Method used for testing high throughput acute toxicity of early life stage of fish
A technology of acute toxicity and testing methods, which is applied in the direction of testing water, measuring devices, instruments, etc., can solve the problems of large individual test organisms, large individual differences, long test time, etc., and meet the requirements of reducing experimental costs and technical levels , the effect of acute toxicity
Inactive Publication Date: 2012-07-04
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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[0004] The purpose of the present invention is to provide a simple, economical, rapid and high-throughput method for overcoming the shortcomings of the traditional adult fish acute toxicity test, such as large individuals, small numbers, large individual differences, long test time, and low reproducibility. The acute toxicity test method provides technical support for the acute toxicity test of toxic chemical pollutants and environmental samples
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[0011] The collected Japanese medaka eggs are carefully selected, placed in the prepared recombined dilution solution, and placed in a constant temperature incubator at 40°C for cultivation and hatching. Collect the hatched larvae, continue to put them into the dilute solution and cultivate them for 6 hours, and use them as the test organisms. Put 5 ml of the solution of the pollutant to be tested in each well of a 6-well plate, put 5 tested larvae in each well, and then put them in a constant temperature incubator at 25°C for 12 hours, record the number of dead larvae, and calculate the pollution 50% fatality rate.
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The invention relates to a special method for testing the high throughput acute toxicity of an early life stage of fish, and belongs to the technical field of environment detection and assessment. According to the method, the sensibility of the fish at different life stages on toxic pollutants as well as the influence of conditions such as different quantities of test creatures, different exposed containers and the like on test results are discussed through developing a recombinant dilute solution for toxicity test, thus realizing high throughput on a larval fish acute toxicity test. The method is simple, convenient, economical, rapid and sensitive, has low requirements on experiment operation technical level, and is convenient for popularization and application.
Description
technical field [0001] The invention relates to a high-throughput acute toxicity test method specially aimed at early life stages of fish, belonging to the technical category of environmental detection and evaluation. Background technique [0002] With the rapid development of social economy and the intensification of industrial and agricultural production activities, more and more chemicals are produced and used. According to reports, the number of registered chemicals exceeds tens of millions. Daily life and production The number of chemicals used in more than 100,000 species. These chemicals may enter the environment or remain in the human body and organisms through various channels during the production and use process, posing a serious threat to the ecological environment and human health. In order to accurately evaluate the safety of chemicals and environmental samples, biological toxicity effects are essential and the most important basic data. [0003] There are do...
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IPC IPC(8): G01N33/18G01N33/00
Inventor 魏东斌谭卓威杜宇国
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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