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Use of black mulberry extract as tyrosinase inhibitor

A technology of tyrosinase and compounds, applied in the field of natural compounds, can solve problems such as lack of preservation methods and economic losses

Inactive Publication Date: 2009-06-10
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, the preservation of fruits, vegetables, fish and shrimps is an urgent problem facing my country's agricultural and aquatic products. Some agricultural and aquatic products, such as fruits, vegetables, fish, shrimps, etc., cannot be treated with timely and effective fresh-keeping methods. Bring huge losses to our economy every year

Method used

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  • Use of black mulberry extract as tyrosinase inhibitor
  • Use of black mulberry extract as tyrosinase inhibitor
  • Use of black mulberry extract as tyrosinase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Determination of compound (I) tyrosinase inhibitory activity.

[0026] The determination of tyrosinase inhibitory activity takes kojic acid as a reference substance, and the method is as follows:

[0027] The substrate dopa was first dissolved in phosphate buffer (25 mM, pH 6.8) to a concentration of 8 mM. To a 96-well plate, 20 μL of the above substrate solution, 90 μL of phosphate buffer, and 20 μL of compounds of different concentrations were added. Add 70 μL of tyrosinase-containing phosphate buffer (100 U / mL, pH 6.8) to start the reaction, and react at 37° C. for 10 minutes. Before and after the reaction, the absorbance of each well was measured with a Multiscan MK3 automatic multifunctional microplate reader (Thermo) at 492 nm.

[0028] According to the absorbance at 492nm, the inhibitory rate (%) of the compound to tyrosinase is calculated, and the concentration of the inhibitor when the enzyme activity inhibitory rate (%) reaches 50% is determined a...

Embodiment 2

[0038] Example 2: Kinetic analysis of tyrosinase inhibition.

[0039] The substrate dopa was dissolved in phosphate buffer (25mM, pH6.8) and prepared into 4 concentrations of 12mM, 10mM, 8mM and 6mM. Into the 96-well plate, 20 μl of the above-mentioned substrate solutions of different concentrations, 90 μl of phosphate buffer, and 20 μl of compounds of different concentrations were added. Add 70 μL of tyrosinase-containing phosphate buffer (100 U / mL, pH 6.8) to start the reaction, and react at 37° C. for 10 minutes. Before and after the reaction, the absorbance of each well was measured at 492 nm. Calculate enzyme inhibition rate, method is with embodiment 1, draws Lineweaver-Burk curve (see figure 2 ).

[0040] Conclusion: from figure 2 It can be seen from the Lineweaver-Burk curve that compound (I) is a competitive inhibitor.

Embodiment 3

[0041] Example 3: Time Analysis of Tyrosinase Inhibition.

[0042] The substrate dopa was first dissolved in phosphate buffer (25 mM, pH 6.8) to a concentration of 8 mM. To a 96-well plate, add 20 μL of the above substrate solution, 90 μL of phosphate buffer, and 20 μL of the compound at a final concentration of 4 μM. Add 70 μL of tyrosinase-containing phosphate buffer (100 U / mL, pH 6.8) to start the reaction, and measure the absorbance of each well at different times. Calculate enzyme inhibition rate, method is the same as embodiment 1.

[0043] The results are shown in Table 2.

[0044] Table 2: Tyrosinase inhibitory activity at different times

[0045] time (min) Inhibition rate(%) 5 60.69±3.32 10 70.71±0.30 15 74.62±2.19 30 73.11±2.42 60 69.47±2.77 90 68.97±3.05 120 66.80±3.42 150 65.88±3.43 180 62.82±2.47

[0046] Conclusion: Compound (I) had an enzyme inhibition rate of 74.62% when reacted for 15 minutes. A...

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Abstract

The invention relates to an application for extracting natural compound (I) from black mulberry, the compound (I) has strong inhibition effect for tyrosinase and is applied for preventing skin mottle, freckle, senile plaque or the like skin chromatosis and whitening, and the compound (I) is a food additive. The activity of the compound (I) is strong proved by the mensuration of the inhibition activity, the compound (I) has good stability and obvious growth inhibition action for the melanoma cell, can reduce the activity of the tyrosinase in the melanoma cell and biological synthesis level of the melanin in the cell.

Description

technical field [0001] The present invention relates to a black mulberry extract, in particular to the use of a natural compound extracted from the black mulberry. Background technique [0002] With the improvement of people's living standards and aesthetic standards, people's requirements for healthy and whitening skin are increasing day by day. Therefore, in recent years, more and more attention has been paid to the development of the skin whitening cosmetics market. Whitening cosmetics have become one of the mainstream varieties of skin care cosmetics, and their consumer market is constantly expanding. [0003] Studies have shown that skin pigmentation is caused by excessive melanin production in melanocytes, and tyrosinase is an essential key enzyme in the biosynthesis of melanin. Therefore, by inhibiting the activity of tyrosinase, melanin production can be limited and skin pigmentation can be improved. [0004] At present, many kinds of tyrosinase inhibitors have bee...

Claims

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Application Information

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IPC IPC(8): A61K31/047A61K8/35A61K8/34A61K8/49A61K8/60A61K8/67A61K8/97A61P17/00A61Q19/00A61Q19/04A23L3/349
Inventor 王贺瑶侯爱君张小东胡晓
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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