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943results about "Hydrolysed protein ingredients" patented technology

Bone matrix compositions and methods

The present invention provides methods of improving the osteogenic and/or chondrogenic activity of a bone matrix, e.g., a dermineralized bone matrix (DBM), by exposing the bone matrix to one or more treatments or conditions. In preferred embodiments the bone matrix is derived from human bone. The treatment or condition may alter the structure of the bone matrix and/or cleave one or more specific proteins. Cleavage may generate peptides or protein fragments that have osteoinductive, osteogenic, or chondrogenic activity. Preferred treatments include collagenase and various other proteases. The invention further provides improved bone and cartilage matrix compositions that have been prepared according to the inventive methods and methods of treatment using the compositions. The invention further provides methods of preparing, testing, and using the improved bone matrix compositions. Ona assay comprises exposing relatively undifferentiated mesenchymal cells to a bone matrix composition and measuring expression of a marker characteristic of osteoblast or chondrocyte lineage(s). Increased expression of the marker relative to the level of the marker in cells that have been exposed to a control matrix (e.g., an inactivated or untreated matrix) indicates that the treatment or condition increased the osteogenic and/or chondrogenic activity of the bone matrix. Suitable cells include C2C12 cells. A suitable marker is alkaline phosphatase. The inventive methods increase the osteogenic and/or chondrogenic activity of human DBM when tested using this assay system.
Owner:WARSAW ORTHOPEDIC INC

Whey protein active peptide with antioxidant activity and preparation method thereof

The invention discloses a whey protein active peptide with antioxidant activity and a preparation method thereof. The preparation method comprises the following steps: (1) adding protease into whey protein solution for enzymolysis, wherein the enzymolysis temperature is 40-60 DEG C, and the pH value is 6.0-8.0; (2) after enzymolysis, inactivating the protease; (3) precipitating the whey protein not hydrolyzed in enzymolysis solution, and removing the whey protein to take supernatant; (4) carrying out ultrafiltration on the supernatant by an ultrafiltration membrane the cut-off molecular weight of which is less than or equal to 10,000 dalton, collecting filter liquor of peptide the molecular weight of which is less than or equal to 10,000 dalton; (5) concentrating the filter liquor; (6) desalting the concentrated liquor; and (7) concentrating the desalted solution, and then freezing and drying. In the invention, the obtained whey protein active peptide has significant antioxidant property and specific functional characteristics and stable quality, and can be widely applied to the fields of food, cosmetics, medicine and the like. The production technology is simple and is convenientfor scale production, the raw material whey protein has high use ratio, and the production cost is low.
Owner:BRIGHT DAIRY & FOOD

Method for preparing bonito stick protein hydrolysate with effect of reducing uric acid

The invention relates to a method for preparing bonito stick protein hydrolysate with an effect of reducing uric acid. The method comprises the following main steps of raw material heat treatment, restriction digestion, membrane separation-anion exchange chromatography-gel filtration chromatography separation, concentration and spray drying so as to obtain the bonito stick protein hydrolysate with an effect of reducing uric acid. The amino acid analysis indicates that the zymolyte peptide fragment primary amino acid sequence contains four amino acids, namely histidine, arginine, lysine and threonine, the total mass content of the four amino acids is 70 percent of the total amino acid content of zymolyte. MALDI-TOF-MS mass spectrum determines that the molecular weight of the main peptide effective ingredient is less than 700Da. In-vitro uric acid reduction experiments prove that the bonito stick protein hydrolysate has a remarkable effect of inhibiting generation of uric acid, and has an inhibition rate over 50 percent; an oteracil potassium molded hyperuricemic rat animal model indicates that the bonito stick protein hydrolysate can be used for remarkably reducing the level of serum uric acid and serum creatinine of rats, and shows a relatively good kidney protecting effect.
Owner:SOUTH CHINA UNIV OF TECH

Mytilus edulis enzymolysis polypeptide and preparation method and application thereof

The invention discloses a mytilus edulis enzymolysis polypeptide. The mytilus edulis enzymolysis polypeptide is characterized by containing the following amino acid sequence: Asp Leu Tyr. The mytilus edulis enzymolysis polypeptide is prepared by adopting the following steps of: (1) preparing homogenate from mytilus edulis meat, adding alkaline protease, deactivating the protease, centrifuging, and taking clear solution of the upper layer; (2) performing ultra-filtration on the clear solution, collecting hydrolysate with the molecular weight of below 3K, concentrating, and performing freeze drying; (3) performing chromatographic separation by adopting a DEAE-SepharoseFF ion exchange column; (4) performing chromatographic separation by adopting a Sephadex G-25 gel column; and (5) performing high performance liquid chromatography purification. The invention also discloses application of the mytilus edulis enzymolysis polypeptide prepared by the steps in prostatic cancer resistance. Compared with the prior art, the invention has the advantages that: the mytilus edulis is subjected to enzymolysis and purification by adopting an optimal protease and an optimal technology, a strong cell proliferation inhibiting effect is achieved when the obtained target peptide is applied to prostatic cancer resistant cells, and a feasible research path is provided for resisting prostatic cancer.
Owner:ZHEJIANG OCEAN UNIV

Polypeptide raw material prepared by enzymolysis of walnuts and application thereof

The invention relates to the technical field of biological medicines and particularly relates to a polypeptide raw material prepared by enzymolysis of walnuts and an application thereof. The polypeptide raw material is characterized in that the combination of an alkaline-dissolving and acid-precipitating technology and a biological enzymolysis technology is adopted for extracting protein in the walnuts with high efficiency and degrading polysaccharide in alkaline-dissolving matters; multienzyme synergetic hydrolysis is adopted, the content of target amino acid with the function of improving the memory, such as serine and lysine and the like, in the polypeptide raw material is enriched. The polypeptide raw material and the application have the advantages that two-stage ultrafiltration membranes are adopted for separating walnut protein hydrolysates, on one hand, the polypeptide can be separated with high efficiency, the polypeptide with the molecular weight being between 1000-10000Da is enriched, simultaneously the purpose of concentrating the target polypeptide is also achieved, and the flow of the production process is simplified; the process operation is simple, the production cost is low, no any pollution is caused, the protein recovery rate is high, and the prepared polypeptide raw material is strong in activity, has the function of improving the memory action and can be widely applied in the field of foods.
Owner:INFINITUS (CHINA) CO LTD

Preparation method of Sinonovacula constricta polypeptide with antioxidation function and application thereof

InactiveCN103740792AOptimum enzymatic hydrolysis conditionsEnzymatic hydrolysis conditions are largeHydrolysed protein ingredientsAntinoxious agentsDPPHSide effect
The invention provides a preparation method of Sinonovacula constricta enzymatic hydrolysis polypeptide, and the invention is characterized in that the method: shelling Sinonovacula constricta, cleaning and rubbing, adding water according to a mass ratio which is 1:1-4 between the material and the water for homogenate, and adjusting the pH value to 7-8; adding protease for enzymatic hydrolysis, and the addition of the protease is 0.2-0.3 m% of Sinonovacula constricta, and the enzymatic hydrolysis temperature is 60-70 DEG C, and the hydrolysis time is 2-6 hours; after enzymatic hydrolysis, killing enzyme, fetching an upper clear liquid by centrifugation, and obtaining an enzymatic hydrolysis liquid; obtaining the required Sinonovacula constricta enzymatic hydrolysis polypeptide by ultrafiltration, G-25 gel chromatographic column chromatography and reversed-phase high performance liquid chromatography elution of the enzymatic hydrolysis liquid. The invention has the advantages of simple preparation technology, and the prepared Sinonovacula constricta enzymatic hydrolysis polypeptide has the advantages of high sensitivity, good stability and little side-effect, good hydroxyl radical clearance, DPPH free radical clearance and redox performance with substantial antioxidation effect; the Sinonovacula constricta enzymatic hydrolysis polypeptide can be used for external antioxidation, and has an important meaning for further research and development of functional foodstuff and medicament based on Sinonovacula constricta enzymatic hydrolysis polypeptide, and improvement of economic value of Sinonovacula constricta.
Owner:ZHEJIANG OCEAN UNIV
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