3437 results about "Freeze dry" patented technology

The invention provides a probiotics plant solid beverage for treating hyperuricemia and gout. The solid beverage is prepared by mixing freeze-dried probiotics powder and plant extract with the addition of proper auxiliary materials. The solid beverage comprises the following components: the plant extract, the auxiliary materials and the probiotics, with the sum of the mass percentage of each component being 100%. The plant extract comprises celery seed powder, sophora flower bud powder and platycodon root powder. The invention has an obvious effect on lowering uric acid and relieving gout.

The invention relates to the technical field of hippocampus extract, in particular to a hippocampus-sourced neuroprotection and anti-oxidative stress polypeptide and a preparation method thereof.The amino acid sequence of the polypeptide is shown as SEQ ID No.1. The preparation method comprises the following steps that 1, 500 g of fresh hippocampus is weighed, surface silt is removed, freeze-drying is conducted at the temperature of-60 DEG C, then the hippocampus is smashed through a smashing machine and sieved through a 20-mesh sieve, and the hippocampus extract is obtained; dialyzing with distilled water for 24 hours, and freeze-drying to obtain hippocampus powder; (2) weighing 200g of hippocampus powder, adding 1000ml of 95% ethanol, extracting for three times, removing fat-soluble substances, and extracting and freeze-drying precipitates by using a 1 mol / L NaCl solution under the condition of 4 DEG C in combination with ultrasonic assistance to obtain crude hippocampus protein; (3) the hippocampus crude protein is taken, the hippocampus polypeptide is prepared through an ultrasonic-assisted bionic digestion method, and the hippocampus polypeptide has the advantages of being specific in molecular structure, clear in action mechanism, stable in gastrointestinal tracts, capable of protecting nerves, resistant to oxidative stress and the like.

The invention discloses a kit for detecting tomato brown crinkled fruit virus based on enzyme-mediated dual-amplification nucleic acid amplification and a detection method. The detection system comprises a primer pair DNA (deoxyribonucleic acid) upstream primer (F5) and a DNA downstream primer (R6) for amplifying the tomato brown crinkled fruit virus movement protein gene, a probe (RNA primer (RNA1)) for detecting the MP gene, a nucleic acid amplification group enzyme system (recombinase, single-chain binding protein, polymerase and ATP (adenosine triphosphate) energy regeneration enzyme), a signal amplification group enzyme system (positioning enzyme and cutting enzyme), activating enzyme NTP, a buffer solution and RNase-free Water. The system composed of the freeze-drying protective agent is prepared into the pre-subpackaged freeze-dried balls. The kit disclosed by the invention can realize DNA detection of aM concentration, and besides rapid qualitative detection of the tomato brown crinkled fruit virus, early screening of the tomato brown crinkled fruit virus can also be realized due to high detection sensitivity.

The present invention provides a Lacticaseibacillus rhamnosus UP180, and its preservation number is CGMCC NO.32109. The present invention also provides a fermentation product obtained by fermenting astragalus extract by the Lacticaseibacillus rhamnosus UP180. The present invention also provides a probiotic preparation, including the Lacticaseibacillus rhamnosus UP180, which helps to promote growth and development. The present invention also provides a preparation method of the probiotic preparation, including the steps of fermenting and culturing the probiotics to obtain bacterial mud, and freeze-drying the bacterial mud after mixing it with a freeze-drying protective agent. It is convenient for subsequent industrial applications.

The invention discloses preparation and application of antioxidative, anti-inflammatory and hypoglycemic lactoferrin peptide, and belongs to the field of food science and nutriology. According to the invention, lactoferrin is used as a raw material, hydrolysis is carried out by using a composite enzymatic hydrolysis method, and the molecular weight is obtained by ultrafiltration; the preparation method comprises the following steps: preparing a component of antioxidant, anti-inflammatory and hypoglycemic peptide from the active peptide YLSW (Tyr-Leu-Ser-Trp), carrying out freeze drying on the component to obtain peptide powder rich in antioxidant, anti-inflammatory and hypoglycemic peptides, identifying peptide fragments contained in the peptide powder by utilizing an LC-MS / MS technology, and screening out the active peptide YLSW (Tyr-Leu-Ser-Trp) with antioxidant, anti-inflammatory and hypoglycemic triple functions. The active peptide is added into the product to obtain the antioxidant, anti-inflammatory and hypoglycemic functional product.

The invention provides a collagen freeze-dried product and a preparation method thereof, and belongs to the technical field of biology. In the formula design, through the synergistic effect of the arginine (salt) and the surfactant, denaturation and aggregation of the collagen freeze-dried product in the production process and the storage period are inhibited, the stability of the product is improved, and the prepared collagen freeze-dried product is high in activity, purity and safety. In the freeze-drying process, pre-freezing parameters are optimized, and the nucleation effect of ice crystals in the freezing process of the collagen mixed solution is controlled by regulating and controlling parameters such as temperature and vacuum degree in the freeze-drying process, so that the shapes and the sizes of the crystals in the collagen freeze-dried product are more uniform, the drying time is shortened, and the production efficiency is improved. And the collagen freeze-dried product with uniform pore diameter and excellent appearance is obtained, and the uniformity and stability of batch and inter-batch products are improved.

The invention relates to a mulberry leaf flavone microcapsule as well as a preparation method and application thereof, and belongs to the technical field of microcapsule drug loading. The preparation method of the mulberry leaf flavone microcapsule comprises the following steps: S1, adding mulberry leaf flavone into a chitosan solution, carrying out ultrasonic treatment, shearing by using a homogenizer, and heating and stirring to obtain a mixed solution; s2, slowly dropwise adding a sodium tripolyphosphate solution into the mixed solution, reacting and curing until the solution becomes milk white liquid from clear and no precipitate is generated, then centrifuging and washing, and collecting particles; and S3, freezing the particles, and performing freeze drying to obtain the mulberry leaf flavone microcapsules. The mulberry leaf flavone microcapsule provided by the invention can be used for developing a novel medicinal preparation in the field of medicines, and can relieve disease symptoms and delay disease progression through the antioxidant and anti-inflammatory characteristics of the mulberry leaf flavone microcapsule; in the field of food, the method can be applied to development of functional food, for example, the method can be added into beverages and health-care products to provide health benefits of antioxidation, blood fat reduction and the like for consumers.

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The utility model relates to a reaction tube, in particular to a reaction tube for CRI SPR (CRI Surface Plasmon Resonance) detection. The reaction tube comprises a cover body and a tube body, the tube body comprises a tube body opening, the cover body can cover the tube body opening, the cover body comprises a containing cavity, and a solid reagent such as a Cas protein freeze-dried body or a CRI SPR detection system freeze-dried body is arranged in the containing cavity. The utility model solves the technical problem that the cover needs to be opened in two-step reaction, for example, the technical problem that the cover needs to be opened in a CRI SPR (CRI Surface Plasmon Resonance) detection two-step method.

The invention provides a decellularized dermal matrix particle and a preparation method thereof, the preparation method comprises the steps of pretreatment, degreasing, virus inactivation, decellularization, grinding, crosslinking, freeze-drying and sterilization subpackaging, and solves the problems that the existing decellularized particle product is non-uniform in particle size, incomplete in decellularization, poor in injection effect and incapable of meeting the requirements of tissue repair and regeneration. The invention develops a product which is short in production cycle, relatively concentrated in particle size distribution, thorough in cell removal and complete in matrix function preservation, can effectively remove fat and viruses in animal skin, and has the advantages of being thorough in decellularization, short in time, high in efficiency, light in damage to dermal matrix, capable of removing antigens, capable of retaining a natural structure and capable of effectively guiding tissue regeneration. When the temperature of the freeze-dried particle dispersion liquid is reduced, the water binding capacity of the particles is reduced, free water in the prepared solution is increased, the particles naturally float and gather (dense at the upper part and sparse at the lower part), and the free water is in the lower layer and still has good dispersity after being sterilized.

The invention relates to the technical field of biology, in particular to an anti-pollution freeze-dried eight-color Mini-STR composite amplification kit. Through design and optimization, the primer group, the reagent and the kit for detecting the eight-color multiple fluorescence labeling Mini-STR capable of identifying 25 STR gene loci are developed; the primer group, the reagent and the kit are used for detecting forensic samples, the detection sites are more, the detection accuracy is higher, the anti-pollution capacity is high, the detection capacity on trace samples is higher, the detection rate of degraded sample DNA can be remarkably increased, and the kit is higher in stability and has wide application value.

The invention discloses a winter jujube fruit fresh-keeping processing device, and belongs to the technical field of fruit fresh-keeping processing. The device is composed of a freeze-drying structure and a cold trap structure, the freeze-drying structure integrates quick freezing and freeze-drying functions, and the cold trap structure is connected with a vacuum system; during working, pretreated winter jujubes are placed on a specific storage plate frame and are quickly frozen in a quick-freezing bin of a freeze-drying structure, meanwhile, the internal special structure drives a liquid material to circulate to assist ice crystallization, ice crystals push the storage plate frame to enter the freeze-drying bin, automatic connection from quick freezing to freeze-drying is achieved, and after the winter jujubes enter the freeze-drying bin, related parts are matched to start vacuum and other systems, and the winter jujubes are quickly frozen. Performing freeze-drying and fresh-keeping processing on the winter jujubes; the device avoids loss of transfer cold energy, guarantees freeze-drying quality of winter jujubes, optimizes early-stage preparation of freeze-drying, improves fresh-keeping processing efficiency, and can also automatically clean ice crystals and adherends, prolong the service life of equipment and reduce maintenance cost.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention relates to a recombinant bacterium for overexpressing uranyl binding protein and application of the recombinant bacterium, and belongs to the technical field of microorganisms. Bacterial outer membrane protein and uranyl binding protein are expressed in host bacteria, and the uranyl binding protein is anchored on an outer membrane of recombinant bacteria through the bacterial outer membrane protein, so that the uranyl binding protein anchored on the bacterial outer membrane can adsorb uranyl ions. Meanwhile, the invention finds that the expression quantity of the uranyl binding protein can be increased by performing fusion expression on the uranyl binding protein by using LPP-OMPA, so that the adsorption effect of uranyl ions is improved. The recombinant bacterium is prepared into a freeze-dried capsule product, and the freeze-dried capsule product can penetrate through a stomach barrier and is accurately delivered to intestinal tracts so as to realize uranyl ion adsorption.

The invention discloses a preparation method and application of anti-photoaging collagen polypeptide. The preparation method comprises the following steps: unfreezing and cleaning silver carp skin, airing and cutting; naHCO3 is added, and soaking is carried out; adding Na2CO3 and carrying out ultrasonic impurity removal; performing ultrasonic-assisted treatment; adding protease for enzymolysis treatment; establishing an enzymolysis kinetic model under the alkaline protease condition; standing, cooling and centrifuging the enzymatic hydrolysate, collecting supernate, filtering, and performing vacuum freeze drying to obtain faint yellow SCSCP; the light aging resistant peptide SCSCP02 is separated, and the active peptide sequences of the light aging resistant peptide SCSCP02 are GPPGPPGTPGPQ, SGLPGPIGPPGPR and GLPGPIGPPGPR. The active substances which are extracted from the fish skin and have the anti-photoaging effect can reduce the risk of skin light damage caused by UVR by acting on various treatment targets, resource waste can be reduced, environmental pollution caused by biological resources can be relieved, and sustainable development of the industry can be promoted.

The invention relates to the technical field of freeze-drying control, and discloses a freeze-drying process control method and system based on data analysis, and the method comprises the steps: collecting temperature data, humidity data and pressure data in a freeze-drying box; preprocessing the temperature data, the humidity data and the pressure data to obtain preprocessed data; performing feature analysis on the preprocessed data through a principal component analysis method to obtain feature data; inputting the feature data into a pre-trained freeze-drying control model to obtain prediction parameters, the prediction parameters including prediction temperature, prediction humidity and prediction pressure; and controlling freeze-drying equipment according to prediction parameters, the temperature data, the humidity data and the pressure data. The method has the following effects that the intelligent degree of the freeze-drying process is improved, and then the freeze-drying efficiency is improved.

The invention belongs to the technical field of biological agents, and relates to an SOD freeze-dried preparation and a preparation method and application thereof. The invention provides an SOD (superoxide dismutase) freeze-drying preparation which is obtained by dissolving raw materials in a proper amount of solvent and then freeze-drying the raw materials, and the raw materials comprise SOD and collagen; the mass ratio of the SOD to the collagen is 1: (0.5-4). The collagen is added into the raw materials of the SOD freeze-drying preparation, so that the loss of the activity of SOD in the freeze-drying process can be well prevented, and meanwhile, the technical problem that the heat stability of the SOD freeze-drying preparation is insufficient is solved.

The invention belongs to the field of biology, and discloses a CRISPR / Cas12a freeze-dried microsphere, which is characterized in that the CRISPR / Cas12a freeze-dried microsphere is obtained by freeze-drying the following raw materials: enzyme-free water, 10 * HOLMES Buffer for Cas12a, an RNA enzyme inhibitor, CRISPR / Cas12a protein, crRNA, an upstream primer F, a downstream primer R, a fluorescent probe Reporter and a freeze-drying protective additive; the CRISPR / Cas12a protein is an LbaCas12a protein, and the CRISPR / Cas12a protein is a Cas12a protein; the freeze-drying protective agent is prepared from mannitol, trehalose, polyethylene glycol, glucan and BSA (Bovine Serum Albumin). By further optimizing a freeze-drying protective agent, freeze-drying microballing of a CRISPR / Cas12a system is achieved, the freeze-drying microballing can be matched with RPA constant-temperature amplification double microballing, and detection is conducted through a one-step method. Meanwhile, the invention further discloses a three-microsphere detection system, application and a kit.

The utility model relates to the technical field of food production, in particular to an integrated processing device for freeze-drying bean melting food, which comprises a top plate, guide posts, a bottom plate, a pouring head fixing plate, an upper template, a lower template, an ejector rod fixing plate, a pouring head fixing plate driving cylinder, an upper template driving cylinder and an ejector rod fixing plate driving cylinder, a pouring head fixing plate driving cylinder drives a pouring head fixing plate to do lifting motion, an upper mold plate driving cylinder drives an upper mold plate to do lifting motion, an ejector rod fixing plate driving cylinder drives an ejector rod fixing plate to do lifting motion, and materials formed through pouring are automatically demolded through interaction and cooperation. According to the freeze-drying bean melting machine, automatic production of freeze-drying bean melting food is achieved, compared with existing manual production, manufacturing time is shortened, production efficiency is improved, and large-batch production and sales are achieved.

The invention discloses a high-expansion cellulose sponge with an arch-like structure as well as a preparation method and application of the high-expansion cellulose sponge. The method comprises the following steps: taking carboxymethyl cellulose and cellulose or micro-nano fibers thereof as raw materials, and adding a cross-linking agent to prepare the hydrogel. The preparation method comprises the following steps: preparing a hydrogel, then carrying out bidirectional freezing-freeze-drying on the hydrogel to obtain a sponge sample, soaking the sponge sample in a solution capable of reacting with carboxyl, the solution comprising at least one of a calcium chloride solution, a chitosan solution or a mixed solution of the calcium chloride solution and the chitosan solution, and finally carrying out secondary freeze-drying to obtain the sponge. The sponge is good in biocompatibility and high in liquid absorption rate, and the volume expansion rate can reach 10-15 times; after being compressed, the bandage can be quickly expanded to block wounds and quickly stop bleeding when being applied to bleeding parts. An arch-like layer structure is arranged between layers of the sponge, so that high compression can be realized, and expansion supporting force as long as more than 4 hours can be provided. The sponge prepared by the invention can be used for deep bleeding, such as deep and narrow through wound bleeding, human body junction bleeding and the like.

It is provided a personal care product comprising two subproducts: a first subproduct which is a freeze-dried solid composition comprising a thickening agent and an humectant; and a second subproduct which is a dried solid film comprising a thickening agent and an humectant; wherein the second subproduct is adapted to contain the first product, thereby acting as a first enveloping element covering the first product, wherein the first product and the second product are adapted to be separately manipulated as two independent products, and each provide a different or equal personal care function by being water-soluble. The personal care product is suitable for cosmetic, oral care, dermatological and pharmaceutical use. Said product can further comprise a second enveloping element comprising a synthetic polymeric film and on the inner part a thickening agent. Furthermore, the present invention is directed to a method to prepare said product.

The invention discloses a vacuum freeze dryer, particularly relates to the technical field of vacuum freeze drying, and comprises a drying chamber, a refrigerating system, a vacuum system, a heating system, a control system and an energy recovery device. The drying chamber is used for containing materials, the inner wall of the drying chamber is made of efficient heat insulation materials, the refrigerating system comprises a high-precision temperature sensor and an intelligent temperature controller, the refrigerating temperature fluctuation is accurately controlled within + / -1 DEG C, the vacuum degree fluctuation of the vacuum system is kept within + / -5Pa through a vacuum degree sensor and a stabilizing device, and the heating system adopts uniform heating elements. And it is ensured that the heating temperature nonuniformity is smaller than + / -1 DEG C, a control system achieves automatic drying, and an energy recovery device recovers waste cold and waste heat for other links. The problems that an existing vacuum freeze dryer is low in temperature control precision, unstable in vacuum degree, low in automation degree, low in energy utilization rate and the like are solved, the material quality can be effectively kept, the drying efficiency is improved, and energy consumption is reduced.

The invention belongs to the technical field of Tibetan medicinal material processing, and particularly relates to a Tibetan medicinal material vacuum freeze-drying process which comprises the following steps: feeding a loading tray into a freeze-drying bin, and cooling at a speed of 1-2 DEG C / min; when the center temperature of the material reaches-35 DEG C, maintaining for 2-4 hours; a vacuum system is started, and the pressure in the bin is reduced to 10-30 Pa; the initial temperature of the heating plate is set to be-10 DEG C to 10 DEG C; real-time monitoring is carried out through an array type sensor network; vacuum breaking operation; through accurate perception, intelligent diagnosis, robust decision making and self-adaptive regulation and control, the quality problems of collapse, coking, active ingredient degradation and the like of the medicinal materials in the drying process can be effectively prevented, and high quality and high uniformity of a final product are ensured. Meanwhile, by optimizing a drying path, unnecessary energy consumption and time waste are avoided, the economic benefit of the whole production process is improved, and the method is particularly suitable for modern processing of precious Tibetan medicinal materials with extremely high quality requirements.

A refrigerating system of the novel freeze dryer comprises a low-pressure-stage compressor, a high-pressure-stage compressor, a condenser, an intercooler, a throttling valve, an electromagnetic valve, an auxiliary evaporator and a main evaporator. The waste heat collecting system comprises a main evaporator, a cold oil pump, a condenser, a hot oil pump, a regulating valve, an oil tank and a heat exchanger. The vacuumizing system comprises an oil pump, a drying box, a rotating wheel dehumidification box, a valve and a vacuum pump; the rotating wheel dehumidification system comprises a rotating wheel dehumidification box, a dehumidification rotating wheel, a vacuum pump, a fan and a regeneration heater; the drying efficiency is improved, the energy utilization rate of the system is remarkably improved, energy waste is reduced, the operation cost is reduced, it is ensured that the humidity environment in the freeze-drying process is more uniform and controllable, and the frosting phenomenon caused by low temperature in the dehumidification process is avoided.

The utility model relates to the technical field of royal jelly manufacturing, in particular to a freeze-drying device for manufacturing royal jelly freeze-dried powder buccal tablets, which comprises a freeze-drying box, a working cavity is arranged on the upper side in the freeze-drying box, a plurality of freeze-drying material boxes are arranged in the working cavity, feeding grooves are arranged in the freeze-drying material boxes, and the feeding grooves are communicated with the working cavity. Clamping blocks are slidably clamped to the outer walls of the two sides of the feeding groove, forming plates are arranged on the sides, close to each other, of the two clamping blocks, a plurality of forming holes are formed in each forming plate at equal intervals, a pressing plate is arranged at the top of each forming plate, and demolding pressing blocks are fixedly connected to the positions, corresponding to the forming holes, of the bottom face of each pressing plate. According to the royal jelly powder piece forming device, the freeze-drying material box, the clamping block, the feeding groove, the forming plate, the forming holes, the pressing plate and the demolding pressing block are arranged in a matched mode, royal jelly powder pieces solidified in the forming holes of the forming plate can be rapidly demolded, demolding is easy, and meanwhile the demolding time is shortened.

The application discloses a preparation method of a sandwich structure bio-based composite nanofiber aerogel material, and comprises the following steps: step 1), heating and stirring to dissolve low acyl gum; step 2), mixing the product obtained in step 1) with a hot aqueous solution of MXene; step 3), freeze-drying treatment of the aqueous solution of MXene to obtain a compressed layer of MXene; step 4), pasting and covering the product obtained in step 3) on an outer layer obtained in step 2), pouring the gum solution obtained in step 1) into the outer layer to obtain a sandwich structure composite hydrogel; step 5), soaking the product obtained in step 4) in a metal salt solution to harden; step 6), soaking the product obtained in step 5) in an organic solvent solution; and step 7), drying the product obtained in step 6) by using a supercritical liquid carbon dioxide drying method to obtain the sandwich structure bio-based composite nanofiber aerogel. The material has low thermal conductivity.

The invention provides an extraction method of hirudin, an oral preparation containing the hirudin and a preparation method of the oral preparation, and belongs to the technical field of leech processing and traditional Chinese medicine. The hirudin extraction method comprises the following steps: S1, soaking fresh leeches in a freeze-drying protective agent, taking out the leeches, wiping the surfaces of the leeches, pre-freezing the leeches at the temperature of 40 DEG C below zero to 50 DEG C below zero for 1-2 hours, and freeze-drying the leeches at the temperature of 20 DEG C below zero to 30 DEG C below zero for 4-6 hours to obtain freeze-dried leeches; s2, crushing the freeze-dried leeches, adding the crushed leeches into water, adding enzyme and lecithin, performing enzymolysis for 4-6 hours under the conditions that the temperature is 35-40 DEG C and the pH value is 7.5-8.5, and cooling to room temperature to obtain an enzymolysis product; s3, sterilization, ultrafiltration and drying are conducted, and hirudin is obtained. The hirudin extracted by the method is high in activity and high in recovery rate.

The invention discloses a freeze-drying method of long culture growth hormone, which comprises the following steps: adding a middle plug into a double-cavity clamping bottle, filling the double-cavity clamping bottle with the long culture growth hormone to be freeze-dried, and placing and fixing the double-cavity clamping bottle on a customized freeze-drying bracket; the top plug is placed in the plug pressing assembly so as to be fixed to the bottle opening of the double-cavity clamping type bottle; performing freezing, primary drying and secondary drying on the to-be-freeze-dried long culture growth hormone respectively; the top plug is fixed by the top cover and the bottle opening is sealed; the plug pressing assembly comprises a pressing-in jig and a maintaining jig, the maintaining jig is attached to the outer wall of the bottle opening, and the top plug is placed in the maintaining jig so that the top plug can be fixed to the bottle opening; the press-in jig presses the top plug to be embedded into the bottle opening, and the plug pressing assembly is removed to complete plug pressing. According to the method disclosed by the invention, high-quality freeze-drying parameters and freeze-drying operation can be provided, and the top plug and the top cover are utilized to complete plug pressing and cover sealing of the double-cavity clamping type bottle, so that the obtained growth hormone freeze-drying preparation for the growth culture keeps better appearance and lower moisture.

A lyophilization method for lyophilizing products inside one or more vials within a lyophilization chamber is disclosed which includes humidifying a charge gas to a predetermined relative humidity, cooling shelves in the lyophilization chamber to a predetermined temperature, pressurizing the chamber with the humidified charge gas to a pressurization threshold to thereby achieving a target relative humidity level within the lyophilization chamber, and suddenly releasing pressure within the lyophilization chamber until a depressurization threshold is reached in a short time interval up to about 4 seconds, during the depressurization, product inside one or more vials nucleate.

The invention provides a preparation method of a cordyceps militaris fermentation product with anti-aging, mitochondrial function enhancing, anti-oxidation and autophagy promoting effects, and the preparation method comprises the following steps: inoculating cordyceps militaris into a fermentation substrate for liquid fermentation culture to obtain a fermentation mycelium; the fermented mycelium is subjected to wall breaking or centrifugal separation, concentration and freeze-drying, and the cordyceps militaris fermented product is prepared; wherein the fermentation substrate comprises oat bran and / or lily. The cordyceps militaris fermentation product prepared by the method shows remarkable anti-aging, anti-oxidation, mitochondrial function enhancing and autophagy promoting effects in in-vitro cell experiments and in-vivo animal models, the process period is short, the preparation process is simple and easy, liquid fermentation in the whole process is suitable for large-scale production, and the preparation cost is low.