Method for solid phase synthesis of bitter gourd MC-JJ2162 peptide analogs under microwave irradiation and application of bitter gourd MC-JJ2162 peptide analogs

Abstract

The invention relates to new bitter gourd MC-JJ2162 peptide analogs capable of efficiently regulating blood sugar level, and a method for solid phase synthesis of bitter gourd MC-JJ2162 peptide analogs under microwave irradiation. The method comprises the following steps: obtaining the MC-JJ2162 peptide analogs having longer pharmacological effect time by modifying 2, 4, 5, 8 or 9 site of natural bitter gourd analogs; efficiently and quickly realizing the chemical synthesis of the MC-JJ2162 peptide analogs by adopting a solid phase synthesis method under microwave irradiation; purifying the crude products by using preparation grade highly effective liquid phase; and freeze-drying the purified products to obtain the MC-JJ2162 peptide analogs.

Description

technical field [0001] The invention relates to a MC-JJ2162 polypeptide analog and a microwave-promoted solid-phase synthesis method thereof. Background technique [0002] Diabetes is an important disease that endangers human health today. At present, about 5%-7% of people in the world suffer from diabetes. It is considered to be the third largest killer of human disability and death after cardiovascular and cerebrovascular diseases and malignant tumors. . [0003] Diabetes is divided into type I and type II. Type I diabetes is an autoimmune disease that requires insulin injections for survival. Type II diabetes accounts for about 95% of the total number of diabetic patients. Type II diabetes is caused by metabolic disorders of islet cells, insufficient insulin secretion, or insulin resistance, which is a metabolic disease caused by insulin resistance mainly accompanied by relatively insufficient insulin secretion, or relatively insufficient insulin secretion accompanied b...

AI Technical Summary

Problems solved by technology

Using the traditional solid-phase method to synthesize long peptides, the yield is often very low and the impurities are more difficult to purify, thus losing the meaning of synthesis from a practical point of view

Secondly, the synthesis cycle is long. In the case of no difficult peptide sequence in the middle, it usually takes nearly a week to complete the synthesis cycle of 30 peptides. If there is a difficult peptide sequence, it is often impossible to synthesize the target peptide.