271 results about "Tyrosinase inhibitor" patented technology

Disclosed are stable compositions which are effective as skin whitening agents, comprising alpha, beta or gamma substituted mono-, di or polyhydroxy or polycarboxy carboxylic acids; mono-, di, tri or polyhydroxy alkyl compound; and effective amounts of an inhibitor of tyrosinase and a pigmentation reversing reagent. Also disclosed are methods of forming stable compositions of the type described above.

Disclosed are peptides that inhibit the enzymatic activity of tyrosinase, as well as formulations and methods for their use in the reduction of skin pigmentation, and methods of administering the inhibitory peptides in a topical formulation. Peptide sequences disclosed include KFEKKFEK and YRSRKYSSWY.

The present invention is directed to inhibitors of tyrosinase, pharmaceutical compositions comprising such tyrosinase inhibitors, and methods of making and using the same. Specifically, included in the present invention are compositions of matter comprised of at least one 2,4-dihydroxybenzene analog, which inhibit the activity of tyrosinase and which inhibit the overproduction of melanin.

The tyrosinase inhibiting agent of this invention contains a solvent extract from at least one of Gardenia, Sophora and Rosa. When all three are present, it is preferred that the Gardenia extract be in the range of 15-30%, Sophora extract be in the range of 15-30%, and Rosa extract be in the range of 40-65%.

The invention provides a macadamia nut green peel extract, namely macadamia nut green peel polyphenol or macadamia nut green peel aqueous extract which is prepared with the following method: taking macadamia nut green peel powder, adding warm water for water extraction three times, then adding 5% sulfuric acid, keeping quenching for 24h, and filtrating; washing a filter cake with deionized water to remove residual sulfuric acid until the washing liquid is neutral, and obtaining the macadamia nut green peel polyphenol; or washing macadamia nut green peel original crude drug coarse powder with water, putting into boiling water, boiling for 30min, stopping heating, keeping the water temperature at 60 DEGC-70 DEG C, performing ultrasonic extraction for 1h, centrifuging an aqueous extraction liquid at 2000r/min for 4min to obtain a supernatant liquid, then concentrating the supernatant liquid to thick paste, and obtaining the macadamia nut green peel aqueous extract. The macadamia nut green peel extract is used for preparing toning lotion and toning cream. The invention also provides application of the macadamia nut green peel polyphenol or macadamia nut green peel aqueous extract to preparation of cosmetics, as well as application to preparation of a tyrosinase inhibitor and preparation of an antioxidant.

The invention discloses an active polypeptide and tyrosinase inhibitor composition for whitening and freckle removal. The composition comprises polypeptides with a melanogenesis inhibition function and/or polypeptides with a glycosylation resistance function, and other components with a tyrosinase activity inhibition function. The composition is capable of inhibiting melanogenesis, resisting glycosylation, eliminating saccharified dark yellow proteins and eliminating in-vivo free radicals, so as to achieve the aims of improving the skin types, whitening and removing freckles.

The invention discloses application of an aquilaria sinensis peel extract in preparation of whitening cosmetics. The aquilaria sinensis peel extract is prepared by a method comprising the following steps of: by taking aquilaria sinensis peel as the raw material, extracting the aquilaria sinensis peel through ethyl acetate; and drying an extracting solution to obtain the aquilaria sinensis peel extract. The aquilaria sinensis peel extract has an inhibition effect on tyrosinase and can be used for preparing a tyrosinase inhibitor in order to be applied to the whitening cosmetics.

A cosmetic and dermatological composition comprises 2, 3-dihydro-4-hydroxy-2-oxo-1-H-indole-3-acetic acid (4-hydroxy-1-oxindole-3-acetic acid) as an active ingredient for use as a melanin inhibitor and as an anti-tyrosinase enzyme inhibitor. This composition is excellent as a skin whitening agent. Since it also has potent antioxidant and anti-irritant activities it is useful in a cosmetic composition for improving hyperpigmentation disorders of the skin.

The invention relates to a paradimethy-laminobenzaldehyde thiosemicarbazone as tyrosinase restrainer, in particular to the paradimethy-laminobenzaldehyde thiosemicarbazone applied in beauty cosmetics. The invention uses paradimethy-laminobenzaldehyde thiosemicarbazone with high-effect restrain function on the activity of tyrosinase as whitening agent to prepare beauty cosmetics, and provides a relative beauty cosmetics and a preparation. The invention uses paradimethy-laminobenzaldehyde thiosemicarbazone as whitening agent of de-crease beauty cosmetics solution which contains sea fish collagen peptide, paradimethy-laminobenzaldehyde thiosemicarbazone, alcohol, glycerin, methyl glycol, plant essence, conservative and water, that dissolves sea fish collagen peptide into water to obtain solution A, dissolves conservative and paradimethy-laminobenzaldehyde thiosemicarbazone into alcohol to obtain solution B, adds glycerin and methyl glycol into solution B to obtain solution C, mixes the solutions A and C, and adds water to form solution D, and adds plant essence into the solution D.

The invention relates to a tyrosinase polypeptide inhibitor, which consists of a polypeptide. In the polypeptide, one or more amino acids are further subjected to acetylation, amidation, formylation, hydroxylation, lipolyzation, methylation or phosphorylation modification. The tyrosinase inhibitor provided by the invention can be taken as a medical composition, comprises the polypeptide, and further comprises a pharmaceutically-acceptable supporting agent. The medicinal composition provided by the invention can be used for inhibiting the generation of black pigments under the action of activity inhibition of tyrosinase, and can be widely applied to cosmetics such as a whitening agent.

The invention discloses a compound having a tyrosinase inhibitory activity and a synthesis method of the compound, belonging to the fields of foods, cosmetics and medicines. The synthesis method comprises the following steps: carrying out a reaction between 2,4-dihydroxy benzaldehyde and 2,4-dihydroxy acetophenone under the catalysis of boric acid and vitamin C by taking polyethylene glycol 400 (PEG400) as a solvent for 6h so as to achieve the green synthesis of an efficient tyrosinase inhibiting agent; and carrying out gel column chromatography and high performance liquid chromatography separation so as to prepare a pure product of the efficient tyrosinase inhibiting agent. On the basis of mass spectrum and nuclear magnetism analysis, the substance is determined as 1,3,5-tris-(2,4-dihydroxy-phenyl)-pentane-1,5-dione. The compound disclosed by the invention can be used for effectively inhibiting enzymatic browning of fruits and vegetables, the preparation of whitening cosmetics as well as the preparation of a pharmaceutical preparation for preventing and treating human hyperpigmentation diseases and melanoma caused by excess melanin as well as other requirements to inhibit the activity of the tyrosinase.

The invention relates to an application for extracting natural compound (I) from black mulberry, the compound (I) has strong inhibition effect for tyrosinase and is applied for preventing skin mottle, freckle, senile plaque or the like skin chromatosis and whitening, and the compound (I) is a food additive. The activity of the compound (I) is strong proved by the mensuration of the inhibition activity, the compound (I) has good stability and obvious growth inhibition action for the melanoma cell, can reduce the activity of the tyrosinase in the melanoma cell and biological synthesis level of the melanin in the cell.

The invention discloses a tomato extract as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, cleaning a tomato, crushing and homogenizing to obtain a tomato homogenate liquid; mixing the tomato homogenate liquid and water to obtain a mixed liquid; extracting the mixed liquid by ultrasonic assistance with power of 30-40w for 6-10 minutes; extracting in a water-bath kettle at 45 DEG C for 30-40 minutes after the ultrasonic-assisted extraction; centrifuging on a refrigerated centrifuge at 8000r/min and 4 DEG C for 15 minutes after the extraction is completed so as to obtain supernate; filtering the supernate, controlling the pressure of the filtrate to be 0.08-0.1MPa, and concentrating at reduced pressure and 50-65 DEG C; and controlling the temperature of the concentrated residue to be minus 20-minus 40 DEG C, and freezing and drying to obtain the tomato extract. The extract is capable of inhibiting tyrosinase, can be applied to whitening skin-care products as a tyrosinase inhibitor, is safe and reliable in performance, and does not have side effect. The preparation method is simple, and is convenient to operate.

The invention discloses a hyroxy-pyridone derivative with multiple biological activities. The hyroxy-pyridone derivative has any of the following general molecular formulas (please see the specifications), wherein in the general molecular formula 1, R refers to CH2C6H5 or CH3 or CH(CH3)2 or CH(CH3)CH2CH3 or CHCH2(CH3)2 or CH2CH2SCH3, and R' refers to an alkyl radical or a hydrocarbon oxygroup; in the general molecular formula 2, R and R' refer to CH2C6H5 or CH3 or CH(CH3)2 or CH(CH3)CH2CH3 or CHCH2(CH3)2 or CH2CH2SCH3. The invention further discloses a preparation method of the hyroxy-pyridone derivative. The hyroxy-pyridone derivative can be used as a tyrosinase inhibitor or used for retaining freshness of cosmetics or foods.

The invention discloses 3-substituted phenyl propionaldehyde thiosemicarbazone compounds, and a preparation method and application thereof. The structures of the compounds are shown as a formula I, wherein R is any one of 4-F, 4-Cl, 2-Cl, 2,4-Cl2, 4-OCH3, 2-OCH3, 3-OCH3, 4-OC2H5, 4-NO2, 2-NO2, 4-CH3, 4-isopropyl, 3-Br and 2,4-dimethoxy. The preparation method comprises the following step of performing condensation reaction on substituted phenyl propionaldehyde shown as a formula II with thiosemicarbazide in an organic solvent in the presence of an acidic compound to obtain the 3-substituted phenyl propionaldehyde thiosemicarbazone compounds. The research on inhibition activity of heliothis armigera tyrosinase by the compounds of the formula I proves that the compounds have better inhibition effect on the heliothis armigera tyrosinase and can serve as an insect tyrosinase inhibitor for preventing and controlling pests.

The invention relates to a preparation method of Beauveria sp. extract with functiosn of a tyrosinase inhibitor and an antioxidant. The preparation method comprises the following operation steps: 1. selecting and culturing Beauveria sp. Strain; and 2. extracting and refining active components from a fermented product, wherein the refining process comprises primary refining and chromatographic refining. The Beauveria sp. and Beauveria sp. extract provided by the invention have tyrosinase inhibiting and antioxidation active effects, and the refined product has the minimum IC50 (50% inhibiting concentration) of 134.5 mu g/ml to tyrosinase and the minimum IC50 of 87.2 mu g/ml to rat liver cell lipid oxidation; the Beauveria sp. extract can be used as articles for daily use to take into effects of whitening, antioxidation, antiaging, preserving, sterilizing, anti-inflammatory and the like; because the Beauveria sp. extract is produced by microorganism fermentation, the production of the Beauveria sp. extract is not influenced by environment and natural resources, and is easy to industrialize and automatize; the process method has low production cost, simple process, stable process, and high success rate and is easy to adjust and control; and the preparation method is suitable for investment of enterprises in various scales.

The invention relates to a new tyrosinase inhibitor and its usage. The inventive content is that it provides biphenylglycosidicderivatives which can be used in the spheres of medicine and daily-use chemistry as melanin inhibiting agents or brightening agents, speaking in detail, it provides biphenylglycosidicderivatives represented by the above general formula (I), in which R1 stands for hydrogen, hydroxy,oxomethyl or gluconic group, R2 stands for hydrogen, hydroxyl or gluconic group, and R3 and R4 stand for hydrogen or gluconic group; and provides compositions with the said tyrosinase inhibitors. The new tyrosinase inhibitors and the compositions with the new compounds can be used as cosmetics to restrain melanin or brighten, medicines, externally used skin-care agent and so on.

Uses of 2-hydroxy-N-(4-hydroxyphenyl)-benzamide compounds in preparation of tyrosinase inhibitors are disclosed. The uses include aspects of (a) preparing the tyrosinase inhibitors or compositions inhibiting tyrosinase, (b) preparing compositions inhibiting melanogenesis and (c) preparing compositions for preventing or treating pigmentary dermatoses. Test results prove that the 2-hydroxy-N-(4-hydroxyphenyl)-benzamide can inhibit activity of intracellular tyrosinase of B16 cells at the cellular level, thus effectively reducing the amount of melanin synthesized by the B16 cells, and therefore the 2-hydroxy-N-(4-hydroxyphenyl)-benzamide compounds can be used for preventing food brownness degeneration, preparing medicines treating hyperpigmentation skin diseases and preparing whitening cosmetics.

The invention discloses tyrosinase inhibitor microemulsion as well as a preparation method and an application thereof, and belongs to the technical fields of foods, medicines and cosmetics. The microemulsion contains a matrix, a tyrosinase inhibitor norartocarpetin and water, wherein the matrix comprises 40%-60% of surface active agent, 20%-45% of cosurfactant and 5%-20% of oil phase; and the microemulsion is prepared by adding raw components according to a certain ratio. The tyrosinase inhibitor microemulsion can be used for overcoming the defects that the solubility of the natural tyrosinase inhibitor norartocarpetin in water is extremely low. The method provided by the invention is simple to operate and can be used for the spontaneous formation of the microemulsion. Nanoscale microemulsion can be formed by using water or an aqueous solution for diluting the tyrosinase inhibitor microemulsion. The obtained microemulsion can be used for effectively resisting enzymatic browning of fruits and vegetables, and preparing whitening cosmetics and other medicines for preventing and treating human hyperpigmentation diseases and melanomas (caused by excessive melanin) needing to inhibit the activity of tyrosinase.