943results about "Hydrolysed protein ingredients" patented technology

The present invention provides methods of improving the osteogenic and/or chondrogenic activity of a bone matrix, e.g., a dermineralized bone matrix (DBM), by exposing the bone matrix to one or more treatments or conditions. In preferred embodiments the bone matrix is derived from human bone. The treatment or condition may alter the structure of the bone matrix and/or cleave one or more specific proteins. Cleavage may generate peptides or protein fragments that have osteoinductive, osteogenic, or chondrogenic activity. Preferred treatments include collagenase and various other proteases. The invention further provides improved bone and cartilage matrix compositions that have been prepared according to the inventive methods and methods of treatment using the compositions. The invention further provides methods of preparing, testing, and using the improved bone matrix compositions. Ona assay comprises exposing relatively undifferentiated mesenchymal cells to a bone matrix composition and measuring expression of a marker characteristic of osteoblast or chondrocyte lineage(s). Increased expression of the marker relative to the level of the marker in cells that have been exposed to a control matrix (e.g., an inactivated or untreated matrix) indicates that the treatment or condition increased the osteogenic and/or chondrogenic activity of the bone matrix. Suitable cells include C2C12 cells. A suitable marker is alkaline phosphatase. The inventive methods increase the osteogenic and/or chondrogenic activity of human DBM when tested using this assay system.

The present invention provides a formulation for the delivery of bioactive constituents to biological surfaces, wherein said formulation comprises a suspension or solution of at least one isolated and purified casein protein or salt thereof, in water, together with at least one bioactive constituent.

Disclosed are amphiphilic compounds comprising Formula I:wherein R1, R2, and R3 are C2-C12 straight or branched alkyl; unsubstituted phenyl, biphenyl, C3-C8 cycloalkyl, or C3-C8 cycloalkenyl; or phenyl, biphenyl, C3-C8 cycloalkyl, or C3-C8 cycloalkenyl substituted with one, two, or three C1-C6 straight or branched alkyl groups; or R1 and R2 combined are C3-C8 cycloalkyl, C3-C8 cycloalkenyl; or C3-C8 cycloalkyl or C3-C8 cycloalkenyl substituted with one, two, or three C1-C6 straight or branched alkyl groups; one of R4 or R5 is selected from the group consisting of C2-C6-straight or branched alkyl-(dimethyl-N-oxide), alkyl-(dimethylamine), alkyl-(trimethylammonium), alkyl-glucosyl, alkyl-maltosyl, glucosyl, maltosyl, and polyethylene(glycosyl); the other of R4 or R5 is selected from the group consisting of H, C2-C6 straight or branched alkyl or alkenyl, C2-C6-straight or branched alkyl-(dimethyl-N-oxide); alkyl-(dimethylamine), alkyl-(trimethylammonium), alkyl-glucosyl, alkyl-maltosyl, glucosyl, maltosyl, and polyethylene(glycosyl); and salts thereof. The compounds are detergents useful in the solubilization of membrane-bound proteins.

The invention relates to a method for enzymatically obtaining protein hydrolysates for human consumption, animal feed and cosmetics. The process involves the use of a proteolytic composition derived from fish, such as Cod (Gadus morhua), to obtain hydrolysates which have a non-bitter taste and retain the flavor and aroma of the protein-containing material which is hydrolyzed: e.g. when hydrolyzing protein-containing material from marine organisms or parts thereof, such as fish, shrimp, lobster or other seafood according to the invention, a protein hydrolysate is produced that has a characteristic natural flavor of the organism Also provided are food products comprising the hydrolysates of the invention, such as soup, sauce, cheese, HVP, meat extract and flavoring agent, broth, paté, mousse, frying dough, orly dough, and pastries.

Processing methods and compositions of matter are disclosed for lipid fractions of sea cucumber intestinal mass and body wall tissue suitable for the healthfood industry and aquaculture feed pigment industry. A process and composition of matter description for obtaining a high protein de-lipidized, de-odorized meal is also disclosed.

The invention relates to cosmetic or therapeutic compositions that contain hydrolysed yeast proteins as an active ingredient, to the use of said cosmetic or therapeutic compositions, and to a method for cosmetic treatment.

The present invention describes therapeutic compositions comprising one or more minerals, including trivalent iron, divalent manganese and salts thereof, suitable in facilitating synthesis and deposition of connective tissue matrix, particularly rich of elastin and collagen, and mitogenic potential in human dermal fibroblasts. It also describes the phenomenon in which stimulation of elastogenesis by arterial SMC associates with a net decrease in proliferation of these cell types. The present invention also describes methods of treatment of human skin fibroblasts and arterial smooth muscle cells. The therapeutic compositions of the present invention comprise one or more of trivalent iron or divalent manganese or salts thereof and may be combined with an elastic tissue digest.

A method and pharmaceutical composition for ceasing milk production, for inducing involution, or for treating infection in a mammary gland of a lactating animal is described. The method is effected by direct administration of calcium chelators to the gland, or upon administration of enzymes which cause production of chelators in situ. The invention can be used to change the physiologic state of a single mammary gland of a lactating animal without significantly affecting the physiologic state of other mammary glands of the same animal. Changes resulting from use of the invention may be either transient or long lasting. The invention is expected to have uses in commercial agriculture and human medicine.

The invention relates to a food composition comprising an amino acid part having at least the amino acids leucine, isoleucine, valine, threonine and lysine in an amount of from about 35 to 90 g/100 g of the total amount of the amino acid part as a method how to produce said food composition. The invention relates to different food products comprising said food composition.

The invention provides a preparation method for a chlorella polypeptide microcapsule. The method comprises the following steps: extracting chlorella protein by using a low temperature ultrahigh pressure continuous flow cell crusher; hydrolyzing the chlorella protein with papain; filtering the hydrolyzed chlorella protein with an ultrafiltration centrifuge tube; then carrying out separation and purification by using ion exchange chromatography DEAE-52 and gel chromatography dextrangel G-25 columns; and finally utilizing complex coacervation to prepare the chlorella polypeptide microcapsule. The chlorella polypeptide microcapsule prepared in the invention has antitumor activity, e.g., the chlorella polypeptide microcapsule has inhibitory effects on in vitro growth of human hepatoma carcinoma cells HepG2 and the inhibition rate of the chlorella polypeptide microcapsule reaches 38% when concentration is 400 mu g/mL. Therefore, the chlorella polypeptide microcapsule prepared in the invention is beneficial for development and utilization of antitumor health food and medicinal products.

The present invention is directed to ophthalmic compositions containing protease-inhibiting peptide substrates. In a preferred embodiment, the protease-inhibiting peptide substrate is gelatin. The compositions may also contain a galactomannan. In a particularly preferred embodiment, the compositions contain gelatin, a galactomannan and a borate salt. The present invention also describes methods of use of these compositions to inhibit protease MMP-9, and methods of topical administration of the compositions to the eye, particularly for the treatment of dry eye.

The invention discloses a whey protein active peptide with antioxidant activity and a preparation method thereof. The preparation method comprises the following steps: (1) adding protease into whey protein solution for enzymolysis, wherein the enzymolysis temperature is 40-60 DEG C, and the pH value is 6.0-8.0; (2) after enzymolysis, inactivating the protease; (3) precipitating the whey protein not hydrolyzed in enzymolysis solution, and removing the whey protein to take supernatant; (4) carrying out ultrafiltration on the supernatant by an ultrafiltration membrane the cut-off molecular weight of which is less than or equal to 10,000 dalton, collecting filter liquor of peptide the molecular weight of which is less than or equal to 10,000 dalton; (5) concentrating the filter liquor; (6) desalting the concentrated liquor; and (7) concentrating the desalted solution, and then freezing and drying. In the invention, the obtained whey protein active peptide has significant antioxidant property and specific functional characteristics and stable quality, and can be widely applied to the fields of food, cosmetics, medicine and the like. The production technology is simple and is convenientfor scale production, the raw material whey protein has high use ratio, and the production cost is low.

The invention relates to a method for preparing a cubilose extract, which comprises the step of adding protease to a cubilose raw material to hydrolyze water-soluble cubilose protein, wherein the addition amount of the protease is 4500-40,000U per 100g water-soluble cubilose protein. The invention further relates to an application of the cubilose extract obtained by the method in preparing cosmetics or health care products.

The invention relates to a method for preparing bonito stick protein hydrolysate with an effect of reducing uric acid. The method comprises the following main steps of raw material heat treatment, restriction digestion, membrane separation-anion exchange chromatography-gel filtration chromatography separation, concentration and spray drying so as to obtain the bonito stick protein hydrolysate with an effect of reducing uric acid. The amino acid analysis indicates that the zymolyte peptide fragment primary amino acid sequence contains four amino acids, namely histidine, arginine, lysine and threonine, the total mass content of the four amino acids is 70 percent of the total amino acid content of zymolyte. MALDI-TOF-MS mass spectrum determines that the molecular weight of the main peptide effective ingredient is less than 700Da. In-vitro uric acid reduction experiments prove that the bonito stick protein hydrolysate has a remarkable effect of inhibiting generation of uric acid, and has an inhibition rate over 50 percent; an oteracil potassium molded hyperuricemic rat animal model indicates that the bonito stick protein hydrolysate can be used for remarkably reducing the level of serum uric acid and serum creatinine of rats, and shows a relatively good kidney protecting effect.

The invention discloses a mytilus edulis enzymolysis polypeptide. The mytilus edulis enzymolysis polypeptide is characterized by containing the following amino acid sequence: Asp Leu Tyr. The mytilus edulis enzymolysis polypeptide is prepared by adopting the following steps of: (1) preparing homogenate from mytilus edulis meat, adding alkaline protease, deactivating the protease, centrifuging, and taking clear solution of the upper layer; (2) performing ultra-filtration on the clear solution, collecting hydrolysate with the molecular weight of below 3K, concentrating, and performing freeze drying; (3) performing chromatographic separation by adopting a DEAE-SepharoseFF ion exchange column; (4) performing chromatographic separation by adopting a Sephadex G-25 gel column; and (5) performing high performance liquid chromatography purification. The invention also discloses application of the mytilus edulis enzymolysis polypeptide prepared by the steps in prostatic cancer resistance. Compared with the prior art, the invention has the advantages that: the mytilus edulis is subjected to enzymolysis and purification by adopting an optimal protease and an optimal technology, a strong cell proliferation inhibiting effect is achieved when the obtained target peptide is applied to prostatic cancer resistant cells, and a feasible research path is provided for resisting prostatic cancer.

The present invention is directed to compositions comprising peptides comprising a basic amino acid. e.g., arginine, and a protease.

The invention discloses a walnut blood pressure-lowering active peptide, its preparation method and application. The method includes: subjecting walnut protein to denaturation, then conducting enzymolysis by protease, and collecting walnut peptides; and subjecting the walnut peptides to molecular weight grading and debittering, thus obtaining the walnut blood pressure-lowering active peptide. The denaturation employs a microwave and ultrasonic wave combined treatment mode to make walnut protein undergo proper nature change, thus enhancing the hydrolysis degree of walnut protein, increasing the yield of walnut peptide, and improving the preparation efficiency. The debittering is carried out after molecular weight grading, so that peptide fragments with high blood pressure-lowering activity can be enriched. The walnut peptide obtained by the method disclosed in the invention has definite in vivo and in vitro blood pressure-lowering activity, and has the bitter taste concealed by beta-cyclodextrine effectively. With the advantages of easy absorption and good sensory qualities, the walnut peptide can be widely applied to functional food, health care products, and food additives.

The invention relates to a composition of invigorating the kidney and strengthening Yang. The composition is prepared from, by weight, 5-30 parts of oyster peptide, 5-30 parts of corn oligopeptide, 2-15 parts of fishbone collagen peptide, 2-20 parts of cicada pupa, 1-20 parts of cordyceps militaris, 5-25 parts of the fruit of Chinese wolfberry, 1-10 parts of ginseng, 0.1-2 parts of lycopene, 2-10parts of mulberry and 2-10 parts of cinnamon. The composition of invigorating the kidney and strengthening Yang not only has effects of tonifying Yang and reinforcing kidney but also strengthens physique and delays senescence.

The invention discloses a coilia mystus Maillard peptide with a memory improvement function as well as a preparation method and an application of the coilia mystus Maillard peptide. The method comprises following steps: coilia mystus is minced into meat paste, water is added for mixing, protease is added for enzymolysis, and an obtained supernatant is a coilia mystus enzymatic hydrolysis solution; reducing sugar is added to the enzymatic hydrolysis solution and reacts at the temperature of 90-110 DEG C for 1-2 h, a product is taken out and cooled, and coilia mystus Maillard reaction solution is obtained; the coilia mystus Maillard reaction solution is subjected to vacuum concentration and spray drying, and the coilia mystus Maillard peptide is obtained. The biological enzymolysis technology is firstly adopted to release protein peptide with the memory improvement function in coilia mystus protein to the greatest extent, and then the biological efficacy of the protein peptide is further improved through the Maillard reaction. Meanwhile, the Maillard reaction can also improve the flavor of enzymatic hydrolysates, remove fishy smell of marine products and produce pleasant fragrance.

The invention relates to a polypeptide powder of crocodile and a preparation method and application thereof, which relate to a crocodile product. The method comprises the following steps of: cleaning the crocodile meat, removing adipose tissues, cutting the crocodile meat into pieces; adding water in the crocodile meat pieces, boiling the crocodile meat pieces in a boiled water bath, homogenatingto obtain homogenated liquid of crocodile meat; adding trypsin in the obtained homogenated liquid, carrying out water bath and enzymolysis to obtain an enzymolytic mixed liquid A; placing the enzymolytic mixed liquid A in the boiled water bath, inactivating enzyme to obtain an enzymolytic mixed liquid B; cooling the obtained enzymolytic mixed liquid B to room temperature and filtering to obtain the crocodile meat enzymolytic liquid; and drying the crocodile meat enzymolytic liquid to obtain the polypeptide powder of crocodile. The polypeptide powder of crocodile has a certain function of resisting fatigue and improving the exercise ability of the body and can be applied to the preparation of medicines for resisting antifatigue and improving body exercise ability.

The invention relates to a method for preparing brain protein hydrolysate for injection and a preparation thereof. The brain protein hydrolysate is prepared from pig brain tissues passing quarantine through a specific controlled enzyme hydrolysis technique, and active ingredients of the prepared brain protein hydrolysate comprise polypeptide with a molecular weight of less than 10,000 Daltons and 16 free amino acids which are hydrolyzed amino acids; each milligram of total nitrogen of the obtained brain protein hydrolysate contains 4.68 to 7.02 milligrams of amino acids; the preparation method comprises the processes of homogenization, primary enzymolysis, inactivation, secondary enzymolysis, cation exchange column adsorption, desorption, blending, ultra-filtration, preparation and the like; when the obtained brain protein hydrolysate is used for preparing the preparation, an amino acid does not need adding; and pharmacological experiment results show that the prepared preparation has good treatment effect and good safety.

The invention relates to the technical field of biological medicines and particularly relates to a polypeptide raw material prepared by enzymolysis of walnuts and an application thereof. The polypeptide raw material is characterized in that the combination of an alkaline-dissolving and acid-precipitating technology and a biological enzymolysis technology is adopted for extracting protein in the walnuts with high efficiency and degrading polysaccharide in alkaline-dissolving matters; multienzyme synergetic hydrolysis is adopted, the content of target amino acid with the function of improving the memory, such as serine and lysine and the like, in the polypeptide raw material is enriched. The polypeptide raw material and the application have the advantages that two-stage ultrafiltration membranes are adopted for separating walnut protein hydrolysates, on one hand, the polypeptide can be separated with high efficiency, the polypeptide with the molecular weight being between 1000-10000Da is enriched, simultaneously the purpose of concentrating the target polypeptide is also achieved, and the flow of the production process is simplified; the process operation is simple, the production cost is low, no any pollution is caused, the protein recovery rate is high, and the prepared polypeptide raw material is strong in activity, has the function of improving the memory action and can be widely applied in the field of foods.

The invention discloses a method for preparing walnut polypeptide nano-selenium with anti-tumor activity. The method comprises the following steps: (1) performing limited enzymatic hydrolysis on walnut dreg; (2) concentrating, and spray-drying to obtain walnut polypeptide; (3) adding an inorganic selenium source and vitamin C into the walnut polypeptide solution to prepare functional nano-selenium sol; (4) dialyzing the sol; and (5) carrying out freeze drying to obtain the walnut polypeptide nano-selenium. The dissolved walnut polypeptide nano-selenium is orange sol, and the morphology of the polypeptide nano-selenium is observed by a scanning electron microscope, and nano-particles with uniform particle size can be observed. The walnut polypeptide nano-selenium disclosed by the invention can be used for preparing selenium supplement agent healthy products and medicines. Cell experiments prove that the sol has a remarkable anti-tumor bioactivity, and is expected to become a high-efficiency low-toxicity preparation for treating cancers, in particular breast cancer.

The invention provides a preparation method of a small water turtle anti-tumor polypeptide. The method comprises the following steps: mixing 3 to 20g of meat paste of small water turtle and ultrapure water; agitating to obtain a mixed solution at concentration of 10 to 50% (w/v); adding trypsin to perform enzymolysis; deactivating enzyme after enzymolysis; cooling until reaching room temperature; centrifuging enzymatic hydrolysate; collecting supernate; sequentially filtering through ultrafiltration membranes which respectively have molecular cut off of 10KD, 5KD and 3KD, so as to obtain three enzymatic hydrolysates; performing sephadex G-15 column chromatography for 0-3K enzymatic hydrolysate; eluting with water; collecting four polypeptide components under a certain detection wavelength so as to obtain small water turtle anti-tumor polypeptide. The prepared small water turtle anti-tumor polypeptide is beneficial for the development and utilization of anti-tumor drugs and health foods.

The present invention is antioxidant peptide mixture obtained through hydrolyzing collagen and its preparation process. The preparation process includes hydrolyzing collagen with protease to obtain coarse product of antioxidant peptide mixture; precipitating or filtering to obtain hydrolysate with fat, macro molecular protein, polypeptide and impurity eliminated; and final separating purification and concentration to obtain the antioxidant peptide mixture. The present invention has wide material source and low cost, and the product has powerful antioxidant effect, and may be used as food additive as well as in cosmetics, health product, feed, etc.

The invention provides a preparation method of Sinonovacula constricta enzymatic hydrolysis polypeptide, and the invention is characterized in that the method: shelling Sinonovacula constricta, cleaning and rubbing, adding water according to a mass ratio which is 1:1-4 between the material and the water for homogenate, and adjusting the pH value to 7-8; adding protease for enzymatic hydrolysis, and the addition of the protease is 0.2-0.3 m% of Sinonovacula constricta, and the enzymatic hydrolysis temperature is 60-70 DEG C, and the hydrolysis time is 2-6 hours; after enzymatic hydrolysis, killing enzyme, fetching an upper clear liquid by centrifugation, and obtaining an enzymatic hydrolysis liquid; obtaining the required Sinonovacula constricta enzymatic hydrolysis polypeptide by ultrafiltration, G-25 gel chromatographic column chromatography and reversed-phase high performance liquid chromatography elution of the enzymatic hydrolysis liquid. The invention has the advantages of simple preparation technology, and the prepared Sinonovacula constricta enzymatic hydrolysis polypeptide has the advantages of high sensitivity, good stability and little side-effect, good hydroxyl radical clearance, DPPH free radical clearance and redox performance with substantial antioxidation effect; the Sinonovacula constricta enzymatic hydrolysis polypeptide can be used for external antioxidation, and has an important meaning for further research and development of functional foodstuff and medicament based on Sinonovacula constricta enzymatic hydrolysis polypeptide, and improvement of economic value of Sinonovacula constricta.

The invention relates to a active bone peptide for treating and preventing osteoporosis and process for its preparation, which comprises the following steps: (1) cleaning bones of animal limbs, sterilizing, disintegrating, charging distilled water, controlling hydrolysis with proteinase, filtering the supernatant fluid, (2) degreasing the supernatant fluid, concentrating, ethanol extracting, filtering the supernatant fluid, (3) dealcoholizing the supernatant fluid, centrifugal filtrating, gathering the filtering medium, (4) making the active bone peptide into any one of the pharmacological dosage forms.

The invention relates to a method for preparing a prostate cancer-resisting mussel enzymolytic extract. The method comprises the following steps: 1) preparing a sample by cleaning mussel, removing the shell thereof, taking out the flesh and homogenating, wherein the ratio of solid to liquid is 1:2 to 1:3, the pH value is adjusted to 9.5 to 10 and the temperature is kept between 25 and 30 DEG C for 20 to 30 min; and 2) adding Alcalase, wherein the enzyme adding quantity is 1500 to 2000u/g, the enzymolysis temperature is 45 to 50 DEG C and the enzymolysis time is 6 to 8 h; and carrying out inactivation and centrifugation and taking the liquid supernatant so as to acquire the enzymolytic product. The invention also discloses an application of the enzymolytic extract in resisting the prostate cancer. Compared with the prior art, the invention has the advantages that a better technique is acquired by controlling the enzyme selection, the ratio of solid to liquid, the pH value, the adding quantity of enzyme, the temperature and the enzymolysis time, and the product has a good function of inhibiting the multiplication of prostate cancer cells.

The invention relates to the technical field of hygiene products, particularly relates to nursing and health care of female privates, and provides a female privates nursing composition and a preparation method and application thereof. The composition comprises, by weight, 12.5-26.5 parts of wetting agents, 15-25 parts of extract of radix sophorae flavescentis, 10-20 parts of extract of cortex phellodendri, 10-15 parts of extract of fructus cnidii, 10-15 parts of extract of herba leonuri, 5 parts of extract of rhizoma curcumae, 5-10 parts of extract of stigma croci;, 1-5 parts of hydrolyzed collagen, 3-5 parts of ethyl alcohol, 0.1-2 part of chlorhexidine acetate, 0.3-1 part of poloxamer, and 0.1-1 part of borneol. Various components of the female privates nursing composition have remarkable synergistic effect and can effectively clean the privates, relieve uncomfortable feelings of the privates, suppress bacterium breeding, repair virginal membrane, relieve itching and deodorize, and even assists in beautifying and compacting after long-term use.

A periodontal disease preventive and ameliorative agent with milk-derived basic protein as its effective ingredient, which protein is obtained by contacting a milk or milk-derived ingredient with cation-exchange resin, and then eluting a fraction adsorbed by the resin using an elution solution and which protein has an isoelectric point within a range of 7.5~11, and food/drink and a medicament such as toothpaste and gargling agents, which contain this periodontal disease preventive and ameliorative agent.