The invention discloses a method for extracting and analyzing
palmitic acid in pepper
essential oil, and belongs to the technical field of food detection.The method comprises the steps that S1, processed black pepper particles and processed white pepper particles are obtained, the prepared black pepper particles and the prepared white pepper particles are smashed respectively, and black pepper
powder and white pepper
powder are obtained; s2, water and steam are added into the black pepper
powder and the white pepper powder for
distillation, after
distillation is completed, an
essential oil layer is taken, and white pepper
essential oil and black pepper essential oil are obtained; and S3, respectively adding a
petroleum ether extracting solution into the essential oil for extraction, adding a
potassium hydroxide and
methanol mixed solution for methyl esterification, adding
ultrapure water, centrifuging, taking supernatant, filtering, diluting the filtered sample with chromatographic-grade normal
hexane, and detecting the content of
palmitic acid by using a gas chromatograph-
mass spectrometer. According to the method disclosed by the embodiment of the invention, the composition of special effective substances of the pepper essential oil is determined by researching a pepper essential oil
palmitic acid component detection technical method, and an important basic
technical support is provided for research, development and application of pepper high-quality products in China.