Method for preparing pharmaceutics of hydrolysate of brain protein

Abstract

A hydrate of brain protein is prepared from pig's brain through adding purified water, homogenizing, heating, cooling, regulating pH=1.5-2.0, enzymolyzing, regulating pH=7.7-8.0, enzymolyzing, regulating pH=2.5-3.0 freezing, thawing, filter, regulating pH to become neutral, ultrafiltering, concentrating, sterilizing, adding amino acids, regulating peptide map, diluting, and steam sterilizing.

Description

technical field [0001] The invention relates to a preparation method of medicine, in particular to a preparation method of cerebroprotein hydrolyzate preparation. Background technique [0002] Cerebroprotein hydrolyzate, as an active brain polypeptide substance extracted from healthy and fresh animal brain tissue by multi-enzyme hydrolysis, contains a variety of essential amino acids for human brain and important elements such as cephalin, lecithin, and peptide nerve growth factor. It acts on the central nervous system in various ways, regulates and improves the metabolism of neurons, promotes the formation of synapses, induces the differentiation of neurons, and further protects nerve cells from various ischemia and neurotoxin damage. It can pass through the blood-brain barrier, promote protein synthesis in the brain, affect the respiratory chain, protect against hypoxia, and improve energy metabolism in the brain. Activates adenylyl cyclase to catalyze other hormone syste...

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Problems solved by technology

Its shortcoming is: (1), equipment investment is big, production cycle is long, and production efficiency is low; Use chromatographic method to purify active ingredient, each process needs 30~48 hours, and production cycle is long; , requires Sephadex G-25 gel column chromatography to be carried out in the 2-8°C cold storage workshop, and the Sephadex G-25 gel chromatography material must be imported, which is expensive; annual production of 1 million (10ml) cerebroprotein hydrolyzate injections Calculated by liquid, the above-mentioned investment is more than 2 million yuan; the steps of vacuum concentration and centrifugation are used, the production efficiency is low, and it is not conducive to the control of drug quality

(2), use a large amount of organic chemical solvents, acetone and ether have extremely low boiling points, are flammable, explosive, poisonous dangerous chemicals, ether is also used as an anesthetic, use a large amount of acetone and ether to precipitate brain protein first It is made into brain powder. This step is very dangerous and must be produced in an explosion-proof workshop. The operation is cumbersome, pollutes the environment, and endangers the health of production operators.

Its shortcoming is: (1) use ultrafiltration membrane to carry out ultrafiltration concentration recovery rate is low: although this process has adopted ultrafiltration technology, what use is that the ultrafiltration membrane that cut-off molecular weight is 1000 is concentrated, and effective active ingredient brain polypeptide is different The degree of loss, that is, loss in the permeate, greatly reduces the yield

(2) Multiple passes through microporous membranes with a pore size of 0.5-1um and ultrafiltration membranes with a cut-off molecular weight of 70,000 and 10,000 to obtain ultrafiltrate, which makes the operation more cumbersome

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