54results about How to "Shorten experiment time" patented technology

The invention discloses a capture probe group for detecting pathogenic microorganisms and application of the capture probe group. The nucleic acid sequence of the capture probe group comprises a sequence as shown in SEQ ID NO. 1 to SEQ ID NO. 101. The capture probe group can cover and detect 119 types of bacteria, 32 types of fungi, 41 types of DNA viruses and 71 types of RNA viruses and covers common infectious disease pathogens, the kit containing the capture probe can cover and detect 88 types of drug-resistant and virulence genes, the detection performance of pathogenic microorganisms is greatly improved, and the sensitivity and specificity are improved.

The invention provides a residual stress prediction method considering ultrasonic peening process parameters of a welded joint, and the method specifically comprises the following steps: carrying out thermal-mechanical coupling simulation of a welded structure based on a finite element method, obtaining a residual stress field of the welded structure, taking the residual stress field as initial stress, and building an ultrasonic peening processing finite element model; on the basis of a hypercube Latin sampling method, a parameter combination sample is extracted, corresponding residual stress data after ultrasonic peening are obtained through simulation, and a residual stress prediction function model containing an undetermined coefficient is established with ultrasonic peening parameters as independent variables. And based on a particle swarm optimization algorithm, training to obtain an undetermined coefficient value which enables a prediction effect to be optimal, and completing establishment of a prediction function model. Therefore, the optimal process parameter combination of ultrasonic peening can be obtained so as to improve the mechanical property of a welded joint. According to the residual stress prediction method considering the ultrasonic impact process parameters of the welding joint, a basis is provided for improving the service performance of a large-scale thin-wall part welding structure.

The application discloses a kind of high-pressure fuel filter prediction model construction method and flow prediction method, including two prediction models, method specifically includes: using computational fluid dynamics method carries out fluid dynamics simulation calculation to high-pressure fuel filter, constructs working condition sample data set;The size of filter hole, quantity, filter working pressure, fuel viscosity and filter pressure drop are used as input variable, and filter flow is used as output variable, two new high-pressure filter general nonlinear flow prediction models are established using artificial neural network and regression prediction method respectively, the flow characteristics of different models of filter in working interval can be accurately predicted using the neural network prediction method proposed in the application at any working condition point, and the error is low;The flow of different models of filter under different conditions can be quickly and accurately obtained, the accuracy meets the engineering needs, while greatly shortening the experimental time and calculation time, saving design cost.

The application belongs to the technical field of medicine, and particularly relates to a method for embedding micro-organoids or cells with agarose. The method for embedding micro-organoids or cells with agarose comprises the following steps: 1) fixing micro-organoids or cells with a fixing solution; 2) washing the fixed organoids or cells, removing supernatant after centrifugation, and obtaining organoid or cell precipitate; 3) pre-embedding organoids or cells with agarose; 4) taking out the agarose pre-embedded organoids or cells after demolding, and transferring the agarose pre-embedded organoids or cells to an embedding box, and sequentially performing ethanol gradient dehydration, transparent treatment and paraffin wax immersion treatment on the sample according to a specific procedure; and 5) paraffin embedding. Compared with the prior art, the application has the following beneficial effects: improving organoid / cell density; by agarose embedding, reducing the relative dispersion volume of organoids / cells, significantly improving the density of the sample in the slice, facilitating observation and detection, and having good detection effect.

The invention discloses a soil particle mesoscopic parameter calibration method which is characterized by comprising the following steps: step 1, applying a predetermined load to a soil sample, and obtaining an actual soil settlement amplitude under the predetermined load; step 2, acquiring a soil sample image and extracting geometric parameters of soil particles; 3, soil particle mesoscopic parameters are input; 4, according to the actual soil settlement amplitude in the step 1, the soil particle geometric parameters in the step 2 and the soil particle mesoscopic parameters input in the step 3, calculating the soil particle mesoscopic parameters through an iterative optimization algorithm; and step 5, outputting the calculated soil particle mesoscopic parameters as calibrated soil mesoscopic parameters, and through the high-resolution image and the iterative optimization algorithm, the accuracy of soil particle mesoscopic parameter calibration is improved, and the calibration precision is improved.

The application discloses a dielectric breakdown field strength and temperature dependence simulation method and system, and the simulation method comprises the following steps: establishing a criterion for polymer damage caused by molecular chain slip under the action of heat based on a free volume theory; establishing a breakdown criterion for polymer damage caused by molecular chain slip under the joint action of an electric field and a thermal field; quantifying the serpentine movement of a molecular chain under the action of a strong force; establishing an approximate analytical expression of polymer breakdown strength; and fitting the experimental results of sample breakdown field strength change with temperature according to the analytical expression by using a Curve fitting tool of Matlab. The breakdown model established from the perspective of molecular chain displacement can well explain the change relationship between the polymer nanocomposite dielectric breakdown field strength and temperature, and provides theoretical guidance for the preparation of high-temperature dielectric materials.

The utility model discloses a coarse-grained soil dry-wet cycle shear testing machine which comprises a test bed, a sliding rail is arranged at the upper end of the test bed, a detachable lower shear box is connected to the sliding rail in a sliding mode, an upper shear ring is arranged at the upper end of the lower shear box, and a horizontal servo electric cylinder is installed at the end, located at the sliding rail, of the test bed. The horizontal servo electric cylinder is provided with a horizontal load sensor, the horizontal load sensor is provided with a push-pull shaft, and the test bench is provided with a displacement sensor at the other end of the slide rail; the upper end of the test bed is connected with a mounting frame, a vertical servo electric cylinder is downwards mounted on the mounting frame, a vertical load sensor is downwards arranged on the vertical servo electric cylinder, a rotating motor is arranged at the lower end of the vertical load sensor, and the test bed further comprises a pressing disc and a rotating disc which are detachably connected with the rotating motor. A plurality of hollow stirring rods with holes are arranged at the lower end of the rotating disc. When the coarse-grained soil is subjected to dry-wet cyclic shearing, a sample does not need to be taken out, and the sample can be saturated by directly stirring and injecting water, so that the experiment efficiency is effectively improved.

The utility model relates to the field of pesticide residue treatment, specifically is a kind of integrated agricultural product pesticide residue crushing extraction equipment, including extraction cylinder, the inside of extraction cylinder is sequentially opened with crushing chamber, control chamber and stirring chamber from top to bottom, heating unit is fixedly arranged on extraction cylinder and is matched with stirring chamber, the side portion of extraction cylinder is provided with centrifuge, and the bottom end of stirring chamber is communicated with centrifuge;The integrated agricultural product pesticide residue crushing extraction equipment of the utility model integrates multiple functions such as crushing, stirring, heating, centrifugal separation in one, realizes the full-process automation operation from agricultural product feed to residue extraction, separation, reduces the connection and transmission steps between multiple devices in traditional equipment, improves work efficiency, saves space.

The invention discloses a metal fatigue testing machine and a fatigue testing method, which can test the bending fatigue strength of a rectangular thin-plate metal specimen. By clamping the two ends of the specimen and applying a load, under the action of an alternating load, the stress level of the specimen is lower than the yield limit of the material, and after a long period of repeated stress cycles, the bending fatigue strength of the rectangular thin-plate metal specimen is tested. The load and bending stress can be adjusted, and the frequency can be adjusted at will. It saves electricity and does not require a water source, and has a low cost of use, and the power of a general motor is only a few kilowatts or a few hundred watts. It can realize high-load, high-efficiency, high-precision, and low-consumption experiments, thereby shortening the experimental time and reducing the experimental cost. The parts are less damaged and have a long service life, and the motor that is almost maintenance-free can be used uninterruptedly for a long time. The installation and maintenance are simple and convenient, and there is no need to frequently stop the machine to replace hydraulic oil and the like, and the subsequent motor maintenance cost is almost zero, and the maintenance cost is low. It uses electric energy, which is clean and pollution-free and stable.

A quantitative experiment research analysis method for a nerve stem action potential non-stress period phenomenon comprises the following steps: step 1, selecting a plurality of different double-pulse wave interval time values t as experiment conditions, and carrying out an experiment sub-step 1, an experiment sub-step 2 and an experiment sub-step 3 aiming at each wave interval time value t; step 2, superposing the neural stem AP waveform data obtained in the experimental sub-step 1 and the experimental sub-step 3, dividing the superposed neural stem AP waveform data by 2, and representing the neural stem AP waveform data AP1 induced by the first pulse electrical stimulation in the double-pulse electrical stimulation in the experimental sub-step 2 by using the result; and step 3, subtracting the neural stem AP waveform data AP1 induced by the first pulse electrical stimulation in the experiment sub-step 2 from the mixed waveform data APall obtained in the experiment sub-step 2. According to the method, the two neural stem AP waveforms AP1 and AP2 induced by the first pulse and the second pulse respectively can be obtained, and the critical value ranges of the absolute refractory period, the relative refractory period and the refractory phenomenon-free period can be accurately determined.

The present invention provides a fully automatic abdominal withdrawal reflex scoring method and device, including: collecting real-time image data of experimental animals and preprocessing to obtain raw data images, automatically judging whether the current state of the experimental animals is a frequently active state or a resting state according to the raw data images; if it is a resting state, controlling a micropump to slowly inject a set dose of liquid into a balloon, and collecting real-time image data of the experimental animals in a resting state at the same time; preprocessing the collected image data of the experimental animals in a resting state to obtain a number of raw data images; and automatically scoring the abdominal withdrawal reflex of the experimental animals according to the raw data images. The present invention avoids the problems of traditional manual operation and strong subjectivity of manual scoring by automatically timing, automatically injecting, and automatically scoring multiple experimental animals, thereby improving the efficiency of the experiment and the accuracy of the scoring.

The present invention relates to a thymus and salivary gland tissue microenvironment epithelial cells and B cells EBV latent infection detection kit and detection method, by coupling quantum dots and specific antibodies, staining in thymus and salivary gland tissue; quantum dots and specific antibody conjugates are combined with tissue sample specific antigens to achieve quantum dots to EBV latently infected epithelial cells and B cells marking. The present invention improves the existing multicolor immunofluorescence technology, and uses specific antibodies and quantum dots after coupling and purification, and directly combines with antigens on tissue sections to achieve quantum dot-coupled antibodies to directly mark tissue samples, thereby locating and quantifying EBV latently infected cells in the thymus and salivary gland tissue microenvironment, and evaluating the patient's viral infection from the pathogenesis level, overcoming the disadvantages of the limited evaluation methods in the past, and having good sensitivity and accuracy.

This invention relates to a variable-order, variable-capacity water tank. The technical problem this invention aims to solve is to provide a nonlinear, multi-purpose water tank with variable order and variable capacity. The technical solution adopted by this invention includes a water tank body, a sealing plate, a first insert plate, a second insert plate, and a third insert plate. The water tank body includes a bottom plate and a tank casing. The tank casing is divided into a first inlet chamber, a first outlet chamber, an overflow chamber, a second outlet chamber, a second inlet chamber, a first working chamber, a second working chamber, and a third working chamber by a water tank partition. Water passage holes are provided between the first inlet chamber and the first working chamber, and between the second inlet chamber and the third working chamber. The advantages of this invention are: the water tank structure is ingeniously designed, allowing for variable capacity and variable order, enabling the construction of mathematical models of various orders; the structure of the sealing plate and insert plates replaces the traditional manual valve structure, exhibiting more significant nonlinear characteristics, enabling the system to stabilize quickly, shortening experimental time, and increasing the controllable range.

The invention discloses a testis organ as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing printing ink and carrying out photocuring 3D printing, wherein the printing ink comprises photocuring hydrogel and testicular cells. The testicular organ has similar cell type composition, morphology and expression of a cell specific marker as the testicular organ. The method for preparing the testicular organ is easy to operate, rapid and convenient, and the 3D cultured testicular organ is formed through the 3D photocuring printing technology. The testicular organ can be used for evaluating the reproductive toxicity of a compound such as a medicine to male, and has reliability and wide application prospect.

The utility model belongs to the technical field of biological sample treatment, and provides a reagent extraction assembly which comprises a kit and a magnetic rod group, the magnetic rod group is mounted above the kit and can move in the kit; the kit comprises a plurality of hole groups, the hole groups are arranged side by side, the column number of the hole groups is not less than one, and the row number is not less than one; each hole group is provided with a sample hole, four washing liquid holes and two elution holes, the four washing liquid holes are arranged in a shape like a Chinese character'tian ', the two elution holes are arranged on one side of the four washing liquid holes side by side in a shape like a Chinese character'ri', the sample hole is arranged on the other side of the four washing liquid holes, and the width of the sample hole corresponds to the total width of two adjacent washing liquid holes. According to the utility model, a plurality of hole groups are arranged side by side, so that a plurality of samples are allowed to be processed at the same time, and the experiment processing capacity and efficiency are remarkably improved. Due to the specific design of the interior of each hole group, the sample holes have enough stirring space, meanwhile, the washing and elution processes are more efficient, and the experiment time is shortened.

The utility model discloses a kind of BNCT mouse irradiation devices without anesthesia, including disc body, sliding assembly is installed on disc body, detachably installed with mouse fixer on sliding assembly, mouse fixer includes head fixing assembly and tail fixing assembly for limiting mouse activity, fixed shaft is vertically arranged on disc body, bracket is installed on fixed shaft, the end portion of bracket is located above mouse fixer and is provided with reversible folding connecting plate. Mouse head and neck are fixed by the fastening screw of mouse fixer, mouse tail is fixed by clamping groove, mouse can be irradiated subsequently without anesthesia, avoid anesthetics to influence BNCT treatment effect;By round multi-arm sliding rail clamping disc, multiple mouse fixers can be assembled, one mouse is placed in each fixer, reasonably divide area, avoid multiple mice confusion;By adjusting the position of mouse fixer on sliding rail and turning L-shaped connecting plate, multiple mice can be irradiated at different parts simultaneously, convenient operation, save experimental time.

The utility model provides a rotary evaporator capable of reducing heat loss, which relates to the technical field of rotary evaporators and comprises a bottom plate and a water bath kettle, a mounting rack is fixedly mounted at the top of the bottom plate, and a rotating mechanism is obliquely arranged on one side of the mounting rack. According to the electric cooker, the support, the sliding groove, the second motor, the lead screw, the connecting block, the transparent heat preservation pot cover and other structures are arranged, the second motor is started, the second motor can drive the lead screw to rotate when working, and the lead screw can drive the connecting block to slide downwards in the sliding groove in the rotating process; the connecting block can drive the transparent heat preservation pot cover to move downwards together in the downward sliding process, the transparent heat preservation pot cover and the water bath pot can be combined together after moving by a certain distance, heat preservation can be rapidly conducted on the water bath pot through the structure, then the phenomenon that heat is prone to being lost in the heating process is effectively avoided, the evaporation speed of a solvent is increased, and the service life of the water bath pot is prolonged. And the experiment time is shortened.

The present invention relates to a DNA extraction centrifugal disk. The DNA extraction centrifugal disk comprises at least one DNA extraction unit; the front of the DNA extraction unit is provided with a first liquid inlet, a second liquid inlet, a third liquid inlet, a temporary liquid storage area and a filtration area, and the back is provided with a first liquid storage area and a second liquid storage area; a DNA adsorption material is provided in the filtration area; the first liquid inlet and the second liquid inlet are respectively connected to the filtration area through a flow channel; the third liquid inlet is connected to the temporary liquid storage area through a flow channel, and the temporary liquid storage area is connected to the filtration area through a flow channel; the second liquid storage area is connected to the first liquid storage area through a flow channel, and the first liquid storage area is connected to the filtration area through a flow channel. The DNA adsorption material is preferably a silica gel membrane. The present invention integrates the reaction process of the silica gel membrane adsorption method into the centrifugal disk, which can efficiently realize DNA extraction.

The invention provides a micro-fluidic chip for cell identification, cell culture and drug response monitoring and a use method. The chip comprises a substrate, a first electrode, a second electrode, a third electrode and a fourth electrode, the cell injection layer is arranged on the substrate, and a cell recognition assembly is arranged on the cell injection layer and used for recognizing the type of injected cells; a cell monitoring component is arranged on the cell injection layer and is used for culturing injected cells, performing different medication on the cultured injected cells according to the types of the injected cells, and monitoring the medication reaction of the cultured injected cells. According to the micro-fluidic chip for identifying and culturing the cells and monitoring the medication reaction and the use method of the micro-fluidic chip, the cell type is identified through the cell identification assembly, medication is determined according to the cell type, targeted medication is performed on different types of cells through the cell monitoring assembly, and the medication reaction is monitored, so that the medication reaction of the cells is monitored, and the accuracy of the medication reaction is improved. The micro-fluidic chip integrating identification and medication monitoring is realized.

The invention provides a device and a method for simulating biochemical reaction of hydrogen in a multi-physics coupling environment, and relates to the technical field of hydrogen reaction devices. Solid reactants and liquid reactants can be placed respectively, reaction experiments of various combinations of hydrogen and different reactants can be achieved according to corresponding requirements, conditions such as internal temperature and humidity are adjusted in cooperation with the temperature and humidity adjusting module, a real reaction scene is simulated, and the experiment efficiency is improved. A platform is provided for researching the interaction between hydrogen and corresponding reactants such as rocks, microorganisms and solutions, different research requirements are met, and research conditions are provided for researching the stability and leakproofness of underground hydrogen storage.

The utility model belongs to the field of atom inertia measurement, and particularly discloses an auxiliary device for evaluating the Coriolis force of an atom interferometer, the auxiliary device comprises a lifting rotation supporting module and an inclination detection adjusting module, and the inclination detection adjusting module comprises an inclination detection unit and an inclination adjusting unit; the inclination detection unit is used for detecting the inclination of the vacuum container; the inclination adjusting unit is located at the bottom of the vacuum container and used for supporting the vacuum container and adjusting the vacuum container to be horizontal according to the inclination. The lifting rotation supporting module can be coaxially arranged under a vacuum container of the atom interferometer and can drive the vacuum container to rotate so as to modulate the Coriolis force. According to the invention, the effect of rotating the vacuum container in the atom interferometer with high efficiency and high precision can be realized.

The present application relates to dilution refrigerator temperature calibration technical field, and disclose a kind of dilution refrigerator thermometer in situ calibration system based on natural cooling dynamics, comprising: according to the dynamics equation of cooling power and temperature Curvature is calculated, according to the temperature corresponding to thermometer resistance value, the temperature is substituted into temperature curvature, and the calibration function of resistance value and temperature is obtained;Identify the multivalued region of temperature, and evolve multiple branch functions in multivalued region in calibration function, calculate the deviation measure of each branch function and calibration function;In multivalued region, the support degree of each branch function to calibration function is calculated, and real-time data is obtained by adjusting calculation load, to minimize the number of experiments to verify calibration function;According to the branch verification result of multivalued region, dynamically adjust the verification strategy of non-multivalued region, under the premise of not relaxing calibration accuracy, through dynamics branch and load guide, significantly reduce the repeated experiment cost of dilution refrigerator cryogenic calibration.

The invention discloses a working system S3-S5 motor temperature rise test method and device capable of shortening the interruption period, and relates to the technical field of motor test. A long-term problem is split into a plurality of independent short-term problems by the PPINN model, and the short-term problems are supervised by a rapid coarse-grained (CG) solver, so that the high calculation efficiency of training the neural network by using small data sets can be utilized to the maximum extent, the training time can be shortened, a big data set can be decomposed into a plurality of small data sets for training by the PPINN model, and the training efficiency is improved. Therefore, under the condition of not enough GPU resources, the PINN can still be trained by using a big data set on the CPU resources, data acquired by the equivalent temperature rise test is used for prediction model training and prediction of equivalent motor temperature rise test time and a temperature rise value, only one time of measurement is needed for one time of temperature rise test, and compared with a traditional method, the experiment time can be greatly shortened, and the test efficiency is improved. The test efficiency is improved, and the labor intensity and cost are reduced.

This utility model relates to the field of anti-icing coating testing technology, and provides an anti-icing coating testing device using rapid cooling with liquid carbon dioxide. The device includes an aluminum substrate, an aluminum vaporization grid, and a test sample. The test sample is positioned on the top of the aluminum substrate, and the aluminum vaporization grid is positioned on the bottom of the aluminum substrate. A liquid carbon dioxide inlet is located in the middle of the left side of the aluminum vaporization grid, and a carbon dioxide outlet is located in the middle of the right side of the aluminum vaporization grid. This utility model utilizes the principle of heat absorption during liquid carbon dioxide vaporization to rapidly reduce the temperature of the aluminum substrate in a short time, quickly simulating the icing / icing phenomenon under actual extreme low-temperature environments. Compared to traditional testing methods, this significantly shortens the testing cycle, allowing for rapid testing of the anti-icing coating performance, effectively improving testing efficiency, and saving considerable time and costs for research and development and production. Simultaneously, the realistic simulation of extreme environments enhances the practicality and reference value of the test results.

The invention belongs to the field of high-molecular polymers, and relates to a multichannel confocal rapid phase diagram instrument, application and a polymer solution system phase diagram drawing method. The multichannel confocal rapid phase diagram instrument comprises the following components which are arranged in sequence: a laser light source, a laser beam expander, a first lens, a multichannel precise temperature control sample cell system and a detection system, wherein the detection system comprises a small-angle confocal diode detection system; the small-angle confocal diode detection system comprises a second lens with a first collimation system and a photodiode with a second collimation system, and the first lens and the second lens form a double-lens 2f-2f confocal optical system. According to the device and the method, the phase transition rule of a plurality of polymer solution systems in the heating process can be monitored at the same time, and theoretical guidance and regulation and control basis are provided for regulating and controlling the structure or performance of macromolecules by regulating the properties, the phase transition, the temperature and the like of a solvent in the solution polymerization process and the solution processing process.

The present invention discloses a precision low-temperature thermostat temperature control method based on linear auto-disturbance rejection, establishes a heat transfer physical model for a general low-temperature thermostat, and provides an auto-disturbance rejection control method suitable for the low-temperature thermostat in combination with the physical model, which can effectively improve the anti-disturbance capability of the parameters, reduce the overshoot of the temperature control, and achieve rapid control of the target temperature. The present invention designs an extended state observer based on the linear auto-disturbance rejection control method, improves the dynamic performance and steady-state performance of the low-temperature thermostat, solves the problems of large overshoot, small applicable temperature range of parameters, and poor anti-disturbance capability of conventional PI or PID control methods, enables the low-temperature thermostat to achieve a wider range of temperature regulation, improves temperature control efficiency, and saves experimental time.

The present invention discloses a method for preparing samples for PPMS specific heat measurement, which can improve the efficiency of sample preparation for PPMS specific heat measurement and shorten the experiment time. The method of the present invention rolls copper used for increasing the thermal conductivity of the sample and improving the heat transfer efficiency into concentric copper strips of non-equal diameter and puts them into a copper cup for holding the sample. Compared with the existing method for preparing samples for PPMS specific heat measurement, the method of the present invention is more convenient and efficient in the sample preparation process, can simplify the preparation process, and the specific heat test result of PPMS is consistent with the prior art, and does not affect the accuracy of the experiment.

The invention discloses a cell culture medium configuration optimization method, device and equipment and a storage medium, and the method comprises the following steps: training a target agent model based on a current component data set to obtain a current prediction model, and determining a current component collection function; acquiring a target component configuration space based on the current component acquisition function to obtain multiple pieces of second component configuration information; based on the current component data set, the second component configuration information and a second experiment result corresponding to each piece of second component configuration information, determining a to-be-selected component data set obtained by the current optimization; adding the to-be-selected component data set obtained by the current optimization into the current component data set, and returning to execute the operation of training the target agent model based on the training data; and the optimal target component data set meeting the preset convergence condition is obtained. Through the technical scheme of the embodiment of the invention, comprehensive and efficient optimization of the cell culture medium is realized, so that the optimization degree and efficiency of the cell culture medium are improved.

The invention relates to the technical field of biology, in particular to a reagent and a method for screening a 3-sialyltransferase mutant through a high-throughput flat plate. According to the invention, by adopting a solid flat plate chromogenic screening technology and combining with a pH indicator, efficient screening of 3SL high-yield mutants in a large-scale mutation library is realized. According to the method, the target mutant is quickly identified by directly observing the color change on the flat plate, so that the tedious steps of strain construction, sequencing, transferring, shake-flask culture and the like in the traditional screening process are omitted, and the screening speed and efficiency are remarkably improved. Compared with the prior art, the method has the advantages that the experiment time is greatly shortened, the operation process is simplified, the requirements on human resources and equipment are reduced, and great convenience is provided for screening of the enzyme mutation library. The method has the technical advantages of rapidness, high efficiency and low cost, and is expected to be widely applied to the fields of biomedicine and food science.

A silicon carbide ohmic contact electrode structure without step etching is characterized in that an epitaxial layer different from a substrate in doping type grows on the upper surface layer of a silicon carbide substrate slice, a metal electrode array is manufactured on the surface of the epitaxial layer, and the manufacturing process of a metal electrode does not depend on tedious micro-nano manufacturing photoetching, etching and other processes; ohmic contact electrodes are separated by using an adhesive tape with uniform edge geometric morphology as a mask, and finally, a sample is uniformly cut to form a rectangular transmission line electrode without steps; based on the principle of a rectangular transmission line (TLM), preparation of the silicon carbide ohmic contact electrode which is simpler in structure and smaller in leakage current error is achieved, the problem that a traditional electrode rectangular transmission line method is complex in manufacturing process is solved, the manufacturing cost is reduced, meanwhile, the electrode preparation time is greatly shortened, and the silicon carbide ohmic contact electrode is easy to achieve.