992results about How to "Shorten experiment time" patented technology

The invention provides a full-automatic nucleic acid extraction and PCR amplification micro-fluidic chip which comprises at least one main body, wherein the main body is distributed in equal distance around the chip; the main body comprises a nucleic acid extraction unit, a PCR amplification unit, a waste liquid unit and an exhaust unit, wherein the nucleic acid extraction unit is positioned on the main body and is used for extracting, washing and eluting nucleic acid in sequence under the driving of centrifugal force and capillary force; the PCR amplification unit is adopted for PCR reaction amplification; the waste liquid unit comprises a waste liquid cavity which is used for storing a waste liquid; the exhaust unit comprises exhaust holes and a plurality of exhaust channels; and the plurality of exhaust channels are communicated with the nucleic acid extraction unit, the PCR amplification unit and the waste liquid unit and are used for exhausting air. The invention discloses a micro-fluidic chip for achieving full-automatic nucleic acid extraction and PCR reaction through centrifugal force, capillary force and siphon phenomenon, the micro-fluidic chip has no integrated equipment such as pumps or valves, so that the manufacturing difficulty and cost of the chip are greatly reduced, and moreover the reliability of the chip is improved.

The invention discloses controllable vector elimination method as well as an easy-to-use CRISPR-Cas9 tool. The invention provides a controllable vector elimination tool, which is composed of a control module and an elimination module, the control module including: a core zone which contains an elaborately-controlling promoter and a homing endonuclease encoding gene, two terminators respectively at two ends of the core zone, and homing endonuclease recognition sequences on the outer ends of the two terminators; and the elimination module including: an antibiotic resistance gene expression cassette, and homing endonuclease recognition sequences on the two ends; the tool can easily, quickly and high-effectively remove a plasmid vector in one step. A further developed easy-to-use CRISPR-Cas9 system can achieve one-step quick elimination to Cas9 and sgRNA encoding plasmids from a host cell. The easy-to-use CRISPR-Cas9 system is very convenient and practical, greatly saves experiment time, reduces experiment intensity, and is a very practical gene editing tool.

The invention relates to a multi-layer convolution feature self-adaptive fusion moving target tracking method, and belongs to the field of computer vision. The method comprises the following steps: firstly, initializing a target area in a first frame of image, and utilizing a trained deep network framework VGG-19 to extract first and fifth layers of convolution features of the target image block,and obtaining two templates through learning and training of a related filter; Secondly, extracting features of a detection sample from the prediction position and the scale size of the next frame andthe previous frame of target, and carrying out convolution on the features of the detection sample and the two templates of the previous frame to obtain a response graph of the two-layer features; calculating the weight of the obtained response graph according to an APCE measurement method, and adaptively weighting and fusing the response graph to determine the final position of the target; And after the position is determined, estimating the target optimal scale by extracting the directional gradient histogram features of the multiple scales of the target. According to the method, the targetis positioned more accurately, and the tracking precision is improved.

The invention provides a novel library construction method based on an illumina sequencing platform, and in particular provides a small-fragment DNA library construction method. Aiming at the defects of the small-fragment DNA library construction of the existing illumina sequencing platform, the purification steps can be reduced, the loss and waste of a library are reduced, the cost is lowered, and the working efficiency is improved.

The invention provides a micro fluidic chip for sorting and whole genome amplification of a single cell. The micro fluidic chip comprises a four-layer structure, wherein the four layers are stacked together in order and mutually sealed, and the structure comprises the following layers from top to bottom: a top cover, a micro valve control layer, a valve plate layer and a channel layer; and the bottom of the micro fluidic chip is externally provided with a magnet. The invention also provides a system for screening and identifying cells as well as amplification and analysis of a single cell, and the system is matched with the chip. The micro fluidic chip and the corresponding detection system can be used for sorting target cells from a large amount of cells quickly, simply and cheaply, and amplifying and analyzing whole genome DNA of a single cell therein.

The present invention provides a microfluidic chip for sorting single cells expressing EGFR and detecting multiple gene loci. The microfluidic chip includes a four-layer structure stacked together and sealed with each other, from top to bottom They are respectively a top cover, a microfluidic channel layer, a substrate with a microhole array and a bottom plate with a cavity group. The invention also provides a system for single-cell level detection of multiple mutation sites of EGFR gene matched with the chip. The microfluidic chip and the corresponding detection system of the present invention can quickly, easily and cheaply sort target cells from a large number of cells, and perform EGFR multi-gene site analysis on a single cell among them.

The invention provides an experimental device and method for estimating water-controlling and air-intake effects of a polymer. The experimental device comprises a high-pressure displacement pump, a high-pressure gas-driven unit, a confining pressure pump, a core holding unit, a measurement unit and a first controller, wherein the core holding unit is provided with an inlet end, an output end and a confining pressure hole, the high-pressure displacement pump and the high-pressure gas-driven unit are respectively communicated with the inlet end of the core holding unit, the confining pressure pump is communicated with the confining pressure hole, the measurement unit is communicated with the output end, and the first controller is connected with the measurement unit. The experimental method is used for estimating the water-controlling and air-intake effects of the polymer by virtue of gas-water relative permeability curves which are tested before and after the polymer is injected by the experimental device. According to the experimental method, a selective shielding layer, namely a polymer film, is established on the surface of a gas pool core by virtue of a technical process that the polymer is utilized for controlling yielding water of a gas well in a natural gas exploitation field, a proper polymer water control agent can be selected out by virtue of an indoor experiment, and production proposals and work instructions are provided for the gas well.

The invention relates to a water erosion experimental facility with the rotary impact of high-pressure water jet. The facility simulates the blade erosion caused by drops in a steam turbine through the collision between high-speed jet and a rotating test piece. The facility mainly comprises a motor and a motor control cabinet, a gear case, a rotor and a test piece, a high-pressure water pump and nozzle pipelines, etc. While the rotor and the test piece rotate at a high speed, the high-pressure water supplied by the high-pressure water pump can generate high-speed jet through the nozzle. And the jet hits the test piece at a speed of the vector difference between the jet and the test piece, so that hitting speed is greatly improved. Thus, the evaluation of the water erosion resistance of the steam turbine blade material within a short period on an experiment table becomes possible.

The invention aims at providing a bubble dynamics visualization research apparatus applicable to dynamic movement conditions. The bubble dynamics visualization research apparatus comprises a narrow channel experimental element, a preheater, a condenser, an air cooling tower, a water storage tank and a swinging platform, wherein a tube pass inlet of the condenser is communicated with the outlet of the narrow channel experimental element, the tube pass outlet of the condenser is communicated with the preheater, the preheater is communicated with the inlet of the narrow channel experimental element, a shell pass inlet of the condenser is communicated with the water storage tank, the shell pass outlet of the condenser is communicated with the inlet of the air cooling tower, the outlet of the air cooling tower is communicated with the water storage tank, the narrow channel experimental element is vertically fixed on the swinging platform, the preheater and the condenser are arranged on the swinging platform in parallel, a hydraulic driving mechanism is arranged below the swinging platform, a high-speed camera is arranged beside the narrow channel experiment element, and the high-speed camera is connected with a computer by virtue of a data acquisition board. The bubble dynamics visualization research apparatus provided by the invention can realize bubble behaviour research under narrow channel, natural circulation and dynamic movement conditions and has the advantages of adjustable multiple variables, quick switching of different operation conditions and high automation degree.

The invention discloses a cell chip slide for preparing microarray cell chips. The cell chip slide comprises a substrate and a sealing cover, the substrate is provided with array holes distributed in an array manner, the bottoms of the array holes are transparent and positioned on the same horizontal plane, the sealing cover is provided with hollow array posts matching with the array holes of the substrate, the array posts can extend into corresponding array holes, and the bottoms of the array posts are transparent and positioned on the same horizontal plane. The cell chip slide is simple in structure, easy to prepare and convenient to use, and can comprise various (numerous) cells arrayed in a regular array manner, therefore, detection or experimental studies of same or different indexes can be performed for various (numerous) cells simultaneously once. By the aid of the cell chip slide, operation steps are simple, and detection or experimental studies can be completed once.

The invention relates to a stereoscopic development three-dimensional experimental device of a heavy oil reservoir. The stereoscopic development three-dimensional experimental device of the heavy oil reservoir comprises a three-dimensional rock core model, a constant temperature box, a steam generator, a temperature and pressure monitoring system, constant flux pumps and an intermediate container. Vertical well connectors are arranged on an upper cover plate of the three-dimensional rock core model, and horizontal well connectors are arranged on the side face of the three-dimensional rock core model. A mixture of high-alumina cement and quartz sand is filled into an inner cavity of the three-dimensional rock core model, and the three-dimensional rock core model is further respectively provided with temperature monitoring interfaces, pressure monitoring interfaces and saturated oil holes. The first constant flux pump is connected with the steam generator, the steam generator is connected to an inlet in the lower end of a first piston container, and an outlet in the upper end of the steam generator is connected with one horizontal well connector of the three-dimensional rock core model. An outlet in the upper portion of a second piston container is connected with the intermediate container, and the intermediate container is connected to the saturated oil holes in the upper cover plate of the three-dimensional rock core model. The stereoscopic development three-dimensional experimental device of the heavy oil reservoir integrates indoor experiments of three well pattern structures of SAGD, driving and draining combination and gravity drainage assisted steam driving, the experimental well pattern structures can be switched, and convenience is provided for indoor physical simulation of stereoscopic development of heavy oil.

The invention discloses a primer system for PCR (polymerase chain reaction) identification for a deer, a pig, a cow, a sheep, a horse, a donkey, a rabbit and a chicken, and can detect eight animal species in one time so as to achieve a purpose that common animal species can be almost covered. The primer of the primer system only carries out specific amplification on a respective species target segment without reacting with other species, and therefore the primer can be suitable for multiple PCR reaction characterized in that at least two primer pairs are induced in one-time PCR reaction so as to effectively shorten experiment time. In addition, when the PCR is carried out, an optimized specific PCR reaction system and reaction program can be used, a detection flow is simplified due to the adoption of a uniform PCR detection method, a quick and efficient identification mode with the high specificity is established so as to effectively identify whether the deer blood product is true and false and identify the adulteration mode, and a modern molecular biology detection means is provided for deer blood quality control. In addition, the primer system can be cooperated with relevant reagent to be prepared into a kit, thereby being convenient to use. Meanwhile, possibility is provided for the industrial production and application, and the primer system has an excellent application prospect.

The invention discloses a natural gas hydrate sediment triaxial test device, and belongs to the field of testing the mechanical properties of natural gas hydrate sediment. The device comprises a triaxial test device main machine, a temperature control system, a pore pressure control system, a confining pressure control system and a computer data acquisition and control system. The triaxial test device main machine, which is in a dual-pressure-chamber structure, can be used for accurately measuring volume change in the sediment in a natural gas hydrate decomposing process; a load sensor is arranged inside the pressure chamber and is capable of improving the precision of intensity data; a pre-cooling hollow cavity is formed inside a base to pre-cool pore gas and pore water which enter a sample, so as to relieve the decomposition of the natural gas hydrate in a saturated water process and to improve the precision of temperature control; in a sample installation process, a quick coupling is adopted for connection, so that the operation is convenient and rapid. The device can simulate the natural gas hydrate decomposing process under mining conditions, so as to achieve the high-precision temperature control, and accurate measurement of intensity and volume change; and the device has great significances for understanding the mechanical properties of the natural gas hydrate sediment and assessing the stability of a reservoir.

The invention provides an electron gun device for generating a high-brightness femtosecond electronic impulse. The electron gun device comprises an incident window, a sealing component, a vacuum cavity, a photocathode, a grid, an anode, an observation window, a molecular pump, a pre-stage dry pump, an electronic focusing magnetic lens, an RF (Radio Frequency) electronic compression cavity, an RF signal generating device, an x-direction electronic deflector, a y-direction electronic deflector and an electronic scanning plate. Through the invention, the broadening function of the space charge effect on longitudinal pulse duration of an electronic beam impulse is effectively suppressed; the problem that the divergence of the electronic beam is increased due to electric field distortion at a small hole of the anode is solved; and the horizontal and longitudinal electronic impulses have super-high resolution factors, and the real-time detection on super-high time resolution and space resolution of ultrafast processes such as molecular reaction dynamics can be performed.

The invention provides a microfluidic chip for instantly detecting EGFR (epidermal growth factor receptor) mutation. The microfluidic chip integrates the functions of nucleic acid extraction, LAMP (loop-mediated isothermal amplification) and instant detection. The microfluidic chip solves the problem of centrifugation step required by DNA (deoxyribonucleic acid) extraction before constant-temperature DNA amplification and the problem of thermal circulation apparatus required by DNA amplification, and conforms to the general requirements for diagnostic detection in underdeveloped regions provided by WHO (World Health Organization).

The invention belongs to the technical field of oil-gas development laboratory simulation experiment facilities and evaluation, and particularly relates to a rock core gas/liquid dual-purpose permeability testing device and a reservoir damage evaluation method. The testing device at least comprises a gas-driven sampling device, a visible rock core holder and a gas flowmeter, wherein the gas-driven sampling device comprises a gas-driven gas inlet module and a gas-driven liquid inlet module; the visible rock core holder comprises an inlet end, an outlet end, a first plug body and a second plug body; and the gas flowmeter communicates with a gas outlet pipeline. The method respectively detects the rock core gas permeability K1 and rock core gas permeability K2 subjected to pollution damage by using the device, and calculates the permeability recovery ratio and permeability damage ratio. The technical scheme provided by the invention can simultaneously test the gas permeability and liquid flow permeability of the rock core as well as the permeability recovery degree of the rock core under the pollution of the working fluid. The testing device has the advantages of high simulation degree, ingenious connection, simple structure, high stability and high durability, and is convenient to operate.

The invention discloses a high-temperature creep deformation prediction method which comprises the following steps: performing short-duration test on a target sample, introducing a comprehensive parameter Q, establishing a sigma-Q relation model, and further realizing high-temperature creep deformation prediction of the target sample. The invention provides a brand new creep deformation prediction method. According to the method, change of creep deformation of a creep experiment in the solved stress along with time nearby the known stress can be predicted by utilizing the creep experiment under a certain known stress; according to the method disclosed by the invention, prediction can be performed based on a short-duration experimental result, and the experimental time in the actual experimental process is shortened; and moreover, the problem in the prior art that the test result fluctuation is large is effectively solved, due to the method disclosed by the invention, a reasonable prediction result can be obtained under the condition that the material property dispersion is large, and the accuracy and the stability of the prediction result are improved.

The invention relates to a monitoring system and an analyzing method for autonomous flying of an insect. The method comprises the following steps of: treating the insect with an experiment and putting the insect and foods into a monitoring cover; controlling the ambient temperature and humidity and the photoperiod of the monitoring cover, acquiring the video signal of the autonomous flying action of the insect in the monitoring cover by using a video acquisition device and converting the video signal into a video file; performing real-time/lag analysis on the video file by adopting an image moving target recognition technology and converting the flying action or other moving actions of the inset in the video file into a fixed quantity of original data to be stored in a text document; and sorting and analyzing the original data in the text document by defining a flying threshold value, extracting the flying parameters of the insect from the original data and evaluating the flying or active situations of the insect according to the flying parameters. By adopting the invention, the requirements on the monitoring experiment of autonomous flying or activity of the insect are met, and the experiment efficiency is improved compared with that of the prior art.

The invention discloses a multifunctional rapid detection device for detecting environment soil. The multifunctional rapid detection device comprises a shell, wherein the shell is sequentially provided with a soil sample feeding hopper, a soil grinding device and a soil flow distribution device from top to bottom; the soil flow distribution device comprises a rectangular main pipe and three rectangular secondary pipes; soil conveyor belts are arranged at the bottom ends of the three rectangular secondary pipes; a soil component detection device, a soil moisture detection device and a soil potential of hydrogen detection device are arranged below the three conveyor belts respectively; the soil component detection device comprises a near-infrared soil nutrient rapid detection instrument; a gas chromatograph is arranged at one side of the soil moisture detection device. According to the multifunctional rapid detection device provided by the invention, the potential of hydrogen of the soil, nutrients of soil solids, the moisture content of the soil and gas properties in the soil can be detected at the same time; the multifunctional rapid detection device has the advantages of completedetection functions, high detection efficiency, semiautomatic detection and labor saving and cost reducing.

The invention provides a contamination ageing test method for a silicon rubber insulator. The contamination ageing test method comprises the following steps: firstly, cutting a silicone rubber insulator string to obtain silicone rubber insulator sheets; sampling deposited ash of a to-be-detected region to obtain a dust haze sample; then mixing the dust haze sample obtained in the previous step with water to obtain a dust haze contamination solution, and carrying out manual contamination treatment on the silicone rubber insulator sheets by adopting the dust haze contamination solution to obtain to-be-detected silicone rubber insulator sheets; finally, carrying out aging test on the silicone rubber insulator sheets obtained in the previous step, and calculating to obtain a simulated aging state of the silicon rubber insulator. According to the contamination ageing test method provided by the invention, an aging effect of various complex haze environments on silicon rubber materials can be highly fitted, the experiment difficulty is simplified, the experiment cost is reduced, and the experiment time is shortened; the contamination ageing test method has an important guidance significance and economic values.

The invention provides a determination method for a conglomerate oil reservoir polymer-flooding relative permeability curve. The determination method comprises the following steps that 1, the total water cut fw of a polymer-flooding oil well is calculated; 2, a first-level linear fitting relational expression y1=ax+b is established, and fitting parameters a and b are obtained; 3, the polymer-flooding oil-water relative permeability ratios Kro/Krw under different polymer-flooding water saturations Sw are calculated according to a relational expression Kro/Krw=me<-nSw> between the relative permeability and the polymer-flooding water saturations; 4, a second-level linear fitting relational expression y2=k1x1+k2x2+k3 is established, and fitting parameters k1, k2 and k3 are obtained; 5, the oil-water two-phase relative permeability Kro and Krw under the different polymer-flooding water saturations Sw are calculated; 6, a polymer-flooding oil-water two-phase relative permeability curve is drawn. According to the determination method for the conglomerate oil reservoir polymer-flooding relative permeability curve, the more precise conglomerate oil reservoir polymer-flooding relative permeability curve can be quickly obtained, the cost is reduced, the efficiency is high, and the workload is greatly decreased.

The invention relates to a method for rapidly evaluating reliability of a semiconductor device, and belongs to the technical field of reliability evaluation. The reliability of the semiconductor device is evaluated by the aid of a parameter degradation model of the semiconductor device, and the parameter degradation model is built based on the homogeneous reaction dynamic principle according to a concentration change rule of reacting dose in the degradation process. The method solves the problem of different degradation rules of semiconductor device parameters in experiments of monotonic degradation of degradation amount with the passage of time, and non-monotonic degradation such as rise and then drop, or drop and then rise. A long-term degradation rule of the device is rapidly extrapolated by the aid of the parameter degradation model, the reliability of the device is evaluated, and experimental time is shortened.