The present application relates to the field of
medicine manufacturing, and relates to a preparation method of
natural source cholesterols for injection, which comprises the following steps: taking
fish oil, cooling and winterizing, taking the lower
sediment, heating to dissolve it into clear
winterization fraction, adding the
winterization fraction into a
chromatographic column filled with mixed filler of adsorbent and filter aid which are mixed uniformly in a physical way to perform circulation
chromatography, emptying the
winterization fraction after the circulation ends, adding eluent to the
chromatographic column, and recovering the
solvent after the eluent is combined to obtain cholesterols concentrate. The cholesterols concentrate is added with first
crystallization solvent to recrystallize and refine twice, the cholesterols crude product after recrystallization is taken and added with second
crystallization solvent to recrystallize and refine, and the product is dried under reduced pressure to obtain cholesterols for injection. The present application solves the technical problem of preparing cholesterols for injection from
fish oil, is suitable for large-scale industrialization of cholesterols, can be applied to preparation of complex preparations such as liposomes, and improves the safety of
drug use.