Traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1.5 to 2.5 parts of oyster peptide, 1.5 to 2.5 parts of sea cucumber peptide, 25 to 35 parts of herba cistanche, 15 to 25 parts of rhizoma polygonati, 10 to 20 parts of fructus lycii, 8 to 12 parts of cortex eucommiae, 8 to 12 parts of fructus rubi, 10 to 20 parts of radix astragali seu hedysari, 20 to 30 parts of herba epimedii, 4 to 6 parts of radix panacis quinquefolii, 1.5 to 2.5 parts of penis et testis cervi, 8 to 12 parts of flos lonicerae, 5 to 8 parts of silkworm chrysalis, 15 to 20 parts of fructus mori, 4 to 6 parts of herba taraxaci, 5 to 8 parts of eurycoma longifolia and 1.5 to 2.5 parts of carpinacol. The traditional Chinese medicine composition disclosed by the invention is reasonable in compatibility, has the effects of tonifying kidney, strengthening essence and nourishing liver, and can be used for treating sexual hypofunction syndromes such as insufficiency of kidney yang, impotence, spermatorrhea and premature ejaculation. The whole prescription is scientific in compatibility, all medicines are mutually coordinated, and both symptoms and root causes are treated.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of traditional Chinese medicine composition, and particularly relates to a traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, a preparation method and application thereof. BACKGROUND

[0002] In traditional Chinese medicine theory, the relationship between liver and kidney is extremely close, and the core embodiment is that liver and kidney are homologous and essence and blood are homologous. Liver stores blood, and kidney stores essence. The formation of blood depends on the nourishment of kidney essence, and the filling of kidney essence also cannot be separated from the supplement of blood. They are interdependent and interconvertible, and jointly maintain the normal physiological function, reproductive function and aging process of the human body. When liver and kidney are insufficient and essence and blood are deficient, the body is lack of nourishment, and a series of typical symptoms may occur, such as soreness of waist and knees, dizziness and tinnitus, blurred vision, memory decline, early graying of hair, loose teeth, insomnia and dreaminess, cold limbs, and the like. Males may have spermatorrhea, premature ejaculation and infertility, and females may have irregular menstruation, amenorrhea and infertility, which seriously affect the physical and mental health and life quality of patients. With the great change of contemporary social lifestyle, the problem of deficiency of liver and kidney essence and blood is increasingly prominent and presents a high incidence. Long-term mental stress, excessive work pressure, overwork, improper diet, lack of exercise and environmental pollution can all accelerate the consumption of kidney essence and the deficiency of liver blood, leading to the continuous expansion of the scope of people with deficiency of liver and kidney essence and blood, and the gradual youth of the age. At present, for the conditioning and treatment of deficiency of liver and kidney essence and blood, there are various traditional Chinese medicine preparations, health care products and medicinal liquor products on the market. However, the existing products still have many limitations in actual application: first, some prescriptions excessively pursue “all-in-one”, and there are various medicinal materials, which not only increases the production cost and quality control difficulty, but also makes it difficult to fully play the synergistic effect between drugs, and even may cause adverse reactions due to improper compatibility; second, some products focus on single deficiency, such as simple kidney tonification or simple liver nourishment, and fail to reflect the whole conditioning thought of “liver and kidney homologous”, leading to one-sided curative effect and difficulty in fundamentally improving the deficiency of essence and blood.

[0003] Therefore, it is of irreplaceable value to provide a traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood to promote the modernization of traditional Chinese medicine and meet the health needs of multiple levels. SUMMARY

[0004] Therefore, one of the purposes of the present application is to provide a traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood.

[0005] The second purpose of the present application is to provide a preparation method of the traditional Chinese medicine composition.

[0006] The third purpose of the present application is to provide a medicinal liquor for nourishing liver and kidney and benefiting essence and blood.

[0007] The fourth object of the present application is to provide the application of the traditional Chinese medicine composition or the preparation method or the liver and kidney nourishing and essence and blood benefiting wine in the preparation of a liver and kidney nourishing and essence and blood benefiting medicine.

[0008] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions. A traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, comprising the following raw materials in parts by weight: 1.5-2.5 parts of oyster peptide, 1.5-2.5 parts of sea cucumber peptide, 25-35 parts of cistanche, 15-25 parts of rhizoma polygonati, 10-20 parts of medlar, 8-12 parts of eucommia ulmoides, 8-12 parts of raspberry, 10-20 parts of radix astragali, 20-30 parts of herba epimedii, 4-6 parts of American ginseng, 1.5-2.5 parts of deer penis, 8-12 parts of honeysuckle, 5-8 parts of silkworm chrysalis, 15-20 parts of mulberry, 4-6 parts of damiana, 5-8 parts of dongguari and 1.5-2.5 parts of cabinda.

[0009] Preferably, the composition comprises the following raw materials in parts by weight: 1.8-2.2 parts of oyster peptide, 1.8-2.2 parts of sea cucumber peptide, 28-32 parts of cistanche, 18-22 parts of rhizoma polygonati, 12-18 parts of medlar, 9-11 parts of eucommia ulmoides, 9-11 parts of raspberry, 13-17 parts of radix astragali, 22-27 parts of herba epimedii, 4.5-5.5 parts of American ginseng, 1.8-2.2 parts of deer penis, 9-11 parts of honeysuckle, 5.5-7 parts of silkworm chrysalis, 17-19 parts of mulberry, 4.5-5.5 parts of damiana, 5.5-7 parts of dongguari and 1.8-2.2 parts of cabinda.

[0010] Preferably, the composition comprises the following raw materials in parts by weight: 2 parts of oyster peptide, 2 parts of sea cucumber peptide, 30 parts of cistanche, 20 parts of rhizoma polygonati, 15 parts of medlar, 10 parts of eucommia ulmoides, 10 parts of raspberry, 15 parts of radix astragali, 25 parts of herba epimedii, 5 parts of American ginseng, 2 parts of deer penis, 10 parts of honeysuckle, 6 parts of silkworm chrysalis, 18 parts of mulberry, 5 parts of damiana, 6 parts of dongguari and 2 parts of cabinda.

[0011] Preferably, the preparation method of the oyster peptide comprises the following steps: mixing oyster and water at a mass ratio of 1: (6-8), heating to 100℃ and then keeping the temperature for 2.5-3.5 hours for extraction, repeating the extraction for 2-3 times, combining the extraction solutions, and then spraying the concentrated solution to obtain oyster peptide.

[0012] Preferably, the preparation method of the sea cucumber peptide comprises the following steps: grinding fresh sea cucumber after removing the internal organs to obtain sea cucumber slurry, adding protease and performing enzymolysis at 50-55℃ for 3-5 hours to obtain a first enzymolysis solution; adding flavor protease and performing enzymolysis at 50℃ for 1.5 hours to obtain a second enzymolysis solution; adding 4% of activated carbon to the second enzymolysis solution, centrifuging and collecting the supernatant; purifying the supernatant, collecting small molecular peptide solution with a molecular weight of 1000-3000 Da, and then drying to obtain sea cucumber peptide.

[0013] The application further provides a preparation method of the traditional Chinese medicine composition, comprising the following steps: The Cistanche, Rhizoma Polygonati, Fructus Lycii, Eucommia ulmoides, Rubus chingii Hu, Astragalus membranaceus, Herba Epimedii, Panax quinquefolium, Deer penis, Lonicera japonica, silkworm chrysalis, Morus alba, Damiana and Yohimbe are weighed, crushed and mixed to obtain a mixture 1; The mixture 1 is added into water, soaked for 12 hours, then extracted for 3 times after being heated to 55-60 DEG C, and the three times of extraction solutions are collected; the extraction solution is concentrated to a relative density of 1.2-1.4, and dried to obtain an extract 1; The extract 1, oyster peptide and sea cucumber peptide are crushed and mixed to obtain a traditional Chinese medicine extract.

[0014] The application further provides a liver and kidney nourishing and essence and blood benefiting wine, and a preparation method of the liver and kidney nourishing and essence and blood benefiting wine is as follows: The traditional Chinese medicine extract obtained by the preparation method is mixed with wine at a mass ratio of 1:24, soaked and stirred for 48-72 hours, and filtered.

[0015] Preferably, the alcohol content of the wine is 38%-65%.

[0016] The application further provides application of the traditional Chinese medicine composition, the preparation method or the liver and kidney nourishing and essence and blood benefiting wine in preparation of a liver and kidney nourishing and essence and blood benefiting product.

[0017] Preferably, the product promotes the development of sexual organs, delays the weight loss trend of patients with yang deficiency syndrome, and shortens the latency period of penile erection.

[0018] Compared with the prior art, the application has the following beneficial effects: The application provides a traditional Chinese medicine composition for liver and kidney nourishing and essence and blood benefiting, wherein Cistanche, Herba Epimedii, Rubus chingii Hu, silkworm chrysalis, Panax quinquefolium, Damiana and Yohimbe are used as monarch drugs, so that the whole prescription has the effects of liver and kidney nourishing and essence and blood benefiting; Fructus Lycii, Eucommia ulmoides, Rhizoma Polygonati, Astragalus membranaceus, Lonicera japonica and Yohimbe are used as minister drugs, which assist the monarch drugs to strengthen the treatment of main diseases and main symptoms, and treat the combined symptoms; oyster peptide, sea cucumber peptide and Deer penis are used as auxiliary drugs, which assist the monarch drugs and the minister drugs to strengthen the treatment, and can also reduce the toxicity and the drug property of the monarch drugs and the minister drugs; Morus alba is used as a guide drug, which leads the other drugs in the prescription to the disease site. The drugs are mixed, so that the combined force of the drugs can drive away the evil. The whole prescription is reasonably matched, and is used for treating soreness of waist and knees, fatigue, impotence and premature ejaculation, urgent urination, frequent urination, painful urination and dripping urination. The whole prescription is scientifically matched, and the drugs are coordinated with each other, so that the prescription can effectively warm the kidney, tonify yang, strengthen the muscles and bones, increase the survival rate of male sperm, improve the immunity and help sleep, and treat the main and secondary symptoms DETAILED DESCRIPTION

[0019] The application provides a traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, which comprises the following raw materials in parts by weight: 1.5-2.5 parts of oyster peptide, 1.5-2.5 parts of sea cucumber peptide, 25-35 parts of cistanche, 15-25 parts of rhizoma polygonati, 10-20 parts of medlar, 8-12 parts of eucommia ulmoides, 8-12 parts of raspberry, 10-20 parts of astragalus, 20-30 parts of herba epimedii, 4-6 parts of American ginseng, 1.5-2.5 parts of deer penis, 8-12 parts of honeysuckle, 5-8 parts of silkworm chrysalis, 15-20 parts of mulberry, 4-6 parts of damiana, 5-8 parts of kava and 1.5-2.5 parts of kava. Preferably, the composition comprises the following raw materials in parts by weight: 1.8-2.2 parts of oyster peptide, 1.8-2.2 parts of sea cucumber peptide, 28-32 parts of cistanche, 18-22 parts of rhizoma polygonati, 12-18 parts of medlar, 9-11 parts of eucommia ulmoides, 9-11 parts of raspberry, 13-17 parts of astragalus, 22-27 parts of herba epimedii, 4.5-5.5 parts of American ginseng, 1.8-2.2 parts of deer penis, 9-11 parts of honeysuckle, 5.5-7 parts of silkworm chrysalis, 17-19 parts of mulberry, 4.5-5.5 parts of damiana, 5.5-7 parts of kava and 1.8-2.2 parts of kava. More preferably, the composition comprises the following raw materials in parts by weight: 2 parts of oyster peptide, 2 parts of sea cucumber peptide, 30 parts of cistanche, 20 parts of rhizoma polygonati, 15 parts of medlar, 10 parts of eucommia ulmoides, 10 parts of raspberry, 15 parts of astragalus, 25 parts of herba epimedii, 5 parts of American ginseng, 2 parts of deer penis, 10 parts of honeysuckle, 6 parts of silkworm chrysalis, 18 parts of mulberry, 5 parts of damiana, 6 parts of kava and 2 parts of kava. The raw materials are all commercially available products known to those skilled in the art.

[0020] In the traditional Chinese medicine composition, cistanche, herba epimedii, raspberry, silkworm chrysalis, American ginseng, damiana and kava are the main drugs. Cistanche is mainly used for tonifying yang, and can improve the symptoms of kidney deficiency such as soreness and weakness of waist and knees and cold feet, and can supplement the essence and blood needed by the body. Herba epimedii can tonify kidney and strengthen yang, strengthen bones and muscles, dispel wind and dampness, and is used for treating impotence, premature ejaculation, sterility and cold infertility. Raspberry enters liver and kidney channels, fills essence and marrow, dredges kidney qi and enters five zang organs and the life gate. Silkworm chrysalis has the effect of supplementing sex hormones, can increase the weight of prostate, seminal vesicle and sponge ball muscle of adult male castrated mice, and can also protect the liver. American ginseng can tonify qi and nourish yin, clear away heat and generate fluid, regulate qi and blood, and reduce excessive yin fire. Damiana can stimulate male hormones, improve sexual ability, regulate the nervous system, promote related hormones, secrete and defecate, relieve menopausal symptoms, and make people happy. Kava can relieve wetness and remove yellow, improve physical strength and energy, reduce fatigue, and improve male sexual dysfunction. In the application, cistanche, herba epimedii, raspberry, silkworm chrysalis, American ginseng, damiana and kava are the main drugs, which determine the main direction of the composition, and the medicinal effect of the seven drugs is the first in the composition, which can tonify liver and kidney and benefit essence and blood.

[0021] In the traditional Chinese medicine composition of the present application, the Fructus Lycii, Eucommia ulmoides, Rhizoma Polygonati, Radix Astragali, Honeysuckle and Cabinda are the ministerial medicines. The Fructus Lycii nourishes Yin without causing Yin deficiency, and helps Yang without causing Yang deficiency, and is the best product for benefiting essence and eyesight, and can produce essence and benefit Qi, and eliminate internal heat due to Yin deficiency. The Eucommia ulmoides can supplement liver and kidney and strengthen bones and muscles, and can improve the symptoms of soreness and weakness of waist and knees. The Rhizoma Polygonati has the effects of tonifying Qi and nourishing Yin, and can enhance physical strength, relieve fatigue, improve symptoms such as dry mouth and throat, and soreness and weakness of waist and knees. The Radix Astragali can benefit Qi and ascend Yang, and can stop sweating, benefit water and clear edema, and regulate deficiency of Yin and Yang, and can supplement kidney essence, and is the medicine for upper, middle and lower, and internal and external Sanjiao. The Honeysuckle has the effect of resisting pathogenic microorganisms, and the soaking liquid can inhibit Staphylococcus aureus, Staphylococcus albus, Hemolytic Streptococcus, Escherichia coli and Proteus vulgaris, and animal experiments show that it can cause central nervous system excitement in mice and other animals, and the strength of the effect is 1 / 6 of that of caffeine. The Cabinda can relieve fatigue, improve sexual function, and increase physical strength, and is used for improving male sexual dysfunction, reducing anxiety and stress, and helping kidney Yang deficiency. The six medicines are used together as the ministerial medicines of the composition, and assist the monarch medicine to strengthen the treatment of the main disease and symptoms, and enhance the curative effect of the composition.

[0022] In the traditional Chinese medicine composition of the present application, the oyster peptide, sea cucumber peptide and deer penis are the auxiliary medicines. The oyster peptide is obtained by using peptide molecular biological technology in the processing of oysters, and can be generated by enzymolysis, and has the effects of strengthening Yang, detoxification, tonifying Qi and blood, preventing aging and protecting liver. The sea cucumber peptide is extracted from sea cucumbers and small molecule active peptide substances, and is rich in collagen, polysaccharides and saponins, and can help to enhance immunity, promote tissue repair, contain various amino acids, and has the advantages of high trace element and high nutritional value, and can relieve muscle soreness after exercise. The deer penis can supplement kidney essence and strengthen kidney Yang, and strengthen waist and knees. The oyster peptide, sea cucumber peptide and deer penis are used together as the auxiliary medicines, and assist the monarch medicine and the ministerial medicine to strengthen the treatment effect.

[0023] In the traditional Chinese medicine composition of the present application, the mulberry is used as the leading medicine, and can lead the other medicines in the prescription to the disease, and harmonize the medicines to drive away evil. At the same time, the mulberry and the raspberry can supplement liver and kidney together, and have high nutritional value and medicinal value, and have the effects of supplementing liver and protecting kidney and resisting aging.

[0024] The whole prescription is reasonably matched, and the medicines are coordinated with each other, and have the effects of supplementing kidney and strengthening Yang, benefiting essence and strengthening muscles, nourishing liver and eyesight, and regulating menstruation and stopping leucorrhea, and is used for the symptoms of kidney Yang deficiency, impotence, spermatorrhea, premature ejaculation and decreased sexual function. The pharmacological experiments show that the composition can increase the seminal vesicle and the preputial gland of the animals with kidney deficiency, prolong the swimming time of the animals with Yang deficiency, enhance the self-activity ability, and delay the weight loss of the mice with Yang deficiency, and can improve the excitability of the penis of the trend rats to external stimulation.

[0025] In the present application, the preparation method of the oyster peptide preferably comprises the following steps: mixing oysters and water at a mass ratio of 1: (6-8), heating to 100 DEG C and then keeping for 2.5-3.5 h, repeating the extraction for 2-3 times, combining the extraction liquid, concentrating to obtain concentrated extract, and spraying the concentrated extract, preferably controlling the vacuum degree to be 0.06-0.08 MPa and the temperature to be 70 DEG C; the spraying particle size is preferably 80 mesh.

[0026] In the present application, the preparation method of the sea cucumber peptide preferably comprises the following steps: grinding fresh sea cucumber into sea cucumber slurry after removing the internal organs, adding protease, and enzymolysis at 50-55 DEG C for 3-5 h to obtain the first enzymolysis liquid; adding flavor protease and enzymolysis at 50 DEG C for 1.5 h to obtain the second enzymolysis liquid; adding 4% activated carbon to the second enzymolysis liquid, centrifuging, and collecting the supernatant; the supernatant is preferably subjected to ultrafiltration to remove macromolecular impurities to obtain sea cucumber crude extract; and the crude extract is preferably purified to collect small molecular peptide solution with a molecular weight of 1000-3000 Da, and then dried to obtain sea cucumber peptide.

[0027] In the method for preparing sea cucumber peptide, the sea cucumber is preferably ground by a ball mill, and the grinding temperature is preferably 4 DEG C; the particle size of the sea cucumber slurry after grinding is preferably 15-20 μm; the addition amount of the protease is preferably 0.8%-1.2% w / w of the sea cucumber slurry; and the addition amount of the flavor protease is preferably 0.03%-0.5% w / w of the first enzymolysis liquid; the protease and the flavor protease are both purchased from Ningxia Xiasen Industrial Group Co., Ltd.

[0028] The oyster peptide and the sea cucumber peptide prepared by the method have completely retained the original nutrients of oysters and sea cucumbers, such as vitamins, trace elements and taurine, and are more quickly absorbed by the human body than single amino acid or protein, have higher biological titer and more important physiological functions than ordinary oyster and sea cucumber products.

[0029] The present application also provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps: weighing and crushing Cistanche deserticola Y.C.Ma, Polygonatum sibiricum, Lycium barbarum, Eucommia ulmoides, Rubus chingii Hu, Astragalus membranaceus, Herba Epimedii, Panax quinquefolium, deer penis, honeysuckle, silkworm chrysalis, mulberry, damiana and khat, to obtain a mixture 1; adding water to the mixture 1, heating to 55-60 DEG C, and extracting for three times, preferably for 30 min, 25 min and 20 min respectively, collecting the extraction liquid after each extraction, and concentrating the combined extraction liquid to a relative density of 1.2 (50 DEG C); and drying to obtain an extract 1; and crushing the extract 1, oyster peptide and sea cucumber peptide, and mixing them to obtain a traditional Chinese medicine extract.

[0030] In the preparation method of the traditional Chinese medicine composition, the Herba Cistanche, Rhizoma Polygonati, Fructus Lycii, Eucommia ulmoides Oliv., Rubus chingii Hu, Radix Paeoniae Alba, Herba Epimedii, Radix Panacis Quinquefolii, Deer Penis, Honeysuckle, Silkworm Chrysalis, Morus alba, Damiana, East Gelaali and Cabinda are preferably crushed to pass through a 20-mesh sieve; the extract 1, oyster peptide and sea cucumber peptide are respectively crushed to pass through an 80-mesh sieve and then mixed.

[0031] The application further provides a liver and kidney nourishing and essence and blood benefiting wine, and a preparation method of the liver and kidney nourishing and essence and blood benefiting wine is as follows: the traditional Chinese medicine extract obtained by the preparation method is mixed with wine at a mass ratio of 1:24, soaked and stirred for 48-72 h, and then filtered.

[0032] The application further provides application of the traditional Chinese medicine composition, the preparation method or the liver and kidney nourishing and essence and blood benefiting wine in preparation of a liver and kidney nourishing and essence and blood benefiting medicine, and preferably the medicine promotes development of sexual organs, delays weight loss trend of a patient with yang deficiency syndrome, and shortens a penile erection latency period.

[0033] The technical solutions provided by the application are described in detail below in combination with examples, but they should not be understood as limitations to the protection scope of the application.

[0034] Example 1 A liver and kidney nourishing and essence and blood benefiting traditional Chinese medicine composition, and raw materials are as follows in terms of weight fraction: Oyster peptide 1.6 parts, sea cucumber peptide 1.6 parts, Herba Cistanche 33 parts, Rhizoma Polygonati 24 parts, Fructus Lycii 12 parts, Eucommia ulmoides Oliv. 12 parts, Rubus chingii Hu 12 parts, Radix Paeoniae Alba 18 parts, Herba Epimedii 28 parts, Radix Panacis Quinquefolii 6 parts, Deer Penis 1.6 parts, Honeysuckle 8 parts, Silkworm Chrysalis 5 parts, Morus alba 16 parts, Damiana 6 parts, East Gelaali 5 parts and Cabinda 1.6 parts.

[0035] The preparation method of the oyster peptide is as follows: Oyster and water are mixed at a mass ratio of 1:7, heated to 100℃, and then soaked and extracted for 3 h, and the extraction is repeated twice, the extraction liquid is combined, concentrated and sprayed (vacuum degree 0.07 MPa, temperature 70℃), and then oyster peptide is obtained.

[0036] The preparation method of the sea cucumber peptide is as follows: Fresh sea cucumber is removed of internal organs and then ground into sea cucumber slurry using a ball mill at 4℃, the sea cucumber slurry has a particle size of 15-20 μm, protease is added, the protease is added in an amount of 1.1% of the mass of the sea cucumber slurry, and then enzymolysis is carried out at 52℃ for 4 h, and then a first enzymolysis liquid is obtained; Flavour protease is added to the first enzymolysis liquid, the flavour protease is added in an amount of 0.05% of the mass of the first enzymolysis liquid, and then enzymolysis is carried out at 50℃ for 1.5 h, and then a second enzymolysis liquid is obtained; Add 4% activated carbon in the second enzymatic hydrolysate, collect the supernatant after centrifugation (5000 rpm, 15 min); The supernatant is subjected to ultrafiltration to obtain a sea cucumber crude extract, and the sea cucumber crude extract is purified to collect a small molecular peptide solution with a molecular weight of 1000-3000 Da, and vacuum freeze-drying to obtain the sea cucumber peptide.

[0037] The preparation method of the traditional Chinese medicine composition comprises the following steps: The Cistanche deserticola Y.C.Ma, Polygonatum sibiricum, Lycium barbarum, Eucommia ulmoides, Rubus chingii Hu, Astragalus membranaceus, Herba Epimedii, Panax quinquefolium, deer penis, honeysuckle, silkworm chrysalis, mulberry, damiana and cabinda are weighed, respectively crushed (20 mesh sieve) and mixed to obtain a mixture 1; water is added to the mixture 1, the mass ratio of the mixture 1 to water is 1:7, the mixture 1 is soaked at static state at room temperature for 12 hours, and then extracted at 60°C for three times, the first extraction is performed for 30 minutes, the second extraction is performed for 25 minutes, and the third extraction is performed for 20 minutes; the extractive is collected after each extraction, the extractives are combined and concentrated to a relative density of 1.2 (50°C), and then dried to obtain an extract 1; the extract 1, oyster peptide and sea cucumber peptide are respectively crushed (through 80 mesh sieve) and mixed to obtain a traditional Chinese medicine extract.

[0038] Example 2 A traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, the raw materials are as follows in terms of weight fraction: 2 parts of oyster peptide, 2 parts of sea cucumber peptide, 30 parts of Cistanche deserticola Y.C.Ma, 20 parts of Polygonatum sibiricum, 15 parts of Lycium barbarum, 10 parts of Eucommia ulmoides, 10 parts of Rubus chingii Hu, 15 parts of Astragalus membranaceus, 25 parts of Herba Epimedii, 5 parts of Panax quinquefolium, 2 parts of deer penis, 10 parts of honeysuckle, 6 parts of silkworm chrysalis, 18 parts of mulberry, 5 parts of damiana, 6 parts of cabinda and 2 parts of cabinda.

[0039] The preparation method of the oyster peptide, the preparation method of the sea cucumber peptide and the preparation method of the traditional Chinese medicine composition are the same as those in Example 1.

[0040] Example 3 A traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, the raw materials are as follows in terms of weight fraction: 2.4 parts of oyster peptide, 2.4 parts of sea cucumber peptide, 26 parts of Cistanche deserticola Y.C.Ma, 16 parts of Polygonatum sibiricum, 18 parts of Lycium barbarum, 9 parts of Eucommia ulmoides, 9 parts of Rubus chingii Hu, 13 parts of Astragalus membranaceus, 23 parts of Herba Epimedii, 4.5 parts of Panax quinquefolium, 2.4 parts of deer penis, 11 parts of honeysuckle, 6 parts of silkworm chrysalis, 19 parts of mulberry, 4.5 parts of damiana, 7 parts of cabinda and 2.4 parts of cabinda.

[0041] The preparation method of the oyster peptide, the preparation method of the sea cucumber peptide and the preparation method of the traditional Chinese medicine composition are the same as those in Example 1.

[0042] Example 4 A traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, the raw materials are as follows in terms of weight fraction: The traditional Chinese medicine composition obtained in Example 1 is mixed with liquor at a mass ratio of 1:24, soaked and stirred for 60 h, filtered, and the supernatant is collected to prepare the liver and kidney nourishing and essence and blood benefiting liquor.

[0043] In this example, the liquor is a white liquor with an alcohol content of 60%.

[0044] Example 5 A liver and kidney nourishing and essence and blood benefiting liquor is prepared by the following method: The traditional Chinese medicine composition obtained in Example 2 is mixed with liquor at a mass ratio of 1:24, soaked and stirred for 72 h, filtered, and the supernatant is collected to prepare the liver and kidney nourishing and essence and blood benefiting liquor.

[0045] In this example, the liquor is a white liquor with an alcohol content of 50%.

[0046] Example 6 A liver and kidney nourishing and essence and blood benefiting liquor is prepared by the following method: The traditional Chinese medicine composition obtained in Example 3 is mixed with liquor at a mass ratio of 1:24, soaked and stirred for 50 h, filtered, and the supernatant is collected to prepare the liver and kidney nourishing and essence and blood benefiting liquor.

[0047] In this example, the liquor is a white liquor with an alcohol content of 40%.

[0048] Comparative Example 1 A liver and kidney nourishing and essence and blood benefiting traditional Chinese medicine composition differs from Example 1 in that the flavor protease is replaced by pepsin in the preparation method of the sea cucumber peptide: pepsin is added to the first enzyme solution, the amount of pepsin added is 2% of the mass of the first enzyme solution, and the second enzyme solution is obtained by enzymolysis at 40℃ for 1.5 h.

[0049] A liver and kidney nourishing and essence and blood benefiting liquor is prepared by the following method: The traditional Chinese medicine composition obtained in this comparative example is mixed with liquor at a mass ratio of 1:24, soaked and stirred for 60 h, filtered, and the supernatant is collected to prepare the liquor.

[0050] In this comparative example, the liquor is a white liquor with an alcohol content of 60%.

[0051] Comparative Example 2 A liver and kidney nourishing and essence and blood benefiting traditional Chinese medicine composition differs from Example 1 in that the flavor protease is replaced by papain in the preparation method of the sea cucumber peptide: papain is added to the first enzyme solution, the amount of papain added is 0.2% of the mass of the first enzyme solution, and the second enzyme solution is obtained by enzymolysis at 65℃ for 1.5 h.

[0052] A liver and kidney nourishing and essence and blood benefiting liquor is prepared by the following method: The traditional Chinese medicine composition obtained in this comparative example is mixed with liquor at a mass ratio of 1:24, soaked and stirred for 60 h, filtered, and the supernatant is collected to prepare the liquor.

[0053] In the present comparative example, the wine is a white wine with an alcohol content of 60%.

[0054] Comparative Example 3 A traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, which differs from Example 1 in that the flavor protease is replaced by trypsin in the preparation method of the sea cucumber peptide: adding trypsin in the first enzyme solution, the amount of trypsin added is 3% of the mass of the first enzyme solution, and the second enzyme solution is obtained by enzymolysis at 45°C for 1.5h.

[0055] A wine for nourishing liver and kidney and benefiting essence and blood, which is prepared according to the following method: The traditional Chinese medicine composition obtained in the present comparative example is mixed with wine at a mass ratio of 1:24, and after soaking and stirring for 60h, the supernatant is collected by filtration to prepare the medicinal wine.

[0056] In the present comparative example, the wine is a white wine with an alcohol content of 60%.

[0057] Comparative Example 4 A traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, which differs from Example 1 in that the flavor protease is replaced by neutral protease in the preparation method of the sea cucumber peptide: adding neutral protease in the first enzyme solution, the amount of neutral protease added is 3.5% of the mass of the first enzyme solution, and the second enzyme solution is obtained by enzymolysis at 50°C for 1.5h.

[0058] A wine for nourishing liver and kidney and benefiting essence and blood, which is prepared according to the following method: The traditional Chinese medicine composition obtained in the present comparative example is mixed with wine at a mass ratio of 1:24, and after soaking and stirring for 60h, the supernatant is collected by filtration to prepare the medicinal wine.

[0059] In the present comparative example, the wine is a white wine with an alcohol content of 60%.

[0060] Comparative Example 5 A traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, which differs from Example 1 in that the flavor protease is replaced by alkaline protease in the preparation method of the sea cucumber peptide: adding alkaline protease in the first enzyme solution, the amount of alkaline protease added is 0.05% of the mass of the first enzyme solution, and the second enzyme solution is obtained by enzymolysis at 60°C for 1.5h.

[0061] A wine for nourishing liver and kidney and benefiting essence and blood, which is prepared according to the following method: The traditional Chinese medicine composition obtained in the present comparative example is mixed with wine at a mass ratio of 1:24, and after soaking and stirring for 60h, the supernatant is collected by filtration to prepare the medicinal wine.

[0062] In the present comparative example, the wine is a white wine with an alcohol content of 60%.

[0063] Example 7 Sensory evaluation of different wines for nourishing liver and kidney and benefiting essence and blood.

[0064] 15 people were selected to conduct sensory evaluation on the liver and kidney tonifying and essence and blood tonifying wine of Example 4 to Example 6, Comparative Example 1 to Comparative Example 5, and sensory evaluation was conducted using a 9-point scale, and the evaluation content included color (2 points), aroma (2 points), taste (3 points), and mouthfeel (2 points), and the scoring results are shown in Table 1.

[0065] Table 1 Sensory evaluation results

[0066] According to the results in Table 1, the use of flavor protease to prepare sea cucumber peptide has a debittering effect, and the obtained sea cucumber peptide has obvious umami taste. The medicinal liquor configured with the sea cucumber peptide prepared by the application is transparent and colorless, pure sweet and slightly clean, elegant smell, light Chinese medicine flavor, and higher sensory score.

[0067] Example 8 Pharmacological effects of different traditional Chinese medicine compositions.

[0068] 1. Effect on kidney deficiency syndrome.

[0069] 1.1 Materials: traditional Chinese medicine compositions in Example 1 to Example 2; Jingjiu: produced by Hubei Jing Company, production date August 2, 2023; testosterone propionate injection: Shanghai Ninth Pharmaceutical Factory, batch number 202302010.

[0070] Male rats, 98-113g, provided by Henan Animal Center.

[0071] 1.2 Test.

[0072] The rats were anesthetized with sodium pentobarbital (0.6%, 7.5ml / kg), the scrotal skin was disinfected with iodophor, the bilateral testes were removed, and penicillin G (20,000 U / kg) was injected intramuscularly after the operation. 3d after castration, 10 rats in each group were randomly divided into 6 groups: Castration model group: intragastrically administered with normal saline 10ml / kg / d; Testosterone propionate group: intragastrically administered with testosterone propionate injection 2mg / kg / d; Jingjiu group: intragastrically administered with Jingjiu 10ml / kg / d; Example 1: The composition in Example 1 was dissolved in normal saline, and the intragastric amount was 2.48mg / kg / d based on the weight of the composition; Example 2: The composition in Example 2 was dissolved in normal saline, and the intragastric amount was 2.48mg / kg / d based on the weight of the composition; Normal control group: another 10 rats of the same batch were not castrated, and were intragastrically administered with normal saline 10ml / kg / d.

[0073] The rats in each group were fed in the same way. On the 21st day after administration, the animals were weighed and then sacrificed, and the seminal vesicle, prostate and preputial gland were weighed immediately. The results are shown in Table 2.

[0074] Table 2 Effects of different compositions on animal models of kidney deficiency (X ± S)

[0075] Note: # indicates P < 0.01 compared with the castration model group.

[0076] The results show that the body weight and the weight of each reproductive organ of the kidney deficiency animal are significantly lower than those of the normal control group, indicating that the kidney deficiency animal model is successful. The weights of the seminal vesicle, prostate and preputial gland of each animal administered with the composition of the present application are significantly higher than those of the castration model group, and there is a significant difference in statistical processing (P < 0.01), indicating that long-term drinking of the liver-nourishing and eye-opening kidney-tonifying and essence-filling wine has the effect of promoting the development of sexual organs (i.e. the effect of androgen).

[0077] 2. Effect on yang deficiency syndrome.

[0078] 1.1 Materials: Traditional Chinese medicine compositions of Examples 1-2; Jingjiu: produced by Hubei Jing Company, production date August 2, 2023; Hydrocortisone injection: Henan Runhong Pharmaceutical Co., Ltd., batch number 202302010.

[0079] Kunming mice, weighing 20±2g, were provided by the Henan Animal Center.

[0080] 1.2 Test.

[0081] The mice were randomly divided into 5 groups, and each group was treated as follows: Normal control group: intragastrically administered with normal saline 10ml / kg; Yang deficiency syndrome group: intramuscularly injected with hydrocortisone 2.5mg / kg; Jingjiu group: intramuscularly injected with hydrocortisone 2.5mg / kg and intragastrically administered with Jingjiu 10ml / kg; Example 1: The composition in Example 1 was dissolved in normal saline, and the intragastric amount was 2.48mg / kg based on the weight of the composition, and hydrocortisone 2.5mg / kg was injected intramuscularly at the same time; Example 2: The composition in Example 2 was dissolved in normal saline, and the intragastric amount was 2.48mg / kg based on the weight of the composition, and hydrocortisone 2.5mg / kg was injected intramuscularly at the same time.

[0082] One hour after the last administration, the body weight, number of spontaneous activities (within 10 minutes) and low-temperature swimming survival time (water temperature 10°) of the animals in each group were recorded. The results are shown in Table 3.

[0083] Table 3 Effects of different compositions on animal model of yang deficiency (X±S)

[0084] Note: # indicates P<0.05 compared with yang deficiency syndrome group.

[0085] The results show that after the mice are injected with large dose of hydrocortisone for 7 days, the animals appear yang deficiency syndrome performances such as weight loss, reduced activity, slow reaction, and curled arching back. Yang deficiency leads to yang excess and yang deficiency leads to external cold, so yang deficiency syndrome appears cold appearance, which indicates that the model selection in the experiment is successful.

[0086] The experiment shows that: (1) Effects of the composition of the present application on the survival time of yang deficiency syndrome mice in low temperature swimming: large dose of hydrocortisone causes the decrease of the cold tolerance and fatigue tolerance of mice, and the composition of the present application can significantly prolong the survival time of yang deficiency syndrome mice in low temperature swimming, which has significant difference (P<0.05) compared with yang deficiency syndrome group and normal control group. (2) Effects of the composition of the present application on the spontaneous activity of yang deficiency syndrome mice: it can significantly enhance the spontaneous activity of yang deficiency syndrome mice, which has significant difference (P<0.05) compared with yang deficiency syndrome group and normal control group. (3) Effects of the composition of the present application on the body weight of yang deficiency syndrome mice: it can increase the body weight of yang deficiency syndrome mice, and the body weight of the two groups of mice is increased by 2.2g and 2.9g respectively after 7 days of administration, while the body weight of yang deficiency syndrome mice is reduced by 2.6g, which indicates that it has the trend of delaying the weight loss of yang deficiency syndrome mice.

[0087] 3. Yang tonifying experiment 60 male rats with a weight of 98-116g are divided into groups, and the castration operation and the administration dose are the same as those in "1. Effects on kidney deficiency syndrome". On the 21st day after administration, the stimulating electrode of WQ-10B multi-purpose electronic acupoint tester (produced by Beijing Instrument Factory) is placed on the penis of the rats, and the current stimulation intensity is 3mA, and the penile erection (erection latency) from the beginning of stimulation is recorded. The results are shown in Table 4.

[0088] Table 4 Effects of different compositions on the penile erection function of castrated rats (X±S)

[0089] Note: ### indicates P<0.001 compared with the castration model group.

[0090] The results show that the composition of the present application can improve the excitability of the penis of castrated rats to external stimulation, and has the effect of shortening the penile erection latency of rats under the action of local electrical stimulation, which has very significant difference (P<0.001) compared with the castration model group.

[0091] Example 9 Effects of liver and kidney nourishing and essence and blood benefiting wine.

[0092] Select 100 married men, with kidney deficiency caused by waist sore, leg pain, fatigue, impotence symptoms of male patients, randomly divided into experimental and control groups, 50 people in each group. The experimental group drinks 125 mL of liver and kidney nourishing and blood nourishing wine provided by Example 4 every day, and 49 patients show increased sexual desire, enlarged penis, long erection time, and significant improvement in symptoms such as waist sore, leg pain, and fatigue. The control group drinks 125 mL of Hubei Jingpail Co., Ltd. 2023 August production of Jingjiu (38 °) every day, and there is no significant improvement after continuous drinking for 7 days.

[0093] Select 100 married men, with impotence, sexual dysfunction, premature ejaculation, mental depression, and weakness of the limbs, and randomly divide them into experimental and control groups, 50 people in each group. The experimental group drinks 125 mL of liver and kidney nourishing and blood nourishing wine provided by Example 5 every day, and 50 patients have significant improvement: increased sexual excitement and increased sexual frequency. The control group drinks 125 mL of Hubei Jingpail Co., Ltd. 2023 August production of Jingjiu (38 °) every day, and there is no significant improvement after continuous drinking for 7 days.

[0094] Select 100 men aged 60-80 with impotence, sexual dysfunction, premature ejaculation, mental depression, and weakness of the limbs, and randomly divide them into experimental and control groups, 50 people in each group. The experimental group drinks 125 mL of wine provided by Example 6 every day, and there is a significant effect within 7 days. In terms of limb weakness, waist soreness, leg pain, and sexual function, there is a general improvement. The control group drinks 125 mL of Hubei Jingpail Co., Ltd. 2023 August production of Jingjiu (38 °) every day, and there is no significant improvement after continuous drinking for 7 days.

[0095] The above results show that the composition of the present application has good clinical effect, fast onset, and obvious therapeutic effect.

[0096] The above is only a preferred embodiment of the present application, and it should be noted that for ordinary skilled persons in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, and these improvements and refinements should be considered as the protection scope of the present application.

Claims

1. A traditional Chinese medicine composition for nourishing liver and kidney and benefiting essence and blood, characterized in that, By weight, it includes the following ingredients: 1.5-2.5 parts oyster peptide, 1.5-2.5 parts sea cucumber peptide, 25-35 parts Cistanche deserticola, 15-25 parts Polygonatum sibiricum, 10-20 parts wolfberry, 8-12 parts Eucommia ulmoides, 8-12 parts raspberry, 10-20 parts Astragalus membranaceus, 20-30 parts Epimedium brevicornu, 4-6 parts American ginseng, 1.5-2.5 parts deer antler velvet, 8-12 parts honeysuckle, 5-8 parts silkworm pupa, 15-20 parts mulberry, 4-6 parts Datura stramonium, 5-8 parts Tongkat Ali, and 1.5-2.5 parts Cabinda.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, By weight, the ingredients include the following: 1.8-2.2 parts oyster peptide, 1.8-2.2 parts sea cucumber peptide, 28-32 parts Cistanche deserticola, 18-22 parts Polygonatum sibiricum, 12-18 parts Lycium barbarum, 9-11 parts Eucommia ulmoides, 9-11 parts Raspberry, 13-17 parts Astragalus membranaceus, 22-27 parts Epimedium brevicornu, 4.5-5.5 parts American ginseng, 1.8-2.2 parts deer antler velvet, 9-11 parts honeysuckle, 5.5-7 parts silkworm pupa, 17-19 parts mulberry, 4.5-5.5 parts Datura stramonium, 5.5-7 parts Tongkat Ali, and 1.8-2.2 parts Cabinda.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, By weight, the ingredients include the following: 2 parts oyster peptide, 2 parts sea cucumber peptide, 30 parts Cistanche deserticola, 20 parts Polygonatum sibiricum, 15 parts wolfberry, 10 parts Eucommia ulmoides, 10 parts raspberry, 15 parts Astragalus membranaceus, 25 parts Epimedium, 5 parts American ginseng, 2 parts deer antler velvet, 10 parts honeysuckle, 6 parts silkworm pupa, 18 parts mulberry, 5 parts Damochia javanica, 6 parts Tongkat Ali, and 2 parts Cabinda.

4. The traditional Chinese medicine composition according to any one of claims 1-3, characterized in that, The preparation method of the oyster peptide includes the following steps: oysters and water are mixed at a mass ratio of 1:(6~8), heated to 100℃ and kept warm for 2.5~3.5h, the extraction is repeated 2~3 times, the extracts are combined, concentrated and sprayed into powder to obtain oyster peptide.

5. The traditional Chinese medicine composition according to any one of claims 1-3, characterized in that, The preparation method of the sea cucumber peptide includes the following steps: after removing the internal organs of fresh sea cucumber, it is ground into sea cucumber slurry, protease is added, and enzymatic hydrolysis is carried out at 50~55℃ for 3~5h to obtain the first enzymatic hydrolysate; flavor protease is added, and enzymatic hydrolysis is carried out at 50℃ for 1.5h to obtain the second enzymatic hydrolysate; 4% activated carbon is added to the second enzymatic hydrolysate, and the supernatant is collected after centrifugation. The supernatant was purified, and a solution of small molecule peptides with a molecular weight of 1000~3000 Da was collected and dried to obtain sea cucumber peptides.

6. The preparation method of the traditional Chinese medicine composition according to any one of claims 1-5, characterized in that, Includes the following steps: Weigh out Cistanche deserticola, Polygonatum sibiricum, Lycium barbarum, Eucommia ulmoides, Rubus idaeus, Astragalus membranaceus, Epimedium brevicornu, Panax quinquefolius, deer antler velvet, Lonicera japonica, silkworm pupa, mulberry, Damia stolonifera, Tongkat Ali and Cabinda, pulverize them separately and then mix them to obtain mixture 1; Mixture 1 was added to water and soaked for 12 hours. Then, the mixture was heated to 55-60℃ and extracted three times. The extracts were collected three times. The extracts were concentrated to a relative density of 1.2-1.4 and dried to obtain extract 1. Extract 1, oyster peptide and sea cucumber peptide were pulverized separately and then mixed to obtain a traditional Chinese medicine extract.

7. A liver and kidney nourishing and essence and blood benefiting wine, characterized in that, The preparation method of the medicinal wine is as follows: The herbal extract obtained by the preparation method described in claim 6 is mixed with wine at a mass ratio of 1:24, soaked and stirred for 48-72 hours, and then filtered.

8. The liver and kidney nourishing and essence and blood promoting wine according to claim 7, characterized in that, The alcohol content of the wine is 38% to 65%.

9. The use of the traditional Chinese medicine composition of any one of claims 1-5 or the preparation method of claim 6 or the liver and kidney nourishing and essence and blood benefiting wine of claims 7 or 8 in the preparation of a product for nourishing liver and kidney, benefiting essence and blood.

10. Use according to claim 9, characterized in that, The product promotes the development of sexual organs, delays the weight loss trend of patients with yang deficiency syndrome, and shortens the latency period of penile erection.

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