76 results about "Chymotrypsin" patented technology

The invention relates to the technical field of biological extraction, and provides a cattle spleen peptide composition as well as a preparation method and application thereof. The preparation method of the cattle spleen peptide comprises the following steps: S1, taking a cattle spleen, adding normal saline, and homogenizing to obtain first homogenate; s2, adding combined enzyme into the first homogenate, uniformly stirring, and performing first water bath treatment to obtain second homogenate; s3, adding chymotrypsin into the second homogenate, uniformly stirring, and performing second water bath treatment to obtain third homogenate; s4, centrifuging the third homogenate to obtain supernate; s5, performing ultrafiltration and drying on the supernate to obtain the cattle spleen peptide. The combined enzyme comprises subtilisin, bromelain and papain. Through the technical scheme, the problem of low cattle spleen peptide extraction rate in related technologies is solved.

The invention belongs to the technical field of marine biological medicines, and relates to a hippocampus polypeptide for improving cognitive impairment of Alzheimer's disease and a preparation method and application thereof.The preparation method comprises the steps that hippocampus powder is dissolved in water, the pH value is adjusted to 1.8-2.5, pepsin is added for oscillation incubation for 2 hours, and then high-temperature heating is conducted to inactivate enzyme; adjusting the pH value to 7.3-8.0, adding chymotrypsin to form a compound enzyme enzymolysis combination, oscillating and incubating for 2 hours, and heating at high temperature to inactivate the enzyme; centrifuging the solution, collecting enzymatic hydrolysate, and filtering the obtained hippocampus crude polypeptide through an ultrafiltration membrane to obtain molecular weight lt; the hippocampus polypeptide is subjected to vacuum freeze drying and preservation. The hippocampus polypeptide extracted and prepared from hippocampus japonicus by adopting a biological enzymolysis method has excellent APP inhibitory activity, can remarkably improve dysmnesia, and can be used for developing related functional foods or medicines for treating Alzheimer's disease.

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention discloses a method for preparing high-F-value oligopeptide from whey protein, and belongs to the technical field of food biology. According to the method, whey protein is taken as a raw material, step-by-step synergistic enzymolysis of alkaline protease-chymotrypsin-flavourzyme is performed, and an activated carbon selective adsorption dearomatization process is combined, so that the F value and the hydrolysis degree of oligopeptide are remarkably improved. The enzymolysis efficiency is high, and the final hydrolysis degree is gt; 30%; the dearomatization effect is excellent, and the product F value is gt; 26; the method is simple in process and low in cost, high-value conversion of whey protein can be achieved, and the prepared high-F-value oligopeptide can be applied to the fields of sports nourishment, food for special medical purposes and the like and has remarkable industrial value.

The invention relates to the technical field of food, in particular to a preparation method of low-sensitization hydrolyzed whey protein nanoscale microcapsule powder, which comprises the following steps: S1, preparing a whey protein solution, performing microfiltration, and treating with cysteine hydrochloride; s2, trypsin hydrolysis; s3, inactivating and cooling; s4, adding chymotrypsin, and synchronously applying ultrasonic waves; s5, bromelain is added, ultrafiltration is performed, and peptide fragments are collected; s6, preparing a capsule wall solution; s7, adopting a micro-fluidic chip to form a nano emulsion; s8, the mixture is added into a CaCl2 solution to be stirred, multi-layer self-assembly is conducted, and micro-capsule particles are obtained; and S9, carrying out ultrahigh-pressure micro-jet homogenization, vacuum freeze drying and low-temperature plasma assisted grinding on the micro-capsule particles to obtain the nano-scale micro-capsule powder. The stability of active ingredients is maintained through multi-enzyme cooperation and ultrasonic-assisted efficient elimination of allergens, multi-layer nano-embedding under microfluidic control and low-temperature plasma grinding, and the limitations of low single enzymolysis efficiency, insufficient embedding rate and thermal denaturation risk in the prior art are overcome.

System (100) for the production of biomimetic magnetic nanocomposites for the immobilization of proteolytic enzymes and the targeted treatment of bovine uterine infections, comprising: a) a unit for producing a carrier matrix (102) configured for the production of magnetic nanoparticles; b) an enzyme preparation unit (104) configured to provide proteolytic enzymes selected from the group consisting of protease, trypsin, chymotrypsin, and papain; c) an immobilization unit (106) configured to immobilize the proteolytic enzymes on the modified renewable carrier matrix; and d) a characterization unit (108) configured to perform toxicological tests, comprising: - Equipment for testing antimicrobial activity, and - DPPH assay analysis system.

The present invention relates to a pharmaceutical composition for treating peptic gastric ulcer and a preparation method thereof, belonging to the field of biotechnology. The pharmaceutical composition contains chicken gizzard-membrane peptide, pumpkin seed extract, complex bacteria, white hyacinth bean flower extract and bletilla striata extract; the chicken gizzard-membrane peptide contains the products obtained by successively subjecting chicken gizzard-membrane to fractional enzymolysis with pepsin, chymotrypsin and keratinase; the pumpkin seed extract contains the products obtained by subjecting pumpkin seeds to co-enzymolysis with cellulase and pectinase and then successively to fractional enzymolysis with pepsin, nattokinase and aminopeptidase; the complex bacteria contain the co-fermentation bacteria of Lactobacillus acidophilus, Bifidobacterium and Lactobacillus casei; the white hyacinth bean flower extract is an ethanol percolation extract; the bletilla striata extract contains the products obtained by subjecting bletilla striata tubers to co-enzymolysis with cellulase and pectinase and then successively to fractional enzymolysis with pepsin, bromelain and serrapeptase. The various components are used in combination, having a good therapeutic effect on gastric ulcer, quickly and efficiently repairing gastric mucosa, and being safe and free of side effects.

The invention discloses a preparation method and application of enzymolysis royal jelly capable of whitening skin, fading spots and resisting wrinkles, and belongs to the technical field of enzymolysis of royal jelly. The technical problem to be solved is that the existing royal jelly zymolyte is lack of research having the effects of whitening, fading spots and resisting wrinkles. The key point of the technical scheme is that the preparation method of the enzymolysis royal jelly comprises the following steps: (1) mixing and homogenizing royal jelly and water, and adjusting the pH value to obtain a royal jelly solution; (2) adding compound protease into the royal jelly solution for enzymolysis, and performing enzyme deactivation and solid-liquid separation to obtain royal jelly enzymatic hydrolysate; the compound protease in the step (2) is fig protease, chymotrypsin and flavourzyme.

The application discloses a kind of low-toxicity broad-spectrum antibacterial peptide with WW as symmetry center, the antibacterial peptide is that WK is repeated twice and symmetrically distributed in two sides, and WW is used as symmetry center, obtain antibacterial peptide WKWKWWKWKW-NH2 (N2W2), then the tryptophan of C terminal and N terminal of N2W2 is replaced by leucine and / or isoleucine;The antibacterial peptide can basically maintain the excellent antibacterial activity of parent peptide, and their hemolytic activity is greatly reduced, and the safety is improved. The antibacterial activity of IL-N2W2 is highest, but the stability needs to be improved, so D-type amino acid replacement is carried out on IL-N2W2 to obtain D-IL-N2W2. D-IL-N2W2 maintains the high antibacterial activity and low toxicity of IL-N2W2, and can still completely inhibit bacterial growth after incubation with high-concentration trypsin or chymotrypsin, has very great potential in relieving antibiotic resistance, and has good application prospect in preparing clinical antibacterial drugs.

The invention discloses a chymotrypsin compound preparation for intervertebral disc degeneration through ultrasonic injection and a preparation method, and relates to the technical field of chymotrypsin compound preparation preparation. Comprising freeze-dried recombinant chymotrypsin powder, biodegradable nanoparticles loaded with chymotrypsin and a matrix synthesis promoting factor, and a microenvironment response type hydrogel precursor solution, the freeze-dried recombinant chymotrypsin powder comprises chymotrypsin, trehalose, mannitol and a nonionic surfactant, wherein the mass ratio of the chymotrypsin to the trehalose to the mannitol to the nonionic surfactant is 1: 10: 5: 1; freeze-dried chymotrypsin powder, ultrasonic response type PLGA nano-microspheres loaded with chymotrypsin and matrix synthesis promoting factors and a microenvironment response hydrogel precursor solution containing a sound-sensitive cross-linking agent are organically combined, and removal-regeneration synergistic treatment is achieved after percutaneous injection is conducted to a degenerated intervertebral disc.

The invention provides a C3-2 strain for producing bactrian camel chymosin and application of the C3-2 strain, and belongs to the technical field of biological engineering. According to the present invention, the Latin of the C3-2 strain is Kluyveromyces lactis, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251316. According to the method, the C3-2 strain is used for fermentation, the fermentation liquor is concentrated by adopting an ultrafiltration method, small molecular impurities are removed, and the bactrian camel chymosin with high enzyme activity is obtained by combining affinity chromatography. The cheese prepared from the bactrian camel chymosin obtained by the preparation method disclosed by the invention is high in crosslinking degree, harder and finer in texture, smaller, more uniform and more compact in microstructure texture, lower in bitter taste and higher in overall volatile flavor substance content, and the overall evaluation is superior to that of cheese prepared from commercial chymosin.

Provided herein are methods of making a peptide aptamer library of partially random peptide aptamers. The peptide aptamer library can be designed with tryptic and chymotryptic sites spaced to create domains. In each domain, a defined subset of amino acids are randomly ordered alongside invariant amino acids. Also provided herein are methods to identify peptide aptamers that bind a target using the peptide aptamer library disclosed herein.

The invention discloses a step-by-step enzymolysis method for alpha-lactalbumin allergic epitopes and application, and belongs to the technical field of dairy product processing. Through step-by-step directional enzymolysis of flavourzyme and alpha-chymotrypsin, allergic epitopes in ALA are directionally cleared, and the antigenicity of ALA is remarkably reduced. The method is simple and convenient in process, mild in condition and suitable for large-scale production, and the obtained enzymolysis product can be used for low-sensitization infant formula milk powder and food for special medical purposes and has important application value and social benefits.

The application provides a preparation method of oxygen-releasing gelatin for wound healing and application thereof, and relates to the technical field of gelatin preparation.The preparation method comprises the following steps: mixing gelatin with water, adding a mixture of neutral protease and chymotrypsin, carrying out enzymolysis at pH 5.5-6.5 and a temperature of 45-55 DEG C for 10-15 min, inactivating, and obtaining pretreated gelatin by using a dialysis bag with a molecular weight cut-off of 10-15 kDa; mixing the pretreated gelatin with water, adding 30wt%-32wt% hydrogen peroxide solution, stirring, adding 32wt%-34wt% hydrogen peroxide solution, and stirring; continuously adding 30wt%-32wt% hydrogen peroxide solution and stirring; obtaining a crude product; and purifying.The application combines specific enzymolysis pretreatment, an oxidation process and purification to prepare gelatin with better oxygen-releasing capacity, bacteriostatic capacity and significant promotion of skin wound healing, and the gelatin is safe and effective.

The application provides a polypeptide with angiotensin converting enzyme inhibitory activity and a preparation method and application thereof, and relates to the technical field of active peptide preparation. The abalone is pulverized, a complex enzyme is added for primary enzymolysis, and then a specific protease mixture containing prolyl endopeptidase, chymotrypsin and protease K is added for two-step enzymolysis, so that the obtained polypeptide solution has high ACE inhibitory activity. The obtained polypeptide is analyzed and screened to obtain polypeptides with amino acid sequences as shown in SEQ ID NO. 1-5. The polypeptide has high ACE inhibitory activity, and the IC 50 0.05mg / mL-0.40mg / mL, and therefore can play a blood pressure lowering effect as an angiotensin converting enzyme inhibitor.

The invention provides a marine bioactive protein peptide and application thereof in fresh keeping of chicken. The application of the marine bioactive protein peptide in fresh keeping of chicken comprises the following steps: S1, cutting chicken breast into blocks; s2, performing enzymolysis on the shrimp meat with bromelain and chymotrypsin, performing centrifugation, and adding lactic acid into supernate to prepare marine bioactive protein peptide; s3, adding the marine bioactive protein peptide into the hydroxypropyl methyl cellulose solution to prepare a fresh-keeping solution loaded with the marine bioactive protein peptide; and S4, soaking the blocky chicken breast in a fresh-keeping solution, taking out, draining, performing vacuum packaging, and performing cold storage. The fresh-keeping liquid loaded with marine bioactive protein peptides is used for keeping the chicken breast fresh, the oxidation process of chicken is delayed, water loss is remarkably reduced, and the sensory quality of chicken is kept.

The invention belongs to the technical field of comprehensive utilization of oyster mushrooms, and provides an oyster mushroom active peptide for inhibiting foaming of macrophages and a preparation method of the oyster mushroom active peptide. The method comprises the following steps: taking oyster mushroom as a raw material, preparing oyster mushroom protein through alkali extraction and acid precipitation, preparing a crude product of the oyster mushroom active peptide for inhibiting foaming of macrophages through compound enzymatic hydrolysis of the oyster mushroom protein by pepsin, trypsin and chymotrypsin, and separating and purifying the crude product by adopting ultrafiltration membrane ultrafiltration and Sephadex G-15 gel column chromatography to obtain the peptide with good activity and high purity. The bioactive peptide can reduce lipid accumulation, ROS level, TNF-alpha content and IL-6 content in RAW264.7 foamed macrophages, and effectively improve lipid accumulation, oxidative stress and inflammatory state of the foamed macrophages, thereby inhibiting foaming of the macrophages.

The present invention belongs to the field of polypeptide drugs, and specifically relates to a glycosylated bicyclic stapled peptide that can improve the anti-drug-resistant bacteria activity, improve the serum stability, and has good enzymatic hydrolysis stability on the basis of a template polypeptide, and its application. The present invention modifies the amino acid sequence of the template polypeptide SLP-0: Ac-LKRVWKRVFKLLKRYWRQLKKPVR-NH2 to obtain a glycosylated bicyclic stapled peptide. Compared with the template polypeptide SLP-0, its antibacterial effect is significantly improved, especially the antibacterial performance against Klebsiella pneumoniae (KP) is increased by 31 times compared with the template peptide SLP-0. Among them, SLP-51 has significant stability against trypsin, chymotrypsin and serum.

The invention provides a kidney-tonifying and anti-fatigue polypeptide composition and a preparation method thereof, and belongs to the technical field of medicinal biological peptide complexes, the composition comprises oyster polypeptide, stichopus japonicus polypeptide, a blue ear grass-dilleniad fruit composite extract, a cistanche-dendrobium officinale composite extract, a ginseng extract and a penis et testis cervi extract; the oyster polypeptide is a common enzymolysis product of oyster meat through trypsin and bovine chymotrypsin; the stichopus japonicus polypeptide is a common enzyme product of enzymolysis of stichopus japonicus body walls by alkaline protease and collagenase type II and subtilisin; the blue ear grass-dilleniad fruit composite extract is a product obtained by carrying out enzymolysis on blue ear grass and dilleniad fruit, extracting with an ethanol water solution and purifying with an SA sulfonic group silica gel strong cation exchange column; the cistanche-dendrobium officinale composite extract is a product obtained by carrying out enzymolysis on cistanche and dendrobium officinale, extracting with an ethanol water solution and purifying with a phenylboronic acid affinity column-polyamide column tandem column; and the synergistic effect of tonifying kidney and resisting fatigue is realized.

The invention provides a polypeptide with angiotensin converting enzyme inhibition as well as a preparation method and application thereof, and relates to the technical field of active peptide preparation. According to the method, the abalone is crushed, the compound enzyme is added for primary enzymolysis, then the specific protease mixture containing prolyl endopeptidase, chymotrypsin and protease K is added for secondary enzymolysis, and the obtained polypeptide liquid has high ACE (angiotensin converting enzyme) inhibition. The obtained polypeptide is analyzed and screened to obtain the polypeptide with the amino acid sequence as shown in SEQ ID NO.1-5. The polypeptide disclosed by the invention has high ACE (angiotensin converting enzyme) inhibition, and IC50 (50% of IC50) of the polypeptide to ACE is 0.05 mg / mL-0. 40mg / mL, so that the polypeptide can be used as an angiotensin converting enzyme inhibitor to play a role in reducing blood pressure.

The invention discloses a micro-ecological protective oral cleaning composition for whole-course control of dental calculus and application of the micro-ecological protective oral cleaning composition, and belongs to the technical field of oral care products. The composition contains citrate, a multienzyme mixture (at least two of lysozyme, xylanase and chymotrypsin) and a matrix additive. Citrate is chelated with calcium ions to soften and dissolve calcified dental calculus; enzymolysis of a multi-enzyme mixture inhibits early bacterial attachment and sediment accumulation and cooperates with citrate to decompose calcified dental calculus; the matrix additive ensures stable release of active ingredients. The components are synergistic, the whole process from the initial stage of dental calculus formation to calcification maturation intervenes, inhibits deposition and dissolves a calcification structure, and meanwhile, the composition can protect the micro-ecology of the oral cavity and has no obvious destructive effect on oral flora. The composition is applied to preparation of oral care products, can solve the problems of one-sided cleaning effect, poor hidden area effect and dysbacteriosis caused by sterilization of existing products, achieves the dual purposes of whole-course dental calculus control and micro-ecological maintenance, and is wide in application prospect.

The invention discloses a preparation method of cow milk exosomes, which comprises the following steps: 1) treating raw cow milk at 70-74 DEG C for 10-20 seconds to obtain a first liquid; (2) centrifuging the first liquid at 2500-3500 g for 20-40 minutes, and taking supernatant, so as to obtain second liquid; (3) adding chymotrypsin into the second liquid, incubating and centrifuging to obtain third liquid; 4) filtering the third liquid to obtain fourth liquid; 5) centrifuging the fourth liquid, and discarding the supernatant to obtain a first precipitate; 6) resuspending the first precipitate by using PBS, and centrifuging to obtain a second precipitate; and (7) resuspending the second precipitate by using PBS, performing ultrafiltration, and taking supernatant interception liquid, thereby obtaining the cow milk exosome. The exosome prepared by the invention is few in impure protein, good in anti-inflammatory activity and good in stability, and can be used for repairing intestinal barriers and inhibiting proinflammatory cytokines.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide CAMP502NC3 with high stability and application of the antibacterial peptide CAMP502NC3. The compound not only shows good antibacterial activity on staphylococcus aureus, but also can be applied to wide pH environments with pH of 2-8 and various protease environments, such as pepsin, trypsin and chymotrypsin; the compound can maintain a certain bactericidal ability under the conditions of high temperature and high salt concentration as high as 250 mM, shows high stability, and has the potential of preparing environment-stable antibacterial drugs.

The present invention provides a chymotrypsin detection reagent containing a compound represented by general formula (I) or a salt thereof. In the formula, R1 represents a substituent bonded to a benzene ring, p represents an integer of 0-4, R2, R3, R4, R5, R6, and R7 each independently represent a hydrogen atom or the like, R8 and R9 may each independently represent a hydrogen atom or the like, X represents a linear or branched C1-C3 alkylene group, R10 and R11 may each independently represent a hydrogen atom or the like, R12 may represent a hydrogen atom or the like, and L may represent a single bond or the like.

The present invention provides a polypeptide composition for oral administration, wherein the polypeptide composition comprises a polypeptide, an isolated BBI, an isolated KTI, fish oil, and at least one absorption promoter. The isolated BBI is mixed with the isolated KTI in a certain ratio, so that an activity ratio of total trypsin inhibitory (total TI) activity to total chymotrypsin inhibitory (total CI) activity in the composition is 0.5:1 to 10:1.

A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive / pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.

Provided herein are compositions, methods, and kits related to chymotrypsin production for characterizing proteins, assessing, and monitoring disease stages and phases, predicting the likelihood of disease progression, predicting and monitoring responses to disease therapies, and treating disease conditions.

The present invention relates to a green Trichoderma mutant strain with high beta-glucanase production, belonging to the field of microbial technology. The present invention provides a green Trichoderma (Trichoderma viride) UN-101 strain with high beta-glucanase production, with a deposit number of CGMCC NO.40414. The present invention also discloses an industrial production method for producing beta-glucanase using the strain, and the fermentation broth enzyme activity can reach more than 53000U / mL by seed culture and liquid deep fermentation with the strain, and the beta-glucanase product obtained by extraction has low finished product cost, high beta-glucanase enzyme activity, good stability and strong specificity. The invention is characterized in that the optimum reaction temperature is 45°C, and the enzyme activity can still be maintained at more than 85% when kept at 80°C for 2 hours, and the thermal stability is good; the optimum reaction pH is 3.0, and the enzyme can maintain more than 80% of its activity when treated in the pH range of 2.0-6.5 for 2 hours, and is an acidic glucanase; the invention has excellent resistance to hydrolysis by pepsin, trypsin and chymosin.

What is provided is a therapeutic agent that is effective for the treatment of ovarian clear cell carcinoma. A therapeutic agent for ovarian clear cell carcinoma includes, as an active ingredient, a proteasome inhibitor. Furthermore, in the therapeutic agent for ovarian clear cell carcinoma, the proteasome inhibitor is a substance that reversibly or irreversibly binds to a 20s proteasome-β5 subunit and inhibits a chymotrypsin-like activity. Moreover, in the therapeutic agent for ovarian clear cell carcinoma, the proteasome is a 26s proteasome. In addition, in the therapeutic agent for ovarian clear cell carcinoma, a content proportion of the proteasome inhibitor is 80% by mass or more, 90% by mass or more, or 100% by mass.