43results about How to "Convenient purification work" patented technology

The invention relates to a novel long-acting glucagons like peptide-1 (GLP-1) analog and a synthesis method thereof. The synthesis method comprises the following steps: modifying the 8th, 23rd or 37th site of the natural GLP-1 to obtain the GLP-1 analog having longer pharmacological action time; quickly synthesizing a peptide chain by adopting a microwave-promoted solid-phase synthesis method; reacting cysteine residues with a 4-hydroxyl coumarin analog to obtain a target polypeptide; purifying the crude product; and carrying out freeze-drying to obtain the GLP-1 analog.

The invention relates to long-acting glucagon-like peptide 1 (GLP-1) analogues and a synthesis method thereof. GLP-1 analogues with longer pharmacological action time are obtained through adding a modified 37th amino acid to natural GLP-1, the synthesis of target polypeptides is quickly realized through a microwave-promoted solid-phase synthesis method, and crude products are purified and freeze-dried to obtain the GLP-1 analogues.

The invention relates to a long-acting Exendin-4 analogue and a synthesis method thereof. Exendin-4 is transformed to obtain the Exendin-4 analogue having longer pharmacological action time, synthesis of a target polypeptide is rapidly achieved by a microwave-promoted solid phase synthesis method, and a crude product is purified and freeze-dried to obtain the Exendin-4 analogue.

The invention relates to glucagon-like peptide 1 (GLP-1) analogues with a long-acting effect and a synthesis method thereof. GLP-1 analogues with longer pharmacological action time are obtained through replacing/modifying 17th, 26th, 34th and 37th amino acids of natural GLP-1, the synthesis of target polypeptides is quickly realized through a microwave-promoted solid-phase synthesis method, and crude products are purified and freeze-dried to obtain the GLP-1 analogues.

A reagent kit for detecting cell wither features that its detecting protein is Annexin B1 instead of current Annexin V, resulting in high expression in colibacillus. Its other advantages are high speed and high sensitivity.

The invention relates to novel long-acting glucagon-like peptide 1 (GLP-1) analogues and a synthesis method thereof. GLP-1 analogues with longer pharmacological action time are obtained through replacing/modifying 17th, 26th, 34th and 37th amino acids of natural GLP-1, the synthesis of target polypeptides is quickly realized through a microwave-promoted solid-phase synthesis method, and crude products are purified and freeze-dried to the GLP-1 analogues.

The invention discloses a production method for Pichia pastoris expression recombinant human interleukin 11, which comprises the step of combined purification sequentially through reverse phase column chromatography, hydrophobic column chromatography and gel column chromatography. By adopting the production method, high-purity medicinal recombinant human interleukin 11 proteins can be prepared ina large scale.

The invention relates to new bitter gourd MC-JJ2162 peptide analogs capable of efficiently regulating blood sugar level, and a method for solid phase synthesis of bitter gourd MC-JJ2162 peptide analogs under microwave irradiation. The method comprises the following steps: obtaining the MC-JJ2162 peptide analogs having longer pharmacological effect time by modifying 2, 4, 5, 8 or 9 site of natural bitter gourd analogs; efficiently and quickly realizing the chemical synthesis of the MC-JJ2162 peptide analogs by adopting a solid phase synthesis method under microwave irradiation; purifying the crude products by using preparation grade highly effective liquid phase; and freeze-drying the purified products to obtain the MC-JJ2162 peptide analogs.

The invention relates to new bitter gourd MC-JJ6 peptide analogs capable of efficiently regulating blood sugar level, and a method for solid phase synthesis of bitter gourd MC-JJ6 peptide analogs under microwave irradiation. The method comprises the following steps: obtaining the MC-JJ6 peptide analogs having longer pharmacological effect time by modifying 2, 4, 5, 8 or 9 site of natural bitter gourd analogs; efficiently and quickly realizing the chemical synthesis of the MC-JJ6 peptide analogs by adopting a solid phase synthesis method under microwave irradiation; purifying the crude products by using preparation grade highly effective liquid phase; and freeze-drying the purified products to obtain the MC-JJ6 peptide analogs.

The invention relates to a group of Gateway eukaryotic expression system for stably and efficiently expressing active fusion proteins. The system comprises two universal destination vectors, namely, a 12xHis-GFP-GW (vector A) and a 12xHis-GW-GFP (vector B), and a user can select the two universal destination vectors to carry out LR reaction with an ENTR vector according to needs, so that a final expression destination vector can be obtained. According to the two universal vectors, a pK7WGF2 vector or a pK7FWG2 vector is used as an initial skeleton, and fusion and insertion are respectively carried out on a coding sequence of 12xHis by using restriction enzyme SpeI and recombinase MultiS. The invention provides a convenient and fast molecular cloning system as a necessary supplement for the previous patent (patent application number 202110472170. X), which is used for efficiently and accurately expressing a small number of eukaryotic proteins which cannot be expressed in a prokaryotic system, so that a protein expression and purification system is more comprehensive and perfect, has more powerful functions, and can powerfully promote the purification work of active proteins, and a basis is established for functional research of the proteins.

The invention relates to novel tag protein for protein enrichment expression and intracellular localization as well as application thereof. The tag protein is derived from membrane protein-reflectin A1(RA1) of a squid iridophore. The application of the novel tag protein for protein enrichment expression and intracellular localization comprises the following steps: (a) providing a construction object, wherein in an open reading frame of a gene expression vector, the construction object, from 5' to 3' segments, sequentially comprises the following operable connecting components: an encoding geneof eGFP and an encoding gene of the RA1; (b) transferring the construction object in (a) into a host cell so as to express eGFP and RA1 tags; (c) providing a group of recombinant protein formed by the eGFP and RA1 tags, wherein the recombinant protein contains functional areas of the eGFP and the RA1, and the eGFP exerts under the action of the RA1 functional area; and (d) performing intracellular enrichment and spatial orientation to be favorable for the subsequent separation and purification.

The invention relates to a novel high-efficiency hypoglycemic balsam pear MC-JJ0104 polypeptide analogue and a microwave-accelerated solid-phase synthesis method. The MC-JJ0104 polypeptide analogue with longer pharmacological action time is obtained by modifying the 3, 4, 8 or 9 locus of a natural balsam pear polypeptide. The chemical synthesis of the polypeptide analogue is efficiently and quickly realized by the microwave-accelerated solid-phase synthesis method. The crude product is purified by a preparative scale high-efficiency liquid phase, and the MC-JJ0104 polypeptide analogue is finally obtained through freeze-drying.

The invention relates to new bitter gourd MC-JJ6222 peptide analogs capable of efficiently regulating blood sugar level, and a method for solid phase synthesis of bitter gourd MC-JJ6222 peptide analogs under microwave irradiation. The method comprises the following steps: obtaining the MC-JJ6222 peptide analogs having longer pharmacological effect time by modifying 2, 4, 5, 8 or 9 site of natural bitter gourd analogs; efficiently and quickly realizing the chemical synthesis of the MC-JJ6222 peptide analogs by adopting a solid phase synthesis method under microwave irradiation; purifying the crude products by using preparation grade highly effective liquid phase; and freeze-drying the purified products to obtain the MC-JJ6222 peptide analogs.

The invention relates to a novel efficient hypoglycemic balsam pear MC-JJ0108 polypeptide analogue and a microwave irradiation solid phase synthesis method thereof. Locus 1,3,4,7 or 8 of natural balsam pear polypeptide is modified so as to obtain the MC-JJ0108 polypeptide analogue with longer pharmacological action time; and chemical synthesis is realized efficiently and rapidly by a microwave irradiation solid phase synthesis method. A crude product is subject to high-performance liquid purification and is freeze-dried so as to obtain the MC-JJ0108 polypeptide analogue.

The invention discloses a method for producing Pichia anomala expression recombination human interleukin 11 which consists of reversed phase column chromatography, hydrophobic column chromatography, gel column chromatography sequent combination purification. The method can be applied for mass preparation of high purity medicinal recombination human interleukin 11 proteins.

The invention relates to novel high-efficiency blood sugar-reducing balsam pear MC-JJ2163 polypeptide analogs and a microwave-enhanced solid-phase synthesis method thereof. The MC-JJ2163 polypeptide analogs with longer pharmacological action time are obtained by modifying 2, 4, 5, 8 or 9 position of natural balsam pear polypeptides. The chemical synthesis of the balsam pear MC-JJ2163 polypeptide analogs is realized efficiently and quickly through the microwave-enhanced solid-phase synthesis method; coarse products are purified through a preparative high performance liquid phase; and finally the MC-JJ2163 polypeptide analogs are obtained by freeze-drying.

The invention discloses a subunit influenza vaccine split agent and application thereof, and belongs to the technical field of vaccine production. The subunit influenza vaccine split agent comprises nonoxynol-9 with a mass concentration of 0.1-5%, sodium hydroxide with a mass concentration of 1-5 g/L, sodium dihydrogen phosphate with a mass concentration of 5-20 g/L, sucrose with a mass concentration of 10-60% and the balance distilled water. According to the subunit influenza vaccine split agent and application thereof, the split agent is subjected to a density gradient centrifugation process, due to the fact that the split agent with the formula has the character of efficiently splitting influenza viruses, virus particles do not need to be split in advance, and are split in the centrifugation process instead, that is to say, one-step splitting is achieved, thus a purification treatment process is shortened, no obvious M protein bands or N protein bands appear in a product PAGE electrophoresis, and the purity of a main antigen component HA of a vaccine is higher than 90%, and is higher than those of products which are obtained by adopting aforehand splitting and other split agentformulas.