469 results about "Keratinocyte" patented technology

The invention belongs to the technical field of preparation of microorganisms and cosmetics, and particularly relates to a strain composition, radix paeoniae rubra fermentation liquor prepared from the strain composition and application of the strain composition in cosmetics. Specifically, Pseudoalteromonas sp. SE9 and Schizoscharomyces pombe JM2-16 are utilized to perform fermentation treatment on radix paeoniae rubra root powder, and the finally obtained radix paeoniae rubra fermentation liquor has multiple effects of resisting oxidation, promoting proliferation and migration of keratinocytes, promoting skin injury repair, resisting allergy, resisting inflammation, relieving and the like, is high in safety, can be used as a cosmetic component, and can be used as a cosmetic preparation. Therefore, the method has good practical application value.

The invention belongs to the technical field of protein engineering and biology, and discloses a recombinant III-type humanized collagen, a coding gene thereof, a protein preparation, a preparation method of the protein preparation, application of the protein preparation and a skin care product. The amino acid sequence of the recombinant III type humanized collagen is as shown in SEQ ID NO. 1. According to the recombinant III type humanized collagen, the transdermal absorption efficiency is greatly improved, and meanwhile, the proliferation and migration capabilities of keratinocytes are also remarkably improved. The collagen is widely applied to the fields of medical cosmetic plastic surgery, cosmetics and the like, and has a good application prospect.

Methods are provided for the cell-based delivery of collagen VII for the treatment of Epidermolysis Bullosa and corneal erosion. The disclosure also provides a composition and a pharmaceutical composition comprises, comprise, or alternatively consist essentially of, or yet further consist of a keratinocyte sheet or a corneal cell sheet.

Provided are a whitening and freckle-removing composition, a gel, a preparation method therefor, and a use thereof, relating to the technical field of skin-care products. The whitening and freckle-removing composition comprises tranexamic acid, nicotinamide, 4-butylresorcinol, licorice extract, 3-o-ethyl ascorbic acid, and glutathione. For the whitening composition, by inhibiting the activity of tyrosinase, blocking the transfer of melanin from melanocytes to keratinocytes, and inhibiting the formation and accumulation of melanin by means of multiple mechanisms including oxidation resistance, and by means of the synergistic action of all components, cosmetic benefits of whitening and freckle-removing are achieved. The whitening and freckle-removing gel has a simple preparation method, has a mild and non-sensitizing formula, is safe and non-irritating, and is suitable for large-scale production and application.

The invention belongs to the technical field of biological medicine, and particularly relates to application of polypeptide in treatment of psoriasis. The amino acid sequence of the polypeptide provided by the invention is SEQ ID NO.1, so that the spleen-body ratio of a psoriasis mouse can be effectively reduced, and the inflammation level in the body can be reduced; the epidermal thickness of psoriasis mice and inflammatory factors in skin lesion tissues can be remarkably reduced, keratinocyte proliferation is reduced, and inflammatory factors released by inflammatory cells recruited to skin lesion positions are reduced. The polypeptide provided by the invention shows certain advantages in the aspects of relieving inflammatory response, promoting tissue repair and the like when being used for treating psoriasis. The invention provides a new method and means for treating psoriasis, and promotes the progress and development of medicine.

The invention provides a whole cortical action compound peptide, which comprises acetyl tetrapeptide-11, hexapeptide-9, palmitoyl hexapeptide-12, palmitoyl hexapeptide-14 and / or acetyl hexapeptide-1, and is characterized in that the whole cortical action compound peptide comprises acetyl tetrapeptide-11, hexapeptide-9, palmitoyl hexapeptide-12, palmitoyl hexapeptide-14 and / or acetyl hexapeptide-1; the compound peptide acts on epidermal basal layer keratinocytes, true epidermal junction (DEJ) structural proteins and / or dermal layer fibroblasts, and synergistically exerts a whole-cortex skin anti-aging effect on epidermal layer, basement membrane, dermal layer and / or nerve fibers. The compound peptide has the advantages of full-cortex three-dimensional anti-aging, layered precise targeting, multi-peptide scientific compounding, matrix synergy, clear action mechanism, efficient growth promotion of seven key proteins, remodeling of a true epidermis structure, low-concentration effect, mildness, no stimulation and the like, and is an advanced anti-aging scheme under a precise skin care trend.

The invention relates to application of Bergapten or an isomer thereof in preparation of a product for improving skin aging and / or promoting skin repair. A large number of experiments prove that Bergapten or an isomer thereof can improve skin aging and / or promote skin repair and has good safety, and the specific expression is as follows: Bergapten can activate expression of SIRT1 protein and is superior to that of an existing activator resveratrol; the Bergapten can obviously inhibit the reduction of the content of various collagens in skin keratinocytes caused by UV light aging stimulation; the Bergapten can be used for promoting the healing capability of cell scratches. In conclusion, the Bergapten or the isomer thereof has great clinical application prospect and value in preparation of products for improving skin aging and / or promoting skin repair.

The invention discloses a metagen prepared by combining saliva with lactobacillus and capable of improving expression of host antibacterial peptide and immune globulin and application of the metagen, and belongs to the technical field of microorganisms. The combined lactobacillus salivarius disclosed by the invention has the capability of regulating and controlling host immunity and improving pathogenic bacterium infection resistance of a host oral cavity, can remarkably up-regulate expression of a human oral keratinocyte antibacterial peptide HBD-2 gene and protein in vitro, and can remarkably up-regulate expression of DEFB3 (homolog of human HBD-2) and secretory immune globulin SIgA in a mouse body; the compound can be used for preparing a series of products for enhancing the oral immunity of a host and resisting oral pathogenic bacteria infection, and has a huge application prospect.

The invention discloses composite gel with ROS and pH dual responsiveness and a preparation method and application thereof.The preparation method comprises the steps that gold nanoparticles are prepared, gold nanostars GNS grow, a surfactant and biguanide drugs are added to obtain drug-loaded gold nanostars GM, a metal organic framework is introduced to obtain drug-loaded nanoparticles GMZ of a core-shell structure, and the composite gel is prepared. And reacting with a TSPBA solution, an astaxanthin solution and a polyvinyl alcohol PVA solution for crosslinking to obtain the ROS and pH dual responsive composite gel. According to the invention, the gold nanostars are used as a photosensitizer to construct the multifunctional composite gel, and the metal organic framework structure degrades under a slightly acidic condition of a psoriasis epithelium microenvironment to release released zinc ions, so that the gel is endowed with various treatment effects, excellent photo-thermal treatment performance and controlled release performance of the zinc ions and drugs. The gel has ROS responsiveness, can remove excessive ROS in a psoriasis microenvironment, and realizes the treatment of oxidation resistance, inflammation resistance and keratinocyte proliferation inhibition of psoriasis by combining with a nano drug delivery system.

The present invention belongs to the technical field of skin care, and discloses a skin repair composition and a skin care product, wherein the skin repair composition comprises the following components by weight percentage: 30-50% wheat germ oil, 1-5% ceramide 3, 1-10% sodium hyaluronate, and 40-60% bifid yeast fermentation product lysate. The present invention utilizes bifid yeast fermentation product lysate to help restore the activity of fibroblasts and keratinocytes, repair cell damage and protect the components in the extracellular matrix ECM, and further strengthens the damage repair speed of ECM by compounding sodium hyaluronate and ceramide, helps cells and extracellular matrix maintain a healthy state and quickly repair damage, and helps strengthen intercellular lipids and cell adhesion at the same time, and finally helps form a good skin surface sebum film through the synergistic effect of wheat germ oil and sodium hyaluronate, and the four cooperate with each other to significantly improve the skin repair effect.

The invention belongs to the technical field of cosmetics, and particularly relates to a natural-source composition with a function of inhibiting multiple types of matrix metalloproteinases of skin and application of the natural-source composition. The composition is prepared from the following components: herba cistanche powder, American ginseng powder, a red-knees herb extract and a creeping rostellularia herb extract. The composition has the effects of inhibiting expression of multiple types of matrix metalloproteinases in skin immortalized keratinocytes, improving elastin synthesis and inhibiting elastase decomposition, and has no obvious side effects. The composition disclosed by the invention can be used for preparing various cosmetics, functional foods and health-care products related to skin aging resistance, and has a wide application prospect.

The invention relates to an application of D-camphor and cymenin in preparation of an anti-inflammatory soothing preparation for sensitive skin. The invention discloses an application of D-camphor and cymenin as only two effective components in preparation of an anti-inflammatory soothing preparation for sensitive skin. The invention further discloses an anti-inflammatory soothing preparation for sensitive skin, only two effective components of the anti-inflammatory soothing preparation for sensitive skin are D-camphor and cystin, and the anti-inflammatory soothing preparation for sensitive skin can reduce the reaction of IL-6, IL-8 and NO related microinflammation in a keratinocyte inflammation model. The invention has the following beneficial effects: 1, in a high proportion window (5-10: 1) of D-camphor and polycymenin, the inhibition of the compound system of D-camphor and polycymenin on IL-6, IL-8 and NO is superior to that of any monomer; 2, stable dispersion and good use experience can be realized through the micro-emulsion type emulsifying essence or the face cream carrier, and meanwhile, the face cream still has feasibility under the conditions of no essence and no conventional preservative.

The invention provides a glycyrrhizic acid coated cryptotanshinone nano-micelle as well as a preparation method and application thereof, and relates to the technical field of skin care products. According to the glycyrrhizic acid-coated cryptotanshinone nano-micelle, glycyrrhizic acid is used as a carrier for carrying cryptotanshinone, and glycyrrhizic acid and cryptotanshinone cooperate with each other to jointly play antibacterial, anti-inflammatory and anti-lipid secretion roles, so that the glycyrrhizic acid-coated cryptotanshinone nano-micelle has an obvious synergistic effect; meanwhile, through preparation of the nano-micelle, the problems that existing cryptotanshinone is poor in water solubility and stability and difficult to apply to a water system cosmetic formula are effectively relieved. In addition, detection verifies that compared with single use of cryptotanshinone, the glycyrrhizic acid coated cryptotanshinone nano-micelle can effectively reduce the toxicity of cryptotanshinone to keratinocytes, can intervene in various main pathogenesis of acne, and has antibacterial, acne-removing and repairing effects.

The invention discloses a composition with sensitive skin repairing and anti-aging functions as well as an emulsion and application of the composition, and belongs to the technical field of cosmetics. The composition specifically comprises a pseudoalteromonas fermentation product extract, collagen, palmitoyl tripeptide-5, acetylchitosamine, sodium hyaluronate, silanetriol, caffeine and acetyl hexapeptide-8, and can promote the generation of laminin-5 and integrin beta1, promote the vitality of keratinocytes and fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, and promote the growth of the keratinocytes and the fibroblasts. The generation of inflammatory factors (IL-6) is inhibited; the generation of collagen IV and collagen VII is promoted; the invention further provides an emulsion prepared from the composition, and the optimal formula of the emulsion is obtained through optimization. The active composition disclosed by the invention has a wide application prospect in the field of cosmetics, and particularly has important significance in sensitive skin repair and anti-aging directions.

The invention relates to the technical field of medical gel, and particularly discloses a sustained-release gel preparation for endometrial repair and a preparation method of the sustained-release gel preparation. Keratinocyte factor sustained-release microspheres containing keratinocyte factor-glucan particles and lactic acid-glycolic acid copolymer shells are constructed around keratinocyte factors, and the keratinocyte factor sustained-release microspheres are loaded by hyaluronic acid hydrogel containing polylysine, collagen and silk fibroin to form the sustained-release gel preparation for endometrial repair. The sustained-release gel preparation has the effect of promoting proliferation of human endometrial cells, can repair endometrial injury, promote injury healing and prevent uterine cavity adhesion, and has important significance in treating endometrial injury and improving physical and psychological health of women.

The invention belongs to the field of daily chemicals, and discloses a penetration enhancing composition which comprises betaine and isosorbide dimethyl ether, the weight ratio of the betaine to the isosorbide dimethyl ether is (20-30): (15-25). The composition is more excellent in permeation enhancing effect, and the combined permeation enhancing mechanism of the composition is that the isosorbide dimethyl ether can temporarily open lipids between adjacent cells by influencing lipids of a stable structure between keratinocytes, and meanwhile, a hydrophilic part in the structure can conveniently help permeation of water-soluble effective components, so that the combined permeation enhancing effect of the composition is improved. Therefore, the absorption effect of the skin is improved. The betaine effectively increases the skin hydration degree, and when the skin water content is increased, membrane pores are enlarged, the tissue tightness is reduced, and the permeation and absorption of active ingredients are increased. Meanwhile, the invention further discloses application of the composition and cosmetics.

The present invention belongs to the technical field of cosmetics, and discloses a composition with moisturizing and repairing effects, a preparation method thereof and an application thereof. The composition of the present invention comprises a xylitol moisturizer, 4D hyaluronic acid, a peach resin extract and hydroxyphenyl propionamide benzoic acid, the xylitol moisturizer comprises at least one of xylitol, dehydrated xylitol and xylitol glucoside, and the 4D hyaluronic acid comprises at least one of sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate and sodium hyaluronate cross-linked polymer. The present invention rationally compounds the xylitol moisturizer, 4D hyaluronic acid, peach resin extract and hydroxyphenyl propionamide benzoic acid, can locate the skin at different layers, effectively increase the moisture content of the epidermis and dermis, and maintain the hydration state of the skin, while repairing damaged keratinocytes, enhancing the barrier function of the skin, and reducing the loss of water, thereby having good moisturizing and repairing effects.

The invention relates to a method for forming a hair follicle-like structure by co-culturing an immortalized hair papilla cell line derived from human hair follicles and keratinocytes. Specifically disclosed is a hair papilla cell line (SV40T-hTERT DPC) which is immortalized by forced expression of SV40T, which deactivates a cancer suppressor gene, in human hair follicle-derived hair papilla cells and hTERT gene, which is a telomerase elongase promoting gene, and keratinocytes, are co-cultured three-dimensionally. From the result, it has been confirmed that a hair follicle-like structure is formed in which a cuticle grows so that a hair follicle analogue in combination with a primary-cuticle hair follicle-derived cell has a shape similar to that of the primary-cuticle hair follicle-derived cell. Therefore, the method can be applied to multiple fields of animal replacement experiments, efficacy evaluation of materials for preventing alopecia / promoting hair follicle growth and the like. Through immortalized hair papilla cells, higher result consistency can be ensured compared with that in the prior art, and stable hair follicle-derived cell supply can be realized, so that the problem of high entry barriers of efficacy evaluation of alopecia materials based on cells is expected to be solved.

The invention relates to the field of cosmetics, in particular to dendrobium devonianum stem extract as well as a preparation method and application thereof. The invention provides a dendrobium devonianum stem extract. The mass ratio of glucose to mannose in the dendrobium devonianum stem extract is 3: 7. The invention provides the dendrobium devonianum stem extract with tightening and moisturizing functions, and the extract can effectively inhibit ROS secreted by UVB-induced in-vitro keratinocytes at a lower concentration, and has a certain tightening effect; the AQP3 level in keratinocytes is improved, the AQP3 gene expression is up-regulated, and a certain moisturizing effect is achieved; the dendrobium devonianum stem extract is used for a formula human body efficacy test, and it is proved that 0.1% of the dendrobium devonianum stem extract has certain tightening and moisturizing effects.

The invention discloses lactobacillus reuteri CCFM1403 with a skin repairing effect and a metagen of the lactobacillus reuteri CCFM1403, and belongs to the technical field of microorganisms and medicines. The lactobacillus reuteri CCFM1403 provided by the invention has the effect of improving the barrier function of the skin in two aspects of external use and oral administration, and is specifically shown in that when the thallus lysate of the lactobacillus reuteri CCFM1403 is externally used, the cell viability and the related barrier function of keratinocytes after injury and the expression of a structural protein gene level can be improved; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of TEWL in apparent aspect, water content of cuticle, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the lactobacillus reuteri CCFM1403. Therefore, the lactobacillus reuteri has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention discloses a metagen which is prepared from bifidobacterium adolescentis CCFM1404 and has the effect of improving skin health, and belongs to the technical field of microorganisms and medicines. The metagen (especially the thallus lysate) of the bifidobacterium adolescentis CCFM1404, provided by the invention, has a good skin barrier repairing effect, which is specifically shown in that the cell viability of keratinocytes after injury and the expression of FLG, IVL and LOR gene levels can be improved by externally applying the thallus lysate of the bifidobacterium adolescentis CCFM1404; the DNFB-induced mouse skin barrier injury can be relieved in the aspects of apparent TEWL and epidermis thickness, up-regulation of epidermis and basal layer protein and the like by oral administration of metabiotics (including thallus lysate and fermentation supernatant) of bifidobacterium adolescentis. Therefore, the bifidobacterium adolescentis has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention discloses an anti-aging repairing composition as well as a preparation method and application thereof, and particularly belongs to the field of preparations for dressing and makeup. The composition is prepared from the following components: a dendrobium officinale extract, oligopeptide-215, a kangaroo claw extract, an euglena parvata extract and a cymbidium hookerianum extract. The composition provided by the invention has an obvious inhibition effect on inflammatory factors, effectively promotes migration of skin keratinocytes to achieve the effect of repairing the skin, and meanwhile, the composition can promote generation of collagen and elastin fibers to achieve the effects of resisting wrinkles and tightening the skin; the skin external preparation containing the composition provided by the invention has the effects of tightening, resisting wrinkles and repairing.

The invention discloses a hydrogel dressing for treating atopic dermatitis and a preparation method and application thereof, and belongs to the technical field of skin medical materials. The dressing is prepared by taking a glycerol / water / ethanol ternary mixed solvent as a dissolving system, dissolving polyvinyl alcohol, tannic acid and aluminum trichloride to construct a polymer skeleton matrix, and then adding traditional Chinese medicine active ingredients. The preparation does not need complex temperature control curing, the ternary solvent can realize uniform dissolution and stable dispersion of the active ingredients, the effective ingredients are prevented from being damaged by high temperature, the encapsulation efficiency of the traditional Chinese medicine extract is more than 90%, and the medicine can be slowly released for 72 hours. The dressing has a multi-target anti-inflammatory effect, can reduce the level of TNF-alpha at a skin lesion and promote proliferation of keratinocytes, is excellent in tensile and torsional stability, and can be attached to the skin for a long time in a dynamic environment. The preparation method is simple in process, controllable in raw material cost, high in production efficiency, suitable for large-scale production, good in dressing biocompatibility, high in safety, suitable for treatment of atopic dermatitis of different severity degrees and large in clinical transformation potential.

The invention discloses a lactobacillus mucus CCFM1401 for repairing a skin barrier based on multiple target spots and a metagen of the lactobacillus mucus CCFM1401, and belongs to the technical field of microorganisms and medicines. The thallus lysate of the fermented lactobacillus mucus CCFM1401, provided by the invention, can be used for improving the cell viability of damaged keratinocytes and the expression of FLG, LOR, IVL, ZO-1, Occludin and CLDN gene levels in the aspect of external application; and viable bacteria and metabiotics (including thallus lysate and fermentation supernatant) of the fermented lactobacillus mucus CCFM1401 are orally taken, so that TEWL can be reduced, the water content of cuticle can be up-regulated, inflammatory reactions (such as skin histamine and serum IgE) can be reduced, and the skin barrier function and expression of structural protein can be up-regulated. Therefore, the fermented lactobacillus mucilaginosus has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention provides application of epiphyllum callus extract in preparation of cosmetics, and belongs to the technical field of new biological materials. Compared with a common epiphyllum extract, the epiphyllum callus extract provided by the invention is rich in high-content active ingredients, such as kaempferol, general flavone, total polyphenol, total protein and total sugar; the antioxidant skincare effect is achieved, and the removal efficiency of cells on DPPH free radicals can be improved. The skin care effect of promoting ATP synthesis is achieved, ATP synthesis can be remarkably promoted, and energy metabolism of damaged mitochondria is improved. Effective components of epiphyllum calluses are extracted, the effect of the epiphyllum calluses on human immortal keratinocytes is evaluated through cell experiments, it is proved that the epiphyllum calluses have excellent effects of resisting oxidation, resisting inflammation and repairing the keratinocytes, and the epiphyllum calluses are suitable for being used as raw materials to be applied to the cosmetic industry.

The invention discloses a small molecule polypeptide for treating psoriasis and application of a pharmaceutical composition of the small molecule polypeptide. According to the present invention, the small molecule polypeptide S1 capable of stably binding to the cell receptor in the psoriasis skin is screened from the peptide library, the small molecule polypeptide S1 is the cyclic peptide composed of CNAGQRSEC, and the small molecule polypeptide can inhibit the proliferation of human keratinocytes. According to the invention, medically acceptable auxiliary materials (such as a transdermal absorbent, a fat-soluble matrix, an emulsifier, a humectant, a pH regulator and an antioxidant) are further added on the basis of the small molecule polypeptide S1 to prepare the medicine cream, so that the stability and transdermal capacity of the medicine can be remarkably improved, and effective treatment of psoriasis is realized.

The invention belongs to the technical field of biological medicines, and relates to an application of beta-guanidinopropionic acid (3-Guanidinopropionic acid, beta-GPA) in preparation of a medicine for treating chronic inflammatory dermatosis. The invention discovers that the creatine transporter SLC6A8 is remarkably and highly expressed in skin lesion tissues of psoriasis for the first time, and the protein is strongly associated with occurrence and development of chronic inflammatory skin diseases such as psoriasis. The SLC6A8 transporter inhibitor beta-guanidino propionic acid can obviously inhibit the expression level of keratinocyte inflammatory factors, and meanwhile, the imiquimod-induced psoriasis model mouse skin lesion symptom is reversed, so that the beta-guanidino propionic acid has the effect of resisting psoriasis. The invention can provide new therapeutic targets and new drug choices for psoriasis and other chronic inflammatory skin diseases.

The invention discloses a pharmaceutical composition, which comprises chlorogenic acid, ferulic acid and a zeolite imidazole framework material, the mass ratio of chlorogenic acid to ferulic acid is 4: 1-1: 4, and the zeolite imidazole framework material is a Zn-based zeolite imidazole framework material. In the composition, the compatibility of chlorogenic acid and ferulic acid can synergistically regulate the proliferation, migration and apoptosis of keratinocytes, and restore the physiological function imbalance of human skin keratinocytes caused by EGFRIs. The ZIF can efficiently carry chlorogenic acid and ferulic acid together, the nanoscale particle size of the ZIF is beneficial to permeation of the medicine into the skin, and hydroxyl on the surface of the ZIF and ceramide in the skin are subjected to hydrogen-bond interaction to retain the medicine at the focus position. The composition preparation disclosed by the invention is good in moisturizing performance and simple and convenient to use, and can be used for effectively treating skin toxic reactions such as pustule type rash, skin dryness and itching, perionychomycosis, abnormal hair regeneration and the like caused by EGFRIs.

Use of cholesterol sulfate or a derivative thereof in the preparation of a composition for treating atopic dermatitis or alleviating symptoms thereof. Cholesterol sulfate or the derivative thereof can inhibit keratinocytes from generating and releasing TSLP, thereby inhibiting the formation of type II inflammation, and can effectively treat or prevent AD and alleviate the symptoms thereof. Provided is a topical medicament or cosmetic comprising cholesterol sulfate or the derivative thereof.

The present invention relates to a method for producing a skin tissue model, comprising: (a) isolating and culturing fibroblasts and keratinocytes from skin tissue; (b) preparing a bioink composition for a dermal layer and a bioink composition for an epidermal layer, the bioink composition containing the isolated cells; and (c) producing a skin tissue model using the bioink composition for a dermal layer and the bioink composition for an epidermal layer.