9results about How to "Achieve cracking" patented technology

The invention belongs to the technical field of catalytic degradation, and particularly relates to a bismuth cluster / CTF composite photocatalyst and a preparation method and application thereof. The bismuth cluster and CTF are compounded through a hydrothermal method, photocatalytic cracking of lignin can be achieved, efficient separation of photo-induced electrons and holes of the photocatalyst can be achieved, fine adjustment of the electron structure of the photocatalyst is achieved by adjusting the addition amount of the bismuth cluster precursor, generation of h < + > and. O < 2-> in the photocatalytic process is improved, and the photocatalytic performance of the photocatalyst is improved. Therefore, the yield of the aromatic monomer prepared by cracking the lignin is improved, the cracking conversion rate can reach 78.5%, the yield of benzoic acid can reach 65.2%, the yield of benzaldehyde can reach 81.4%, the yield of phenyl formate can reach 52.6%, and the method is green, clean and safe.

The present invention introduces a continuous sludge extrusion device with rollers, which comprises a variable pitch stepped sludge concentrating device, a belt extrusion filter and a belt plate frame high-dryness press with rollers, wherein the outlet of the variable pitch stepped sludge concentrating device is located at the inlet of the belt extrusion filter, and the outlet of the belt extrusion filter is located at the inlet of the belt plate frame high-dryness press with rollers; the present application arranges a variable pitch stepped sludge concentrating device before the belt plate frame high-dryness press with rollers, and realizes the first progressive pressure extrusion by gradually reducing the pitch, and thereafter, a belt extrusion filter is further arranged to realize the secondary extrusion to further reduce the dryness of the sludge; finally, an upper roller extrusion assembly and a lower roller extrusion assembly are designed in the belt plate frame high-dryness press with rollers, and the upper roller extrusion assembly and the lower roller extrusion assembly are used to extrude the top of the pressing plate, thereby realizing continuous dehydration of the sludge and obtaining high-dryness sludge.

The invention provides a multiplex PCR (Polymerase Chain Reaction) micro-fluidic chip integrating electric cracking and nucleic acid purification detection and application thereof. The multiplex PCR micro-fluidic chip comprises a PDMS (Polydimethylsiloxane) layer and a glass substrate which are connected together in an ionic bonding manner, wherein the PDMS layer is internally provided with a cracking cavity, a first washing cavity, a second washing cavity and an amplification cavity which are sequentially communicated through a micro-channel at the bottom; a silver paste interdigital electrode is printed on the glass substrate through a screen printing technology, the silver paste interdigital electrode is aligned with the cracking cavity below the cracking cavity, and after a power supply is switched on, a strong electric field can be generated through current to realize electric cracking of bacteria. The chip is simple in structure, suitable for batch process manufacturing, low in cost and suitable for popularization and application. According to the present invention, the electric splitting method and the microfluidic technology are integrated, such that the splitting and the accurate quantitative detection of the bacteria are achieved, the great advantages in the detection sensitivity, the process automation, the integration level and the like are provided, and the wide application prospect in the molecular diagnosis aspect is provided.

The invention belongs to the technical field of nucleic acid extraction, and particularly relates to a large-volume sample free nucleic acid extraction kit, a nucleic acid extraction method and a detection method. The kit comprises a lysis solution, a protease K solution, a binding solution, a rinsing solution 1, a rinsing solution 2, an eluent and magnetic beads. The nucleic acid extraction method comprises the following steps: centrifuging a sample by adopting the kit, splitting and incubating; leaving the magnetic beads, and rinsing the magnetic beads with a rinsing liquid 1 and a rinsing liquid 2 for three times respectively; eluting with an eluent, and collecting to obtain a nucleic acid solution. The nucleic acid extraction method and the kit are applied to an automatic workstation, and reagents are added into different hole sites; and the program is operated after the reagent is added. The detection method comprises the following steps: determining the total amount of extracted DNA of the nucleic acid solution, the quality of extracted nucleic acid and the recovery efficiency of the nucleic acid solution. The invention provides a kit capable of being stored at normal temperature, a plurality of nucleic acid extraction methods and a detection method, can be used for free nucleic acid extraction, quality evaluation and large-scale nucleic acid extraction of a large-volume sample, and is low in detection cost and high in efficiency.

The utility model discloses a water sample enrichment and cracking device and water sample DNA monitoring equipment, when carrying out water sample DNA monitoring, first through conveying assembly conveying water sample to be measured to make water sample flow through rich crack component, and then through rich crack component filtering water sample to carry out sample enrichment, then through conveying assembly extraction cracking solution to convey cracking solution to rich crack component, and then through rich crack component to the product of enrichment carries out cracking reaction, finally through conveying assembly extraction and convey cracking product to can carry out the subsequent storage, shift and detection of cracking product, through rich crack component and conveying assembly cooperation, through the accurate control to water sample and cracking solution, realized the automatic enrichment and cracking of water sample, relative to prior art, the monitoring process is quick and convenient, and the monitoring result error is small, and the practicality is strong, is suitable for extensive promotion and application.

The invention relates to a fusarium oxysporum double-target rapid visual in-situ detection kit, a detection method and application, a crude extract of a sample obtained through physical crushing can be directly used for RAA isothermal amplification, amplification targets respectively correspond to a conserved sequence of a fusarium fungus ITS rRNA gene and a specific sequence of a fusarium oxysporum cytochrome P450 enzyme gene, and the specific sequence of the fusarium oxysporum cytochrome P450 enzyme gene corresponds to the specific sequence of the fusarium oxysporum cytochrome P450 enzyme gene. The RAA amplification product is used as a DNA target for a CRISPR / Cas12a detection system. Aiming at a conserved region of a fusarium oxysporum cytochrome P450 enzyme gene, the length of an amplified target sequence of the fusarium oxysporum cytochrome P450 enzyme gene is 120bp, and the used specific RAA primer and crRNA are respectively Fo-F / Fo-R and Fo-crRNA. The advantage of setting two detection targets and two different result reading strategies in the same onsite pathogen detection reaction is that the reliability and flexibility are enhanced, so that more accurate and timely decisions can be made under different onsite conditions.

This invention provides a biomass pyrolysis method, comprising the following steps: contacting biomass feedstock with carbon foam material, applying microwaves under an inert atmosphere to generate plasma with a temperature not lower than 3000K, causing the biomass feedstock to undergo a pyrolysis reaction; simultaneously, the volume ratio of the carbon foam material to the microwave power is 1:(25-500); wherein the unit of volume is cm³. 3 The unit of power is W. This biomass pyrolysis method has high conversion efficiency and high added value.

The utility model relates to the technical field of biomass energy conversion, disclose a kind of double fluidized bed biomass grading gasification system, comprising: pyrolysis fluidized bed carries out low-temperature pyrolysis to biomass, and by first cyclone separator, pyrolysis gas is sent into pyrolysis gas outlet, and solid matter is sent into first return feeder;Tubular reaction unit purifies the pyrolysis gas that flows;Gasification fluidized bed receives the pyrolysis gas that tubular reaction unit transports and the solid matter that first return feeder transports, after carrying out oxidation-reduction reaction, generate synthesis gas, and by second cyclone separator, synthesis gas is discharged and reused, and hot carrier in solid matter is returned pyrolysis fluidized bed by second return feeder;The gasification temperature in gasification fluidized bed is higher than the pyrolysis temperature in pyrolysis fluidized bed.The application is coupled by double fluidized bed design, realizes biomass grading pyrolysis and gasification, and tar content of synthesis gas is low;And the pyrolysis gas after purification is used as gasification agent, secondary use in gasification fluidized bed, save resources.

The invention discloses a nucleic acid real-time detection device and a micro-fluidic chip, and belongs to the field of nucleic acid detection.A cracking cavity, a uniform mixing cavity and an amplification detection cavity of the micro-fluidic chip are arranged in the radial direction, and a second lifting driving part of an electrical module is in transmission connection with a spring needle to drive the spring needle to move up and down to be connected with or separated from a lower heating module; the upper heating module and the lower heating module are used for controlling the temperature of the cracking cavity, so that a sample in the cracking cavity is cracked; the rotary driving part is in transmission connection with the lower heating module, and drives the lower heating module to rotate, so that liquid in a reagent cavity and a cracking cavity in a micro-fluidic chip positioned on the lower heating module enters the uniform mixing cavity, and liquid in the uniform mixing cavity enters the amplification detection cavity; by means of the design, centrifugal force is adopted to drive liquid, an external pump and an external valve are not needed, the size of the detection device is reduced, miniaturization is facilitated, carrying is convenient, and instant detection can be conducted.