Polyunsaturated fatty acid-containing oil product and uses and production thereof

A technology for oil products and fats, applied in the field of oil products containing polyunsaturated fatty acids and their use and preparation, can solve the problems of increased time and cost

Inactive Publication Date: 2008-08-27
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These processes add time and cost to product preparation and may introduce chemicals in the refining process that are not acceptable for natural or organic product markets

Method used

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  • Polyunsaturated fatty acid-containing oil product and uses and production thereof
  • Polyunsaturated fatty acid-containing oil product and uses and production thereof
  • Polyunsaturated fatty acid-containing oil product and uses and production thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] Embodiment 1: Preparation of high quality crude oil

[0134] In a fermenter, a microorganism of the genus Schizochytrium enriched in DHA oil is grown to obtain a fermented liquid. The fermentation broth was collected and contacted with Alcalase(R) 2.4, an enzyme that lyses Schizochytrium cells. The resulting lysed cell mixture was an emulsion which was contacted with a 27% aqueous solution of isopropanol. The mixture was mixed by stirring and then centrifuged to obtain a substantially non-emulsified two-phase product. The heavy phase contained components of spent fermentation broth and the light phase contained DHA-enriched oil with some isopropanol and water. Drying the light phase yields a high quality crude oil.

Embodiment 2

[0135] Example 2: Minimal treatment of algae oil

[0136] This example illustrates the preparation of an oil with minimal processing in accordance with the present invention.

[0137] Oils with minimal handling are produced on a large scale. Two hundred kilograms of high quality crude oil (prepared as described in Example 1), prepared by microorganisms of the genus Schizochytrium, was heated to 65°C to 70°C under nitrogen, Contains DHA. Then, about 0.2% (the ratio of its weight to the weight of the oil) of a 50% citric acid solution was added to the oil and mixed under nitrogen for 30 to 45 minutes. Next, 0.2 to 0.5% (the ratio of its weight to the weight of the oil) of filter aid is added to the oil and filtered to remove any impurities present in the oil. The oil was then deodorized at 210°C at a feed rate of 180 kg / hour. The deodorized oil is then supplemented with tocopherol, ascorbyl palmitate and rosemary extract. In Table 1, the characteristics of the oil for each ...

Embodiment 3a

[0140] Example 3a: physical refining

[0141] This example illustrates the preparation of an oil with minimal processing in accordance with the present invention.

[0142] Get about 600kg high-quality crude oil (prepared as described in Example 1) (FFA24"Hg). Then, add diatomaceous earth (0.1-0.5% w / w, typically 0.2%), the oil is filtered through a Sparkler filter. Then, the oil is deodorized at 210-225°C at a flow rate of 180-225kg / hr. After deodorization, add Antioxidant. This process results in an oil that is semi-solid at room temperature.

[0143] The oil yield from this treatment was -92-97%. Table 2 shows the mass data for these steps in the presence of antioxidants.

[0144] Table 2

[0145]

[0146] The FFAs of the deodorized oils were measured before and after addition of antioxidants. A significant increase (approximately 2-fold) in FFAs was observed after the addition of antioxidants.

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Abstract

The present invention includes a solid fat composition that includes an oil having saturated fat and a microbial oil having a long chain polyunsaturated fatty acid and an emulsifier. In particular, the solid fat composition can have high levels of long chain polyunsaturated fatty acid and low amounts of emulsifiers. In preferred embodiments, the polyunsaturated oil is an unwinterized microbial oil. The invention also relates to methods for making such compositions and food, nutritional, and pharmaceutical products comprising said compositions. The present invention also includes a microbial oil product prepared by extracting an oil-containing fraction comprising at least one LC-PUFA from a microbial biomass, and treating the fraction by a process of vacuum evaporation, wherein the oil product has not been subject to one or more of a solvent winterization step, a caustic refining process, a chill filtration process, or a bleaching process.

Description

technical field [0001] The present invention relates to oil products comprising polyunsaturated fatty acids and their use, for example in solid fat compositions comprising long chain polyunsaturated fatty acids of microbial origin and thickeners. The invention also relates to methods of preparing these products and methods of food, nutraceuticals and pharmaceuticals comprising said compositions. Background technique [0002] It is desirable to increase dietary intake of a variety of beneficial nutrients. Particularly beneficial nutrients include fatty acids, such as omega-3 and omega-6 long chain polyunsaturated fatty acids (LC-PUFA). Omega-3 PUFAs are considered important dietary compounds for preventing atherosclerosis and coronary heart disease, relieving inflammatory states, and delaying tumor cell growth. ω-6PUFAs not only serve as structural lipids in the human body, but also serve as precursors of various inflammatory factors such as prostaglandins and leukotrienes....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23D7/00
Inventor 贾奥德·菲希塔利尼尔·F·莱宁格杰苏斯·R·阿布里尔纳泽·阿梅德S·P·贾纳卡·N·塞纳纳亚科
Owner DSM IP ASSETS BV
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