108 results about "Enzyme inhibition" patented technology

The invention discloses a xanthine oxidase inhibitory peptide with a kidney improving function and application thereof, and belongs to the technical field of biological medicines.The xanthine oxidase inhibitory peptide is screened out by conducting enzymolysis, separation and purification on litopenaeus vannamei processing by-products and utilizing a molecular docking technology, the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Leu-Gly-Pro-Pro-Pro, and the amino acid sequence of the xanthine oxidase inhibitory peptide is Pro-Leu-Gly-Pro-Pro-Pro. And the half inhibition concentration of the polypeptide is 2.356 + / -0.2448 mM. The xanthine oxidase inhibitory peptide is proved to have an improvement effect on hyperuricemia mice, the improvement effect is mainly characterized by reducing the content of serum uric acid, creatinine and urea nitrogen of the hyperuricemia mice, inhibiting the activity of xanthine oxidase (XOD) in serum and livers of the mice, inhibiting synthesis of uric acid and improving kidney injury of the mice, and Toxinpred prediction shows that the xanthine oxidase inhibitory peptide has the effect of inhibiting the activity of xanthine oxidase (XOD) in the serum and livers of the mice. The xanthine oxidase inhibitory peptide is free of toxic and side effects, so that the xanthine oxidase inhibitory peptide has a wide application prospect in preparation of food or drugs for improving renal functions.

The invention provides a method for preparing high-purity phosphatidylserine by a batch enzyme catalysis method based on dynamic choline removal, and belongs to the technical field of enzymatic reaction. According to the method disclosed by the invention, in a reaction system for preparing phosphatidylserine, a byproduct choline is selectively adsorbed by adopting resin. Choline in a reaction system is removed in real time through the integrated adsorption separation module, the enzyme inhibition problem is solved, and meanwhile, the PC conversion rate is larger than or equal to 98% and the PS purity is larger than or equal to 96% in combination with the immobilized enzyme circulation technology; the enzyme can be reused for more than or equal to 20 times (activity retention is more than or equal to 90%); and the choline residual concentration is less than or equal to 0.5 mM (an inhibition threshold value or less).

The invention relates to the technical field of agriculture, in particular to a Raman spectrum and deep learning-based pesticide residue detection method and electronic equipment. The method comprises the following steps: acquiring Raman spectrum data of a sample to be detected; inputting the Raman spectrum data into a pre-trained qualitative analysis model, and outputting the category probability of the pesticide components in the to-be-detected sample; inputting the Raman spectrum data into a pre-trained quantitative analysis model, and outputting the content proportion of the pesticide components in the to-be-detected sample; wherein the qualitative analysis model realizes conjoint analysis of spectral global features and local features based on a multi-modal fusion deep learning network; the quantitative analysis model realizes spectral decomposition and concentration regression based on a deep learning architecture of multi-scale feature fusion. The detection defects of an enzyme inhibition method and a chromatography method are overcome, the problem that the potential mode of the Raman spectrum cannot be accurately and stably found through machine learning is solved, and higher detection precision and higher detection speed are achieved.

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention relates to a pesticide residue detection test strip as well as a preparation method and application thereof, in particular to a rapid detection technology based on an enzyme inhibition method and a chitosan non-woven fabric carrier. The test strip can specifically detect organophosphorus and carbamate pesticides such as trichlorfon, malathion and phoxim through the enzyme activity inhibition principle, the detection limit of the pesticides such as phoxim is as low as 0.1 mg / kg, the developing intensity is in negative correlation with the pesticide concentration, and semi-quantitative judgment can be achieved. The preparation process is simple, the chitosan non-woven fabric is adopted as a fixed carrier, the material is environment-friendly and degradable, the enzyme activity recovery rate reaches 90%, the stability is excellent (the enzyme activity recovery rate can be preserved for 60 days at the temperature of 4 DEG C in a dark place), sample pretreatment is not needed in detection operation, a result can be obtained at the normal temperature for 15 min, the stability and repeatability are good, and the problems that a traditional instrument is high in detection cost and long in time consumption are solved; the method has a remarkable basic popularization value.

The invention discloses a preparation method of deer spleen aminopeptide powder in the field of bioactive substance extraction and functional material compositing, and the performance of the deer spleen aminopeptide powder is improved by compositing a multi-stage functional modified material and deer spleen aminopeptide. The preparation method comprises the following steps: cleaning, homogenizing and crushing fresh deer spleen, extracting with a phosphate buffer solution, adjusting the pH value, carrying out enzymolysis, inactivating enzyme, and treating with an ultrafiltration membrane and a nanofiltration membrane in a specific molecular weight range to obtain a concentrated solution; and mixing the concentrated solution with a multi-stage functional modified material under proper conditions, sterilizing, pre-freezing, and freeze-drying to obtain the deer spleen aminopeptide powder. Wherein the modified material is constructed through quaternization modification, nanopore loading, enzyme inhibitor coupling and targeting ligand modification, and has the functions of charge repulsion, steric hindrance, enzyme inhibition and targeting recognition. The deer spleen aminopeptide powder prepared by the invention has high stability and good bioavailability, and is suitable for functional food or health care products.

The invention relates to a diatomic catalyst for detecting carbosulfan pesticide residue and a preparation method, and belongs to the technical field of food safety detection. According to the invention, a reducing sulfide generated by breaking a nitrogen-sulfur (N-S) bond of carbosulfan under an acidic condition is used as a specific substance. The reductive sulfide not only can prevent bimetal active sites in the diatomic catalyst, but also consumes hydroxyl radicals generated by catalyzing hydrogen peroxide (H2O2), and the sensing switch enzyme formed by a dual enzyme inhibition mechanism causes the enzyme substrate 3, 4-dihydroxyl-3, 4-dione to be converted into the diatomic catalyst, so that the catalytic activity of the diatomic catalyst is improved. The blue color of the 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) gradually becomes light along with the increase of the concentration of carbosulfan, sensitive visual detection is implemented, and after a blue product is read by using an ultraviolet spectrophotometer, the absorbance value generated by an ultraviolet absorption spectrum at 654 nm is linearly dependent on carbosulfan. The prepared diatomic catalyst effectively improves the detection sensitivity of the carbosulfan pesticide residue, and has good popularization and application prospects.

The invention discloses preparation of immobilized alpha-glucosidase based on a magnetic metal organic framework (MOF) and application of the immobilized alpha-glucosidase in screening of natural enzyme inhibitors. According to the method, ZIF-90 is grafted on the surfaces of Fe3O4 magnetic nanoparticles modified by codeposition of polydopamine and polyethyleneimine, and alpha-glucosidase is synchronously encapsulated in situ in the growth process of the ZIF-90, so that the magnetic MOF immobilized alpha-glucosidase is successfully constructed. On the application level, an in-vitro enzyme inhibition activity evaluation system is constructed, multiple traditional Chinese medicine extracts are selected as research objects, and by means of the characteristic that the magnetic MOF immobilized alpha-glucosidase is easily separated from a reaction system, accurate screening of natural inhibitors or active small molecules is rapidly and efficiently achieved; and a novel screening method is provided for research and development of candidate drugs for diabetes mellitus. The method comprises the following steps: 1, preparing the magnetic MOF immobilized alpha-glucosidase and evaluating the performance of the magnetic MOF immobilized alpha-glucosidase; and 2, the prepared immobilized alpha-glucosidase is used for screening a natural enzyme inhibitor. Based on a magnetic MOF immobilized enzyme technology, the method provided by the invention has the characteristics of rapidness, high efficiency, accuracy, easiness in separation from a reaction system and reutilization of a target receptor alpha-glucosidase, is suitable for screening of alpha-glucosidase inhibitors in complex systems such as traditional Chinese medicines, and has a wide application prospect.

The invention relates to a small cellar sauced vinasse hydrolysis peptide with alpha-glucosidase inhibitory activity and a preparation method thereof. Five small cellar Maotai-flavor vinasse hydrolytic peptides with alpha-glucosidase inhibitory activity are prepared through vinasse protein preparation, enzymolysis, separation and purification and identification, and the sequences of the five small cellar Maotai-flavor vinasse hydrolytic peptides are AAPFYAL, LAYPQ, IRLPFNQI, INPALPW and ATPVFL respectively. The molecular docking analysis result shows that the interaction between the vinasse polypeptide and the amino acid residues of the alpha-glucosidase active pocket is mainly based on hydrogen bonds, electrostatic binding hydrophobic interaction and pi-pi interaction. Pharmacophore construction and matching analysis show that the vinasse polypeptide has a common characteristic structure composed of a positive ion center and three hydrogen bond donors and plays a key role in enzyme inhibition. The small cellar sauced vinasse polypeptide with alpha-glucosidase inhibitory activity prepared by the invention can be used for developing health-care foods and medicines for reducing blood sugar.

The invention discloses a fruit and vegetable pesticide residue rapid screening method based on an enzyme inhibition ratio method, and relates to the technical field of pesticide detection.The method comprises the following steps that S100, visible light, near-infrared and hyperspectral data of fruit and vegetable samples are synchronously collected through a multispectral sensing module, and a pH adjusting module is dynamically triggered based on spectral characteristic peak intensity; s200, executing dynamic pH regulation and enzyme reaction monitoring based on the output of the step S100; s300, based on the output of the S200, fusing the multispectral data and the dynamic curve through an intelligent analysis platform, outputting a pH sensitivity response curved surface through a transfer learning module, and dynamically updating proportional-integral control parameters; and S400, establishing a feedback verification closed loop based on a feedback module, automatically generating a unique identification code and triggering a GC-MS laboratory for confirmation when a positive sample of which the inhibition ratio is greater than a preset value is detected, and finally, synchronously and reversely calibrating the spectrum fusion weight in S300 and the pH adjustment parameter in S200 based on the deviation value.

The invention discloses a rapid screening and detecting method for vegetable pesticide residues, a multi-dimensional judgment basis is provided for pesticide residue detection by combining a PCR amplification result with the inhibition rate of acetylcholin esterase and butyrylcholin esterase, different types of pesticides may induce different gene expression changes, and the detection result is accurate. According to the rapid screening and detecting method for the pesticide residues in the vegetables, the accuracy of a detection result is improved, meanwhile, the variety of the pesticide residues in the vegetables can be accurately determined, the defect that specific pesticide varieties are difficult to distinguish by purely relying on an enzyme inhibition method is overcome, and the detection result is more targeted and accurate.

The invention relates to the technical field of preparation of electrochemical sensors, and particularly discloses a preparation method of a novel electrochemical enzyme sensor for profenofos pesticide detection based on an enzyme inhibition action principle, and the preparation method comprises the following steps: S1, treating a glassy carbon electrode to obtain a bare glassy carbon electrode GCE; s2, obtaining a composite material modified electrode Au50Ag2 (at) G / GCE; s3, an enzyme-chitosan composite modified electrode KbE-CS / Au50Ag2 (at) G / GCE is obtained; and S4, finally obtaining the profenofos electrochemical enzyme sensor NF / KbE-CS / Au50Ag2 (at) G / GCE based on the enzyme inhibition effect. According to the present invention, the white kidney bean esterase derived from plants is adopted as the detection enzyme source, and the electro-catalysis amplification effect of the Au50Ag2 (at) G composite material is adopted to construct the novel electrochemical enzyme sensor, and the method is the profenofos pesticide detection technology based on the enzyme inhibition effect principle;

The application discloses a preparation method of ethanol extract of Drynaria Rhizome and application thereof. The method comprises the following steps: crushing and sieving Drynaria Rhizome, adding ethanol, reflux extraction, combining filtrates, vacuum concentration and drying, and thus the ethanol extract of Drynaria Rhizome is obtained. The in-vitro alpha-glucosidase enzyme inhibition experiment shows that the ethanol extract of Drynaria Rhizome has a significant inhibitory effect on alpha-glucosidase enzyme, and the IC 50 is about 142.68 μ g / mL. Therefore, the ethanol extract of Drynaria Rhizome can be used for preparing a hypoglycemic drug, health care product or functional food, and has a wide application prospect.

The application provides a whole-process cold-chain preservation method for fresh large yellow croaker, which is targeted to enzyme inhibition and gradient microenvironment regulation. The method provided by the application comprises the following steps: 1, soaking the freshly caught large yellow croaker in seawater containing endogenous protease inhibitor peptides of the large yellow croaker within 30 minutes after catching, and reducing the core temperature of the fish body to 3-4 DEG C; after the soaking is completed, the surface moisture of the fish body is blown dry with sterile wind; 2, packaging the large yellow croaker after the surface moisture of the fish body is blown dry, and storing the packaged large yellow croaker in an environment with a temperature of-0.5-1 DEG C and a relative humidity of 65%-75%, so as to realize the preservation of the fresh large yellow croaker. The method provided by the application can greatly improve the preservation effect, and the endogenous protease inhibitor peptides of the large yellow croaker do not destroy the nutritional components of the fish meat, do not change the original flavor, have no chemical preservative residues, meet the green food standard, and are safer than the existing chemical preservation and soaking preservation technologies.

The invention relates to the technical field of microbial fermentation, and discloses a fermentation composition with a whitening effect and a preparation method and application thereof. According to the application, Chinese rose, a sodium glutamate culture medium and sprouted mung beans are respectively used as fermentation objects, and are fermented by selecting plant lactobacillus and bacillus subtilis to respectively obtain a Chinese rose fermented product, a glutamic acid fermented product and a mung bean sprout fermented product; compared with the prior art, the arbutin fermented product has the advantages that the arbutin fermented product shows more excellent monophenol enzyme inhibition rate and diphenol enzyme inhibition rate which are superior to those of arbutin, particularly, the effect is optimal when the three fermented products are compounded, and a theoretical basis is provided for development and application of whitening cosmetic raw materials and products.

The invention relates to the technical field of neutrophile granulocyte separation, in particular to a mouse neutrophile granulocyte separation method which comprises the following steps: (1) anesthetizing and disinfecting a mouse; (2) tissue sampling; (3) tissue digestion; (4) preparing a cell suspension; (5) purifying cells; (6) cell culture; according to the method disclosed by the invention, isoflurane is adopted in the anesthesia disinfection step, so that central nervous activity of mice can be rapidly inhibited, tissue damage caused by stress reaction is avoided, and chemical stimulation can be reduced while body surface microorganisms are cleared through combination of ethanol disinfection and sterile normal saline flushing, and aortic tissues with intact activity are provided for subsequent material taking. In the compound enzyme digestive juice, collagenase I can specifically degrade aortic wall collagenous fibers, trypsin dissociates adhesion connection between cells, neutral protease assists in decomposing elastic fibers, and DNA enzyme I is supplemented to inhibit cell aggregation, so that the contradiction between incomplete digestion and large cell damage of the traditional enzyme juice is effectively solved.

The invention discloses a genetically engineered bacterium for synthesizing N-acetyl blue as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium is prepared by knocking out a lactic dehydrogenase gene ldh and inserting a phosphoketolase gene xfpK on a knockout site, knocking out a pyruvate oxidase gene poxB and inserting a phosphate transacetylase gene pta on a knockout site of a microorganism for producing N-acetyl blue, and knocking out an aconitase inhibition factor gene acnR, inserting a glutamate dehydrogenase gene gdhA on a knockout site, knocking out a glutamine synthetase adenylation transferase gene glnE, and inserting an alpha-ketoglutarate dehydrogenase inhibition factor gene odhI on a knockout site to obtain the strain. According to the method, the supply of the precursor acetyl coenzyme A is enhanced by introducing a non-oxidative glycolysis pathway, the pH dynamic regulation system balances the metabolic flux, and the double breakthrough of the yield and the carbon recovery rate of the N-acetyl blue is realized by combining a two-stage fermentation process.

The invention discloses a low-sensitivity whitening recombinant humanized collagen composition and a preparation method thereof. The low-sensitivity whitening recombinant humanized collagen composition is prepared from modified recombinant humanized collagen, tetrahydrocurcumin, ethyl ascorbic acid ether and dipotassium glycyrrhizinate, the modified recombinant humanized collagen comprises a recombinant humanized collagen chain segment, a polyethylene glycol chain segment modified on the recombinant humanized collagen chain segment, and tetrahydromethyl pyrimidine formyl modified at the other end of the polyethylene glycol chain segment. The modified recombinant humanized collagen can provide proper steric hindrance to achieve the effects of shielding residual antigen epitopes and improving the efficiency of combining target spot cell surface receptors, and cooperates with other mild whitening components to act on various pathways (including oxidation resistance, transport inhibition, enzyme inhibition and inflammation resistance), so that synergistic interaction and irritant hedging are realized, and the skin whitening effect is improved. Therefore, the whitening effect is achieved under low skin irritation.

An instant fish maw processing technology for delaying degradation of collagen belongs to the technical field of instant fish maw processing, and comprises the following steps: S1, treating fish maw with 0.1% of tea polyphenol, 0.05% of a rosemary extract and 0.2% of chitosan oligosaccharide for 30 min, carrying out enzyme inhibition treatment, and then cleaning and draining for later use; s2, dipping the fish gelatin treated in the step S1 in a solution containing 0.1% of riboflavin and 0.2% of hyaluronic acid, irradiating the fish gelatin with white light for 10s, and then cleaning and draining the fish gelatin for later use; and S3, putting 5-7 parts by weight of fish maw and 65-80 parts by weight of pure milk into a container, sealing, and sterilizing to obtain the instant fish maw. Through enzyme inhibition treatment, the hydrolysis capability of metalloproteinase on collagen is reduced or eliminated, and degradation of the collagen is reduced, so that the nutritional value of the fish maw is maintained; collagen molecules in the fish maw and riboflavin are subjected to a cross-linking reaction to form a stable three-dimensional network structure, so that the acting force between the collagen molecules is enhanced, the structural stability of the collagen is improved, and the degradation of the collagen is delayed.

A method for estimating an enzyme inhibition constant, according to one embodiment of the present disclosure, may comprise the steps of: measuring initial reaction rate data at a plurality of substrate concentrations and one inhibitor concentration; setting the one inhibitor concentration to be greater than or equal to a predetermined half-maximal inhibitory concentration; and using the measured initial reaction rate data and the predetermined half-maximal inhibitory concentration value so as to estimate an inhibition constant by fitting to an enzyme inhibition model and applying a normalization term that includes the relationship between the half-maximal inhibitory concentration and at least one candidate inhibition constant.

The invention relates to the technical field of marine food preparation, and discloses minced Antarctic krill with a high astaxanthin retention rate and a preparation method thereof.The preparation method comprises the steps that Antarctic krill is pretreated and then soaked in composite antioxidant liquid for composite antioxidant treatment, and then enzyme inhibition liquid is atomized and sprayed for low-temperature enzyme inhibition treatment; grinding at low temperature to obtain minced shrimps; and mixing the minced shrimps with the anti-oxidation emulsion, carrying out secondary anti-oxidation treatment, and then carrying out freezing and shaping. The euphausia superba is subjected to anti-oxidation treatment through the composite anti-oxidation liquid and the anti-oxidation emulsion, and the problem that the retention rate of astaxanthin is low due to the fact that the astaxanthin is sensitive to oxygen and metal ions and prone to oxidation is solved; meanwhile, the low-temperature enzyme inhibition treatment improves the elasticity and the viscosity of the minced shrimps and also reduces the loss of protease-mediated astaxanthin; the low-temperature grinding treatment not only protects the molecular structure integrity of astaxanthin, but also improves the separation rate of crustacean fragments.

The invention discloses a preparation method and application of a nano-material for inhibiting BACE1 enzyme activity, nobiletin entrapment is realized through electroporation of stem cell exosome under the condition of a pulsed electric field, the nano-material for inhibiting BACE1 enzyme activity is obtained, and the nano-material has high safety and BACE1 enzyme inhibition capability, and provides a new choice for treating AD (Alzheimer's disease).

The invention discloses a Chinese yam cake with a compound sour system for synergistically activating salivary amylase and a preparation method thereof. The Chinese yam cake comprises the following raw materials: Chinese yam, konjaku flour or oligosaccharide, a sweetening agent, a gelling agent, malic acid, citric acid, hawthorn, dark plum and water. The main structure matrix is jointly constructed by Chinese yam powder, konjaku flour / oligosaccharide and gels to form a cake body base; a composite sour system is uniformly distributed in a gel network, a complementary system is formed through superposition of various organic acids, and the composite sour system has good buffer capacity, can maintain weak acidic pH in the oral cavity and activate the activity of alpha-amylase without inhibition, and is different from the problem that a single acid system is prone to'excessive acid 'enzyme inhibition; and an optimal reaction microenvironment is provided for salivary enzyme activity, and multi-level taste experience is also provided. The gel characteristics of Chinese yam mucoprotein and oligosaccharide are fused to form a sour slow-release matrix, so that sour release presents a dynamic curve of front fast-middle stable-rear slow, the flavor release time is prolonged, and the continuous perception of the oral cavity is enhanced. The enzymatic reaction in the oral cavity is prolonged.

The invention relates to a sugar-controlling and weight-losing functional food cooperatively conditioned by endogenous enzymes of processing raw materials and a preparation method thereof, and belongs to the technical field of food. The raw materials comprise 6-10 parts of white kidney beans, 4-8 parts of lucid ganoderma, 20-40 parts of black-skin and green-kernel black beans, 3-6 parts of mung beans and 40-60 parts of wheat flour / rice flour. A dual biological activation process is adopted: white kidney beans are subjected to low-temperature enzymolysis by yeast powder and probiotic soybean enzymes (1%, 25-35 DEG C) for 2-4 hours to release an alpha-amylase inhibitor, and ganoderma lucidum is subjected to weak-base hydrolysis by sodium bicarbonate (1-3%) for 2-4 hours to activate a triterpenoid glycosidase inhibitor; and performing compound fermentation on the zymolyte and the alkaline hydrolysis product for 2-4 hours, and then adding a cereal base material to activate the endogenous enzyme activity. High fiber combination (more than 15%) is used for prolonging satiety and reducing fat absorption, resistant starch (heat is only 50% of saccharides) is used for inhibiting lipid synthesis, and quadruple synergy (enzyme inhibition is more than 90% / resistant starch / low GI / high fiber) is combined, so that dual effects of postprandial blood sugar reduction and weight control are realized.

The invention provides a method and equipment for screening xanthine oxidase inhibitory peptides. The method comprises the following steps: acquiring a first peptide fragment with xanthine oxidase inhibitory activity as a positive sample, and acquiring a second peptide fragment without activity as a negative sample; respectively carrying out activity labeling; performing quantization coding on the two peptide fragments by adopting amino acid physicochemical property descriptors to generate an initial feature matrix, and performing pooling operation on the matrix to obtain a fixed-length peptide fragment feature vector; training an ensemble learning classification model to obtain a xanthine oxidase inhibitory peptide prediction model by taking the fixed-length peptide fragment feature vector as input and the activity label as output; quantizing and coding the candidate peptide fragment set and then inputting the candidate peptide fragment set into the prediction model to obtain an inhibitory activity prediction probability; and screening out a xanthine oxidase inhibitory peptide candidate sequence with high confidence from the candidate peptide fragment set. Based on the method, the invention further provides screening equipment of the xanthine oxidase inhibitory peptide. According to the method, a large number of candidate peptide fragments can be quickly predicted, and a high-confidence candidate sequence is obtained.

The invention discloses a preparation method of an electrochemical sensor for organophosphorus pesticide residues in traditional Chinese medicinal materials based on AuNPs / BiVO4, and relates to the field of electrochemical sensors, the method comprises preparation of a BiVO4 / GCE modified electrode, preparation of an AuNPs / BiVO4 / GCE modified electrode, and preparation of an AChE / PANI / AuNPs / GCE electrochemical sensor. According to the invention, BiVO4 and AuNPs are used as electrode substrate materials to load acetylcholin esterase (AChE), so that the electrochemical enzyme biosensor which takes AChE as a recognition element and has high sensitivity, high selectivity, recognition and fast detection is constructed, and the electrochemical enzyme biosensor is used for detecting organophosphorus pesticide residues in traditional Chinese medicinal materials. The sensor detects Ops by using an enzyme inhibition principle, shows higher detection sensitivity and wider detection range, shows high stability and reproducibility in detection of organic phosphorus pesticide residues in traditional Chinese medicinal materials, and provides a more effective technical means for safety monitoring of the traditional Chinese medicinal materials.

The utility model discloses an automatic enzyme inhibition ratio method pesticide residue detection device which comprises a vibration separation assembly, a liquid adding and transferring assembly, a constant temperature shaking assembly and a spectrum detection assembly, the vibration separation assembly is used for preparing a sample extracting solution, the sample extracting solution is transferred to the constant temperature shaking assembly through the liquid adding and transferring assembly, then enzyme and a color developing agent are added, and the spectrum detection assembly is used for detecting the pesticide residue. And after constant-temperature culture is completed, transferring to a spectrum detection assembly for detection. After a sample is placed in the device, the preparation of a sample extracting solution is automatically completed; enzyme adding, color developing agent adding and constant-temperature culture operations of a sample extracting solution are automatically completed; and the spectral analysis operation of the sample extracting solution is automatically completed.

The present invention discloses a pinanyl fluorescent probe for detecting the organophosphorus pesticide methyl parathion based on an enzyme inhibition mechanism, as well as its preparation method and application. The fluorescent probe is: 4-(2-(4-(cyclopropylformyloxy-4′-(6,6-dimethyl-2-(4-nitrophenyl)-4,5,6,7-tetrahydro-2H-5,7-methyleneindazole-3-yl)-[1,1′-biphenyl]-3-yl)vinyl)-1-methylpyridinium iodide (abbreviated as: THIP-OCP). The compound can undergo enzymatic hydrolysis reaction with butyrylcholinesterase and, under irradiation with ultraviolet light at a wavelength of 365nm, The solution's fluorescence color changes from colorless to orange-yellow. Since methyl parathion effectively inhibits butyrylcholinesterase activity, when methyl parathion is added to the solution, the solution's fluorescence gradually changes from orange-yellow to colorless under ultraviolet irradiation at a wavelength of 365 nm. Therefore, this compound can be used as a fluorescent probe for detecting methyl parathion. This compound has many advantages, including a low detection limit (0.79 μg / mL) and a wide pH range (5-10), and has great application prospects.

The invention belongs to the field of pesticides, and particularly relates to a novel glycosyl C-glycoside derivative as well as a preparation method and application thereof, and the structure of the novel glycosyl C-glycoside derivative is shown as a formula I or a formula II; the compound can be used as an insect chitin degrading enzyme, and has good target enzyme inhibition activity and insecticidal activity when being used for preventing diseases and insect pests caused by lepidoptera insects. # imgabs0 #

The invention belongs to the technical field of biological medicine, and particularly relates to an N-aryl substituted amide as a novel HDAC6 (histone deacetylase 6) inhibitor and application of the N-aryl substituted amide in preparation of antitumor drugs. A compound shown as a formula (I) is screened and synthesized on the basis of an artificial intelligence technology; the compound shows excellent inhibitory activity on HDAC6, and can be used for efficiently inhibiting HDAC6 and / or HDAC1; in addition, anti-tumor cell proliferation evaluation further reveals that the compound can significantly inhibit proliferation of tumor cells. Based on the excellent enzyme inhibition activity and the cellular level anti-proliferation effect, the compound can be used for preparing medicines for treating tumors, autoimmune diseases, inflammation or Alzheimer's disease, and has a wide development prospect.