19488 results about "Freeze dry" patented technology

A freeze dried biocompatible matrix comprising plasma proteins, useful as implants for tissue engineering as well as in biotechnology, and methods of producing the matrix are provided. Mechanical and physical parameters can be controlled by use of auxiliary components or additives which may be removed after the matrix is formed in order to improve the biological properties of the matrix. The matrices according to the present invention may be used clinically per se, or as a cell-bearing implant.

In a method of making an absorbent composite, a porous, stabilized structure is formed and impregnated with a flowable superabsorbent precursor. The flowable superabsorbent precursor is cross-linked to form a superabsorbent material within the stabilized structure. The surface area of one of the flowable superabsorbent precursor impregnated with the stabilized structure and the superabsorbent material formed within the structure is increased. In one embodiment, the surface is increased by freeze drying the impregnated structure.

A novel method of forming catalyst particles, on which carbon nanotubes grow based, on a substrate with increased uniformity, and a method of synthesizing carbon nanotubes having improved uniformity are provided. A catalytic metal precursor solution is applied to a substrate. The applied catalytic metal precursor solution is freeze-dried, and then reduced to catalytic metal. The method of forming catalyst particles can minimize agglomeration and / or recrystallization of catalyst particles when forming the catalyst particles by freeze-drying the catalyst metal precursor solution. The catalyst particles formed by the method has a very uniform particle size and are very uniformly distributed on the substrate.

The invention discloses a preparation method of plant enzyme food, belonging to the field of food processing technology. The method comprises the following steps of: performing twice fermentation and compounding on the natural plant raw materials of fresh fruits, vegetables, mushrooms, medicines, grains and flowers to obtain the secondary-fermentation stock solution or raw sauce, and storing; filling and sealing the secondary-fermentation stock solution or raw sauce to obtain liquid or sauce-type plant enzyme food or compound plant enzyme food; or performing low-temperature concentration and freeze drying to obtain solid plant enzyme food or compound plant enzyme food. Due the adoption of the preparation method, the obtained natural plant enzyme food has the functions of improving the immunity of human body, activating cells, promoting metabolism and the like.

A method of processing an acellular tissue matrix to give a particulate acellular tissue matrix includes: cutting sheets of dry acellular tissue matrix into strips; cryofracturing the dry acellular tissue matrix strips at cryogenic temperatures; separating the resulting particles by size at cryogenic temperatures; and freeze drying the fraction of particles desired size to remove any moisture that may have been absorbed to give a dry particulate acellular tissue matrix. Rehydration of the dry particulate acellular tissue matrix may take place just prior to use. The particulate acellular tissue may be applied to a recipient site, by way of injection, spraying, layering, packing, in-casing or combinations thereof. The particulate acellular tissue may further include growth and stimulating agents selected from epidermal growth factor, fibroblast growth factor, nerve growth factor, keratinocyte growth factor, platelet derived growth factor, vasoactive intestinal peptide, stem cell factor, bone morphogetic proteins, chondrocyte growth factor and combinations thereof. Other pharmaceutically active compounds may be combined with the rehydrated particulate material including: analgesic drugs; hemostatic drugs; antibiotic drugs; local anesthetics and the like to enhance the acceptance of the implanted particulate material. The particulate material product may also be combined with stem cells selected from mesenchymal stem cells, epidermal stem cells, cartilage stem cells, hematopoietic stem cells and combinations thereof.

A lyophilization process which comprises dissolving a material in one or more solvents for said material to form a solution; forcing said material at least partially out of solution by combining the solution and a non-solvent for the material, which non-solvent is miscible with the solvent or solvents used and wherein said non-solvent is volatilizable under freeze-drying conditions. In addition, for hydrophobic and / or lipophilic materials, the anti-solvent can be omitted, and the solution of the material in the solvent can be subjected directly to freeze drying. The lyophilizates can then be reconstituted with typical aqueous diluent in the case of hydrophilic materials. Hydrophobic and or lipophilic materials can be initially reconstituted with propylene glycol and / or polyethyleneglycol to form a high concentration solution therein and this is further diluted for use with a diluent of Intralipid, plasma, serum, or even whole blood.

Freeze-dried disintegrating tablets, said tablets containing at least a therapeutic agent, a matrix forming agent and a binding agent, in which the tablets contain more than 20% by weight of a matrix forming agent selected from the group consisting of maltodextrins having a DE value between 12 and 40, isomalt and mixtures thereof, the weight ratio between said matrix forming agent and the binding agent being comprised between 2:1 and 50:1.

The present invention provides freeze-dried compositions that include an extract of human feces and a cryoprotectant, and methods for making and using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and / or iatrogenic condition of the colon.

A readily-soluble freeze-dried solid preparation of hGH with a minimal content of degradation products in terms of deamidation, dimers, polymers, and sulphoxide forms, obtainable by a method comprising a single lyophilization of an aqueous slurry of an amorphous hGH isoprecipitate, the slurry having a pH of from about 4.7 to 5.0 and being essentially free of buffer components other than acetate.

A composition comprising a collagen protein and demineralized bone matrix is described wherein the composition is chemically cross-linked with a carbodiimide such as N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC). The crosslinking reaction can be conducted in the presence of N-hydroxysuccinimide (NHS). The collagen can be in a porous matrix or scaffolding. The DBM can be in the form of particles dispersed in the collagen. A method of making the composition is also described wherein a collagen slurry is cast into the desired shape, freeze dried to form a porous scaffolding and infitrated with a solution comprising the cross-linking agent. The composition can be used as an implant for tissue (e.g., soft tissue or bone) engineering.

Tissue repair compositions, particularly bone repair compositions, containing demineralized bone fragments and homogenized connective tissues. The compositions can be used in the form of an injectable gel, an injectable paste, a paste, a putty, or a rehydratable freeze-dried form.

The invention relates to microcapsules, and a continuous micro-encapsulation water-in-oil-in-water microencapsulation process through in situ and interfacial polymerization of the emulsion. The formulation comprises a continuous water phase having a dispersion of microcapsules which contain oil drops and wherein the inside of each oil phase drop -containing optionally oil-soluble materials- there is a dispersion of water, or aqueous extract or water dispersible material or water soluble material. The oil drops are encapsulated with a polymerisable material of natural origin. Such microcapsules are appropriated for spray-dry processes, to be used as dry powder, lyophilised, self-emulsifiable powder, gel, cream and any liquid form. The active compounds included in te microcapsules are beneficial to the health and other biological purposes. Such formulations are appropriate to be incorporated in any class of food, specially for the production of nutraceuticals, as well as cosmetic products (such as rejuvenescence creams, anti-wrinkle creams, gels, bath and shower consumable products and sprays). The preparations are adequate to stabilise compounds added to the food, media for cultivating microbes and nutraceuticals, specially those which are easily degradable or oxidizable.

Polymer and drug containing compositions and method of preparing such compositions are disclosed. The dispersed phase formulation has a polymer, a pharmaceutically or biologically active agent and a small fraction of low pKa acid additive. Stable, filter sterilizable, non-gelling solutions containing GnRH analogues at least at levels typically used in sustained release formulations and a method of increasing solubility of a high level of a GnRH analogue or a freeze-dried antgonist of GnRH in a polymer containing solution are also disclosed. The amount of the acid additive in the polymer solution is such that it is sufficient to increase the solubility of the high level of the GnRH analogue in the polymer solution without affecting the release characteristics of the microspheres prepared therefrom.

The invention relates to a method for preparing graphene powder in large scale, which is characterized by comprising the following steps of: firstly, uniformly peeling graphene oxide into a graphene oxide suspension solution; then, atomizing the graphene oxide solution by using the spray drying technology comprising spray pyrolysis drying and spray freeze drying, and removing a solvent to obtain graphene oxide powder; and finally, oxidizing grapheme by using the non-expansion heat treatment process to obtain non-agglomerative graphene powder. The continuous preparation process of the spray technology and the non-expansion heat treatment process ensure the large-scale preparation of the graphene powder. The prepared graphene powder comprising intermediate product graphene oxide powder does not have agglomeration and has good dispersivity in the solvent. The graphene powder is used as a filling material to prepare high strength composite materials, conductive composite materials, novel air-tight flame-retardant composite materials, novel nanodevices and the like.

The present invention discloses a natural fruit and vegetable ferment beverage, and a preparation method thereof. The preparation method for the ferment beverage comprises: adding freshly-squeezed fruit and vegetable juice to marine fish skin collagen peptide; adopting the resulting mixture as a fermentation substrate; carrying out dilution, slurry mixing and sterilization, and then adopting a direct vat and freeze-dried type lactic acid starter to carry out fermentation; stopping the fermentation after 7-11 days until the pH value is 4.3; adding a supplementary feed and carrying out sterilization; then adding high-activity dried yeast to carry out fermentation; stopping the fermentation after 5-7 days until the pH value is 4.0; after storing the fermentation broth at the low temperature, carrying out homogenization, concentration, centrifugation, blending, sterilization and bulking to finally prepare the novel natural fruit and vegetable ferment beverage. According to the ferment beverage of the present invention, the stepwise fermentation is adopted for the freshly-squeezed fruit and vegetable juice by using the lactic acid bacteria and the yeast; the marine fish skin collagen peptide is added to promote the growth and the metabolism of fermentation bacteria, such that metabolic products of nutritional ingredients in the natural fruits and vegetables are rich so as to provide a beneficial nutrition and health effect.

The composition and method for healing tissues is a medicinal composition for facilitating the growth, protection and healing of tissues and cells in animals and humans. The composition is formulated as a either a powder, gel, paste, film, fluid injectable, rehydratable freeze-dried paste or sponge, sprayable solution, topically applied patch with adhesive and reservoir system, an intermediate for coatables such as films and bandages, a matrix for membranes, or as a matrix of flexible polymer(s), or delivered as either an orally ingestible liquid, tablet or capsule. The main ingredient of the formulated compositions is hydrolyzed collagen, which can be combined with polysulfated glycosaminoglycans, hyaluronic acid or salts thereof, or a glucosamine salt, and mixtures thereof. The composition may be formulated as an aqueous eye drop solution.

The invention is directed to a process for making a tissue repair implant having a porous sponge-like structure to repair bone, cartilage, or soft tissue defects by producing a connective tissue homogenate from one or more connective tissues; mixing the connective tissue homogenate with a carrier solution to produce a connective tissue carrier; optionally mixing one or more natural or synthetic bone fragements with said connective tissue carrier to produce a tissue repair mixture; freezing or freeze-drying the tissue repair mixture to produce a porous sponge-like structure and create a three-dimensional framework to entrap the natural or synthetic bone fragments, treating the frozen or freeze-dried porous sponge-like structure with one or more treatment solutions to produce a stabilized porous sponge-like structure. A crudely fragmented connective tissue from one or more connective tissues is optionally mixed with the tissue repair mixture before freezing or freeze-drying. The tissue repair implant having a porous sponge-like structure is optionally combined with one or more bioactive supplements or one or more agents that have bioactive supplement binding site(s) to increase the affinity of growth factors, differentiation factor, cytokines, or anti-inflammatory agents to the tissue repair implant. The invention is further directed toward applying such tissue repair implant for tissue repair.

The invention relates to a kit for measuring the D-Dimer content by adopting a latex immunonephelometry method. The kit comprises a D-Dimer reagent R1, a D-Dimer reagent R2, a D-Dimer diluent and a D-Dimer calibration product, wherein the D-Dimer reagent R1 comprises a buffering solution, a stabilizing agent (1), coagulant and preservative; the D-Dimer reagent R2 comprises mouse anti-human D-Dimer monoclonal antibody latex enhanced particles, a buffering solution, a stabilizing agent (2) and preservative; the D-Dimer diluent comprises a buffering solution and preservative; and the D-Dimer calibration product is prepared by subpacking and freeze-drying a solution comprising a fibrin degradation product D-Dimer, a buffering solution, a stabilizing agent (3), excipient and preservative. The kit has the advantages of simple and rapid operation, accurate quantification, high sensitivity, strong specificity, low detection cost and strong instrument compatibility and is suitable for being popularized and used in various big-scale and small-scale hospitals.

The present invention comprises the discovery and development of an effective cryoprotectant composition, without containing skim milk or any other animal-derived compounds, to achieve long-term stability of freeze-dried lactic acid bacteria (LAB), at different temperatures, whereby the retention of viability of the freeze-dried LAB after 6 months of storage, preferably after 9 months of storage, more preferably after 12 months of storage is more than 50%. The invention is in the field of producing freeze dried bacteria, in particular Lactic acid bacteria. More in particular, the invention relates to the use of a novel combination of cryoprotectants for increasing the viability of bacteria after freeze drying, improving the texture of the lyofilized cake for easy grinding and improving the long term stability of the freeze dried bacteria at different temperature conditions. The invention further relates to such freeze dried bacteria for use in food industry or in human or animal health applications. More in particular, the invention relates to the increased viability and long-term storage of recombinant bacteria capable of expressing heterologous proteins or peptides and administered to humans or animals for therapeutic or vaccination purposes.

This invention provides methods and compositions to preserve bioactive materials in a dried foam matrix. Methods provide non-boiling foam generation and penetration of preservative agents at temperatures near the phase transition temperature of the membranes.

The invention provides a method for preparing fibrilia carboxylation cellulose nanowhiskers, which is characterized by comprising the following steps: soaking fibrilia powder in sodium hydroxide for processing, then processing the fibrilia powder by a former treating agent and taking out the fibrilia powder to be dried in a vacuum oven to obtain preprocessed fibrilia powder; and placing the preprocessed fibrilia powder in a TEMPO oxidation system for catalytic oxidation to obtain a stable cellulose nanowhiskers suspending liquid after mechanical processing and freeze drying the suspending liquid to obtain the fibrilia carboxylation cellulose nanowhiskers having grain diameters of 3-10 nm. According to the invention, fibrilia carboxylation and nano fibrillation are realized and surfaces of prepared nanocrystalline celluloses have carboxyl functional groups, thus the surfaces generate negative charges, electrostatic repulsion among the negative charges can avoid the reunion of nanoparticles, so that the nanocrystalline celluloses can be well dispersed in water and the obtained nanocrystalline celluloses have excellent uniformity of grain sizes.

The invention relates to a lactobacillus microcapsule, a method for preparing the same, and the use of the same. The lactobacillus microcapsule consists of an outer layer of wall material, a freeze dried protective agent and lactobacilli. The invention also provides the method for preparing the lactobacillus microcapsule and the use of the lactobacillus microcapsule as a feed additive. The lactobacillus microcapsule can effectively protect the lactobacilli in a core material, prolong the survival time of the lactobacilli at room temperature and improve the tolerance of the lactobacilli to the metal ions in the feed; in addition, the lactobacillus microcapsule has good gastric acid resistance and can disintegrate in the intestinal canal quickly to release the lactobacilli, thereby improving the utilization rate of the lactobacilli, balancing the micro-ecological environment in the intestinal canal, suppressing the growth of pathogenic bacteria, protecting the health of the intestinal canals of animals, reducing the incidence rate of the intestinal canals of the animals and the like.

The present invention relates to novel compositions of freeze-dried ginseng berry and other natural health promoting ingredients in a mixture suitable for making tea. The compositions and methods of preferred embodiments of the present invention provide a natural vitamin containing composition for making a tea beverage which can be consumed hot or cold and which provides the consumer with a refreshing, delicious, stimulating and healthful experience.

The composite spongy biological dressing contains chitosan, collagen and calcium alginate with weigh tmixing ratio o f 0.5-8:0.5-8:0.1-8. Its preparation method includes the following steps: selecting chitosan and collagen type I, adding calcium alginate, compounding and cross-linking, using buffer solution to make neutralization, emulsifying, prefreezing and one-step freeze-drying so as to obtain the invented dressing with good biological compatibility and strong adhesion property. Said invented dressing possesses active function of promoting wound healing and hemostatic action, can be combined with anti-bacterial medicine to obtain gene engineeirng dressing for curing wound surface infection, also can be combined with active growth factor or active cell to form gene engineering dressingfor curing intractable ulcer and burn wound surface.

The invention discloses a plant polysaccharide cigarette filter tip and a preparation method thereof. Plant polysaccharide, starch, adsorbent, plasticizer, emulsifier, waterproofing agent and plant protein are blended according to a certain proportion, so that the filter tip is prepared. The preparation method includes the following steps: (a) the starch, water and the plasticizer are blended and stirred, so that modified starch solution is obtained; (b) the plant polysaccharide and the modified starch solution are blended; (c) under certain conditions, the emulsifier, the waterproofing agent and the adsorbent are added into the mixture of the plant polysaccharide and the modified starch solution, and after stirring, the mixture is injected into a long cylindrical mold made of anti-freezing, heat-proofing material and molded; (d) the semifinished product is placed into a freezing environment and frozen, i.e. prefreezing; (e) the semifinished product in step d is placed into a freeze dryer and freeze-dried; (f) the porous composite body is taken out of the mold and deburred, and thereby a filter tip stick is obtained. Material resources are rich, the cost is low, and the three wastes cannot be produced. The performance is good, the filter tip can reduce the nicotine content by 40.2 to 84.1 percent, the tar by 26 to 72.1 percent and the nicotinamide by 20 to 39.2 percent, and after use, waste can be biodegraded.

Techniques, mixtures, mixing and delivery kits, and improved delivery instruments for implantation of micronized allograft tissue over a microfractured defect. Allograft cartilage tissue is delivered over a cartilage defect that has been debrided and microfractured, without the need for a periosteal covering or separate type of patch sewn over the top. The allograft tissue may be any micronized cartilage particulates obtained by various methods, for example, cartilage delivered in its native form, dehydrated via lyophilization, “freeze-dried,” dehydrated via desiccation, or dehydrated by any other method.

The invention discloses a biomedical material and a product thereof. The biomedical material has main characteristic that a compound having a catechol group structure is grafted on chitosan to form a chitosan biomedical material having the catechol group structure; or the grafted chitosan is cross-linked to prepare a novel macromolecular biomedical material. The catechol has cell adhesion, so that tissue adhesiveness of the chitosan can be changed, and blood cells can be adsorbed on the material surface to achieve better bleeding-steeping effect. By virtue of the mediation, performance of the chitosan is improved, and tissue affinity is improved. The material is prepared into a novel bleeding-stopping sponge by freeze-drying, and prepared into novel dressing by spinning or prepared into powder dressing by reverse flocculating.

A vessel receives a liquid material, which is freeze-dried in situ within the vessel. A gas permeable material carried by the vessel provides gas transport for sublimation during drying. After freeze drying, an oxygen-free inert gas is introduced into the vessel through the gas permeable material, to occupy the interior of the vessel with the freeze-dried material. The gas permeable material is covered, to trap the oxygen-free inert gas within the vessel with the freeze-dried material. The freeze-dried material is stored in the entrapped oxygen-free inert gas within the vessel for a storage period. The vessel receives a reconstituting liquid for mixing with the freeze-dried material, and conveys the reconstituted freeze-dried material from the vessel for administration to an individual.

The invention relates to a drink, in particular to a probiotic type fruit and vegetable enzyme solid drink. The probiotic type fruit and vegetable enzyme solid drink is prepared from raw materials in parts by mass as follows: 10-50 parts of prebiotics, 5-40 parts of water-soluble dietary fiber, 0-20 parts of sugar alcohol, 0.1-15 parts of fruit powder, 0.1-10 parts of fruit and vegetable enzyme powder, 0.01-15 parts of freeze-dried bifidobacterium infantis powder, 0.01-20 parts of freeze-dried bifidobacterium breve powder, 0.01-30 parts of freeze-dried Lactobacillus plantarum powder, 0.01-25 parts of freeze-dried bifidobacterium longum powder and 0.01-20 parts of freeze-dried Lactobacillus casei powder. The probiotic type fruit and vegetable enzyme solid drink adopts the natural raw materials and the scientific formula and is balanced in nutrition and beneficial to intestinal health and body immunity.