404 results about "Melanin" patented technology

The invention relates to the technical field of genetic engineering, and particularly discloses a genetic engineering bacterium for producing melanin as well as a construction method and application of the genetic engineering bacterium. According to the application, the yield of the melanin can be effectively increased by modifying a metabolic pathway of the genetically engineered bacterium, and the yield of the melanin reaches 6.2 g / L and 18.5 g / L respectively through shake-flask culture and 5L fermentation tank culture. Compared with the prior art, the yield of melanin produced by the genetically engineered bacterium is improved by 30% at the shake flask fermentation level, the fermentation yield of a fermentation tank is improved by 4.3 times, in addition, expensive tyrosine is changed into glucose as a fermentation precursor substance, the production cost is greatly reduced, and an efficient and feasible solution is provided for large-scale production of melanin.

The invention belongs to the technical field of biological dyes, and discloses a bio-based melanin precursor compound, bio-based melanin as well as a preparation method and application of the bio-based melanin precursor compound and the bio-based melanin. The invention provides a bio-based melanin precursor compound 4-[5-(2, 2-diaminoethyl)-2, 3-dihydroxyphenyl]-1H-indole-5, 6-diphenol, and a preparation method of the bio-based melanin precursor compound. The bio-based melanin precursor compound is stable in chemical property and easy to store. And the catalyst can be used as a raw material to efficiently synthesize bio-based melanin through a catalytic oxidation reaction. The process for preparing the bio-based melanin is simple, mild, high in yield and suitable for large-scale industrial production. The bio-based melanin prepared by the invention has good application performance during dyeing, does not generate harmful substances, and is safe and environment-friendly.

The invention relates to the technical field of scalp care, in particular to a hair-blacking composition for promoting melanin secretion and a preparation method of the hair-blacking composition. Comprising the following components in parts by weight: water, ethanol, a ginseng root extract, a polygonum multiflorum extract, a cacumen biotae extract, a licorice root extract, an eclipta prostrata extract, a ganoderma atrum extract, a rhizoma bletillae root extract, a mulberry leaf extract, radix sophorae flavescentis powder, a glossy privet fruit extract, a raspberry fruit extract and a black sesame extract. According to the technical scheme, the ganoderma atrum extract is prepared through enzymolysis-ultrasonic combined modification, a cell wall structure is decomposed through compound enzyme, then cell remains are further broken through the ultrasonic cavitation effect, and the release rate of active peptide and polysaccharide is increased; the hair-blacking composition can promote the generation of hair melanin so as to blacken hair, improve the hair quality, enable the hair to become tough and elastic, and enable the hair to gradually become black, smooth and firm after being smeared on the scalp for a long time.

The invention discloses an intense pulsed light treatment parameter intelligent regulation and control system fused with spectrum sensing skin monitoring, and relates to the technical field of intense pulsed light treatment parameter regulation and control, and the intense pulsed light treatment parameter intelligent regulation and control system comprises the following steps: collecting the melanin content of skin in an intense pulsed light treatment area by using a high-resolution spectrum sensor, and obtaining melanin distribution data of the treatment area; carrying out data preprocessing, and carrying out standard melanin content analysis to obtain the standard melanin content of the treatment area; predicting the change of the reference melanin content of the treatment area to obtain reference content prediction data; the energy density of the intense pulsed light treatment is regulated and controlled; the method is used for solving the problems that when an existing strong pulsed light treatment parameter regulation and control technology is used for regulating and controlling the energy density of strong pulsed light treatment, the energy density of the strong pulsed light treatment cannot be automatically and accurately regulated and controlled according to the specific melanin content of an irradiated user skin area; and regulation and control in advance are carried out according to the change of the melanin content.

The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.

The invention discloses a melanin-producing aureobasidium for co-producing pullulan and converting American ginseng and application, and relates to the technical field of bioengineering fermentation. The melanin producing aureobasidium is aureobasidium melanogenum F8449, and the preservation number of the aureobasidium melanogenum F8449 is CGMCC (China General Microbiological Culture Collection Center) No. 23228. The strain can be used for simultaneously producing pullulan and biologically converting American ginseng extract in liquid, so that efficient utilization of fermentation substances is realized, the fermentation cost is reduced, and the fermentation income is increased.

The invention relates to a composition for fading acne marks and application thereof. The composition for fading acne marks comprises PDRN, a lactobacillus / algae extract fermentation product and a fig fruit extract. The acne mark fading composition disclosed by the invention has a synergistic interaction effect, and can efficiently inhibit release of inflammatory factors and fade red marks caused by vasodilatation from the source; the traditional Chinese medicine composition has the advantages that melanin generation can be more synchronously intervened, black marks caused by pigmentation can be effectively prevented and improved, red acne marks and black acne marks can be simultaneously improved through multiple channels by the synergistic mechanism, and the traditional Chinese medicine composition is mild and non-irritant and is more suitable for improving mixed acne marks.

The application discloses application of a complex containing Dendrobium officinale leaf extract in preparation of a whitening and freckle-removing preparation, and the complex comprises the Dendrobium officinale leaf extract, a Rhizoma Polygonati extract and beta arbutin. After the components of the complex are mixed in a specific ratio and fully combined and synergized, the ability of inhibiting tyrosinase activity can be significantly improved by 300%, 175% and 87% respectively compared with the Dendrobium officinale leaf, the Rhizoma Polygonati and the beta arbutin, the melanin generation in zebra fish within 72 hours can be effectively inhibited, the melanin content can be obviously reduced, the melanin inhibition rate is increased by 394%, 532%, 24.14% and 37.39% respectively compared with the Dendrobium officinale leaf group, the Rhizoma Polygonati group, the beta arbutin group and the Dendrobium officinale leaf-Rhizoma Polygonati group, the Dendrobium officinale leaf-Rhizoma Polygonati complex can obviously improve the melanin inhibition rate in zebra fish, the combination of the two and the beta arbutin can make the melanin inhibition rate reach 98.70%, and the whitening and freckle-removing effect of skin can be greatly enhanced.

The invention provides a mobile terminal skin parameter inversion method based on spectral information, and the method comprises the following steps: constructing an optical model according to the characteristics of a skin layered structure and a spectral response mechanism of three skin parameters: melanin content, hemoglobin concentration and dermis thickness, and building a skin parameter and reflection spectrum database based on the model; based on the database, establishing a skin multi-parameter inversion MLP neural network structure adaptive to the collection characteristics of a mobile phone end sensor, and screening an optimal four-waveband channel through multi-waveband combination traversal; the mobile phone spectrum sensor is subjected to wave band optimization, data acquisition and skin parameter inversion. According to the method, through efficient decoupling of a nonlinear coupling relation among multiple skin parameters by waveband optimization and an MLP model, and targeted design of four optimal visible light narrow-band channels, synchronous quantitative detection of melanin, hemoglobin and dermis thickness is realized, core physiological parameters from epidermis to dermis superficial layer are covered, information coverage is comprehensive, and specificity is high.

The invention discloses a whitening antioxidant composition containing a citrus chachiensis hortorum extract. The whitening antioxidant composition is prepared from the citrus chachiensis hortorum extract, ergothioneine and PDRN. The weight ratio of the citrus chachiensis hortorum extract to the ergothioneine to the PDRN is (0.1 to 5): (0.001 to 0.1): (0.01 to 1). Through ternary compounding of the citrus chachiensis hortorum extract, the ergothioneine and the PDRN, the synergistic effect of'inhibiting melanogenesis, removing ROS (reactive oxygen species) and improving antioxidant enzyme activity 'is realized, the expression of melanin precursor protein Pmel17 is inhibited, the levels of NNT and GSH-Px1 are improved, the content of reactive oxygen species (ROS) is reduced, and the problem of single effect in the prior art is solved. The composition has excellent whitening and anti-oxidation effects, is high in stability, good in safety and wide in application range, and can be widely applied to functional cosmetics such as whitening essence, face cream, emulsion and sunscreen cream.

Existing pulse oximeters have impaired accuracy in measuring blood oxygen levels (SpO2) in patients of color, where the presence of melanin in the skin tends to interfere with measurements of pulse oximetry, resulting in an overestimation of SpO2. Thus, the pulse oximeter often reports a higher oxygen saturation for patients with darker skin tones, often with increasing bias as the actual saturation (SaO2) decreases. This bias is further reinforced by calibration based on individuals with light skin pigmentation, which may lead to inequitable healthcare for patients of color. Our proposed solution is to modify current pulse oximetry calculations—which utilize the relative tissue absorbance of red and infrared light to estimate SpO2—to account for the concentration of melanin by additionally measuring the skin absorbance of UV˜A light. This derived concentration of melanin can then be used to modify the pulse oximetry algorithm output, thus estimating SpO2 more accurately for patients of color.

The invention discloses application of Bombyx mori neuropeptide BommoOKAtype4 in preparation of a medicine for inhibiting melanoma cell growth, and belongs to the technical field of medical biology. Aiming at the technical problems that melanoma is high in malignancy degree, easy to transfer and limited in treatment means, and whether the bombyx mori neuropeptide BommoOKAtype4 can directly inhibit the growth of melanoma cells is not clear yet when the bombyx mori neuropeptide BommoOKAtype4 is mainly used for skin whitening before, the invention provides the application of the bombyx mori neuropeptide in preparing the medicine for inhibiting the melanoma cells. According to the silkworm neuropeptide, gene expression and protein functions of melanin synthesis key enzymes TYR, TRP1 and TRP2 are comprehensively inhibited by down-regulating expression of a core transcription factor MITF, so that melanoma cell proliferation is directly inhibited while melanin synthesis is inhibited, and therefore, the silkworm neuropeptide can be used for preparing drugs for treating melanoma and has a wide application prospect. A novel peptide candidate substance is provided for treatment of melanoma.

This invention relates to the field of edible fungi technology, and discloses a new strain of Auricularia heimuer and its applications. The Auricularia heimuer strain JH-Y6 of this invention was deposited at the China Center for Type Culture Collection (CCTCC) on November 20, 2023, with accession number CCTCC No: M 20232280. Auricularia heimuer JH-Y6 possesses high tyrosinase activity, high melanin production, and excellent agronomic traits, and can be used in the screening and breeding of Auricularia heimu, the extraction process of melanin from Auricularia heimu, and its cultivation. It can also be used in the deep processing of food or health products.

The invention relates to a gene expression cassette and application thereof. The gene expression cassette comprises a tyrosinase gene, a transcriptional repressor protein gene, a melanin response promoter and a reporter gene, and the reporter gene is located at the downstream of the melanin response promoter and is regulated by the promoter. The gene expression cassette with a specific structure is designed, melanin in cells can be responded, a melanin biosensor can be further constructed by utilizing the gene expression cassette, the gene expression cassette has good specificity, and high-throughput screening of strains can be realized. Furthermore, ALE evolution is carried out on the strain for producing melanin, high-throughput screening is carried out based on a designed sensor, the strain for producing melanin with high yield is obtained, through shake-flask culture and 5L fermentation tank culture, the yield of melanin reaches up to 10.84 g / L and 29.04 g / L respectively, and an efficient and feasible solution is provided for large-scale production of melanin.

The present specification relates to a composition including a new amide-based compound derived from an amino acid structure such as valine, leucine, phenylalanine, proline, pipecolic acid, or the like, wherein the composition exhibits a skin anti-aging or skin reverse-aging effect by restoring the size and number of dendrites of aged melanin-producing cells to those of young cells. As a result, the composition may exhibit a skin brightening effect by inhibiting the amount of melanin produced in the melanin-producing cells.

The invention discloses a compound with tyrosinase inhibitory activity and application of the compound in preparation of whitening products, and belongs to the technical field of medicinal chemistry. The compound is 5-(4-hydroxyphenyl)-3-O-glucosyl valeric acid, and is a novel compound. The compound can significantly inhibit the activity of tyrosinase, has the ability of blocking tyrosinase from catalyzing tyrosine to be converted into melanin precursor substances, and directly inhibits the generation of melanin, thereby showing better application prospects and values in skin whitening or skin diseases.

The application discloses a kind of sulphur-containing polydopamine melanin nanoparticles and preparation method and application, preparation raw materials include polyhydroxy compound and sulphur-containing compound, the weight ratio of the polyhydroxy compound and the sulphur-containing compound is (1-100) :(1-100), the preparation raw materials also include water, organic solvent and acid-base regulator.The application occurs self-polymerization reaction under alkaline condition with dopamine and sulphur-containing compound, without additional crosslinking agent and oxidant, and the nanoparticles obtained have excellent photothermal performance.In photothermal therapy, biomedical imaging, water purification, functional coating and other aspects, it shows great application potential.And preparation reaction condition is mild, without additional catalyst and oxidant, process is safe, post-processing is simple, and no harmful waste water and waste residue to environment, meet the requirements of green chemistry.

This invention relates to the cosmetic use of a composition for improving gray hair. The composition of this invention promotes melanin synthesis in hair by activating the Wnt / β-catenin signaling pathway, thereby exhibiting significant and excellent effects in inhibiting, preventing, improving, alleviating, delaying, or treating gray hair (white hair syndrome).

The present invention relates to a composition comprising plant extracts. The composition according to the present invention has a skin whitening effect by reducing the total amount of melanin and tyrosinase activity in melanocytes of the skin, promotes skin regeneration and increases skin elasticity or reduces skin wrinkles by promoting collagen synthesis and inhibiting collagenase activity in fibroblasts of the skin, has an anti-inflammatory effect or a skin soothing effect by inhibiting NO generation, increases the amount of moisture in the skin and has a moisturizing effect by promoting generation of hyaluronic acid in fibroblasts, and has an antioxidant effect by scavenging free radicals. In addition, since the composition of the present invention exhibits a broad antibacterial effect against various bacteria, the composition may be used as a cosmetic composition, a pharmaceutical composition, a skin external preparation, or a food composition.

The invention provides a soft capsule helpful for improving the function of chloasma as well as a preparation method and application thereof, and relates to the technical field of health care products. The soft capsule comprises a mulberry leaf extract, a seville orange flower extract, a grape seed extract, an almond extract, donkey-hide gelatin powder, vitamin C and vitamin E. The soft capsule provided by the invention exerts the overall effect of improving chloasma through a multi-target synergistic mechanism from inside to outside: the mulberry leaf extract and the grape seed extract block melanogenesis from the source and resist ultraviolet damage by inhibiting tyrosinase activity and strongly resisting oxidation; the seville orange flower extract is used for soothing liver and regulating qi, and regulating internal pathogenesis; the almond extract and the donkey-hide gelatin powder have the effects of promoting blood circulation to remove blood stasis and nourishing yin and blood, improve microcirculation and nourish skin; vitamin C and vitamin E form an anti-oxidation cycle, so that melanin intermediates are directly reduced, and cell membranes are protected. All the raw materials supplement each other to jointly achieve the comprehensive effects of fading stains, brightening the skin color and improving the skin quality.

This invention discloses a composition for fading dark spots, whitening, and firming the skin, and its preparation method, specifically belonging to the field of cosmetic technology. The composition contains extracts of *Bretschneidera sinensis* bark, hydroxypinazone 9-cis-retinate, sage lactone, tetrahydromagnoliol, and quercetin dimer. The composition provided by this invention can significantly reduce skin melanin content, improve skin brightness, and significantly improve skin elasticity and firmness. The synergistic effect of the components effectively achieves the effects of fading dark spots, whitening, and firming the skin. The preparation process provided by this invention is stable and controllable, suitable for large-scale production, and provides a novel and highly effective skincare composition and application solution for the cosmetic field.

Lightweight melanin composite sound protection materials that are easy to fabricate into final components, at reasonable cost, yet still offer sound protection properties on par with heavier materials are disclosed.

A whitening composition containing the following components: Mori folium, Panacis quinquefolii radix, (-)-podophyllotoxin, and (+)-glaucarubinone. Further provided is the use of the whitening composition in the preparation of a product for whitening the skin, or a product for inhibiting melanin synthesis, or a product for improving skin pigmentation.

The present invention provides a method for producing melanin and / or polyhydroxyalkanoate (PHA) materials and / or proteins from microorganisms by means of C1-C1 and / or C2-C2 resources and uses thereof. The Halomonas is modified, so that the Halomonas can be used for producing melanin, and the co-production of extracellular melanin and intracellular PHA (Polyhydroxyalkanoate) and / or protein is realized. According to the method, a platform for sustainable and high-benefit production of melanin, PHA and / or protein by using carbon dioxide is established, and the method plays an important role in further reduction of production cost and improvement of sustainable development.

The application discloses a kind of multi-effect repair type collagen and its application in the technical field of synthetic biology, the application selects transdermal peptide TD-1, the coding gene of the transmembrane region of human XVII type collagen, extracellular sixteenth non-collagen region and extracellular fifteenth collagen region and His tag is connected in series, and gene sequence is optimized by pichia pastoris codon selection preference, then obtains T-COL17R3 by construction and expression. Through efficacy experiment, it is verified that T-COL17R3 compared with similar products on sale, not only excellent transdermal performance, also has more optimal ability of promoting cell proliferation, migration, anti early glycation product ketone amine and anti late glycation product dicarbonyl compound, better free radical scavenging ability and anti-elastase ability, and can reduce the synthesis of melanin in B16-F10 cell, therefore, T-COL17R3 has excellent application potential in the development of drug composition or skin care product with repair, anti-wrinkle firming or whitening effect.

The present invention provides radiolabeled 2,3,4,5-tetrahydro-1H-[1,4]diazepino[1,7-α]indol-9-yl]pyridin-2(1H)-one derivatives of general formula (I) or salts thereof, as well as precursors of the radioligands of general formulae (II) and (III), processes for their preparation, and intermediates of the processes. The radiolabeled compounds enable visualization and quantification of melanin-concentrating hormone receptor 1 (MCHR1) in various target tissues, including monitoring MCHR1 in such target tissues. The compounds exhibit high binding affinity for MCHR1 and are able to cross the blood-brain barrier, thus facilitating diagnosis of various disease states in human clinical studies.

The invention relates to a method for preparing a structural color pattern by using a melanin photon material, and belongs to the technical field of structural colors. The method comprises the following steps: S1, under an alkaline condition, carrying out a reaction on PS microspheres and dopamine hydrochloride in a tris (hydroxymethyl) aminomethane-hydrochloric acid buffer solution, and centrifuging to obtain PS (at) PDA microspheres; s2, the PS (at) PDA microspheres are prepared into PS (at) PDA microsphere dispersion liquid, the PS (at) PDA microsphere dispersion liquid is dropwise added to the surface of a silica gel plate, and PS (at) PDA structural color pellets are obtained through drying treatment; and S3, the PS-coated PDA structural color pellets are prepared into PS-coated PDA structural color pellet dispersion liquid, atomization deposition is conducted through a mask technology, and a structural color pattern is obtained. By regulating and controlling the particle size of the PS microspheres and the thickness of the PDA shell layer, the structural color material with uniform color and high color visibility and saturation can be prepared, and finally the preparation of the full-chromatographic structural color covering the whole visible spectrum area is realized.

The present invention relates to a cosmetic composition for skin elasticity enhancement, skin wrinkle alleviation, and skin whitening, comprising a fermented extract of Perilla frutescens var. acuta Kudo as an active ingredient. The cosmetic composition comprising a fermented extract of Perilla frutescens var. acuta Kudo as an active ingredient, according to one embodiment of the present invention, comprises components derived from natural products and thus is harmless to the human body and exhibits remarkable efficacy in increasing collagen production and ability to inhibit melanin production, thereby being useful for skin elasticity enhancement, skin wrinkle alleviation, and skin whitening.

The invention belongs to the technical field of disease and pest control, discloses application of tyrosine hydroxylase as a target spot in prodenia litura pest control for the first time, screens out a dopamine synthesis inhibitor AMPT aiming at tyrosine hydroxylase, and develops a technology for efficiently controlling prodenia litura. According to the technology, AMPT is injected into the fourth-instar larvae of the prodenia litura, and the melanin deposition of the exoskeleton of the larvae is reduced, the color of the body surface becomes light and the athletic ability is remarkably reduced by utilizing the dopamine synthesis inhibition function of AMPT. On one hand, reduction of melanin deposition weakens the environmental adaptability of pests, and the exposure risk of the pests in the natural environment is increased; on the other hand, the migration behavior of the pests is directly influenced by the decline of the exercise ability, and the population diffusion and hazard range of the pests are limited. The dual-action mechanism reduces the environmental adaptability and survival competitiveness of the pests, and provides a new strategy for green prevention and control.

The invention discloses jellyfish polypeptide with tyrosinase inhibitory activity and application of the jellyfish polypeptide, and belongs to the technical field of biology. A jellyfish crude polypeptide is prepared, a jellyfish polypeptide GEPINPEAWLWYYKYVG is identified and optimized from the jellyfish crude polypeptide, and experiments prove that the jellyfish polypeptide provided by the invention can inhibit the activity of tyrosinase in vitro, can inhibit melanin secretion of B16-F10 mouse melanoma cells, and can inhibit the activity of tyrosinase in the cells. Therefore, the jellyfish polypeptide can be used as a tyrosinase inhibitor and an anti-melanin synthesis agent and is applied to the fields of cosmetics, medical treatment and the like. Experiments prove that the jellyfish polypeptide provided by the invention has no cytotoxicity, is derived from ocean, and has no disease risks and religious barriers of terrestrial animals.