Method for regulating and controlling microbial enhanced oil recovery

A microbial oil recovery and microbial technology, applied in microbial-based methods, methods using microorganisms, microorganisms, etc., can solve problems such as difficulty in achieving oil recovery and inability to obtain microbial knowledge of oil reservoirs, and achieve full performance and utilization. High, improve the effect of oil displacement performance

Active Publication Date: 2010-04-28
DAQING HUALI ENERGY BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing technology uses the culture method to analyze the microbial community structure in the oil reservoir, and the results obtained only reflect a small part of the microbial community in the oil reservoir, and it is impossible to obtain a comprehensive and systematic understanding of the microbial community and functions in the oil reservoir. Based on this understanding , changing the community composition in the system by injecting a nutrient system or a small number of bacterial species to play the role of oil displacement functional bacteria, which often has a large blindness and randomness, so it is difficult to achieve the purpose of stably increasing the recovery rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Regulatory effects of different nutrient systems and temperatures on microbial growth and metabolism

[0023] System composition: an oilfield formation water + crude oil or sucrose + oil displacement bacteria + baseline bacteria (microorganisms in formation water and microorganisms in activated sludge) + nutrient system. Among them, oil displacement bacteria include hydrocarbon degrading bacteria TF2 and biosurfactant producing bacteria HN1. TF2 can grow with glucose and n-hexadecane as carbon sources. When cultured with mixed hydrocarbons from n-hexadecane to n-docosane as carbon source, TF2 preferentially utilizes n-hexadecane. TF2 grows well under the conditions of temperature 50°C-65°C, pH=6-9, and salinity 2 HPO 4 (0.08%), NaH 2 PO 4 (0.04%), MgSO 4 ·7H 2 O (0.02%), CaCl 2 2H 2 O (0.01%), NaCl (0.02%). They were cultured at 37°C, 55°C, and 65°C, and transferred 4 times to investigate the regulation of different carbon and nitrogen sources on microbial m...

Embodiment 2

[0068] A method for controlling microbial oil recovery, specifically comprising the steps of:

[0069] (1) Using 16S rDNA library, PCR-DGGE, RT-PCR and other molecular biology methods to analyze the microbial community structure in oil reservoir production fluid:

[0070] The water samples produced in the test oil reservoir were collected, and the genomic DNA of the microbial community was extracted using the method disclosed in "Molecular Analysis of the Diversity of the Microbial Community in the Oil Reservoir" (Ph.D. A genome library was constructed, and the RFLP method was used to analyze the diversity of the microbial community in the oil reservoir, thereby obtaining the community structure of the microbial community in the oil reservoir environment. Using the method disclosed in "Detecting the Abundance of Environmental Pseudomonas Bacteria by Real-time Fluorescent Quantitative PCR" (Zhao Chuanpeng et al., Journal of Southeast University, Volume 36, No. 1, 2006), the abu...

Embodiment 3

[0079] A method for regulating oil recovery in a nutrient system corresponding to microorganisms, the method comprising the steps of:

[0080] (1) Detection of metabolites in the output fluid:

[0081] According to the method disclosed in the literature "Research on the Optimum Conditions for Preparation of Rhamnose by Microbial Fermentation" (Li Zuyi, etc., "Journal of Biological Engineering", 1999, No. 1), the content of glycolipids in the oil well production fluid was analyzed, and the literature "In the Microbial Fermentation Liquid "Measurement of lipopeptide biosurfactants" (Chen Tao et al., "Oilfield Chemistry", 2004, 04th issue) discloses the method to analyze lipopeptide content, thus obtains the information of functional microbial metabolites in the oil reservoir. According to the change analysis of metabolites, information such as the abundance and activity of microorganisms that produce the metabolites can be obtained.

[0082] (2) Adjust the composition of the nu...

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Abstract

The invention relates to a method for regulating and controlling microbial enhanced oil recovery. The method comprises the following steps: (1) analyzing a microbial community structure in oil deposit produced fluid and / or metabolic products in detection produced fluid by adopting a molecular biological method; (2) regulating a microbe to be injected into the oil deposit and / or a nutritious system corresponding to the microbe; (3) injecting the regulated microbe and / or the nutritious system corresponding to the microbe into the oil deposit by a water injection well; and (4) gathering crude oil by a corresponding beneficial producing well. Compared with the prior art, the method for regulating the microbial community structure in the oil deposit contributes to the direction evolution of oil recovery, can sufficiently play the performance of functional microbes, injects the nutritious system specifically, and avoids blindness in application of the nutritious system, thus the method is a scientific, economic and effective microbial oil recovery method.

Description

technical field [0001] The invention relates to crude oil exploration and exploitation technology, in particular to a method for regulating and controlling microbial oil recovery. Background technique [0002] Due to the complex geological conditions of continental reservoirs in my country, nearly two-thirds of the crude oil remains underground after water flooding, the recovery rate of crude oil in oilfields is generally low, and the difficulty of replacing reserves and other severe situations, there is an urgent need to research and develop efficient, adaptable To meet the society's demand for energy with highly innovative enhanced oil recovery technology. [0003] Studies have shown that microbial oil flooding is a technology with a wide range of adaptability and potential for enhancing oil recovery, and has broad application prospects. This technology enhances oil recovery through the beneficial activities of microorganisms (degradation of crude oil, etc.) and metabolite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E21B43/22C12S1/00C09K8/582C12R1/40C12R1/39C12R1/385C12R1/01C12R1/07C12R1/145C12R1/125
CPCC12N1/20C09K8/582C12P39/00C12N1/36
Inventor 牟伯中刘金峰杨世忠刚洪泽
Owner DAQING HUALI ENERGY BIOLOGICAL TECH
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