286 results about "Fluorescence sensing" patented technology

The invention relates to the technical field of fluorescence sensing development, in particular to a Cd (II)-based metal organic framework crystalline material as well as a preparation method and application thereof. The chemical formula of the Cd (II)-based metal organic framework crystalline material is {[Cd3 (H2O) 2 (DMA) 2 (5-CMOIA) 2]. 6 / 5H2O} n, the organic framework material is characterized by an X-ray single crystal diffractometer, and the result shows that the organic framework material is crystallized in a triclinic system P-1 space group, the asymmetric unit of the organic framework material is composed of three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions and three The complex is composed of two deprotonated (5-CMOIA) 3-ligands, two coordinated DMA solvent molecules, two coordinated water molecules and six fifths of crystal water molecules. The solid fluorescence spectrum of the Cd (II)-based metal organic framework crystalline material is measured, the fluorescence response of the Cd (II)-based metal organic framework crystalline material to metal ions in a solution is explored, and the result shows that the Cd (II)-based metal organic framework crystalline material can effectively recognize Hg < 2 + > from different ions and can be applied to the field of fluorescence sensing.

The invention provides a ratio type molecular imprinting fluorescence sensing detection method based on a metal organic framework and nitrogen-doped carbon dots, the advantages of N-CDs, Eu-MOF and a molecular imprinting layer are fused, in the presence of target molecules, the blue fluorescence (430nm) of the N-CDs is effectively enhanced due to non-radiative recombination caused by the surface defect state of the CDs, and the red fluorescence (660nm) of the Eu-MOF remains unchanged. According to the ratio type molecularly imprinted fluorescent sensing detection method, while a fluorescent response signal is displayed, the color of the imprinted polymer changes along with different concentrations of the target molecules, and sensitive visual detection of the target molecules can be realized, so that specific sensitive detection of the target molecules in a complex sample is realized; the method has the advantages of high sensitivity, simplicity and convenience in operation, good reproducibility, strong anti-interference performance and the like. According to the method, rapid and sensitive detection of organophosphorus pesticides, toxic agents and other imprinted molecules can be realized by preparing imprinted polymers of different target molecules without fluorescence interference, and the method has a wide application prospect.

The invention relates to the field of physiological status detection, and provides a wearable microfluidic fluorescence sensor based on a Janus membrane for sweat analysis. The sensor comprises a Janus membrane-based micro-fluidic chip and a fluorescent sensing layer which are stacked, the Janus membrane-based micro-fluidic chip comprises a hydrophobic nanofiber membrane, a bottom polydimethylsiloxane layer, a hydrophilic nanofiber membrane and a top polydimethylsiloxane layer which are stacked, and the fluorescent sensing layer comprises three paper chips, the simultaneous fluorescence detection of chloride ions, glucose and pH of sweat is realized. The Janus membrane with the directional water conveying capacity is used as a substrate for preparing a micro-fluidic chip. In one aspect, sweat may be automatically delivered to an analysis zone along a designed path. On the other hand, sweat is transferred from a hydrophobic membrane close to the skin to a hydrophilic membrane, sweat accumulation is effectively avoided, and a comfortable skin microenvironment is promoted. A Janus membrane-based micro-fluidic chip is combined with fluorescence sensing, a micro-fluidic fluorescence sensor based on a Janus membrane is constructed, and simultaneous high-stability and high-accuracy analysis of multiple targets in human sweat is realized.

The invention discloses a CDs / QDs (at) ZIF-8 probe, a preparation method of the CDs / QDs (at) ZIF-8 probe and application of the CDs / QDs (at) ZIF-8 probe to detection of tetracycline at three emission ratios, and belongs to the technical field of fluorescence sensing. The probe comprises ZIF-8 as well as a carbon dot and a cadmium telluride quantum dot which are encapsulated in the ZIF-8. The preparation method comprises the following steps: sequentially adding CDs and QDs into a 2-methylimidazole solution, uniformly mixing, dropwise adding a zinc salt solution, and reacting to obtain the CDs / QDs ZIF-8 probe. The probe can be used for performing three-emission-ratio fluorescent quantitative detection on tetracycline, and the detection limit is 14.2 nmol / L. The method is simple in synthesis steps and easy in condition control. The fluorescent color of the mixed solution of CDs / QDs ZIF-8 and tetracycline is changed from powder to green along with the increase of the concentration of tetracycline, the detection method is visual and rapid, the visualization effect is good, the detection gel can be prepared, the detection gel can be combined with a smartphone for use, and the method is simple, convenient to carry and convenient for field judgment.

The invention relates to the technical field of fluorescence sensing, in particular to a preparation method and application of an Nd (III)-based hydrogen bond organic framework crystalline material. According to the Nd (III)-based hydrogen bond organic framework crystalline state material, Nd (III) serves as a central metal ion and is coordinated with 9 oxygen atoms, and adjacent Nd (III) are connected through H2 (4-CPCA) ligands to form a one-dimensional chain structure. One-dimensional chains are bridged through H2 (4-CPCA) ligands to form a two-dimensional double-layer planar structure, and two-dimensional surfaces are further stacked through the interaction of a large number of intermolecular hydrogen bonds to form a three-dimensional supramolecular structure. The Nd (III)-based hydrogen bond organic framework crystalline material has good fluorescent property and stability, and can be applied to the field of fluorescence sensing.

The invention discloses a method for distinguishing similar antibiotics by using a fluorescent sensing array based on YOLOv5 target recognition. The method comprises the following steps: S1, preparing GMP-Tb solid powder; s2, mixing the GMP-Tb solid powder with a rhodamine B solution in proportion to obtain a multi-proportion fluorescent probe; s3, mixing antibiotics with different concentrations with the multi-proportion fluorescent probe to obtain a fluorescent sensing array; s4, collecting color images in the fluorescent sensing array and dividing the color images into a training set and a test set; s5, constructing an OLOv5 optic nerve model, inputting the acquired color image into a YOLOv5 optic nerve model, and outputting a feature value of the acquired color image; s6, inputting the characteristic value of the color image into a classifier model for prediction classification to obtain a distinguishing result; and S7, inputting a color image formed by mixing the to-be-detected antibiotic and the multi-proportion fluorescent probe into the YOLOv5 optic nerve model to obtain a characteristic value of the color image, and then outputting a distinguishing result through the classifier model.

The present invention combines a fluorescence sensor with an array to provide a fluorescence sensing array detection platform and a detection method for multiple plasticizers. The fluorescence sensing array detection platform includes a B-doped graphene quantum dot solution using N,N-dimethylformamide as a solvent and a B-GQDs solution using cyclohexane as a solvent; the fluorescence intensity of the B-GQDs solution with DMF as the solvent at the fluorescence characteristic peak obtained under ultraviolet light excitation at a wavelength of 350 nm, and the fluorescence data at 391 nm, 413 nm, and 434 nm are used as array points 1, 2, and 3; the fluorescence intensity of the B-GQDs solution with CH as the solvent at the fluorescence characteristic peak obtained under ultraviolet light excitation at a wavelength of 350 nm, and the fluorescence data at 386 nm, 408 nm, and 430 nm are used as array points 4, 5, and 6; thus, a 3×2 fluorescence sensor array is constructed. The detection method using the fluorescence sensor array of the present invention has simple steps, and has satisfactory specificity, reproducibility, stability and practicability, and has application potential.

The invention discloses a europium-based metal-organic framework as well as a preparation method and application thereof, and belongs to the technical field of bioassay materials. The preparation method of the europium-based metal-organic framework comprises the following steps: adding europium salt, H4BTA-2OH and ascorbic acid into a binary solution, and sequentially performing ultrasonic treatment, heating and post-treatment to prepare the europium-based metal-organic framework (the molecular formula is {[(CH3) 2NH2] [Eu (BTA-2OH) (H2O) 2]. 4H2O} n). In addition, the invention also discloses an application of the europium-based metal-organic framework in detection of a bacillus anthracis biomarker 2, 6-dipicolinic acid (DPA). The europium-based metal-organic framework Eu-MOF prepared by the method can rapidly detect 2, 6-dipicolinic acid, and has high selectivity and sensitivity, and the excellent fluorescence sensing ability means that the europium-based metal-organic framework Eu-MOF is a potential bacillus anthracis detection material.

The invention discloses an azodicarbonamide ratio fluorescence detection method based on a CoOOH nanosheet loaded Au nano-cluster material, and belongs to the technical field of fluorescence sensing. The CoOOH nanosheet loaded Au nano-cluster material provided by the invention is prepared by carrying out a one-step electrostatic assembly method on AuNCs and a CoOOH nanosheet. The CoOOH nanosheet loaded Au nano-cluster material has the fluorescence property of the AuNCs and the biomimetic catalytic performance of the CoOOH nanosheet at the same time. The invention further provides an azodicarbonamide ratio fluorescence detection method based on the CoOOH nanosheet loaded Au nano-cluster material, and compared with an existing detection method, the azodicarbonamide ratio fluorescence detection method has the remarkable advantages that the method is high in selectivity; by utilizing a ratio fluorescence mechanism, interference caused by environment and instrument fluctuation can be effectively reduced, reliable quantitative analysis of azodicarbonamide is realized, and the method has the advantages of high sensitivity and high credibility and has a wide prospect in the field of fluorescence sensing detection.

The invention provides a ratiometric fluorescence sensing system and a preparation method and application thereof, and the preparation method comprises the following steps: respectively preparing a silicon oxide-based aggregation-induced emission material and a copper-doped carbon dot material, and mixing the silicon oxide-based aggregation-induced emission material and the copper-doped carbon dot material with an ascorbic acid-2-phosphoric acid material to construct the ratiometric fluorescence sensing system. The fluorescent probe is applied to ferric ion detection, in-vitro detection of biomarkers or fluorescence imaging. The technical scheme provided by the invention has good stability, accuracy and practical application potential.

The invention aims to disclose a preparation method and application of a probe Cu-HClO for detecting peroxynitrous acid. Peroxynitrous acid is used as an important physiological activation factor and signal molecule, and can regulate dynamic oxidation-reduction dynamic balance in a living body. Fluorescence sensing and fluorescence imaging can mark and track molecules with wide biological activity, and have good temporal and spatial resolution. Coumarin has unique fluorescence, and the fluorescence characteristic of coumarin depends on the structure of coumarin to a great extent, so that the modification of coumarin becomes a basis for constructing an efficient fluorescent probe. The novel fluorescent probe for detecting peroxynitrous acid is obtained on the basis of modifying a coumarin structure.

The invention discloses an aggregation-induced emission-metal-organic framework probe which is prepared by taking copper hydroxide as a metal center and 1, 1, 2, 2-tetra (4-carboxybenzene) ethylene as an organic ligand through a one-pot solvothermal method. The invention discloses a method for detecting diazinon by using a fluorescence sensing system constructed based on the aggregation-induced emission-metal-organic framework probe. The aggregation-induced emission-metal-organic framework probe is extremely low in background fluorescence, background signal interference during diazinon detection can be reduced, and sensitivity is improved. Meanwhile, enzyme mediation is introduced to serve as a controllable recognition element, specific and sensitive detection of diazinon is achieved, and the detection limit is 0.14 ng.mL <-1 >. The aggregation-induced emission-metal-organic framework probe disclosed by the invention shows the advantages of high sensitivity, high selectivity, quick response, stability, simplicity and convenience in operation and the like in diazinon detection, and has a wide application prospect.

The invention relates to the technical field of porous material preparation and detection of organic pollutants in a water phase, in particular to a fluorescent covalent organic framework material capable of being used for antibiotic detection and a preparation method and application thereof, and the covalent organic framework material is of a novel sql plane interspersed topological structure. The covalent organic framework material has a three-dimensional framework structure formed by interlocking rare sql two-dimensional planes, has fluorescent properties, is high in stability, can efficiently detect nitrofuran antibiotics in water through an electron transfer mechanism, and can be repeatedly used. According to the preparation method disclosed by the invention, a novel fluorescent covalent organic framework is designed and synthesized, and a covalent organic framework with a plane interpenetrating structure is synthesized and has a fluorescent property. The preparation method is easy to operate, environmentally friendly, low in cost and easy for industrial production. The prepared covalent organic framework is high in stability, and nitrofuran antibiotic pollutants in a water phase can be efficiently detected by combining a fluorescence sensing mechanism.

The invention relates to the technical field of fluorescence sensing, in particular to a praseodymium (III) metal organic framework crystalline material and a preparation method and application thereof. According to the praseodymium (III) metal organic framework crystalline state material, Pr (III) is used as a central metal ion and forms eight coordination with eight oxygen atoms of six (4-CPCA) 2-ligands. And adjacent Pr (III) are connected through 4-CPCA < 2-> bridging to form a one-dimensional annular chain. And the one-dimensional chains are further bridged and topologically connected through a (4-CPCA) 2-ligand to form a three-dimensional structure. The praseodymium (III) metal organic framework crystalline material has good fluorescent property and stability, and can be applied to the field of fluorescence sensing.

The invention relates to the technical field of fluorescence sensing, in particular to a Zn (II)-based metal organic framework crystalline material as well as a preparation method and application thereof. Zinc chloride and H3 (5-COIA) ligands are added into DMA, pH is adjusted, the Zn (II)-based metal organic framework material with a novel structure is prepared through a solvothermal method, the preparation method has the advantages of being convenient to operate, high in yield, good in reproducibility and the like, and the Zn (II)-based metal organic framework material has very strong fluorescence emission performance and good water stability and can be used for preparing a fluorescent material with a novel structure. It has been studied to selectively and sensitively detect La3 + ions in aqueous solutions, thereby further protecting human health and the environment from these contaminants.

The invention relates to a small molecular probe TPE-J based on fluorescence sensing and application thereof. According to the intelligent real-time monitoring system for the nitro explosive, a PPYOLO algorithm is utilized, and a fluorescent colorimetric image is captured under the assistance of an optical camera. The fluorescent probe TPE-J is designed and synthesized and is used for dose sensitivity and visual detection of nitro explosives. Electron transfer between PA and the probe causes specific response, original blue fluorescence is rapidly quenched to be non-luminous within 5 seconds, and the detection limit is as low as 1 mg / mL. Color changes can be integrated into optical camera capture and quantization, and generated image data is automatically processed by a deep learning algorithm platform. The sensing system is beneficial to efficient real-time monitoring and high-sensitivity PA detection in various scenes. A fluorescence sensing detection platform combined with deep learning provides a new visual angle for efficient and portable detection of explosives.

The invention relates to the technical field of fluorescence detection, in particular to a cadmium-metal-based fluorescent metal organic framework material, a preparation method of the cadmium-metal-based fluorescent metal organic framework material, a fluorescent sensor array and application of the cadmium-metal-based fluorescent metal organic framework material. Fluorescent probe molecules with different proportions can be obtained by changing the doping concentration of rhodamine B, the RhB (at) MOF has an emission peak of Cd-MOF at 430nm and an emission peak of rhodamine B at 570nm, and when the material is in contact with different benzene series solvents, the relative emission intensity of host-guest emission peaks is almost constant, and the material is unique for each RhB (at) MOF, so that the fluorescent probe can be used for detecting the fluorescence of the RhB (at) MOF. Benzene, toluene, xylene and ethylbenzene are specifically distinguished by using different relative emission ratios of host and guest emission peaks, a fluorescent sensing array is constructed to identify benzene series, and fluorescent probe molecules can be prepared into a fluorescent film to realize portable detection.

The invention discloses a method for simultaneously detecting bioavailability of adsorbed and desorbed antibiotics in soil, and belongs to the field of biotechnology application. A biosensing detection system is designed by adopting a method of combining fluorescence sensing bacteria and an agar plate, and the biosensing detection system is used for detecting the biosensing bacteria by controlling the humidity of an agar plate system based on soil-antibiotic interface behaviors and an agar diffusion principle. The system can detect and quantify the bioavailability of tetracycline adsorbed and desorbed in soil with different water potentials. The biosensing detection system designed by the invention is simple, sensitive, low in consumption and convenient, soil with the water potential range of-10.59 to-2.95 Kpa can be detected in situ, and the lowest detection limit value of tetracycline antibiotics is 1mg / kg.

The invention discloses a preparation method and application of a dual-channel multifunctional multicolor fluorescent probe, and relates to the technical field of fluorescent sensing detection.The dual-channel multifunctional multicolor fluorescent probe is prepared through the following steps that pyromellitic acid and ZrCl4 solids are dispersed in deionized water, Zr-MOF is prepared through a hydrothermal method, pamoic acid and melamine are dispersed in deionized water, and the Zr-MOF is prepared through a hydrothermal method; the preparation method comprises the following steps: preparing green fluorescent carbon dots (CDs) by using a hydrothermal method, dispersing the CDs and Zr-MOF in deionized water according to a certain proportion, reacting, collecting a brown solid, washing, drying, dispersing the solid in ethanol, adjusting the pH to be alkaline, adding Eu (NO3) 3.6 H2O, reacting, filtering, and drying in vacuum to obtain a target probe. The probe can accurately distinguish Cu < 2 + > ions of tetracycline antibiotics (TCs) through two independent fluorescent channels, not only displays completely independent spectral response, but also has the advantages of self-calibration, high sensitivity, interference resistance, field visual detection and the like. The multifunctional fluorescent probe has important significance for improving detection efficiency, reducing cost and energy consumption, optimizing resource utilization and promoting green low-carbon economy.

The intelligent real-time monitoring system for nitro explosives in the present disclosure adopts a PP-YOLO algorithm, and can capture. Combining fluorescence and colorimetric images with the assistance of an optical camera. The fluorescent probe TPE-J is designed and synthesized for the dosage-sensitive and visual detection of nitro explosives. The electron transfer between picric acid (PA) and the probe causes a specific response that the original blue fluorescence is rapidly quenched to non-luminescence within 5 s, with a detection limit as low as 1 mg / mL. A color change can be integrated into an optical camera for capture and quantization, and the resulting image data is automatically processed by a deep learning algorithm platform. The sensing system facilitates the efficient real-time monitoring and highly-sensitive detection of PA in various scenarios. The fluorescence sensing-based detection platform with deep learning provides a new perspective for the efficient portable detection of explosives.

The invention provides a method for screening a hyaluronidase (HAase) inhibitor, and belongs to the technical field of medicines. The method comprises the following steps: firstly, rapidly identifying a natural product with HAase inhibition activity by adopting a fluorescence sensing technology according to the principle that carbon quantum dots (CDs) are used as a fluorescence probe, hyaluronic acid (HA) modified gold nanoparticles are used as a quencher, and the fluorescence of the CDs can be quenched by the quencher; when HAase exists, HA can be enzymatically decomposed to release CDs, and fluorescence recovery is caused; when a screened sample has HAase inhibition activity, CDs fluorescence recovery can be prevented. And 2, capturing components in a sample capable of inhibiting HAase activity by virtue of an affinity chromatography technology, immobilizing HAase on a carrier material by virtue of a method, eluting by virtue of a solvent to obtain components capable of being combined with HAase, and identifying the components by virtue of a liquid chromatography-mass spectrometry technology. According to the method, the HAase inhibitor can be rapidly and accurately screened from natural products.

The invention provides a fluorescent probe for ratio type detection of biogenic amine as well as a preparation method and application of the fluorescent probe, and belongs to the technical field of fluorescent sensing. According to the A-D-A type organic fluorescent small molecular material, a conjugated structure containing a hydrogen bond binding site is adopted as a donor, an intramolecular charge transfer (ICT) state can be formed after an electron withdrawing group is introduced, and the purpose of fluorescence detection of biogenic amine is achieved through change of ICT properties before and after hydrogen bond interaction. The material has the advantages of high sensitivity, quick response, remarkable color change and reusability when being used for fluorescence detection of biogenic amines. Furthermore, a portable detection platform based on a smart phone is also constructed, and visual and quantitative detection of the food freshness is realized through digital analysis of the fluorescence image.

A method for concurrently measuring multiple biological parameters in an animal includes: a) illuminating using multiple illumination sources a region-of-interest of the animal that expresses a first genetically encoded fluorescent indicators (GEFI), a second GEFI, and a long-Stokes-shift (LSS) fluorescent compound that is insensitive to the multiple biological parameters; b) concurrently detecting fluorescence signals from the first GEFI, the second GEFI, and the LSS fluorescent compound using a multi-channel fluorescence sensing optical system; and c) processing the detected fluorescence signals to determine values of the multiple biological parameters, wherein the processing reduces instrument and / or biological artifacts in the values of the multiple biological parameters. The first and second GEFIs have distinct absorption spectra, where the first GEFI absorption spectrum overlaps the LSS fluorescent compound absorption spectrum, and the second GEFI emission spectrum overlaps the emission spectrum of the LSS fluorescent compound.

The invention aims to provide a preparation method and application of a green fluorescent carbon dot for detecting captopril, and belongs to the technical field of fluorescent carbon dots, diphenylamino-4-benzaldehyde and o-phenylenediamine are weighed and dissolved in ethanol, and ultrasonic treatment is performed to obtain a uniformly mixed solution; transferring the solution into a polytetrafluoroethylene reaction kettle, reacting for 2-8 hours at the temperature of 150-220 DEG C, standing and cooling to room temperature after the reaction is stopped, centrifuging to remove insoluble substances, taking supernate, and dialyzing for at least three days in a glass container through a dialysis bag of 500-1000Da to obtain a pure carbon dot aqueous solution; and freeze-drying the carbon dot aqueous solution to obtain a carbon dot solid. The prepared green fluorescent carbon dots can be used for detecting captopril, and are good in selectivity and high in sensitivity. Compared with other carbon dot-based fluorescence sensing methods, the method provided by the invention can be used for directly detecting captopril.

The invention belongs to the technical field of food detection, and relates to a method for identifying the producing area of tea based on a quantum dot fluorescent sensing array, which comprises the following steps: preparing InP / ZnS quantum dots with four different colors, preparing water-soluble functionalized fluorescent quantum dots by using the InP / ZnS quantum dots, constructing a four-channel fluorescent sensing array, measuring the fluorescence intensity of each tea water sample, and identifying the producing area of the tea based on the quantum dot fluorescent sensing array. Calculating the relative fluorescence intensity to obtain a fluorescence standard model spectrum of different types of tea leaves, taking to-be-detected tea leaves, obtaining a fluorescence model spectrum of the to-be-detected tea leaves, and comparing the fluorescence model spectrum with the fluorescence standard model spectrum to determine the production place of the to-be-detected tea leaves. By modifying the surface groups of the quantum dots, non-specific binding and cross response can be obtained, the construction of the quantum dot fluorescence sensing array is realized, the tea can be identified and distinguished through linear discriminant analysis, the complex pretreatment process of a tea sample is simplified, and the detection accuracy is improved. And operation is simple, cost is low, and large precise instruments are not needed.

The present invention discloses a method for detecting vitamin B1 using fluorescence sensing using single-stranded DNA gold nanoclusters. Using gold nanoclusters synthesized using single-stranded A30-ssDNA as a template as fluorescent probes, this method enables label-free, rapid, highly sensitive, and highly specific quantitative detection of vitamin B1 content in complex systems. Specifically, gold nanoclusters synthesized using single-stranded A30-ssDNA as a template serve as fluorescent probes, specifically detecting vitamin B1 content in a solution through changes in fluorescence intensity. This method is simple, rapid, and possesses a wide linear range, a low limit of detection, good sensitivity, and specificity, and has promising application prospects in food, pharmaceuticals, and other areas.

The invention discloses a preparation method of a portable paper-based ratiometric fluorescence sensor APBA-CsPbBr3 at-UiO-66 / Ru, which comprises the following steps: 1, adding lead bromide and cesium bromide into N, N-dimethylformamide, then adding 5-bromovaleric acid and an oleylamine ligand, and stirring; 2, perfluorooctyltriethoxysilane is added, and a precursor solution is obtained; 3, adding zirconium chloride and terephthalic acid into N, N-dimethylformamide to obtain a homogeneous solution; 4, after heating, UiO-66 is obtained; 5, stirring the precursor solution, APBA, UiO-66 and tris (2, 2-dipyridyl) ruthenium chloride hexahydrate to obtain a composite material; and 6, dropwise adding the composite material solution into the filter paper. The APBA modified material is adopted as a recognition unit, the APBA modified material and the paper base construct a fluorescence sensing platform, visual quantitative analysis of oxytetracycline in water is achieved, and the device is convenient to carry, low in detection limit, high in anti-interference performance, easy and convenient to operate and suitable for on-site rapid detection of oxytetracycline.

The present application relates to a fluorescence sensing composite deep electrode, belonging to the field of medical devices, and comprising: a tube body, on the outer part of which several annular electrodes are sequentially arranged along the axial direction, each annular electrode is connected with an electrode wire, and one end of the tube body is a closed end; several optical fiber sensors are also arranged on the tube body, the optical fiber sensors include a detection end and a transmission end, and the detection end is used for detecting the fluorescence generated when a fluorescent indicator acts on metal ions during the neuron activity process; the ends of the electrode wires far away from the annular electrodes and the transmission ends of the optical fiber sensors are led out from the end of the tube body far away from the closed end. The fluorescence sensing composite deep electrode provided by the present application can collect and monitor the electroencephalogram signals, metal ion concentration and distribution in the monitoring area in real time, synchronously and in the same position in the fields of neurosurgery and cerebrospinal nerve function research, and improves the monitoring accuracy of nerve activities and the diagnosis accuracy and efficiency of the lesions of nervous system diseases.

The present disclosure belongs to the technical fields of nanoscience and fluorescence sensing, and specifically relates to a preparation method of a paper-based sensor for detecting an ammonia gas, and a use of the paper-based sensor. In the present disclosure, Zn(PA)(BPE) is first prepared, and a porous structure on a surface of the Zn(PA)(BPE) is used to adsorb an ammonia gas to improve a reaction sensitivity of the ammonia gas; then a Zn(PA)@CNQD ratiometric fluorescent substance is prepared from Zn(PA)(BPE) and CNQD through embedding, and finally dissolved in ultrapure water (UPW) to obtain a solution; and the solution is added dropwise on a filter paper, and the filter paper is purged with nitrogen, such that the solution is loaded on the filter paper to obtain a ratiometric fluorescent paper-based sensor.

The invention provides a glucose hydrogel optical fiber sensor and a preparation method thereof. The glucose hydrogel optical fiber sensor comprises an optical fiber and a glucose fluorescence sensing module fixedly carried on the end face of the optical fiber. Wherein the optical fiber comprises a double-layer hydrogel optical fiber which comprises an inner fiber core and an outer cladding. The glucose fluorescence sensing module is formed by fixing a glucose fluorescence probe containing a boric acid group and a terminal amino group through a covalent reaction between the amino group and an epoxy group in the hydrogel network. The glucose hydrogel optical fiber sensor has excellent performance, high sensitivity and good selectivity, and can realize stable and real-time detection of glucose concentration in a complex physiological environment. The sensor is flexible in structure and good in biocompatibility, does not depend on enzyme reaction, and avoids the problem of signal drift caused by enzyme inactivation; meanwhile, the sensor is good in repeatability, can be integrated with other optical sensing modules to realize multi-parameter synchronous monitoring, and is suitable for long-term dynamic monitoring application in vivo.