418 results about "Nitroso" patented technology

The invention provides a betahistine oral solid preparation with improved stability and a preparation method thereof. The oral solid preparation comprises betahistine or a salt thereof and a non-inorganic salt antioxidant. In addition, the invention also discloses a preparation method of the medicinal composition. According to the technical scheme provided by the invention, the problems of control and growth of N-nitroso betahistine in a betahistine solid preparation are effectively solved.

The invention discloses an ammonia nitrogen detection system and an application method thereof, and ammonia nitrogen detection test paper, the ammonia nitrogen detection system is prepared by the following steps: step SA1) preparing a dipping mixed solution, the dipping mixed solution comprises: a potassium sodium tartrate solution with a concentration of 3-60 g / L; a sodium salicylate solution with a concentration of 20-320 g / L; the concentration of the sodium nitroprusside solution is 0.1 to 0.8 g / L; and deionized water. On the basis of the principle of salicylic acid-pypocholoride spectrophotometry, the impregnated test paper, the reagent tube and the standard colorimetric card are combined, and the rapid ammonia nitrogen detection system special for medium-concentration sewage detection is successfully developed. The toxicity problem of mercury iodide in a Nessler reagent method and the toxicity problem of phenol in a phenol-pypocholoride spectrophotometric method are avoided, main reagents are non-toxic and harmless to a human body, interference of metal ions in a water sample can be effectively eliminated by adopting sodium potassium tartrate tetrahydrate as a chelating agent, and the detection stability and accuracy are improved.

The invention belongs to the field of electrochemical sensing, and particularly relates to construction and application of an electrochemical luminescence sensor for detecting the content of N-nitrosodimethylamine with high sensitivity. The Cas12a enzyme improves the specificity and realizes signal amplification through the recognition mechanism and trans-cleavage capability of the Cas12a enzyme. The ATT-AuNCs is used as an efficient electrochemical luminescence emitter, so that the transition of a signal is promoted. After a sample is added, the Cas12a-crRNA compound can specifically recognize a DNA activator to trigger cutting of non-specific DNA which is modified on a glassy carbon electrode and is marked by ferrocene, so that the electrochemical luminescence intensity is increased. The NDMA in the sample can be detected through the method, so that the content of nitrosamine in food can be detected. The CRISPR / Cas12a is combined with an electrochemical luminescence technology, so that a sensitive and rapid NDMA detection means is provided, and a new idea is provided for detecting the content of cancerogenic substances in various foods.

The present invention provides a pharmaceutical composition, the composition comprises a therapeutically effective amount of bumetanib, one or more specific antioxidants and other necessary one or more pharmaceutically acceptable pharmaceutic adjuvants, and the specific antioxidants are selected from one or more of fat-soluble vitamin C derivatives or phenolic antioxidants. In addition, the invention also discloses a method for improving the stability of the bumetanib pharmaceutical composition. According to the technical scheme, the problems of generation and growth of N-nitroso bumetanib and other related substances in the production and storage process of the bumetanib solid preparation are effectively solved, and the drug stability and the medication safety are remarkably improved.

The invention discloses a detection method for determining N-nitroso-Vonoprasan in Vonoprasan fumarate, and belongs to the technical field of medicine detection.The detection method comprises the steps that a solution is prepared, and detection is conducted; the detection comprises the following steps: determining a test solution and a reference solution by adopting a liquid chromatography-mass spectrometry method, recording a chromatogram, and calculating the content of N-nitroso-Vonorazan according to the chromatogram and an external standard method; according to the method, a triple quadrupole rod liquid phase mass spectrometer is adopted; a chromatographic column adopts octadecyl silane bonded silica gel as a filling agent, the specification of the chromatographic column is Poroshell 120EC-C18, 1.9 mu m, 50 mm * 3.0 mm; a mobile phase is composed of a mobile phase A and a mobile phase B, and gradient elution is adopted; the ultra-high pressure chromatographic column is adopted, the operation time is shorter, the signal-to-noise ratio is higher, the detection sensitivity is higher, the detection cost is lower, and the detection requirement can be met on a low-end triple quadrupole mass spectrum.

The present invention relates to: a temperature-sensitive hydrogel for cancer treatment; a photothermal composition comprising the hydrogel as an active ingredient; and a preparation method for the temperature-sensitive hydrogel for cancer treatment. The temperature-sensitive hydrogel of the present invention includes gold nanostars as active ingredients, and thus can generate heat by light irradiation, thereby exhibiting a photothermal therapy effect. In addition, the hydrogel temperature increases so that the release of nitric oxide (NO) from S-nitrosocysteine can be induced. In addition, the temperature-sensitive hydrogel of the present invention includes S-nitrosocysteine as an active ingredient so that, upon a temperature rise due to a photothermal reaction, the penetration of drugs into a tumor site can be improved by the release of NO, and at the same time, apoptosis of cancer cells can be directly caused; and includes an immunotherapy agent as an active ingredient so that tumor size can be effectively suppressed even with the passage of time. Therefore, as a material that enables complex treatment combined with photothermal therapy and immunotherapy, the temperature-sensitive hydrogel of the present invention having the aforementioned effect can be usefully utilized in the field of medicine for cancer treatment.

The disclosure relates to a method of producing a multi-metal catalyst film and of producing a reactor system for catalytic removal of a wide variety of contaminants (for example, nitrate, nitrite, perchlorate, chlorate, chromate, selenate, chlorophenols, 2,4-D, dicamba, atrazine, trichloroacetic acid, bromochloroiodomethane, NDMA, TCE, TCA, chloroform, freons, RDX, HMX, TNT, PFOA, and PFOS) from water and wastewater. The disclosure also relates to a method of using the multi-metal catalyst for the removal of such contaminants and a system comprising the multi-metal catalyst film for removing such contaminants.

A pharmaceutical composition comprising sitagliptin or a pharmaceutically acceptable salt thereof, wherein the composition comprises less than 5 ppm of 7-Nitroso-3-(trifluoromethyl)- 5,6,7,8-tetrahydr

The invention provides a betahistine hydrochloride pharmaceutical preparation and a preparation method thereof. The betahistine hydrochloride pharmaceutical preparation comprises conjugated linoleic acid, betahistine hydrochloride and auxiliary materials. Conjugated linoleic acid and betahistine hydrochloride are combined together for the first time, and conjugated double bonds of conjugated linoleic acid can be specifically combined with nitrite to block the generation of nitrosamine, so that the content of N-nitrosobetahistine can be remarkably reduced. Based on the chemical characteristics of conjugated linoleic acid, conjugated linoleic acid is used as a functional additive, targeted removal of nitrosamine impurities is achieved, and meanwhile the situation that exogenous toxic substances are introduced to affect the drug effect of betahistine hydrochloride is avoided. In the experiment process, it is also accidentally found that after the conjugated linoleic acid is added, the positive effect on cerebrovascular diseases is also achieved.

The invention discloses lactobacillus brevis and application thereof, and belongs to the technical field of food microorganisms. The invention provides a lactobacillus brevis CHOL1, which is separated from pickled Chinese cabbages, can be applied to food through safety evaluation, has relatively strong nitrite degradation capability, can react with myoglobin to generate nitrosylmyoglobin, and can be used for degrading nitrite in food. After being inoculated into the fermented meat product, the strain can reduce nitrite and nitrosamine residues in the fermented meat product and improve the color and luster of the meat product at the same time. As a leavening agent, the strain can improve the quality and safety of fermented meat products, and can be used as a natural effective substitute for partially replacing nitrite in the fermented meat products.

This invention relates to N-(3-(6-Amino-5-(2-(N-methylacrylamido)ethoxy)pyrimidin-4-yl)-5-fluoro-2-methylphenyl)-4-cyclopropyl-2-fluorobenzamide drug substance substantially free of a nitrosamine impurity, e.g. the nitrosamine impurity N-(3-(6-amino-5-(2(methyl)(nitroso)amino)ethoxy)pyrimidin-4-yl)-5-fluoro-2-methylphenyl)-4-cyclopropyl-2-fluorobenzamide and novel processes of preparation thereof. The invention further relates to pharmaceutical composition comprising N-(3-(6-Amino-5-(2-(N- methylacrylamido)ethoxy)pyrimidin-4-yl)-5-fluoro-2-methylphenyl)-4-cyclopropyl-2-fluorobenzamide, wherein the composition is substantially free of a nitrosamine impurity, e.g. the nitrosamine impurity N-(3-(6-amino-5-(2(methyl)(nitroso)amino)ethoxy)pyrimidin-4-yl)-5-fluoro-2-methylphenyl)-4-cyclopropyl-2-fluorobenzamide. Pharmaceutical products containing said drug substance and compositions are also disclosed, as well as methods for preparing said drug substance, pharmaceutical compositions and products.

The invention relates to the technical field of medicines, relates to a preparation method and application of a genotoxic impurity standard substance in acarbose, and in particular relates to a preparation method and application of a genotoxic impurity N-nitrosyl acarbose in an acarbose tablet. According to the method, the nitrosamine new structure compound NNA is prepared for the first time, the genotoxic impurity NNA with high purity can be effectively prepared through the method, the blank of an impurity preparation method at present is filled, the preparation process is simple, and a large number of high-purity NAA impurity standard substances can be prepared. The invention provides a UHPLC-Orbitrap-HRMS (Ultra High Performance Liquid Chromatography-Orbitrap-High Resolution Mass Spectrometry) combined technology, so that the quality control of the NNA in the acarbose tablet is effectively realized, the side reaction of medication is reduced, and the medication safety is ensured to a certain extent. Meanwhile, reference is provided for quality evaluation of other starting materials or intermediates capable of generating NNA.

The invention provides a construction method and application of a mouse gastric cancer spontaneous tumor model, and the construction method comprises the following steps: reducing or inhibiting the expression quantity of a TP53 gene and / or the activity of a P53 protein in a mouse body, and then giving a drug to the mouse for chemical induction to obtain the mouse gastric cancer spontaneous tumor model. Wherein the drug contains sodium nitrite and ethyl sarcosinate, and / or the drug contains an N-nitroso compound. According to the construction method of the mouse gastric cancer spontaneous tumor model, the construction of the C57 mouse gastric cancer spontaneous tumor model by using the NESS is proposed for the first time, and the tumor formation rate can reach 75% or above; the tumor formation period is short and is about 3-4 months, and the construction cost is low.

The invention relates to the technical field of hydrogel materials, in particular to NO controlled release polyvinyl alcohol / sodium alginate hydrogel and a preparation method thereof. The preparation method comprises the following steps: mixing and stirring a polyvinyl alcohol solution and a sodium alginate solution to obtain a mixed solution; s-nitroso-N-acetyl-penicillamine and graphene oxide are added into the mixed solution, the obtained solution is poured into a mold, hydrogel is obtained after freezing, the hydrogel is taken out of the mold and unfrozen at the room temperature, freezing and thawing cycles are repeated, and the polyvinyl alcohol / sodium alginate hydrogel capable of controllably releasing NO is obtained. According to the NO controlled release polyvinyl alcohol / sodium alginate hydrogel and the preparation method thereof, the prepared NO controlled release polyvinyl alcohol / sodium alginate hydrogel has excellent antibacterial property, conductivity, slow release property, mechanical property and biocompatibility, and the technical problems that existing hydrogel is insufficient in mechanical property and poor in antibacterial property are solved.

The invention belongs to the technical field of medicine detection, and particularly relates to a method for detecting a nitroso compound in a raw material medicine in a riptacaine emulsifiable paste, which comprises the following steps: preparing a test sample: taking the riptacaine emulsifiable paste, adding a 5mol / L sodium hydroxide solution, uniformly mixing, then adding a 5mol / L hydrochloric acid solution, and uniformly mixing; diluting with a solvent with the pH value of 7.20 + / -0.02, and then centrifugally filtering to obtain a test sample; wherein the solvent is a mixed solvent composed of monopotassium phosphate and acetonitrile; liquid chromatography separation: the tested sample is separated by liquid chromatography, and a mobile phase A is a 0.2% formic acid solution; the mobile phase B is methanol; the chromatographic column is SB-C18, 2.1 * 100 mm, 1.7 [mu] m; and mass spectrum detection: detecting the substance separated by the liquid chromatography by using a mass spectrometer to obtain the content of the nitroso compound. The detection method is efficient, sensitive and excellent in reproducibility.

The invention discloses a fermentation process for shortening a fermentation period and efficiently synthesizing Ectoine. The fermentation process comprises the following steps: screening and activating high-yield strains, preparing a fermentation culture medium, performing fermentation culture, performing sectional dissolved oxygen regulation and control, performing double-stage pH dynamic regulation and control, performing carbon-nitrogen ratio dynamic regulation based on metabolic flow analysis, performing osmotic pressure gradient induction in the later stage of fermentation, and extracting and purifying a product. According to the fermentation process for shortening the fermentation period and efficiently synthesizing the Ectoin, the expression quantity of key enzymes for synthesizing the Ectoin is increased by 80% or above compared with that of an original strain through the Halomonassp.ECT-09 strain bred through ultraviolet-nitrosoguanidine compound mutation, the basic yield reaches 35 g / L, a foundation is laid for high yield, and the method is suitable for industrial production. A glucose-trehalose mixed carbon source and a yeast extract-ammonium nitrate mixed nitrogen source are adopted, and an L-aspartic acid-L-asparagine mixed precursor is added, so that the metabolic pathway is shortened, and the loss of an intermediate product is reduced.

Disclosed is a process for the synthesis of mono- and bis-nitrosylated propanediols, as well as compositions and pharmaceutical formulations that includes the compounds. The process proceeds by reacting a corresponding propanediol that is not nitrosylated with a source of nitrite, optionally in the presence of a suitable acid. When the source of nitrite is an organic nitrite, reacting step is performed in a suitable organic solvent, and when the source of nitrite is an inorganic nitrite, the reacting step is performed in a bi-phasic solvent mixture comprising an aqueous phase and a non-aqueous phase. Also disclosed are methods of treating a condition wherein administration of nitric oxide (NO) has a beneficial effect by administering said compounds, compositions or formulations.

The invention belongs to the technical field of noble metal catalyst preparation, and particularly relates to a preparation method of high-purity tetraammineplatinum nitrate. According to the method, potassium tetranitroplatinate is prepared from chloroplatinic acid and potassium nitrite, then potassium hexanitroplatinate is prepared, and finally platinum nitrate tetraammine is prepared, so that the method uses fewer types of raw materials, does not use expensive reagents, has fewer steps, is simple and convenient to operate, is easy to realize large-scale production, and is suitable for industrial production. The yield of the prepared platinum tetraamminenitrate is greater than or equal to 94.1%, the purity of the platinum tetraamminenitrate is greater than or equal to 99.3%, and the platinum content is greater than or equal to 41.9%.

The invention provides a preparation method of a nitrosamine impurity, and belongs to the technical field of heterocyclic compounds. The clarithromycin related nitrosamine impurity is 3 '-N-demethyl-3'-N-nitroso-6-O-methyl erythromycin, and is prepared by carrying out demethylation and nitrosation on clarithromycin. The clarithromycin intermediate erythromycin oxime related nitrosamine impurity is 3 '-N-demethyl-3'-N-nitroso erythromycin A (E)-9-oxime, and the clarithromycin intermediate erythromycin oxime related nitrosamine impurity is prepared from erythromycin A (E)-9-oxime through demethylation, etherification, nitrosation and hydrolysis.

The invention discloses an ultrahigh-performance offshore wind power grouting material as well as a preparation method and application thereof. The ultrahigh-performance offshore wind power grouting material is prepared from the following components in parts by weight: 600 to 800 parts of cement, 100 to 200 parts of a viscosity reducer, 75 to 150 parts of silica fume, 75 to 150 parts of ground zeolite powder, 6 to 8 parts of a water reducing agent, 1 to 2 parts of a de-foaming agent, 0.1 to 0.5 part of a plastic expanding agent, 50 to 80 parts of an anti-cracking agent, 700 to 900 parts of carborundum and 155 to 170 parts of water, the cement is 52.5-grade white portland cement; the viscosity reducer is prepared from the following components in percentage by weight: 30 percent to 50 percent of microbeads, 15 percent to 35 percent of limestone powder, 20 percent to 30 percent of superfine mineral powder and 5 percent to 15 percent of metakaolin, and the viscosity reducer is prepared from the following components in percentage by weight: 30 percent to 50 percent of microbeads, 15 percent to 35 percent of limestone powder, 20 percent to 30 percent of superfine mineral powder and 5 percent to 15 percent of metakaolin. The plastic expanding agent is composed of an azo compound plastic expanding agent and a nitroso compound plastic expanding agent in a weight ratio of (0.5-2): 1. The ultrahigh-performance offshore wind power grouting material is high in early strength, good in fluidity and excellent in durability, has the micro-expansion characteristic, and is simple in preparation process and low in cost.

The invention discloses a method for determining nitrosamine impurity N-nitrosobumetanib in bumetanib, which comprises the following steps: using a high performance liquid chromatograph and an ultraviolet detector, using octadecyl silane bonded silica gel as a chromatographic column filler, using formic acid solution as a mobile phase A, using methanol as a mobile phase B, the content of the nitrosamine impurity N-nitrosobumetanib in the bumetanib is determined through gradient elution. The novel high performance liquid chromatography is designed, the content of the nitrosamine impurity N-nitrosobumetanib in the bumetanib can be accurately measured, the blank that a method for measuring the N-nitrosobumetanib in the bumetanib bulk drug does not exist at present is filled, and the analysis method is verified; the method for determining the nitrosamine impurity N-nitrosobumetanib in the bumetanib, provided by the invention, is good in repeatability, high in selectivity, good in accuracy and high in precision and sensitivity; according to the invention, the quality control of the medicine can be effectively enhanced.

The invention belongs to the technical field of medicines, and particularly relates to bletilla striata polysaccharide hydrogel as well as a preparation method and application thereof. The BSP-MA / HA-NB hydrogel is prepared by screening the variety, dosage and preparation process of each component in the hydrogel, and the hydrogel is prepared from the following raw materials: methacrylated bletilla striata polysaccharide, o-nitrobenzaldehyde modified hyaluronic acid and the like. The adhesive property and the mechanical property of the hydrogel are obviously improved; the long-acting slow-release effect of the medicine components is achieved, the proliferation activity and migration ability of the bone marrow mesenchymal stem cells are promoted, and the bone marrow mesenchymal stem cells have good application prospects in the aspect of bone defect repair treatment.

The invention provides a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) detection method for N-nitroso landiolol in a landiolol hydrochloride bulk drug. The method comprises the step of carrying out qualitative detection or quantitative analysis on a potential nitrosamine impurity-N-nitroso landiolol in a landiolol hydrochloride bulk drug by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), wherein the structural formula of the N-nitroso landiolol is shown as a formula I in the specification. The detection method disclosed by the invention is high in sensitivity, strong in specificity and good in repeatability, can be used for rapidly and effectively detecting N-nitroso landiolol in the landiolol hydrochloride bulk drug, and has important significance on quality control of the landiolol hydrochloride bulk drug. ; formula I.

The invention discloses a method for detecting an N-nitroso compound in vortioxetine hydrobromide, and the N-nitroso compound is 2, 2 '-(nitroso amino) bis-acetonitrile. According to the method, a high performance liquid chromatography is adopted, an octadecyl silane bonded silica gel filler chromatographic column and an ultraviolet detector are adopted, a mobile phase A is 0.05% phosphoric acid aqueous solution, a mobile phase B is methanol, and gradient elution is adopted. The method has good specificity and sensitivity.

The invention provides a reagent for flotation separation of fluorite and gangue minerals, and belongs to the technical field of mineral separation. The reagent for flotation separation of fluorite and gangue minerals comprises a collecting agent, a regulating agent and an inhibitor, the collecting agent comprises the following components in parts by mass: 10 to 40 parts of alkyl phosphoric acid, 60 to 80 parts of alkanoylamino carboxylic acid (sulfonic acid) and 1 to 10 parts of alpha-nitroso-beta-naphthol. The regulator is aluminum potassium sulfate; the inhibitor is prepared from the following components in parts by mass: 40 to 50 parts of water glass, 40 to 45 parts of lignosulfonic acid and 5 to 20 parts of bitter ketonic acid. According to the method, efficient recovery of fluorite is achieved through the synergistic effect of the collecting agent, the adjusting agent and the inhibitor, the grade of CaF2 in fluorite concentrate exceeds 96%, and the recovery rate reaches 85% or above.

The invention discloses a method for determining nitrosamine impurity N-nitrosociprofloxacin in ciprofloxacin lactate, and relates to the field of substance detection methods, the method adopts a liquid chromatography-mass spectrometry technology, and the liquid chromatography condition comprises that a mobile phase is a mixture of a formic acid solution with the concentration of 0.08%-0.12% and acetonitrile, and the volume ratio of the formic acid solution to the acetonitrile is (45-55): (45-55). According to the present invention, the liquid chromatography (LC) technology and the mass spectrometry (MS) technology are combined, such that the efficient separation ability of the liquid chromatography and the high sensitivity detection ability of the mass spectrometry are combined, the detection sensitivity and the detection accuracy are significantly improved, and the strict requirements of drug quality control and safety are met.

This application relates to a rifapentine-containing pharmaceutical composition and its preparation method. The rifapentine-containing granules are prepared by dry granulation, which reduces the content of impurities present in existing pharmaceutical compositions, especially the content of the genotoxic impurity 1-cyclopentan-4-nitrosopiperazine, thereby improving the safety and efficacy of the drug.

The invention provides a sterile, injectable pharmaceutical composition which includes a therapeutically effective amount of ketamine or a pharmaceutically acceptable salt thereof, a nitrite scavenger, a tonicity agent, and water, wherein the composition contains less than about 300 ppb of an N-nitrosoketamine impurity. The invention also provides a pharmaceutical product which includes a container and the composition of the invention aseptically sealed therein. The invention further provides a process for preparing the composition and product of the invention.

The present invention relates to a method for preparing nitrosamine impurities in vonoprazan fumarate, belonging to the technical field of chemical synthesis. By mixing vonoprazan with an organic solvent and reacting the mixture with a nitrosating agent at room temperature, the nitrosamine impurities prepared by this method have a purity exceeding 85%. This method solves the problem of preparing nitrosamine impurities and also provides a reference substance for the study of impurities in vonoprazan fumarate.

The invention discloses a detection method of vitamin K1, and belongs to the technical field of biological detection. The problems that a vitamin K1 detection method is tedious in operation, prone to being influenced by a matrix, low in sensitivity and the like are solved. The detection method comprises the following steps: (1) preparing a standard substance intermediate working solution, an isotope internal standard substance solution and a standard curve working solution of the vitamin K1; (2) treating a serum sample and a standard curve working solution: adding a 2-nitroso pyridine solution to carry out derivatization reaction; and (3) detecting the vitamin K1 by using a high performance liquid chromatography-tandem mass spectrometry method. According to the method, the 2-nitroso pyridine is used as a derivatization reagent, the vitamin K1 and the 2-nitroso pyridine are subjected to a Diels-Alder reaction to generate a new six-membered heterocycle containing N and O, the 2-nitroso pyridine can efficiently and completely react with the vitamin K1 in only 10 minutes at room temperature, the detection steps are simplified, the sample pretreatment time is shortened, and the detection efficiency is improved.