246results about How to "Shorten experiment time" patented technology

The invention discloses a capture probe group for detecting pathogenic microorganisms and application of the capture probe group. The nucleic acid sequence of the capture probe group comprises a sequence as shown in SEQ ID NO. 1 to SEQ ID NO. 101. The capture probe group can cover and detect 119 types of bacteria, 32 types of fungi, 41 types of DNA viruses and 71 types of RNA viruses and covers common infectious disease pathogens, the kit containing the capture probe can cover and detect 88 types of drug-resistant and virulence genes, the detection performance of pathogenic microorganisms is greatly improved, and the sensitivity and specificity are improved.

The invention provides a residual stress prediction method considering ultrasonic peening process parameters of a welded joint, and the method specifically comprises the following steps: carrying out thermal-mechanical coupling simulation of a welded structure based on a finite element method, obtaining a residual stress field of the welded structure, taking the residual stress field as initial stress, and building an ultrasonic peening processing finite element model; on the basis of a hypercube Latin sampling method, a parameter combination sample is extracted, corresponding residual stress data after ultrasonic peening are obtained through simulation, and a residual stress prediction function model containing an undetermined coefficient is established with ultrasonic peening parameters as independent variables. And based on a particle swarm optimization algorithm, training to obtain an undetermined coefficient value which enables a prediction effect to be optimal, and completing establishment of a prediction function model. Therefore, the optimal process parameter combination of ultrasonic peening can be obtained so as to improve the mechanical property of a welded joint. According to the residual stress prediction method considering the ultrasonic impact process parameters of the welding joint, a basis is provided for improving the service performance of a large-scale thin-wall part welding structure.

The invention relates to a detection kit for epithelial-mesenchymal transition in EB virus infected thymoma and application, the detection kit comprises a quantum dot labeled antibody E-Cadherin and a quantum dot labeled antibody Vimentin, a quantum dot is coupled with a specific antibody, a thymoma paraffin section sample is dyed through the quantum dot and the specific antibody conjugate, and the EB virus infected thymoma is obtained. The conjugate can be specifically connected with an EMT related antigen expressed in the slice, so that the quantum dots can be used for qualitative or quantitative detection of the EMT-generated thymoma cells. The quantum dots are used for replacing fluorescent dye, direct marking of tissue slices can be achieved, local positioning of epithelial cells and mesenchymal tissues in the thymoma microenvironment is achieved, number detection and position detection can be achieved, and EMT condition evaluation is facilitated.

The invention provides an electric arc additive manufacturing weld bead morphology prediction method based on three-dimensional scanning point cloud. The method comprises the following steps that firstly, a weld bead of an electric arc additive manufacturing stainless steel weldment is obtained through an electric arc additive manufacturing stainless steel experiment; 2, a three-dimensional point cloud model of the stainless steel welding beads manufactured through the electric arc additive is obtained through a handheld three-dimensional scanner, and each welding bead is cut and straightened; thirdly, the straightened uniform arc additive manufacturing stainless steel weld bead is subjected to slice projection in the Y direction, and the average shape of the weld bead is obtained and serves as a data set; 4, determining input, output and the number of hidden neurons, and establishing a neural network model suitable for a weld bead morphology data set; 5, training the neural network, and predicting the weld bead morphology of the target process parameters; and 6, comparing the weld bead morphology predicted by the neural network with the verified weld bead morphology, and calculating a prediction error and precision. The method has the characteristics of high speed and high precision.

The invention relates to the technical field of unconventional oil and gas development, in particular to a system and a method for simulating gas-water alternation multi-turn pressing-braising injection and production oil displacement. According to the scheme, a pressing fracture network and a matrix reservoir are equivalently simulated, and pressing-braising injection and production of well soaking time are reasonably considered, so that the result is more accurate, and the oil displacement efficiency is improved. After the fracture core clamping unit and the matrix core clamping unit are exchanged in the forward direction and the reverse direction, a compact core is rapidly saturated, the oil reservoir initialization time is shortened, the influence of multiple fracture parameters on oil displacement in the three stages of pressing, braising and mining can be considered without repeatedly disassembling and assembling the core clamping unit, the fracture form is changed by replacing the fracture forming core, and the oil displacement efficiency is improved. The influence rule of a water-gas composite medium on the oil well pressing-braising-production capacity under different storage and seepage modes can be researched, the different storage and seepage modes comprise a multi-stage fracture-matrix reservoir, a large fracture-matrix reservoir, a micro-fracture-matrix reservoir and an original matrix reservoir, and independence and integrity of a fracture core and a matrix core are ensured; the experiment time is greatly shortened.

The invention relates to a method for detecting a B cell subset in a lymphatic tissue, a kit comprising a quantum dot-antibody CD19 conjugate, a quantum dot-antibody CD138 conjugate and a quantum dot-antibody CD1d conjugate is used for dyeing a lymphatic tissue section, and different antibodies are marked by quantum dots with different colors, so that the B cell subset in the lymphatic tissue section can be detected, and the B cell subset in the lymphatic tissue section can be detected. Content and positions of plasma cells and regulating B cells are confirmed, so that biological behaviors of the plasma cells and regulating B cells can be determined conveniently. According to the method, the existing multi-color immunofluorescence technology is improved, a secondary antibody dyeing link is omitted by using a quantum dot carrying antibody method, a direct method is adopted for marking, the advantages of quantum dots are exerted, the experiment time is saved, and the efficiency is improved.

The invention discloses a sample adjusting device for a microbiological detection analyzer, and relates to the technical field of microbiological detection analysis, the sample adjusting device comprises a device main body, an inner cavity of the device main body is fixedly connected with a fixing plate, the fixing plate is fixedly connected with a servo motor, an output shaft of the servo motor is fixedly connected with a rotating disc, and the rotating disc is fixedly connected with a rotating shaft of the rotating disc. A crank is rotationally connected to the rotating disc, a loading table is rotationally connected to the end, away from the rotating disc, of the crank, a guide rail is fixedly connected to an inner cavity of the device body, and a first sample tube and a second sample tube are arranged on the loading table; in the process that the test tube moves to the position below the first sample tube and the second sample tube, a piston rod of a piston barrel moves, detection liquid in a liquid storage barrel is dropped into the test tube, meanwhile, the dropping quantity of the detection liquid can be adjusted and controlled, errors caused by different persons in manual operation are avoided, and the detection accuracy is improved. In addition, direct contact between operators and reagents and samples is reduced in the automatic process, and the risks of cross contamination and environmental pollution are avoided.

The invention relates to a detection kit and a detection method for EBV latent infection of epithelial cells and B cells in a thymus and salivary gland tissue microenvironment, and the detection method comprises the following steps: dyeing thymus and salivary gland tissues by coupling quantum dots and a specific antibody; the conjugate of the quantum dots and the specific antibody is combined with a specific antigen of a tissue sample to realize marking of EBV latent infection epithelial cells and B cells by the quantum dots. According to the invention, the existing multicolor immunofluorescence technology is improved, the specific antibody and the quantum dot are coupled and purified and then are directly combined with the antigen on the tissue slice to realize direct marking of the quantum dot coupled antibody on the tissue sample, so that EBV latent infection cells in the thymus and salivary gland tissue microenvironment are positioned and quantified; the virus infection condition of the patient is evaluated from the pathogenesis level, the disadvantage that the previous evaluation means are limited is overcome, and the method has good sensitivity and accuracy.

The invention belongs to the technical field of environmental pollutant analysis and detection. The invention discloses a Fenton reaction-based efficient separation and extraction method for soil micro-plastics. The method comprises the following steps: preparing a micro-plastic soil sample; carrying out digestion and purification treatment on the micro-plastic; then separating the micro-plastics through a density flotation method; and finally, carrying out microscopic observation on the dried filter membrane by using a stereoscope so as to calculate the abundance of the micro-plastic and analyze the morphological characteristics of the micro-plastic. According to the method, the salt solution with lower cost and the organic matter digestion method with small influence on the micro-plastic particles are used, efficient removal of organic matter in the complex soil matrix and efficient extraction of the micro-plastic are achieved, the micro-plastic separation and extraction time is shortened, the micro-plastic recovery rate is increased, and the method is easy and convenient to operate, economical and practical.

The invention relates to a method for detecting Breg cells and Tfh cells in a chest adenoma tissue microenvironment, which comprises the following steps of: coupling quantum dots with a specific antibody, dyeing a chest adenoma tissue slice sample, and specifically connecting the specific antibody with antigens on the Breg cells and / or Tfh cells in the slice sample cells, so as to quantify or position the Breg cells and / or Tfh cells by the quantum dots. According to the method disclosed by the invention, the existing multicolor immunofluorescence technology is improved, and a secondary antibody dyeing link is omitted by using a method of carrying the antibody by the quantum dots, so that the efficiency is improved; the quantum dots are used for replacing fluorescent dye, direct marking of tissue slices can be achieved, local positioning of Breg cells and Tfh cells in a thymoma tumor microenvironment is achieved, and biological behaviors of the Breg cells and the Tfh cells can be determined conveniently; the immune condition can be evaluated by detecting the ratio condition of Breg / Tfh cells in the breast adenoma tumor microenvironment of a patient in vitro.

The invention relates to the field of semiconductor lasers and deep learning, and discloses a DFB laser spectral parameter reverse design method based on a DNN model, which comprises the following steps: giving spectral parameters of a DFB laser, and obtaining a threshold spectrum of the DFB laser through a transmission matrix method; extracting characteristic parameters of the threshold spectrum; recording values of the characteristic parameters and spectral parameter values of the corresponding DFB laser to generate a data set; initializing the weight and the back propagation error of the DNN model by adopting a particle swarm algorithm; inputting the data set into the initialized model for training; giving a target threshold spectrum, and extracting characteristic parameters; and inputting the characteristic parameters into the trained model, outputting spectral parameters of the DFB laser, and realizing reverse design of the spectral parameters. The method provided by the invention can reduce the tedious operation of acquiring the threshold spectrum by using a spectrograph for many times, improves the accuracy, and reduces the used time and memory proportion, thereby optimizing the spectral characteristics more quickly and more accurately.

The invention provides a medical packaging composite film and a preparation method thereof. The preparation method of the medical composite membrane comprises the following steps: S100, coating a first surface of an aluminum foil layer with an adhesive, attaching the first surface to a barrier material, and sequentially carrying out primary drying treatment and calendering treatment to obtain a barrier layer, an adhesive layer and the aluminum foil layer; s200, the second surface of the aluminum foil layer is coated with heat sealing slurry, secondary drying treatment is conducted, and the medical packaging composite film is obtained. The medical packaging composite film has excellent barrier property, biological safety, mechanical property and heat sealing property, and can provide reliable packaging protection for medical drugs.

The utility model relates to an ophthalmology instrument experiment animal platform which comprises an ophthalmology equipment adaptation frame and an animal platform body. The ophthalmology equipment adaptive frame comprises a column clamp, an adjusting frame and a base, the column clamp is used for clamping an ophthalmology equipment column, and the base is connected to the bottom of the adjusting frame and used for being supported on an ophthalmology equipment neck support; the animal platform is installed on the adjusting frame, the platform surface of the animal platform is located in the observation view field of the ophthalmology equipment in front of the adjusting frame, and an experimental animal fixing device is arranged on the animal platform; the experimental animal fixing device comprises an animal body fixing structure, a tooth fixing structure and a skull fixing structure to form a three-stage fixing system, so that the experimental animal is fixed and the eyes are still. The ophthalmological instrument experimental animal platform can be matched with common instruments for ophthalmological examination, experimental animals can be conveniently fixed, animal eyeballs are prevented from shaking and the like, the observation angle and distance of the eyeballs of the experimental animals can be accurately adjusted, the consistency of ophthalmological examination operation is guaranteed, and experimental animal imaging scientific research data are accurate.

The invention relates to a kit for detecting plasma cells and related antibody levels in a thymus microenvironment and application of the kit. The kit comprises a quantum dot labeled antibody CD138, a quantum dot labeled antibody CD21, a quantum dot labeled AchR, Musk and / or LRP4 receptor, an antigen repair solution and a DNA fluorescent dye solution, wherein the quantum dot labeled antibody CD138, the quantum dot labeled antibody CD21, the quantum dot labeled AchR, the quantum dot labeled Musk and / or the quantum dot labeled LRP4 receptor, the antigen repair solution and the DNA fluorescent dye solution; through the kit, one or more of plasma cells, follicular DC and related antibodies in a thymus (tumor) sample can be detected, so that the immune level of a tissue slice sample is obtained. A method of carrying the antibody by using the quantum dots is adopted, so that a secondary antibody dyeing link in the existing multicolor immunofluorescence technology is omitted, and the efficiency is improved; the quantum dots are used for replacing fluorescent dye, direct marking of tissue slices can be achieved, local positioning of plasma cells and follicular DCs in the thymoma tumor microenvironment is achieved, and both quantity detection and position detection can be achieved.

The invention provides an experimental method for measuring particle size distribution of particulate matter and a wet screening instrument.The wet screening instrument comprises a centrifugal screening barrel and a rotating mechanism, the centrifugal screening barrel comprises a barrel body, a screen assembly and a washing assembly are arranged in the barrel body, the screen assembly comprises a plurality of screen components which are arranged in a nested mode, and the washing assembly is connected with the rotating mechanism. A plurality of screen holes are formed in the side wall of the screen component; the screen mesh apertures of the screen mesh components are sequentially reduced from the middle part of the screen mesh assembly to the outside; an annular channel used for receiving accumulated materials on the screen is formed between every two adjacent screen components. The flushing assembly is located on the outer side of each screen component, and a water spraying opening of the flushing assembly faces the outer side wall of the screen component; the rotating mechanism is connected with the screen assembly and drives the screen assembly to rotate. The rotating mechanism is fixedly connected with the screen assembly, and a water outlet is formed in the bottom of the barrel body. By adopting the technical scheme of the invention, accurate and efficient measurement of particle size distribution of particles is realized, and the experiment precision and the experiment efficiency are improved.

According to the device and method for measuring the apparent heat conductivity coefficient of the powder at the low temperature, the G-M refrigerating machine is adopted for providing the cold boundary temperature, and compared with a traditional method for providing the cold boundary temperature through low-temperature liquid, the device and method for measuring the apparent heat conductivity coefficient of the powder at the low temperature have the advantage that measurement accuracy is improved. According to the invention, a large amount of low-temperature liquid can be saved, the experiment time can be greatly shortened, a lower cold boundary temperature can be reached, and the cold boundary temperature can be as low as 4.2 K, so that the measurement of a lower temperature can be realized. Moreover, a heater is arranged for the second-stage cold head, the second-stage cold head is heated when needed, the cold boundary temperature can be adjusted, and therefore powder apparent heat conductivity coefficient measurement under the wide temperature zone (4.2-400 K) can be achieved. The device can realize the measurement of a liquid hydrogen temperature zone and a wide temperature zone, the measurement is accurate and rapid, and the cost is saved.

The invention discloses a device and method for measuring the photothermal effect of a two-dimensional material, and the device comprises an optical bread board, the optical bread board is provided with a light heating module, an electric heating module and a measurement module, the measurement module is provided with a to-be-measured material, and is used for supporting the to-be-measured material and obtaining the temperature of the to-be-measured material; the electric heating module is used for heating a to-be-tested material and obtaining the temperature in the heating process; the light heating module is used for illuminating a to-be-tested material and acquiring light power during illumination; the measurement principle advantage of the measurement device is remarkable, the Newton's cooling law is used as the core calculation principle, a physical model for calculating the photothermal conversion efficiency is simplified, the measurement device can calculate the photothermal effect of the two-dimensional material more visually and accurately, the measurement cost can be effectively reduced, and the measurement operation can be simplified.

The invention provides a micro-droplet collecting device and a method for collecting micro-droplets by using the device. The micro-droplet collecting device comprises a collecting part which is provided with a liquid distributing pipe, a plurality of collecting pipes and a plurality of liquid conveying pipes communicated with the liquid distributing pipe and the collecting pipes; a receiving opening for receiving micro-droplets is formed in the top of the liquid distribution pipe, and a plurality of liquid distribution openings corresponding to the collecting pipes are uniformly formed in the position close to the bottom in the circumferential direction; the top of the collecting pipe is provided with a collecting opening and a sealing cover matched with the collecting opening; one end of the liquid conveying pipe is limited on the inner side of the collecting opening and penetrates into the bottom of the collecting pipe through the collecting opening, and the other end of the liquid conveying pipe is communicated with the liquid separating opening corresponding to the liquid separating pipe; the fixing part is detachably mounted on the working platform; and the liquid distribution pipe is detachably placed and limited on the fixing part. When the method for collecting the micro-droplets is used for collecting the micro-droplets, tube replacement and oil replacement are not needed, the quality of the droplets is uniform and stable, and the experiment time is saved.

The application discloses a kind of high-pressure fuel filter prediction model construction method and flow prediction method, including two prediction models, method specifically includes: using computational fluid dynamics method carries out fluid dynamics simulation calculation to high-pressure fuel filter, constructs working condition sample data set;The size of filter hole, quantity, filter working pressure, fuel viscosity and filter pressure drop are used as input variable, and filter flow is used as output variable, two new high-pressure filter general nonlinear flow prediction models are established using artificial neural network and regression prediction method respectively, the flow characteristics of different models of filter in working interval can be accurately predicted using the neural network prediction method proposed in the application at any working condition point, and the error is low;The flow of different models of filter under different conditions can be quickly and accurately obtained, the accuracy meets the engineering needs, while greatly shortening the experimental time and calculation time, saving design cost.

The invention discloses a microwave efficient paraffin section manufacturing method for animal tissues and application of the microwave efficient paraffin section manufacturing method. The microwave efficient paraffin section manufacturing method comprises the following steps of sampling, fixing, dewatering, transparentizing, paraffin soaking, embedding, slicing, section unfolding, dyeing, section sealing and observing. According to the method disclosed by the invention, by combining a microwave technology and aiming at the characteristics of zebra fish intestinal tissue materials, the steps of fixing, dehydrating, transparentizing, waxing and the like in the operation process are optimized and improved, so that the experiment time is greatly shortened; taking zebra fish intestinal tissues as an example, the traditional paraffin section preparation needs about 100 hours, but the paraffin section preparation method disclosed by the invention only needs 6 hours, so that the rapid paraffin section preparation is realized, the experiment time is greatly shortened, and the intestinal tissue section with a complete structure can also be obtained.

The present invention discloses an intelligent water reducer tester, which relates to the field of water reducer experiments. An intelligent water reducer tester includes a base, and also includes: a rotating frame, which is rotatably connected to the base through a first rotating shaft; a rotating seat, which is equidistantly and rotatably connected to the rotating frame; an experimental cylinder, which is installed on the rotating seat; and a spiral propulsion blade, which is fixedly connected to the inner wall of the experimental cylinder. The present invention can conduct a relatively accurate experiment on the optimal addition content of the water reducer through the coordinated use of the base, the rotating frame, the rotating seat, the experimental cylinder, the spiral propulsion blade, the first hydraulic telescopic cylinder, the drawer box, the main machine and other components, so as to facilitate the acquisition of the optimal water reducer addition content, and the degree of automation is also relatively high, which effectively saves the experimental time.

The invention relates to the technical field of biology, in particular to a DNA tag library construction method based on droplet sorting and a magnetic microsphere technology, according to the method, the concentration of DNA tag template sequences is adjusted through Poisson distribution, it is ensured that each droplet contains at most one DNA tag template sequence, the problem that multiple templates are mixed into the droplets can be solved, and the DNA tag library construction efficiency is improved. The method has the advantages that the method is simple and convenient to operate, label repetition and collision are avoided, uniqueness of DNA labels in liquid drops is guaranteed, DNA label sequences after PCR amplification are further captured through magnetic microspheres, it is guaranteed that the amplified DNA labels can be efficiently recycled and purified, interference of non-specific reaction in PCR amplification is avoided, and the method is suitable for large-scale popularization and application. The combination of the technical points ensures the application advantages of the technical scheme in high-throughput sequencing, including diversity, accuracy and high efficiency of tags.

The application belongs to the technical field of medicine, and particularly relates to a method for embedding micro-organoids or cells with agarose. The method for embedding micro-organoids or cells with agarose comprises the following steps: 1) fixing micro-organoids or cells with a fixing solution; 2) washing the fixed organoids or cells, removing supernatant after centrifugation, and obtaining organoid or cell precipitate; 3) pre-embedding organoids or cells with agarose; 4) taking out the agarose pre-embedded organoids or cells after demolding, and transferring the agarose pre-embedded organoids or cells to an embedding box, and sequentially performing ethanol gradient dehydration, transparent treatment and paraffin wax immersion treatment on the sample according to a specific procedure; and 5) paraffin embedding. Compared with the prior art, the application has the following beneficial effects: improving organoid / cell density; by agarose embedding, reducing the relative dispersion volume of organoids / cells, significantly improving the density of the sample in the slice, facilitating observation and detection, and having good detection effect.

The invention discloses an embryo teratogenic substance screening prediction model based on human induced pluripotent stem cells and a construction method thereof, the embryo teratogenic substance screening prediction model is cells and / or cell aggregates, the cells are myocardial cells differentiated from the human induced pluripotent stem cells, and the cell aggregates are embryoid bodies. The embryo teratogenic substance screening prediction model uses human induced pluripotent stem cells to more accurately simulate the environment of human early embryonic development, avoids species difference of animal models, provides a relatively rapid and accurate screening platform, is suitable for high-throughput drug screening, and has good application prospects. Compared with the prior art, the kit has higher sensitivity and specificity, is helpful for eliminating compounds with potential reproductive toxicity in the early stage of drug development, and reduces the risk of later clinical test failure.

The invention discloses a multiple immunofluorescence imaging application of a nucleic acid aptamer in malignant tumor tissue, and relates to the field of tissue immunofluorescence technology. The nucleic acid aptamer includes Sgc8c, SL1 or SYL3C. The invention discloses a nucleic acid aptamer for pathological detection of malignant tumors, which provides an effective detection method for pathological diagnosis of tumors; the method is simple, rapid, sensitive and accurate; the immunofluorescence technology has strong specificity, high sensitivity, simple operation and good positioning performance in tissues.

The invention discloses a soil particle mesoscopic parameter calibration method which is characterized by comprising the following steps: step 1, applying a predetermined load to a soil sample, and obtaining an actual soil settlement amplitude under the predetermined load; step 2, acquiring a soil sample image and extracting geometric parameters of soil particles; 3, soil particle mesoscopic parameters are input; 4, according to the actual soil settlement amplitude in the step 1, the soil particle geometric parameters in the step 2 and the soil particle mesoscopic parameters input in the step 3, calculating the soil particle mesoscopic parameters through an iterative optimization algorithm; and step 5, outputting the calculated soil particle mesoscopic parameters as calibrated soil mesoscopic parameters, and through the high-resolution image and the iterative optimization algorithm, the accuracy of soil particle mesoscopic parameter calibration is improved, and the calibration precision is improved.

The application discloses a dielectric breakdown field strength and temperature dependence simulation method and system, and the simulation method comprises the following steps: establishing a criterion for polymer damage caused by molecular chain slip under the action of heat based on a free volume theory; establishing a breakdown criterion for polymer damage caused by molecular chain slip under the joint action of an electric field and a thermal field; quantifying the serpentine movement of a molecular chain under the action of a strong force; establishing an approximate analytical expression of polymer breakdown strength; and fitting the experimental results of sample breakdown field strength change with temperature according to the analytical expression by using a Curve fitting tool of Matlab. The breakdown model established from the perspective of molecular chain displacement can well explain the change relationship between the polymer nanocomposite dielectric breakdown field strength and temperature, and provides theoretical guidance for the preparation of high-temperature dielectric materials.

The utility model discloses a coarse-grained soil dry-wet cycle shear testing machine which comprises a test bed, a sliding rail is arranged at the upper end of the test bed, a detachable lower shear box is connected to the sliding rail in a sliding mode, an upper shear ring is arranged at the upper end of the lower shear box, and a horizontal servo electric cylinder is installed at the end, located at the sliding rail, of the test bed. The horizontal servo electric cylinder is provided with a horizontal load sensor, the horizontal load sensor is provided with a push-pull shaft, and the test bench is provided with a displacement sensor at the other end of the slide rail; the upper end of the test bed is connected with a mounting frame, a vertical servo electric cylinder is downwards mounted on the mounting frame, a vertical load sensor is downwards arranged on the vertical servo electric cylinder, a rotating motor is arranged at the lower end of the vertical load sensor, and the test bed further comprises a pressing disc and a rotating disc which are detachably connected with the rotating motor. A plurality of hollow stirring rods with holes are arranged at the lower end of the rotating disc. When the coarse-grained soil is subjected to dry-wet cyclic shearing, a sample does not need to be taken out, and the sample can be saturated by directly stirring and injecting water, so that the experiment efficiency is effectively improved.

The dewaxing repair liquid is prepared from the following components in parts by mass: 10 to 20 parts of a water-soluble dewaxing agent, 0.1 to 0.5 part of an antigen repair agent, 10 to 20 parts of a tissue clearing agent, 0.1 to 0.5 part of a tissue penetrating agent, 0.5 to 1 part of a PH (Potential of Hydrogen) stabilizer and the balance of water. The dewaxing repair liquid disclosed by the invention can be used for directly carrying out antigen repair without dewaxing and hydration in advance, so that the operation steps are shortened, the experiment time is saved, and the effect is not obviously different from that of step-by-step dewaxing, hydration and repair.

The invention discloses a method for simultaneously determining (+)-2-aminobutanol and ethambutol hydrochloride based on an RP-HPLC-NQAD method, and particularly relates to the technical field of drug detection. The method comprises the following steps: preparing standard curves of ethambutol hydrochloride and (+)-2-aminobutanol; taking the ethambutol hydrochloride tablets, and grinding the ethambutol hydrochloride tablets into powder to obtain ethambutol hydrochloride tablet powder; adding purified water into the ethambutol hydrochloride tablet powder, carrying out ultrasonic dissolution, and respectively diluting the ethambutol hydrochloride tablet powder to 2 mg / ml and 0.25 mg / ml with water to respectively obtain two loading analysis solutions; and detecting the upper analysis liquid by a reversed-phase high performance liquid chromatography tandem hydrogel particle laser technology detector, and calculating to obtain the concentrations of ethambutol hydrochloride and (+)-2-aminobutanol. The method has the advantages that the content of ethambutol hydrochloride and the content of (+)-2-aminobutanol compounds can be detected at the same time, results do not interfere with each other in the detection process of the two compounds, derivation is not needed in pretreatment, accuracy is high, sensitivity is high, and durability is good.