20 results about "Fluorescence sensing" patented technology

This invention provides a method for screening β-galactosidase inhibitors, belonging to the field of pharmaceutical technology. The first step of this method uses fluorescence sensing technology to rapidly identify natural products with β-Gal inhibitory activity. The principle is based on the fluorescence quenching property of p-nitrophenol (PNP) on sulfur quantum dots (SQDs); β-Gal catalyzes the hydrolysis of p-nitrophenyl-β-D-galactopyranoside (PNPG) to generate the specific product PNP; in the presence of β-Gal, PNPG can be enzymatically catalyzed to generate PNP, quenching the fluorescence of SQDs; when screened for β-Gal inhibitory activity, the fluorescence of SQDs can be restored. The second step uses affinity chromatography to capture components in samples with β-Gal inhibitory activity. This method involves immobilizing β-Gal on a support material, eluting with a solvent to obtain components that can bind to β-Gal, and then using liquid chromatography-mass spectrometry (LC-MS) to identify the components. This method can rapidly and accurately screen β-Gal inhibitors from natural products.

This invention discloses a coumarin-linked conjugated porous polymer, its preparation method, and its applications. The preparation method includes: subjecting a homogeneous mixture containing o-hydroxybenzaldehyde monomer, phenylacetonitrile monomer, solvent, and catalyst to a Knauven-Gail condensation reaction to obtain the coumarin-linked conjugated porous polymer. The coumarin used in this invention to prepare the coumarin-linked conjugated porous polymer is a highly planar molecule. Its full conjugation extends the absorption wavelength to the visible light region and enhances π-electron excitation, thus exhibiting strong photosensitivity. The resulting series of coumarin-linked highly conjugated nanoarray conjugated porous polymers possess high porosity and a fully conjugated structure, making them highly efficient for applications in photocatalysis, fluorescence sensing, and optoelectronic devices.

This invention provides a supramolecular fluorescence sensing array and its application in detecting five tyrosine kinase inhibitors, belonging to the field of analytical chemistry detection technology. The supramolecular fluorescence sensing array is composed of three supramolecular fluorescent probes: 2NR@Q[8], 2AO@Q[8], and APFG@Q[8]. This invention uses a supramolecular fluorescence sensing array composed of three supramolecular fluorescent probes: 2NR@Q[8], 2AO@Q[8], and APFG@Q[8]. By utilizing the cross-fluorescence response signals generated by this supramolecular fluorescence sensing array for five TKIs, combined with classical pattern recognition algorithms, it is possible to perform qualitative and quantitative detection of five TKIs: GEF, EH, LD, AD, and DAC. Moreover, this supramolecular fluorescence sensing array has good anti-interference properties, and serum samples have demonstrated that the supramolecular fluorescence sensing array constructed in this invention can simultaneously analyze five TKIs in complex systems. Therefore, this invention provides a new method for detecting tyrosine kinase inhibitors, which is more intuitive and convenient than traditional detection techniques.

The application discloses a kind of preparation and application of N, P doped titanium carbide nano quantum dot fluorescent probe, belong to fluorescence sensing technical field.The application uses ethylenediamine, diammonium hydrogen phosphate as nitrogen source and phosphorus source respectively to synthesize the N, P doped Ti3C2MXene quantum dot of blue light emission, and the N, P doped Ti3C2MXene quantum dot has good stability and high quantum yield.Based on the fact that tetracycline (TC) can quench the fluorescence of N, P doped Ti3C2MXene quantum dot fluorescent probe, the N, P doped Ti3C2MXene quantum dot fluorescent probe is used for tetracycline detection.The fluorescent probe has high selectivity, wide linear range and high sensitivity, providing a new method for tetracycline detection in milk.

This invention discloses a gold nanocluster fluorescent sensing composite system, its preparation method, and its applications. Using ATT as both a protective and reducing agent, this invention synthesizes highly fluorescent gold nanoclusters ATT-AuNCs in one step; Eu is then added... 3+ A gold nanocluster fluorescence sensing composite system was constructed for high-sensitivity detection of TCs. Eu 3+ The fluorescence of ATT-AuNCs can be quenched. When TCs are added again, they competitively block Eu. 3+ The interaction with ATT-AuNCs restores the fluorescence of ATT-AuNCs, enabling the specific detection of TCs. This invention provides a novel technical approach for TC detection using a gold nanocluster fluorescent composite system. The preparation process is simple, the response speed is fast, and the anti-interference ability is strong.

This invention discloses an acridine-based fluorescent nanofilm, its preparation method, and its application in the fluorescence sensing detection of ethylenediamine, belonging to the field of gas phase sensing materials. The fluorescent nanofilm is prepared by the self-assembly reaction of an aldehyde-modified acridine derivative and calix[4]pyrroletetrahydrazine at the gas-liquid interface via acylhydrazone bonds. By controlling the precursor concentration and reaction time, this invention can achieve precise control of the nanofilm thickness. The prepared nanofilm has excellent self-supporting properties and a uniform porous network structure, effectively suppressing the aggregation-induced quenching effect. When this nanofilm is used as a fluorescent sensing material for the detection of ethylenediamine, it exhibits a ratiometric fluorescence response, with advantages such as fast response speed, rapid recovery, low detection limit, high selectivity, and good stability, which can meet the needs of rapid on-site visualization detection. The preparation method of this invention is simple, the reaction conditions are mild, and no template is required, which has important application prospects in the field of on-site visualization detection of ethylenediamine.

This invention proposes a quantum dot@ZIF-8 composite material, its preparation method, and its applications, belonging to the field of functional nanomaterials and fluorescence sensing analysis and detection technology. This invention involves mixing CdTe quantum dots (CdTe QDs) with red fluorescence emission and carbon quantum dots (C QDs) with blue fluorescence emission in a specific ratio, followed by in-situ self-assembly growth of ZIF-8, resulting in a quantum dot@ZIF-8 composite material with dual fluorescence signal emission. The quantum dot@ZIF-8 nanocomposite material prepared by this method not only possesses the fluorescence properties of quantum dots but also largely retains the original structure and properties of ZIF-8, making it a simple and effective method for preparing composite materials with excellent fluorescence performance. This quantum dot@ZIF-8 composite material can be used to prepare ratiometric fluorescence sensing probes for the visual detection of glucose with high sensitivity.

This invention belongs to the field of fluorescence detection and discloses a method for screening the spore biomarker pyridine dicarboxylic acid and the Eu used therein. 3+ -GQDs@MXene ratiometric fluorescent sensing material, which uses Ti3C2T x MXene nanosheets were used as a substrate to load graphene quantum dots and coordinate Eu. 3+ A composite material is formed. GQDs and MXene are prepared separately, then compounded and Eu is introduced. 3+ During detection, the sample to be tested is brought into contact with the sensing material, and 2,6-pyridine dicarboxylic acid is qualitatively or quantitatively detected based on the change in fluorescence intensity ratio at 420 nm and 615 nm. This method can be used for spore biomarker screening.

The application provides a fluorescent probe for biological amine ratio type detection and a preparation method and application thereof, and belongs to the technical field of fluorescence sensing. The A-D-A type organic fluorescent small molecule material of the application adopts a conjugated structure containing a hydrogen bond binding site as a donor, and can form an intramolecular charge transfer (ICT) state after introducing an electron-withdrawing group. The purpose of fluorescence detection of biological amine is achieved by the change of ICT properties before and after the hydrogen bond action. The fluorescence detection of biological amine by the material has the advantages of high sensitivity, rapid response, significant color change and reusability. Further, the application also constructs a portable detection platform based on a smart phone. Through digital analysis of the fluorescence image, the visual and quantitative detection of food freshness is realized.

This invention relates to the field of fluorescence sensing materials technology, specifically to a biporous cationic covalent organic framework material, its preparation method, a ratiometric fluorescent probe, and its applications. The unit cell parameters of the biporous cationic covalent organic framework material are: a = 72.3305 Å, b = 72.3305 Å, c = 3.7877 Å, α = β = 90.000°, γ = 120.000°; its structure is shown in Formula I. This invention utilizes the strong electrostatic interaction between the nanoscale pore wall ion sites of the biporous cationic covalent organic framework material and the charged groups of fluoroquinolone antibiotics, combined with a spatial matching effect, to achieve highly selective recognition of FQs.

The invention relates to the technical field of fluorescence sensing and food safety detection, in particular to a carbon quantum dot for mercury ion fluorescence detection and a preparation method and application thereof.According to the method, 2, 3-diaminopyridine serves as a C source and an N source, boric acid serves as a boron source, and N and B synergistically doped carbon quantum dots are synthesized in one step through a microwave method; a fluorescence sensing system with specific recognition capability on Hg < 2 + > is constructed, compared with traditional blue light carbon quantum dots, the carbon quantum dot has the emission wavelength of 425-520 nm, belongs to a long wavelength region, has the linear range of 0-25 [mu] mol / L, and can meet the detection requirements of trace-to-trace Hg < 2 + >; the detection limit of mercury ions (Hg < 2 + >) is as low as 32.8 nmol / L, which is far lower than the allowable concentration of Hg < 2 + > in food specified in food safety standards, and the problems of low sensitivity and poor stability of Hg < 2 + > detection based on carbon quantum dots in the prior art are solved.

The present application relates to a kind of based on self-adapting detection system and method of unmodified optical fiber probe and spectrum-image fusion, belong to optical fiber fluorescence sensing technical field.The system includes excitation light source, unmodified optical fiber probe, dual-mode signal acquisition unit and signal processing unit.The probe is immersed in the liquid to be measured to excite and collect fluorescence signal;The acquisition unit synchronously obtains fluorescence spectrum signal and fluorescence image signal;The processing unit uses the fusion prediction model based on the range-aware adaptive fusion stacking ensemble (RAAFSE) framework to process dual-mode signal, realizes the quantitative prediction of target concentration.The method simplifies sensor preparation by unmodified probe, improves data dimension using the information complementarity of spectrum and image, and realizes high-precision detection in wide concentration range with the aid of the adaptive fusion capability of RAAFSE framework.The system has high integration, good portability, and can be applied to rapid detection of various target substances by replacing fluorescent indicator.

The application relates to a kind of O-phosphorylated amino acid rapid ratio fluorescence detection methods, belong to phosphorylated amino acid detection technical field.The O-phosphorylated amino acid rapid ratio fluorescence detection method includes: adding alkaline phosphatase in the sample to be measured to carry out enzymolysis reaction, after enzymolysis reaction, EuTb-DPA MOF is added to carry out fluorescence sensing reaction, after fluorescence sensing reaction, the characteristic green fluorescence at 544.6 nm and the characteristic red fluorescence of 616.8 nm are detected, and ratio fluorescence signal is formed.The application is based on the cascade mechanism of "enzymolysis-position exchange-double emission response", and for the first time, the high selectivity, rapid (about 15 minutes) ratio fluorescence quantitative detection (red light 616.8 nm / green light 544.6 nm) of O-phosphorylated amino acid in serum, cell lysate and other various biological samples is realized.

The application discloses a water vapor-mediated solid-phase perovskite anion exchange method and application, and belongs to the technical field of fluorescence sensing. The water vapor-mediated solid-phase perovskite anion exchange method is characterized in that water vapor is used to mediate anion exchange between solid CsPbX3 and Cs4PbX6 perovskite nanocrystals on a paper substrate for the first time, and the fluorescence color change range of anion exchange between fluorescent CsPbBrI2 and non-fluorescent Cs4PbBr6 perovskite is the largest, and the fluorescence color change degree within a certain time range is positively correlated with the environmental humidity, so that the application has a wide application prospect.

This invention belongs to the field of in-situ imaging and fluorescence sensing technology for environmental processes, and particularly relates to a method for preparing a fluorescent thin film for in-situ imaging of the spatial distribution of reactive oxygen species (ROS) in sediments or soil. The method includes the following steps: dissolving 2',7''-dichlorodihydrofluorescein diacetate in an organic solvent to prepare a 2',7''-dichlorodihydrofluorescein diacetate stock solution; diluting the 2',7''-dichlorodihydrofluorescein diacetate stock solution with a buffer solution and performing a hydrolysis reaction to obtain a DCFH stock solution containing 2',7''-dichlorodihydrofluorescein; immersing a porous polymer film in the DCFH stock solution and performing saturation fixation under light-protected conditions. This method allows the prepared fluorescent thin film to be directly applied to the environment under test, avoiding destructive sampling and pretreatment of sediment / soil samples. It maximizes the preservation of the original structure and chemical environment of the sample, thereby obtaining more realistic and accurate in-situ ROS distribution information.

This invention belongs to the fields of photoexcitation device fabrication, solid waste recycling, and graphene powder technology. It provides a method for preparing iron-doped carbon dot composite materials for photoexcitation devices using coal gasification slag as raw material, and a device using the obtained carbon dots as a gain medium. The method involves washing and settling the coal gasification slag, combined with magnetic separation to screen out carbonaceous components with high iron content. The iron-doped carbon dot composite material is then obtained through steps such as oxidation, depolymerization, and exfoliation with hydrogen peroxide solution, followed by condensation and reflux, filtration, and drying. Specific pretreatment of the raw material enhances the particle size and fluorescence performance of the fluorescent carbon dots. By adjusting selective oxidation, depolymerization, and exfoliation conditions such as hydrogen peroxide concentration and temperature, the solid waste raw material can be used to prepare iron-doped carbon dot composite materials. The carbon nanoparticles prepared by this invention, after further modification, can be applied to fields such as photoamplification devices or fluorescence sensing.

The application belongs to the technical field of chemical sensing and environmental detection, and discloses a rare earth metal-organic framework, a preparation method thereof and application of the rare earth metal-organic framework in nitrophenol detection, the molecular formula of the rare earth metal-organic framework is {[Ln3(PZDC)4(Lac)(H2O) m (DMF) 4‑m ]·pDMF·qH2O} n wherein m is a constant between 0 and 4, p and q are constants between 0 and 6, n is an arbitrary natural number, Ln is samarium, europium, gadolinium, terbium ions and any proportion doping, PZDC is 3,5-pyrazole dicarboxylic acid anion, Lac is lactic acid anion, and DMF is N,N'-dimethylformamide. The rare earth metal-organic framework has high efficient fluorescence sensing performance, ultra-trace detection capability, rapid response, good cycle stability and excellent anti-interference capability.

This invention relates to a europium-based metal-organic framework material constructed based on 4-((3-(pyrazin-2-yl)-1H-pyrazol-1-yl)methyl)benzoic acid ligand, its preparation method, and its applications. The invention pertains to the fields of metal-organic framework materials, luminescent functional materials, and fluorescence sensing technologies. The chemical formula of the europium-based metal-organic framework material prepared by this invention is: [Eu(ppmb)2(NO3)(H2O)2] n ; where ppmb is the ligand after deprotonation of Hppmb; the structure of Hppmb is; the europium-based metal-organic framework material belongs to the triclinic crystal system, space group P-1, and unit cell parameters are a=9.727(4) Å, b=14.197(5) Å, c=25.615(9) Å, α=77.899(1)°, β=86.231(1)°, γ=70.044(1)°, and it is used in the detection of furazolidone.