1968 results about "Freeze dry" patented technology

The invention provides a probiotics plant solid beverage for treating hyperuricemia and gout. The solid beverage is prepared by mixing freeze-dried probiotics powder and plant extract with the addition of proper auxiliary materials. The solid beverage comprises the following components: the plant extract, the auxiliary materials and the probiotics, with the sum of the mass percentage of each component being 100%. The plant extract comprises celery seed powder, sophora flower bud powder and platycodon root powder. The invention has an obvious effect on lowering uric acid and relieving gout.

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention belongs to the technical field of biological agents, and relates to an SOD freeze-dried preparation and a preparation method and application thereof. The invention provides an SOD (superoxide dismutase) freeze-drying preparation which is obtained by dissolving raw materials in a proper amount of solvent and then freeze-drying the raw materials, and the raw materials comprise SOD and collagen; the mass ratio of the SOD to the collagen is 1: (0.5-4). The collagen is added into the raw materials of the SOD freeze-drying preparation, so that the loss of the activity of SOD in the freeze-drying process can be well prevented, and meanwhile, the technical problem that the heat stability of the SOD freeze-drying preparation is insufficient is solved.

The invention belongs to the field of biology, and discloses a CRISPR / Cas12a freeze-dried microsphere, which is characterized in that the CRISPR / Cas12a freeze-dried microsphere is obtained by freeze-drying the following raw materials: enzyme-free water, 10 * HOLMES Buffer for Cas12a, an RNA enzyme inhibitor, CRISPR / Cas12a protein, crRNA, an upstream primer F, a downstream primer R, a fluorescent probe Reporter and a freeze-drying protective additive; the CRISPR / Cas12a protein is an LbaCas12a protein, and the CRISPR / Cas12a protein is a Cas12a protein; the freeze-drying protective agent is prepared from mannitol, trehalose, polyethylene glycol, glucan and BSA (Bovine Serum Albumin). By further optimizing a freeze-drying protective agent, freeze-drying microballing of a CRISPR / Cas12a system is achieved, the freeze-drying microballing can be matched with RPA constant-temperature amplification double microballing, and detection is conducted through a one-step method. Meanwhile, the invention further discloses a three-microsphere detection system, application and a kit.

The invention belongs to the technical field of Tibetan medicinal material processing, and particularly relates to a Tibetan medicinal material vacuum freeze-drying process which comprises the following steps: feeding a loading tray into a freeze-drying bin, and cooling at a speed of 1-2 DEG C / min; when the center temperature of the material reaches-35 DEG C, maintaining for 2-4 hours; a vacuum system is started, and the pressure in the bin is reduced to 10-30 Pa; the initial temperature of the heating plate is set to be-10 DEG C to 10 DEG C; real-time monitoring is carried out through an array type sensor network; vacuum breaking operation; through accurate perception, intelligent diagnosis, robust decision making and self-adaptive regulation and control, the quality problems of collapse, coking, active ingredient degradation and the like of the medicinal materials in the drying process can be effectively prevented, and high quality and high uniformity of a final product are ensured. Meanwhile, by optimizing a drying path, unnecessary energy consumption and time waste are avoided, the economic benefit of the whole production process is improved, and the method is particularly suitable for modern processing of precious Tibetan medicinal materials with extremely high quality requirements.

A refrigerating system of the novel freeze dryer comprises a low-pressure-stage compressor, a high-pressure-stage compressor, a condenser, an intercooler, a throttling valve, an electromagnetic valve, an auxiliary evaporator and a main evaporator. The waste heat collecting system comprises a main evaporator, a cold oil pump, a condenser, a hot oil pump, a regulating valve, an oil tank and a heat exchanger. The vacuumizing system comprises an oil pump, a drying box, a rotating wheel dehumidification box, a valve and a vacuum pump; the rotating wheel dehumidification system comprises a rotating wheel dehumidification box, a dehumidification rotating wheel, a vacuum pump, a fan and a regeneration heater; the drying efficiency is improved, the energy utilization rate of the system is remarkably improved, energy waste is reduced, the operation cost is reduced, it is ensured that the humidity environment in the freeze-drying process is more uniform and controllable, and the frosting phenomenon caused by low temperature in the dehumidification process is avoided.

The application discloses a preparation method of a sandwich structure bio-based composite nanofiber aerogel material, and comprises the following steps: step 1), heating and stirring to dissolve low acyl gum; step 2), mixing the product obtained in step 1) with a hot aqueous solution of MXene; step 3), freeze-drying treatment of the aqueous solution of MXene to obtain a compressed layer of MXene; step 4), pasting and covering the product obtained in step 3) on an outer layer obtained in step 2), pouring the gum solution obtained in step 1) into the outer layer to obtain a sandwich structure composite hydrogel; step 5), soaking the product obtained in step 4) in a metal salt solution to harden; step 6), soaking the product obtained in step 5) in an organic solvent solution; and step 7), drying the product obtained in step 6) by using a supercritical liquid carbon dioxide drying method to obtain the sandwich structure bio-based composite nanofiber aerogel. The material has low thermal conductivity.

The invention provides an extraction method of hirudin, an oral preparation containing the hirudin and a preparation method of the oral preparation, and belongs to the technical field of leech processing and traditional Chinese medicine. The hirudin extraction method comprises the following steps: S1, soaking fresh leeches in a freeze-drying protective agent, taking out the leeches, wiping the surfaces of the leeches, pre-freezing the leeches at the temperature of 40 DEG C below zero to 50 DEG C below zero for 1-2 hours, and freeze-drying the leeches at the temperature of 20 DEG C below zero to 30 DEG C below zero for 4-6 hours to obtain freeze-dried leeches; s2, crushing the freeze-dried leeches, adding the crushed leeches into water, adding enzyme and lecithin, performing enzymolysis for 4-6 hours under the conditions that the temperature is 35-40 DEG C and the pH value is 7.5-8.5, and cooling to room temperature to obtain an enzymolysis product; s3, sterilization, ultrafiltration and drying are conducted, and hirudin is obtained. The hirudin extracted by the method is high in activity and high in recovery rate.

The invention discloses a novel long-acting bird repellent and a preparation method thereof, and belongs to the technical field of chemical bird repelling. The bird repellent is composed of a core material and a wall material, the core material is a mixture of geraniol, cinnamyl aldehyde and allicin according to the mass ratio of 1: (1.2-1.5): (0.5-0.8), the wall material is modified beta-cyclodextrin and pumpkin seed protein nanoparticles, and the mass ratio of the geraniol, the cinnamyl aldehyde and the allicin is (5-7): (3-5): (1-3). The preparation method comprises the following steps: preparing the quaternary ammonium salt modified beta-cyclodextrin, including the core material, performing high-speed shearing emulsification on the quaternary ammonium salt modified beta-cyclodextrin and the pumpkin seed protein nano dispersion liquid, and performing freeze drying to obtain a final product. According to the invention, a stable and compact'molecular inclusion + physical barrier 'dual slow-release structure is constructed by utilizing the electrostatic interaction between the positively charged modified beta-cyclodextrin and the negatively charged protein nanoparticles, the release time of effective components is remarkably prolonged to 9 months or more, and the composite material has excellent rain wash resistance and ultraviolet resistance, and can be used for preparing a composite material with a good application prospect. And efficient, environment-friendly and long-acting bird repelling is realized.

The invention provides an enzymolysis preparation method of a milk-derived taste enhancing peptide, which comprises the following steps: carrying out enzymolysis on a liquid dairy product by using flavourzyme and glutaminase, and then carrying out enzyme deactivation to obtain an enzyme-deactivated product; carrying out acidification centrifugation and ultrafiltration on the enzyme-deactivated product, and freeze-drying the separated effluent to obtain freeze-dried powder; and loading the freeze-dried powder, and separating by a glucose gel column. The product disclosed by the invention does not generate bitter taste, and compared with a sample which is not subjected to enzymolysis, the flavor level of an original skimmed milk product is enriched, and the sample subjected to enzymolysis has the mellow taste of caramel and frankincense. The method comprises the following steps: separating milk-derived taste active peptides from milk-derived taste active peptides, predicting delicate flavor, anti-oxidation and anti-inflammatory activity and ACE blood pressure lowering functional activity of separated peptide liquid through a model, performing taste verification on the peptides predicted to have taste activity through Fmoc solid-phase synthesis, and performing sensory verification on the milk-derived taste active peptides LSFD, EDIKQME, IKQMEAE and TEDELQDK after synthesis, so that the milk-derived taste active peptides are obtained for the first time and are subjected to sensory verification.

The invention discloses a low-temperature vacuum microwave freeze-dried sinomenine hydrochloride preparation and a preparation method thereof in the technical field of pharmaceutical preparations, and solves the problems of long drying time, high energy consumption and poor product stability in the traditional freeze-drying technology. A vacuum microwave collaborative freeze-drying technology is adopted, microwave internal heating is achieved through a 2.45 GHz magnetron microwave generator and a rectangular waveguide tube under the conditions that the vacuum degree is 20-30 Pa and the plate layer temperature ranges from-40 DEG C to-35 DEG C, the microwave power density in the primary drying stage ranges from 0.3 W / g to 0.5 W / g, the microwave power density in the secondary drying stage ranges from 0.1 W / g to 0.3 W / g, and collaborative control over the microwave power, the vacuum degree, the temperature and the time is achieved in cooperation with a quaternary coupling precise control system. The preparation contains 50 mg of sinomenine hydrochloride, 100 mg of mannitol and 10 mg of sodium carboxymethyl cellulose grafted modified polyvinylpyrrolidone, the residual moisture is controlled to be 0.4-0.6%, the activity retention rate is 94-96%, and the drying time is shortened to 12-16 hours.

The invention relates to the technical field of freeze-drying control, and discloses an automatic control method and system for freeze-drying, rotating and pre-freezing. According to the method, a freeze-drying process dynamic model is constructed by monitoring operation parameters in a rotary pre-freezing stage, and characteristic indexes of the freeze-drying process dynamic model are analyzed; comparing the index with a standard reference value to generate a deviation analysis result, mining abnormal signals, screening key control points, monitoring environmental parameters in real time, and calculating response coefficients of the environmental parameters influencing the key control points; evaluating a potential fault mode based on the response coefficient, positioning a fragile link in the process, and carrying out performance detection to find out a specific problem point; formulating a control planning target according to the problem point, analyzing a corresponding control mode, querying a standard of the control mode, and generating a control operation instruction according to the standard; after the instruction is applied to equipment execution control, freeze-dried product quality data is collected for consistency verification, and finally a process quality report is generated. According to the method, multi-dimensional monitoring, fault early warning and accurate regulation and control of the freeze-drying, rotating and pre-freezing process are achieved.

The invention belongs to the technical field of preparation of biological materials, and particularly discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application of the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The preparation method comprises the following steps: dissolving freeze-dried sea cucumber flower powder in deionized water, adjusting the pH value to be alkaline, heating and stirring, centrifuging, discarding the precipitate, adjusting the pH value to be acidic, centrifuging, collecting the precipitate, and freeze-drying to obtain sea cucumber flower protein powder; preparing a reaction solution, adding simulated gastric juice and simulated intestinal juice, stirring for reaction, inactivating protease, centrifuging, taking supernatant, and freeze-drying to obtain sea cucumber flower self-assembled polypeptide; the preparation method comprises the following steps: preparing a solution from sea cucumber, adding FeCl2. 4H2O and ascorbic acid, adjusting the pH value to be acidic, carrying out heating reaction, centrifuging, discarding the supernatant, and freeze-drying to obtain the sea cucumber flower self-assembled polypeptide-iron nano-carrier. The invention discloses a sea cucumber flower self-assembled polypeptide-iron nano-carrier as well as a preparation method and application thereof. The carrier can promote absorption of an organism to iron, a relatively high iron ion release rate is maintained in an intestinal environment, and the in-vivo bioavailability of iron ions is improved.

The invention discloses a structural bionic space-time coupled axon guidance-synchronous degradation type nerve implant as well as a preparation method and application thereof. According to the preparation method, after collagen and polyvinyl alcohol are compounded, freeze-drying is performed through a specific freeze-drying process to obtain the composite nerve implant with a bunchy multi-channel and porous structure, so that the defect that the degradation rate of a current application product is too high is overcome. The degradation rate required by the composite nerve implant is regulated and controlled according to the proportion of the composite materials, the composite materials which are rapidly degraded can be designed at the two ends of the composite nerve implant with the long length, and the composite materials which are slowly degraded can be designed at the middle part of the composite nerve implant with the long length, so that the mechanical performance required by nerve regeneration is supported. The obtained nerve implant not only is beneficial to preventing cicatrix tissues from entering, but also can be used as a topographic clue of nerve regeneration related cells to guide nerve regeneration, in addition, the degradation rate of the material is synchronous with the nerve regeneration process, and finally, the material is continuously degraded along with nerve regeneration and is replaced by new tissues.

The invention relates to the technical field of collagen extraction, and provides a collagen extraction process for increasing hydroxyproline content, which sequentially comprises the steps of raw material pretreatment, deliming and degreasing, directional enzymolysis, gradient pressure-temperature synergistic extraction, purification, concentration and drying, a composite pretreatment agent containing a proline hydroxylase activator, EDTA-2Na and neutral protease is adopted in the raw material pretreatment; deashing and degreasing are combined with a chelating agent solution and ultrasonic treatment; a compound enzyme solution composed of trypsin, papain and proline hydroxylase is used for directional enzymolysis; a two-stage gradient pressure-temperature cooperation mode is adopted for extraction; purification is realized through combination of gel filtration chromatography and ion exchange chromatography; and finally carrying out freeze concentration and vacuum freeze drying to obtain the product. According to the process, through a multi-step synergistic effect, the content of hydroxyproline in collagen is remarkably increased, the product purity is high, natural functional characteristics are reserved, and the process is suitable for the fields of food, cosmetics, biological medicine and the like.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum with high yield of nano-selenium and application of the bifidobacterium longum in production of nano-selenium, the bifidobacterium longum with high yield of nano-selenium can convert inorganic selenium salt into nano-selenium, and the content of nano-selenium enriched in freeze-dried bacterial powder is up to 24000mg / kg. The selenium-rich feed additive can be directly used for selenium dietary supplements or selenium-rich animal product production, and has potential application prospects in the fields of selenium-rich food and feed additives.

The invention relates to the technical field of food sustained-release preparations, and particularly discloses a sour cherry powder sustained-release preparation with a stable uric acid reducing effect and a preparation process of the sour cherry powder sustained-release preparation. The sour cherry powder sustained-release preparation comprises a core active phase freeze-dried sour cherry powder, a sustained-release carrier phase, a synergistic function phase, an auxiliary regulation phase and a filler, the synergistic functional phase contains a celery seed extract, a curcumin-phospholipid complex, a prebiotic complex and the like, and all the components have a synergistic effect. The preparation method comprises the following steps: carrying out wall breaking, enzymolysis, purification and freeze-drying treatment on sour cherry fruits to obtain a core raw material, compounding the core raw material with other components, and carrying out slow-release carrier construction, coating granulation and other steps to prepare the microcapsule structure preparation. The preparation can be used for assisting in reducing the uric acid level, and has the advantages of high stability of active ingredients, continuous and stable uric acid reducing effect, consideration to intestinal health and the like; the preparation process adopts freeze-drying and micro-capsule coating technologies, effectively retains active ingredients, is controllable in operation, and is suitable for large-scale production.

According to the freeze-drying process of the dendrobium officinale solid beverage, psyllium seed husk powder and a trihydroxy fatty acid derivative are compounded to form a synergistic protection system, annealing and segmented freeze-drying processes are combined, ice crystal growth is inhibited on the molecular level, active ingredients are protected, a stable porous structure is constructed on the macroscopic level, and the dendrobium officinale solid beverage is prepared. Finally, the dendrobium officinale solid beverage which is high in active ingredient retention rate and good in color, luster and flavor is efficiently prepared, meanwhile, the freeze-drying period is remarkably shortened, and the production efficiency is improved.

The invention relates to the field of freeze-drying protective agents, and particularly discloses a vacuum freeze-drying protective agent for lactobacillus plantarum LS-41. The vacuum freeze drying protective agent for the lactobacillus plantarum LS-41 comprises the following components: 15 parts of dried skim milk, 3 parts of cane sugar, 1 part of PBS dry powder, 6-10 parts of trehalose, 0.2-0.4 part of ascorbic acid, 0.06-0.10 part of sodium glutamate, 8-12 parts of sorbitol and 100 parts of ultrapure water. Through the synergistic effect of the trehalose, the ascorbic acid, the sodium glutamate, the sorbitol, the skimmed milk powder and the cane sugar, and improvement and reasonable matching of the category and the concentration of the protective agents, the damage of the lactobacillus plantarum LS-41 in the vacuum freeze-drying process can be reduced, and the freeze-drying survival rate of the lactobacillus plantarum LS-41 is increased.

The invention discloses bovine lactoferrin freeze-dried powder and a preparation method thereof, and belongs to the technical field of protein freeze-drying. The preparation method of the bovine lactoferrin freeze-dried powder comprises the following steps: adding a histidine buffer solution into a bovine lactoferrin solution with the mass concentration of 30-40%, and mixing to obtain a mixed solution; adding a freeze-drying auxiliary material accounting for 0.25-7.0% of the volume of the mixed solution into the mixed solution to obtain a bovine lactoferrin freeze-drying solution; performing spray freezing and freeze drying on the bovine lactoferrin freeze-dried liquid to obtain bovine lactoferrin freeze-dried powder; the freeze-drying auxiliary materials comprise a surfactant, amino acid, D-chiro-inositol and calcium chloride. Compared with the traditional inositol, the D-chiral inositol in the freeze-dried auxiliary material can more effectively replace water molecules, maintain the natural conformation of protein and prevent the bovine lactoferrin structure from collapsing. The calcium chloride in the freeze-drying auxiliary material can stabilize the iron binding area of the bovine lactoferrin in the freeze-drying process, and the biological activity of the bovine lactoferrin after freeze-drying is guaranteed.

The invention discloses a rapid amplification reagent microsphere and a kit, and belongs to the field of gene detection, and the rapid amplification reagent microsphere contains amplification primer sequences SEQ ID NO: 01 and SEQ ID NO: 02. The invention has the advantages of ready-to-use, freeze-dried spherical shape, easy preservation (room temperature preservation), simple operation (redissolving with water) and low cost, and can avoid the problem of inaccurate sequencing quality of about 100bp before and after a first-generation sequencing reaction when being used for sequencing.

The invention relates to the technical field of detection kits, in particular to an HbA1c detection kit and a preparation method thereof, and the kit comprises latex microsphere freeze-dried powder DP-1, HbA1c monoclonal antibody freeze-dried powder DP-2, buffer solution dry powder H-1 and buffer solution dry powder H-2. A composite preparation scheme of combining a microsphere and antibody freeze-drying process and a buffer reagent drying process is adopted, on one hand, the traditional biochemical reagent freeze-drying process is greatly simplified through process optimization, and the production time consumption and the cost investment of the freeze-drying link are effectively reduced; and on the other hand, the cold chain dependence in the product storage and transportation process is remarkably reduced due to the dry reagent form, and the overall transportation cost and loss risk are greatly reduced.

The invention relates to the technical field of preparation of food additives, in particular to a betacyanin microcapsule as well as a preparation method and application thereof. The preparation method comprises the following steps: jointly dissolving sodium alginate and soybean protein isolate in water to obtain an SA-SPI compound; the SA-SPI compound, CaCO3 and betacyanin are mixed, and a water phase is formed; mixing liquid paraffin, glacial acetic acid and Tween 80 to obtain an acetic acid-containing oil phase; mixing the water phase with the acetic acid-containing oil phase to form a water-in-oil emulsion, and standing to form calcium alginate gel beads; the calcium alginate gel beads are subjected to pre-freezing-freeze-drying treatment, and the betacyanin microcapsules are obtained. The prepared betacyanin microcapsule has the characteristics of controllable particle size, high stability and easiness in industrialization, and the application range of betacyanin is widened. In addition, the preparation method disclosed by the invention is safe and simple to operate, has the advantages of low cost and high benefit, and can be directly applied to large-scale production.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention relates to a composition for promoting collagen generation based on sophora flower powder as well as a preparation method and application of the composition, and belongs to the technical field of sophora flower extracts. A preparation method of the sophora flower powder extract comprises the following steps: taking sophora flower powder, adding water, boiling, cooling, adding a carbon source and aspergillus oryzae CICC 2066, fermenting, sterilizing, filtering, concentrating and freeze-drying to obtain the sophora flower powder extract. The sophora flower powder extract can be applied to food, can also be applied to cosmetics, and can effectively promote the generation of collagen and improve the skin elasticity and tightening effect.

The invention discloses stem cell freeze-dried powder for promoting skin healing and a preparation method of the stem cell freeze-dried powder. The freeze-dried powder comprises an effective component, a freeze-drying protective agent group and a polymer protective agent group, the effective component is umbilical cord mesenchymal stem cell conditioned medium freeze-dried supernatant, the protective agent group is selected from one or more of cane sugar, trehalose, lactose and mannitol, and the polymer protective agent group is selected from one or more of polyvinylpyrrolidone (PVP) and glucan. The preparation method comprises the following steps: collection of a conditioned culture medium, preparation of a freeze-drying protective agent solution, mixing treatment, low-temperature pre-freezing and freeze-drying, collection and packaging of freeze-dried powder and the like. The method can effectively retain stem cell-derived active factors and enhance the stability and redissolvability of the freeze-dried powder, and is suitable for skin wound repair, regenerative medicine and other scenes.

The invention discloses a freeze-drying protective agent composition, a freeze-drying protective agent, application of the freeze-drying protective agent, a probiotic freeze-dried product and a preparation method of the probiotic freeze-dried product. The freeze-drying protective agent composition comprises the following components in parts by weight: 0.5-4 parts of tremella polysaccharide; the relative molecular weight of the tremella polysaccharide is 1 KDa to 10 KDa; 0.5 to 4 parts of disaccharide; and 0.5-2 parts of sugar alcohol. The freeze-drying protective agent composition and water are uniformly mixed and then sterilized to obtain the freeze-drying protective agent, and the freeze-drying protective agent is used for preparing probiotic freeze-dried products and can effectively ensure the activity and stability of probiotics.

The invention relates to the technical field of functional food, and discloses a cranberry polyphenol and vitamin C corn germ compound beverage and a preparation method thereof, the cranberry polyphenol and vitamin C corn germ compound beverage is prepared from corn germ concentrated powder, corn oligopeptide powder, cranberry fermentation extract, L-ascorbic acid and gamma-aminobutyric acid as main raw materials, and cranberry pulp is subjected to lactobacillus fermentation and concentration; performing low-temperature spray drying on L-ascorbic acid and part of the corn oligopeptide powder solution to prepare vitamin C microcapsule powder; premixing the micro-capsule powder with other powder raw materials, and carrying out wet granulation by using a cranberry fermentation concentrated solution as a bonding medium; and finally, carrying out vacuum freeze drying on the wet granules. Physical isolation of the L-ascorbic acid is formed through microencapsulation treatment, and the chemical stability of the L-ascorbic acid is improved; the adopted low-temperature process combination maintains the biological activity of zymoprotein in the corn germs; the wet granulation process ensures the uniform distribution of each component, and endows the finished product with excellent physical properties and reconstituability.

The invention discloses an emulsifier for preparing a Pickering emulsion, the Pickering emulsion as well as a preparation method and application of the Pickering emulsion. The emulsifier of the Pickering emulsion is obtained by dissolving distearoyl phosphatidylcholine, cholesterol and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 in ethanol, carrying out rotary evaporation to remove ethanol, then adding an antigen aqueous solution containing an antigen, continuing rotary evaporation to obtain liposome nanoparticles, heating the liposome nanoparticles with mannose in a water bath, and carrying out freeze drying. The Pickering emulsion is obtained by dispersing an emulsifier in water as a water phase, taking squalene as an oil phase, mixing the water phase and the oil phase, and homogenizing. The Pickering emulsion disclosed by the invention is prepared by taking antigen presenting cell targeted liposome nanoparticles as a raw material; and the antigen has excellent ion concentration characteristic, pH stability, temperature stability and storage stability, and can protect the integrity of the antigen.