10056 results about "Freeze dry" patented technology

A freeze dried biocompatible matrix comprising plasma proteins, useful as implants for tissue engineering as well as in biotechnology, and methods of producing the matrix are provided. Mechanical and physical parameters can be controlled by use of auxiliary components or additives which may be removed after the matrix is formed in order to improve the biological properties of the matrix. The matrices according to the present invention may be used clinically per se, or as a cell-bearing implant.

A novel method of forming catalyst particles, on which carbon nanotubes grow based, on a substrate with increased uniformity, and a method of synthesizing carbon nanotubes having improved uniformity are provided. A catalytic metal precursor solution is applied to a substrate. The applied catalytic metal precursor solution is freeze-dried, and then reduced to catalytic metal. The method of forming catalyst particles can minimize agglomeration and/or recrystallization of catalyst particles when forming the catalyst particles by freeze-drying the catalyst metal precursor solution. The catalyst particles formed by the method has a very uniform particle size and are very uniformly distributed on the substrate.

The invention discloses a preparation method of plant enzyme food, belonging to the field of food processing technology. The method comprises the following steps of: performing twice fermentation and compounding on the natural plant raw materials of fresh fruits, vegetables, mushrooms, medicines, grains and flowers to obtain the secondary-fermentation stock solution or raw sauce, and storing; filling and sealing the secondary-fermentation stock solution or raw sauce to obtain liquid or sauce-type plant enzyme food or compound plant enzyme food; or performing low-temperature concentration and freeze drying to obtain solid plant enzyme food or compound plant enzyme food. Due the adoption of the preparation method, the obtained natural plant enzyme food has the functions of improving the immunity of human body, activating cells, promoting metabolism and the like.

A lyophilization process which comprises dissolving a material in one or more solvents for said material to form a solution; forcing said material at least partially out of solution by combining the solution and a non-solvent for the material, which non-solvent is miscible with the solvent or solvents used and wherein said non-solvent is volatilizable under freeze-drying conditions. In addition, for hydrophobic and/or lipophilic materials, the anti-solvent can be omitted, and the solution of the material in the solvent can be subjected directly to freeze drying. The lyophilizates can then be reconstituted with typical aqueous diluent in the case of hydrophilic materials. Hydrophobic and or lipophilic materials can be initially reconstituted with propylene glycol and/or polyethyleneglycol to form a high concentration solution therein and this is further diluted for use with a diluent of Intralipid, plasma, serum, or even whole blood.

Freeze-dried disintegrating tablets, said tablets containing at least a therapeutic agent, a matrix forming agent and a binding agent, in which the tablets contain more than 20% by weight of a matrix forming agent selected from the group consisting of maltodextrins having a DE value between 12 and 40, isomalt and mixtures thereof, the weight ratio between said matrix forming agent and the binding agent being comprised between 2:1 and 50:1.

The present invention provides freeze-dried compositions that include an extract of human feces and a cryoprotectant, and methods for making and using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and/or iatrogenic condition of the colon.

A readily-soluble freeze-dried solid preparation of hGH with a minimal content of degradation products in terms of deamidation, dimers, polymers, and sulphoxide forms, obtainable by a method comprising a single lyophilization of an aqueous slurry of an amorphous hGH isoprecipitate, the slurry having a pH of from about 4.7 to 5.0 and being essentially free of buffer components other than acetate.

A composition comprising a collagen protein and demineralized bone matrix is described wherein the composition is chemically cross-linked with a carbodiimide such as N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC). The crosslinking reaction can be conducted in the presence of N-hydroxysuccinimide (NHS). The collagen can be in a porous matrix or scaffolding. The DBM can be in the form of particles dispersed in the collagen. A method of making the composition is also described wherein a collagen slurry is cast into the desired shape, freeze dried to form a porous scaffolding and infitrated with a solution comprising the cross-linking agent. The composition can be used as an implant for tissue (e.g., soft tissue or bone) engineering.

The present invention discloses a natural fruit and vegetable ferment beverage, and a preparation method thereof. The preparation method for the ferment beverage comprises: adding freshly-squeezed fruit and vegetable juice to marine fish skin collagen peptide; adopting the resulting mixture as a fermentation substrate; carrying out dilution, slurry mixing and sterilization, and then adopting a direct vat and freeze-dried type lactic acid starter to carry out fermentation; stopping the fermentation after 7-11 days until the pH value is 4.3; adding a supplementary feed and carrying out sterilization; then adding high-activity dried yeast to carry out fermentation; stopping the fermentation after 5-7 days until the pH value is 4.0; after storing the fermentation broth at the low temperature, carrying out homogenization, concentration, centrifugation, blending, sterilization and bulking to finally prepare the novel natural fruit and vegetable ferment beverage. According to the ferment beverage of the present invention, the stepwise fermentation is adopted for the freshly-squeezed fruit and vegetable juice by using the lactic acid bacteria and the yeast; the marine fish skin collagen peptide is added to promote the growth and the metabolism of fermentation bacteria, such that metabolic products of nutritional ingredients in the natural fruits and vegetables are rich so as to provide a beneficial nutrition and health effect.

The composition and method for healing tissues is a medicinal composition for facilitating the growth, protection and healing of tissues and cells in animals and humans. The composition is formulated as a either a powder, gel, paste, film, fluid injectable, rehydratable freeze-dried paste or sponge, sprayable solution, topically applied patch with adhesive and reservoir system, an intermediate for coatables such as films and bandages, a matrix for membranes, or as a matrix of flexible polymer(s), or delivered as either an orally ingestible liquid, tablet or capsule. The main ingredient of the formulated compositions is hydrolyzed collagen, which can be combined with polysulfated glycosaminoglycans, hyaluronic acid or salts thereof, or a glucosamine salt, and mixtures thereof. The composition may be formulated as an aqueous eye drop solution.

The invention relates to a kit for measuring the D-Dimer content by adopting a latex immunonephelometry method. The kit comprises a D-Dimer reagent R1, a D-Dimer reagent R2, a D-Dimer diluent and a D-Dimer calibration product, wherein the D-Dimer reagent R1 comprises a buffering solution, a stabilizing agent (1), coagulant and preservative; the D-Dimer reagent R2 comprises mouse anti-human D-Dimer monoclonal antibody latex enhanced particles, a buffering solution, a stabilizing agent (2) and preservative; the D-Dimer diluent comprises a buffering solution and preservative; and the D-Dimer calibration product is prepared by subpacking and freeze-drying a solution comprising a fibrin degradation product D-Dimer, a buffering solution, a stabilizing agent (3), excipient and preservative. The kit has the advantages of simple and rapid operation, accurate quantification, high sensitivity, strong specificity, low detection cost and strong instrument compatibility and is suitable for being popularized and used in various big-scale and small-scale hospitals.

The present invention comprises the discovery and development of an effective cryoprotectant composition, without containing skim milk or any other animal-derived compounds, to achieve long-term stability of freeze-dried lactic acid bacteria (LAB), at different temperatures, whereby the retention of viability of the freeze-dried LAB after 6 months of storage, preferably after 9 months of storage, more preferably after 12 months of storage is more than 50%. The invention is in the field of producing freeze dried bacteria, in particular Lactic acid bacteria. More in particular, the invention relates to the use of a novel combination of cryoprotectants for increasing the viability of bacteria after freeze drying, improving the texture of the lyofilized cake for easy grinding and improving the long term stability of the freeze dried bacteria at different temperature conditions. The invention further relates to such freeze dried bacteria for use in food industry or in human or animal health applications. More in particular, the invention relates to the increased viability and long-term storage of recombinant bacteria capable of expressing heterologous proteins or peptides and administered to humans or animals for therapeutic or vaccination purposes.

This invention provides methods and compositions to preserve bioactive materials in a dried foam matrix. Methods provide non-boiling foam generation and penetration of preservative agents at temperatures near the phase transition temperature of the membranes.

The invention provides a method for preparing fibrilia carboxylation cellulose nanowhiskers, which is characterized by comprising the following steps: soaking fibrilia powder in sodium hydroxide for processing, then processing the fibrilia powder by a former treating agent and taking out the fibrilia powder to be dried in a vacuum oven to obtain preprocessed fibrilia powder; and placing the preprocessed fibrilia powder in a TEMPO oxidation system for catalytic oxidation to obtain a stable cellulose nanowhiskers suspending liquid after mechanical processing and freeze drying the suspending liquid to obtain the fibrilia carboxylation cellulose nanowhiskers having grain diameters of 3-10 nm. According to the invention, fibrilia carboxylation and nano fibrillation are realized and surfaces of prepared nanocrystalline celluloses have carboxyl functional groups, thus the surfaces generate negative charges, electrostatic repulsion among the negative charges can avoid the reunion of nanoparticles, so that the nanocrystalline celluloses can be well dispersed in water and the obtained nanocrystalline celluloses have excellent uniformity of grain sizes.

The invention relates to a lactobacillus microcapsule, a method for preparing the same, and the use of the same. The lactobacillus microcapsule consists of an outer layer of wall material, a freeze dried protective agent and lactobacilli. The invention also provides the method for preparing the lactobacillus microcapsule and the use of the lactobacillus microcapsule as a feed additive. The lactobacillus microcapsule can effectively protect the lactobacilli in a core material, prolong the survival time of the lactobacilli at room temperature and improve the tolerance of the lactobacilli to the metal ions in the feed; in addition, the lactobacillus microcapsule has good gastric acid resistance and can disintegrate in the intestinal canal quickly to release the lactobacilli, thereby improving the utilization rate of the lactobacilli, balancing the micro-ecological environment in the intestinal canal, suppressing the growth of pathogenic bacteria, protecting the health of the intestinal canals of animals, reducing the incidence rate of the intestinal canals of the animals and the like.

The present invention relates to novel compositions of freeze-dried ginseng berry and other natural health promoting ingredients in a mixture suitable for making tea. The compositions and methods of preferred embodiments of the present invention provide a natural vitamin containing composition for making a tea beverage which can be consumed hot or cold and which provides the consumer with a refreshing, delicious, stimulating and healthful experience.

The composite spongy biological dressing contains chitosan, collagen and calcium alginate with weigh tmixing ratio o f 0.5-8:0.5-8:0.1-8. Its preparation method includes the following steps: selecting chitosan and collagen type I, adding calcium alginate, compounding and cross-linking, using buffer solution to make neutralization, emulsifying, prefreezing and one-step freeze-drying so as to obtain the invented dressing with good biological compatibility and strong adhesion property. Said invented dressing possesses active function of promoting wound healing and hemostatic action, can be combined with anti-bacterial medicine to obtain gene engineeirng dressing for curing wound surface infection, also can be combined with active growth factor or active cell to form gene engineering dressingfor curing intractable ulcer and burn wound surface.

Techniques, mixtures, mixing and delivery kits, and improved delivery instruments for implantation of micronized allograft tissue over a microfractured defect. Allograft cartilage tissue is delivered over a cartilage defect that has been debrided and microfractured, without the need for a periosteal covering or separate type of patch sewn over the top. The allograft tissue may be any micronized cartilage particulates obtained by various methods, for example, cartilage delivered in its native form, dehydrated via lyophilization, “freeze-dried,” dehydrated via desiccation, or dehydrated by any other method.

A method for producing porous structures in which a liquid or pasty mixture of substances is brought at least partially to solidification and subsequently freeze-dried.

The invention discloses a biomedical material and a product thereof. The biomedical material has main characteristic that a compound having a catechol group structure is grafted on chitosan to form a chitosan biomedical material having the catechol group structure; or the grafted chitosan is cross-linked to prepare a novel macromolecular biomedical material. The catechol has cell adhesion, so that tissue adhesiveness of the chitosan can be changed, and blood cells can be adsorbed on the material surface to achieve better bleeding-steeping effect. By virtue of the mediation, performance of the chitosan is improved, and tissue affinity is improved. The material is prepared into a novel bleeding-stopping sponge by freeze-drying, and prepared into novel dressing by spinning or prepared into powder dressing by reverse flocculating.

A vessel receives a liquid material, which is freeze-dried in situ within the vessel. A gas permeable material carried by the vessel provides gas transport for sublimation during drying. After freeze drying, an oxygen-free inert gas is introduced into the vessel through the gas permeable material, to occupy the interior of the vessel with the freeze-dried material. The gas permeable material is covered, to trap the oxygen-free inert gas within the vessel with the freeze-dried material. The freeze-dried material is stored in the entrapped oxygen-free inert gas within the vessel for a storage period. The vessel receives a reconstituting liquid for mixing with the freeze-dried material, and conveys the reconstituted freeze-dried material from the vessel for administration to an individual.

The invention relates to a drink, in particular to a probiotic type fruit and vegetable enzyme solid drink. The probiotic type fruit and vegetable enzyme solid drink is prepared from raw materials in parts by mass as follows: 10-50 parts of prebiotics, 5-40 parts of water-soluble dietary fiber, 0-20 parts of sugar alcohol, 0.1-15 parts of fruit powder, 0.1-10 parts of fruit and vegetable enzyme powder, 0.01-15 parts of freeze-dried bifidobacterium infantis powder, 0.01-20 parts of freeze-dried bifidobacterium breve powder, 0.01-30 parts of freeze-dried Lactobacillus plantarum powder, 0.01-25 parts of freeze-dried bifidobacterium longum powder and 0.01-20 parts of freeze-dried Lactobacillus casei powder. The probiotic type fruit and vegetable enzyme solid drink adopts the natural raw materials and the scientific formula and is balanced in nutrition and beneficial to intestinal health and body immunity.

A stabilized human monoclonal antibody preparation containing 1 to 20 mg of D-mannitol per 1 mg of a human monoclonal antibody. This preparation is excellent in stability in a solution state, a freeze drying state and a freezing state, particularly stability against aggregation and precipitation of the human monoclonal antibody at the time of redissolution after freeze drying.

A preparation method for porous microspheres of a biodegradable polymer is provided. The method comprises the following steps: (a) dissolving a biodegradable polymer in an organic solvent to form an oil phase of 1-30% (g/ml) concentration; (b) selecting a pore-forming agent 1-50% of the biodegradable polymer in the step (a) and dissolving the pore-forming agent in water to form an internal water phase; (c) adding dropwise the internal water phase into the oil phase at a volume ratio of (1-30):100, and stirring to form a primary emulsion; (d) preparing a polyvinyl alcohol aqueous solution of 0.5-10% (g/ml) concentration, which serves as an external water phase; (e) pouring the primary emulsion into the external water phase at a volume ratio of (3-30):100, stirring or performing ultrasonic treatment to form a double-emulsion, and allowing the polymer in the double-emulsion to solidify, thereby forming microspheres; (f) freeze-drying the microspheres to obtain the final product. The obtained porous microspheres of the biodegradable polymer have high porosity, large specific surface area and good adsorption and encapsulation properties; and are used for the adsorption and encapsulation of unstable protein drugs, polypeptide drugs and growth factors and can retain pharmaceutical activity and effectiveness thereof.

A method and apparatus for producing particles, the apparatus includes a solution source that supplies a solution, and a fluid source that supplies a fluid. The solution includes a solvent and a solute. A mixer receives the solution and the fluid from the sources, and mixes the solution and the fluid together to form a mixture. The mixture is supplied from the mixer to an expansion assembly at first pressure. The expansion assembly sprays and expands the mixture substantially simultaneously to form frozen droplets, and preferably to form a low-density powder of frozen droplets. A freeze-dry apparatus sublimes the solvent from the particles. A high mass-transfer rate and a uniform open-structure of the powder bed enhances the freeze-drying process. Solid particles having a controlled size distribution are obtained. The particles preferably have a hollow or porous morphology suitable for differing drug delivery applications to include aerosol formulations.

Tissue repair compositions, particularly bone repair compositions, containing (a) bone fragments and (b) homogenized connective tissue, and methods for making the same are provided. Some of the inventive tissue repair compositions contain a radioprotectant. The compositions can be used in the form of an injectable gel, an injectable paste, a paste, a putty, or a rehydratable freeze-dried form. Kits for using such tissue repair compositions are also provided.

A process for lyophilization or freeze-drying of a pharmaceutical product is provided and a liquid formulation suitable for lyophilization. In particular, a process for lyophilization or freeze-drying a liquid formulation that includes a protein active agent, a bulking agent and a saccharide stabilizing agent is provided. The saccharide to bulking agent ratio and the protein concentration of the formulation are important factors that affect crystallization of the bulking agent during lyophilization and storage as are some processing conditions. In one embodiment, the saccharide is a disaccharide, such as sucrose and the crystalline bulking agent is mannitol. The protein can be an antibody or a non-antibody protein.

The invention discloses a graphene-based nano iron oxide composite material and a preparation method thereof. By the method, iron oxide nano granules with uniform diameter and controllable shape and components can be loaded on graphene. The method comprises the following steps of: uniformly dispersing graphite oxide into deionized water by adopting an ultrasonic method to obtain graphene oxide solution; adding an iron salt precursor into the obtained solution and mixing the precursor and the solution uniformly, adjusting the pH of the solution, and hydrolyzing the iron salt; putting the mixed solution into a reaction kettle and performing hydrothermal reaction; and finally, cleaning and freeze-drying the obtained product to obtain the graphene-based nano iron oxide composite material. The raw materials are common and easily obtained, the cost is low, and the preparation process is simple, safe and environmentally-friendly; the prepared graphene-based nano iron oxide composite material has good structural stability and monodispersity; when the composite material is used as a lithium iron battery electrode material, the charge/discharge capacity can reach over 1,000mAh/g; and the composite material has good multiplying power performance and cycle life.

The invention discloses a freeze dried injection for restoring pulse beat which is prepared from the extracted significant portions of gen-seng, ophiopogon root and schisandra fruit. The invention also discloses the process for its preparation, which features in the complete extraction of the active ingredient of the used medicinal materials and preserves the polysaccharide component. The freeze powder and injection preparation has very good protecting action for myocardial ischemia.

The invention discloses a transparent high-strength fiber material. The preparation method comprises the following steps that: cellulose is dissolved in NaOH-urea aqueous solution or LiOH-urea aqueous solution which is pre-cooled at low temperature to obtain a cellulose solution, and different thicknesses of cellulose hydrogels are prepared by the cellulose solution; water in the cellulose hydrogel is replaced into an organic solvent to obtain a cellulose organic gel; the cellulose hydrogel or the cellulose organic gel is subject to supercritical drying, ambient pressure drying or freeze drying to remove a liquid medium so as to obtain a cellulose aerogel; and the cellulose hydrogel, the organic gel or the aerogel are autoclaved at a temperature of 30-200 DEG C under the pressure of 10-160Mpa to obtain the high-strength transparent cellulose material. The cellulose material prepared by the invention has excellent mechanical properties, thermal stability and optical permeability.