The invention relates to preparation and application of a
copper-
molybdenum separation inhibitor. The preparation method of the inhibitor comprises the following steps that under a catalytic stirringcondition, the reaction of
mercaptoacetic acid with monoethanolamine is carried out at the temperature of 90-100 DEG C, the reaction time is 4-5 hours, and the
copper-
molybdenum separation inhibitor,specially, N-(2-hydroxyethyl)-2-mercaptoacetamide which is subjected to
dehydration condensation kneading is obtained; the application of the prepared
copper-
molybdenum separation inhibitor is characterized in that the copper-molybdenum separation inhibitor is applied to flotation separation of copper-molybdenum mixed concentrate, the N-(2-hydroxyethyl)-2-mercaptoacetamide is prepared, and strongparent-mine polar group (-SH) and hydrophilic polar group (-OH and -CONH) are provided, so that the
copper ore can be effectively inhibited, and the copper and molybdenum separation is achieved; the inhibitor is simple in preparation method, remarkable in inhibition effect, less in use amount, free of pungent smell, stable in property, safety in addition and convenient to use; and the N-(2-hydroxyethyl)-2-mercaptoacetamide is matched with
sodium sulfide to use so that the synergistic effect can be brought into full play, the copper-molybdenum separation precision is improved, and the method isparticularly suitable for flotation separation application of
molybdenite and
copper sulfide ore in the copper-molybdenum mixed concentrate.