Fluorescent silver nucleinate as well as preparation method and application thereof

A fluorescent nucleic acid silver, molecular nucleic acid technology, used in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve iron poisoning, heart and liver diseases, diabetes, difficult to accurately measure metal ions, and cannot be well differentiated Problems such as divalent iron ions and trivalent iron ions

Inactive Publication Date: 2014-12-03
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002]In recent years, the use of artificially synthesized probes to selectively sense and identify important ions in the environment and biological systems, especially heavy metal ions, has attracted great attention. Interest; iron ions are widely used in industrial production, and it is also one of the essential trace elements for the human body. Iron participates in the transportation and storage of oxygen, and can also promote development; increase resistance to diseases; regulate tissue respiration and prevent fatigue; composition Heme prevents and treats anemia caused by iron deficiency; restores good blood color to the skin; although iron (iron food) is an essential trace element (trace element food) for the human body, iron itself is not toxic, but when ingested Excessive or mistaken intake of excessive iron preparations may also lead to iron poisoning, heart and liver diseases, diabetes, etc. Therefore, it is particularly important to detect iron ions easily and quickly in the environment and in biological systems; in recent years, spectrophotometry has been adopted Reported for the detection of iron ions in solution [Wang Airong, Gu Yongqing, Wang Shaoleng. Overview of spectrophotometric determination of trace iron [J]. Guangdong Trace Element

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  • Fluorescent silver nucleinate as well as preparation method and application thereof
  • Fluorescent silver nucleinate as well as preparation method and application thereof
  • Fluorescent silver nucleinate as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 (best synthesis condition):

[0019] DNA and AgNO 3 The solution was mixed in sodium citrate buffer (pH 5, 10 mM), DNA and Ag +The concentrations were 6.4 and 200 μM, respectively. Transfer the mixed solution to a water bath for heating. The temperature rises to the Tm value of DNA (75°C) and stabilizes for three minutes, then slowly cools down to room temperature for 3.5 hours. 4 - :Ag + =2:1 add NaBH 4 (10 mM) solution, mix well and store in the dark at room temperature for 10 minutes, then transfer to 4 ℃ and store in the dark for 36 hours, the final volume of the reaction solution is 1 mL; prepare 2 μM, 1 μM, 0.5 μM DNA-AgNCs aqueous solution, Excited with 600 nm red light, the measured fluorescence intensity at 660 nm was 135, 72, and 40, respectively.

Embodiment 2

[0021] DNA and AgNO 3 The solution was mixed in sodium citrate buffer (pH 5, 10 mM), DNA and Ag + The concentrations were 6.4 and 100 μM respectively, and the mixed solution was transferred to a water bath for heating. After raising the temperature (70 ℃), it was stable for three minutes, and then slowly cooled to room temperature. The cooling process took 6 hours; 4 - :Ag + =1.5:1 add NaBH 4 (10 mM) solution, mix well and store in the dark at room temperature for 10 minutes, then transfer to 4 ℃ and store in the dark for 30 hours, the final volume of the reaction solution is 1 ml; prepare 2 μM, 1 μM, 0.5 μM DNA-AgNCs aqueous solution, and use Excited with 600 nm red light, the measured fluorescence intensity at 660 nm was 70, 34, and 15, respectively.

Embodiment 3

[0023] DNA and AgNO 3 The solution was mixed in sodium citrate buffer (pH 5, 10 mM), DNA and Ag + The concentrations were 6.4 and 200 μM respectively, and the mixed solution was transferred to a water bath for heating. After the temperature rose to 80 °C, it was stable for three minutes, and then slowly cooled to room temperature. The cooling process lasted for 4 hours; 4 - :Ag + =1:1 add NaBH 4 (10 mM) solution, mix well and store in the dark at room temperature for 10 minutes, then transfer to 4 ℃ and store in the dark for 24 hours, the final volume of the reaction solution is 1 ml; prepare 2 μM, 1 μM, 0.5 μM DNA-AgNCs aqueous solution, use Excited by 600 nm red light, the measured fluorescence intensity at 660 nm was 80, 35, 18, respectively.

[0024] The compound that obtains with embodiment 1 is test compound

[0025] Select a concentration of 0.5 μM DNA-AgNCs (λex=600 nm, λem=660 nm) aqueous solution, and use Fe(NO 3 ) 3 Titrate with Fe(NO 3 ) 3 As the concent...

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Abstract

The invention belongs to the field of metal ion detection and nucleic acid reagent preparation and in particular relates to a fluorescent nanosilver nucleinate cluster as well as a preparation method and application thereof. A novel fluorescent DNA nanosilver cluster is synthesized by taking micromolecular nucleic acid as a template; the nanosilver cluster is 2-4nm in size; a water solution of the nanosilver cluster is light yellow, the nanosilver cluster is excited by red light of 600nm, relatively strong fluorescent light is emitted at the position of 660nm, the fluorescence quantum yield is 0.2 (compared with bipyridine ruthenium), fluorescent light at the position of 660nm can be quenched by trivalent and divalent iron ions, fluorescent light of DNA-AgNCs has remarkable different fluorescent inductions on trivalent and divalent iron ions, and the sensitivity on divalent iron is much higher than the sensitivity on trivalent iron, so that the total amount of trivalent and divalent iron can be qualitatively and quantitatively analyzed and low-concentration divalent iron (10<-9> to 10<-5> mu M) and trivalent iron (10<-6> to 10<-1> mu M) can be qualitatively analyzed.

Description

technical field [0001] The invention belongs to the field of metal ion detection and preparation of nucleic acid reagents, in particular to a fluorescent nano-nucleic acid-silver cluster and its preparation method and its application in rapidly detecting the content of divalent and ferric iron in a solution by fluorescence spectroscopy. Background technique [0002] In recent years, the use of artificially synthesized probes to selectively sense and identify important ions in the environment and in biological systems, especially heavy metal ions, has aroused great interest; iron ions are widely used in industrial production, and it is also a human body. One of the essential trace elements, iron participates in the transportation and storage of oxygen, and can also promote development; increase resistance to diseases; regulate tissue respiration, prevent fatigue; form hemoglobin, prevent and treat anemia caused by iron deficiency; The skin recovers good blood color; although ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C09K11/06G01N21/64
Inventor 陈秋云陈甜甜
Owner JIANGSU UNIV
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