The invention discloses a longevity medicated wine and a preparation method thereof; according to traditional Chinese medicine theory, 34 natural Chinese herbal medicines such as polygonatum, ganoderma lucidum, ginseng, eclipta alba, radix polygoni multiflori, radix codonopsis, atractylodes rhizome, pine needle, black sesame seed, longan meat, red dates, wolfberry fruit, glossy privet fruit, radix polygalae, fructus psoraleae, asparagus, sweet scented osmanthus, epimedium, cistanche, white poria cocos, morinda root, eucommia bark, padix rehmanniae Preparata, fructus alpiniae oxyphyllae, the rhizome of chuanxiong, astragalus membranaceus, angelica, poria with hostwood, achyranthes root, safflower, radix paeoniae alba, rhizoma drynariae, bighead atractylodes rhizome and agilawood are put into 50 degree-above high quality rice liquor with the weight of 2-3 times of the total weight of the Chinese herbal medicines; and rock sugar, royal jelly and other auxiliary materials are added for preparation by a traditional process. The longevity medicated wine has the effects of activating blood, replenishing blood, dispersing blood stasis, nourishing heart and liver, tonifying spleen and stomach, moistening hair and strengthening muscles and bones, tonifying yuan yang and benefitting Yin deficiency, nourishing kidney, strengthening waist, tonifying Qi, relaxing body, resting mind, resisting senility, preventing aging, supporting the right and securing the root, enhancing immunity and the like. A variety of material components produced by combination of the medicines can play the ideal effect of treating disease when the people is in disease and prolonging life when the people is disease-free.