110 results about "Microbial enhanced oil recovery" patented technology

An apparatus and method produces nitrate ions on-site from water, natural gas and air extracted in proximity to the apparatus. The apparatus generates nitrate ions and brings the nitrate ions into contact with an aqueous system. Hydrogen sulfide present in the aqueous system is removed and the production of hydrogen sulfide by sulfate-reducing bacteria (SRB) is eliminated by introducing into the system nitrate ions, whereby denitrifying microorganisms, using nitrate, outcompete the sulfate-reducing bacteria for the available carbon nutrients, thus preventing the SRB from producing hydrogen sulfide. Nitrate ions generated by the apparatus and added to the aqueous system which contains the denitrifying microorganisms can enhance oil recovery by means of microbial enhanced oil recovery mechanisms.

The present invention is related to the field of microbial enhanced oil recovery (MEOR). In particular, the invention concerns new, efficient, economical and environmentally safe microbial methods to enhance oil recovery, as well as microorganisms useful in such methods.

A method of microbial enhanced oil recovery for recovering oil from an oil-bearing rock formation is provided. The methodology uses a consortium of bacteria including a mixture of surfactant producing bacteria and non-surfactant enzyme producing bacteria which may release hydrocarbons from bitumen containing sands. The described bioprocess can work with existing petroleum recovery protocols. The consortium microorganisms are also useful for treatment of above oil sands, ground waste tailings, subsurface oil recovery, and similar materials to enhance remediation and / or recovery of additional hydrocarbons from the materials.

A method of microbial enhanced oil recovery from an oil-bearing formation that involves treating the water that is to be injected into the oil-bearing formation to enable microbial activity and adding oxygen to aid microbial activity. The treatment applied to the water is based, at least in part, upon establishing at least one condition in the oil-bearing formation favorable to microbial activity that enhances movement of oil from the oil-bearing formation.

Controlling microbial growth and activity during Microbial Enhanced Oil Recovery processes is disclosed. Specific control of microbial growth and activity in this process results in prevention of nutrient loss in transit and allows better targeting of microbial activity to the desired subsurface location(s).

The invention relates to a microbial enhanced oil recovery technique, in particular to a method which is suitable for constructing an oil reservoir oil displacement microbial community to improve crude oil recovery ratio in the middle and later periods of a water-drive reservoir. The technical scheme is that the method for constructing the oil reservoir oil displacement microbial community comprises but is not limited to the following steps of: a, performing field sampling analysis on a target oil reservoir; b, determining a preliminary construction scheme according to field sampling test results; and c, optimizing the construction scheme by using a mixed culture experiment. The method has the following advantages that: (1) aiming at a specific oil reservoir, a targeted assessment is performed on oil reservoir microbial ecology to find out missed parts of a metabolic link, and the oil reservoir has high adaptability; and (2) the operability is strong. The method is used for adjusting the microbial community to evolve towards a target structure in a targeted mode, thereby improving the pertinence and the effectiveness of the field implementation of the oil recovery ratio by using microbes.

The invention discloses a method for adjusting and controlling microbial enhanced oil recovery, which comprises the following steps: (1) analyzing the microbial community structure in produced fluid of an oil reservoir via molecular biological method and / or detecting the metabolites in the produced fluid; (2) adjusting the microorganism(s) to be injected into the oil reservoir and / or the nutrient system corresponding to the microorganism(s); (3) injecting the adjusted microorganism(s) and / or the nutrient system corresponding to the microorganism(s) into the oil reservoir through a water injection well; and (4) obtaining the crude oil from a corresponding beneficial oil producing well. Compared with the prior art, the method of the present invention adjusts microbial community structure in the oil reservoir to evolve toward the direction of facilitating oil production, and the performance of the functional microorganism(s) can be completely realized; the nutrient system is pertinently injected to avoid the blindness of using the nutrient system. Therefore, the method is scientific, economical and effective for the microbial enhanced oil recovery.

The present invention provides methods for increasing the viscosity of the drive fluid for displacing oil from a subterranean formation by the use of microorganisms selected or modified for the ability to produce cell free polymers without the formation of any significant bioplugging biofilm or capsule.

The invention discloses a method for improving the recovery ratio of indigenous microbial enhanced oil recovery. The method comprises the following steps: acquiring a fluid sample on a target reservoir site; analyzing an indigenous microbial community structure; determining an indigenous activator and the injection quantity thereof; selecting an on-site injection process of the indigenous activator; evaluating a physical analog enhanced oil recovery; performing on-site tests, and tracking and analyzing of on-site test effects. The method is reasonable, simple in process, easy to operate, safe and reliable, beneficial to on-site popularization and application, and low in investment and cost, greatly improves the on-site test effect, improves the recovery ratio of more than 12% during indigenous microbial enhanced oil recovery on-site tests, and thus can be widely applied to on-site tests for improving the recovery ratio of indigenous microbial enhanced oil recovery.

The invention belongs to the technical field of microbial enhanced oil recovery, and particularly relates to a method for selectively activating water-drive oil reservoir indigenous microorganisms to produce object surface active agents so as to improve the crude oil recovery efficiency. The method includes the following steps of firstly, conducting sampling on site, analyzing the microorganisms which are contained in an oil reservoir sample and produce the object surface active agents, and analyzing the oil-water physicochemical property; secondly, determining dominant population capable of producing the object surface active agents and key metabolic substrates of the dominant population, and designing an active agent formula system; thirdly, optimizing an active agent formula; fourthly, optimizing the active agent on-site injection technology; fifthly, conducting a test on site. The method has the advantages of being high in pertinence and reliability, wide in oil reservoir application range, simple in method, high in operability, good in on-site implementing effect and the like, and therefore the method can be widely applied to microorganism oil displacement on-site tests.

The invention provides a microbacterium oxydans for degrading a polycyclic aromatic hydrocarbon and the application thereof. Particularly, the invention provides a microbacterium oxydans Ji2 strain with a preservation number of CGMCC No.9072 and the application effect of the microbacterium oxydans in emulsifying and degrading crude oil and the polycyclic aromatic hydrocarbon and performing indoor physical simulation oil displacement. According to the microbacterium oxydans strain, the crude oil can be emulsified and degraded under aerobic and anaerobic conditions by taking the crude oil or the polycyclic aromatic hydrocarbon as the only carbon source and energy source, the degradation rate of the degraded polycyclic aromatic hydrocarbon, such as naphthalene is up to 100 percent, and analysis on the degraded crude oil shows that aromatic hydrocarbon and colloid, which are the components of the crude oil, are mainly degraded. The stain can be used for degrading the polycyclic aromatic hydrocarbon in an environment by the performance of the stain, and can also be applied to microbial enhanced oil recovery, and the oil recovery is improved.

The invention discloses a microbial enhanced oil recovery method and belongs to the technical field of tertiary oil recovery. The method is characterized by comprising the following steps: 1, conducting displacement of reservoir oil through biosurfactant; 2, adjusting injection-production patterns; 3, conducting displacement of reservoir oil through indigenous microorganisms; 4, conducting oil recovery through microorganism single well huff-and-puff. According to the method, firstly, the biosurfactant is injected, so that the displacement efficiency of a water-driven oil reservoir is improved; then through adjustment of the injection-production patterns, the swept volume, in the oil reservoir, of the indigenous microorganisms is expanded; finally, through the microorganism single well huff-and-puff treatment, crude oil in the immediate vicinity of an oil well is displaced out, and thereby the purpose of further raising the recovery ratio of the oil reservoir is achieved finally. The method has the advantages that activator has the advantages of being wide in source, low in price and harmless to strata; the method is wide in oil reservoir application range and particularly suitable for an oil reservoir which is exploited in a water-driven mode and is medium and high in permeability; the process is simple, and the pertinence and operability are great; the field test effect is good, and the enhanced oil recovery rate is testified by field tests to be larger than 15.0%. Therefore, the method can be widely applied to the technical field of microbial enhanced oil recovery.

A post cold heavy oil production with sand (“CHOPS”) microbial enhanced oil recovery method comprises selecting a well having at least one wormhole and that has completed primary CHOPS production. When the reservoir does not contain enough of a gas producing indigenous microbe, an injectant is prepared comprising a gas-producing microbe, a nutrient suitable for the microbe, and a fluid base. The injectant is injected through the well and into the at least one wormhole in the reservoir; the well is shut in until pressure in the well reaches the target pressure; and then the well is produced.

The invention relates to a method for quantifying indigenous microorganisms for producing lipopeptide surfactant in microbial flooding reservoir. Primers are designed according to lipopeptide synthetase genes of microorganisms for producing lipopeptide surfactant, and fluorescent quantitative PCR (polymerase chain reaction) is performed. In optimized reaction procedures and reaction system, the lipopeptide synthetase genes obtained from a standard lipopeptide-producing strain is connected with a carrier to construct a recombinant plasmid used as a standard sample for real-time quantitative PCR; the standard sample is subjected to 10-time gradient dilution and used as a template for fluorescent quantitative PCR, and a standard curve is drawn; a microbial flooding reservoir sample is subjected to simple treatment and used as a template for fluorescent quantitative PCR; and whether data of replicate samples are identical and whether initial templates having different numbers of copies have the same amplification efficiency are judged according to linear relationship R2 and amplification efficiency E. When being used for detecting microorganisms for producing lipopeptide surfactant in reservoir under optimized reaction conditions, the lipopeptide synthetase specific primers have higher stability and sensitivity, thereby being widely used in the field of microbial enhanced oil recovery.

Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

The invention relates to a method for extracting the total DNA of a microorganism sample during the microbial oil recovery process, which comprises the steps of selecting a proper PCR-DGGE primer for extracting the total DNA of a microorganism sample by adopting the method for monitoring an advantageous flora in microbial enhanced oil recovery, wherein the sequence of the PCR-DGGE primer is DQF338: 5'-CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGGACTCCTACGGGAGGCAGCAG-3' DQR534:5'-ATTACCGCGGCTGCTGG-3'. By adopting the above primer, the actual amount of microorganisms in an oil deposit can be accurately reflected. Meanwhile, the distribution, migration and change of the oil recovery microorganisms in the oil deposit can be accurately and quickly analyzed. Thus, the method provides an important theatrical instruction for improving oil recovery ratio.

The present invention discloses a composite microbial agent, a preparation method therefor and an application thereof, belonging to the field of microbial enhanced oil recovery. The present invention firstly discloses a composite microbial agent, comprising: an Anoxybacillus sp. microbial agent, a Ureibacillus sp. microbial agent, and a biosurfactant. The present invention further discloses a composite microbial agent preparation method, comprising: mixing the Anoxybacillus sp. microbial agent, the Ureibacillus sp. microbial agent and the biosurfactant. The composite microbial agent of the present invention can be applied to improve the recovery rate of high pour point oil, improve the physical properties of high pour point oil, decrease viscosity of high pour point oil, increase fluidity of high pour point oil, and eliminate blocking wax at the near wellbore area of the well shaft of high pour point oil, thereby dramatically increasing the yield of high pour point oil.

The invention provides a novel thermophilic and salt-resistant strain capable of degrading raw oil and generating an emulsifying agent and an application thereof. Particularly, the invention relates to anoxybacillus rupiensis with the collection number CGMCC No.6614 and an anaerobic bacillus preparation containing the anoxybacillus rupiensis. The invention also relates to an application of the anoxybacillus rupiensis or the preparation thereof in fermenting, culturing and producing biological surface active substances, an application in degrading the raw oil and an application in displacing oil. The anoxybacillus rupiensis strain can grow under the high-temperature and high-salt environment and can adopt the raw oil and molasses as a carbon source to generate the macromolecular emulsifying agent under the aerobiotic and anaerobic conditions; and the biological emulsifying agent has strong emulsifying capability, and also has the effects of effectively improving the solubility of alkane or the raw oil in water and obviously promoting the degradation of the strain to the raw oil. The strain and the emulsifying agent generated thereby can be used for microbial enhanced oil recovery and can increase the oil recovery rate.

The invention provides acinetobacter junii for producing a bio-surfactant and application of the acinetobacter junii. The preservation number of the acinetobacter junii is CGMCC No. 13206. The acinetobacter junii has good surface activity producing performance, can produce multiple types of rhamnolipid surfactants through metabolism, can be applied to microbial enhanced oil recovery, has the characteristics of being high in oil reservoir environment adaptability, good in biological stability and the like, and can effectively increasing the enhance oil recovery.

The invention belongs to the technical field of microbial enhanced oil recovery and particularly relates to a low-permeability reservoir microbial enhanced oil recovery composite preparation and an application method thereof. The low-permeability reservoir microbial enhanced oil recovery composite preparation is mixed and prepared by pseudomonas aeruginosa CGMCC1.10452 and the fermentation culture of bacillus subtilis CGMCC1.2172 and CGMCC1.2166. During use of the composite preparation, the injection stage is divided into four slugs which are completed discontinuously, and finally even oil layer displacement and crude oil elution are achieved. The microbial composite preparation has the advantages that oil field water injection efficiency can be effectively improved so as to increase crude oil recovery efficiency, the preparation is non-hazardous to environments and human bodies, promising the application prospect in the field of low-permeability reservoir microbial enhanced oil recovery, simple in process, low in production cost and good in environmental adaptability to low-permeability reservoirs, and crude oil recovery efficiency can be increased evidently.

This present invention relates to compositions and methods of microbial enhanced oil recovery using Bacillus subtilis strains. The invention also relates to compositions and methods for performing oil degradation with Bacillus subtilis strains. The compositions and methods of the present invention are also used for enhanced commercial biosurfactant and enzyme production.

This invention discloses a Bacillus subtilis strain L-510 CGMCC No.1563 and the application thereof. The lab analysis shows that the changes of the crude oil processed by this strain have the following characteristics: (1)the rang of the light components is broadened, the content of the non-hydrocarbon components is averagely increased by 2.09%, and the content of asphaltene is averagely decreased by 1.29%, (2) the rate of wax is averagely decreased by 17.56%, and the rate of colloid is averagely decreased by 26.54%, (3)the flow capacity of the crude oil is improved, and the initial apparent viscosity number of the viscosity-temperature curve is decreased by 50% campared to the value before the procession. The result of the microbial huff and puff field test using this strain conducted in Pubei Oilfield shows that, in the four months after being processed by this strain, the effective wells are upto 80%, the liquid output is increased by 44.8%, and the daily oil production is increased by 47.4%. This strain can be widely used in reservoir engineering field, especially in the Microbial Enhanced Oil Recovery(MEOR) field.

The invention relates to a microorganism circulating oil recovery method adopting a mode of continuously filling low-concentration nutrient solution, and is applied to microbial enhanced oil recovery for oil fields. Firstly, the number of microorganisms in the stratum reaches 105 to 107 pieces / mL by filling high-concentration bacteria solution and / or nutrient solution into a target oil reservoir so as to establish an oil reservoir microorganism field; and then the growth and propagation of the microorganisms in the stratum are kept by continuously filling the low-concentration nutrient solution with the concentration of 0.2 percent so as to fulfill the aim of sustained action of the microorganisms and metabolic products thereof on crude oil in the oil reservoir, prolong the period of validity of microorganism oil recovery and improve the input-output ratio. Field application shows that the adoption of the mode of continuously filling low-concentration nutrient solution with the concentration of 0.2 percent can effectively promote the growth and propagation of the microorganisms and avoid large fluctuation of the number of the microorganisms, which is caused by a slug filling mode; and according to the method, under the condition that the cost is not increased, the recovery ratio is improved by 7.0 percent.

The invention relates to the technical field of microbial enhanced oil recovery, and in particular relates to an extraction method of oil reservoir microbial genome DNA. The method comprises the following steps: removing interference of crude oil in samples with extraction by means of organic solvent extraction; releasing microbes on an oil-water interface; filtering water phase by using a hollow fiber membrane to enrich microbes; removing organic macromolecular components such as proteins by phenol and chloroform in cooperation with lysis cells such as lysozyme, helicase, protease and lauryl sodium sulfate according to a physical wall-breaking method under a high-salinity condition; subsequently, performing nucleic acid extraction and precipitation to obtain the oil reservoir microbial genome DNA. The method is quick and simple, and the whole extraction process can be completed in 10 hours; the extraction efficiency is high, and the purity of the obtained genome is high.

The invention belongs to the technical field of MEOR (Microbial Enhanced Oil Recovery), and particularly relates to a method for optimizing microbial oil displacement site injection process parameters. The method comprises the following steps of (1) screening site injection process parameters; (2) determining a value range of the site injection process parameters; (3) optimizing the site injection process parameters. The method disclosed by the invention has the advantages of simpleness in step, strong logicality, strong operability, high reliability and accuracy of an optimized result and the like, and thus the method can be widely applied to laboratory tests and site tests of MEOR.

Disclosed herein is a method to control distribution of injected microorganisms, nutrients or other chemical treatment fluids from a single injection well to multiple production wells. The sweep efficiency is improved preventing premature production of fluids from production wells with short breakthrough times. This is a useful technique for treatment of subterranean target sites for practice of Microbial Enhanced Oil Recovery and bioremediation.

This invention relates to compositions and methods of microbial enhanced oil recovery using biochemical-producing microbes. In specific embodiments, the methods of the subject invention comprise applying a bio surfactant-producing bacteria and / or a growth by-product thereof to an oil-producing site. In preferred embodiments, the bacteria is a strain of Bacillus in spore form. In some embodiments, the methods further comprise applying the bacteria with a yeast fermentation product, an alkaline compound, a polymer, a non- biological surfactant, and / or one or more chelating agents. Advantageously, the subject invention can be useful for stimulating the flow of oil from a well, as well as dissolving scale present in an oil-bearing formation.

The invention belongs to the technical field of tertiary oil recovery, and particularly relates to a method for increasing the crude oil recovery rate through indigenous microbial drive. The method specifically comprises the following steps that oil reservoirs are screened; high-permeability oil layers, middle-permeability oil layers and low-permeability oil layers are determined; activating agent systems are determined; field injection processes are determined; and field tests are conducted. Different activating agent systems and field injection processes are selected according to the oil layers with different permeability of the target oil reservoirs, indigenous microorganisms of different oil layers are well activated, and therefore the field test effect of the indigenous microbial drive oil reservoirs can be effectively improved. Meanwhile, the method has the characteristics of being simple in process and high in pertinence and operability. Accordingly, the method can be widely applied to the technical field of microbial enhanced oil recovery.