thin sheet has a wide application prospect in the fields of gas sensing, colorimetric sensing, fluorescence sensing, stress sensing, chemical sensing, photoelectric sensing and biological sensing.

The invention belongs to the technical field of fluorescence sensing, and particularly relates to a fluorescence analysis method which comprises the following steps: mixing a solution to be detected with chromium selenide quantum dots, and detecting the fluorescence intensity of a system to obtain the content of sodium tripolyphosphate in the solution to be detected. According to the present invention, it is found that sodium tripolyphosphate can selectively quench the fluorescence of chromium selenide quantum dots, such that the chromium selenide quantum dot-based sodium tripolyphosphate fluorescence analysis method is provided so as to meet the requirement of efficient detection of sodium tripolyphosphate.

The invention relates to the technical field of fluorescence sensing and colorimetric detection, in particular to a concentration-gated three-emission fluorescence-colorimetric dual-mode visual sensing probe as well as a preparation method and application thereof. A three-emission fluorescence system is combined with a colorimetric signal, multiple reading channels are provided, the lower detection limit is remarkably lower than that of a traditional single-signal sensor, and good linear response is kept in a wide concentration range. Fluorescence and colorimetric modes are based on different mechanisms and are mutually independent and complemented, so that false positive / negative is effectively avoided, and the reliability of a detection result is remarkably improved. The continuous change of the fluorescence color from blue to red to orange to yellow to green can be identified by naked eyes or a smart phone, and the method is suitable for on-site rapid detection and semi-quantitative analysis. A concentration gating mechanism enables the sensor to present a switching type response behavior in a low / high concentration interval, a saturation phenomenon is avoided, a detection dynamic range is expanded, and the sensor is especially suitable for synchronous monitoring of trace and excessive residues of TC.

The invention discloses a fluorescence sensing system based on machine learning and carbon dots and application thereof, and belongs to the technical field of analytical chemistry, the fluorescence sensing system comprises a sensing array unit, a data acquisition unit and a data processing unit; the sensing array unit comprises more than three types of sensing units, and the more than three types of sensing units are obtained by respectively placing a single type of fluorescent probe in different chemical environment media; the data acquisition unit is used for acquiring a fluorescence signal of each sensing unit to form a data matrix; and the data processing unit is internally provided with a machine learning model and is used for processing the data matrix and outputting a recognition result of the metal ions. The invention provides a technical method of sensor array construction based on a single probe, signal diversity is created by regulating and controlling an external environment instead of an internal structure of the probe, the technical method is a brand-new technical means with remarkable economical efficiency and simplicity, and the technical method is essentially different from the prior art relying on synthesis of multiple probes.