28 results about "Hemoglobin" patented technology

The invention relates to the technical field of salty essence, in particular to high-temperature cooking resistant salty essence which is characterized by comprising the following components in parts by mass: 30-50 parts of hemoglobin, 10-20 parts of compound amino acid, 5-15 parts of modified polysaccharide, 2-8 parts of a natural antioxidant, 1-5 parts of a flavor enhancer, 0.5-3 parts of a pH regulator and 80-120 parts of deionized water. Hemoglobin is derived from one or more of fresh pig blood, cattle blood, sheep blood, chicken blood, duck blood or goose blood and is obtained after anticoagulation, centrifugation, washing and freeze drying, the purity is larger than or equal to 90%, and the water content is smaller than or equal to 5%. The flavor retention rate of the salty essence can reach 85% or above in a high-temperature cooking environment of 100-135 DEG C, which is far higher than that of the traditional salty essence (40-60%), and the problem that the flavor of the existing essence is easy to lose in high-temperature processing of meat products is effectively solved.

The invention relates to the technical field of medicines, in particular to application of huperzine A in treatment of beta-thalassemia. It is found for the first time that the anti-Alzheimer disease drug huperzine A can be directly combined with gamma-globin to inhibit degradation of gamma-globin through a ubiquitin-proteasome pathway, so that the stability of gamma-globin and the fetal hemoglobin level are improved; the abnormal erythropoiesis and hemolysis phenotype of the beta-thalassemia are improved under the condition of not changing the mRNA expression. Animal experiments show that huperzine A obviously improves hematocrit and the number of red blood cells, reduces the size distribution width of the red blood cells and the proportion of reticulocytes, relieves oxidative stress, hemolysis, splenomegaly and iron overload, and has no obvious liver and kidney toxicity. The invention creatively provides a new strategy for improving HbF by regulating and controlling protein homeostasis instead of transcriptional up-regulation, and provides a new drug action mechanism and application direction for treatment of beta-thalassemia and other hemoglobin diseases.

Disclosed is the use of a composition comprising a TET pathway inhibitor and a ferroptosis inducer in the manufacture of a medicament for the treatment of malignant tumors. It is found that TET1 up-regulates expression of a ferroptosis key regulation gene GCH1 through an NF [kappa] B signal, promotes synthesis of BH4, activates a GPX4 non-dependent pathway, and plays a key hub role in controlling ferroptosis sensitivity. Through combined use of TET pathway inhibitors (including a TET inhibitor and a BH4 synthesis inhibitor), the sensitivity of tumor cells to the ferroptosis inducer can be significantly improved, and the in-vivo use dosage of the ferroptosis inducer is reduced to 1% (1 mg / kg) of the conventional treatment dosage. The composition of the TET pathway inhibitor and the ferroptosis inducer, provided by the invention, can be used for remarkably improving the ferroptosis induction curative effect of various malignant tumors, particularly ferroptosis insensitive tumors, and enhancing the safety and universality of ferroptosis induction treatment.

The application belongs to the technical field of microorganisms, and provides a Streptococcus salivarius subsp. thermophilus HX-ST36, which has a preservation number of CGMCC NO. 32955. The Streptococcus salivarius subsp. thermophilus provided by the application can ferment Astragalus membranaceus Bunge-Angelica sinensis. In a cold coagulation and blood stasis type primary dysmenorrhea model mouse, the fermentation product thereof can significantly inhibit the writhing response, and can realize the effects of relieving dysmenorrhea and tonifying blood by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissue and increasing the hemoglobin (HGB) content. The effect of the fermentation product in removing dampness is verified by improving the dampness signs of a crowd. The application further provides a probiotic preparation and a preparation method of a probiotic fermentation product. The preparation method of the probiotic preparation and the probiotic fermentation product is simple and can be produced on a large scale.

This invention discloses a hematopoietic nutritional steamed bun for piglets, comprising: 35-45 parts corn, 10-15 parts whey powder, 8-10 parts puffed soybeans, 8-10 parts fermented Chinese herbal medicines, 6-8 parts soybean meal, 4-6 parts fish meal, 2-5 parts milk powder, 0.3-0.6 parts compound vitamins, 0.1-0.3 parts compound microminerals, 0.1-0.3 parts zinc oxide, and 0.02-0.05 parts 5-hydroxytryptophan; the fermented Chinese herbal medicines include: 8-12 parts Codonopsis pilosula, 8-12 parts Astragalus membranaceus, 6-10 parts Aucklandia lappa, 8-12 parts Atractylodes macrocephala, 8-12 parts Poria cocos, 12-17 parts longan pulp, 12-17 parts Rehmannia glutinosa, 8-12 parts dried ginger, and 12-17 parts prepared licorice root; the fermented Chinese herbal medicines are obtained by fermentation after ultra-fine grinding of the herbal raw materials. This invention uses fermented Chinese herbal medicines and further optimizes the strains and fermentation process. By scientifically combining various functional nutrients, it systematically promotes hemoglobin synthesis, regulates the intestines, and enhances immune function in piglets, targeting the physiological characteristics of piglets during the peripartum and weaning transition periods, thereby achieving the comprehensive effect of promoting growth and reducing mortality.

The application provides a glycated hemoglobin dissociation reagent and application thereof, and belongs to the technical field of biological detection. The dissociation reagent comprises 10-50 mM Tris buffer, 0.2-0.5 M ammonium chloride, 0.5%-1.0% (v / v) triton X-100, 0.2 wt%-1.0 wt% BSA and 0.02-0.2 M sodium chloride. When the dissociation reagent is used to dissociate a whole blood sample, the hemoglobin can be dissociated by mixing the dissociation reagent with the whole blood sample for 20-60 s, and a clear and transparent solution is obtained. The dissociation reagent can be used to prepare a kit for dissociating or / and detecting glycated hemoglobin. The glycated hemoglobin obtained by using the dissociation reagent can be directly used for fluorescence immunoassay, the interference of the color of hemoglobin itself on immunochromatographic detection is avoided, and the sensitivity is high and the repeatability is good.

Provided is a method for measuring glycated hemoglobin in a sample containing hemoglobin and plasma, the method including reacting glycated hemoglobin in a sample containing hemoglobin and plasma, a proteolytic enzyme, and a fructosyl peptide oxidase to generate hydrogen peroxide, and measuring the generated hydrogen peroxide, wherein the reaction is performed in the presence of ferrocyanide and a ferrocene derivative.

The invention relates to the technical field of deep learning, in particular to an extracorporeal circulation data analysis method and system based on deep learning, and the method comprises the following steps: collecting pump rotating speed, pump flow, arterial perfusion pressure, arteriovenous blood oxygen saturation, hemoglobin and lactic acid, carrying out the time alignment, completing the time sequence coding, and forming state characterization; the method comprises the following steps: correcting a state evolution direction by combining a relationship between oxygen delivery and oxygen extraction, introducing a lactic acid change trend to constrain time sequence consistency, constructing a circulating resistance matching perfusion capability according to flow and pressure, continuously correcting a state change direction, and generating an operation state analysis result. Oxygen delivery and oxygen extraction constraints are introduced to correct state evolution, a lactic acid trend is combined to strengthen time sequence consistency, resistance constraints are formed by perfusion pressure and flow, perfusion capacity changes are continuously corrected, and nonlinear state expression stability and overall interpretation coherence are improved.

Systems, devices, and methods for performing transabdominal fetal oximetry and / or transabdominal fetal pulse oximetry using physiological characteristics and / or calibration factors can receive a physiological characteristic of a pregnant mammal and determine one or more potential effects of the physiological characteristic on the behavior of a light signal projected into the abdomen of the pregnant mammal. Then, in response to the effects, a calibration factor for the light signal can be determined. Then, the calibration factor can be used to calibrate fetal detection electronic signals so that levels of fetal hemoglobin oxygen saturation can be determined.

To provide a device and a method for easily hemolyzing / diluting puncture blood even is the device is an A1c measuring device using the HPLC method.SOLUTION: Glycosylated hemoglobin (A1c%) is measured by a chip set that is formed of a conical or pyramid-shaped chip made of resin that can suck up puncture blood by a capillary phenomenon, a container that can store the chip, and a lid body that can seal the container, and can collect the puncture blood and hemolyze and dilute the blood, the chip set capable of collecting puncture blood and hemolyzing and diluting the blood, wherein the container has an inner diameter larger than the maximum outer diameter of the chip by 0.5 to 5 mm, and the container has therein a flange that has a columnar shape having a height allowing storage of the chip and is at a position 5 to 15 mm from a top face of the container, and a method for collecting puncture blood and hemolyzing / diluting the blood by using the chip.SELECTED DRAWING: Figure 7

This utility model relates to the field of comprehensive utilization technology of livestock and poultry blood, and discloses a device for extracting hemoglobin from livestock and poultry blood. The device includes a reaction vessel with an inlet at the top and an outlet at the bottom. An internal stirring shaft with several shearing blades is installed on the stirring shaft. A hollow jacket for condensation is provided on the outer wall of the reaction vessel. Several ultrasonic generators are detachably mounted on the reaction vessel. Several connecting pipes are connected through the reaction vessel, with a positioning sleeve with a sealing groove at the end of each connecting pipe and a sealing ring fitted on the positioning sleeve. The ultrasonic generators are sealed to the reaction vessel body via an installation kit. This utility model has a simple structure, compact design, and is easy to use. The ultrasonic generators are detachably mounted on the reaction vessel body and sealed by the installation kit, improving the processing efficiency of livestock and poultry blood, ensuring thorough cell disruption, and facilitating hemoglobin extraction. Simultaneously, the hollow jacket on the outside of the reaction vessel for cooling and condensation effectively reduces the temperature rise caused by the operation of the ultrasonic generators.

The utility model discloses a pig blood cell protein powder raw material premixing homogenizer which comprises a high-pressure tank body, the upper end of the high-pressure tank body is fixedly connected with a defoaming agent box, the surface of the defoaming agent box is connected with a round rod in a sliding mode through a through hole, one end of the round rod is fixedly connected with a round block, and the other end of the round rod is fixedly connected with a push plate. And scales are processed on the surface of the defoaming agent box. Through the arrangement of the high-pressure tank body, the defoaming agent box, the round rod, the push plate, the round block and the hose, when a defoaming agent needs to be added into a raw material in the high-pressure tank body, an operator pulls the round block, the round block drives the round rod to move, and the round rod drives the push plate fixed at the other end to move; through the mode, an operator can quickly add a defoaming agent solution in a proper proportion into the high-pressure tank body to be mixed with raw materials, so that the condition that the premixing effect of the pig blood cell protein powder raw materials is poor is avoided, and the working effect of the premixing homogenizer is improved.

This application relates to a cream for promoting pet hair growth and its preparation method, belonging to the technical field of pet biomedicine. It comprises the following raw materials in weight percentages: microencapsulated fish oil 8%-10%; hemoglobin peptide 6%-12%; silkworm pupa protein powder 3%-5%; hydrolyzed chicken liver powder 2%-5%; yeast extract 1%-3%; rosemary extract 0.005%-0.01%; and antioxidant 0.1%-0.4%. The hemoglobin peptide is selected from one or more of MLL peptides and WDL peptides. The amino acid sequence of the MLL peptide is methionine-leucine-leucine; and the amino acid sequence of the WDL peptide is tryptophan-aspartic acid-leucine. This application has synergistic and long-term stable effects, is suitable for dogs, cats, and other pets, is safe with no side effects, and can be mass-produced.

Inorganic anions nitrate and nitrite influence metabolic rate and glucose homeostasis. Infusion of nitrite iv caused an acute drop in resting energy expenditure (oxygen consumption) and nitrate, when given perorally, caused a reduction in oxygen consumption during exercise and a depression of the increase in blood glucose observed after an oral glucose tolerance test. The doses of nitrate and nitrite did not cause any detectable change in methemoglobin levels of blood. Also, nitrate and nitrite did not alter lactate levels in blood. This discovery provides useful treatments to regulate the energy expenditure and glucose homeostasis of a mammal by administration of inorganic nitrite and / or nitrate.

The invention discloses biomimetic catalytic nano-particles, a preparation method thereof and application of the biomimetic catalytic nano-particles in preparation of cerebral hemorrhage treatment drugs, and belongs to the technical field of biomedical nano-particles. The preparation method comprises the following steps: S1, preparing a mixed solution A by taking polybasic carboxylate and monobasic carboxylate as raw materials; s2, uniformly mixing a ceric ammonium nitrate solution, a heme oxygenase-1 solution and a deferoxamine solution to obtain a mixed solution B; s3, mixing and stirring the mixed solution A and the mixed solution B to prepare the biomimetic catalytic nanoparticles. The preparation method is simple and mild in condition, the prepared biomimetic catalytic nano-particles have efficient focus targeting performance, the multi-mechanism synergistic treatment effect of hematoma toxicity (removing heme, iron and ROS) and tissue repair (promoting angiogenesis and neuroprotection) is achieved at the same time, and the biomimetic catalytic nano-particles are remarkable in clinical treatment effect and excellent in biological safety and have good application prospects. Good clinical transformation prospects are realized.

This invention relates to the field of pharmaceutical technology, providing memantine derivatives, their preparation methods, and their application in the preparation of drugs for treating soluble cyclooxygenase-mediated diseases. The memantine derivatives provided by this invention have typical urea or amide structures. The urea and amide structures serve as the primary pharmacophores of sEH (hydrophobic epoxide hemoglobin). The memantine moiety, as a hydrophobic segment, interacts with the receptor hydrophobically. Molecular docking shows that the memantine moiety, as a hydrophobic segment, interacts with the receptor hydrophobically, particularly when both R1 and R2 are methyl groups, which enhances the van der Waals forces. Therefore, the memantine derivatives provided by this invention exhibit high inhibitory activity against human (HsEH) and murine sEH (MsEH), and can be used as sEH inhibitors in the preparation of drugs for treating soluble cyclooxygenase-mediated diseases, showing broad application prospects.

This invention relates to the field of traditional Chinese medicine preparations, and discloses a topical preparation of black alum for treating malaria and rapidly regulating anemia. It is composed of black alum, cinnabar, artemisia annua extract, dichroa febrifuga extract, angelica sinensis extract, astragalus membranaceus extract, and pharmaceutical excipients. Based on black alum and cinnabar, this invention incorporates artemisia annua extract and dichroa febrifuga extract. Black alum exerts its insecticidal effect by disrupting the cell membrane structure of Plasmodium; cinnabar inhibits nucleic acid synthesis in Plasmodium; artemisinin interferes with mitochondrial function in Plasmodium; and dichroa febrifuga alkaloids inhibit protein synthesis in Plasmodium. The preparation is made into a nasal powder, administered through nasal inhalation. It is directly absorbed into the bloodstream through the rich capillaries of the nasal mucosa, rapidly replenishing the body's iron and promoting hemoglobin synthesis. The added angelica sinensis and astragalus membranaceus extracts have blood-tonifying and qi-boosting effects, enhancing the body's hematopoietic function, improving microcirculation, and accelerating the improvement of anemia symptoms.

To provide: methods of increasing levels of fetal hemoglobin (HbF) in cells; and methods for treating patients suffering from blood cell diseases, including those associated with reduced amounts of functional adult hemoglobin (HbA), such as sickle cell disease and β-thalassemia.SOLUTION: A method for increasing expression of a fetal hemoglobin (HbF) in a eukaryotic cell comprises contacting the cell with an inhibitor of a target protein or target protein complex that serves to regulate HbF expression. Optionally, the target protein is Cullin 3 (CUL3) or Speckle-type POZ protein (SPOP).SELECTED DRAWING: Figure 1

The invention concerns the field of process engineering and relates to a method for in-ovo sex determination in an unopened avian egg, the method for example being used in commercial chicken breeding. The problem addressed by the invention is that of specifying a method by means of which in-ovo sex determination in an avian egg is made possible with a high prediction accuracy. The problem is solved by a method in which the following method steps are carried out: providing a fertilized avian egg incubated for a maximum of 9 days, detecting the position of the blood-carrying cardiovascular system in the avian egg, irradiating the avian egg, acquiring and storing absorption spectra using at least one detector and storage device, detecting and selecting the blood-specific absorption spectra and the non-blood absorption spectra and subsequently compensating the absorption spectra, performing an evaluation by determining at least one value of the oxygenation of the haemoglobin, classifying the blood-specific absorption spectra, determining the sex of the embryo.

The application provides an abdominal cavity drainage liquid hemoglobin real-time monitoring device and a monitoring method. The monitoring device comprises a sensing bottle, a control box and a temperature sensor. The sensing bottle is connected with the control box through an outer wall surface. The temperature sensor is connected with the control box. The control box outputs 100KHz and 500KHz sine wave voltage signals to a pair of electrodes. The two voltage signals appear alternately. The application solves three defects of traditional methods, i.e., unclear, inaccurate and slow reaction, and provides a standardized and quantitative early warning scheme for postoperative hemorrhage.

The invention relates to the field of process engineering and concerns a method for in-ovo sex determination on the unopened bird egg, which is used, for example, in commercial chicken farming. The object of the invention is to provide a method by which the in-ovo sex determination of the bird egg is made possible with a high degree of predictive accuracy. The task is solved by a procedure in which the following procedural steps are carried out: - Providing a fertilized bird egg that has been incubated for a maximum of 9 days, - Determining the location of the blood-conducting cardiovascular system in the bird egg, - Irradiating the bird's egg, - Acquisition and storage of absorption spectra with at least one detector and storage device, - Detecting and selecting the blood-specific absorption spectra and the absorption spectra of foreign substances and subsequently compensating the absorption spectra, - Evaluate by determining at least one value of hemoglobin oxygenation, - Classifying blood-specific absorption spectra, - Determining the sex of the embryo.

The application discloses a kind of tissue sample decoloring reagent and the method for processing tissue, and the component of decoloring reagent includes hydrogen peroxide, anhydrous ethanol, polyethylene glycol octylphenyl ether and ethylenediaminetetraacetic acid.The method for processing tissue described in the application includes the tissue sample to be processed is immersed in decoloring reagent processing.Compared with prior art, the application uses hydrogen peroxide as the main decoloring component, removes endogenous autofluorescence such as hemoglobin, lipofuscin etc. in the interior of animal tissue by using its strong oxidizing property, and simultaneously removes residual pigment by using ethylenediaminetetraacetic acid and anhydrous ethanol for auxiliary decoloring, polyethylene glycol octylphenyl ether improves tissue permeability and enhances molecular penetration capacity.The application is suitable for various animal tissue samples, can prepare animal tissue sample thick section in a short time, and under the condition that various staining techniques are compatible and the structure of preserved tissue is complete, endogenous autofluorescence in tissue is removed to the greatest extent.

This invention discloses a tumor adjuvant nutritional preparation based on *Pterocarya stenoptera* oil, its preparation method, and its application. The active ingredients of this preparation are composed of *Pterocarya stenoptera* oil, ω-3 fatty acids, and arginine in a mass ratio of (1-3):(1-2):(1-2), preferably 2:1:1. The preparation method includes mixing the above components evenly, followed by high-pressure homogenization and aseptic emulsification to produce an oral liquid, enteral nutrition emulsion, or powder. This preparation can effectively improve chemotherapy-induced bone marrow suppression, significantly increase white blood cell, red blood cell, and hemoglobin levels, enhance serum superoxide dismutase activity, reduce oxidative stress damage, and significantly alleviate gastrointestinal adverse reactions such as nausea, vomiting, and loss of appetite in cancer patients undergoing radiotherapy and chemotherapy, improving patients' nutritional status and quality of life, and exhibiting good safety.

Provided are a sample analysis method comprising: separating hemoglobin in a sample from the sample comprising the hemoglobin, in an alkaline solution by capillary electrophoresis, wherein the alkaline solution comprises a cationic polymer having a secondary amino group and a quaternary ammonium base.

This invention discloses a plant growth regulator for controlling nitrogen fixation and stress resistance in plants and its application, belonging to the field of agricultural biotechnology. The plant growth regulator uses molybdenum disulfide nanosheets as the active component, which are dispersed in an aqueous solution to prepare a spray solution. A surfactant is added to enhance leaf wettability, and the solution is applied to soybean plants during their growth period via foliar spraying. The molybdenum disulfide nanosheets are internalized into mesophyll cells through open stomata in the leaves, and further undergo biotransformation to form inorganic molybdenum (Mo(VI)) and organic molybdenum (Mo-RCOOH), which are then transported to the roots and root nodules. By delaying root nodule senescence, upregulating the expression of the soybean hemoglobin gene, and prolonging the active nitrogen fixation period, the symbiotic nitrogen fixation efficiency of soybeans is significantly improved. Under adverse conditions such as nitrogen limitation and salt stress, this physiological process significantly improves the overall growth status of soybean plants.