1123 results about "Freeze dry" patented technology

The invention provides a probiotics plant solid beverage for treating hyperuricemia and gout. The solid beverage is prepared by mixing freeze-dried probiotics powder and plant extract with the addition of proper auxiliary materials. The solid beverage comprises the following components: the plant extract, the auxiliary materials and the probiotics, with the sum of the mass percentage of each component being 100%. The plant extract comprises celery seed powder, sophora flower bud powder and platycodon root powder. The invention has an obvious effect on lowering uric acid and relieving gout.

The invention belongs to the field of biology, and discloses a CRISPR / Cas12a freeze-dried microsphere, which is characterized in that the CRISPR / Cas12a freeze-dried microsphere is obtained by freeze-drying the following raw materials: enzyme-free water, 10 * HOLMES Buffer for Cas12a, an RNA enzyme inhibitor, CRISPR / Cas12a protein, crRNA, an upstream primer F, a downstream primer R, a fluorescent probe Reporter and a freeze-drying protective additive; the CRISPR / Cas12a protein is an LbaCas12a protein, and the CRISPR / Cas12a protein is a Cas12a protein; the freeze-drying protective agent is prepared from mannitol, trehalose, polyethylene glycol, glucan and BSA (Bovine Serum Albumin). By further optimizing a freeze-drying protective agent, freeze-drying microballing of a CRISPR / Cas12a system is achieved, the freeze-drying microballing can be matched with RPA constant-temperature amplification double microballing, and detection is conducted through a one-step method. Meanwhile, the invention further discloses a three-microsphere detection system, application and a kit.

The invention belongs to the technical field of Tibetan medicinal material processing, and particularly relates to a Tibetan medicinal material vacuum freeze-drying process which comprises the following steps: feeding a loading tray into a freeze-drying bin, and cooling at a speed of 1-2 DEG C / min; when the center temperature of the material reaches-35 DEG C, maintaining for 2-4 hours; a vacuum system is started, and the pressure in the bin is reduced to 10-30 Pa; the initial temperature of the heating plate is set to be-10 DEG C to 10 DEG C; real-time monitoring is carried out through an array type sensor network; vacuum breaking operation; through accurate perception, intelligent diagnosis, robust decision making and self-adaptive regulation and control, the quality problems of collapse, coking, active ingredient degradation and the like of the medicinal materials in the drying process can be effectively prevented, and high quality and high uniformity of a final product are ensured. Meanwhile, by optimizing a drying path, unnecessary energy consumption and time waste are avoided, the economic benefit of the whole production process is improved, and the method is particularly suitable for modern processing of precious Tibetan medicinal materials with extremely high quality requirements.

The invention discloses a low-temperature vacuum microwave freeze-dried sinomenine hydrochloride preparation and a preparation method thereof in the technical field of pharmaceutical preparations, and solves the problems of long drying time, high energy consumption and poor product stability in the traditional freeze-drying technology. A vacuum microwave collaborative freeze-drying technology is adopted, microwave internal heating is achieved through a 2.45 GHz magnetron microwave generator and a rectangular waveguide tube under the conditions that the vacuum degree is 20-30 Pa and the plate layer temperature ranges from-40 DEG C to-35 DEG C, the microwave power density in the primary drying stage ranges from 0.3 W / g to 0.5 W / g, the microwave power density in the secondary drying stage ranges from 0.1 W / g to 0.3 W / g, and collaborative control over the microwave power, the vacuum degree, the temperature and the time is achieved in cooperation with a quaternary coupling precise control system. The preparation contains 50 mg of sinomenine hydrochloride, 100 mg of mannitol and 10 mg of sodium carboxymethyl cellulose grafted modified polyvinylpyrrolidone, the residual moisture is controlled to be 0.4-0.6%, the activity retention rate is 94-96%, and the drying time is shortened to 12-16 hours.

The invention relates to the technical field of freeze-drying control, and discloses an automatic control method and system for freeze-drying, rotating and pre-freezing. According to the method, a freeze-drying process dynamic model is constructed by monitoring operation parameters in a rotary pre-freezing stage, and characteristic indexes of the freeze-drying process dynamic model are analyzed; comparing the index with a standard reference value to generate a deviation analysis result, mining abnormal signals, screening key control points, monitoring environmental parameters in real time, and calculating response coefficients of the environmental parameters influencing the key control points; evaluating a potential fault mode based on the response coefficient, positioning a fragile link in the process, and carrying out performance detection to find out a specific problem point; formulating a control planning target according to the problem point, analyzing a corresponding control mode, querying a standard of the control mode, and generating a control operation instruction according to the standard; after the instruction is applied to equipment execution control, freeze-dried product quality data is collected for consistency verification, and finally a process quality report is generated. According to the method, multi-dimensional monitoring, fault early warning and accurate regulation and control of the freeze-drying, rotating and pre-freezing process are achieved.

The invention relates to the technical field of collagen extraction, and provides a collagen extraction process for increasing hydroxyproline content, which sequentially comprises the steps of raw material pretreatment, deliming and degreasing, directional enzymolysis, gradient pressure-temperature synergistic extraction, purification, concentration and drying, a composite pretreatment agent containing a proline hydroxylase activator, EDTA-2Na and neutral protease is adopted in the raw material pretreatment; deashing and degreasing are combined with a chelating agent solution and ultrasonic treatment; a compound enzyme solution composed of trypsin, papain and proline hydroxylase is used for directional enzymolysis; a two-stage gradient pressure-temperature cooperation mode is adopted for extraction; purification is realized through combination of gel filtration chromatography and ion exchange chromatography; and finally carrying out freeze concentration and vacuum freeze drying to obtain the product. According to the process, through a multi-step synergistic effect, the content of hydroxyproline in collagen is remarkably increased, the product purity is high, natural functional characteristics are reserved, and the process is suitable for the fields of food, cosmetics, biological medicine and the like.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum with high yield of nano-selenium and application of the bifidobacterium longum in production of nano-selenium, the bifidobacterium longum with high yield of nano-selenium can convert inorganic selenium salt into nano-selenium, and the content of nano-selenium enriched in freeze-dried bacterial powder is up to 24000mg / kg. The selenium-rich feed additive can be directly used for selenium dietary supplements or selenium-rich animal product production, and has potential application prospects in the fields of selenium-rich food and feed additives.

According to the freeze-drying process of the dendrobium officinale solid beverage, psyllium seed husk powder and a trihydroxy fatty acid derivative are compounded to form a synergistic protection system, annealing and segmented freeze-drying processes are combined, ice crystal growth is inhibited on the molecular level, active ingredients are protected, a stable porous structure is constructed on the macroscopic level, and the dendrobium officinale solid beverage is prepared. Finally, the dendrobium officinale solid beverage which is high in active ingredient retention rate and good in color, luster and flavor is efficiently prepared, meanwhile, the freeze-drying period is remarkably shortened, and the production efficiency is improved.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention relates to the technical field of intelligent control, in particular to an intelligent control system for a freeze-drying process based on an intelligent algorithm, which comprises an acquisition module, a freezing adjustment module, a primary adjustment module and a secondary adjustment module. According to the method, the corrected plate layer temperature after incomplete freezing is accurately recognized according to double criteria of the freezing center temperature and the temperature drop trend, and after the drying efficiency deviation degree is calculated and the abnormal type is determined by utilizing the preset efficiency interval, the related preset process conditions are adjusted; meanwhile, stagnation and overheating runaway are independently diagnosed and analyzed on the basis of time sequence correlation of the water content decreasing rate and the center temperature increasing rate, the preorder freezing defect is traced when secondary drying is normal but overtime does not reach the standard, and the slab layer temperature is reduced in an equal proportion according to the freezing defect degree; the problem that the process efficiency and the product quality stability are difficult to consider at the same time due to dependence on artificial experience, stage splitting and lack of dynamic feedback is effectively solved.

The invention discloses a freeze-dried essence with a repairing effect and a preparation method, and belongs to the field of skin care products, and the freeze-dried essence is composed of freeze-dried powder and an independent solvent liquid. The freeze-dried powder contains 0.5-3 parts of palmitoyl oligopeptide, 3-8 parts of I-type collagen, 1-5 parts of III-type collagen, 0.1-1 part of chitosan, 1-3 parts of secondary variable-temperature fermentation liquor of betula platyphylla and 2-8 parts of a freeze-drying protective agent; the solvent liquid is prepared from 0.5 to 3 parts of hyaluronic acid and 0.05 to 0.5 part of sodium polyglutamate. The preparation method comprises the following steps: a, adding fermentation liquor after dissolving; b, adding nano liposome and a protective agent; c, quickly freezing to-55 + / -2 DEG C by liquid nitrogen, and preserving heat for 2.5-3.5 hours; d, drying for 9-11 hours under the vacuum of 15-25 Pa and at the temperature of-18 + / -2 DEG C; and e, the temperature is increased to 15 + / -2 DEG C in a stepped mode, vacuum is smaller than or equal to 20 Pa, and drying is conducted for 18- Through scientific formula design, multiple effects of efficient repairing, anti-aging and relieving are achieved.

The invention belongs to the technical field of fruits, vegetables and food, and discloses a method for recovering grape drying wastewater based on low-temperature freeze-drying and application, the method comprises the steps of selecting grapes, taking the grapes, cleaning, wiping and pre-freezing, drying the grapes by freeze-drying, and recovering water generated in the process to obtain grape freeze-drying recovered water. According to the method for recovering the moisture through low-temperature freeze drying, the lost moisture in the raisin processing process can be efficiently recovered, and the obtained condensate water keeps natural fragrance and nutritional activity, can be directly used for food and daily chemical industries and has remarkable economic and ecological benefits. According to the method, the comprehensive utilization rate of the fruit and vegetable drying process is increased, the application field of fruit and vegetable processing byproducts is expanded, a new solution is provided for green and sustainable development of the food industry, and the problems of waste water recycling and reutilization in the fruit and vegetable drying process are solved.

The invention discloses a preparation method of fermented quinoa post-generation powder, and belongs to the technical field of functional food raw materials. The method comprises the following steps: mixing a quinoa base material (quinoa bran powder and / or quinoa flour) with water to prepare slurry with the solid content of 10-40% (w / w); carrying out heating and gelatinization treatment on the slurry at 75-100 DEG C for 5-60 minutes, and then cooling to 30-45 DEG C; lactic acid bacteria are inoculated for fermentation, the fermentation temperature is 25-45 DEG C, the fermentation time is 4-48 h, and the pH value at the fermentation end point is controlled to be 3.6-4.6; after the fermentation is finished, carrying out inactivation treatment on a fermentation product at 70-100 DEG C for 1-60 minutes; and drying in a spray drying or freeze-drying manner to obtain the fermented quinoa post-raw powder, wherein the water content of the obtained powder is less than or equal to 10%. According to the method disclosed by the invention, the cereal raw taste and the coarse flour feeling of the quinoa powder can be remarkably improved, the brewing dispersity is improved, the sedimentation is reduced, and the post-prebiotics base powder raw material suitable for normal-temperature storage and development of downstream solid beverages, nutrition powder and other products is obtained.

Disclosed in the present invention are a preparation method for a shear thickening fluid and a use of the fluid. The preparation of the fluid comprises: first dispersing nanoparticles in a micromolecular alcohol pretreatment solvent, then centrifuging and freeze-drying to obtain freeze-dried powder, slowly adding the nanoparticle freeze-dried powder into polyhydric alcohol, slowly stirring and mixing until no macroscopic lump exists, then uniformly mixing by using a vortex oscillator or a bottle rolling machine to prepare a fluid with good uniformity, and finally drying and removing impurities to make the total content of water and micromolecular alcohol impurities not exceed 4 wt %, thereby obtaining a shear thickening fluid.

According to embodiments, a modified freeze-drying apparatus and methods of use are provided herein for providing variable power heating for precise control of material temperatures during freeze-drying. The apparatus may provide a vacuum pump and variable current heater trays for receiving the material, freezing the material, creating a vacuum, progressively increasing the heater tray temperature without causing temperature cycling, deactivating the tray heater, repressurizing the apparatus, and removing the freeze-dried material.

The invention belongs to the field of preparation of medicaments for reducing uric acid, and particularly relates to a traditional Chinese medicine composition for reducing uric acid level and a preparation method thereof. Piperine, oat beta-glucan and medium chain triglyceride are mixed to react, a carrier material with a net structure is formed, a uric acid reducing solution obtained through adsorption and fermentation is freeze-dried, and the obtained efficient uric acid reducing component can remarkably and efficiently reduce the uric acid level in an organism; the regulating component is mixed with the metabolism promoting component obtained after powdering, the mixture is put into the coating material which can form a transparent film after being dried, and freeze spray drying is performed to obtain the long-acting agent which can stably play a role in a long-acting manner; the composition for maintaining stability and improving taste is uniformly sprayed on the surface of the traditional Chinese medicine component for reducing the uric acid level, and the prepared traditional Chinese medicine composition for reducing the uric acid level is remarkable and efficient in effect, improved in taste, free of peculiar smell, beneficial to persistent taking and free of irritation to liver and kidney after being taken for a long time.

The invention discloses a self-emulsifying adjuvant of a vaccine and a preparation method of the self-emulsifying adjuvant, and relates to the technical field of vaccine adjuvants. The adjuvant comprises an oil phase, an emulsifier and a freeze-drying protective agent, the oil phase comprises squalene and tocopherol, the emulsifier comprises polysorbate and a polyoxyethylene-polyoxypropylene block copolymer, and the freeze-drying protective agent comprises sugar and sugar alcohol. The preparation method comprises the following steps: emulsifying an oil phase and a water phase to form a nano-emulsion, and then freeze-drying to obtain freeze-dried powder. The adjuvant has the advantages of small particle size, uniform distribution, high stability, rapid reconstruction and the like, and can effectively maintain active components and improve the immune effect of vaccines.

The invention belongs to the technical field of preparation of high-temperature heat-insulating and electromagnetic wave-absorbing materials, and relates to a bamboo-like silicon carbide nanowire based on freeze-dried vegetables / fruits and a preparation method and application of the bamboo-like silicon carbide nanowire based on the freeze-dried vegetables / fruits, and the preparation method comprises the following steps: putting the vegetables / fruits in a freeze dryer to obtain the freeze-dried vegetables / fruits; putting the freeze-dried vegetables / fruits into a high-temperature tubular furnace for carbonization to obtain a carbon template; putting the carbon template and a silicon source into a graphite crucible, putting the graphite crucible into a high-temperature tubular furnace, and calcining to obtain the bamboo-like silicon carbide nanowire based on the freeze-dried vegetables / fruits. The bamboo-like silicon carbide nanowire prepared by the method has excellent high-temperature heat insulation property and remarkable broadband electromagnetic wave absorption property, so that the enhancement of double functions of heat insulation and wave absorption is synergistically realized on the basis of a single material. The preparation process is simple and stable in flow, good in repeatability, green in raw material and extremely low in cost, and the problems of collapse and atrophy of the material structure caused by high temperature are solved.

The invention discloses a freeze-dried donkey milk powder quality control method and system based on digital twinning, and relates to the technical field of data twinning. Temperature, pressure, moisture content characterization, surface spectrum and visual information of donkey milk in the freeze-drying process are obtained through a multi-mode sensor, and are input into a digital twinning engine after edge preprocessing; and a virtual freeze-drying process is constructed in real time in combination with a heat and mass transfer model and a physical property database. A data assimilation algorithm is used for carrying out inversion estimation on key states which cannot be directly measured, such as the deep water content, the ice crystal interface position and the filming risk, and the heating rate, the vacuum level and the secondary drying time are dynamically optimized based on a predictive control strategy, so that closed-loop control of the whole process is realized. The system is further provided with a confidence evaluation module, an abnormal rollback module and a post-batch self-learning module, and online correction is performed on model deviation and continuous optimization is performed on a control strategy. According to the invention, the consistency between batches of products can be improved, and intelligent and high-quality production of the freeze-dried donkey milk powder is realized.

The invention discloses preparation and application of a rabies virus G protein mRNA vaccine freeze-drying preparation, and relates to the field of veterinary biological products. According to the invention, rabies virus G protein mRNA is prepared on a large scale through an in-vitro transcription technology, and an mRNA-LNP (lipid nanoparticle) delivery system is successfully constructed by adopting a microfluidic encapsulation process. Through freeze-drying protective agent formula screening (including cane sugar, trehalose and mannitol) and freeze-drying procedure parameter optimization (including pre-freezing temperature, main drying rate and secondary drying residual moisture control), the freeze-dried mRNA vaccine preparation which can stably exist under the condition of 4 DEG C is finally obtained.

The invention provides a bovine colostrum probiotic compound preparation as well as a preparation method and application thereof. The compound preparation provided by the invention comprises the bovine colostrum powder subjected to activity protection and the compound probiotics, and the bovine colostrum powder subjected to activity protection is obtained by adopting a microcapsule double-layer embedding and freeze-drying technology, so that the gastric acid retention rate (2h) of immune globulin in the bovine colostrum powder is greater than or equal to 90%, and IgG contained in the bovine colostrum powder is ensured to be efficiently delivered to intestinal tracts to play an immune regulation role. Wherein the compound probiotics comprise four stages of functional echeles formed by more than or equal to 6 strains, and the four stages of functional echeles comprise colon colonization major flora, gastric acid-resistant pioneer flora, antibacterial peptide secretion auxiliary flora and inflammation regulation backup flora. Through cooperation of the components in the compound preparation, the compound preparation has excellent immune enhancement and metabolic regulation functions.

The present application provides a probiotic microcapsule and a preparation method thereof. The probiotic microcapsule comprises a core material made of probiotics, a freeze-drying protective layer made of a freeze-drying protective agent and a wall material made of polysaccharides, wherein the freeze-drying protective layer is interposed between the core material and the wall material. The freeze-drying protective agent used in the probiotic microcapsule of the present application can reduce the damage to the bacterial body in the freezing and drying process through synergistic effect; and the wall material can form an acid-stable, dense and hard shell outside the bacterial body, greatly improving the stress resistance and stability of the probiotic in the production, storage and digestion processes. The probiotic microcapsule of the present application can be used for preparing food, cosmetics, medicines and feed.

The invention discloses a combined drying method for maintaining color and nutrition of mango freeze-dried products, and belongs to the technical field of food processing. The preparation method comprises the following steps: cutting mangoes, performing vacuum impregnation in a composite protection solution containing maltodextrin, trehalose, L-ascorbic acid and citric acid, then performing quick-freezing treatment, and finally adopting a combined drying process combining microwave vacuum drying and vacuum freeze drying to reduce the water content of the materials to the final requirement in two stages. The method is mainly used for efficiently producing a high-quality mango freeze-dried product which can perfectly maintain the natural color, shape and nutritional ingredients of mangoes; the technical problems that the drying period is too long, the energy consumption is high, and color browning and heat-sensitive nutritional ingredient degradation are prone to occurring in the long drying process when mangoes are treated through a traditional freeze-drying technology are effectively solved.

The invention provides a ZIF-67 (at) Co / HPLFM composite material and a preparation method and application of the ZIF-67 (at) Co / HPLFM composite material, and the preparation method comprises the following steps: providing a hollow porous straw lignin fiber membrane (HPLFM); cobalt salt is dissolved in absolute ethyl alcohol, the hollow porous straw lignin fiber membrane is taken and placed in the mixed solution, absolute ethyl alcohol dissolved with 2-methylimidazole is dropwise added into the mixed solution after oscillation, and oscillation continues for a period of time; taking out the hollow porous straw lignin fiber membrane, washing, and freeze-drying to obtain a precursor material; and carrying out high-temperature calcination on the precursor material to obtain the ZIF-67 (at) Co / HPLFM composite material. The high surface activity and the porous hollow structure of the hollow porous straw lignin fiber membrane are utilized, ZIF-67 is loaded on HPLFM, the ZIF-67-coated Co / HPLFM composite material is obtained through further treatment, and the material has high specific surface area and excellent adsorption performance and has good application prospects in organic pollutant degradation by activating peroxymonosulfate.

The invention discloses a compound microorganism freeze-drying protective agent, a preparation method of the compound microorganism freeze-drying protective agent and a use method of the compound microorganism freeze-drying protective agent. Comprising the following raw materials in parts by weight: 0.03 part of disodium ethylene diamine tetraacetate, 5 to 15 parts of trehalose, 2 to 5 parts of sorbitol, 0.5 to 2 parts of ascorbic acid, 0.05 to 0.2 part of N-acetyl-L-cysteine, 0.1 to 0.5 part of polylactic acid-glycolic acid copolymer, 0.01 to 0.05 part of alpha-tocopherol and 1 to 3 parts of bentonite. The PLGA, the alpha-tocopherol and the NAC construct a three-stage synergistic anti-oxidation system from outside to inside: the PLGA is externally blocked, so that rapid consumption of an antioxidant is avoided, and slow release is realized; the alpha-tocopherol realizes oxidative damage repair of cell membranes; nAC maintains the internal reduction environment of cells, reduces oxidative stress and can regenerate alpha-tocopherol at the same time, and reutilization of resources is achieved.

This invention discloses a composite method for improving the floating property of egg drop soup in freeze-dried egg drop soup, belonging to the field of food processing technology. This method solves the problems of egg drop settling and uneven dispersion after brewing in existing freeze-dried egg drop soups through the synergistic effect of egg liquid pretreatment, composite colloid addition, egg drop-soup pre-cooking, and integrated freeze-drying process optimization (including partition heating temperature control). Simultaneously, it achieves integrated freeze-drying of the egg drop and soup base. Freeze-dried egg drop soup treated by this method, when brewed in hot water at 85-95℃, exhibits an egg drop floating rate of over 90%, with high integration of the egg drop and soup base, maintaining good taste and appearance, significantly improving the product's eating experience and market competitiveness.

The invention belongs to the field of pharmaceutical preparations. The invention relates to a novel dosage form of an isavuconazole sulfate freeze-dried tablet, and a prescription and a process for preparing the isavuconazole sulfate freeze-dried tablet by adopting a freeze-drying method. The isavuconazole sulfate freeze-dried tablet is prepared from isavuconazole sulfate serving as a main drug and pharmaceutic adjuvants. Water is not needed during taking, and the tablet can be rapidly disintegrated after being put in the mouth and is suitable for patients with dysphagia such as old people and children; the production process of the isavuconazole sulfate freeze-dried orally disintegrating tablet is simple in technology, easy to control and suitable for industrial production.

The present invention provides a composition comprising collagenase as an enzyme; calcium, histidine, and glycine as coenzymes, and a method for stabilizing enzymes, the method comprising the preparation of the composition by adding histidine and glycine to collagenase. According to the present invention, the enzyme composition and method for stabilizing enzymes can minimize or inhibit the agglutination of enzymes, maintain the concentration of freeze-dried enzymes even during long-term storage, and provide a formulation that has excellent rehydration time and hygroscopicity when restored to a liquid state, as well as superior enzyme activity.

The invention provides an extraction method of coconut globulin, and relates to the technical field of coconut protein extraction. The method for extracting the coconut globulin comprises the following steps: removing albumin from defatted coconut powder by using distilled water, performing nitrogen pressure-boosting drying, mixing with a NaCl solution with relatively low concentration, performing high-voltage pulse electric field circulation treatment, stirring and extracting the NaCl solution, freeze-drying, redissolving, dialyzing and purifying to obtain the coconut globulin. According to the method, the defects in the prior art are overcome, and the extraction rate of the coconut globulin and the purity of the final product are improved while the good stability and emulsibility of the coconut globulin are ensured.