11 results about "Enzyme inhibition" patented technology

The application discloses a preparation method of ethanol extract of Drynaria Rhizome and application thereof. The method comprises the following steps: crushing and sieving Drynaria Rhizome, adding ethanol, reflux extraction, combining filtrates, vacuum concentration and drying, and thus the ethanol extract of Drynaria Rhizome is obtained. The in-vitro alpha-glucosidase enzyme inhibition experiment shows that the ethanol extract of Drynaria Rhizome has a significant inhibitory effect on alpha-glucosidase enzyme, and the IC 50 is about 142.68 μ g / mL. Therefore, the ethanol extract of Drynaria Rhizome can be used for preparing a hypoglycemic drug, health care product or functional food, and has a wide application prospect.

The application provides a whole-process cold-chain preservation method for fresh large yellow croaker, which is targeted to enzyme inhibition and gradient microenvironment regulation. The method provided by the application comprises the following steps: 1, soaking the freshly caught large yellow croaker in seawater containing endogenous protease inhibitor peptides of the large yellow croaker within 30 minutes after catching, and reducing the core temperature of the fish body to 3-4 DEG C; after the soaking is completed, the surface moisture of the fish body is blown dry with sterile wind; 2, packaging the large yellow croaker after the surface moisture of the fish body is blown dry, and storing the packaged large yellow croaker in an environment with a temperature of-0.5-1 DEG C and a relative humidity of 65%-75%, so as to realize the preservation of the fresh large yellow croaker. The method provided by the application can greatly improve the preservation effect, and the endogenous protease inhibitor peptides of the large yellow croaker do not destroy the nutritional components of the fish meat, do not change the original flavor, have no chemical preservative residues, meet the green food standard, and are safer than the existing chemical preservation and soaking preservation technologies.

The application belongs to the technical field of pharmaceutical chemistry, and particularly relates to a gentiopicroside nitroxide radical derivative as well as a preparation method and application thereof. A series of gentiopicroside nitroxide radical derivatives with specific structures are prepared and synthesized through a chemical process. The derivatives exhibit good in-vitro COX-2 enzyme inhibition activity and in-vitro ROS scavenging activity. In-vivo and in-vitro experimental results show that each derivative can effectively inhibit the release of inflammatory mediators and oxidative stress during inflammation, thereby more effectively inhibiting the progress of inflammation and protecting tissues, and the derivatives have a wide application prospect in the preparation of drugs with the efficacy of preventing, relieving or treating inflammation.

The application discloses arginase 2 inhibiting peptides and application thereof in wound healing. The arginase 2 inhibiting peptide has an amino acid sequence as shown in SEQ ID No. 1 or a mutant sequence thereof. It is found through research of the application at a cell level and in a mouse skin wound model that MUT4-short peptide and MUT5-short peptide can effectively improve the viability and migration ability of keratinocytes under high-sugar conditions in vitro and in a metabolic abnormality model in vivo, reduce the intracellular reactive oxygen level, and promote the repair process of skin wounds, wherein the MUT4-short peptide shows more significant in promoting the recovery of cell functions and accelerating wound healing, and has a good application prospect in promoting skin wound healing, especially in the treatment of difficult-to-heal wounds related to metabolic diseases such as diabetes.

The application relates to the technical field of daily chemical products, and discloses a porous carrier-loaded organic zinc slow-release deodorizing composition and a preparation method and application thereof, wherein the organic zinc slow-release deodorizing composition comprises the following components in percentage by mass: organic zinc pre-loaded on a porous carrier, wherein the mass percentage of the organic zinc is 8-25%, and the mass percentage of the porous carrier is 3-25%; a physical embedding type synergist: 1-10%; an enzyme inhibition type synergist: 0.5-8%; and the balance is water. The composition provided by the application realizes a 12-hour or longer sustained deodorizing effect through a multiple synergistic mechanism of porous carrier slow release, physical embedding capture of odor molecules and enzyme inhibition reduction of odor precursor generation, and is suitable for human body, pets, oral cavity, household environment and textile deodorizing finishing fields.

This invention provides a polysaccharide from *Melia azedarach*, its extraction method, and its applications, belonging to the field of polysaccharide extraction technology. The *Melia azedarach* polysaccharide includes at least one of *Melia azedarach* polysaccharide 1 and *Melia azedarach* polysaccharide 2. *Melia azedarach* polysaccharide 1 comprises the polysaccharide molecule represented by formula (I), and *Melia azedarach* polysaccharide 2 comprises the polysaccharide molecule represented by formula (II). This invention also provides a method for extracting this *Melia azedarach* polysaccharide. This *Melia azedarach* polysaccharide exhibits good effects in fat binding, bile acid binding, cholesterol binding, and lipase inhibition, and has broad application prospects in the preparation of lipid-lowering products.

This invention provides naphthoquinone compounds targeting the novel coronavirus and their pharmaceutical uses. In vitro activity assays show that the compounds disclosed in this invention can inhibit the main protease (Mpro) activity of the SARS-CoV-2 novel coronavirus. In vitro activity assays show that some compounds, at a concentration of 1 μM, exhibit an enzyme inhibition rate of over 90%, significantly superior to the positive control drug shikonin, and can effectively reduce the replication capacity of wild-type and various mutant strains of the novel coronavirus in the host. In vitro cytotoxicity assays show that the compounds disclosed in this patent have significantly lower toxicity to normal host cells (HSF cells) than the positive control drug shikonin, indicating promising application prospects as drug candidates against COVID-19 infection.

This invention relates to the fields of food flavor science and functional food ingredients, and provides a small molecule bitter peptide and its applications. The amino acid sequence of the small molecule bitter peptide is: Ala-Cys-Trp-Asp-Ala-Glu-Glu-Leu-Glu-Arg-Lys (ACWDAEELERK), exhibiting excellent water solubility and displaying bitter characteristics in sensory evaluation and electronic tongue testing. In vitro enzyme inhibition experiments show that this peptide has a significant inhibitory effect on both α-amylase and α-glucosidase, with a half-inhibitory concentration (IC50) for α-amylase. 50 The half-maximal inhibitory concentration (IC50) for α-glucosidase was 127.12 μg / mL. 50 The concentration was 2.75 mg / mL. The small molecule bitter peptides provided by this invention have both flavor activity and biological activity, and have potential application value in the development of functional foods.

The application discloses a soil heavy metal analysis and determination method based on an enzyme inhibition method, and comprises the following specific steps: S1, taking soil: a certain amount of soil to be detected is taken for standby; S2, placing into a mixing tube: the soil is quantitatively placed into a mixing tube; S3, preparing a detection solution: the soil is mixed with water, a buffer solution and an enzyme in the mixing tube to prepare a detection solution for standby; S4, color development: the detection solution is directly subjected to preliminary color development in the mixing tube; and S5, colorimetric detection by a detector: the buffer solution in the mixing tube is subjected to fine colorimetric detection by using the detector; the mixing tube placed into the mixing tube in the step S2 comprises a soil taking tube, a top cover is covered on the soil taking tube, and a shaking mechanism is connected to the top end of the top cover, the soil heavy metal analysis and determination method based on the enzyme inhibition method can directly avoid the step of outdoor weighing, is simple to operate, and can guarantee the accuracy of detection results.

This application relates to the field of extraction of effective components from woody plants, and particularly to a method and application for the preparation of abalone fruit oil, abalone fruit protein, abalone fruit active protein peptides, and their co-production. This method achieves efficient preparation of the target products: firstly, specific infrared-assisted technology is used to efficiently extract oil from abalone fruit, improving the oil extraction rate and purity; subsequently, a calcium hydroxide-phosphate system is used to directionally separate and extract proteins from the remaining raw materials, ensuring the structural integrity and extraction efficiency of the proteins, and exhibiting good emulsification stability; finally, using the extracted proteins as substrates, directional hydrolysis is performed via enzymatic hydrolysis to prepare selenium / zinc-enriched abalone fruit active peptides with good acetylcholinesterase / lipase inhibitory activity. This preparation process is rational, simple to operate, efficient, and environmentally friendly, achieving comprehensive utilization of abalone fruit resources. The prepared active peptides possess multiple advantages, including zinc / selenium enrichment and bioactivity, and the preparation process is suitable for industrial production.