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Bacteroides signal molecule microecological preparation and its preparation method

A technology of microecological preparations and signal molecules, applied in the field of pharmaceutical products derived from bacteria, can solve problems such as unfavorable quality control, difficult quality control, difficult quantification of dead bacteria, etc., to achieve high bacterial mortality, regulation of immune function, The effect of regulating blood lipid metabolism

Inactive Publication Date: 2008-02-20
SENBAIAO SCI & TECH UNIV DALIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the second-generation product avoids the two bottlenecks of the number of viable bacteria and the survival period, it also has fatal shortcomings
This is that the dead bacteria are difficult to quantify, which is not conducive to quality control, and it is actually difficult to become a qualified product
The second-generation products appeared relatively late, and only a few domestic enterprises applied for approval of the second-generation products. Because the quality is not easy to control, the national examination and approval authority is still under consideration.
[0016] In summary, although the experimental research and theoretical discussion on the transmission of various physiological regulatory information between the signal molecules contained in the normal intestinal flora and the somatic cells have made great progress and achieved effective results, there is still no possibility Emergence of effective products that provide practical applications

Method used

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  • Bacteroides signal molecule microecological preparation and its preparation method
  • Bacteroides signal molecule microecological preparation and its preparation method
  • Bacteroides signal molecule microecological preparation and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1. The preparation process of Bacteroides signal molecule products is as follows:

[0037] Insert the sterilized Bacteroides fragilis DM9808 (B.flagili, subsp.Thitaiotamicron) bacterial strain seed solution into the content by weight ratio: peptone 1.5-2%, soybean peptone 0.5%, glucose 0.5%, disodium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.1%, beef extract 0.2%, yeast extract 1%, sodium chloride 0.3%, soluble starch 0.5%. After the culture liquid base undergoes anaerobic fermentation at 35-38°C, it is centrifuged and concentrated to obtain the Bacteroides cell concentrate. Add physiological saline to the Bacteroides thallus concentrate, and break the bacterium cell wall by high pressure. Add trypsin (1-2mg / ml), chymotrypsin (1-2mg / ml), pepsin (1-2mg / ml) and hydrochloric acid (to make the concentration 0.01M / l), hydrolyze for 12-24 hours, Centrifuge and wash with water to obtain crude cell wall extract. Freeze dry this product. The obtained ...

Embodiment 2

[0039] Embodiment two, insert the Bacteroides fragilis common subspecies DM9707 (B.flagili, subsp.vulgatus) bacterial strain seed liquid into the content weight ratio after sterilization: peptone 1.5~2%, soybean peptone 0.5%, glucose 0.5%, disodium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.1%, beef extract 0.2%, yeast extract 1%, sodium chloride 0.3%, soluble starch 0.5%. After the culture liquid base undergoes anaerobic fermentation at 35-38°C, it is centrifuged and concentrated to obtain the Bacteroides cell concentrate. Add physiological saline to the Bacteroides thallus concentrate, and break the bacterium cell wall by high pressure. Add trypsin (1-2mg / ml), chymotrypsin (1-2mg / ml), pepsin (1-2mg / ml) and hydrochloric acid (to make the concentration 0.01M / l), hydrolyze for 12-24 hours, Centrifuge and wash with water to obtain crude cell wall extract. Freeze dry this product. The obtained dried product is finely ground in a mortar and sieved to obtain the o...

Embodiment 3

[0041] Example 3: Insert the seed solution of B.flagilis melannogenicus bacterial strain into the sterilized solution containing: peptone 1.5-2%, soybean peptone 0.5%, glucose 0.5%, disodium hydrogen phosphate 0.4 %, potassium dihydrogen phosphate 0.1%, beef extract 0.2%, yeast extract 1%, sodium chloride 0.3%, soluble starch 0.5%. After the culture liquid base undergoes anaerobic fermentation at 35-38°C, it is centrifuged and concentrated to obtain the Bacteroides cell concentrate. Add physiological saline to the Bacteroides thallus concentrate, and break the bacterium cell wall by high pressure. Add trypsin (1-2mg / ml), chymotrypsin (1-2mg / ml, pepsin (1-2mg / ml) and hydrochloric acid (to make the concentration 0.01M / l) respectively, hydrolyze for 12-24 hours, centrifuge and washing with water to obtain the crude cell wall extract. The product is freeze-dried. The obtained dry matter is ground in a mortar and sieved to obtain the original powder of the Bacteroides molecular mi...

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Abstract

The present invention relates to a b.fragilis signal molecule microbial preparation, the b.fragilis bacterium are inoculated in a culture medium for anaerobic fermentation at 35 to 38 DEG C, after that, the b.fragilis bacteria concentrate is obtained by centrifugal concentration; the bacteria cell walls are broken by high pressure; further, the bacteria concentrate is treated with enzyme hydrolysis by trypsin, chymotrypsin and pepsin, and then the preparation raw powder is obtained by solvent extraction and decompression drying. The preparation raw powder is taken as the main ingredient, and other excipients are added to prepare the oral capsules, tablets, granules, oral liquor and other preparations. The b.fragilis signal molecule product of the present invention is proven to have no acute toxicity, no influence on the times of spontaneous activity, blood pressure and respiration of the animals and can not cause the allergic reactions of the animals by animal trials. The animal trials further prove that: the product has the effects of regulating blood-lipid metabolism, reducing fat accumulation and slimming; at the same time, the present invention can improve the immunity and the anti-tumor effect by adjusting the gene expression and regulating the immune functions.

Description

technical field [0001] The present invention relates to the field of pharmaceutical preparations derived from microbial materials, in particular those derived from bacteria. Background technique [0002] The production technology of probiotics products (Probiotics) has gone through the development of the first generation product and the second generation product. The first generation products are live bacteria products, and the second generation products are dead bacteria products. These two eras have passed through the course of half a century. The first-generation products mainly use live bacteria of Bifidobacterium and Lactic acid bacteria. Its mechanism of action is to adjust intestinal flora imbalance (Dysbacteriosis), supplement beneficial bacteria, inhibit harmful bacteria and maintain micro-ecological balance (Eubiosis), which is beneficial to human health. As a micro-ecological regulator, live probiotic products have been widely used in the world as an effective ...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K9/16A61K9/20A61P37/02A61P35/00A61K35/741
Inventor 康白袁杰力所伟
Owner SENBAIAO SCI & TECH UNIV DALIAN
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