Fluorescent silver nucleinate as well as preparation method and application thereof

Abstract

The invention belongs to the field of metal ion detection and nucleic acid reagent preparation and in particular relates to a fluorescent nanosilver nucleinate cluster as well as a preparation method and application thereof. A novel fluorescent DNA nanosilver cluster is synthesized by taking micromolecular nucleic acid as a template; the nanosilver cluster is 2-4nm in size; a water solution of the nanosilver cluster is light yellow, the nanosilver cluster is excited by red light of 600nm, relatively strong fluorescent light is emitted at the position of 660nm, the fluorescence quantum yield is 0.2 (compared with bipyridine ruthenium), fluorescent light at the position of 660nm can be quenched by trivalent and divalent iron ions, fluorescent light of DNA-AgNCs has remarkable different fluorescent inductions on trivalent and divalent iron ions, and the sensitivity on divalent iron is much higher than the sensitivity on trivalent iron, so that the total amount of trivalent and divalent iron can be qualitatively and quantitatively analyzed and low-concentration divalent iron (10<-9> to 10<-5> mu M) and trivalent iron (10<-6> to 10<-1> mu M) can be qualitatively analyzed.

Description

technical field [0001] The invention belongs to the field of metal ion detection and preparation of nucleic acid reagents, in particular to a fluorescent nano-nucleic acid-silver cluster and its preparation method and its application in rapidly detecting the content of divalent and ferric iron in a solution by fluorescence spectroscopy. Background technique [0002] In recent years, the use of artificially synthesized probes to selectively sense and identify important ions in the environment and in biological systems, especially heavy metal ions, has aroused great interest; iron ions are widely used in industrial production, and it is also a human body. One of the essential trace elements, iron participates in the transportation and storage of oxygen, and can also promote development; increase resistance to diseases; regulate tissue respiration, prevent fatigue; form hemoglobin, prevent and treat anemia caused by iron deficiency; The skin recovers good blood color; although ...

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Problems solved by technology

[0002]In recent years, the use of artificially synthesized probes to selectively sense and identify important ions in the environment and biological systems, especially heavy metal ions, has attracted great attention. Interest; iron ions are widely used in industrial production, and it is also one of the essential trace elements for the human body. Iron participates in the transportation and storage of oxygen, and can also promote development; increase resistance to diseases; regulate tissue respiration and prevent fatigue; composition Heme prevents and treats anemia caused by iron deficiency; restores good blood color to the skin; although iron (iron food) is an essential trace element (trace element food) for the human body, iron itself is not toxic, but when ingested Excessive or mistaken intake of excessive iron preparations may also lead to iron poisoning, heart and liver diseases, diabetes, etc. Therefore, it is particularly important to detect iron ions easily and quickly in the environment and in biological systems; in recent years, spectrophotometry has been adopted Reported for the detection of iron ions in solution [Wang Airong, Gu Yongqing, Wang Shaoleng. Overview of spectrophotometric determination of trace iron [J]. Guangdong Trace Element Science, 2005, 12(9): 5-10], but these methods are difficult to be accurate Measuring metal ions in cells and cannot distinguish ferrous ions and ferric ions well; fluorescent probes are widely used in biological and environmental detection, and the high sensitivity and high selectivity of fluorescent probes make it a An indispensable method in chemical analysis; silver nanoclusters using DNA as a template are gradually emerging because of their good biocompatibility and low toxicity [Zhao T T, Chen Q Y, Zeng C, Lan Y Q, Cai J G. Multi- DNA–Ag nanoclusters: reassembly mec hanism and sensing the change of HIF in cells [J]. J. Mater. Chem. B, 2013, 1(36): 4678-4683.; Obliosca J M, Liu C, Yeh H C. Fluorescent silver nanoclusters as DNA probes [J]. Nanoscale, 2013, 5(18): 8443-8461.]; the interaction between fluorescent silver nanoclusters of nucleic acid and special target substances will cause the change of fluorescence intensity, which is the qualitative identification of iron ions And quantitative detection provides a new idea. The difference between the use of nucleic acid silver for trivalent and ferrous iron ions has not been reported internationally. Our research will provide a new method for the rapid identification of iron ion species in organisms

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