33 results about "Chymotrypsin" patented technology

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention discloses a preparation method and application of enzymolysis royal jelly capable of whitening skin, fading spots and resisting wrinkles, and belongs to the technical field of enzymolysis of royal jelly. The technical problem to be solved is that the existing royal jelly zymolyte is lack of research having the effects of whitening, fading spots and resisting wrinkles. The key point of the technical scheme is that the preparation method of the enzymolysis royal jelly comprises the following steps: (1) mixing and homogenizing royal jelly and water, and adjusting the pH value to obtain a royal jelly solution; (2) adding compound protease into the royal jelly solution for enzymolysis, and performing enzyme deactivation and solid-liquid separation to obtain royal jelly enzymatic hydrolysate; the compound protease in the step (2) is fig protease, chymotrypsin and flavourzyme.

The application discloses a kind of low-toxicity broad-spectrum antibacterial peptide with WW as symmetry center, the antibacterial peptide is that WK is repeated twice and symmetrically distributed in two sides, and WW is used as symmetry center, obtain antibacterial peptide WKWKWWKWKW-NH2 (N2W2), then the tryptophan of C terminal and N terminal of N2W2 is replaced by leucine and / or isoleucine;The antibacterial peptide can basically maintain the excellent antibacterial activity of parent peptide, and their hemolytic activity is greatly reduced, and the safety is improved. The antibacterial activity of IL-N2W2 is highest, but the stability needs to be improved, so D-type amino acid replacement is carried out on IL-N2W2 to obtain D-IL-N2W2. D-IL-N2W2 maintains the high antibacterial activity and low toxicity of IL-N2W2, and can still completely inhibit bacterial growth after incubation with high-concentration trypsin or chymotrypsin, has very great potential in relieving antibiotic resistance, and has good application prospect in preparing clinical antibacterial drugs.

The invention discloses a chymotrypsin compound preparation for intervertebral disc degeneration through ultrasonic injection and a preparation method, and relates to the technical field of chymotrypsin compound preparation preparation. Comprising freeze-dried recombinant chymotrypsin powder, biodegradable nanoparticles loaded with chymotrypsin and a matrix synthesis promoting factor, and a microenvironment response type hydrogel precursor solution, the freeze-dried recombinant chymotrypsin powder comprises chymotrypsin, trehalose, mannitol and a nonionic surfactant, wherein the mass ratio of the chymotrypsin to the trehalose to the mannitol to the nonionic surfactant is 1: 10: 5: 1; freeze-dried chymotrypsin powder, ultrasonic response type PLGA nano-microspheres loaded with chymotrypsin and matrix synthesis promoting factors and a microenvironment response hydrogel precursor solution containing a sound-sensitive cross-linking agent are organically combined, and removal-regeneration synergistic treatment is achieved after percutaneous injection is conducted to a degenerated intervertebral disc.

The invention provides a C3-2 strain for producing bactrian camel chymosin and application of the C3-2 strain, and belongs to the technical field of biological engineering. According to the present invention, the Latin of the C3-2 strain is Kluyveromyces lactis, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20251316. According to the method, the C3-2 strain is used for fermentation, the fermentation liquor is concentrated by adopting an ultrafiltration method, small molecular impurities are removed, and the bactrian camel chymosin with high enzyme activity is obtained by combining affinity chromatography. The cheese prepared from the bactrian camel chymosin obtained by the preparation method disclosed by the invention is high in crosslinking degree, harder and finer in texture, smaller, more uniform and more compact in microstructure texture, lower in bitter taste and higher in overall volatile flavor substance content, and the overall evaluation is superior to that of cheese prepared from commercial chymosin.

The invention discloses a step-by-step enzymolysis method for alpha-lactalbumin allergic epitopes and application, and belongs to the technical field of dairy product processing. Through step-by-step directional enzymolysis of flavourzyme and alpha-chymotrypsin, allergic epitopes in ALA are directionally cleared, and the antigenicity of ALA is remarkably reduced. The method is simple and convenient in process, mild in condition and suitable for large-scale production, and the obtained enzymolysis product can be used for low-sensitization infant formula milk powder and food for special medical purposes and has important application value and social benefits.

The application provides a preparation method of oxygen-releasing gelatin for wound healing and application thereof, and relates to the technical field of gelatin preparation.The preparation method comprises the following steps: mixing gelatin with water, adding a mixture of neutral protease and chymotrypsin, carrying out enzymolysis at pH 5.5-6.5 and a temperature of 45-55 DEG C for 10-15 min, inactivating, and obtaining pretreated gelatin by using a dialysis bag with a molecular weight cut-off of 10-15 kDa; mixing the pretreated gelatin with water, adding 30wt%-32wt% hydrogen peroxide solution, stirring, adding 32wt%-34wt% hydrogen peroxide solution, and stirring; continuously adding 30wt%-32wt% hydrogen peroxide solution and stirring; obtaining a crude product; and purifying.The application combines specific enzymolysis pretreatment, an oxidation process and purification to prepare gelatin with better oxygen-releasing capacity, bacteriostatic capacity and significant promotion of skin wound healing, and the gelatin is safe and effective.

The invention belongs to the technical field of comprehensive utilization of oyster mushrooms, and provides an oyster mushroom active peptide for inhibiting foaming of macrophages and a preparation method of the oyster mushroom active peptide. The method comprises the following steps: taking oyster mushroom as a raw material, preparing oyster mushroom protein through alkali extraction and acid precipitation, preparing a crude product of the oyster mushroom active peptide for inhibiting foaming of macrophages through compound enzymatic hydrolysis of the oyster mushroom protein by pepsin, trypsin and chymotrypsin, and separating and purifying the crude product by adopting ultrafiltration membrane ultrafiltration and Sephadex G-15 gel column chromatography to obtain the peptide with good activity and high purity. The bioactive peptide can reduce lipid accumulation, ROS level, TNF-alpha content and IL-6 content in RAW264.7 foamed macrophages, and effectively improve lipid accumulation, oxidative stress and inflammatory state of the foamed macrophages, thereby inhibiting foaming of the macrophages.

The invention discloses a micro-ecological protective oral cleaning composition for whole-course control of dental calculus and application of the micro-ecological protective oral cleaning composition, and belongs to the technical field of oral care products. The composition contains citrate, a multienzyme mixture (at least two of lysozyme, xylanase and chymotrypsin) and a matrix additive. Citrate is chelated with calcium ions to soften and dissolve calcified dental calculus; enzymolysis of a multi-enzyme mixture inhibits early bacterial attachment and sediment accumulation and cooperates with citrate to decompose calcified dental calculus; the matrix additive ensures stable release of active ingredients. The components are synergistic, the whole process from the initial stage of dental calculus formation to calcification maturation intervenes, inhibits deposition and dissolves a calcification structure, and meanwhile, the composition can protect the micro-ecology of the oral cavity and has no obvious destructive effect on oral flora. The composition is applied to preparation of oral care products, can solve the problems of one-sided cleaning effect, poor hidden area effect and dysbacteriosis caused by sterilization of existing products, achieves the dual purposes of whole-course dental calculus control and micro-ecological maintenance, and is wide in application prospect.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide CAMP502NC3 with high stability and application of the antibacterial peptide CAMP502NC3. The compound not only shows good antibacterial activity on staphylococcus aureus, but also can be applied to wide pH environments with pH of 2-8 and various protease environments, such as pepsin, trypsin and chymotrypsin; the compound can maintain a certain bactericidal ability under the conditions of high temperature and high salt concentration as high as 250 mM, shows high stability, and has the potential of preparing environment-stable antibacterial drugs.

Provided herein are compositions, methods, and kits related to chymotrypsin production for characterizing proteins, assessing, and monitoring disease stages and phases, predicting the likelihood of disease progression, predicting and monitoring responses to disease therapies, and treating disease conditions.

The application provides a kidney-tonifying and anti-fatigue polypeptide composition and a preparation method thereof, belongs to the technical field of medicinal biological peptide complexes, and the composition comprises oyster polypeptide, holothurian polypeptide, spider silk and blue ears grass-pentamerous fruit composite extract, cistanche-dendrobium officinale composite extract, ginseng extract and deer penis extract; the oyster polypeptide is a common enzymolysis product of oyster meat by trypsin and bovine chymotrypsin; the holothurian polypeptide is a common enzymolysis product of holothurian body wall by alkaline protease, collagenase type II and subtilisin; the spider silk and blue ears grass-pentamerous fruit composite extract is a product obtained by the following steps: subjecting spider silk and blue ears grass and pentamerous fruit to enzymolysis, extracting with an ethanol aqueous solution, and purifying with a SA sulfonic acid silica gel strong cation exchange column; the cistanche-dendrobium officinale composite extract is a product obtained by the following steps: subjecting cistanche and dendrobium officinale to enzymolysis, extracting with an ethanol aqueous solution, and purifying with a phenylboronic acid affinity column-polyamide column series connection column; and the composition realizes the synergistic effect of kidney tonification and anti-fatigue.

The invention belongs to the technical field of biology, and particularly relates to separation and application of proteasome-rich human placenta-derived vascular stem cell outer vesicles. The extracellular vesicles provided by the invention are derived from human placenta source blood vessel promoting stem cells, and the human placenta source blood vessel promoting stem cells show that markers PAI-1 are positive, MECOM is positive, CD201 is positive, CD44 is positive, CD73 is positive, HLA-ABC is positive, CD34 is negative, CD31 is negative and CD326 is negative. Compared with human umbilical cord mesenchymal stem cell outer vesicles, the human placenta-derived vascular stem cell outer vesicles provided by the invention are rich in proteasomes and angiogenic protein factors, wherein the proteasomes have high chymotrypsin-like activity. Moreover, in-vivo and in-vitro experimental data show that the human placenta-derived vascular stem cell outer vesicles provided by the invention are rich in proteasomes, and can degrade accumulation of HIF and inhibit cell apoptosis; the medicine has a treatment effect on myocardial infarction.

The invention belongs to the technical field of food, and particularly discloses hydrolyzed whey powder with sheep flavor and a preparation method thereof, and the preparation method comprises the following steps: adding sheep whey protein into water, and dissolving to obtain protein liquid; adding flavourzyme, chymotrypsin and alkaline protease into the protein liquid, and performing enzymolysis and enzyme deactivation to obtain enzymatic hydrolysate; and carrying out nanofiltration on the enzymatic hydrolysate by using a nanofiltration membrane with the molecular weight cut-off of 150Da, and carrying out spray drying to obtain the hydrolyzed whey powder. The preparation method comprises the following steps: dissolving sheep whey protein in water, carrying out enzymolysis by using flavourzyme, chymotrypsin and alkaline protease, carrying out ultrafiltration by using ultrafiltration membranes with different molecular weights, and carrying out spray drying to obtain the hydrolyzed whey powder with sheep flavor, the content of beta-lactoglobules in the hydrolyzed whey powder is reduced by more than 90% compared with that before hydrolysis, and the content of beta-lactoglobules in the hydrolyzed whey powder is reduced by more than 90% compared with that before hydrolysis. The proportion of small molecules with the molecular weight smaller than 1000 Da in total protein is larger than 60%, bitterness is avoided, and wide application prospects are achieved.

The present application relates to the technical field of antibacterial peptide application, and particularly relates to an antibacterial peptide CAMP502NC3 with high stability and application thereof.The antibacterial peptide CAMP502NC3 not only exhibits good antibacterial activity on staphylococcus aureus, but also can maintain certain bactericidal capacity in a wide pH environment of pH 2-8, a variety of protease environments, for example, pepsin, trypsin, chymotrypsin, and a salt concentration condition of up to 250 mM, and shows high stability and has the potential to prepare environment-stable antibacterial drugs.

The present application belongs to the field of bioactive peptides. The present application provides an angiotensin converting enzyme inhibitory peptide and a screening method and application thereof, and the amino acid sequence of the inhibitory peptide is GKGLW and / or GDGLKW. The screening method of the inhibitory peptide of the present application uses soybean protein as raw material, adopts targeted alkaline protease and chymotrypsin double enzymolysis technology for preparation and screening, realizes rapid screening of ACE inhibitory peptides, and provides experimental basis for structure-activity relationship. The inhibitory peptide has strong blood pressure lowering activity, high safety and no toxic side effects. The determination of the stability of the digestive tract shows that the inhibitory peptide has good gastrointestinal stability in the gastrointestinal digestion, and can still maintain good activity after entering the blood.

A method for increasing peptide fragmentation by labelling the peptide at the C-terminal end with a guanidinium group or other basic functional group and distinguishing isobaric amino acids and amino acid combinations of asparagine and glycine-glycine; glutamine and glycine-alanine; and / or glutamine and alanine-glycine, during polypeptide sequencing. The method involves: obtaining a peptide of interest and / or digesting a polypeptide of interest with a protease, such as pepsin, chymotrypsin or trypsin, or by chemical cleavage to produce shorter peptides; reacting the obtained and / or generated peptides with a coupling reagent to derivatize the free C-terminal carboxylic acid function of the peptides, thus adding a basic functional group rendering C-terminal peptide fragment ions detectable by mass spectrometry; selecting a charge state of 2+ or more, and fragmenting the derivatized peptides in a mass spectrometer under conditions effective to generate at least w ions; and detecting the w ions by mass spectrometry, and identifying derivatized peptides which incorporate the additional mass of the basic functional group.

The application discloses a kind of central symmetry β-hairpin antibacterial peptide with KW repeat sequence with R n As corner, and application thereof, the antibacterial peptide is with R, RR or RRR as corner, WK repeat unit is alternately arranged in two sides, its structure general formula is (WK) x R n (KW) x -NH2, marked as R n W x ;Wherein, n=1,2,3;X=2,3.In vitro antibacterial experiment and hemolytic activity experiment show that this kind of antibacterial peptide has strong antibacterial activity and almost negligible hemolytic toxicity.After D-type amino acid full replacement of R2W3 sequence, the antibacterial activity of D-R2W3 is further enhanced, the hemolytic toxicity is low, and it can resist the hydrolysis of trypsin and chymotrypsin, has very great potential in relieving antibiotic resistance, and has good application prospect in preparing clinical antibacterial drugs.

The invention belongs to the technical field of experimental model preparation, and provides a construction method of an in-vitro articular cartilage superficial layer injury model aiming at proteoglycan and collagenous fibers in a superficial cartilage layer (SFZ) in order to simulate cartilage surface component loss and structural damage in the early stage of OA morbidity, and the in-vitro articular cartilage superficial layer injury model is constructed by taking a pig knee joint cartilage as a material. A mixed solution of 0.01 mg / ml chymotrypsin and 0.5 mg / ml collagenase D is used as digestive enzyme, the surface of an in-vitro articular cartilage column is treated for 20 min, proteoglycan and collagenous fibers in SFZ are accurately destroyed, and an in-vitro articular cartilage superficial layer damage model is constructed; histological staining, microscopic observation and mechanical detection results all show that the method can cause SFZ structure damage and mechanical property reduction on the premise of not influencing deep tissues. The model has the advantages of simplicity and convenience in operation, low cost and controllable injury range, and provides a reliable experimental tool for research on early pathological mechanisms of osteoarthritis and exploration of related diagnosis and treatment means.

The invention discloses a preparation method and application of antioxidant salmon protein oligopeptide, and belongs to the technical field of aquatic product processing. The method comprises the following steps: respectively extracting salmon skin crude protein fluid, salmon swimming bladder crude protein fluid and salmon meat crude protein fluid; the three components are mixed according to the mass ratio of (75-115): (35-55): (65-85), and ascorbyl palmitate, flavourzyme and chymotrypsin are added for synergistic enzymolysis; and separating the enzymatic hydrolysate by using a 1800 Da ultrafiltration membrane, and drying to obtain a target product. Through synergistic utilization of three salmon by-products and integrated innovation of a quaternary enzymolysis system and a three-section type activity protection network, the prepared salmon protein oligopeptide has the molecular weight of less than 1800 Da, the polypeptide purity of more than or equal to 92% and the proportion of 500-1000 Da small molecule peptide of more than or equal to 64%, and shows excellent free radical scavenging capacity (the DPPH scavenging rate is more than or equal to 88%, and the ABTS scavenging rate is more than or equal to 92%). The product can be widely applied to the fields of functional foods, health foods and cosmetics.

The application discloses a chymotrypsin near-infrared fluorescent probe with a large stokes shift and a preparation method and application thereof. A fluorescent probe molecule TCF-CHT has a structural formula as shown in formula I. When a 530nm excitation wavelength is used for excitation, the TCF-CHT itself has weak fluorescence at 670nm, but it can selectively have a fluorescence opening reaction with the CHT. With the increase of the CHT concentration, the 670nm fluorescence intensity is gradually enhanced, and other common interfering ions and species cannot obviously interfere with and influence the TCF-CHT as an analysis reagent for detecting the CHT. Since the emission wavelength is in the near-infrared region and a large stokes shift (140nm) is generated, the interference of the system background fluorescence is greatly reduced, and the sensitivity of the CHT activity detection is improved. Therefore, the TCF-CHT is suitable for the fluorescence detection of the CHT with high selectivity and high sensitivity.

The present disclosure relates to compounds that exhibit activity in the inhibition of chymotrypsin-like proteases and pharmaceutical compositions comprising these compounds, and methods of treating viral infections by administering these compounds or pharmaceutical compositions.

The application belongs to the technical field of preparation of adsorption separation functional materials, and relates to a method for preparing a polyvinyl phosphonic acid grafted enzyme fixed magnetic nanochain adsorbent by droplet confinement, and high-efficiency uranium adsorption application of the adsorbent. The application can construct the effective uranium ion trap in the continuous water phase of the high internal phase emulsion through the space limited conjugation of the enzyme fixed magnetic nanochain and the polyvinyl phosphonic acid polymer. The polyvinyl phosphonic acid polymer is grown from the surface of a model enzyme, i.e. alpha-chymotrypsin, by using a novel water-soluble atom transfer radical polymerization (ATRP) initiator, and provides high-density accessible phosphonic groups for capturing uranium ions, so that the polyvinyl phosphonic acid polymer and the enzyme fixed magnetic nanochain adsorbent with uniform surface functional sites are prepared, and are applied to high-efficiency adsorption of uranium cation.

The present application relates to a kind of skin regeneration repair medicine and preparation method thereof, belong to biological medicine technical field, and medicine includes oil palm extraction peptide, tiger palm silver ear extract, Gangdeng root-milletia nitida-Siberian milkvetch composite extract and pomegranate peel polyphenol etc.Oil palm extraction peptide is oil palm sequentially by cellulase, alpha-chymotrypsin and ficin enzymolysis, then by G-50 dextran gel column purification and obtain the product above 1kDa;Tiger palm silver ear extract is tiger palm silver ear fruit body by cellulase and bromelain common enzymolysis, and obtain the product below 3kDa;Gangdeng root-milletia nitida-Siberian milkvetch composite extract is Gangdeng root, milletia nitida and siberian milkvetch by gradient extraction of ethanol aqueous solution and obtain extract, sequentially by polyamide resin column adsorption and LH-60 dextran gel column purification and obtain the product.Multiple components construct good repair system, accelerate wound repair healing.

A method for screening and preparing antioxidant peptides from mare's milk whey protein and uses thereof. Potential antioxidant polypeptides are screened based on in silico enzymolysis and computer-based prediction, followed by molecular docking with the keap1 protein. The feasibility of obtaining antioxidant peptides is then validated by simulating gastrointestinal digestion using pepsin, trypsin, and chymotrypsin. Subsequently, high-purity antioxidant peptides are chemically synthesized and subjected to in vitro chemical antioxidant activity assays.

The application provides a method for improving the stability and solubility of fish myofibrillar protein, and belongs to the technical field of aquatic protein processing. The method comprises the following steps: mixing fish myofibrillar protein solution with enzymes and carrying out enzymolysis; the enzymes include one or more of glutamine enzyme, protein glutamine enzyme, chymotrypsin and papain. The method provided by the application improves the stability and low-salt solubility of fish myofibrillar protein.

The invention discloses a traditional Chinese and western medicine combined medicine for treating tympanic membrane perforation and a preparation method thereof, the traditional Chinese and western medicine combined medicine comprises traditional Chinese medicine components and western medicine components, and the traditional Chinese medicine components comprise 6-30 g of safflower carthamus, 10-40 g of angelica sinensis, 6-30 g of frankincense, 6-30 g of myrrh, 6-30 g of ligusticum wallichii and 10-20 g of pollen typhae; the western medicine component is alpha-chymotrypsin, and the dosage of the alpha-chymotrypsin is 4000U per milliliter of the mixed solution. The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese and western medicine combined medicine for treating tympanic membrane perforation and a preparation method thereof.

Provided herein are compositions, methods, and kits related to chymotrypsin production for characterizing proteins, assessing, and monitoring disease stages and phases, predicting the likelihood of disease progression, predicting and monitoring responses to disease therapies, and treating disease conditions.

The invention provides a marine bioactive polypeptide as well as preparation and application thereof, and relates to the technical field of bioactive peptide preparation. According to the method, the abalone is crushed, the compound enzyme is added for primary enzymolysis, then the specific protease mixture containing prolyl endopeptidase, chymotrypsin and protease K is added for secondary enzymolysis, and the obtained polypeptide liquid has high ACE (angiotensin converting enzyme) inhibition. The obtained polypeptide is analyzed and screened to obtain the polypeptide with the amino acid sequence as shown in SEQ ID NO.1-5. The polypeptide disclosed by the invention has high ACE (angiotensin converting enzyme) inhibition, and IC50 (50% of IC50) of the polypeptide to ACE is 0.05 mg / mL-0. 40mg / mL, so that the polypeptide can be used as an angiotensin converting enzyme inhibitor to play a role in reducing blood pressure.