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Novel thermophilic and salt-resistant strain capable of degrading raw oil and generating emulsifying agent and application thereof

A biological emulsifier, crude oil technology, applied in the fields of energy biotechnology and environmental biology, can solve the problem of not many bacterial species, and achieve the effects of strong emulsification activity, increase of saturated hydrocarbon content, and improved properties

Inactive Publication Date: 2015-05-06
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among some petroleum microorganisms that have been isolated so far, there are not many thermophilic and salt-tolerant strains that can degrade crude oil under aerobic or anaerobic conditions and can produce biological emulsifiers.

Method used

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  • Novel thermophilic and salt-resistant strain capable of degrading raw oil and generating emulsifying agent and application thereof
  • Novel thermophilic and salt-resistant strain capable of degrading raw oil and generating emulsifying agent and application thereof
  • Novel thermophilic and salt-resistant strain capable of degrading raw oil and generating emulsifying agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 The separation of Anoxybacillus rupiensis WJ-4 without oxygen

[0045] The screening and isolation of Anoxybacillus rupiensis WJ-4 mainly include sampling and enrichment culture, screening and purification, and identification. The specific methods are as follows:

[0046] (1) Sampling and enrichment culture

[0047] Take an appropriate amount of formation water from Longhupao oil reservoir in Daqing and insert it into the enrichment medium. The enrichment medium uses inorganic salt medium (g / L): (NH 4 ) 2 SO 4 4.5, Starch 2, Na 2 HPO 4 2.0, MgSO 4 ·7H 2 O 0.5, NaCl 1.5, FeCl 2 0.5, yeast powder 0.3, liquid paraffin 5. pH 7.2-7.5, sterilized at 121°C, cultured on a shaker at 60°C at 200rpm for 15-20 days.

[0048] (2) Screening and purification of Anoxybacillus rupiensis WJ-4

[0049] The enriched culture solution in step (1) was diluted step by step, and the dilution factor was taken as 10 4 、10 5 The bacterial solution was evenly spread on the LB ...

Embodiment 2

[0058] Example 2 Anoxybacillus rupiensis WJ-4 uses waste crude oil and molasses as carbon source to produce surfactant

[0059] The Bacillus aerobicus WJ-4 of the present invention has the property of high-yield biological emulsifier, no matter utilizes oil-soluble carbon source or water-soluble carbon source, all can synthesize biological emulsifier; Especially in industrial waste or waste (Petroleum pollution or molasses) as a carbon source for the fermentation of bioemulsifiers produced unexpected technical effects.

[0060] see Figure 4 , after the crude oil culture medium was inserted into WJ-4 cells and cultured for 10 days, the oil layer was dispersed in the water phase in fine powder form, no crude oil adhered to the bottle wall, the water phase was brownish black, the oil-water interface was unclear, and no stratification after standing still ( flask on the right in the figure). However, the water phase of the crude oil medium without bacterial cells was still clea...

Embodiment 3

[0065] Example 3 Application of Anoxybacillus rupiensis WJ-4 and its fermented liquid in degradation of crude oil

[0066] In order to study the properties of strain WJ-4 to degrade crude oil, this example uses this strain to carry out microbial degradation experiments on Daqing Longhupao crude oil, and analyzes the impact of bacterial degradation on crude oil saturated hydrocarbons, aromatics, colloids, and asphaltenes in crude oil. The influence of the relative content and internal composition, and the emulsification performance of the emulsifier formed during the degradation process on crude oil.

[0067] Surfactant Analysis

[0068] The fermentation kinetic curves of Anoxybacillus rupiensis WJ-4 strain under aerobic and anaerobic conditions using waste crude oil and molasses as carbon sources are as follows: Figure 5A and Figure 5B shown. It can be seen from the figure that Anoxybacillus rupiensis WJ-4 strain can efficiently utilize crude oil and molasses to synthesiz...

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Abstract

The invention provides a novel thermophilic and salt-resistant strain capable of degrading raw oil and generating an emulsifying agent and an application thereof. Particularly, the invention relates to anoxybacillus rupiensis with the collection number CGMCC No.6614 and an anaerobic bacillus preparation containing the anoxybacillus rupiensis. The invention also relates to an application of the anoxybacillus rupiensis or the preparation thereof in fermenting, culturing and producing biological surface active substances, an application in degrading the raw oil and an application in displacing oil. The anoxybacillus rupiensis strain can grow under the high-temperature and high-salt environment and can adopt the raw oil and molasses as a carbon source to generate the macromolecular emulsifying agent under the aerobiotic and anaerobic conditions; and the biological emulsifying agent has strong emulsifying capability, and also has the effects of effectively improving the solubility of alkane or the raw oil in water and obviously promoting the degradation of the strain to the raw oil. The strain and the emulsifying agent generated thereby can be used for microbial enhanced oil recovery and can increase the oil recovery rate.

Description

technical field [0001] The invention belongs to the fields of energy biotechnology and environmental biotechnology. Specifically, the invention relates to an anoxybacillus rupiensis and its application. The strain can use crude oil and molasses as carbon sources and energy sources for growth, while The biological emulsifier is produced, and the bacterial strain and the biological emulsifier produced by it can be used in microbial oil recovery to increase oil recovery rate. Background technique [0002] Petroleum is a non-renewable resource. With the increasing shortage of petroleum resources and the continuous increase of exploration costs, after primary oil recovery and secondary oil recovery, there are still about 60%-70% of the ground that cannot be extracted. The shortage of oil has gradually been unable to meet people's increasing demand for oil resources. In recent years, the tertiary oil recovery method represented by Microbial Enhanced Oil Recovery (MEOR) technology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C09K8/582C12R1/01
CPCC09K8/582C12P1/04C12N1/205C12R2001/01
Inventor 夏文杰崔庆锋董汉平俞理董浩谢坤王萍
Owner PETROCHINA CO LTD
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